the diversity of periodontal spirochetes by 16s rrna analysis.the purpose of this study was to examine the diversity of spirochetes in the subgingival pocket of multiple subjects with a range of periodontal conditions, including two healthy, one adult periodontitis, three acute necrotizing ulcerative gingivitis, eight refractory periodontitis, and one human immunodeficiency virus (hiv) periodontitis. the 16s rrna genes of spirochetes in plaque were amplified by polymerase chain reaction using spirochete selective primers. over 500 clones were sequenced and ...200011154403
treponema maltophilum sp. nov., a small oral spirochete isolated from human periodontal lesions.a novel culture medium for cultivation of fastidious oral anaerobes is described. this medium, omiz-pat, consists of a rich chemically defined basal medium supplemented with asialofetuin, as well as yeast extract and neopeptone fractions. addition of 1 mg of rifampin per liter and 100 mg of fosfomycin per liter allowed routine isolation of spirochetes by a limit dilution method in 96-well plates containing liquid omiz-pat. in addition to members of the four previously recognized species of oral ...19968782684
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