| production of digoxigenin-labelled dna probe for detection of muscovy duck parvovirus. | a chemiluminescent dot-blot hybridization assay was developed for the detection of muscovy duck parvovirus (dpv) by using a non-radioactive dpv dna probe. a 1030bp hindiii-bg/ii fragment of dpv dna was labelled with digoxigenin-labelled dutp. the hybridized dpv dna probes were detected by an immunoenzymatic reaction using anti-digoxigenin-antibody fab fragments conjugated to alkaline phosphatase and visualized by chemiluminescent reaction. the assay proved to be sensitive since up to 3 fg of hom ... | 1995 | 7760858 |
| development and evaluation of an immunochromatographic strip test based on the recombinant ul51 protein for detecting antibody against duck enteritis virus. | duck enteritis virus (dev) infection causes substantial economic losses to the worldwide duck-producing areas. the monitoring of dev-specific antibodies is a key to evaluate the effect of dev vaccine and develop rational immunization programs. thus, in this study, an immunochromatographic strip (ics) test was developed for detecting dev serum antibodies. | 2010 | 20946624 |
| the development of a rapid sybr green i-based quantitative pcr for detection of duck circovirus. | this report describes a one-step real-time polymerase chain reaction assay based on sybr green i for detection of a broad range of duck circovirus (ducv). align with all ducv complete genome sequences and other genus circovirus download from the genbank (such as goose circovirus, pigeon circovirus), the primers targets to the replicate gene of ducv were designed. the detection assay was linear in the range of 1.31 × 102-1.31 × 107 copies/μl. the reaction efficiency of the assay using the slope ( ... | 2011 | 21978576 |
| characteristics of the genome of goose parvovirus. | the nucleic acid of goose parvovirus showed sensitivity to dnase and mung bean nuclease treatment and resistance to digestion with rnase. viral dna readily served as a template for self-primed conversion in vitro into a double-stranded form of about 5000 base pairs. there was evidence for encapsidation of strands of opposite polarities in equal amounts. the restriction enzyme cleavage patterns of goose and muscovy duck parvovirus dnas differed significantly. however, they showed a high degree of ... | 1994 | 18671101 |
| isolation and molecular characterization of a new muscovy duck parvovirus from muscovy ducks in the usa. | between 1997 and 1999 several cases of a new disease in muscovy ducks were reported in pennsylvania, usa. the cases were characterized by locomotor dysfunction, weakness, recumbency, 40 to 60% morbidity and 10 to 40% mortality. the most characteristic microscopic lesions were moderate to severe degenerative rhabodomyopathy. in order to characterize the aetiological agent, virus isolation was attempted from the spleen, liver, heart, skeletal muscle and intestine by inoculation of 14-day-old musco ... | 2006 | 17121731 |
| expression of capsid proteins and non-structural proteins of waterfowl parvoviruses in escherichia coli and their use in serological assays. | while there are a number of methods available for detection of antibodies against waterfowl parvoviruses, none is able to differentiate responses against the capsid and non-structural proteins. to enable this, the capsid and non-structural proteins of goose parvovirus (gpv) and muscovy duck parvovirus (mdpv) were expressed in escherichia coli. these proteins were purified and used as antigens in western blotting assays of antibodies against gpv and mdpv. the results showed that 94.7% of the goos ... | 2005 | 16236567 |
| genetic variation of viral protein 1 genes of field strains of waterfowl parvoviruses and their attenuated derivatives. | to understand the genetic variations between the field strains of waterfowl parvoviruses and their attenuated derivatives, we analyzed the complete nucleotide sequences of the viral protein 1 (vp1) genes of nine field strains and two vaccine strains of waterfowl parvoviruses. sequence comparison of the vp1 proteins showed that these viruses could be divided into goose parvovirus (gpv) related and muscovy duck parvovirus (mdpv) related groups. the amino acid difference between gpv- and mdpv-relat ... | 2004 | 15529973 |
