| ovine facioliasis (fasciola gigantica) on the ahmadu bello university farm. | outbreaks of fascioliasis (fasciola gigantica) in sheep were observed on the ahmadu bello university farm during the end of the dry season in 1972-74 and in 1978. during 1972 especially the pregnant ewes were affected. pre- and post-mortem exmaination of the affected sheep showed typical signs of acute fascioliasis; the number of flukes in the liver ranged between 77 and 239. the infections were acquired when the animals grazed near the man-made lake on the farm. a limited snail survey revealed ... | 1979 | 505587 |
| a survey on liver infections with fasciola gigantica among slaughtered animals in iraq. | | 1977 | 617942 |
| the effects of fascioliasis on the activities of some drug-metabolizing enzymes in desert sheep liver. | desert sheep experimentally or naturally infected with fasciola gigantica were used to study the influence of infection on the activities of some drug-metabolizing enzymes found in the liver. the enzymes investigated were aminopyrine n-demethylase, aniline 4-hydroxylase and udp-glucuronyltransferase. the experimental infection was confirmed histologically by detection of fasciola eggs in faeces and by measuring the activities of sorbitol dehydrogenase (sd), glutamate dehydrogenase (gd) and aspar ... | 1992 | 1617399 |
| comparative pathological study of hepatic changes induced by fasciola gigantica and gigantocotyle explanatum in javanese thin-tailed sheep. | pathological changes in the liver induced by fasciola gigantica and gigantocotyle explanatum were readily distinguishable from each other. lesions associated with the migration of immature flukes through the parenchyma were a prominent feature of infection with fasciola gigantica, whereas lesions induced by gigantocotyle explanatum were confined to the large bile ducts. the size of the hepatic lesions increased during the course of infection with fasciola gigantica and was associated with the fo ... | 1991 | 1779036 |
| first report of infectious necrotic hepatitis (black disease) among nubian goats in sudan. | in a flock of 425 female and male nubian goats in the khartoum province, an outbreak of a disease causing sudden death of 18 apparently healthy goats occurred (11 females and 7 males, 3-6 years old). adult fasciola gigantica were found in the livers of all goats and in seven of them cysticercus tenuicollis cysts. these organs showed necrotic and severe histopathological changes. clostridium novyi type b was isolated from necrotic areas of all livers and found to be highly pathogenic and toxigeni ... | 1991 | 1824134 |
| high resistance to experimental infection with fasciola gigantica in javanese thin-tailed sheep. | innate resistance of javanese thin-tailed sheep to fasciola gigantica was investigated in animals infected with single doses of 150 or 500 metacercariae and killed 4, 8, 12 or 16 weeks after infection. infected and non-infected sheep had similar values for packed cell volume, mean corpuscular volume, mean corpuscular haemoglobin concentration, serum glutamate dehydrogenase, serum gamma glutamyl transferase and serum aspartate transferase throughout the trial, except for one animal infected with ... | 1990 | 1979191 |
| [principal helminths of small ruminants in ituri (upper zaire)]. | from august 1986 to november 1987, about 180 slaughtered sheep and goats were examined at bunia (haut-zaïre) in order to establish the main helminth parasites. seventeen and fifteen species were identified in sheep and goats respectively. the occurrence of stilesia hepatica, oesophagostomum multifoliatum and muellerius capillaris as well as the absence of echinococcus granulosus constituted the original features of this check-list in the area. parasitism the most frequently encountered both in s ... | 1990 | 2369171 |
| restriction enzyme mapping of ribosomal dna can distinguish between fasciolid (liver fluke) species. | recognition sites for nine different restriction endonucleases were mapped on rdna genes of fasciolid species. southern blots of digested dna from individual worms were probed sequentially with three different probes derived from rdna of schistosoma mansoni and known to span between them the entire rdna repeat unit in that species. eighteen recognition sites were mapped for fasciola hepatica, and seventeen for fasciola gigantica and fascioloides magna. each fasciolid species had no more than two ... | 1989 | 2552311 |
| comparative study of sheep and buffalo isolates of fasciola gigantica in the intermediate host lymnaea auricularia. | a comparison was made of the biological behaviour of two isolates of fasciola gigantica, obtained from sheep and buffalo, in the aquatic snail lymnaea auricularia. the sheep isolate exhibited a lower rate of infection, slower larval development and the production of fewer cercariae. larger eggs, miracidia and metacercariae were obtained from the buffalo isolate while the rediae and cercariae were smaller. metacercariae of sheep origin were dark brown with a mortality after 80 days of storage at ... | 1989 | 2799087 |
| serum enzyme activity and bilirubin concentration in sheep experimentally infected with fasciola gigantica. | fasciola gigantica infection in sheep produced liver and lung lesions characterized by damage to blood vessels and parenchymal necrosis. in the lungs, the lesions were those of parasitic bronchopneumonia. the damage to the liver and lung tissues was accompanied by increased activity in serum of ast after 2 weeks, gd and sd after 4 to 5 weeks and ggt and 5'-nt from 8 weeks onwards. bilirubin concentration was not affected. | 1985 | 2866203 |
| studies on the prevalence and laboratory transmission of fascioliasis in animals in the kashmir valley. | in kashmir, 85.1% of cattle, 51.3% of sheep and 14.8% of goats were found infected with fasciola spp. the prevalence rate varied from 66.6 to 100.0%, 25.0 to 100% and nil to 66.0% in cattle, sheep and goats respectively in different months of the year. fasciola gigantica was the predominant species in all animal species but sheep harboured both f. gigantica and f. hepatica. the prevalence of f. hepatica infection in sheep happens to be the first report from india. lymnaea auricularia sensu stric ... | 1989 | 2920277 |
| fasciola gigantica: pathological and helminthological observations in experimental infection of yankassa lambs. | | 1988 | 3253861 |
