Publications

TitleAbstractYear
Filter
PMID
Filter
experimental infection of nigerian sheep and goats with bovine virus diarrhoea virus.apart from leucopenia and a low grade pyrexia nigerian goats showed no ill effects following inoculation with bovine virus diarrhoea virus. sheep showed a variable leucopenia without pyrexia. no difficulty should arise in differentiating bvd infections from peste des petits ruminants.1977202049
serological studies with the virus of peste des petits ruminants in nigeria.it was possible to distinguish separate serological responses when experimental goats were inoculated with either rinderpest virus or peste des petits ruminants virus. examination of field samples established that peste des petits ruminants occur commonly in nigerian sheep and goats although some villages have escaped recent infection. there was no evidence of infection with rinderpest in these animals.1979262608
experimental infection of domestic pigs with the virus of peste des petits ruminants.pigs could be subclinically infected with pprv by inoculation or contact with infected goats. there was no evidence that the virus could spread to uninfected pigs or goats and pigs are not considered important in the epidemiology of pprv.1979462558
a competitive elisa using anti-n monoclonal antibodies for specific detection of rinderpest antibodies in cattle and small ruminants.a competitive elisa (c-elisa) using monoclonal antibodies (mabs) which bind to the nucleo-protein (np) of rinderpest virus (rpv) for detection of rpv antibodies in cattle and small ruminant sera is described. unlike virus neutralisation test (vnt), this test using mab ivb2-4, can detect specific rpv antibodies without showing a cross-reaction with antibodies to peste-des-petits ruminants-virus (pprv); by contrast, when mab ve4-1 is used the test detects both rpv and pprv antibodies, including lo ...19921626365
serological evidence of peste des petits ruminants in jordan. 19912024417
the epidemiology of peste des petits ruminants in the sultanate of oman.virological and serological evidence was obtained to show that peste des petits ruminants virus was widely distributed in omani sheep and goats. there was no evidence for the concomitant presence of rinderpest virus in these species. two virus isolates were classified as peste des petits ruminants virus on the basis of their pathogenicity in experimental animals and their specific hybridisation with nucleic acid probes. however, neutralisation tests and polyacrylamide gel analysis of their nucle ...19902114052
pathogenicity of attenuated peste des petits ruminants virus in sheep and goats.progressive loss of virulence for goat kids was noticed when peste des petits ruminants (ppr) virus was passaged in vero cells. while goats inoculated with the 60th passage suffered from the clinical ppr disease and mortality, goats inoculated with the 80th passage did not show any sign of the disease. if the progressive loss of virulence of the virus with passage continues, it will not be long before a homologous ppr vaccine will be obtained at the national veterinary institute, vom.19902263739
microneutralisation systems for use with different strains of peste des petits ruminants virus and rinderpest virus.comparative studies were made to determine the most suitable microtitration system for assaying strains of peste des petits ruminants virus (pprv) and rinderpest virus (rv). infectivity titres did not differ significantly when assayed in either calf kidney, sheep kidney or vero cells. however, cytopathic effects were much easier to detect in the latter making them the cell of choice. addition of small amounts of virus to preformed cell monolayers in microplates with the subsequent addition of ma ...19852411037
[attenuation of a strain of rinderpest virus: potential homologous live vaccine].peste des petits ruminants (ppr) is a highly contagious disease of small ruminants frequently associated with severe mortality in these hosts. in countries where it occurs, ppr represents an important constraint to the improved productivity of sheep and goats. until now the only way to combat this plague has been the use of heterologous rinderpest vaccine; all attempts to develop a homologous vaccine have ended in failure. the present communication describes the attenuation of the nigerian strai ...19892485537
peste des petits ruminants of sheep in india. 19892609485
differentiation of rinderpest and peste des petits ruminants viruses using specific cdna clones.the morbilliviruses which infect ruminants, rinderpest (rpv) and peste des petits ruminants (pprv), are difficult to distinguish serologically. they can be distinguished by differential neutralisation tests and by the migration of the major virus structural protein, the nucleocapsid protein, on polyacrylamide gels. both these methods are time consuming and require the isolation of live virus for identification; they are not suitable for analysis of material directly from post-mortem specimens. w ...19892723018
the molecular biology of the morbillivirus (measles) group.the morbilliviruses are a closely related group of important human and animal pathogens. the best known members of the group are measles virus in man and canine distemper virus in dogs. the group also includes two other serious animal diseases, rinderpest or cattle plague and peste des petits ruminants in sheep and goats. the latter viruses are of great economic importance in africa, asia and the middle east. persistence of these viruses in some form is a possible mechanism whereby life-long imm ...19873332767
the differential growth of virulent and avirulent strains of rinderpest virus in bovine lymphocytes and macrophages.rinderpest virus (rv) grew readily in cultures of purified bovine peripheral blood lymphocytes and udder macrophages. the growth of three strains of rv was compared and there appeared to be a relationship between increasing virulence and increased ability to infect lymphocytes and macrophages. the proportion of infected cells as determined by the presence of virus antigens was a better indicator of affinity between a strain and cell type than production of new infectious virus. rv grew better in ...19853873515
a diseases of sokoto goats resembling "peste des petits ruminants". 19724366855
isolation of peste des petits ruminants virus from the sudan.based on clinical signs, the presence of a rinderpest related antigen and the ability of rinderpest immune serum to prevent virus isolation, rinderpest was diagnosed in two outbreaks in goats in central sudan during 1971 and 1972. two viruses isolated from these goats have been re-examined both serologically and by the inoculation of experimental cattle, sheep and goats. each is now considered to represent the virus of peste des petits ruminants, thereby extending the geographical range of this ...19846200906