| identification of a recombinant muscovy duck parvovirus (mdpv) in shanghai, china. | the full-length genome of strain saas-shnh, a mdpv isolated from muscovy duck in shanghai, has been sequenced and shown to share 93.7% nucleotide identity with mdpv strain fm (nc_006147). two putative genetic recombination events were identified as occurring within the 419-610 nt and 3113-4241 nt regions of the saas-shnh genome which, for the first time, provide evidence of recombination between mdpvs and gpvs. | 2014 | 25465183 |
| development of an indirect elisa with epitope on nonstructural protein of muscovy duck parvovirus for differentiating between infected and vaccinated muscovy ducks. | the aim of this study was to develop an indirect enzyme-linked immunosorbent assay (i-elisa) based on epitope aa503-509 (ranepke), which is on nonstructural protein of muscovy duck parvovirus (mdpv). sera (100) from negative and vaccinated muscovy ducks were compared with infected sera (240) to establish the cut-off value of this i-elisa. there was a significant difference between the positive and negative populations (p < 0·05). the adoption of this positive-negative threshold value for this i- ... | 2014 | 25163937 |
| immunologic cross-reactivity between muscovy duck parvovirus and goose parvovirus on the basis of epitope prediction. | through bioinformatic prediction, between muscovy duck parvovirus (mdpv) and goose parvovirus (gpv), there were one epitope aa503-509 (ranepke) on non-structural protein and three epitopes aa426-430 (sqdld), 540-544 (dpyrs), 685-691 (kenskrw) on structural protein might cross-react with each other. furthermore, the four epitops were expressed in escherichia coli. all the four recombinant proteins could react with gpv-antisera and mdpv-antisera in western blot. | 2013 | 24294250 |
| evidence of muscovy duck parvovirus in muscovy ducklings in california. | muscovy duck parvovirus (mdpv) has been demonstrated in tissue samples from one- to four-week-old commercially reared muscovy ducks that were weak, unable to walk and had a high mortality rate. on postmortem examination, the thigh and leg muscles, and the myocardium were found to be pale, and there was a fibrinous exudate on the capsule of the liver, and ascites. the parvovirus was isolated in embryonated muscovy duck eggs and visualised by negative stain electron microscopy, detected by polymer ... | 2000 | 10674693 |
| detection of goose and muscovy duck parvoviruses using polymerase chain reaction-restriction enzyme fragment length polymorphism analysis. | by using primers (al18f2 and al18r2) designed from goose parvovirus (gpv) strain ihc, an 806-bp band was amplified by polymerase chain reaction (pcr) from all of 17 samples from thailand. specificity to gpv was confirmed by southern hybridization. with restriction enzyme digestion of the pcr products, two isolates differed from the other 15 isolates by the absence of restriction sites for hincii and bglii and the presence of ecor1 site. nucleotide sequence analysis of the pcr products from the d ... | 1998 | 9533090 |
| biochemical and genomic characterization of muscovy duck parvovirus. | a duck parvovirus (dpv) isolated from muscovy ducks during the epizootic in france in 1989 was purified from inoculated allanto-amniotic fluids by cscl density gradient centrifugation and characterized. full and empty non-enveloped icosahedral viral particles were observed banding at densities of 1.39 to 1.42 and 1.38 respectively, with a diameter of 22 to 23 nm. viral proteins were analyzed by sds-page and the estimated molecular weights of the 3 major proteins were 91, 78 and 58 kda. the nucle ... | 1994 | 7826205 |
| quantitative analysis of waterfowl parvoviruses in geese and muscovy ducks by real-time polymerase chain reaction: correlation between age, clinical symptoms and dna copy number of waterfowl parvoviruses. | waterfowl parvoviruses cause serious loss in geese and ducks production. goose parvovirus (gpv) is infectious for geese and ducks while muscovy duck parvovirus (mdpv) infects muscovy ducks only. so far, for these viruses' sensitive detection polymerase chain reaction (pcr) and loop-mediated isothermal amplification (lamp) were applied. however, there was no molecular biology method for both waterfowl parvoviruses detection and quantification which could unify the laboratory procedures. the level ... | 2012 | 22420608 |