| hepatic fascioliasis in the abattoirs of basrah. | hepatic fascioliasis due to fasciola gigantica among slaughtered animals was studied at the basrah abattoirs from october 1984 to september 1985. the infection rates were 4.8, 3.3, 0.72 and 0.13% for buffaloes, cattle, sheep and goats respectively. the approximate annual loss due to condemnation of infected livers is estimated as 29,855 iraqi dinars. | 1987 | 3446027 |
| the protective effect of irradiated metacercariae of fasciola gigantica against homologous challenge in sheep. | five lambs were each sensitized with 400 metacercariae of fasciola gigantica irradiated at 3 kr gamma rays. eight weeks later, they were each challenged together with 5 controls with 500 non-irradiated metacercariae. eight weeks later, all of the animals were necropsied and the worm burdens determined. the lambs which received the sensitizing infections had 80% less worms than the controls. the sensitized lambs also showed less hepatic damage compared with the controls as indicated by lower leve ... | 1987 | 3611708 |
| studies on naturally-occurring ovine fascioliasis in the sudan. | haematological, biochemical and pathological changes were investigated in 214 sheep naturally infected with fasciola gigantica in an endemic area in the sudan together with 82 uninfected controls. infected animals showed a clear decrease in erythrocyte counts, haemoglobin concentration and packed cell volume, a normochromic, normocytic anaemia, leucocytosis and eosinophilia. serum concentrations of the enzymes glutamate dehydrogenase, sorbitol dehydrogenase and glutamate oxaloacetic acid transam ... | 1986 | 3701020 |
| the effect of niclofolan on desert sheep experimentally infected with immature fasciola gigantica. | eight desert sheep were each infected orally with 500 metacercariae of fasciola gigantica and, after 4 weeks, four of the animals were given niclofolan orally at the recommended therapeutic dose rate of 7 mg/kg, the other four remaining as controls. one week later, the animals were slaughtered and the fasciocidal effect of the drug was evaluated on the basis of worm burden, haemogram, some plasma constituents, and gross and histopathological lesions of the liver, as indicators of efficacy. the t ... | 1985 | 4094030 |
| [therapy and early diagnosis of fasciola gigantica infection in sheep]. | | 1969 | 5388487 |
| [the effect of hetol on immature and mature stages of fasciola gigantica in artificially infected sheep]. | | 1967 | 5628510 |
| [effectiveness of zanil against immature and sexually mature stages of fasciola gigantica in artifically infected sheep]. | | 1968 | 5692951 |
| [additional data on superficial argentophilic structures of the miracidium and cercaria of fasciola gigantica (cobbold, 1855) (trematoda:fasciolidae) and on the epidemiology of this parasite]. | the life cycle of a senegalese strain of fasciola gigantica was carried out in france from adults recovered in the liver of naturally infected cattle (bos taurus). laboratory-reared lymnaea natalensis experimentally infected shed numerous cercariae. metacercariae were used for successful infection of sheep. in the miracidium, variations are observed in the disposition of the medio-ventral epithelial cell of the second tier. the distribution of the superficial argentophilic structures is similar ... | 1980 | 6164333 |
| infectivity and pathogenicity of fasciola gigantica in west african dwarf sheep and goats. | the infectivity and pathogenicity of fasciola gigantica for sheep and goats were investigated in animals infected with single doses of between 200 and 2,000 metacercariae. acute fascioliasis occurred in sheep infected with 1,000 to 2,000 cysts in which 10 to 17% of the cysts became established. the infected animals died 80 to 90 days after infection. in goats given the same infective doses death from acute fascioliasis occurred earlier (70 to 83 days after infection) and 12 to 38% of cysts becam ... | 1984 | 6485107 |
| efficacy of ivermectin against internal parasites of sheep. | ivermectin in an oral formulation was evaluated by the non-parametric method against a wide range of endoparasites of sheep in 15 trials involving a total of 297 sheep. ivermectin at 200 micrograms/kg was more than 80% effective in more than 80% of the treated animals (i.e. class a) against induced infestations of 3rd and 4th stage larvae and adults of chabertia ovina, dictyocaulus filaria, haemonchus contortus (including a benzimidazole-resistant strain), nematodirus spathiger, oesophagostomum ... | 1984 | 6549472 |
| specific inhibitors and substrates studies on the cholinesterases of fasciola gigantica from sheep and goats. | | 1983 | 6871931 |
| red blood cell survival and faecal clearance in sheep infected with fasciola gigantica. | | 1981 | 7320242 |
| time-course analysis of antibody response by eitb and elisa before and after chemotherapy in sheep infected with fasciola gigantica. | the time-course analysis of the antibody response to fascicola gigantica infection in sheep was studied by enzyme-linked immunosorbent assay (elisa) and enzyme-linked immunoelectrotransfer blot (eitb). sera from sheep experimentally infected with f. gigantica were reacted with excretory-secretory antigens of the worm before and after chemotherapy with oxyclozanide. in elisa, there was a significant increase in anti-fasciola antibody by 2 weeks after infection and there was a sharp decrease in an ... | 1995 | 7571329 |
| immunodiagnosis of fasciolosis in ruminants using a 28-kda cysteine protease of fasciola gigantica adult worms. | a homogeneous extract of a 28-kda cysteine protease of fasciola gigantica adult worms was used as the antigen for immunodiagnosis of fasciolosis in cattle, sheep and goats using the falcon assay screening test enzyme-linked immunosorbent assay technique. this antiprotease assay technique was found to be very rapid and sensitive and may be useful as a supplementary method for the diagnosis of fasciolosis in ruminants. | 1995 | 7660568 |
| [immunodiagnosis of fasciola gigantica fascioliasis by elisa technique in senegal. preliminary observations in 2 lambs]. | the purpose of this work was to investigate the use of the elisa test for the diagnosis of fasciolosis (fasciola gigantica) in sheep, in senegal, using fasciola gigantica metabolic excretion-secretion products as antigens. coprological analysis results were negative until the fourteenth week following infection; using the elisa test, the first anti-fasciola gigantica antibodies can be detected as early as the fourth week following infection. | 1994 | 7709030 |
