| hsv-1 vector-mediated gene transfer of the human nerve growth factor receptor p75hngfr defines high-affinity ngf binding. | a series of recombinant herpes simplex virus (hsv-1) vectors have been constructed that encode either the full-length cdna of the human p75 ngf receptor (p75hngfr) or truncated forms of the receptor. infection of cultured fibroblast cells with viral stocks results in abundant expression of all three cdnas, as detected by affinity cross-linking, immunoblot analysis, and equilibrium binding. furthermore, viral infection of primary neuronal cultures gives easily detectable p75 expression by immunof ... | 1993 | 8382740 |
| the herpes simplex virus type 1 us11 gene product is a phosphorylated protein found to be non-specifically associated with both ribosomal subunits. | microsequencing of a cyanogen bromide peptide obtained from a basic phosphoprotein co-sedimenting with purified ribosomes extracted from herpes simplex virus type 1-infected human epidermoid carcinoma 2 cells identified this protein as a product of the true late us11 gene. an antibody was raised against a recombinant fusion protein expressed in escherichia coli from a plasmid carrying 75% of the us11 coding sequence including the carboxy terminus. this antibody was used to probe western blots ca ... | 1993 | 8383175 |
| the pseudorabies virus host-shutoff homolog gene: nucleotide sequence and comparison with alphaherpesvirus protein counterparts. | the virion host shutoff function (vhs) of many herpesviruses is required for inhibition of cellular gene expression in infected cells. this function corresponds to the ul41 open reading frame of herpes simplex virus type 1 (hsv-1) and homolog sequences have been found in other alphaherpesvirus, like hsv-2, varicella-zoster virus (vzv) and equine herpesvirus type 1 (ehv-1). in this work, we have cloned and sequenced a pseudorabies virus (prv) gene which is homologous to the vhs genes of the other ... | 1993 | 8384744 |
| purification and properties of the herpes simplex virus type 1 ul8 protein. | a rapid and simple two-step scheme for the purification of herpes simplex virus type 1 ul8 protein from insect cells infected with a recombinant baculovirus has been developed. the scheme involves deae-sepharose and phenyl-sepharose chromatography and yields approximately 1.5 mg of protein from 2.4 x 10(8) infected cells. the protein remains intact during purification as judged by its reactivity with amino and carboxy termini-specific antisera. gel filtration chromatography showed that the prote ... | 1993 | 8385691 |
| the ggtca palindrome and cognate cellular factors in trans-regulation of human immunodeficiency virus long terminal repeat by herpes simplex virus. | molecular interactions between herpes simplex virus type 1 (hsv-1) and human immunodeficiency virus (hiv) were investigated in the promonocytic cell line u937. hsv-1-mediated activation was observed in transient expression assays with hybrid constructions containing the hiv long terminal repeat (ltr)-directed chloramphenicol acetyltransferase gene. comparison of constructions that differ in the ggtca palindrome located within the negative regulatory region of the ltr revealed four- to eightfold ... | 1993 | 8385697 |
| induction of bilateral retinal necrosis in mice by unilateral intracameral inoculation of a glycoprotein-c deficient clinical isolate of herpes simplex virus type 1. | herpes simplex virus can cause acute retinal necrosis, a blinding retinal disease in man. a unilateral intracameral inoculation of herpes simplex virus type 1 (hsv-1) in mice induces retinal necrosis primarily in the contralateral eye and provides an experimental model for the disease. previous studies suggested that a major envelope glycoprotein of hsv-1, glycoprotein c (gc), is required for retinal necrosis. we studied hsv-1 strain tn-1, a gc-deficient clinical isolated from a lesion of herpet ... | 1993 | 8385909 |
| varicella-zoster virus open reading frame 10 protein, the herpes simplex virus vp16 homolog, transactivates herpesvirus immediate-early gene promoters. | the varicella-zoster virus (vzv) open reading frame 10 (orf10) protein is the homolog of the herpes simplex virus type 1 (hsv-1) protein vp16. these are two virion tegument proteins that have extensive amino acid sequence identity in their amino-terminal and middle domains. orf10, however, lacks the acidic carboxy terminus which is critical for transactivation by vp16. earlier studies showed that vzv orf10 does not form a tertiary complex with the taatgarat regulatory element (where r is a purin ... | 1993 | 8386275 |
| altering central nervous system physiology with a defective herpes simplex virus vector expressing the glucose transporter gene. | because of their postmitotic nature, neurons are difficult subjects for gene transfer. to circumvent this, we have used a defective herpes simplex virus vector to overexpress the rat brain glucose transporter (gt) gene under the control of the human cytomegalovirus ie1 promoter. this vector, designated vie1gt, was propagated using a herpes simplex virus type 1 temperature-sensitive mutant, ts756. gt expressed from vie1gt was readily immunoprecipitated from membrane fractions of vie1gt-infected v ... | 1993 | 8386379 |