serological studies with peste des petits ruminants and rinderpest viruses in nigeria.one hundred and ninety-five goat and 67 sheep sera collected from various parts of southern nigeria were screened for neutralising antibodies to both the peste des petits ruminants (ppr) and rinderpest viruses. neutralising antibodies against both viruses were found in the sheep and goat sera examined. parallel titration of samples which neutralised both viruses indicated a primary infection with the ppr virus (pprv). however, some samples which failed to neutralise pprv neutralised the rinderpe ...19846485096
development of a competitive elisa for detecting antibodies to the peste des petits ruminants virus using a recombinant nucleoprotein.a competitive elisa based on the reaction between a monoclonal antibody (mab) and a recombinant nucleoprotein of the peste des petits ruminants virus (pprv) was developed. this protein was obtained in large quantities from insect cells infected with a ppr nucleoprotein recombinant baculovirus (n-b). the competitive elisa was compared with the virus neutralisation test (vnt) for detecting specific antibodies to pprv in sheep and goats. the time consuming vnt is the only prescribed test that is ca ...19957709061
peste des petits ruminants antibodies in east africa. 19957754596
peste des petits ruminants in ethiopian goats.an outbreak of disease characterised by fever, ocular and nasal discharge, coughing and sneezing, oral necrosis, diarrhoea, enteritis and pneumonia in goats was shown by the use of specific cdna probes to have been peste des petits ruminants, confirmed for the first time in ethiopia. both morbidity and mortality rates were high in goats but sheep were not affected.19947941031
the detection of antibodies against peste des petits ruminants virus in cattle, sheep and goats and the possible implications to rinderpest control programmes.monoclonal antibody-based competitive elisa (c-elisa) have been used for the specific measurement of antibodies to both rinderpest and peste des petits ruminants (ppr) viruses in cattle, sheep and goats. examination of serum samples from sheep and goats in gambia, before and after vaccination with rinderpest vaccine, suggested that antibodies to ppr virus could prevent an immune response to the rinderpest vaccine. cattle sera from nigeria and ghana showed a high prevalence of antibody against pp ...19948119361
protection of goats against peste des petits ruminants with a vaccinia virus double recombinant expressing the f and h genes of rinderpest virus.peste des petits ruminants (ppr) is a viral disease of goats and sheep characterized by necrotizing and erosive stomatitis, enteritis and pneumonia. the causative agent, pprv, is a member of the family paramyxoviridae and the genus morbillivirus. other members of the genus include rinderpest (rpv), measles, canine distemper and phocid distemper viruses. ppr has a very high rate of morbidity and mortality, and effective control of this disease is of economic importance in africa, asia and the mid ...19938212844
rinderpest and peste des petits ruminants-like disease in the al-ain region of the united arab emirates.the author demonstrates that rinderpest prevailed among the cattle population in the al-ain region of the united arab emirates from 1987 to 1989. a total of twenty-four outbreaks of rinderpest were recorded during the study period. similarly, evidence of the peste des petits ruminants (ppr) or a ppr-like disease was regularly encountered in small ruminants in a total of forty-one outbreaks.19938219335
monoclonal antibody-based blocking enzyme-linked immunosorbent assay for specific detection and titration of peste-des-petits-ruminants virus antibody in caprine and ovine sera.a blocking enzyme-linked immunosorbent assay (b-elisa), using two neutralizing monoclonal antibodies (mabs), was established and compared with the virus neutralization test (vnt) for detecting specific peste-des-petits-ruminants virus (pprv) antibody in caprine and ovine sera. this technique was developed because vnt, the only available specific serological test for pprv and the cross-reactive rinderpest virus (rpv), is time-consuming and unaffordable for most laboratories in regions where both ...19938501207
recent developments in the diagnosis of rinderpest and peste des petits ruminants.effective implementation of control measures for rinderpest and peste des petits ruminants requires that a proper and rapid diagnosis of the disease is made. peste de petits ruminants (ppr) can be confused clinically with other infections such as pasteurellosis or contagious ecthyma. rinderpest, in its classical form, is easy to identify clinically; however, mass vaccination in many countries and also the emergence of mild strains of the virus have made clinical diagnosis more difficult. clinica ...19958588326
evaluation of polymerase chain reaction for the detection and characterisation of rinderpest and peste des petits ruminants viruses for epidemiological studies.the high sequence variability found in rna viruses makes it difficult to design primers for reverse transcription-polymerase chain reaction amplification which will be certain to work with all new field isolates. to overcome this problem for the detection and differential diagnosis of rinderpest (rp) and peste des petits ruminants (ppr) viruses (v), we have designed several sets of primers, based on well-conserved sequences in the p and f genes. analysis of a large number of field isolates from ...19958837881
geographic distribution and epidemiology of peste des petits ruminants virus.peste des petits ruminants (ppr) is an important viral disease of goats and sheep prevalent in west africa and the middle east. in recent years, ppr has emerged in india, first in the south india and later in north india. to study the genetic relationships between viruses of distinct geographical origin we have sequenced a 322 nucleotide cdna fragment of the fusion protein gene generated using reverse transcription followed by polymerase chain reaction (pcr) amplification. viruses from nineteen ...19968864204
the isolation of peste des petits ruminants virus from northern india.the aetiological agent responsible for an epizootic of a rinderpest-like disease afflicting sheep and goats in three states of northern india was confirmed as peste des petits ruminants virus. to differentiate the virus from rinderpest a number of diagnostic tests were used, including immunocapture elisa, specific oligonucleotide primers in a reverse transcriptase polymerase chain reaction, immunofluorescence with virus specific monoclonal antibodies and virus isolation. the virulence profile of ...19968870184
