immunity to hantavirus challenge in meriones unguiculatus induced by vaccinia-vectored viral proteins.vaccinia virus recombinants were constructed that incorporated genomic sequences coding for the nucleoprotein (n) and glycoproteins (g1 and g2) of the hantavirus r22 strain isolated from a rat in china, and designated as rnv and rmv9, respectively. the proteins expressed by rnv and rmv9 were identified by radioimmunoprecipitation and indirect immunofluorescence assay using a panel of monoclonal antibodies and polyclonal immune sera, and were found to be antigenically indistinguishable from authe ...19921359802
hantaanvirus among urban rats from a slum area in bangkok.a total of 106 rodents sera from slum wat phai ton and slum klong toey were examined by immunofluorescent antibody assay during may to august 1990. the positive sera were further tested by plaque reduction neutralization test with the prototype hantaanvirus and the rat-associated hantaan like virus. isolation attempts were also performed from their tissues. antibody-positive rats were found in both slum areas, 32.7% in slum wat phai ton and 5.6% in slum klong toey. rattus norvegicus was the majo ...19921488708
susceptibility of laboratory and wild rodents to rattus or apodemus-type wistar rats (rattus norvegicus), apodemus agrarius, meriones unguiculatus, clethrionomys rufocanus, and apodemus argenteus were inoculated with rattus-type (sr-11, ki-262, and tb-314) or apodemus-type (hantaan 76-118) hantaviruses. production of serum antibody to the inoculated virus (iaha titres of 1:32 to 1:4 096) was obsersved in all rodent species 10 weeks after virus inoculation. rattus-type virus was detected in some organs of all the rodent species employed except of apodemus agrari ...19911683116
molecular characterization of the prospect hill virus m rna segment: a comparison with the m rna segments of other hantaviruses.complementary dna representing the genomic m rna segment of the prospect hill (ph) hantavirus was cloned and its nucleotide sequence determined. the ph virus m rna segment is 3707 nucleotides in length and has a long open reading frame in the viral complementary-sense rna with a coding capacity of 1142 amino acids. the predicted gene product of the ph virus m segment was compared with the corresponding gene products of hantaan virus strain 76-118 (hantaan), sapporo rat virus strain sr-11 (sr) an ...19911840609
identification and characterization of a hantavirus strain of unknown origin by nucleotide sequence analysis of the cdna derived from the viral s rna segment.the genetic characterization of a serologically hantaan-like virus but of unknown origin (termed dx) was carried out by molecular cloning and nucleotide sequencing of the corresponding cdna of the viral s rna segment. the s rna was found to be 1765 nucleotides long with 3' and 5' termini being complementary for 24 bases. the virus messenger-sense rna contains one major open reading frame (orf) encoding 428 amino acids or a 50 kd polypeptide. a comparison of the dx s rna segment to those of sappo ...19911905433
[virus-specific proteins and rna of the virus of hemorrhagic fever with renal syndrome].virus-specific proteins g1, g2, and n with molecular weights of 70, 55-57, and 50 kilodaltons, respectively, were detected by radioimmunodiffusion tests in vero e-6 cells infected with strains of virus of hemorrhagic fever with renal syndrome (hfrs) isolated in the european ussr from a patient with hfrs, a fatal human case of hfrs (the strains k-27 and p-360) and from a bank vole (strain cg-1820). the sera from human convalescents after hfrs in the european ussr and rat sera prepared with the cg ...19902114054
[study on geographic epidemiology of epidemic hemorrhagic fever (ehf) in china].the distribution, epidemic intensity and the focus type of ehf at county's level in china was studied by the method of discriptive, serological and geographic epidemiology. the ehf foci distributed in 1,257 counties within 25 provinces (cities, autonomous regions) by 1986, 633 of them were found before 1980 and the other 624 after it. the epidemic area have extended nearly twice in the last years. the extension of rattus type focus was most significant. the number of focus of rattus type increas ...19892567630
the prevalence of hantaan viruses among rattus norvegicus in tokyo in the 1960's.during the 1960's, 15 out of 89 rats (16.8%) captured in the port area and 6 out of 59 rats (10.2%) in the suburb of tokyo were seropositive against hantaan viruses.19892573224