| in vitro antiviral activity of polyoxomolybdates. mechanism of inhibitory effect of pm-104 (nh4)12h2(eu4(moo4)(h2o)16(mo7o24)4).13h2o on human immunodeficiency virus type 1. | a screening for inhibitors of human immunodeficiency virus type 1 (hiv-1) among various types of isopolyoxomolybdates and heteropolyoxomolybdates was carried out by using an in vitro assay system measuring the cytopathogenicity of hiv-1 in cd4+ human mt-4 cells. a novel heteropolyoxomolybdate named pm-104 with the chemical formula (nh4)12h2(eu4(moo4)(h2o)16(mo7o24)4).13h2o was found to be associated with potent anti-hiv-1 activity. pm-104 interferes with virus infection at a very early step such ... | 1993 | 8387260 |
| intranasal immunization against herpes simplex virus infection by using a recombinant glycoprotein d fused with immunomodulating proteins, the b subunit of escherichia coli heat-labile enterotoxin and interleukin-2. | to establish a novel strategy of mucosal immunization against herpes simplex virus type 1 (hsv-1) infection, we studied the immune responses elicited by intranasal immunization with several forms of a recombinant glycoprotein d (gd) of hsv-1. a truncated gd (t-gd) co-administered with heat-labile enterotoxin b subunit (ltb) from escherichia coli induced both a mucosal immune response involving secretion of anti-gd iga and serum igg production. the levels of these responses are comparable to thos ... | 1993 | 8388365 |
| direct identification of herpes simplex virus type-1 in human corneal tissue. methodology and application of in situ dna hybridization technique. | the definitive diagnosis and determination of recurrence of herpes simplex keratitis are still difficult in clinical ophthalmology. at present, isolation of virus by tissue culture is perhaps the best method for establishing a specific aetiological diagnosis of viral infection. but due to its complicated and time-consuming procedures, the application of tissue culture for virus isolation in clinical work is still limited. in situ dna hybridization is a specific and quick technique for directly d ... | 1993 | 8389272 |
| age distribution of latent herpes simplex virus 1 and varicella-zoster virus genome in human nervous tissue. | latency in nervous tissue caused by herpes simplex virus 1 (hsv-1) and by varicella-zoster virus (vzv) is an intriguing feature of herpes-virus' neurotropism. hsv-1 and vzv latency are the causes of ophthalmic zoster and recurrent hsv infections in the distributions of the trigeminal branches. hsv-1 neuronal latency may play a role in the etiopathogenesis of hsv encephalitis. we attempted to determine the prevalence and age distribution of vzv and hsv latency. we applied nested polymerase chain ... | 1993 | 8389816 |
| varicella-zoster virus (vzv) open reading frame 61 protein transactivates vzv gene promoters and enhances the infectivity of vzv dna. | the varicella-zoster virus (vzv) open reading frame 61 (orf61) protein is the homolog of herpes simplex virus type 1 (hsv-1) icp0. both genes are located in similar parts of the genome, their predicted products share a cysteine-rich motif, and cell lines expressing vzv orf61 are able to complement an hsv-1 icp0 deletion mutant (h. moriuchi, m. moriuchi, h. a. smith, s. e. straus, and j. i. cohen, j. virol. 66:7303-7308, 1992). in transient expression assays, hsv-1 icp0 is a transactivator alone ... | 1993 | 8389928 |
| antineoplastic and antiviral activities of some cyclolignans. | nineteen cyclolignans of varied structures, most of them isolated from juniperus sabina leaves, were evaluated for their antineoplastic and antiviral activities. they were subjected to screening against p-388 murine leukemia, a-549 human lung carcinoma, and ht-29 colon carcinoma, while the antiviral assays were performed on herpes simplex virus type 1 infecting fibroblasts of monkey kidney (hsv-1/cv-1) and on vesicular stomatitis virus infecting fibroblasts of hamster kidney (vsv/bhk). a number ... | 1993 | 8391145 |
| identification of the gene product encoded by orf ul56 of the human cytomegalovirus genome. | experiments were undertaken to identify the product of open reading frame (orf) ul56 of human cytomegalovirus (hcmv), the putative homolog of infected cell protein (icp) 18.5 of herpes simplex virus type 1 (hsv-1) that is thought to be involved in viral nucleocapsid maturation. northern blotting using a unique fragment of orf ul56 revealed a specific transcript of about 3.0 kb in hcmv-infected fibroblasts early and late postinfection (p.i.). two overlapping fragments of ul56 were subcloned for p ... | 1993 | 8395117 |
| inhibition of herpes simplex virus type 1 dna replication by mutant forms of the origin-binding protein. | the herpes simplex virus type 1 (hsv-1) origin-binding protein (obp) is a sequence-specific dna binding protein encoded by gene ul9 which interacts with the viral origins of dna replication and also exhibits dna helicase activity. sequence-specific dna binding activity has previously been shown to reside within the c-terminal 317 amino acids, and when expressed alone, this domain exerts a dominant inhibitory effect on hsv-1 dna synthesis. we have tested several ul9 gene mutants for ability to su ... | 1993 | 8396795 |