abattoir survey of sheep and goats in the gambia.an abattoir survey of sheep and goats was carried out in the gambia for one year. a total of 1248 goats and 438 sheep, predominantly young females, were slaughtered and sampled. sixty per cent of the females of both species were pregnant. there were no significant differences between the dressing percentages of different breeds and age groups. sex and stage of pregnancy had a significant influence on carcase yields in both species. in goats the highest carcase yields were obtained during the ear ...19989569483
immunohistochemical detection of peste des petits ruminants viral antigen in formalin-fixed, paraffin-embedded tissues from cases of naturally occurring infection. 199910353363
serological evidence of the absence of peste des petits ruminants in tanzania. 200010819135
improved technique for transient expression and negative strand virus rescue using fowlpox t7 recombinant virus in mammalian cells.the suitability of recombinant t7 polymerase produced using either the highly attenuated mva strain of vaccinia (mva-t7) or fowlpox virus (fp-t7) for transient expression and negative strand virus rescue was compared in two mammalian cell lines (mdbk and vero) and in primary cells of bovine, ovine and caprine origin. such primary cells are more permissive for the growth of wild type strains of morbilliviruses, such as rinderpest virus and peste des petits ruminants virus. mva-t7 was found to be ...200010996645
development and evaluation of a monoclonal antibody based competitive enzyme-linked immunosorbent assay for the detection of rinderpest virus antibodies.a competitive enzyme-linked immunosorbent assay has been standardised for the detection of antibodies to rinderpest virus in sera from cattle, sheep and goats. the test uses a neutralising monoclonal antibody (mab) directed against the haemagglutinin protein of rinderpest virus. the test is specific for rinderpest, as it failed to detect antibodies to peste des petits ruminants virus in convalescent goat sera. a 45% inhibition of the binding of the mab to the antigen was used as the cut-off poin ...200011107618
epizootiological aspects of peste des petits ruminants and rinderpest in sheep and goats in saudi arabia.epizootiological aspects of peste des petits ruminants (ppr) and rinderpest in sheep and goats in saudi arabia are examined. the presence of ppr has been suspected on occasions, but virus isolation has been successful only once. information regarding ppr and rinderpest in sheep and goats in saudi arabia is scarce. the only survey conducted indicated that neither disease is endemic in the country.200011107629
immune responses in goats to recombinant hemagglutinin-neuraminidase glycoprotein of peste des petits ruminants virus: identification of a t cell determinant.peste des petits ruminants virus (pprv), a member of the genus morbillivirus within the family paramyxoviridae, causes a fatal disease 'peste des petits ruminants' in goats and sheep. this enveloped virus is antigenically closely related to rinderpest virus (rpv), which causes a similar but distinct disease in large ruminants. pprv harbors two major surface glycoproteins, the hemagglutinin-neuraminidase (hn) and the fusion (f) proteins. the surface glycoproteins of morbilliviruses are highly imm ...200111535334
comparison of virus isolation and polymerase chain reaction for diagnosis of peste des petits ruminants.oculonasal swabs and tissue samples collected from peste des petits ruminants (ppr) suspected sheep and goats were tested for presence of the virus of peste des petits ruminants (pprv) or its rna by reverse transcription-pcr (rt-pcr) and virus isolation (vi). of 44 samples 31.8% and 40.9% were positive by vi and rt-pcr, respectively. the rt-pcr-positive samples were subjected to the nested pcr. three of six samples positive by rt-pcr but negative by vi were negative by the nested pcr. the specif ...200111774895
prevalence, distribution, and host range of peste des petits ruminants virus, turkey.peste des petits ruminants virus (pprv, genus morbillivirus), which causes a severe disease in sheep and goats, has only recently been officially declared to be present in turkey. we carried out a study to determine the prevalence, distribution, and host range of pprv in turkey. a total of 1,607 animals, reared in 18 different locations, were monitored for the presence of antibodies to pprv and the related virus of large ruminants, rinderpest virus (rpv). only two farms had animals that were fre ...200212095439
recent epidemiology of peste des petits ruminants virus (pprv).peste des petits ruminants (ppr) is an economically important viral disease of goats and sheep first described in west africa in the 1940s. the virus has been circulating in parts of sub-saharan africa for several decades and in the middle east and southern asia since 1993, although the first description of the virus in india dates to 1987. to study the genetic relationship between isolates of distinct geographical origin, a selected region of the fusion (f) protein gene of the viruses was ampli ...200212135634
goat immune response to capripox vaccine expressing the hemagglutinin protein of peste des petits ruminants.sheep-pox and capripox are contagious diseases of domestic small ruminants for which the causal agent is a poxvirus classified into the capripoxvirus genus. viruses of this group have a host range specific to sheep, goats, cattle, and possibly buffalo. thus, they are clearly indicated as vectors for the development of recombinant vaccines for peste des petits ruminants (ppr). here we report the immune response of goats inoculated with a recombinant capripox-ppr hemagglutinin.200212381569
detection of antibodies against peste des petits ruminants virus in sera of cattle, camels, sheep and goats in sudan.detection of antibodies against peste des petits ruminants virus in sera of cattle, camels, sheep and goats in sudan.200212416868
mapping of b-cell epitopic sites and delineation of functional domains on the hemagglutinin-neuraminidase protein of peste des petits ruminants virus.a recombinant baculovirus expressing membrane bound form of hemagglutinin-neuraminidase (hn) protein of peste des petits ruminants virus (pprv) was employed to generate monoclonal antibodies (mabs) against pprv-hn protein. four different mabs were employed for mapping of regions on hn carrying b-cell epitopes using deletion mutants of pprv-hn and rpv-h proteins expressed in escherichia coli as well as pprv-hn deletion proteins expressed transiently in mammalian cells. the immuno-reactivity patte ...200212457972
