| the haemagglutinin-neuraminidase glycoprotein of the porcine paramyxovirus lpmv: comparison with other paramyxoviruses revealed the closest relationship to simian virus 5 and mumps virus. | the complete nucleotide sequence of the haemagglutinin-neuraminidase (hn) gene of the porcine paramyxovirus lpmv, was determined from cdna derived from viral genomic rna. the gene was 1906 nucleotides long including a putative gene end and poly a signal. one long open reading frame was found encoding a protein of 576 amino acids with a calculated molecular weight of 63,324. the protein contains four potential n-glycosylation sites and a major hydrophobic region near the n-terminal, suggesting a ... | 1992 | 1731697 |
| the structural proteins of a porcine paramyxovirus (lpmv). | the porcine paramyxovirus is a newly identified agent of a fatal disease in piglets, endemic in mexico since 1980, where it was seen around the town of la piedad, michoacan, mexico (hence lpm virus). at least six [35s]methionine-labelled proteins could be resolved by sds-page and five of them were clearly immunoprecipitated. selective labelling of lpmv-infected cells with [3h]glucosamine revealed two bands with an mr of about 66k and 59k, corresponding to the two viral glycoproteins, the haemagg ... | 1990 | 2313267 |
| analysis of the fusion protein gene of the porcine rubulavirus lpmv: comparative analysis of paramyxovirus f proteins. | complementary dna clones representing the fusion (f) protein gene of the porcine rubulavirus lpmv were isolated and sequenced. the f gene was found to be 1,845 nucleotides long containing one long open reading frame capable of encoding a protein of 541 amino acids. the cleavage motif for f0 into f1 and f2 is his-arg-lys-lys-arg. a sequence comparison and a phylogenetic analysis was performed in order to identify possible functional domains of paramyxovirus fusion proteins and also to classify th ... | 1997 | 9208455 |
| multiple factors including subgenomic rnas and reduced viral protein expression are associated with a persistent infection by porcine rubulavirus (lpmv). | the synthesis of virus specific rna and the expression of viral proteins in pk-15 cells persistently infected with the porcine rubulavirus lpmv have been studied at two different cell-passages following establishment of persistency (passages 25 and 65). protein analysis of persistently infected cells and the virus particles released from these failed to demonstrate the presence of the polymerase (l) protein. a decrease in the amount of the phospho- (p) protein was also noted. the genome and mrna ... | 1998 | 9572545 |
| immunity to porcine rubulavirus infection in adult swine. | the immune response against the porcine rubulavirus was analyzed in experimentally infected adult pigs. high titers of virus neutralizing and hemagglutinating inhibitory antibodies were identified in infected animals. the antibody specificity was directed towards hn, m, and np rubula virion proteins; immunodominance of hn proteins was demonstrated. peripheral blood mononuclear cells from infected, but not from non-infected pigs proliferated in vitro in response to virus antigenic stimuli, showin ... | 1998 | 9764728 |
| purification and characterization of the hemagglutinin-neuraminidase of porcine rubulavirus lpmv. | the hemagglutinin-neuraminidase (hn) from the lpmv strain of porcine rubulavirus was purified from virions by ultracentrifugation in a continuous 20-60% sucrose gradient and by ion exchange chromatography. the hn is a glycoprotein of 66 kda constituted by 50.5, 13.3 and 13.6% of non polar, uncharged polar, and charged polar amino acids, respectively. the hn contains 4% of carbohydrates, its glycannic portion is constituted by man, gal, glcnac, galnac, and neu5ac in 3:3:4:1:1 molar ratios. the hn ... | 1999 | 10815988 |
| identification of potential b cell epitope determinants by computer techniques, in hemagglutinin-neuraminidase from the porcine rubulavirus la piedad michoacan. | hemagglutinin-neuraminidase (hn) from porcine rubulavirus la piedad michoacan (rvplpm) is one of the most antigenic proteins known, and is responsible for virus-host cell interaction. we analyzed the amino acid sequence of hn, using computer-assisted techniques to identify b cell epitopes. from a pool of 18 possible antigenic peptides, we evaluated the antigenicity of the 2 peptides with the highest scores and the 1 with lowest score. antibodies from rvplpm-infected pigs recognized the synthesiz ... | 2007 | 17603842 |
