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experimental infection of goats with an unusual strain of mycoplasma mycoides subsp. capri isolated in jordan.goats were infected experimentally with a mycoplasma (the "irbid" strain) isolated previously from a goat with contagious agalactia in northern jordan. the strain was unusual in that, although it had been identified by molecular methods as mycoplasma mycoides subsp. mycoides lc/mycoplasma mycoides subsp. capri, it showed no inhibition of growth by any of the hyperimmune rabbit antisera conventionally used to speciate members of the mycoplasma mycoides cluster. animals were infected either intrat ...200919446837
mycoplasma leachii sp. nov. as a new species designation for mycoplasma sp. bovine group 7 of leach, and reclassification of mycoplasma mycoides subsp. mycoides lc as a serovar of mycoplasma mycoides subsp. capri.the mycoplasma mycoides cluster consists of six pathogenic mycoplasmas causing disease in ruminants, which share many genotypic and phenotypic traits. the m. mycoides cluster comprises five recognized taxa: mycoplasma mycoides subsp. mycoides small colony (mmmsc), m. mycoides subsp. mycoides large colony (mmmlc), m. mycoides subsp. capri (mmc), mycoplasma capricolum subsp. capricolum (mcc) and m. capricolum subsp. capripneumoniae (mccp). the group of strains known as mycoplasma sp. bovine group ...200919502315
experimental infection of goats with an unusual strain of mycoplasma mycoides subsp. capri isolated in jordan: comparison of different diagnostic methods.ten goats were experimentally infected with a mycoplasma identified by biomolecular methods as mycoplasma mycoides subsp. capri, strain irbid which was isolated from goats in an outbreak of contagious agalactia in north jordan and defined as 'unusual', due to its serological characteristics. two groups of goats infected by the endotracheal route and by aerosol, respectively, were placed in contact with a third group of naive animals. six weeks after infection, some animals from both the infected ...201020560128
characterization of mycoplasma isolated from an ibex (capra ibex) suffering from keratoconjunctivitis in northern italy.in 2005 a mycoplasma species was isolated from ocular-conjunctival swabs from an adult male alpine ibex (capra ibex) from the valle d'aosta region, northern italy. the animal suffered from bilateral ocular discharge with diffuse inflammation, severe corneal involvement of the left eye and mild corneal opacity of the right eye. histologic examination revealed a keratoconjunctivitis characterized by lymphocytic and plasmacellular infiltration. laboratory investigations of the isolate included cult ...201020966258
controlling contagious agalactia in artificial insemination centers for goats and detection of mycoplasma mycoides subspecies capri in semen.many goat artificial insemination (ai) centers in spain have adopted new measures to control contagious agalactia (ca). to avoid the introduction of male goats carrying mycoplasma organisms subclinically in their external ear canal (auricular carriers) in these centers, two ear swabs and a blood sample are obtained from all candidate animals for polymerase chain reaction (pcr), culture (swabs) and serologic tests to detect the presence of mycoplasmas. in addition, the semen produced at these cen ...201122115814
mycoplasma mycoides, from "mycoides small colony" to "capri". a microevolutionary perspective.the mycoplasma mycoides cluster consists of five species or subspecies that are ruminant pathogens. one subspecies, mycoplasma mycoides subspecies mycoides small colony (mmmsc), is the causative agent of contagious bovine pleuropneumonia. its very close relative, mycoplasma mycoides subsp. capri (mmc), is a more ubiquitous pathogen in small ruminants causing mastitis, arthritis, keratitis, pneumonia and septicaemia and is also found as saprophyte in the ear canal. to understand the genetics unde ...201121324191
presence of contagious agalactia causing mycoplasmas in spanish goat artificial insemination centres.male goats admitted to artificial insemination centres come from herds that have shown no clinical symptoms of contagious agalactia (ca) for the last 6 mo. however, prior reports suggest that this control measure may not be completely effective. this study was designed to detect the presence of ca-causing mycoplasmas in 9 spanish centres, comprising 159 goats (147 males and 12 teaser does) of 8 different breeds. a microbiological study was conducted during 8 mo on 448 samples (318 ear swabs, 119 ...201121220162
viability of mycoplasma agalactiae and mycoplasma mycoides subsp. capri in goat milk samples stored under different conditions.control programs for contagious agalactia (ca) involve monitoring milk samples to detect this disease. this study was designed to establish the effects of the preservatives generally used in dairy laboratories and storage temperature on the viability of mycoplasma (m.) agalactiae (ma) and m. mycoides subsp. capri (mmc) in goat milk samples. in total, 1440 determinations were conducted for each mycoplasma species in milk samples subjected to different storage temperatures (refrigeration at 4°c or ...201020413227
latent infection of male goats with mycoplasma agalactiae and mycoplasma mycoides subspecies capri at an artificial insemination centre.contagious agalactia affects goats and is caused by several species of mycoplasma including mycoplasma agalactiae and mycoplasma mycoides subsp. capri (mmc). male goats, latently infected with m. agalactiae and mmc, were identified at a dairy goat breeding artificial insemination centre. in three samplings, conducted over 1 year, ear swabs were assessed for both of the above organisms using culture and pcr techniques. serological examination for antibodies against these organisms was performed a ...201019665400