| [epidemiology of gastrointestinal helminthiasis in small ruminants in the tree-cropping pasture zone in senegal]. | an epidemiological survey on gastrointestinal helminthiasis in 51 sheep and 51 goats was held in the tree-cropping pasture region in senegal from october 1990 to september 1991. all the animals examined were infected with at least 1 helminth species. three trematodes (fasciola gigantica, schistosoma bovis, amphistomatids), 2 cestodes (moniezia expansa, cysticercus tenuicollis) and 9 nematodes were identified. the most important parasite in sheep was haemonchus contortus while trichostrongylus co ... | 1995 | 7735304 |
| liver fluke infections in livestock in borno state, nigeria. | a survey was conducted on the prevalence of fasciola gigantica and dicrocoelium hospes eggs in gall bladders of cattle and small ruminants (sheep and goats) slaughtered between november 1987 and december 1988 in maiduguri, borno state, nigeria. prevalence rates of 42.7% and 5.7% were recorded for f. gigantica and d. hospes eggs, respectively, in 246 cattle and 718 sheep and goats examined. out of the 246 cattle examined, 52 (21.1%), 45 (18.3%) and 8 (3.3%) excreted eggs of f. gigantica, d. hospe ... | 1993 | 8122358 |
| effects of ration and fasciola infection on some blood parameters of ewes. | this study was conducted using fourty cross-bred ewes in 5 groups of which one was kept as a control and the others were infected with metacercariae of fasciola gigantica twice at the beginning of the experiment and 200 days from the first. the four infected groups fed on basal ration or high protein ration with or without mineral mixture. blood samples were taken from the five groups to assess the concentrations of plasma fibrinogen and serum total proteins, albumin, globulin, calcium and inorg ... | 1993 | 8308350 |
| prevalence of hepatic helminths and associated pathology in impala (aepyceros melampus) in swaziland. | the livers of impala (aepyceros melampus) collected in the mlawula-mbuluzi-simunye nature reserve and protected area complex in northeastern swaziland from october 1985 to september 1986 were infected with three species of helminths: a nematode (cooperioides hepaticae), a cestode (stilesia hepatica), and a trematode (fasciola gigantica). cooperioides hepaticae caused a severe cholangitis. lambs were infected at 1.5 to 2 mo of age, and the prevalence increased to 100% by 5 mo of age. the prevalen ... | 1996 | 8627927 |
| acquired resistance of merino sheep against fasciola gigantica. | merino sheep acquired resistance against fasciola gigantica, which is contrary to previous observations of infections with f. hepatica in that breed. the acquired resistance was manifest against young adult parasites. st. croix sheep had previously been shown to have more resistance than european sheep against f. hepatica after primary infection; however, in f. gigantica infections in the present study there was no difference between the resistance levels of the breeds. antigenic analysis of the ... | 1996 | 8897512 |
| detection of circulating fasciola gigantica antigen in experimental and natural infections of sheep with fasciolosis. | detection of circulating fasciola gigantica antigen was performed in sera of sheep with experimental and natural f. gigantica infections using the direct enzyme-linked immunosorbent assay method. sera from sheep with monoinfections of schistosoma bovis, dicrocoelium hospes and paramphistomum microbothrium were included in the assay to ascertain specificity. circulating f. gigantica antigen (cfa) was detected as early as 1 week after infection in the experimentally infected sheep. no detectable c ... | 1996 | 8916398 |
| approaches to the control of fasciolosis in ruminants. | approaches to the control of fasciolosis in ruminants are compared for developed countries, and for developing countries with particular reference to regions growing irrigated rice. in all environments problem definition and investigation are based on one or more of observation, abattoir surveys, faecal egg counts, tracer animals and snail studies. in developed countries each husbandman grazes a large number of animals, controls access to pasture and water, and markets high value products. strat ... | 1996 | 8923144 |
| resistance of indonesian thin tail sheep against fasciola gigantica and f. hepatica. | high resistance of indonesian thin tail (itt) sheep against fasciola gigantica has been confirmed. naive itt sheep had only 17% of the number of mature parasites collected from control st. croix sheep. in contrast, the level of resistance of itt sheep against f. hepatica was the same as that of the low resistance merino breed after both primary and secondary infections. it is suggested that resistance of itt sheep against f. gigantica was manifested in two phases. the major phase appeared to be ... | 1997 | 9066053 |
| the isolation of fasciola gigantica-specific antigens and their use in the serodiagnosis of fasciolosis in sheep by the detection of circulating antigens. | the 17kda and 69kda polypeptide antigens which are specific to fasciola gigantica were excised from polyacrylamide gels and used for the immunization of rabbits to raise monospecific antisera against these polypeptides. these sera were labelled with horseradish peroxidase and used for the detection of circulating 17 kda and 69 kda antigens by a direct enzyme-linked immunosorbent assay in the sera of sheep that were experimentally or naturally infected with f. gigantica. the 17 kda antigen was de ... | 1997 | 9066072 |
| evidence for a major gene determining the resistance of indonesian thin tail sheep against fasciola gigantica. | the genetic basis of the high resistance of indonesian thin tail (itt) sheep against fasciola gigantica has been studied by measuring the resistance levels of itt sheep, st. croix sheep and their f2 and f3 crossbreds. all of the sheep were bred and reared in the same environment. approximately half of the f2 and f3 crossbreds were as resistant as the itt parental breed, one-quarter had the low resistance of the st. croix parental breed and the rest had an intermediate level of resistance. it is ... | 1997 | 9106952 |
