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genetic relatedness and phenotypic characteristics of treponema associated with human periodontal tissues and ruminant foot disease.treponema have been implicated recently in the pathogenesis of digital dermatitis (dd) and contagious ovine digital dermatitis (codd) that are infectious diseases of bovine and ovine foot tissues, respectively. previous analyses of treponemal 16s rdna sequences, pcr-amplified directly from dd or codd lesions, have suggested relatedness of animal treponema to some human oral treponema species isolated from periodontal tissues. in this study a range of adhesion and virulence-related properties of ...200312724370
activation of the classical and alternative pathways of complement by treponema pallidum subsp. pallidum and treponema vincentii.both in vivo and in vitro studies have indicated that complement plays an important role in the syphilitic immune responses. few quantitative data are available concerning activation of the classical pathway by treponema pallidum subsp. pallidum, and no information is available on treponemal activation of the alternative pathway. activation of both pathways was compared by using t. pallidum subsp. pallidum and the nonpathogen t. vincentii. with rabbit and human sources of complement, both organi ...19873305362
effects of the inorganic salts sodium chloride, sodium bicarbonate, and magnesium sulfate upon the growth and motility of treponema vincentii.the use of inorganic salt solutions as chemotherapeutic agents in the control of periodontal disease has received considerable attention in the past few years. although some research has been published on their clinical effectiveness, little is known about their therapeutic activity and bactericidal effects upon oral spirochetes. the present study investigated the effects of varied concentrations of nacl, nahco3, and mgso4 upon the in vitro growth and motility of treponema vincentii. growth dete ...19863007725
genetic characterization of an oral treponeme isolated from human subgingival plaque.we examined the genetic characteristics of a human oral treponeme isolated from subgingival plaque of a patient with periodontal disease that could not be assigned to any of the previously described oral treponema species. the guanine-plus-cytosine content of its dna was 51 mole%. the levels of dna-dna relatedness of the organism to other treponema species, including treponema denticola, treponema vincentii, treponema socranskii, treponema pallidum and treponema phagedenis, were less than 30%. t ...19979467407
treponema maltophilum sp. nov., a small oral spirochete isolated from human periodontal lesions.a novel culture medium for cultivation of fastidious oral anaerobes is described. this medium, omiz-pat, consists of a rich chemically defined basal medium supplemented with asialofetuin, as well as yeast extract and neopeptone fractions. addition of 1 mg of rifampin per liter and 100 mg of fosfomycin per liter allowed routine isolation of spirochetes by a limit dilution method in 96-well plates containing liquid omiz-pat. in addition to members of the four previously recognized species of oral ...19968782684
taxonomy and virulence of oral spirochetes.all oral spirochetes are classified in the genus treponema. this genus is in the family spirochaetaceae as in bergey's manual of systematic bacteriology. other generic members of the family include spirochaeta, cristispira and borrelia. this conventional classification is in accord with phylogenetic analysis of the spirochetes based on 16s rrna cataloguing. the oral spirochetes fall naturally within the grouping of treponema. only four species of treponema have been cultivated and maintained rel ...200011155157
a spirochete isolated from a case of severe virulent ovine foot disease is closely related to a treponeme isolated from human periodontitis and bovine digital dermatitis.the isolation of spirochetes from severe ovine foot disease has been reported recently by our research group. in this study we describe the preliminary classification of this spirochete based on nucleotide sequence analysis of the pcr-amplified 16s rrna gene. phylogenetic analysis of this sequence in comparison with other previously reported 16s rrna gene sequences showed that the spirochete belonged to the treponemal phylotype treponema vincentii which has been associated with bovine digital de ...200010808093
restriction fragment-length polymorphism analysis of 16s ribosomal rna genes amplified by polymerase chain reaction for rapid identification of cultivable oral treponemes.although oral treponemes are among the most frequently found bacteria in periodontal pockets, identification of these organisms can be difficult. in this study, restriction fragment-length polymorphism (rflp) analysis of polymerase chain reaction (pcr)-amplified 16s ribosomal rna genes (16s rrna gene pcr-rflp) was used to generate restriction profiles of reference strains of oral treponemes including treponema denticola, treponema socranskii, treponema vincentii. treponema pectinovorum and trepo ...199910219171
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