monoclonal antibody-based competitive elisa for simultaneous detection of rinderpest virus and peste des petits ruminants virus antibodies.an experimental competitive enzyme-linked immunosorbent assay (morbillivirus celisa) using a recombinant n antigen (rrpv n) expressed in a baculovirus and a ruminant morbillivirus (rpv and pprv)-specific monoclonal antibody (p-13a9) was developed for simultaneous detection of rinderpest virus (rpv) and peste des petits ruminants virus (pprv) antibodies and its diagnostic performance was evaluated. a set of known reference antisera against rpv and pprv belonging to different lineages, experimenta ...200314516703
development of a monoclonal antibody based competitive-elisa for detection and titration of antibodies to peste des petits ruminants (ppr) virus.peste des petits ruminants (ppr) is an acute febrile, viral, disease of small ruminants with great economic importance. a competitive-elisa (c-elisa) test was developed for detection of antibodies to ppr virus in the sera samples of goats and sheep. the test uses monoclonal antibody to a neutralizing epitope of haemagglutinin protein of the virus. based on the distribution of known negative sera samples (n=933) in respect of ppr virus antibodies in the test, a cut-off value was set as 38%. this ...200414738776
pathological and immunohistochemical study of experimental peste des petits ruminants virus infection in goats.peste des petits ruminants (ppr) is an emerging, economically important viral disease of goats and sheep in the indian subcontinent. in the present investigation, 15 hill goats were experimentally infected with 2 ml of 10% splenic suspension of a virulent isolate of ppr virus (ppr/izatnagar/94) that had caused heavy mortality (>75%) in goats during 1994 outbreaks in northern india. more than 86% (13 of 15) animals died between 9 and 13 days post inoculation at the height of temperature or when t ...200415228548
characteristic and non-characteristic pathological findings in peste des petits ruminants (ppr) of sheep in the ege district of turkey.this report describes the pathological and immunohistochemical findings in naturally infected lambs from three outbreaks of peste des petits ruminants in mugla and aydin provinces of the ege district of turkey. at necropsy, ulcerative stomatitis, catarrhal or fibrinous bronchopneumonia, and acute catarrhal enteritis were observed. histopathologically, syncytial cells containing inclusion bodies were seen in the tongue and in the buccal, labial and soft palate mucosae. in pneumonic lungs, syncyti ...200415276853
a serological survey of ruminant livestock in kazakhstan during post-soviet transitions in farming and disease control.the results of a serological survey of livestock in kazakhstan, carried out in 1997--1998, are reported. serum samples from 958 animals (cattle, sheep and goats) were tested for antibodies to foot and mouth disease (fmd), bluetongue (bt), epizootic haemorrhagic disease (ehd), rinderpest (rp) and peste des petits ruminants (ppr) viruses, and to brucella spp. we also investigated the vaccination status of livestock and related this to changes in veterinary provision since independence in 1991. for ...200415663081
full genome sequence of peste des petits ruminants virus, a member of the morbillivirus genus.peste des petits ruminants virus (pprv) causes an acute febrile illness in small ruminant species, mostly sheep and goats. pprv is a member of the morbillivirus genus which includes measles, rinderpest (cattle plague), canine distemper, phocine distemper and the morbilliviruses found in whales, porpoises and dolphins. full length genome sequences for these morbilliviruses are available and reverse genetic rescue systems have been developed for the viruses of terrestrial mammals, with the excepti ...200515845262
prevalence and distribution of peste des petits ruminants virus infection in small ruminants in india.peste des petits ruminants (ppr) is an acute febrile viral disease of goats and sheep characterised by mucopurulent nasal and ocular discharges, necrotising and erosive stomatitis, enteritis and pneumonia. the disease is endemic in india and causes large economic losses each year due to the high rates of mortality and morbidity in infected sheep and goats. the present study reports observations from 58 laboratory confirmed outbreaks of ppr and provides details of the prevalence of antibodies to ...200415861876
antibody seroprevalences against peste des petits ruminants (ppr) virus in camels, cattle, goats and sheep in ethiopia.a questionnaire-survey data indicated that 26% of 276 farmers reported the presence of respiratory disease in their herds in 2001. the incidence was perceived as "high" in small ruminants and camels, but as "low" in cattle. simultaneously, 2815 serum samples from camels (n=628), cattle (n=910), goats (n=442) and sheep (n=835) were tested. the peste des petits ruminants (ppr) antibody seroprevalence was 3% in camels, 9% in cattle, 9% in goats and 13% in sheep. the highest locality-specific seropr ...200515967242
comparison of diagnostic efficacy of a monoclonal antibody-based competitive elisa test with a similar commercial test for the detection of antibodies to peste des petits ruminants (ppr) virus. 200616437308
control of peste des petits ruminants and poverty alleviation?peste des petits ruminants (ppr) is a contagious and often fatal viral disease of sheep and goats and also wild small ruminants. the ppr virus is distinct from but closely related to rinderpest virus and both belong to the morbivillivirus genus within the family paramyxoviridae. ppr is a contagious transboundary disease with a significant impact on rural poor farmers. its control should therefore be considered in programs that aim at alleviating poverty in developing countries.200617123358
foot and mouth disease, rinderpest, peste des petits ruminants and avian influenza in the erbil, dohuk, and sulaimania governorates of iraq.foot and mouth disease (fmd) outbreaks in the northern part of iraq have been reported. the outbreaks in 1998/1999 were due to type o virus and the disease was associated with significant mortalities especially in young calves and lambs. furthermore, 10 fmd-like incidents in cattle were reported in 2002 in the three northern governorates. however, these cases were not associated with mortalities and the disease remained localized in the primary foci. type a virus was isolated from the tested sam ...200617123362
the threat of peste des petits ruminants: progress in vaccine development for disease control.peste des petits ruminants (ppr) is a highly contagious animal disease caused by a virus in the genus morbillivirus, family paramyxoviridae. this infection is responsible for high morbidity and mortality in sheep and goats and in some small wild ruminant species. the huge number of small ruminants, which are reared in the endemic areas makes ppr a serious disease threatening the livelihood of poor farmers. taking advantage of the closely relationship between rinderpest and ppr viruses, the atten ...200717399862