| the effect of experimental fascioliasis on the pharmacokinetics of antipyrine and sulphadimidine in desert sheep. | healthy adult male desert sheep were experimentally infected with fasciola gigantica, to investigate the influence of experimental fasciolasis on the pharmacokinetics of antipyrine and sulphadimidine. each animal received 500 metacercariae orally. the experimental infection was confirmed histologically, by detection of fasciola eggs in faeces and by measuring the activities of the enzymes sorbitol dehydrogenase (sd), glutamate dehydrogenase (gd) and aspartate aminotransferase (ast) in plasma dur ... | 1997 | 9185081 |
| acquisition of resistance against fasciola gigantica by indonesian thin tail sheep. | the plasma levels of gldh and the dynamics of development of the numbers of surface lesions on livers, and numbers of parasites, within two to three weeks of a challenge infection, indicated that the major component of the high resistance of indonesian thin tail (itt) sheep against fasciola gigantica was acquired, and acted against juvenile parasites. few parasites reached the livers of itt (exposed) sheep, but many had been inhibited, probably in the wall of the jejunum. itt (naive) sheep showe ... | 1997 | 9477508 |
| an erratic parasitism found in the lungs of sheep during experimental infection with fasciola gigantica (japanese strain). | an erratic parasitism was observed in the lungs of sheep experimentally infected with fasciola gigantica at autopsy 14 weeks after inoculation. macroscopically, several hyperaemia of 7.5-12.5 mm in diameter were found on the surface of the lungs. juvenile flukes detected in the lungs were much smaller than those in the liver of the same sheep. a slight inflammatory reaction was observed in the lungs and it is likely that the flukes had migrated in the pleural cavity for some time. occasionally, ... | 1998 | 9765374 |
| a comparison of serum biochemical changes in two breeds of sheep (red masai and dorper) experimentally infected with fasciola gigantica. | twelve red masai and 12 dorper sheep aged between 6 and 9 months, were acquired from a fluke-free area and sheep of each breed divided into two equal groups of six. each animal in one group of each breed was experimentally infected with 400 viable metacercariae of fasciola gigantica. the other groups acted as uninfected controls. blood samples were taken at weekly intervals for the determination of serum bilirubin, albumin, and gamma glutamyl transferase levels. following the establishment of in ... | 1999 | 10396762 |
| studies on the viability of metacercariae of fasciola gigantica. | the viability of metacercariae of fasciola gigantica was tested by in vitro and in vivo methods. in vitro testing was based upon the motility of juvenile flukes within the inner cyst as examined under the light microscope. in vivo testing was undertaken through experimental infections of rabbits (two groups) and natural definitive hosts, lambs (one group). in the first group, out of six rabbits each given 25 metacercariae, worm establishment only took place in one rabbit with a single fluke reco ... | 1999 | 10431377 |
| effect of fasciola gigantica somatic extracts and excretory/secretory products on superoxide production by activated neutrophils. | somatic extracts and excretory/secretory (es) products from fasciola gigantica suppress the release of toxic oxygen intermediates by activated sheep neutrophils. at the concentrations used both the somatic extracts and es products had no effect on neutrophil viability and in both cases the inhibitory effect was reversible. in the somatic extracts, heating or treatment with trypsin destroyed the inhibitory effect. however, the es products contained at least two immunomodulatory factors. one was l ... | 1999 | 10435794 |
| immune responses in indonesian thin tail and merino sheep during a primary infection with fasciola gigantica: lack of a specific igg2 antibody response is associated with increased resistance to infection in indonesian sheep. | after a primary infection with fasciola gigantica, the immune responses in a resistant (indonesian thin tail) and a susceptible (merino) breed of sheep were analysed. the number of adult flukes recovered from the livers of the indonesian thin tail sheep were significantly lower than those found in the merino animals. on days 8, 14 and 25 p.i., indonesian thin tail sheep exhibited a significantly higher eosinophilia than merino sheep, whereas neutrophilia was significantly elevated in the indones ... | 1999 | 10501613 |
| comparative parasitological and haematological changes in two breeds of sheep infected with fasciola gigantica. | twelve each of red masai and dorper sheep, aged between 6 and 9 months, were acquired from a fasciola-free area of eastern kenya. each breed was divided into two groups of 6. the sheep in one group of each breed were experimentally infected with 400 viable metacercariae of fasciola gigantica. the other group of 6 sheep of each breed remained as uninfected controls. the animals were monitored regularly for any evidence of disease. blood samples taken weekly revealed a general reduction in red cel ... | 1999 | 10599131 |
| camel: a new egyptian host for fasciola gigantica. | f. gigantica is a known liver fluke in egypt. infection has been reported in sheep, goats, buffaloes, cows and donkeys. human cases have been reported from several governorates. this paper records camels as an additional host for f. gigantica in egypt. in such materials worms attained a length of 6.0-6.3 cm. | 2000 | 10946507 |
| early immunodiagnosis of fasciolosis in ruminants using recombinant fasciola hepatica cathepsin l-like protease. | a diagnostic elisa with recombinant fasciola hepatica cathepsin l-like protease as antigen was developed to detect antibodies against f. hepatica in sheep and cattle. the recombinant cathepsin l-like protease was generated by functional expression of the cdna from adult stage f. hepatica flukes in saccharomyces cerevisiae. specificity and sensitivity of the cathepsin l enzyme-linked immunosorbent assay (elisa) was assessed using sera from sheep and calves experimentally or naturally mono-infecte ... | 2001 | 11336755 |