serological survey of hantavirus infection in french polynesia. 19892575294
[cases of hemorrhagic fever renal syndrome in 3 counties of the guang xi zhuang tribe autonomous region for the first time with the recovery of hemorrhagic fever viral agents from infected rodents]. 19852859926
[main reservoirs of infection of the hemorrhagic fever renal syndrome in endemic areas of anhui province]. 19852862997
infection of laboratory workers with hantavirus acquired from immunocytomas propagated in laboratory rats.hantavirus has been isolated in cell culture from rat immunocytomas used and stored at a research laboratory in the u.k. where there was evidence of a laboratory-acquired infection leading to haemorrhagic fever with renal syndrome. both transplantation into lou/m/wsl rats and storage of passaged immunocytomas at -70 degrees c over a period of 8-10 years had not eliminated the virus. the isolates were identified as hantavirus by means of serum obtained from patients with hantavirus infection as w ...19862871112
clinical and epidemiological aspects of hemorrhagic fever with renal syndrome (hfrs) in greece.twenty-three cases of hemorrhagic fever with renal syndrome were serologically diagnosed in rural populations of greece between 1982 and 1985. the clinical picture of the disease was severe, (3 deaths recorded among 23 cases) and more closely resembled korean hemorrhagic fever than nephropathia epidemica. examination of patients' sera by indirect immunofluorescence (ifa) assays and plaque reduction neutralization (prnt) tests using hantaan 76-118, puumala, and seoul (urban rat-associated hantaan ...19872888688
haemorrhagic fever with renal syndrome: clinical, virological and epidemiological perspectives.haemorrhagic fever with renal syndrome (hfrs) is caused by a group of rna viruses within the family of bunyaviridae known as hantaviruses. the classical, severe form of hfrs is characterized by fever, headache, abdominal and lumbar pain, proteinuria, haemorrhagic phenomena, shock and renal failure. the disease is associated with the prototype hantaan virus and occurs in rural areas of korea and china with apodemus mice as reservoir hosts. a clinically less severe form of hfrs, which is caused by ...19872895603
haemorrhagic fever with renal syndrome: memorandum from a who meeting.haemorrhagic fever with renal syndrome (hfrs) is a public health problem throughout most of the european and asian land mass. although predominantly associated with rural areas, it is now being recognized as an urban problem in some countries, and also presents a particular hazard to laboratory staff who use rodents for biomedical research. in wild rodents (rats, mice and voles) the infection is asymptomatic. human infection with the hfrs agent(s) is sporadic, but under special circumstances epi ...19836134591
laboratory rat associated outbreak of haemorrhagic fever with renal syndrome due to hantaan-like virus in belgium.hantaan-like virus was identified as the cause of three cases of acute renal failure in staff handling laboratory rats on a belgian university campus. serological studies revealed infected rat strains at different sites on the campus and evidence of past and recent infections in 39 staff members and of infection in 50% of exposed staff. hantaan-reactive antibody was very rare in control populations. staff or rats at three other belgian institutions had no signs of infection. the origin of the vi ...19836140543
[prevalence of ectoparasites, endoparasites, bacteria and anti-hantaan virus antibodies in wild rats captured in harbor area of shimizu, japan]. 19846431033
experimental infection in newborn mice and rats by hemorrhagic fever with renal syndrome (hfrs) virus.newborn mice and rats were inoculated intracerebrally (ic) or intraperitoneally (ip) with hantaan virus (76-118 strain) or hfrs-related virus (b-1 strain). the mortality and the influence on the increase of body weight in newborn mice were higher in the groups infected with the 76-118 strain than in the groups infected with the b-1 strain, while the b-1 strain was more virulent in rats than the 76-118 strain. virus isolation from rats inoculated with either strain was attempted 7 and 11 weeks af ...19846442757