natural peste des petits ruminants virus infection: novel pathologic findings resembling other morbillivirus infections.the present study describes pathologic and virologic findings in 15 sheep and 6 goats that died of natural peste des petits ruminants virus infection in turkey. pathologic findings included erosive-ulcerative stomatitis, fibrino-necrotic tracheitis, bronchointerstitial pneumonia, multifocal coagulation necroses in the liver, and severe lymphocytolysis in lymphoid tissues. syncytial cells were conspicuous, especially in the oral mucosa, pulmonary alveoli, liver, and lymphoid tissues. in addition ...200717606509
development of 2 types of competitive enzyme-linked immunosorbent assay for detecting antibodies to the rinderpest virus using a monoclonal antibody for a specific region of the hemagglutinin protein.a competitive enzyme-linked immunosorbent assay (c-elisa) has been developed and standardized for the detection of antibodies to the rinderpest virus (rpv) in sera from cattle, sheep, and goats. the test is specific for rinderpest because it does not detect antibodies to peste-des-petits-ruminants virus (pprv). the test depends on the ability of the monoclonal antibody (mab) directed against the hemagglutinin (h) protein of rpv to compete with the binding of rpv antibodies in the positive serum ...200717668032
detection of peste des petits ruminants virus antigen using immunofiltration and antigen-competition elisa methods.peste des petits ruminants (ppr) is one of the most economically important diseases affecting sheep and goats in india. an immunofiltration-based test has been developed using either mono-specific serum/monoclonal antibodies (mab) prepared against a recombinant truncated nucleocapsid protein of rinderpest virus (rpv) cross-reactive with ppr virus. this method consists of coating ocular swab eluate from suspected animals onto a nitrocellulose membrane housed in a plastic module, which is allowed ...200818182256
development of one-step real-time rt-pcr assay for detection and quantitation of peste des petits ruminants virus.in this study, a rapid and specific taqman-based, one-step real-time quantitative reverse transcription pcr (qrt-pcr) has been described for the detection of peste des petits ruminants virus (pprv). primers and probe were designed based on the nucleocapsid protein gene sequence. the real-time qrt-pcr assay was able to detect pprv isolates from very distinct geographical areas (africa, middle east and asia). the specificity of the assay was assessed by including rinderpest virus and other morbill ...200818243345
seroprevalence of, and risk factors for, peste des petits ruminants in sheep and goats in northern jordan.peste des petits ruminants (ppr) is an economically important disease that affect sheep and goat industry in asia and africa. in this study, we investigated the seroprevalence, and risk factors, of ppr in sheep and goat flocks from five different governorates (irbid, jarash, ajloun, mafraq and zarka) located in northern jordan. serum samples from 929 and 400 sheep and goats, respectively, corresponding to 122 sheep flock and 60 goats flock were collected. seroprevalence was determined using ppr ...200818291541
concurrent peste des petits ruminants virus and pestivirus infection in stillborn twin lambs.concurrent infection with peste des petits ruminants virus (pprv) and pestivirus was diagnosed in stillborn twin lambs. with the flock history, the findings of epidermal syncytial cells and necrotizing bronchitis/bronchiolitis prompted testing for pprv infection, and pprv antigen was detected by immunohistochemistry (ihc) in the skin, lungs, kidneys, rumen, and thymus. macroscopic anomalies that were typical of border disease included scoliosis, brachygnathism, prognathism, arthrogryposis, hydra ...200818424832
rapid detection of peste des petits ruminants (ppr) virus antigen in sudan by agar gel precipitation (agpt) and haemagglutination (ha) tests.agpt and ha tests were employed for rapid diagnosis of pprv infection in sheep and goats in sudan. forty lymph nodes and spleen samples from suspected cases of ppr in both sheep and goats were examined by agpt and ha tests for detection of pprv antigen. viral antigen was detected from (77.5%) of the samples tested by agpt and (92.5%) tested by ha test. the results of both tests revealed that ha test was more sensitive than agpt for detection of pprv antigen (kappa statistics 0.4366). another adv ...200818509945
standardization of large scale production of homologous live attenuated ppr vaccine in india.live attenuated homologous vaccine against peste des petits ruminants of sheep and goats was produced on a large scale basis in roller culture bottles using seed virus developed at the indian veterinary research institute, muktheswar, india. vero cells between 130-150 passages with six percent foetal calf serum were used for the production of vaccine. the cells were infected with 0.01 multiplicity of infection and harvested when the cytopathic effect was 80%. the vaccine was freezedried in order ...200818551773
in situ detection of ppr virus antigen in skin papules around the mouth of sheep experimentally infected with ppr virus. 200818557185
the detection of antibody against peste des petits ruminants virus in sheep, goats, cattle and buffaloes.monoclonal antibody-based competitive elisa (c-elisa) has been used for the specific measurement of antibodies to peste des petits ruminants (ppr) viruses in sheep, goats, cattle and buffalo. serum samples from sheep (n = 232), goats (n = 428), cattle (n = 43), buffalo (n = 89) were tested. the animals had not been vaccinated against rinderpest or ppr. findings suggested that the sero-positive cases were significantly higher in sheep (51.29%) than in goats (39.02%) (p = 0.002). the overall sero- ...200818716909
post-vaccination antibodies profile against peste des petits ruminants (ppr) virus in sheep and goats of punjab, pakistan.a total of 70 sheep and 330 goats were selected randomly. all the animals were kept under same housing and management conditions. serum samples were collected from all the animals and tested for the presence of antibodies against peste des petits ruminants (ppr) virus using competitive elisa (celisa). all the animals were found negative showing percentage inhibition (pi) values <50. the animals were vaccinated against ppr with nig/75/1 strain vaccine of ppr serum samples were collected from rand ...200918758987