| evaluation of cercarial antigen for the serodiagnosis of fasciolosis in experimentally and naturally infected sheep. | the value of cercarial antigen for diagnosis of experimental and natural sheep fasciolosis was studied by enzyme linked immunosorbent assay (elisa) and enzyme linked immunotransfer blot (eitb). in elisa, the antibody levels of experimentally infected sheep with fasciola gigantica appeared at 2 weeks post infection (pi), gradually increased till 7 weeks pi and nearly remained at the same level from 7 to 13 weeks pi (the end of experiment). also, the sensitivity and specificity of cercarial antige ... | 2001 | 11337126 |
| immunization of sheep against fasciola gigantica with glutathione s-transferase. | glutathione s-transferase in fasciola gigantica (fggst) was isolated by affinity chromatography, by which highly purified enzyme was obtained. fggst on the sds-page showed three protein bands ranging 24.5-26.5kda. glutathione s-transferase (gst) activity was determined by habig method. fggst was evaluated as vaccine alone or in combination with either aluminum hydroxide or saponin in sheep against f. gigantica infection. elisa was used for detection of anti-fggst igg. after vaccination, all shee ... | 2002 | 11900929 |
| 28 kda fasciola gigantica cysteine proteinase in the diagnosis of prepatent ovine fasciolosis. | coprological confirmation of ovine fasciolosis in the field, prior to out breaks of the disease and/or strategic antifluke medication, seem to be of little consequence. efforts are, therefore, being made to evolve a putative antigen specific to serodiagnostic test for early diagnosis during prepatency. in the present investigation, 28 kda cysteine proteinase was used in eli sa and western blot to detect fasciola gigantica antibodies and further dipstick-elisa was developed for field application, ... | 2002 | 12423935 |
| a pcr-rflp assay for the distinction between fasciola hepatica and fasciola gigantica. | fascioliasis is of well-known veterinary importance and an increasing human health problem, with reported cases in the five continents. the causative agents, fasciola hepatica and fasciola gigantica, present geographical distributions, which overlap in many regions of africa and asia, and in which the differentiation of both species is usually difficult because of the many variations in their morphological characteristics. moreover, in humans, liver fluke classification cannot be achieved by cli ... | 2002 | 12477436 |
| fasciola gigantica and fascioliasis in turkey. | | 1964 | 14103225 |
| characterisation of fasciola species from mainland china by its-2 ribosomal dna sequence. | isolates of fasciola (platyhelminthes: trematoda: digenea) from different host species and geographical locations in mainland china were characterised genetically. the second internal transcribed spacer (its-2) of nuclear ribosomal dna (rdna) was amplified from individual trematodes by polymerase chain reaction (pcr), and the representative amplicons were cloned and sequenced. the length of the its-2 sequences was 361-362bp for all chinese fasciola specimens sequenced. while there was no variati ... | 2004 | 15019145 |
| comparison of humoral response in sheep to fasciola hepatica and fasciola gigantica experimental infection. | humoral response of sheep to f. gigantica was compared with the well known humoral response to f. hepatica, in order to explain the difference of susceptibility of sheep to these two parasites. in this work, a lesser susceptibility of sheep to f. gigantica than to f. hepatica infection was confirmed. humoral response to f. hepatica infection is similar to that previously described by several authors. igg level of f. gigantica infected sheep increased from week 2 post-infection (2wpi) and display ... | 2004 | 15224576 |
| detection of genetic variabiltiy in nonhuman isolates of fasciola hepatica and fasciola gigantica by the rapd-pcr technique. | the present study shows the molecular characterization of fasciola gigantica and f. hepatica isolates collected from cows and sheep, using the random amplified polymorphic dna fragments-polymerase chain reaction (rapds-pcr) technique. optimal standardization of amplification conditions and thermocyclation were made, using genetic markers. the methodology used compared the genetic pattern between the two species (inter-species) and inside each species (intra-species) between cow and sheep and the ... | 2004 | 15287188 |
| humoral immune response against fasciola gigantica fatty acid binding protein. | recombinant fatty acid binding protein of fasciola gigantica was expressed in escherichia coli and purified by nickel chelating affinity chromatography. the recombinant protein along with native fatty acid binding protein (fabp) isolated from the parasite were evaluated for their potential in the diagnosis of f. gigantica infection in sheep, cattle and buffaloes, both by elisa and western blotting. results of this study indicate that there is no humoral immune response generated against this pro ... | 2004 | 15350662 |
| comparison of modulation of sheep, mouse and buffalo lymphocyte responses by fasciola hepatica and fasciola gigantica excretory-secretory products. | in order to explain the difference in susceptibility to fasciola hepatica and f. gigantica between animal species, the activity of their excretory-secretory products (fhesp and fgesp, respectively) on concanavalin a (cona)-induced proliferation of different animal species (sheep, mouse and buffalo) lymphocytes was compared. at high doses, fhesp inhibited proliferation of lymphocytes of all the animal species tested, and at low doses they inhibited the proliferation of sheep lymphocytes and incre ... | 2005 | 15711847 |
| evaluation of a commercially available enzyme-linked immunosorbent assay for detecting antibodies to fasciola hepatica and fasciola gigantica in cattle, sheep and buffaloes in australia. | a commercially available elisa for detecting antibodies to liver fluke was evaluated for use in australia. milk and serum samples from cattle and sheep in which infection with fasciola hepatica was confirmed by detection of eggs in faeces were used to estimate sensitivity. similar samples collected from cattle and sheep outside the f. hepatica-endemic area were used to estimate specificity. the elisa was also evaluated for detecting antibodies to f. hepatica in milk from sheep and antibodies to ... | 2005 | 15925723 |