| evidence for natural recombination in the capsid gene vp2 of taiwanese goose parvovirus. | to investigate the possible role of recombination in the evolution of muscovy duck parvovirus (mdpv) and goose parvovirus (gpv) in taiwan, we analyzed a potentially significant recombination event that occurred only in gpv by comparing thirteen complete sequences of the capsid gene vp2 of gpv and mdpv. the recombination event occurred between gpv strain 06-0239 as the minor parent and strains 99-0808 as the major parent, which resulted in the gpv recombinant v325/tw03. gpv v325/tw03 is likely to ... | 2015 | 26085285 |
| identification of recombination between muscovy duck parvovirus and goose parvovirus structural protein genes. | waterfowl parvoviruses are divided into muscovy duck parvoviruses (mdpvs) and goose parvoviruses (gpvs). phylogenetic analysis based on structural gene nucleotide sequences showed that the strains of three gpvs (dy, pt and d strains) and two mdpvs (gx5 and saah-shnh) are closely related and formed one cluster. recombination analysis showed that recombination between gpv-gdfsh and mdpv-89384/france strains led to five recombinant strains: gpv-dy, gpv-pt, gpv-d, mdpv-gx5 and mdpv-saah-shnh. the re ... | 2015 | 26239342 |
| isolation and genomic characterization of a duck-origin gpv-related parvovirus from cherry valley ducklings in china. | a newly emerged duck parvovirus, which causes beak atrophy and dwarfism syndrome (bads) in cherry valley ducks, has appeared in northern china since march 2015. to explore the genetic diversity among waterfowl parvovirus isolates, the complete genome of an identified isolate designated sdlc01 was sequenced and analyzed in the present study. genomic sequence analysis showed that sdlc01 shared 90.8%-94.6% of nucleotide identity with goose parvovirus (gpv) isolates and 78.6%-81.6% of nucleotide ide ... | 2015 | 26465143 |
| phylogenetic analysis of vp1 gene sequences of waterfowl parvoviruses from the mainland of china revealed genetic diversity and recombination. | to determine the origin and evolution of goose parvovirus (gpv) and muscovy duck parvovirus (mdpv) in the mainland of china, phylogenetic and recombination analyses in the present study were performed on 32 complete vp1 gene sequences from china and other countries. based on the phylogenetic analysis of the vp1 gene, gpv strains studied here from mainland china (prc) could be divided into three genotypes, namely prc-i, prc-ii and prc-iii. genotype prc-i is indigenous to mainland china. only one ... | 2016 | 26692144 |
| identification of antigenic domains in the non-structural protein of muscovy duck parvovirus. | muscovy duck parvovirus (mdpv) infection is widespread in many muscovy-duck-farming countries, leading to a huge economic loss. by means of overlapping peptides expressed in escherichia coli in combination with western blot, antigenic domains on the non-structural protein (nsp) of mdpv were identified for the first time. on the western blot, the fragments ns(481-510), ns (501-530), ns (521-550), ns (541-570), ns (561-590), ns (581-610) and ns (601-627) were positive (the numbers in parentheses i ... | 2016 | 27154558 |
| identification of goose-origin parvovirus as a cause of newly emerging beak atrophy and dwarfism syndrome in ducklings. | a recent epizootic outbreak, in china, of duck beak atrophy and dwarfism syndrome (bads) was investigated using electron microscopic, genetic, and virological studies, which identified a parvovirus with a greater similarity to goose parvovirus (gpv) (97% protein homology) than to muscovy duck parvovirus (mdpv) (90% protein homology). the new virus, provisionally designated gpv-qh15, was found to be antigenically more closely related to gpv than to mdpv in a virus neutralization assay. these find ... | 2016 | 27194692 |
| isolation and characterization of a distinct duck-origin goose parvovirus causing an outbreak of duckling short beak and dwarfism syndrome in china. | many mule duck and cherry valley duck flocks in different duck-producing regions of china have shown signs of an apparently new disease designated "short beak and dwarfism syndrome" (sbds) since 2015. the disease is characterized by dyspraxia, weight loss, a protruding tongue, and high morbidity and low mortality rates. in order to characterize the etiological agent, a virus designated sbdsv m15 was isolated from allantoic fluid of dead embryos following serial passage in duck embryos. this viru ... | 2016 | 27314945 |