peste des petits ruminants (ppr) in ethiopia: analysis of a national serological survey.peste des petits ruminants (ppr) is a contagious viral disease of small ruminants in africa and asia. in 1999, probably the largest survey on ppr ever conducted in africa was initiated in ethiopia where 13 651 serum samples from 7 out of the 11 regions were collected and analyzed by competitive enzyme-linked immunosorbent assay (celisa). the objective of this paper is to present the results of this survey and discuss their practical implications for ppr-endemic regions.200818786275
relationship between the level of signaling lymphocyte activation molecule mrna and replication of peste-des-petits-ruminants virus in peripheral blood mononuclear cells of host animals.basal signaling lymphocyte activation molecule (slam) expression in the peripheral blood mononuclear cells (pbmcs) of cattle, buffalo, sheep, and goats was correlated with peste-des-petits-ruminants virus (pprv) replication assessed by real-time pcr and virus titers. pbmcs from goats had the highest level of slam mrna followed by sheep, cattle, and buffalo. different breeds of goats had different basal levels of slam mrna. in the pbmcs of studied animals, basal slam expression had high correlati ...200819143479
peste des petits ruminants virus in tibet, china.serologic and molecular evidence indicates that peste des petits ruminants virus (pprv) infection has emerged in goats and sheep in the ngari region of southwestern tibet, people's republic of china. phylogenetic analysis confirms that the pprv strain from tibet is classified as lineage 4 and is closely related to viruses currently circulating in neighboring countries of southern asia.200919193278
seroepidemiology of peste des petits ruminants in sheep and goats of southern peninsular india.this paper presents the results of a seroepidemiological study carried out between july 2006 and march 2007 to detect the presence of antibodies to peste des petits ruminants (ppr) virus in randomly collected serum samples from sheep and goats in southern peninsular india. the authors used a competitive enzyme-linked immunosorbent assay with a monoclonal antibody developed against a neutralising epitope of the haemagglutinin (ha) protein of the virus. a total of 1,492 sheep sera and 2,068 goat s ...200819284053
the epidemiology of peste des petits ruminants in pakistan and possible control policies.peste des petits ruminants made its first clinical appearance in pakistan during an epidemic in punjab province in 1991, but its level of activity in subsequent years was never systematically recorded. in 2003, 2004 and 2005 teams trained in participatory disease surveillance methods visited a large number of villages throughout pakistan to record the prevalence and impact of key livestock diseases. peste des petits ruminants has emerged from their study as a common and economically damaging dis ...200819284054
the epidemiology of peste des petits ruminants in pakistan.peste des petits ruminants (ppr) is an acute and highly contagious viral disease of small ruminants, which is newly emerging in pakistan. information provided by participatory disease surveillance teams was used to develop a database for ppr outbreaks in pakistan. twenty-four villages were selected throughout the country and field investigations were conducted in each village to study the dynamics of the disease. in each area, flocks with a history of ppr were identified and serological sampling ...200819284055
current situation of peste des petits ruminants (ppr) in the sudan.the current situation of ppr in sudan was investigated. a total of 61 tissue samples were collected from various ppr suspected outbreaks in sheep in sudan during 2008. collected tissue samples were tested for ppr antigen using icelisa, ppr antigen was detected in 26 out of 61 samples (42.6%). highest antigen detection rate was in specimens collected from western sudan. a total of 1198 serum samples were collected from sheep (n = 500), camels (n = 392), and goats (n = 306) from different areas in ...201019548103
a serologic investigation for peste des petits ruminants infection in sheep, cattle and camels (camelus dromedarius) in aydin province, west anatolia. 201019554466
antibody seroprevalences against peste des petits ruminants (ppr) virus in sheep and goats in sudan.counter immnuo-electrophoresis (ciep) and competitive elisa (c-elisa) tests were employed for seroprevalence of peste des petits ruminants (ppr) infection in sudan. the result of both tests showed high prevalence of pprv antibodies in sheep and goats sera collected from six different regions of sudan. of the 519 serum samples examined for the presence of pprv antibodies 307(59.15%) were positive by ciep while 263(50.67%) were positive by c-elisa. ciep technique was shown to be more sensitive tha ...200919798772
quantitative one-step real-time rt-pcr for the fast detection of the four genotypes of pprv.a one-step real-time taqman rt-pcr assay (rrt-pcr) for peste des petits ruminants virus (pprv) was developed to detect the four lineages of pprv by targeting the nucleoprotein (n) gene of the virus. this new assay was compared to a conventional rt-pcr on reference strains and field materials. quantitation was performed against a standard based on a synthetic transcript of the nppr gene for which a minimum of 32 copies per reaction were detected with a corresponding c(t) value of 39. depending on ...201020116402
vaccination for the control of peste des petits ruminants. 200415742621
economics of prophylaxis against peste des petits ruminants and gastrointestinal helminthosis in small ruminants in north cameroon.data on reproduction and mortality were collected over one year from 5100 sheep and 13,300 goats in treated and control flocks. the treated animals received vaccination against peste des petits ruminants (ppr) and anthelmintics twice a year. productivity parameters (fecundity and mortality rates) obtained with and without prophylaxis were fitted into a benefit-cost economic analysis model and run for project lifespans varying from one to five years. at a 7% discount rate, the overall benefits fo ...200011147279
status of sheep sera to bluetongue, peste des petits ruminants and sheep pox in a few northern states of india.bluetongue (bt), peste des petits ruminants (ppr) and sheep pox are the most economically important viral diseases of sheep in india. serum samples obtained from sheep in five northern states of the country were screened for antibody against these agents to explore the extent of spread of these infections. a total of 516 serum samples were screened for the presence of antibodies against bt and ppr viruses. of these, 155 samples were also tested for antibodies against sheep pox virus. bt antibodi ...200820405449