| investigation of t-cell responses and viral mrna persistence in lymph nodes of pigs infected with porcine rubulavirus. | selected lymphocyte subpopulations were studied and the distribution of viral mrna were investigated during acute and persistent porcine rubulavirus (porv-lpmv) infection in vietnamese pot-bellied pigs. six pigs infected with porv-lpmv at 17 days of age exhibited clinical signs 7-10 days post-inoculation (pi). one infected piglet died 11 days pi while the other five recovered around day 13 pi and survived until euthanasia on day 277 pi. increased numbers of cd8+, cd4+ and cd2+ t cells were detec ... | 2009 | 18986711 |
| multiplex pcr and multiplex rt-pcr for inclusive detection of major swine dna and rna viruses in pigs with multiple infections. | multiplex pcr and multiplex rt-pcr were developed to identify nine viruses in pigs with multiple infections. these viruses are: porcine circovirus type 2 (pcv2), suid herpesvirus 1, porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus (prrsv), japanese encephalitis virus, porcine rotavirus a (porv-a), porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and getah virus. these methods were shown to be high specificity and sensitivity. in the ... | 2009 | 19467264 |
| identification of antigenic variants of the porcine rubulavirus in sera of field swine and their seroprevalence. | we sampled sera from 1013 non-vaccinated swine from four states in mexico, guanajuato, jalisco, michoacán and the estado de mexico, to analyse anti-porcine rubulavirus antibody titres against three different porcine rubulavirus isolates (pac-4/1993, pac-6/2001, and pac-9/2003) using a hemagglutination inhibition assay. the results revealed that there were antigenic differences among the isolates assessed. in particular, the estimated correlation between the pac-4/1993 and pac-6/2001 (0.50) isola ... | 2011 | 22171991 |
| host and viral traits predict zoonotic spillover from mammals. | the majority of human emerging infectious diseases are zoonotic, with viruses that originate in wild mammals of particular concern (for example, hiv, ebola and sars). understanding patterns of viral diversity in wildlife and determinants of successful cross-species transmission, or spillover, are therefore key goals for pandemic surveillance programs. however, few analytical tools exist to identify which host species are likely to harbour the next human virus, or which viruses can cross species ... | 2017 | 28636590 |
| molecular characterization of the hemagglutinin-neuraminidase gene of porcine rubulavirus isolates associated with neurological disorders in fattening and adult pigs. | "blue eye disease" is a viral infection of swine endemic in mexico, which produces fatal encephalitis accompanied by respiratory signs and corneal opacity in suckling piglets. an atypical blue eye disease outbreak presented high rates of neurological signs in fattening and adult pigs from 2000 to 2003. in order to identify the basis of increased neurovirulence, the hemagglutinin-neuraminidase (hn) gene of several porcine rubulavirus isolates were sequenced and compared with that of la piedad mic ... | 2008 | 18031776 |
| full-length genome sequence and genetic relationship of two paramyxoviruses isolated from bat and pigs in the americas. | mapuera virus (mprv) was isolated from a fruit bat in brazil in 1979, but its host range and disease-causing potential are unknown. porcine rubulavirus (porv) was identified as the aetiological agent of disease outbreaks in pigs in mexico during early 1980s, but the origin of porv remains elusive. in this study, the completed genome sequence of mprv was determined, and the complete genome sequence of porv was assembled from previously published protein-coding genes and the non-coding genome regi ... | 2007 | 17385069 |
| semen alterations in porcine rubulavirus-infected boars are related to viral excretion and have implications for artificial insemination. | porcine rubulavirus (porv), also known as blue eye disease (bed) of swine, causes respiratory and reproductive problems in pigs at several developmental stages. to study the effect of porv infection on semen production, five boars were infected with 1 x 10(6) tcid(50)/ml of porv strain pac-3 and evaluated for 59 days post inoculation (dpi). infected boars developed reproductive tract pathology that included swelling of the testes and epididymides. analysis of the semen showed that the infection ... | 2007 | 17368687 |