| construction and sequencing of an infectious clone of the goose embryo-adapted muscovy duck parvovirus vaccine strain fz91-30. | muscovy duck parvovirus (mdpv) is the etiological agent of muscovy duckling parvoviral disease, which is characterized by diarrhea, locomotive dysfunction, stunting, and death in young ducklings, and causes substantial economic losses in the muscovy duck industry worldwide. fz91-30 is an attenuated vaccine strain that is safe and immunogenic to ducklings, but the genomic information and molecular mechanism underlining the attenuation are not understood. | 2016 | 27329377 |
| analysis of the genome sequence of the pathogenic muscovy duck parvovirus strain yy reveals a 14-nucleotide-pair deletion in the inverted terminal repeats. | genomic information about muscovy duck parvovirus is still limited. in this study, the genome of the pathogenic mdpv strain yy was sequenced. the full-length genome of yy is 5075 nucleotides (nt) long, 57 nt shorter than that of strain fm. sequence alignment indicates that the 5' and 3' inverted terminal repeats (itr) of strain yy contain a 14-nucleotide-pair deletion in the stem of the palindromic hairpin structure in comparison to strain fm and fz91-30. the deleted region contains one "e-box" ... | 2016 | 27344160 |
| identification of recombination among vp1 gene of muscovy duck parvovirus from the mainland of china. | | 2016 | 27771073 |
| analysis of evolutionary processes of species jump in waterfowl parvovirus. | waterfowl parvoviruses are classified into goose parvovirus (gpv) and muscovy duck parvovirus (mdpv) according to their antigenic features and host preferences. a novel duck parvovirus (ndpv), identified as a new variant of gpv, is currently infecting ducks, thus causing considerable economic loss. this study analyzed the molecular evolution and population dynamics of the emerging parvovirus capsid gene to investigate the evolutionary processes concerning the host shift of ndpv. two important am ... | 2017 | 28352261 |
| a one-step duplex rrt-pcr assay for the simultaneous detection of duck hepatitis a virus genotypes 1 and 3. | duck hepatitis a virus (dhav) is a highly infectious pathogen that causes significant bleeding lesions in the viscera of ducklings less than 3 weeks old. there are three serotypes of dhav: serotype 1 (dhav-1), serotype 2 (dhav-2) and serotype 3 (dhav-3). these serotypes have no cross-antigenicity with each other. to establish an rrt-pcr assay for the rapid detection of a mixed infection of dhav-1 and dhav-3, two pairs of primers and a pair of matching taqman probes were designed based on conserv ... | 2016 | 27435338 |
| development and evaluation of a vp3-elisa for the detection of goose and muscovy duck parvovirus antibodies. | the vp3-encoding gene of goose parvovirus (gpv) ep22 strain was cloned and expressed in escherichia coli. the gpv vp3-encoding gene was 1605 bp in length, and it encoded a 534 amino acid protein with a predicted molecular mass of 59.9 kda. the vp3 fusion protein expressed in e. coli was detected by goose and muscovy duck anti-parvovirus polyclonal sera. in addition, an elisa (vp3-elisa) using the vp3 protein as the coating antigen for the detection of antibodies to gpv in geese and antibodies to ... | 2010 | 19913055 |
| short beak and dwarfism syndrome of mule duck is caused by a distinct lineage of goose parvovirus. | from the early 1970s to the present, numerous cases of short beak and dwarfism syndrome (sbds) have been reported in mule ducks from france. the animals showed strong growth retardation with smaller beak and tarsus. it was suggested that the syndrome was caused by goose parvovirus on the basis of serological investigation, but the causative agent has not been isolated and the disease has not so far been reproduced by experimental infection. the aim of the present study was to characterize the vi ... | 2009 | 19322718 |
| phylogenetic analysis of parvoviruses isolated in taiwan from ducks and geese. | two major outbreaks of parvovirus infection occurred in domestic waterfowls in taiwan in the last two decades; the first was in 1982 and the second in 1989/1990. parvoviruses isolated in the two outbreaks were sequenced between nucleotides 142 and 680 of the vp3 gene. sequence comparisons reveal that these viruses could be divided into two groups respectively related to goose parvovirus (gpv) and muscovy duck parvovirus (mdpv). nucleotide differences between ''gpv'' and ''mdpv'' groups range fro ... | 2000 | 19184788 |