a short note on peste des petits ruminants in karnataka, india.retrospective quantitative analysis of epidemiological data on peste des petits ruminants (ppr) from the department of animal husbandry and veterinary sciences in karnataka, southern india, revealed significant information about the disease in this area. in the nine years between april 1998 and march 2007 a total of 624 outbreaks were reported in the state. with the exception of the 12-month period between april 2001 and march 2002 the disease occurred every year. the study shows clearly that in ...200920462160
a goat poxvirus-vectored peste-des-petits-ruminants vaccine induces long-lasting neutralization antibody to high levels in goats and sheep.recombinant capripoxvirus (cpv) is a promising candidate differentiating infected from vaccinated animals (diva) vaccine against peste-des-petits-ruminants (ppr). in order for recombinant cpv to be successfully used in the field, there should exist dependable indicators for quality control of vaccine products, surveillance and vaccination evaluation. viral neutralization antibody (vna) is correlated to protection against ppr and is a technically feasible indicator for this purpose. the immunogen ...201020471441
detection and genetic characterization of peste des petits ruminants virus in free-living bharals (pseudois nayaur) in tibet, china.peste des petits ruminants (ppr) is an important viral disease of sheep and goats. the wildlife hosts of ppr, which may play an important role in the epidemiology of this disease, are not well characterized. the research was undertaken to study the infection of ppr virus (pprv) in free-living bharals (pseudois nayaur) in tibet, china. in 2007, pprv infection was confirmed in two bharals in rutog county of tibet based on clinical signs and detection of pprv rna in tissue samples. in 2008, pprv in ...201120591454
cloning and expression of the nucleoprotein of peste des petits ruminants virus in baculovirus for use in serological diagnosis.peste des petits ruminants (ppr) is a viral disease of goats and sheep characterized by erosive stomatitis, enteritis, and pneumonia. the virus is a member of the family paramyxoviridae and the genus morbillivirus. the disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. we have cloned and sequenced the cdna of the nucleocapsid (n) gene of the nigeria 75/1 strain of ppr virus (pprv). a comparison of its nucleotide and deduced amino acid se ...19957747450
an outbreak of peste des petits ruminants in a zoological collection.peste des petits ruminants virus was suspected to be the cause of a disease outbreak in a zoological collection at al ain in the arabian gulf. clinically the outbreak affected gazelles (gazellinae), ibex and sheep (caprinae) and gemsbok (hippotraginae); subclinical involvement of nilgai (tragelaphinae) was suspected. a morbillivirus was isolated and using monoclonal antibodies and biological tests in cattle, sheep and goats the virus of peste des petits ruminants was identified.19873424615
application of semi-quantitative m gene-based hydrolysis probe (taqman) real-time rt-pcr assay for the detection of peste des petits ruminants virus in the clinical samples for investigation into clinical prevalence of disease.peste des petits ruminants (ppr) is a contagious, notifiable and economically important transboundary viral disease of small ruminants. in this study, a hydrolysis probe-based real-time reverse transcription-polymerase chain reaction (rt rt-pcr) assay for the detection and semi-quantification of ppr virus (pprv) nucleic acid was developed using the virus rna and matrix (m) gene-specific primers with hex-labelled fluorescent probe and applied for the detection of pprv in clinical samples to ident ...201020723163
rapid detection of peste des petits ruminants virus by a reverse transcription loop-mediated isothermal amplification assay.peste des petits ruminants virus (pprv) is the causative agent of peste des petits ruminants (ppr), an economically important viral disease of small ruminants. in this report, a one-step, single-tube, reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for the detection of pprv. a set of six lamp primers were designed based on the matrix gene sequence of pprv to amplify the target rna by incubation at 63°c for 60min with bst dna polymerase and reverse trans ...201020813134
experimental peste des petits ruminants (goat plague) in goats and sheep.in order to study the pathomorphology and immunohistochemistry of peste des petits ruminants, four goats and two sheep were inoculated intranasally with the malig-yemen strain of peste des petits ruminants virus. the animals developed fever, nasal discharge, oral erosions, cough and diarrhea. one goat and one sheep died and one moribund goat was killed. three animals survived the infection. at necropsy, erosive stomatitis, pneumonia and gastroenteritis were found. histopathologically the pneumon ...19883280108
reverse genetics for peste-des-petits-ruminants virus (pprv): promoter and protein specificities.peste-des-petits-ruminants virus (pprv) (family paramyxoviridae, genus morbillivirus) causes an acute febrile illness in sheep and goats resulting in significant morbidity and mortality in infected herds. the paramyxoviruses all have negative sense, non-segmented rna genomes and their host range and pathogenic determinants have been extensively studied using reverse genetics. this technology also enables a more rational approach to be taken with respect to vaccine design. in order to initiate th ...200717350130
evaluation of peste des petits ruminants cell culture vaccine in sheep and goats in pakistan.the authors study the antibody response of a locally prepared live-attenuated peste des petits ruminants (ppr) cell culture vaccine in sheep and goats. antibodies were measured using the competitive enzyme-linked immunosorbent assay. the vaccine was found to be safe and produced high serological titres within 21 days post vaccination. the serological titres remained high for one year post vaccination.201020857380
post vaccination and colostral peste des petits ruminants antibody dynamics in research flocks of kirdi goats and foulbe sheep of north cameroon.we studied kirdi goats and foulbe sheep kept on-station at the institute of agricultural research for development (irad), garoua to look at their immunological response following vaccination with a specific peste des petits ruminant (ppr) virus vaccine. pre-vaccination ppr antibody seroprevalences were 44 and 29% in goats and sheep, respectively. seroprevalences following vaccination were 100% in both species. titres remained above the protection threshold level (1:10) in both species at 12 mont ...200212392877