comparison of culture and pcr to detect mycoplasma agalactiae and mycoplasma mycoides subsp. capri in ear swabs taken from goats.this study was designed to evaluate the validity of pcr for the direct detection of mycoplasma (m.) agalactiae and mycoplasma mycoides subsp. capri (mmc), as the two species most frequently causing contagious agalactia (ca) in goats. the pcr method was compared with the traditional culture technique to determine which method was most efficient at identifying all auricular carriers present in herds. the samples analyzed were 307 ear swabs taken from goats reared in a ca endemic area. we assessed ...201019619962
kinetics of substrate oxidation and hydrogen peroxide production by mycoplasma mycoides subsp. mycoides large colony (lc) type and mycoplasma mycoides subsp. capri.mycoplasma mycoides subsp. mycoides large colony (lc) type is a pathogen of goats causing contagious agalactia and respiratory disease, found on all continents where small ruminants are kept. it shares close genetic characteristics with m. mycoides subsp. capri. substrate oxidation by 22 strains of m. mycoides subsp. mycoides lc from nine countries was compared with that of eight strains of m. mycoides subsp. capri from five countries. there was considerable similarity in the substrates used, bu ...200919457526
mycoplasma mycoides subsp. capri and mycoplasma mycoides subsp. mycoides lc can be grouped into a single subspecies.mycoplasma mycoides subsp. capri and mycoplasma mycoides subsp. mycoides lc can be combined into one taxon on the basis of several contributions on both dna sequence and protein analyses reported in the literature. moreover, for the differentiation and identification of mycoplasmas of the "mycoides cluster", we investigated the rpob gene, encoding the beta-subunit of the rna polymerase. a segment of 527 bp of the rpob gene was amplified from 31 strains of ruminant mycoplasmas by pcr. the nucleot ...200616973118
mycoplasma mycoides subsp. capri associated with goat respiratory disease and high flock mortality.a high mortality outbreak of respiratory mycoplasmosis occurred in goats in mexico. the clinicopathologic presentation resembled contagious caprine pleuropneumonia caused by mycoplasma capricolum subspecies capripneumoniae. by using a battery of polymerase chain reaction assays, the mycoplasma associated with this outbreak was identified as mycoplasma mycoides subsp. capri.200616642877
infection of two goatherds with mycoplasma mycoides subsp. capri in hungary, evidence of a possible faecal excretion.during epidemic outbreaks in two goatherds clinical symptoms and deaths occurred in five (14%) of the 3-week-old goat kids in farm a, and in six (33%) of those in farm b. in the latter farm, three female goats aborted before the clinical symptoms in the kids emerged. mycoplasma could be isolated from both healthy and sick goat kids and from female goats, which had diseased kids or had aborted. three goat kids (one from herd a and two from herd b) were sent for post-mortem examination. in all the ...200312916690
rapid detection of contagious bovine pleuropneumonia by a mycoplasma mycoides subsp. mycoides sc capsular polysaccharide-specific antigen detection latex agglutination test.a latex agglutination test (lat) has been developed for the diagnosis of contagious bovine pleuropneumonia (cbpp). the latex microspheres were coated with mmmsc polyclonal immunoglobulin g antiserum and detected mmmsc antigen in the serum of cattle infected with cbpp and in growth medium containing mmmsc. the specific antigen recognized by this test appeared to be the capsular polysaccharide (cps). the lat recognized all 23 strains of mmmsc examined in this study, with a sensitivity level of 2 n ...200312626448
genetic and serological analysis of lipoprotein lppa in mycoplasma mycoides subsp. mycoides lc and mycoplasma mycoides subsp. capri.the genes encoding the 62-kda lipoproteins from the mycoplasma mycoides subsp. mycoides large-colony type (lc) strain y-goat and the m. mycoides subsp. capri strain pg3 were cloned and analyzed by sequencing. these two lipoproteins have been named lppa[mmymylc] and lppa[mmyca], and their corresponding genes have been named lppa[mmymylc] and lppa[mmyca], respectively. the nucleotide and deduced amino acid sequences of these two lipoproteins showed a very high degree of similarity between these tw ...199910066658
a pathological and immunohistochemical study of goat kids undergoing septicaemic disease caused by mycoplasma capricolum subsp. capricolum, mycoplasma mycoides subsp. capri and mycoplasma mycoides subsp. mycoides (large colony type).this paper studies the pathological and immunohistochemical findings in 12 kids experimentally infected with mycoplasma capricolum subsp. capricolum (mcc), m. mycoides subsp. capri (mmc) and m. mycoides subsp. mycoides (large colony type), (mmmlc). for the demonstration of mcc, mmc and mmmlc antigens an immunoperoxidase technique based on the labelled streptavidin biotin method was used in the 12 kids inoculated with mycoplasmas and the three control kids inoculated with mycoplasma medium. all 1 ...19989588108
a pathological and immunohistochemical study of caprine pleuropneumonia induced by subspecies of mycoplasma mycoides.in a population of 700 goats, 150 died; of these, 29 were necropsied. ten of the 29 goats had pleuropneumonia. mycoplasma mycoides subsp. mycoides (large colony) (mmmlc), mycoplasma mycoides subsp. capri (mmc), and pasteurella multocida were isolated from five of the pleuropneumonic goats. gross and microscopical lesions were typical of caprine pleuropneumonia (cpp), with bronchopneumonia, fibrinopurulent or fibrinonecrotic pleuropneumonia and dilatation of the interlobular septa and pleura. imm ...19968814532
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