| blue eye disease porcine rubulavirus (porv) infects pig neurons and glial cells using sialo-glycoprotein as receptor. | pig neural cells express glycoproteins with sialylated n-linked oligosaccharide chains (snoc) which are used by the porcine rubulavirus (porv) as receptors. pig neuronal or glial cell cultures were employed to investigate (a) whether porv infects such cells using a molecule expressing snoc, and (b) the role of viral envelope glycoproteins in establishing the infection. enriched neuronal or glial cell cultures were exposed to porv and infection was detected immunocytochemically. neuronal cultures ... | 2007 | 16459111 |
| purification of the porcine rubulavirus attachment protein by liquid isoelectric focusing. | porcine rubulavirus (porv) is an emerging virus responsible for meningoencephalitis, respiratory distress, and reproductive alterations in pigs. the hemagglutinin-neuraminidase (hn) glycoprotein is the most exposed and antigenic of the virus proteins. hn plays central roles in porv infection; i.e., it recognizes sialic acid-containing cell receptors that mediate virus attachment and penetration; in addition, its neuraminidase (sialic acid hydrolysis) activity has been proposed to be a virulence ... | 2004 | 15039074 |
| experimental porcine rubulavirus (la piedad-michoacan virus) infection in pregnant gilts. | porcine rubulavirus (la piedad-michoacan virus) (porv-lpmv) is a member of the paramyxoviridae family that causes encephalitis in young piglets and infertility in adult sows and boars. infertility in sows naturally infected by porv-lpmv is characterized by an increased number of returns to oestrus, stillbirths and mummified fetuses. in this study, nine seronegative gilts were inoculated intranasally with the pac-3 strain of porv-lpmv at week 6 or 10 of gestation. these animals were then killed a ... | 2004 | 14693118 |
| apoptosis in lymph nodes and changes in lymphocyte subpopulations in peripheral blood of pigs infected with porcine rubulavirus. | in a first experiment, five pigs were inoculated intranasally with porcine rubulavirus (porv) at 5 days of age and killed 7 days post-infection (pi). in a second experiment, four pigs were infected with the same virus at 17 days of age and killed at 9 or 15 days pi. control piglets in each experiment received uninfected cell culture supernate. all porv-infected pigs developed respiratory and nervous signs, and histological lesions of non-suppurative encephalitis and interstitial pneumonia. all c ... | 2003 | 12531681 |
| both the p and v proteins of the porcine rubulavirus lpmv interact with the np protein via their respective c-terminal unique parts. | in this paper we show that the porcine rubulavirus lpmv phosphoprotein (p) and v protein (v) both interact with the nucleoprotein (np). there are also indications for an interaction between p and v with l protein. further analysis of the domains of the p and v which are necessary for interaction with the np protein demonstrates that the interaction is not mediated from their common part but instead from their unique c-terminal parts, respectively. the common n-terminus of p and v appear to media ... | 2002 | 11864739 |
| peanut and amaranthus leucocarpus lectins discriminate between memory and naive/quiescent porcine lymphocytes. | lectins are relevant tools to isolate and characterize different cellular sub-populations. in this work, we used the lectins arachis hypogaea (peanut agglutinin, pna) and amaranthus leucocarpus (all), specific for galss1, 3galnac, to characterize naive and memory lymphocytes from pigs, experimentally infected with the porcine rubulavirus (rvp). our results showed that both lectins recognized preferentially lymphocytes with the cd4(+)cd8(+) phenotype (p<0.05). the phenotypic analysis of the cells ... | 2002 | 11825599 |
| uptake of porcine rubulavirus (lpmv) by pk-15 cells. | the porcine virus denominated la piedad michoacan virus (lpmv) is a member of the family paramyxoviridae and is the cause of a disease in pigs present only in mexico. the disease is characterized by meningoencephalitis and respiratory distress in young pigs, epididymitis and orchitis in boars, and reproductive failure and abortion in sows. | 2001 | 11578755 |