comparative efficacy of standard agid and precipitinogen inhibition test with monoclonal antibodies based competitive elisa for the serology of peste des petits ruminants in sheep and goats.this project was conducted to investigate the comparative efficiency of competitive elisa (celisa), standard agar gel immunodiffusion test (agid) and precipitinogen inhibition test (pit) for the diagnosis of peste des petits ruminants (ppr) in pakistan. to deal with this, serum samples from 198 sheep and 82 goats were collected from three different government livestock farms and all the samples were run simultaneously with the three serological tests. the samples found positive for ppr antibodie ...200918622735
evidence of peste des petits ruminants virus (pprv) infection in sindh ibex (capra aegagrus blythi) in pakistan as confirmed by detection of antigen and antibody.an outbreak resulting in mortality in sindh ibex (capra aegagrus blythi) was investigated. there was a history of about 36 deaths (both young and adult) during the period of 1 month. disease appeared in a generalized form, affecting the respiratory and digestive systems. major lesions were respiratory distress, pustules on and in the mouth, ocular-nasal discharges, and severe diarrhea. the most significant lesion was the oculonasal discharges and diarrhea. deaths were mainly due to blindness, an ...201121221782
exotic animal diseases bulletin. peste des petits ruminants. 201021222301
genetic characterization of indian peste des petits ruminants virus (pprv) by sequencing and phylogenetic analysis of fusion protein and nucleoprotein gene segments.peste des petits ruminants (ppr) is an important viral disease of sheep and goats, endemic in india. the study was undertaken to characterize the local pprv by sequencing fusion (f) protein and nucleoprotein (n) gene segments and phylogenetic analysis, so as to focus on genetic variation in the field viruses. selected regions of pprv genome were amplified from clinical samples collected from 32 sheep and goats by rt-pcr and the resulting amplicons were sequenced for phylogenetic analysis. the ph ...200817850836
an outbreak of peste des petits ruminants in migratory flocks of sheep and goats in egypt in 2006.an outbreak of peste des petits ruminants (ppr) was recorded in kalubia province, egypt in 2006, affecting a large population of migratory goats and sheep over a huge geographical area. epidemiological, clinical and laboratory investigations were performed. diseased animals showed pyrexia, erosive stomatitis, enteritis and bronchopneumonia. clinical manifestations were more severe in goats. the overall morbidity, cumulative mortality and case fatality rates were 26.1%, 10.5% and 40.2%, respectiv ...201021309463
rapid quality control of a live attenuated peste des petits ruminants (ppr) vaccine by monoclonal antibody based sandwich elisa.peste des petits ruminants (ppr) is a highly contagious and economically important viral disease of goats and sheep. a homologous vero cell-based attenuated ppr vaccine developed in our laboratory and used extensively throughout the country, is available for control of ppr. the presently used quality control test, titration in vero cells for ppr virus titre in vaccine batches, takes at least 6-8days to determine the quality and dose of vaccine. in this study, 74 freeze-dried ppr vaccine batches ...200817644410
peste des petits ruminants (ppr) outbreak in tajikistan.the occurrence of outbreaks of peste des petits ruminants (ppr) in three districts of tajikistan is described. the causal strain (ppr tajikistan) was characterized and the sequence of its n gene was compared with that of 43 other strains isolated since 1968 in africa, the middle east and asia. the study demonstrated (1) the value of the n gene as a target in comparing isolates obtained over an extended period of evolution, and (2) that clustering was related to the geographical origin of strains ...200717321539
asian lineage of peste des petits ruminants virus, africa.interest in peste des petits ruminants virus (pprv) has been stimulated by recent changes in its host and geographic distribution. for this study, biological specimens were collected from camels, sheep, and goats clinically suspected of having pprv infection in sudan during 2000-2009 and from sheep soon after the first reported outbreaks in morocco in 2008. reverse transcription pcr analysis confirmed the wide distribution of pprv throughout sudan and spread of the virus in morocco. molecular ty ...201121762576
differentiating pestes des petits ruminants and rinderpest viruses by a novel monoclonal antibody.peste des petits ruminant (ppr) is an acute, febrile, viral disease of small ruminants with great economic importance. ppr and rinderpest (rp) viruses are antigenically related and need to be differentiated serologically. the use of monoclonal antibodies (mabs) in elisa for specific diagnostics and separation of ppr and rpv is important. for this purpose, six balb/c mice were immunized with inactivated antigen from the nijeria strain. fusion cloning was performed 3 months later by directly using ...201121707365
pathogenicity of a local peste des petits ruminants virus isolate in sheep in turkey. 200516450860
peste des petits ruminants has been widely present in southern india since, if not before, the late 1980s.because previous authorities had suggested that small ruminants were playing a part in the dissemination of rinderpest, and a rinderpest-eradication campaign was about to begin, it was necessary to make precise virus identifications from a number of small-ruminant "rinderpest" outbreaks. when this was done using a database created from passive disease reports, we found that epidemics-reportedly due to rinderpest-were in fact due to peste des petits ruminants (pprs). although such cases had been ...200211849724
Seroprevalence and sero-conversion after vaccination against Peste des Petits Ruminants in sheep and goats from Awash Fentale District, Afar, Ethiopia.A cross-sectional epidemiological study followed by vaccination and postvaccinal serum antibody assessment against Peste des Petits Ruminants (PPR) in small ruminant population of Awash Fentale District, Afar, Ethiopia, was conducted from September 2006 to June 2007 with the aim of investigating seroprevalence and post-vaccination sero-conversion rate. A total of 1239 sera collected from sheep and goats which were not vaccinated, were screened by using nucleoprotein-based competitive enzyme-link ...201222088269
comparative efficacy of peste des petits ruminants (ppr) vaccines.peste des petits ruminants (ppr) is a highly contagious, economically important viral disease of sheep and goats with high morbidity and mortality rates. in order to control the disease effectively, highly sensitive diagnostic tests coupled with potent vaccines are important pre-requisites. at present, there are three live attenuated ppr vaccines available in india including sungri 96, arasur 87 and coimbatore 97. indian veterinary research institute (ivri) mukteswar developed the ppr sungri 96 ...201020199873
Displaying items 1 - 100 of 127