| characterization of specific and cross-reacting antigens of fasciola gigantica by immunoblotting. | somatic antigens of f. gigantica, g. explanatum, s. spindale and hydatid cyst ingredients were analysed to identify the cross-reactive antigens among them using western blot technique. when probed with f. gigantica infected cattle sera, the immunodominant 156 kda and 28 kda proteins of f. gigantica was found common amongst the antigens prepared from hydatid cysts ingredients like germinal layer, fertile and sterile, hydatid fluid, fertile and sterile, while another protein of 34 kda was shared b ... | 2005 | 15952043 |
| urea cycle of fasciola gigantica: purification and characterization of arginase. | the ornithine-urea cycle has been investigated in fasciola gigantica. agrinase had very high activity compared to the other enzymes. carbamoyl phosphate synthetase and ornithine carbamoyltransferase had very low activity. a moderate enzymatic activity was recorded for argininosuccinate synthetase and argininosuccinate lyase. the low levels of f. gigantica urea cycle enzymes except to the arginase suggest the urea cycle is operative but its role is of a minor important. the high level of arginase ... | 2005 | 16125991 |
| fasciola hepatica and fasciola gigantica: comparison of cellular response to experimental infection in sheep. | cellular responses to fasciola gigantica and to fasciola hepatica infection in sheep were compared. eosinophil numbers increased more quickly and strongly in f. gigantica-infected sheep than in f. hepatica-infected sheep. in both groups, peripheral blood mononuclear cell (pbmc) proliferation in response to the parasitic excretory-secretory products (esp) showed similar kinetics. interferon-gamma (ifn-gamma) production by esp-stimulated pbmc was early and showed similar kinetics in both groups. i ... | 2005 | 16126202 |
| fasciola gigantica cathepsin-l cysteine proteinase in the detection of early experimental fasciolosis in ruminants. | cathepsin-l cysteine proteinase was purified from fasciola gigantica regurgitant by two-step alcoholic fractionation, followed by ion-exchange chromatography. the purification strategy was evolved to eliminate other contaminating proteins co-precipitating with the purified proteinase during alcoholic fractionation. the enzyme was stable on long-term storage at -20 degrees c rendering it more suitable for field diagnostic use. the purified cathepsin-l cysteine proteinase was assayed for detection ... | 2005 | 16222528 |
| detection of coproantigens by sandwich elisa in sheep experimentally infected with fasciola gigantica. | two monoclonal antibodies to fasciola gigantica excretory/secretory (es) antigens were used in a sandwich elisa for the detection of fasciola antigens in faeces of 9 sheep experimentally infected with 300 metacercariae of f. gigantica. the detection of coproantigens was found in four of the seven sheep within 5 weeks of infection, and within 7 weeks of infection coproantigens were detected in all seven of the sheep. this technique was compared to an indirect elisa for the detection of anti-fasci ... | 2004 | 16493398 |
| serum antibody isotype responses of fasciola-infected sheep and cattle to excretory and secretory products of fasciola species. | this study investigated the immunoglobulin isotype responses of sheep and cattle chronically infected with fasciola hepatica and fasciola gigantica to adult f. hepatica excretory/secretory products (fh-es) or f. gigantica excretory/secretory products (fg-es), respectively. an antibody enzyme-linked immunosorbent assay (ab-elisa) was used to determine serum antibody (total ig, igg(1), igm, igg(2) and iga) responses. at necropsy, the mean number of flukes recovered was lower in cattle than in shee ... | 2006 | 16797844 |
| the serum glucose and beta-hydroxybutyrate levels in sheep with experimental fasciola hepatica and fasciola gigantica infection. | the influence of fasciola hepatica and fasciola gigantica infection on serum glucose and beta-hydroxybutyrate (beta-hob) in sheep was evaluated. this was done by setting up two groups of sheep. the first group (n=13) was split in two sub-groups, one experimentally infected with f. hepatica (n=9) and the other (n=4) as uninfected control. a second group consisting of a sub-group experimentally infected with f. gigantica (n=9) the other sub-group (n=6) left as uninfected control was also set up. t ... | 2007 | 17011709 |
| host responses during experimental infection with fasciola gigantica or fasciola hepatica in merino sheep i. comparative immunological and plasma biochemical changes during early infection. | this study reports the early biochemical changes in plasma, comparative host-immune responses and parasite recovery data in merino sheep during the first 10 weeks of infection with fasciola gigantica and fasciola hepatica. one group of sheep were uninfected, four groups of sheep received incremental challenge doses of f. gigantica metacercariae (50, 125, 225 and 400, respectively) and the sixth group was challenged with 250 f. hepatica metacercariae. at 10 weeks post infection (wpi), sheep chall ... | 2007 | 17069976 |
| the effect of temperature and humidity on longevity of metacercariae of fasciola gigantica. | studies were undertaken on the viability of the metacercariae of fasciola gigantica when stored in water at 13 degrees c for periods up to 23 weeks, exposed to the sunlight for up to 8 h or stored at a range of temperatures and humidities for up to 10 weeks. excysted metacercariae were catergorized microscopically as viable (motile and undamaged), dubious (not motile and undamaged) or dead (visible necrosis). the infectivity of viable and dubious metacercariae and unselected reference metacercar ... | 2006 | 17165607 |
| peritoneal lavage cells of indonesian thin-tail sheep mediate antibody-dependent superoxide radical cytotoxicity in vitro against newly excysted juvenile fasciola gigantica but not juvenile fasciola hepatica. | indonesian thin-tail (itt) sheep resist infection by fasciola gigantica by an immunological mechanism within 2 to 4 weeks of infection yet are susceptible to f. hepatica infection. studies of itt sheep show that little liver damage occurs following f. gigantica infection, suggesting that the invading parasites are killed within the peritoneum or shortly after reaching the liver. we investigated whether cells isolated from the peritoneums of itt sheep could kill newly excysted juvenile f. giganti ... | 2007 | 17210661 |
