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caprine abortion following exposure to mycoplasma capricolum subsp. capricolum. 19968953539
polymerase chain reaction and restriction endonuclease digestion for selected members of the "mycoplasma mycoides cluster" and mycoplasma putrefaciens.a specific diagnostic method using the polymerase chain reaction, together with restriction endonuclease digestion patterns, was developed for members of the "mycoplasma mycoides cluster" that normally occur in the united states (i.e., mycoplasma mycoides subsp. mycoides large colony and mycoplasma capricolum subsp. capricolum in addition to "cluster" mycoplasma, bovine serogroup 7, and mycoplasma putrefaciens. the digestion of "cluster" polymerase chain reaction dna (1,225 bp) amplification pro ...19979211239
a pathological and immunohistochemical study of goat kids undergoing septicaemic disease caused by mycoplasma capricolum subsp. capricolum, mycoplasma mycoides subsp. capri and mycoplasma mycoides subsp. mycoides (large colony type).this paper studies the pathological and immunohistochemical findings in 12 kids experimentally infected with mycoplasma capricolum subsp. capricolum (mcc), m. mycoides subsp. capri (mmc) and m. mycoides subsp. mycoides (large colony type), (mmmlc). for the demonstration of mcc, mmc and mmmlc antigens an immunoperoxidase technique based on the labelled streptavidin biotin method was used in the 12 kids inoculated with mycoplasmas and the three control kids inoculated with mycoplasma medium. all 1 ...19989588108
genetic and serological analysis of the immunogenic 67-kda lipoprotein of mycoplasma sp. bovine group 7.the gene encoding a lipoprotein of 67 kda, named p67, was cloned from mycoplasma sp. bovine group 7 strain pg50 and expressed in escherichia coli k12. analysis of the amino acid sequence derived from the dna sequence of the p67 gene revealed a typical prokaryotic signal peptidase ii membrane lipoprotein lipid attachment site and a transmembrane structure domain in the leader sequence at the amino-terminal end of the protein. protein p67 showed 91% identical amino acid residues to the lipoprotein ...19989766210
comparative analysis of the lppa locus in mycoplasma capricolum subsp. capricolum and mycoplasma capricolum subsp. capripneumoniae.the lppa gene, encoding the lipoprotein named lppa[mcaca] was characterised in mycoplasma capricolum subsp. capricolum. it encodes a lipoprotein with an apparent molecular mass of 57 kda as determined by sds-page. using antibodies directed against recombinant lppa[mcaca], we showed the expression of this lipoprotein in all m. capricolum subsp. capricolum by immunoblot analysis. the serum did not cross-react with other members of the mycoplasma mycoides cluster, hence showing that lppa[mcaca] was ...199910512041
comparison of the 16s-23s rrna intergenic spacer regions among strains of the mycoplasma mycoides cluster, and reassessment of the taxonomic position of mycoplasma sp. bovine group 7.nucleotide sequence analysis of the 16s-23s rrna intergenic spacer regions of six type or reference strains belonging to the mycoplasma mycoides cluster and of mycoplasma putrefaciens suggested the presence of two subclusters. one subcluster comprised m. mycoides subsp. mycoides small colony (sc) type, m. mycoides subsp. mycoides large colony (lc) type and m. mycoides subsp. capri, whereas the second subcluster comprised mycoplasma capricolum subsp. capricolum, m. capricolum subsp. capripneumoni ...200010843078
inactivation of mycoplasma species involved in contagious agalactia.the suitability of 5 agents for the inactivation of different field strains of the four mycoplasma species associated with contagious agalactia syndrome in goats, i.e. mycoplasma agalactiae, mycoplasma mycoides subsp. mycoides lc, mycoplasma capricolum subsp. capricolum and mycoplasma putrefaciens, was investigated. immunoprophylaxis of this syndrome is still based on inactivated vaccines, which traditionally use formalin as the inactivating agent. moreover, the limited information existing abou ...200414964115
first isolation of mycoplasma capricolum subsp. capricolum, one of the causal agents of caprine contagious agalactia, on the island of lanzarote (spain).during an unusually long period of bad weather, several outbreaks of caprine contagious agalactia (cca) were reported in a number of flocks on the island of lanzarote (canary islands, spain). clinical and subclinical mastitis in lactating goats and some cases of arthritis and pneumonia in kids were observed in the affected flocks. mycoplasma capricolum subsp. capricolum was isolated as the main causal agent of the outbreaks, associated with m. mycoides subsp. mycoides "large colony type" (mmm lc ...200716324858