application of a recombinant baculovirus expressing hantavirus nucleocapsid protein as a diagnostic antigen in ifa test: cross reactivities among 3 serotypes of hantavirus which causes hemorrhagic fever with renal syndrome (hfrs).recombinant baculovirus-infected insect cells which expressed recombinant protein, analogous to the nucleocapsid protein (np) of seoul type hantavirus, strain sr-11 (rnp-sr-sf9) were applied to the indirect immunofluorescent antibody (ifa) test. the rnp-sr-sf9 reacted with anti np mab clones which recognized strain specific or hantavirus common epitopes in the ifa test. the recombinant antigen was detected by antibodies to 3 serotypes of hantaviruses (hantaan 76-118, sr-11, puumala). antibody ti ...19937906956
antigenic and molecular characterization of hantavirus isolates from china.hemorrhagic fever with renal syndrome (hfrs) is caused by certain viruses in the genus hantavirus, family bunyaviridae, and is a major public health problem in china. by using molecular and serological tests, we characterized 15 hantaviruses isolated either from patients with hfrs or from rodents captured in endemic areas of china. by cross plaque-reduction neutralization tests performed with rabbit immune sera, we identified two serologically distinct groups of viruses, comprised of those relat ...19947909976
a survey of hantavirus antibody in small-mammal populations in selected united states national parks.hantavirus activity in 39 national parks in the eastern and central united states was surveyed by testing 1,815 small mammals of 38 species for antibody reactive to sin nombre virus. antibody-positive rodents were found throughout the area sampled, and in most biotic communities. antibody was detected in 7% of 647 deer mice (peromyscus maniculatus), 2% of 590 white-footed mice (p. leucopus), 17% of 12 rice rats (oryzomys palustris), 3% of 31 cotton rats (sigmodon hispidus), and 33% of 18 western ...19989574803
isolation and genetic characterization of a hantavirus (bunyaviridae: hantavirus) from a rodent, oligoryzomys microtis (muridae), collected in northeastern peru.this paper describes the isolation and partial genetic characterization of a hantavirus from a pygmy rice rat, oligoryzomys microtis, collected within the urban area of iquitos, loreto department, peru. the virus, designated htn-007, exhibited the highest degree of genetic similarity to rio mamore virus, which was originally described from the same rodent species in eastern bolivia. comparison of small and medium segment nucleotide sequence data from htn-007 and rio mamore virus revealed 87% and ...199910432063
low ph-induced cytopathic effect--a survey of seven hantavirus strains.hantaviruses do not produce cytopathic effects (cpe) in cell culture. however, a syncytial cpe can be induced in 7-day cultures of hantavirus growing in vero e6 cells by reduction of the ph to approximately 6.2 using a hepes based buffer. the appearance of this acid induced cpe was examined for seven different hantavirus strains. the differences noted were striking and reflected the taxonomic differences between hantaviruses. at 10-100 tcid50% the size of syncytial foci was very large for seoul ...199910488778
hantavirus (bunyaviridae) infections in rodents from orange and san diego counties, california. 199910674658
experimental infection of the sigmodon alstoni cotton rat with caño delgadito virus, a south american hantavirus.forty-eight sigmodon alstoni (alston's cotton rat) were inoculated with caño delgadito (cdg) virus to extend our knowledge and understanding of the natural host relationships of the hantaviruses indigenous to the americas. infectious cdg virus was recovered from oropharyngeal secretions, urine, or solid tissues of nine of 12 animals killed on day 9 post-inoculation (pi), 14 of 24 animals killed on day 18 or 27 pi, and none of 12 animals killed on day 54 pi. in addition, virus-specific rna was de ...200212363052
hantaviruses: a tale of two hemispheres.infectious febrile nephropathies, initially described as epidemics during military campaigns in europe and asia at the turn of the 20(th) century, were eventually all unified under the rubric of hemorrhagic fever with renal syndrome (hfrs) and ascribed to infection from rodent-borne hantaviruses. at the end of the century, hantavirus pulmonary syndrome (hps) was described throughout the americas as a consequence of infection with the new world hantaviruses. the epidemiology and clinical manifest ...200312682619
[the epidemic characteristics and preventive measures of hemorrhagic fever with syndromes in china].to summarize and analyze the epidemic situation of hantaviruses including geographic distribution, types and prevalent intensity of epidemic areas of hantavirus for the last 30 years in china, and to discuss relative preventive measures.200415231118
