independence of bacteriophage n15 lytic and linear plasmid replication from the heat shock proteins dnaj, dnak, and grpe.the chromosome of the temperate bacteriophage n15 replicates as a linear plasmid with covalently closed ends (or hairpins) when it forms a lysogen. i found that, in contrast to the cases for lambda and the low-copy-number plasmids f and p1, both phage and plasmid replication of n15 are independent of the heat shock proteins dnaj, dnak, and grpe.19911917885
[structural organization and control of expression of the sop-operon of linear plasmid prophage n15].stable inheritance of bacterial chromosomes and low-copy-number plasmids depends on the active partition of replicated molecules between daughter cells. the partition mechanism is well known for circular plasmids f and p1. the mechanism of partition of linear replicons was studied with the example of bacteriophage n15, which persists as a linear plasmid with covalently closed ends on lysogeny, rather than integrating into the escherichia coli chromosome. since stable inheritance of n15 is due to ...200415125235
repa protein of the bacteriophage n15 exhibits activity of dna helicase. 200415523829
functional characterization of the repa replication gene of linear plasmid prophage n15.the prophage of coliphage n15 is not integrated into the chromosome, but exists as a linear plasmid molecule with covalently closed ends. the only phage gene required for replication of circular n15 miniplasmids is repa (gene 37). here we show that repa-driven replication of the n15-based circular and linear miniplasmids is independent of host dnab helicase protein, but requires the host dnag primase. replication of phage n15 dna during lytic growth following infection does not depend on either ...200616129583
protelomerase uses a topoisomerase ib/y-recombinase type mechanism to generate dna hairpin ends.protelomerases are enzymes responsible for the generation of closed hairpin ends in linear dna. it is proposed that they use a breaking-and-rejoin type mechanism to affect dna rearrangement on specific dna sequences. in doing so, one strand turns around and becomes the complementary strand. using the purified enzyme from the escherichia coli phage n15 and the klebsiella phage phiko2 and synthetic oligonucleotide substrates, we directly demonstrate the location where the cutting/re-ligation occur ...200415001353
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