| the prevalence and economic significance of fasciola gigantica and stilesia hepatica in slaughtered animals in the semi-arid coastal kenya. | fasciola gigantica and stilesia hepatica constrain ruminant productivity. the prevalence and economic losses caused by f. gigantica and s. hepatica in the ruminant production systems of taveta division of kenya were estimated in a retrospective appraisal of the slaughter records on the total number of animals slaughtered and livers condemned over the period 1989 to 2004. only liver condemnations based on infestation by f. gigantica and s. hepatica were considered for purposes of this study. live ... | 2006 | 17243475 |
| fasciola gigantica cathepsin l proteinase-based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis. | sheep fasciolosis is a devastating burden for the livestock industry. we herein report on immunodiagnosis of fasciolosis, and significant protection of sheep against challenge infection with fasciola gigantica following immunization with a peptide based on the h-asp(110)-lys-ile-asp-trp-arg-glu-ser-gly-tyr-val-thr-glu-val(123)-oh (fas14p) sequence of f. gigantica cathepsin l-cysteine proteinase. this sequence was synthesized in three different forms: as n(alpha) acetylated (ac-asp(110)-lys-ile-a ... | 2008 | 17549696 |
| fasciola gigantica: enzymes of the ornithine-proline-glutamate pathway--characterization of delta1-pyrroline-5-carboxylate dehydrogenase. | ornithine aminotransferase (oat), proline oxidase (po), delta 1-pyrroline-5-carboxylate reductase (p5cr), and delta 1-pyrroline-5-carboxylate dehydrogenase (p5cd) were assessed in fasciola gigantica. all enzymes are involved in the conversion of ornithine into glutamate and proline. high levels of p5cd suggest that the direction of the metabolic flow from ornithine is more toward glutamate than proline. f. gigantica p5cd1 and p5cd2 were separated from the majority of contaminating proteins in cr ... | 2008 | 17655846 |
| distribution of metacercariae of fasciola gigantica on rice straw. | | 2006 | 17682596 |
| fasciola gigantica excretory-secretory products for immunodiagnosis and prevention of sheep fasciolosis. | excretory-secretory products (esp) products of ex vivo fasciola gigantica adult worms were used for immunodiagnosis of sheep experimental infection with f. gigantica and natural infection with fasciola spp. by enzyme-linked immunosorbent assay (elisa) and western blotting. specific igg antibody binding to native or denatured esp was detected as early as 2 weeks after experimental sheep infection with 100 or 200 metacercariae. no specific igg antibody binding was displayed by sera obtained from 1 ... | 2007 | 17897786 |
| genetic characterisation of fasciola samples from different host species and geographical localities revealed the existence of f. hepatica and f. gigantica in niger. | in the present study, 16 samples representing fasciola (platyhelminthes: trematoda: digenea) from sheep and cattle from seven geographical locations in niger were characterized genetically by sequences of the first (its-1) and second (its-2) internal transcribed spacers (its) of nuclear ribosomal dna (rdna). the its rdna was amplified from individual liver flukes by polymerase chain reaction (pcr), and the amplicons were sequenced directly. the lengths of the its-1 and its-2 sequences were 422 a ... | 2008 | 18183422 |
| fasciola hepatica and fasciola gigantica: cloning and characterisation of 70 kda heat-shock proteins reveals variation in hsp70 gene expression between parasite species recovered from sheep. | fasciola hepatica and fasciola gigantica are trematode parasites responsible for fasciolosis, a disease of ruminant animals which is also increasingly recognised as a disease in humans. by biochemical and in silico methods, we have cloned and characterised the 70 kda heat-shock proteins (hsp70s) of f. hepatica and f. gigantica. the nucleotide and protein sequences for hsp70 were found to be 98% and 99% identical between liver fluke species, respectively, and to encode conserved amino acid motifs ... | 2008 | 18190913 |
| fasciola gigantica: purification and characterization of adenosine deaminase. | nucleotidase cascades (apyrase, 5' nucleotidase, and adenosine deaminase (ada) were investigated in the parasitic trematode fasciola gigantica. ada had the highest activity in the nucleotidase cascades. adenosine deaminase was purified from f. gigantica through acetone precipitation and chromatography on cm-cellulose. two forms of enzyme (adai, adaii) were separated. adaii was purified to homogeneity after chromatography on sephacryl s-200. the molecular mass was 29 kda for the native and denatu ... | 2008 | 18436213 |
| host responses during experimental infection with fasciola gigantica and fasciola hepatica in merino sheep ii. development of a predictive index for fasciola gigantica worm burden. | this study reports on the predictive relationship between serological, immunological and pathological responses following experimental inoculation with incremental doses of fasciola gigantica in sheep. fifty, 6-month-old, naive merino wethers were allocated to one of 5 experimental groups, four of which received 50, 125, 225 and 400 metacercariae, respectively, whilst a 5th group acted as non-inoculated control. strong individual correlations were observed between liver score, gldh (glutamate de ... | 2008 | 18468797 |
| using indirect elisa to assess different antigens for the serodiagnosis of fasciola gigantica infection in cattle, sheep and donkeys. | an indirect enzyme-linked immunosorbent assay (ielisa) was evaluated for its diagnostic capability in detecting antibodies against fasciola gigantica infection in cattle, sheep and donkeys sera using crude worm, excretory-secretory and glutathione s-transferase antigens prepared from adult liver fluke. presence of f. gigantica worms at post-mortem examination of cattle, sheep and donkey's livers was taken as a gold standard for the evaluation of the assay. the diagnostic sensitivity, specificity ... | 2009 | 18947842 |
| identification and differentiation of fasciola hepatica and fasciola gigantica using a simple pcr-restriction enzyme method. | accurate morphological differentiation between the liver fluke species fasciola hepatica and fasciola gigantica is difficult. we evaluated pcr-restriction enzyme profiles of internal transcribed spacer 1 (its1) that could aid in their identification. fifty f. hepatica and 30 f. gigantica specimens were collected from different hosts in three provinces of iran. for dna extraction, we crushed fragments of the worms between two glass slides as a new method to break down the cells. dna from the crus ... | 2010 | 19769969 |