| comparative evaluation of the cd4+cd8+ and cd4+cd8- lymphocytes in the immune response to porcine rubulavirus. | the porcine immune system is unique in the expression of cd4+cd8+ (double-positive, dp) lymphocytes. these cells have been associated with immunological memory due to their gradual increase with age, the expression of memory phenotype and their ability to respond to recall viral antigen. this work analyzes the biological function of cd4+cd8- and cd4+cd8+ lymphocytes in the immune response to porcine rubulavirus (prv). cd4+cd8- cells isolated from pigs 3 weeks after infection with porcine rubulav ... | 2001 | 11389959 |
| development of a blocking elisa for screening antibodies to porcine rubulavirus, la piedad michoacan virus. | a blocking enzyme-linked immunosorbent assay (elisa) was developed to detect antibodies to porcine rubulavirus (la piedad michoacan virus [lpmv]) in serum samples from pigs. the test, based on a monoclonal antibody against the lpmv hemagglutinin-neuraminidase glycoprotein, had a sensitivity of 99% and a specificity of 97%. the results of this test were in agreement with those obtained by an indirect elisa and hemagglutination inhibition, indirect immunofluorescence, and virus neutralization test ... | 1999 | 10424646 |
| porcine rubulavirus lpmv rna persists in the central nervous system of pigs after recovery from acute infection. | in order to study persistence of the porcine rubulavirus lpmv, we examined tissue samples collected from pigs 53 days after experimental infection. these pigs survived the initial infection and could clinically be considered to have recovered from the infection. two of the pigs used in this study were chemically immunosuppressed during the last 4 days before necropsy. no infectious virus or viral antigen could be detected in any tissue using standard methods for virus isolation and detection. ho ... | 1998 | 9839652 |
| secondary structure prediction of the hemagglutinin-neuraminidase from a porcine rubulavirus. | the hemagglutinin-neuraminidase (hn) from 'la piedad, michoacan' porcine rubulavirus (lpmv) interacts specifically with neuac alpha 2,3 lactose residues on the target cell. in this work we report the secondary structure of this protein, determined with five different theoretical algorithms. results indicate that the hn protein is organized in: an intracellular region (from amino acid 1 to 25); in a beta-strand transmembrane region (residue 26 to 47), typically hydrophobic, rigid and solvent inac ... | 1998 | 9541617 |
| lesions in the reproductive tract of boars experimentally infected with porcine rubulavirus. | "blue eye" disease of pigs in mexico is caused by porcine rubulavirus and characterized by infertility in sows and boars, nervous signs in young pigs, and corneal opacity in pigs of all ages. the pathogenesis of reproductive tract lesions in rubulavirus-infected boars has not previously been investigated. in a first experiment, four 9-month-old boars were inoculated with porcine rubulavirus and killed 5, 15, 30 or 45 days post-inoculation (pi). in a second experiment, four similar boars were ino ... | 1997 | 9447484 |
| establishment and characterisation of a porcine rubulavirus (lpmv) persistent infection in porcine kidney cells. | porcine rubulavirus (lpmv) can establish persistent infections in porcine kidney cells. cell cultures characterised at passages 25 and 65 demonstrated haemadsorption, formation of syncytia, and a slower growth rate. the nucleoprotein (np) and haemagglutinin-neuraminidase (hn) protein were present in all cells, although not to the same extent as in wild type infected cells. incubation of the cell cultures with virus neutralising antibodies could not cure them from the infection. the cells were re ... | 1997 | 9444775 |
| neuac alpha 2,3gal-glycoconjugate expression determines cell susceptibility to the porcine rubulavirus lpmv. | relevance of membrane sialoglycoconjugates as receptors for infection by the porcine rubulavirus has been determined in vitro by sugar and lectin competition assays and by inhibition of glycosylation. our results show that neuac alpha 2,3gal but not neuac alpha 2,6gal inhibits the virus infectivity of vero cells, and the virus was effectively blocked with the lectin maackia amurensis, specific for neuac alpha 2,3gal. inhibition of the cellular glycosylation with tunicamycin, deoxinojirimycin as ... | 1997 | 9440225 |
