a spreadable, non-integrative and high copy number shuttle vector for sulfolobus solfataricus based on the genetic element pssvx from sulfolobus islandicus.the pssvx genetic element from sulfolobus islandicus rey15/4 is a hybrid between a plasmid and a fusellovirus, able to be maintained in non-integrative form and to spread when the helper ssv2 virus is present in the cells. in this work, the satellite virus was engineered to obtain an escherichia coli-sulfolobus solfataricus shuttle vector for gene transfer and expression in s.solfataricus by fusing site-specifically the pssvx chromosome with an e.coli plasmid replicon and the ampicillin resistan ...200616971457
relationships between fuselloviruses infecting the extremely thermophilic archaeon sulfolobus: ssv1 and ssv2.the fusellovirus ssv2 from an icelandic sulfolobus strain was isolated, characterized and its complete genomic sequence determined. ssv2 is very similar in morphology, replication, genome size and number of open reading frames (orfs) to the type virus of the family, ssv1 from japan, except in its high level of uninduced virus production. the nucleotide sequences are, however, only 55% identical to each other, much less than related bacteriophage, related animal viruses and the rudiviruses of sul ...200312798235
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