autolysis of lactococcus lactis caused by induced overproduction of its major autolysin, acma.the optical density of a culture of lactococcus lactis mg1363 was reduced more than 60% during prolonged stationary phase. reduction in optical density (autolysis) was almost absent in a culture of an isogenic mutant containing a deletion in the major autolysin gene, acma. an acma mutant carrying multiple coples of a plasmid encoding acma lysed to a greater extent than the wild-type strain did. intercellular action of acma was shown by mixing end-exponential-phase cultures of an acma deletion mu ...19979212419
characterization of the putative replisome organizer of the lactococcal bacteriophage r1t.analysis of the nucleotide sequence of the genome of the lactococcal bacteriophage r1t showed that it may encode at least two proteins involved in dna replication. on the basis of its similarity with the g38p protein encoded by the bacillus subtilis phage spp1, the product of orf11 (pro11) is thought to be involved in the initiation of phage dna replication. this protein was overexpressed in lactococcus lactis and partially purified. gel retardation analysis using various r1t dna fragments indic ...200212239299
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