| [prokaryotic expression of vp3 gene of muscovy duck parvovirus, and its antiserum preparation for detection of virus multiplication]. | new epidemic broke out in recent year which was suspected to be caused by variant muscovy duck parvovirus (mdpv). for this reason, new mdpv detection methods are needed for the new virus strains. in this study, a pair of primers were designed according to the full-length genome of mdpv strain saas-shnh, which were identified in 2012, and were used to amplify the vp3 gene of mdpv by polymerase chain reaction. after being sequenced, the vp3 gene was subcloned into the prokaryotic expression vector ... | 2015 | 26021080 |
| construction of an infectious plasmid clone of muscovy duck parvovirus by ta cloning and creation of a partially attenuated strain. | muscovy duck parvovirus (mdpv) infection is a highly contagious and fatal disease of muscovy ducklings. the infectious clone methodology is a valuable tool to study the pathogenic mechanisms of viruses, but no infectious clone of mdpv is yet available. in this study, a plasmid clone containing the full-length genome of mdpv was constructed using the ta cloning methodology. this mdpv clone was found to be infectious after transfection of primary muscovy duck embryo fibroblast cells and passage in ... | 2015 | 25609267 |
| molecular characterization of the full muscovy duck parvovirus, isolated in guangxi, china. | we report the complete genomic sequence of the full muscovy duck parvovirus (mdpv) strain, designated gx2011-5, isolated from a muscovy duck in guangxi province, china. the complete genomic sequence was 5,132 bp in length and contained two major open reading frames encoding a 1,844-nucleotide (nt) nonstructural protein and a 2,199-nt capsid protein. comparison of the complete sequence of gx2011-5 with other published sequences of muscovy duck parvovirus revealed that this strain exhibited 90.4% ... | 2014 | 25502664 |
| complete genome sequence of goose parvovirus y strain isolated from muscovy ducks in china. | a goose parvovirus (gpv) y strain was isolated from muscovy ducks in anhui province of china. by polymerase chain reaction method, its complete genomic sequence was found to be 5,106 bp in length, consisting of 444-bp inverted terminal repeat, 1,844-bp non-structural protein and 2,199-bp capsid protein (vp) regions. then its sequence was aligned with the sequences of gpv and muscovy duck parvovirus published in the genbank using the neighbor-joining method. the phylogenetic analyses based on the ... | 2014 | 24194370 |
| genetic characterization of a potentially novel goose parvovirus circulating in muscovy duck flocks in fujian province, china. | we report a novel goose parvovirus (mdgpv/pt) isolated from an affected muscovy duck in fujian province, china. in this study, the ns1 sequence analyses indicated a close genetic relationship between mdgpv/pt and muscovy duck parvovirus (mdpv) strains, although mdgpv/dy, which was isolated from a muscovy duck in 2006 in sichuan province, could be divided into gpv-related groups. phylogenetic analysis showed that except for differences in the ns1 gene, mdgpv strains pt and dy are closely related ... | 2013 | 23563621 |
| characterization of monoclonal antibodies against waterfowl parvoviruses vp3 protein. | the vp3 protein of goose parvovirus (gpv) or muscovy duck parvovirus (mdpv), a major structural protein, can induce neutralizing antibodies in geese and ducks, but monoclonal antibodies (mabs) against vp3 protein has never been characterized. | 2012 | 23176172 |
| expression of muscovy duck parvovirus capsid proteins (vp2 and vp3) in a baculovirus expression system and demonstration of immunity induced by the recombinant proteins. | the gene encoding the muscovy duck parvovirus (dpv)-strain 89384 capsid proteins vp2 and vp3 was cloned in a baculovirus expression system and expressed in insect cells. the recombinant proteins were found to react with specific anti-dpv serum by western blotting and to be located in the nucleus of insect cells (sf9) as shown by immunofluorescence. empty virus-like particles (vlps) identical in size and appearance to dpv virions were observed by electron microscopy. the antigenicity and immunoge ... | 1996 | 8811015 |