norway rat population in baltimore, maryland, 2004.norway rats are reservoirs for several zoonotic agents, including hantaviruses, and are implicated in the transmission of pathogens to humans in urban environments. the rat population of baltimore, maryland was estimated from surveys in 1949 and again in 1952, but has not been evaluated for more than 50 years. previously identified sociodemographic risk factors for rat infestation, including median income, human density, and percentage of rental properties, were used to categorize census block g ...200516187901
[first serological evidence of hantavirus infection in rodents in colombia].determining hantavirus infection prevalence in rodents in the c6rdoba department, colombia.200616925117
temporal and spatial host abundance and prevalence of andes hantavirus in southern argentina.andes virus (and) is a hantavirus hosted by the sigmodontine rodent oligoryzomys longicaudatus in southern argentina, where it is responsible for most cases of hantavirus pulmonary syndrome (hps). our study provides data about the spatial variation in abundance of the rodent host of and hantavirus. we report results of a longitudinal study performed in a locality of the andean region of chubut province. from november 2003 (spring) to july 2006 (winter), o. longicaudatus was the most common speci ...201020645121
evaluation of focus reduction neutralization test with peroxidase-antiperoxidase staining technique for hemorrhagic fever with renal syndrome virus.titers of hemorrhagic fever with renal syndrome virus were estimated by counting foci stained with peroxidase-antiperoxidase or by using immunofluorescence methods. foci stained with pap were clearer and easier to count. the peroxidase-antiperoxidase method showed a linear relationship between virus concentration and the number of foci. a focus-reduction neutralizing antibody test was performed with immune rat sera and sera from patients. again, the peroxidase-antiperoxidase method proved to be ...19846440908
serotypic classification of hantaviruses by indirect immunofluorescent antibody and plaque reduction neutralization tests.antisera prepared against 16 strains of hantaviruses isolated from patients with hemorrhagic fever with renal syndrome (hfrs) or from rodents captured in hfrs-endemic and nonendemic regions were titrated against hantaan virus strain 76-118, puumala virus strain sotkamo, and prospect hill virus strain prospect hill-i by using the indirect immunofluorescent antibody and plaque reduction neutralization tests. isolates fell into one of four distinct groups or serotypes. serotype 1 included apodemus- ...19852866197
serological studies of haemorrhagic fever with renal syndrome (hfrs) in poland. preliminary report.serological studies of hantaan virus infections among--blood donors (control group)--patients: chronic dialysed, leptospirosis suspected, hospitalised for various diseases,--risk group population: farmers, laboratory staff (contact with laboratory animals), as well as laboratory and wild animals (rodents) were carried out. surveys were done by the indirect immunofluorescent antibody (ifa) procedure with 5 hantaan virus strains as antigens.19892476002
virion proteins of viruses causing hemorrhagic fever with renal syndrome; monoclonal antibody analysis.sds-polyacrylamide gel electrophoresis and western blotting analysis by using 9 clones of monoclonal antibodies specific for hemorrhagic fever with renal syndrome (hfrs) viruses were carried out on the virion proteins of 17 strains of hfrs viruses isolated from different areas in asia and different hosts such as apodemus agrarius, rattus norvegicus, domestic cat and hfrs patients. polypeptide with apparent molecular weight about 50 kitodalton (kd) could be detected in all 17 strains of hfrs viru ...19892474668
[experiment research on moxibustion treating infected ehfv rat].in this experiment, we established a rat model of ehfv infected by abdominal cavity, and observed the effect of moxibustion on the changes of antigens, antibodies, neural transmitters in blood and tissue on 60 weanling wistar rats with ehf. the rats were divided into three groups: normal control group (n. group), ehf control group (c. group) and ehf with moxibustion group (m. group) at random. moxibustion was given at "shenshu" for 30 minutes daily, 7 days. the 14th day after infection of ehfv, ...19901980432