the in vitro effect of six antimicrobials against mycoplasma putrefaciens, mycoplasma mycoides subsp. mycoides lc and mycoplasma capricolum subsp. capricolum isolated from sheep and goats in jordan.respiratory disease in sheep and goats is a major problem in jordan and is often associated with mycoplasma species. without effective vaccines, control is mainly by chemotherapy, but the uncontrolled use of antimicrobials has led to concerns about the potential development of antimicrobial resistance. the in vitro effect of chloramphenicol, florfenicol, enrofloxacin, tylosin, erythromycin and oxytetracycline was determined against 32 isolates of mycoplasma species-m. mycoides subsp. mycoides lc ...200617405622
a pcr for the detection of mycoplasmas belonging to the mycoplasma mycoides cluster: application to the diagnosis of contagious agalactia.contagious agalactia is a mycoplasmal infection caused by mycoplasma agalactiae, mycoplasma mycoides subsp. mycoides lc, m. mycoides subsp. capri, mycoplasma capricolum subsp. capricolum and mycoplasma putrefaciens. identification of the causative organisms is usually performed by isolation and classical biochemical and serological tests, though this is a lengthy and cumbersome process for mycoplasmas. specific pcr assays have been developed for the identification of mycoplasma agalactiae and m. ...200717606362
in vitro antimycoplasmal activity of six jordanian medicinal plants against three mycoplasma species.the in vitro effect of six jordanian traditional medicine plant methanolic extracts were tested against 32 isolates of mycoplasma species; mycoplasma mycoides subsp. mycoides lc (6), mycoplasma capricolum subsp. capricolum (8) and m. putrefaciens (18), all isolated from either nasal swabs or milk, from sheep and goats in different regions in jordan. all mycoplasma species showed susceptibility to artemisia herba-alba and artemisia arborescens with mic ranges from 3.125-12.5 mg/ml. allium sativum ...200717969714
suppression-subtractive hybridization as a strategy to identify taxon-specific sequences within the mycoplasma mycoides cluster: design and validation of an m. capricolum subsp. capricolum-specific pcr assay.the phylogenetically related mycoplasma capricolum subsp. capricolum and m. mycoides subsp. mycoides biotype large colony are two small-ruminant pathogens involved in contagious agalactia. their respective contributions to clinical outbreaks are not well documented, because they are difficult to differentiate with the current diagnostic techniques. in order to identify dna sequences specific to one taxon or the other, a suppression-subtractive hybridization approach was developed. dna fragments ...200818234866
mycoplasma leachii sp. nov. as a new species designation for mycoplasma sp. bovine group 7 of leach, and reclassification of mycoplasma mycoides subsp. mycoides lc as a serovar of mycoplasma mycoides subsp. capri.the mycoplasma mycoides cluster consists of six pathogenic mycoplasmas causing disease in ruminants, which share many genotypic and phenotypic traits. the m. mycoides cluster comprises five recognized taxa: mycoplasma mycoides subsp. mycoides small colony (mmmsc), m. mycoides subsp. mycoides large colony (mmmlc), m. mycoides subsp. capri (mmc), mycoplasma capricolum subsp. capricolum (mcc) and m. capricolum subsp. capripneumoniae (mccp). the group of strains known as mycoplasma sp. bovine group ...200919502315
first report on the molecular prevalence of mycoplasma capricolum subspecies capripneumoniae (mccp) in goats the cause of contagious caprine pleuropneumonia (ccpp) in balochistan province of pakistan.contagious caprine pleuropneumonia (ccpp) caused by mycoplasma capricolum subspecies capripneumoniae (mccp) is a disease of goats which causes high morbidity and mortality and is reported in many countries of the world. there are probably no reports on the molecular prevalence of mccp, mycoplasma capricolum subsp. capricolum (mcc) and mycoplasma putrefaciens (mp) in balochistan and any other part of pakistan. thirty goats (n = 30) with marked respiratory symptoms were selected and procured from ...201020091126
fatal outbreak of mycoplasma capricolum pneumonia in endangered markhors.a pneumonia outbreak reduced the numbers of a wild population of endangered markhors (capra falconeri) in tajikistan in 2010. the infection was diagnosed by histologic examination and bacteriologic testing. mycoplasma capricolum subsp. capricolum was the sole infectious agent detected. cross-species transmission from domestic goats may have occurred.201122172532
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