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[diagnosis of vibriosis in bulls at breeding stations through lavages of the artificial vagina].the objective of the study was to test the suitability of lavages of artificial vaginas which are used for collecting ejaculate for diagnosing the campylobacter fetus subsp. fetus germs. according to the results of the investigation this method may be considered equal to the method of lavages of the prepuce. however, it is important to point out the advantages: it eliminates the danger of an accident, it enables quality lavage in the laboratory. to caputure the campylobacter fetus the filtration ...197896573
prevalence of bovine campylobacteriosis in indigenous cattle of three states in nigeria.a survey of bovine campylobacteriosis in breeding bulls and cows was carried out in the states of kaduna, kano and borno. six hundred and eighty nine cattle composed of 585 and 104 breeding bulls and cows respectively were sampled. campylobacter fetus subsp. venerealis was isolated from 12 bulls while campylobacter fetus subsp. fetus was isolated from three of them. campylobacter fetus subsp. fetus was isolated from four cows while campylobacter fetus subsp. veneralis was isolated from one cow. ...19911763437
production of cytolethal distending toxin (cldt) by campylobacter fetus subsp. fetus isolated from calves.cytolethal distending toxin (cldt) production by campylobacter fetus subsp. fetus isolated from calves was examined using cho cells. twenty-five of the 26 strains tested were positive for cldt with titer of ranging 1:8 to 1:2,048. cldt positive strains were divided into low and high cytotoxin titer groups. isolates from diarrhea cases tended to produce a significant amount of cldt compared with isolates from liver. cldt produced by c. fetus showed no effect on y-1 cells and was heat-labile and t ...19938357933
role of s-layer protein antigenic diversity in the immune responses of sheep experimentally challenged with campylobacter fetus subsp. fetus.surface layer proteins (slps) are essential for induction of abortion by campylobacter fetus subsp. fetus in experimentally challenged ewes. these proteins are encoded by multiple sap genes and vary in size and antigenicity. the role of slp antigenic variation during experimental ovine infection was investigated. following subcutaneous challenge, the slps were highly antigenic, and antibodies were detected in serum, milk, bile, and urine. fecal anti-slp antibodies were detected only in animals c ...200312496160
investigation of bovine venereal campyloacteriosis in beef cow herds in new zealand.to determine regional prevalences of beef cow herds in new zealand positive for campylobacter fetus subsp venerealis antibodies in samples of vaginal mucus tested using an immunoglobulin (ig) a enzyme-linked immunosorbent assay (elisa), and to examine the suitability of the iga elisa for detecting infection with c. fetus subsp venerealis under field conditions in new zealand.200515731834
[campylobacter as a venereal disease in cattle].since the introduction of ai, venereal diseases caused by tritrichomonas fetus and campylobacter fetus subsp. venerealis haved been eradicated in the netherlands. campylobacter fetus subsp. fetus can cause sporadic abortion and early embryonic death. when natural breeding is practised, venereal diseases must be included in the list of differential diagnoses of fertility problems. a case study of a campylobacter fetus subsp. fetus infection is described.200516047745
a multiplex polymerase chain reaction to detect and differentiate campylobacter fetus subspecies fetus and campylobacter fetus -species venerealis: use on uk isolates of c. fetus and other campylobacter spp.subspeciation of campylobacter fetus subsp. fetus (cff) and campylobacter fetus subsp. venerealis (cfv) is important for international animal import regulations. phenotyping can be unreliable, and genotyping by techniques like pulsed field gel electrophoresis is difficult in routine diagnostic laboratories. a pcr subspeciation technique has been reported [aust vet j (1997) 75, 827]; we aimed to develop this pcr and investigate its use on uk c. fetus isolates.200516162226
molecular epidemiology of campylobacter fetus subsp. fetus on bovine artificial insemination stations using pulsed field gel electrophoresis.the presence of campylobacter fetus subspecies fetus (cff) on bovine artificial insemination (ai)-stations can have major economical consequences. more knowledge on the epidemiology of c. fetus is needed to control cff infections at ai-stations. we assessed the epidemiology of cff on ai-stations and the molecular relationship between cff strains isolated from outbreaks on ai-stations. thirteen cff strains (two cff strains per outbreak and one sporadic case) isolated from bulls housed on differen ...200616289638
emerging thermotolerant campylobacter species in healthy ruminants and swine.campylobacters other than campylobacter jejuni or c. coli were isolated in 35% of 343 farms recently analyzed in northern spain. this study was aimed at identifying at the species level the 120 isolates collected (21 ovine, 52 beef cattle, 44 dairy cattle, and 3 porcine) by species-specific polymerase chain reaction and 16s rrna gene sequencing analysis. thus, five species were identified: campylobacter hyointestinalis (90 isolates), campylobacter lanienae (12), campylobacter fetus subsp. fetus ...201121438765
genetic divergence of campylobacter fetus strains of mammal and reptile origins.campylobacter fetus is a gram-negative bacterial pathogen of both humans and animals. two subspecies have been identified, campylobacter fetus subsp. fetus and campylobacter fetus subsp. venerealis, and there are two serotypes, a and b. to further investigate the genetic diversity among c. fetus strains of different origins, subspecies, and serotypes, we performed multiple genetic analyses by utilizing random amplification of polymorphic dna (rapd), pulsed-field gel electrophoresis (pfge), and d ...200516000457
shift in s-layer protein expression responsible for antigenic variation in campylobacter fetus.campylobacter fetus strains possess regular paracrystalline surface layers (s-layers) composed of high-molecular-weight proteins and can change the size and crystalline structure of the predominant protein expressed. polyclonal antisera demonstrate antigenic cross-reactivity among these proteins but suggest differences in epitopes. monoclonal antibodies to the 97-kda s-layer protein of campylobacter fetus subsp. fetus strain 82-40lp showed three different reactivities. monoclonal antibody 1d1 re ...19937688715
immunisation against bovine vibriosis due to campylobacter fetus subsp. fetus biotype intermedius.a vaccine prepared from campylobacter fetus subsp. fetus biotype venerealis gave only partial protection to heifers and bulls against genital infection with c. fetus subsp. fetus biotype intermedius. heifers and bulls were fully immunised against c. fetus subsp. venerealis biotype intermedius by administration of a vaccine prepared from homologous organisms.1976985256
comparison of three sampling methods for the diagnosis of genital vibriosis in the bull.three different methods of collecting preputial material for bacteriological examination were compared using 3 bulls infected with campylobacter fetus subsp. fetus. the first method utilised a specially designed instrument to scrape the preputial and penile mucosa, int he second method plastic pipettes were used to aspirate material and the third method involved washing the preputial cavity with sterile peptone water. bacteriological examination of the samples showed conclusively that scraping w ...1977612318
isolation of campylobacter fetus subspecies fetus in a two-year-old quarterhorse with chronic diarrhea of an undetermined etiology.a 2-year-old quarterhorse was evaluated for chronic diarrhea and weight loss of 5 weeks duration after numerous diagnostic tests failed to identify an underlying cause. historically, the horse was housed at pasture where human household waste vehicles were routinely cleaned and the effluent could run onto the field. physical examination revealed poor body condition and frequent high-volume diarrhea. diagnostic testing for salmonella spp., endoparasites, cryptosporidium spp., clostridium spp., an ...200919286512
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