isolation of a strain of hantaan virus from peritoneal exudate cells of a patient with hemorrhagic fever with renal syndrome.a new strain of hantaan virus (htnv), gh716, was isolated from the peritoneal exudate cells (pec) of a patient with severe hemorrhagic fever with renal syndrome (hfrs). the isolate was propagated in vero e6 cells. at each passage the virus-infected cells were examined for htnv with immunofluorescence technique using monoclonal antibodies (mcab) 25-1 and 84-1 against htnv. from passage 5 on, fluorescent intensity tended to stabilize at (+++) and infectivity titer reached 10(5) tcid50/ml. using 14 ...19901976488
[effect of moxibustion on the immune and anti-virus functions of the rat infected with epidemic hemorrhagic fever virus]. 19921364108
[study on the serological typing of patients with epidemic hemorrhagic fever (ehf) and host animals in junan county, shangdong province].using hemagglutination inhibition test (hi), we confirmed that junan county is an endemic area of both ehf of r. norvegicus and a. agrarius, which indicated that the main host animals were r. norvegicus and a. agrarius in the area. remarkable difference was shown between the infectious rates of pigs from the endemic area and that of pigs from the nonendemic area, which suggested that pigs could play certain roles in the transmission of ehf. we also proved that the main transmission mode of ehf v ...19921356628
application of truncated nucleocapsid protein (n) for serotyping elisa of murinae-associated hantavirus infection in rats.truncated recombinant nucleocapsid proteins (trns) that lack n-terminally located cross-reactive epitopes of four murinae rodent-associated hantaviruses, seoul virus (seov), thailand virus, hantaan virus (htnv) and dobrava-berglade virus, were produced by using a baculovirus expression system. elisa with the trns as antigens enabled serotyping of immune sera from rats experimentally inoculated with the corresponding hantaviruses as cut-off od values of 60% to those to whole n of htnv. the trn ba ...201121937858
corticosteroids modulate seoul virus infection, regulatory t-cell responses and matrix metalloprotease 9 expression in male, but not female, norway rats.human hantaviral disease is mediated by excessive proinflammatory and cd8+ t-cell responses, which can be alleviated by administration of corticosteroids. in contrast with humans, male rats that are infected with their species-specific hantavirus, seoul virus (seov), have reduced proinflammatory and elevated regulatory t-cell responses in tissues where virus persists. to determine the effects of glucocorticoids on seov persistence and immune responses during infection, male and female norway rat ...200818931068
seoul virus enhances regulatory and reduces proinflammatory responses in male norway rats.zoonotic pathogens, including hantaviruses, are maintained in the environment by causing persistent infection in the absence of disease in their reservoir hosts. spillover of hantaviruses to humans can cause severe disease that is mediated by excessive proinflammatory responses. the mechanisms mediating hantaviral persistence in rodent reservoirs remain largely unknown. male norway rats were inoculated with their species-specific hantavirus, seoul virus (seov), and viral rna, cytokine, and chemo ...200818461618
first evidence of fatal hantavirus nephropathy in india, mimicking leptospirosis. 200616361279
persistent seoul virus infection in lewis rats.mechanistic studies of hantavirus persistence in rodent reservoirs have been limited by the lack of a versatile animal model. this report describes findings from experimental infection of inbred lewis rats with seoul virus strain 80-39. rats inoculated with virus intraperitoneally at 6 days of age became persistently infected without clinical signs. tissues from seoul virus-inoculated 6-day-old rats were assessed at 6, 9, and 12 weeks post-inoculation for viral rna by rt-pcr and in situ hybridiz ...200415221534
[discovery of a new subtype of seo virus].the m and s segments of cou3, a hantavirus strain isolated from rattus norvegieus were partially reverse transcripted, pcr amplified and sequenced with abi 373 dna sequencer to observe the differences from other seo type viruses.200011471015
[hantavirus infection: a "new" exotic zoonosis among us].hantavirus disease is a recently recognized zoonosis. the main vectors are infected but healthy wild rodents or laboratory rats. transmission to man occurs via inhalation of aerosolised excretions. kidneys or lungs are the target organs during the disease, but a systemic affection with multi-organ involvement is possible, and can be fatal. to date, at least 16 different serotypes of hantavirus have been characterized, 8 of them being of clinical importance. each serotype has its own rodent vecto ...19969156672