| fasciola gigantica: larval productivity of sheep-derived miracidia in radix natalensis and galba truncatula. | experimental infections of egyptian radix natalensis and french galba truncatula with miracidia of fasciola gigantica of sheep were carried out to determine the larval productivity of this parasite. rediae and cercariae were thus counted in snails dissected at regular intervals from day 21 to day 49 postexposure (p.e.) at 24 degrees c, while cercarial shedding was studied in other two groups of snails after day 30 p.e. at day 49, the total number of free rediae and that of cercariae-containing r ... | 2009 | 20120750 |
| evaluation of the diagnostic potential of different immunological techniques using polyclonal antibodies against fasciola gigantica excretory/secretory antigens in sheep. | the early detection of fasciola antigens (ags) in serum or stool could be more valuable in diagnosis as early treatment would be applied before irreparable damage occurs. in this study, fresh adult fasciola gigantica (f. gigantica) worms were cultivated for 16 hrs. excretory/secretory (e/s) ags were extracted from the culturemedium and used to raise rabbit antibodies (abs) to fasciola. the purified abs were then used in sandwich elisa (s-elisa) to detect fasciola ags in serum and stool samples f ... | 2008 | 20306670 |
| comparative analysis of two fatty acid binding proteins from fasciola gigantica. | fatty acid binding proteins are considered to be promising vaccine candidates against trematodiasis. in order to provide additional information about their function in fasciola gigantica we performed a comparative analysis of fgfabp1 and fgfabp3, two isoforms with quite different isoelectric points of 4.9 and 9.9 and 67% sequence identity. both are expressed in the juvenile and adult parasite but differ in their tissue-specific distribution. in addition, the sequence of fabp3 is identical in f. ... | 2010 | 20550752 |
| innate and adaptive resistance of indonesian thin tail sheep to liver fluke: a comparative analysis of fasciola gigantica and fasciola hepatica infection. | in the current study, three independent trials directly compared fasciola gigantica and fasciola hepatica infection of itt sheep. in all trials, f. hepatica infection resulted in higher worm burden recoveries and greater physiological damage to itt sheep. developmental differences of the two fasciola species were also observed during the first twelve weeks of a primary infection, where the migration and growth of f. hepatica was more rapid than f. gigantica. various immunological blood parameter ... | 2011 | 21320754 |
| hybridization experiments indicate incomplete reproductive isolating mechanism between fasciola hepatica and fasciola gigantica. | summaryexperiments on hybridization between fasciola hepatica and fasciola gigantica were carried out to clarify whether a reproductive isolating mechanism appears between the two fasciola species. molecular evidence for hybridization was based on the dna sequence of the internal transcribed spacer 1 (its1) region in nuclear ribosomal dna, which differs between the species. the results suggested that there were not pre-mating but post-mating isolating mechanisms between the two species. however, ... | 2011 | 21767436 |
| molecular characterization of fasciola gigantica from mauritania based on mitochondrial and nuclear ribosomal dna sequences. | fasciolosis caused by fasciola hepatica and fasciola gigantica (platyhelminthes: trematoda: digenea) is considered the most important helminth infection of ruminants in tropical countries, causing considerable socioeconomic problems. from africa, f. gigantica has been previously characterized from burkina faso, senegal, kenya, zambia and mali, while f. hepatica has been reported from morocco and tunisia, and both species have been observed from ethiopia and egypt on the basis of morphometric dif ... | 2011 | 21763690 |
| resistance to liver fluke infection in the natural sheep host is correlated with a type-1 cytokine response. | indonesian thin-tail (itt) sheep can resist infection with fasciola gigantica but not f.-áhepatica and presents an ideal model to investigate the mechanisms of liver fluke resistance in a natural host. this study examines the local and systemic immune responses of sheep during fasciola infection and demonstrates that different anatomical tissues display distinct cytokine profiles consistent with liver fluke migration. the study also reveals a significant difference in the cytokine and antibody p ... | 2011 | 21711360 |
| Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1). | The present study compared the genetic variation among 19 different isolates of Fasciola hepatica from cattle and sheep in different areas of Iran using sequence data for mitochondrial DNA gene, the subunit 1 of cytochrome C oxidase gene (CO1). Four different CO1 genotypes were detected among F. hepatica isolates that showed five variable nucleotide positions (accession nos.; GQ398051, GQ398052, GQ398053, GQ398054). Nucleotide sequence variation among 19 isolates for CO1 analyzed in this study r ... | 2011 | 22186976 |
| cholangiocarcinoma in magnetic resonance cholangiopancreatography and fascioliasis in endoscopic ultrasonography. | fascioliasis is a worldwide zoonotic infection with fasciola hepatica and fasciola gigantica. the zoonoses are particularly endemic in sheep-raising countries and are also endemic in iran. typical symptoms that may be associated with fascioliasis can be divided by phases of the disease, including the acute or liver phase, the chronic or biliary phase, and ectopic or pharyngeal fascioliasis. cholestatic symptoms may be absent, and in some cases diagnosis and treatment may be preceded by a long pe ... | 2011 | 22110417 |
| update on trematode infections in sheep. | trematode parasites live in the liver, fore stomachs or blood vessels of a wide range of animals and humans. most of them have a special economic and veterinary significance. liver fluke disease of sheep and other animal species is caused by the common liver fluke fasciola hepatica. hepatic fasciolosis occurs throughout the world, where climatic conditions are suitable for the survival of aquatic intermediate host snails. also of importance for ruminants, in some parts of the world, are fasciola ... | 2012 | 22521973 |