| analysis of the large (l) protein gene of the porcine rubulavirus lpmv: identification of possible functional domains. | the complete nucleotide sequence of the porcine rubulavirus lpmv (la piedad michoacan virus) large (l) protein gene was determined and analysed. the l mrna was found to span 6,786 nucleotides, containing one single large open reading frame (orf), putatively encoding a polypeptide of 2,251 amino acids. by aligning the amino acid sequence of the lpmv l-protein with l-protein of a number of viruses belonging to the order mononegavirale, a high degree of similarity between the lpmv l-protein and oth ... | 1997 | 9140194 |
| a comparative study on the use of virus and antibody detection techniques for the diagnosis of la piedad michoacan paramyxovirus (lpmv) infection in pigs. | la piedad michoacan paramyxovirus (lpmv) is newly recognized paramyxovirus that has been associated with neurologic and reproductive disorders in pigs in mexico. to date, no comparative study of methods for the diagnosis of infection with this virus has been published. in this study, we identified tissues containing maximum virus load to optimize virus isolation procedures, and we compared this method to a rapid diagnostic test employing immunostaining of impression smears for lpmv antigens. in ... | 1997 | 9087918 |
| a sequential study of experimental porcine paramyxovirus (lpmv) infection of pigs: immunostaining of cryostat sections and virus isolation. | la piedad michoacan paramyxovirus (lpmv) is a recently recognized paramyxovirus infecting pigs throughout mexico. disease syndromes observed in field cases associated with lpmv infection include neurologic, respiratory, and reproductive disorders. clinical signs and the distribution of lpmv virus and antigen in tissue samples from pigs experimentally infected with lpmv by natural routes were studied. severe neurologic disease and death occurred following experimental inoculation of 3- and 17-day ... | 1996 | 8953523 |
| identification of the porcine paramyxovirus lpmv matrix protein gene: comparative sequence analysis with other paramyxoviruses. | the complete nucleotide sequence of the gene encoding the matrix protein (m) of the porcine paramyxovirus lpmv has been determined. the gene is 1376 nucleotides long including 5' and 3' non-coding sequences with a protein-coding sequence of 1107 nucleotides. the deduced protein, containing 369 amino acids with a calculated mr of 41,657, is hydrophobic overall with a net positive charge of +17.5. comparative sequence analysis revealed high amino acid homology to other paramyxovirus m proteins, wi ... | 1991 | 2033388 |
| the p gene of the porcine paramyxovirus lpmv encodes three possible polypeptides p, v and c: the p protein mrna is edited. | the nucleotide sequence of the p gene of the porcine paramyxovirus la-piedad-michoacan-mexico virus (lpmv) was analysed. three long open reading frames (orfs) were found in the mrna sense. insertion of two g residues is necessary to obtain an orf encoding the p protein, which gives a p protein of 404 amino acids with a calculated mr of 42475. this form of editing was demonstrated, two non-templated g residues being added in a portion of the mrna transcripts. the lpmv v protein, which has a conse ... | 1992 | 1588322 |
| the molecular biology of the porcine paramyxovirus lpmv. | protein and genomic studies of a previously uncharacterized porcine paramyxovirus (designated lpmv) confirmed that it was a member of the paramyxovirus genus. the nucleotide sequences and deduced amino acids of the complete p-gene, m-gene, f-gene and hn-gene as well as the intergenic sequences have been determined. comparative sequence analysis of the m-gene of lpmv revealed the closest relationship of lpmv was to human mumps virus with a homology of 46% and 55% at the amino acid and nucleotide ... | 1992 | 1481359 |
| [biological characterization of three natural isolates of the porcine rubulavirus (mexico)]. | biological characterization of three natural isolates of the porcine rubulavirus (mexico). porcine rubulavirus (porv) produces a neurological and reproductive syndrome in pigs called the blue-eye disease, known only from mexico. several isolates were grouped by the main symptoms presented during outbreaks: a) neurotropic in piglets, (b) broadly neurotropic in piglets and gonadotropic in adults, and (c) gonadotropic in adults. we studied some biological properties of three strains, which fall in ... | 2008 | 19256422 |