wild brown rats (rattus norvegicus) as a zoonotic risk on farms in england and wales.fear of wild rats as carriers of disease is embedded within our culture. surprisingly little is actually known about parasites in wild rat populations. in recent studies aimed to rectify this omission, farms in england and wales were surveyed to identify parasite species present in the rat populations. rats were found to be infected with numerous zoonotic parasites, including cryptosporidium, pasteurella [correction of pasturella], listeria, yersinia, coxiella, and hantavirus. these findings sug ...19968820193
[serotyping of patients with epidemic hemorrhagic fever (ehf) and serotyping and antigen typing of main hosts of ehf in shandong province].serotyping with hemagglutination-inhibition test (hi) on 458 patients with ehf showed that 419 (91.48%) of them belonged to the house-rat type, 33 (7.21%) belonged to the wild-rat type, and 6 (1.31%) were undetermined. the majority (80-100%) of the patients in various regions belonged to the house-rat type. the type of the virus antigen and the type of the antibody of rats were in conformity with the species of rats. these indicate that the epidemic areas of ehf in shandong province are mixed-ty ...19938099309
serological evidence of hantavirus disease in northern ireland.since, to our knowledge, no clinically documented cases of haemorrhagic fever with renal syndrome (hfrs) have been reported in northern ireland, a sero-epidemiological study was carried out to assess the degree of hantavirus immunity in a group of 627 northern irish patients presenting with symptoms suggestive of hfrs and 100 healthy controls. ifa screening for igg hantavirus specific antibodies was carried out with a panel of up to 9 different hantaviral antigens. igm screening was performed us ...19947916033
protection against hantavirus infection by dam's immunity transferred vertically to neonates.antibodies to hantavirus, seoul type b-1 strain, vertically transferred to rat neonates prevented lethal as well as persistent infection. when relatively high titer viruses were inoculated into neonates, the mother's antibodies protected all the neonates from lethal virus infection. however, the antibodies could not protect all of the neonates from persistent infection but only half of them underwent persistent infection. the other half was completely cured but also became persistently infected ...19957646341
infectivity titration of hemorrhagic fever with renal syndrome virus: use of immune adherence hemagglutination for detection of virus growth.serial dilutions of hemorrhagic fever with renal syndrome viruses were inoculated into vero-e6 cells in microplates. after 2 weeks of incubation, infected cells were disrupted by freezing and thawing, and virus antigens were detected by immune adherence hemagglutination. the infectivity titers of the virus as determined by this method were in close agreement with those obtained by the immunofluorescent antigen endpoint method. then, a neutralization method was established. japanese hemorrhagic f ...19846436305
isolation of hemorrhagic fever with renal syndrome (hfrs) virus from a tumor specimen in a rat.a strain of hemorrhagic fever with renal syndrome (hfrs) virus was isolated in a cell culture from a tumor specimen in a rat kept in a medical institution in which there was a case of hfrs. positive immunofluorescent reaction with sera from hfrs patients was recognized at the second passage and the number of cells containing antigen increased in the third passage. this virus, named b-1 strain, was identified as the hfrs virus by immunofluorescent tests with sera from patients.19836146307
enzyme-linked immunosorbent assay for detection of antibody against hemorrhagic fever with renal syndrome virus in sera from experimentally infected rats. 19872892958
[the role of hantaan virus serotypes in the etiology of hemorrhagic fever with renal syndrome in the far east of the ussr].the employment of two serological tests (indirect fluorescence antibody technique and neutralization test) demonstrated the leading role of hantaan virus serotype 1 strains isolated from field mice in the pattern of the incidence of hemorrhagic fever with renal syndrome (hfrs). proofs of the etiological importance of strains of serotypes 3 and 5 occurring in brown rats and cl. rufocanus were obtained. no data on any association of human hfrs cases with strains of serotype 4 isolated from reed vo ...19901982032
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