| transfection of embryonated muscovy duck eggs with a recombinant plasmid is suitable for rescue of infectious muscovy duck parvovirus. | for members of the family parvoviridae, rescue of infectious virus from recombinant plasmid is usually done in cultured cells. in this study, the whole genome of the pathogenic muscovy duck parvovirus (mdpv) strain yy was cloned into the pbluescript ii (sk) vector, generating recombinant plasmid pyy. with the aid of a transfection reagent, pyy plasmid was inoculated into 11-day-old embryonated muscovy duck eggs via the chorioallantoic membrane route, resulting in the successful rescue of infecti ... | 2017 | 28884224 |
| a polymerase chain reaction assay for detection of virulent and attenuated strains of duck plague virus. | sequence analysis of duck plague virus (dpv) revealed that there was a 528bp (b fragment) deletion within the ul2 gene of dpv attenuated vaccine strain in comparison with field virulent strains. the finding of gene deletion provides a potential differentiation test between dpv virulent strain and attenuated strain based on their ul2 gene sizes. thus we developed a polymerase chain reaction (pcr) assay targeting to the dpv ul2 gene for simultaneous detection of dpv virulent strain and attenuated ... | 2017 | 28860100 |
| genomic and pathogenic analysis of a muscovy duck parvovirus strain causing short beak and dwarfism syndrome without tongue protrusion. | in 2008, clinical cases of short beak and dwarfism syndrome (sbds) caused by muscovy duck parvovirus (mdpv) infection were found in mule duck and taiwan white duck farms in fujian, china. a mdpv lh strain causing duck sbds without tongue protrusion was isolated in this study. phylogenetic analysis show that the mdpv lh strain was clustered together with other mdpv strains, but divergent from gpv isolates. two major fragment deletions were found in the inverted terminal repeats (itr) of mdpv lh s ... | 2017 | 28715672 |
| a multiplex pcr for detection of six viruses in ducks. | in this study, six pairs of specific primers that can amplify dna fragments of different sizes were designed and synthesized according to viral protein gene sequences published in genbank. then, a multiplex pcr method was established for rapid detection of duck hepatitis virus 1, duck plague virus, duck tembusu virus, muscovy duck parvovirus, muscovy duck reovirus, and duck h9n2 avian influenza virus, and achieve simple and rapid detection of viral diseases in ducks. single pcr was used to confi ... | 2017 | 28728835 |
| genetic variance of derzsy's disease strains isolated in poland. | the aim of this study was the assessment of the genetic variance of derzy's disease (gpv) strains isolated from cases occurring in poland. the nucleotide and predicted aminoacid sequences of vp2 and vp3 surface proteins of the polish gpv strains were compared with other strains previously isolated in hungary, france, germany, china and taiwan. the observed genetic variance of the aminoacid sequence within the group of polish strains was low and reached 5% of the overall analysed sequence. consid ... | 2009 | 20076793 |
| molecular detection of muscovy duck parvovirus by loop-mediated isothermal amplification assay. | muscovy duck parvovirus (mdpv) usually causes high morbidity and mortality in 1- to 3-wk-old muscovy ducklings due to serious infections, which is an imminent threat to the commercial duck industry in china. the objectives of this study were to develop and evaluate a simple, rapid, and inexpensive loop-mediated isothermal amplification (lamp) method for specific detection of mdpv and to compare it with the pcr method in rapidity, sensitivity, and accuracy. the novel lamp assay used a set of 4 sp ... | 2010 | 20181863 |
| gexp analyzer-based multiplex reverse-transcription pcr assay for the simultaneous detection and differentiation of eleven duck viruses. | duck viral pathogens primarily include the avian influenza virus (aiv) subtypes h5, h7, and h9; duck hepatitis virus (dhv); duck tembusu virus (dtmuv); egg drop syndrome virus (edsv); duck enteritis virus (dev); newcastle disease virus (ndv); duck circovirus (ducv); muscovy duck reovirus (mdrv); and muscovy duck parvovirus (mdpv). these pathogens cause great economic losses to china's duck breeding industry. | 2015 | 26518004 |