identification of prevalent potyvirus on maize and johnsongrass in corn fields of tehran province of iran and a study on some of its properties.during a growing season in 2004, 231 leaf samples of virus infected and mosaic and dwarf mosaic symptoms showing maize (zea mays l.) plants and 258 leaf samples of mosaic showing johnsongrass (sorghum halepens l.) plants from various corn fields in tehran province were collected. serological tests of das-elisa and diba were performed on samples using antisera of sugarcane mosaic virus (scmv), maize dwarf mosaic virus (mdmv), sorghum mosaic virus (srmv) and johnsongrasss mosaic virus (jgmv). in b ...200617390895
quantitative trait loci mapping of resistance to sugarcane mosaic virus in maize.abstract sugarcane mosaic virus (scmv) is an important virus disease of maize (zea mays) in europe. in this study, we mapped and characterized quantitative trait loci (qtl) affecting resistance to scmv in a maize population consisting of 219 f(3) or immortalized f(2) families from the cross of two european maize inbreds, d32 (resistant) x d145 (susceptible). resistance was evaluated in replicated field trials across two environments under artificial inoculation. the method of composite interval ...199918944678
validation of candidate genes putatively associated with resistance to scmv and mdmv in maize (zea mays l.) by expression profiling.the potyviruses sugarcane mosaic virus (scmv) and maize dwarf mosaic virus (mdmv) are major pathogens of maize worldwide. two loci, scmv1 and scmv2, have ealier been shown to confer complete resistance to scmv. custom-made microarrays containing previously identified scmv resistance candidate genes and resistance gene analogs were utilised to investigate and validate gene expression and expression patterns of isogenic lines under pathogen infection in order to obtain information about the molecu ...200919187556
isolation, expression and functional analysis of a putative rna-dependent rna polymerase gene from maize (zea mays l.).rna-dependent rna polymerases (rdrps) in plants have been reported to be involved in post-transcriptional gene silencing (ptgs) and antiviral defense. in this report, an rdrp gene from maize (zmrdrp1) was obtained by rapid amplification of cdna ends (race) and rt-pcr. the mrna of zmrdrp1 was composed of 3785 nucleotides, including a 167 nt 5' untranslated region (utr), a 291 nt 3'utr and a 3327 nt open reading frame (orf), which encodes a putative protein of 1108 amino acids with an estimated mo ...201019685166
application of sequence-independent amplification (sia) for the identification of rna viruses in bioenergy crops.miscanthus x giganteus, energycane, and panicum virgatum (switchgrass) are three potential biomass crops being evaluated for commercial cellulosic ethanol production. viral diseases are potentially significant threats to these crops. therefore, identification of viruses infecting these bioenergy crops is important for quarantine purposes, virus resistance breeding, and production of virus-free planting materials. the application is described of sequence-independent amplification, for the identif ...201020638415
responses of maize (zea mays l.) near isogenic lines carrying wsm1, wsm2, and wsm3 to three viruses in the potyviridae.genes on chromosomes six (wsm1), three (wsm2) and ten (wsm3) in the maize (zea mays l.) inbred line pa405 control resistance to wheat streak mosaic virus (wsmv), and the same or closely linked genes control resistance to maize dwarf mosaic virus (mdmv) and sugarcane mosaic virus (scmv). near isogenic lines (nil) carrying one or two of the genes were developed by introgressing regions of the respective chromosomes into the susceptible line oh28 and tested for their responses to wsmv, mdmv, and sc ...201121667271
application of rna interference methodology to investigate and develop scmv resistance in maize.specific fragments of the sugarcane mosaic virus (scmv) coat protein gene (cp) were amplified by reverse transcriptionpolymerase chain reaction and used to construct a marker free small interfering rna complex expression vector against scmv. in planta transformation was performed on maize (zea mays) inbred line 8112 mediated by agrobacterium tumefaciens. pcr and southern blot analyses demonstrated successful integration of the cp segment into the 8112 genome. the in planta transformation frequen ...201425189224
maize-pathogen interactions: an ongoing combat from a proteomics perspective.maize (zea mays l.) is a host to numerous pathogenic species that impose serious diseases to its ear and foliage, negatively affecting the yield and the quality of the maize crop. a considerable amount of research has been carried out to elucidate mechanisms of maize-pathogen interactions with a major goal to identify defense-associated proteins. in this review, we summarize interactions of maize with its agriculturally important pathogens that were assessed at the proteome level. employing diff ...201526633370
maize elongin c interacts with the viral genome-linked protein, vpg, of sugarcane mosaic virus and facilitates virus infection.the viral genome-linked protein, vpg, of potyviruses is involved in viral genome replication and translation. to determine host proteins that interact with sugarcane mosaic virus (scmv) vpg, a yeast two-hybrid screen was used and a maize (zea mays) elongin c (zmelc) protein was identified. zmelc transcript was observed in all maize organs, but most highly in leaves and pistil extracts, and zmelc was present in the cytoplasm and nucleus of maize cells in the presence or absence of scmv. zmelc exp ...201424954157
phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus.protein phosphorylation plays a pivotal role in the regulation of many cellular events. no information is yet available, however, on protein phosphorylation in plants in response to virus infection. in this study, we characterized phosphoproteomes of resistant and susceptible genotypes of maize (zea mays l.) in response to sugarcane mosaic virus (scmv) infection. based on isotope tags for relative and absolute quantification technology, tio2 enrichment method and lc-ms/ms analysis, we identified ...201525488425
characterization of small interfering rnas derived from sugarcane mosaic virus in infected maize plants by deep sequencing.rna silencing is a conserved surveillance mechanism against viruses in plants. it is mediated by dicer-like (dcl) proteins producing small interfering rnas (sirnas), which guide specific argonaute (ago)-containing complexes to inactivate viral genomes and may promote the silencing of host mrnas. in this study, we obtained the profile of virus-derived sirnas (vsirnas) from sugarcane mosaic virus (scmv) in infected maize (zea mays l.) plants by deep sequencing. our data showed that vsirnas which d ...201424819114
proteomic and phytohormone analysis of the response of maize (zea mays l.) seedlings to sugarcane mosaic virus.sugarcane mosaic virus (scmv) is an important virus pathogen in crop production, causing serious losses in grain and forage yields in susceptible cultivars. control strategies have been developed, but only marginal successes have been achieved. for the efficient control of this virus, a better understanding of its interactions and associated resistance mechanisms at the molecular level is required.201323894637
identification of differentially regulated maize proteins conditioning sugarcane mosaic virus systemic infection.sugarcane mosaic virus (scmv) is the most important cause of maize dwarf mosaic disease. to identify maize genes responsive to scmv infection and that may be involved in pathogenesis, a comparative proteomic analysis was performed using the first and second systemically infected leaves (termed 1 sl and 2 sl, respectively). seventy-one differentially expressed proteins were identified in 1 sl and 2 sl upon scmv infection. among them, eight proteins showed the same changing patterns in both 1 sl a ...201728627019
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