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detection of hepatopancreatic parvovirus (hpv) infection in penaeus monodon using pcr-elisa.a rapid and sensitive pcr-elisa has been developed for detection of hepatopancreatic parvovirus (hpv) in penaeus monodon. the specific primer set amplified 156 bp fragment and could detect as a little as 0.01 fg of purified hpv dna which equivalent to three viral particles. no cross-reactivity was observed when nucleic acid templates from white spot syndrome virus, yellow-head virus, monodon baculovirus and shrimp were tested. the crude dna simple prepared from hepatopancreas can be used as dna ...200212490141
characterization of a shrimp serine protease homolog, a binding protein of yellow head virus.a serine protease homolog (sph) cdna namely sph516 was identified via a yeast two-hybrid screen between yellow head virus (yhv) proteins and hemocyte proteins of the black tiger shrimp penaeus monodon. initially, the c-terminal region of sph516 (sph516-c) was found to interact with a putative metal ion-binding domain (mib) encoded by open-reading of frame orf1b of the yhv genome. subsequently, the full-length of sph516 cdna was obtained using 5' rapid amplification of cdna ends (5' race) and it ...200717451807
multiplex rt-pcr assay for simultaneous detection of six viruses of penaeid shrimp.in the present study, multiplex reverse transcription-polymerase chain reaction (mrt-pcr) was developed for simultaneously detection of six major shrimp viruses including yellow-head virus (yhv), white spot syndrome virus (wssv), taura syndrome virus (tsv), hepatopancreatic parvovirus (hpv), infectious hypodermal and hematopoietic necrosis virus (ihhnv) and monodon baculovirus (mbv). the six primer sets could amplify viral nucleic acids resulting in pcr products with different sizes. they were h ...200818406570
successful propagation of shrimp yellow head virus in immortal mosquito cells.research on crustacean viruses is hampered by the lack of continuous cell lines susceptible to them. to overcome this problem, we previously challenged immortal mosquito and lepidopteran cell lines with shrimp yellow head virus (yhv), followed by serial, split-passage of whole cells, and showed that this produced cells that persistently expressed yhv antigens. to determine whether such insect cultures positive for yhv antigens could be used to infect shrimp penaeus monodon with yhv, culture supe ...201020597433
glycosylation of gp116 and gp64 envelope proteins of yellow head virus of penaeus monodon shrimp.yellow head virus (yhv) is a highly virulent pathogen of penaeus monodon shrimp that is classified in the genus okavirus, family roniviridae, in the order nidovirales. separation of virion proteins treated with peptide-n-glycosidase-f (pngase-f) in sds-polyacrylamide gels and the use of glycoprotein-specific staining methods indicated that the gp116 and gp64 envelope glycoproteins possess n-linked rather than o-linked glycans. competitive binding inhibition of lectins with various oligosaccharid ...201020554800
impact of yellow head virus outbreaks in the whiteleg shrimp, penaeus vannamei (boone), in thailand.yellow head virus (yhv) is known as a major pathogen in the black tiger shrimp, penaeus (penaeus) monodon. it can also cause serious mortality in farmed whiteleg shrimp, penaeus (litopenaeus) vannamei. however, there is no published information on the economic and/or production impact of the disease in p. vannamei. shrimp with gross signs of yhv disease (faded body colour and 60-70% mortality) were observed in 20 study farms rearing p. vannamei in the central part of thailand from the end of 200 ...201020158577
changes in the histological organization and spheroid formation in lymphoid organ of penaeus monodon infected with yellow head virus.the changes in the histological and three dimensional organizations of lymphoid organ and characteristics of lymphoid cells after chronic infection with yellow head virus (yhv) in penaeus monodon were investigated. the vascular cast of infected lymphoid organ showed less branching and dramatically shortened terminal capillaries that formed the lymphoid tubules, and only large stumps of these lymphoid tubules remained. this might occur because the terminal ends of the tubules were damaged from yh ...200818796333
genetic diversity in the yellow head nidovirus complex.penaeus monodon shrimp collected from across the indo-pacific region during 1997-2004 were screened for the presence of yellow head-related viruses. phylogenetic analyses of amplified orf1b gene segments identified at least six distinct genetic lineages (genotypes). genotype 1 (yhv) was detected only in shrimp with yellow head disease. genotype 2 (gav) was detected in diseased shrimp with the less severe condition described as mid-crop mortality syndrome and in healthy shrimp from australia, tha ...200818768192
the gene encoding the nucleocapsid protein of gill-associated nidovirus of penaeus monodon prawns is located upstream of the glycoprotein gene.the orf2 gene of gill-associated virus (gav) of penaeus monodon prawns resides 93 nucleotides downstream of the orf1a-orf1b gene and encodes a 144-amino-acid hydrophilic polypeptide (15,998 da; pi, 9.75) containing 20 basic (14%) and 13 acidic (9%) residues and 19 prolines (13%). antiserum to a synthetic orf2 peptide or an escherichia coli-expressed glutathione s-transferase-orf2 fusion protein detected a 20-kda protein in infected lymphoid organ and gill tissues in western blots. the gav orf2 f ...200415280504
development of a monoclonal antibody specific to yellow head virus (yhv) from penaeus monodon.a monoclonal antibody specific to yellow head virus (yhv) was produced from a mouse immunized with gill extracts prepared from laboratory-reared penaeus monodon dually infected with yhv and white spot syndrome virus (wssv). one clone designated v3-2b specifically bound to native and sds-treated viral specific antigens. immunocytochemical studies of infected gills revealed viral specific immunoreactivities in the cytoplasm of gill tissue and in haemocytes. no antibody binding was observed in gill ...200010986642
histopathology of cultured shrimp showing gross signs of yellow head syndrome and white spot syndrome during 1994 indian epizootics.two epizootics of cultured shrimp occurred in india in 1994. the first case of mass mortalities of tiger shrimp penaeus monodon in july 1994 was very similar to that caused by yellowhead virus (yhv) infection with regard to gross clinical signs, host species and size of shrimp affected, but it was histologically atypical. very interestingly, intranuclear inclusions typical of a white spot syndrome virus (wssv) infection were present in shrimp showing gross signs of yellow head syndrome, suggesti ...19989789974
identification of genes expressed in response to yellow head virus infection in the black tiger shrimp, penaeus monodon, by suppression subtractive hybridization.suppression subtractive hybridization (ssh) was employed to identify yellow head virus (yhv)-responsive genes from the hemocytes of the black tiger shrimp, penaeus monodon. two ssh cdna libraries were constructed to identify viral responsive genes in the early (24i) and late (48/72i) phases of yhv infection. from 240 randomly selected clones from each library, 155 and 30 non-redundant transcripts were obtained for the early and late libraries, respectively. from these clones, 72 and 16, respecti ...201020067803
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