| [double meningoencephalitis with herpes simplex virus and rubella virus]. | a 47-year-old man suffered from a headache, fever and memory disturbance. he was admitted to morimoto hospital. neurological examination revealed disturbance of memory of recent events. cranial nerves were normal. muscle strength of the extremities was normal, except for barré's sign of the right upper extremity. deep tendon reflexes were exaggerated bilaterally, and extensor plantar response of the left side was elicited. sensory examination showed no abnormality. cerebellar sign was not recogn ... | 1993 | 8403685 |
| herpes simplex virus glycoprotein c: molecular mimicry of complement regulatory proteins by a viral protein. | herpes simplex virus (hsv) encodes a protein, glycoprotein c (gc), which binds to the third complement component, the central mediator of complement activation. in this study the structural and functional relationships of gc from hsv type 1 (hsv-1) and known human complement regulatory proteins factor h, properdin, factor b, complement receptor 1 (cr1) and 2 (cr2) were investigated. the interaction of gc with c3b was studied using purified complement components, synthetic peptides, antisera agai ... | 1993 | 8406590 |
| an evaluation of oral ulcers in patients with aids and aids-related complex. | patients with hiv infection can have recurrent and persistent oral ulcers, not attributable to known infectious agents. | 1993 | 8408791 |
| [from herpes to zona]. | | 1993 | 8410632 |
| antiviral activity of c-alkylated purine nucleosides obtained by cross-coupling with tetraalkyltin reagents. | 2-, 6-, and 8-alkylated (methyl, ethyl, and vinyl) adenosine analogues were synthesized by a palladium-catalyzed cross-coupling of a tetraalkyltin with the halogenated purine nucleosides. the synthesis of the 8-substituted analogues was accomplished using a transient protection procedure. the 6-alkylated-9-beta-d-ribofuranosylpurines as well as 2-ethyladenosine were cytotoxic at relatively low concentrations (0.8-10 micrograms/ml). 8-methyladenosine was a potent and selective inhibitor of vaccin ... | 1993 | 8411010 |
| inhibition of herpes simplex virus type 1 ribonucleotide reductase by substituted tetrapeptide derivatives. | it is known that peptides corresponding to the c-terminus of the small subunit of herpes simplex virus type 1 and 2 ribonucleotide reductase can inhibit enzymatic activity by preventing the association of the enzyme's two subunits. in a quest for smaller, more potent inhibitors, we have conducted a structure activity investigation based on the pentapeptide h-val-val-asn-asp-leu-oh. potency increases of up to 4000 times (ic50 0.18 microm) have been achieved in an enzymatic assay by a combination ... | 1993 | 8411018 |
| differential antiherpesvirus and antiretrovirus effects of the (s) and (r) enantiomers of acyclic nucleoside phosphonates: potent and selective in vitro and in vivo antiretrovirus activities of (r)-9-(2-phosphonomethoxypropyl)-2,6-diaminopurine. | the (s)- and (r)-enantiomers of acyclic purine nucleoside phosphonate analogs (i.e., 3-hydroxy-2-phosphonomethoxypropyl [hpmp] derivatives, 3-fluoro-2-phosphonomethoxypropyl [fpmp] derivatives, and 2-phosphonomethoxypropyl [pmp] derivatives of adenine [a], 2-aminopurine, 2,6-diaminopurine [dap], and guanine [g]) have been synthesized and evaluated for antiviral activity. as a rule, the hpmp derivatives proved effective against dna viruses but not rna viruses or retroviruses. in particular, (s)-h ... | 1993 | 8452366 |
| novel acyclonucleotides: synthesis and antiviral activity of alkenylphosphonic acid derivatives of purines and a pyrimidine. | a series of phosphonoalkenyl and (phosphonoalkenyl)oxy derivatives of purines and a pyrimidine were synthesized. these compounds are the first reported acyclonucleotides which incorporate the alpha,beta-unsaturated phosphonic acid moiety as the phosphate mimic and include compounds in which the acyclic substituent is attached to n-9 of a purine or n-1 of a pyrimidine by either a nitrogen-carbon or a nitrogen-oxygen bond. the phosphonoalkenyl-substituted compounds 7a-c, 8a-c, 9, 10, and 12 were p ... | 1993 | 8496903 |
| inflammatory infiltration of the trigeminal ganglion after herpes simplex virus type 1 corneal infection. | following herpes simplex virus type 1 (hsv-1) infection of the cornea, the virus is transmitted to the trigeminal ganglion, where a brief period of virus replication is followed by establishment of a latent infection in neurons. a possible role of the immune system in regulating virus replication and maintaining latency in the sensory neurons has been suggested. we have investigated the phenotype and cytokine pattern of cells that infiltrate the a/j mouse trigeminal ganglion at various times aft ... | 1996 | 8523535 |
| cloning of the cdnas for the small subunits of bovine and human dna polymerase delta and chromosomal location of the human gene (pold2). | cdnas encoding the small subunit of bovine and human dna polymerase delta have been cloned and sequenced. the predicted polypeptides, 50,885 and 51,289 daltons, respectively, are 94% identical, similar to the catalytic subunits. the high degree of conservation of the polypeptides suggests an essential function for the small subunit in the heterodimeric core enzyme. although the catalytic subunit of dna polymerase delta shares significant homology with those of the herpes virus family of dna poly ... | 1995 | 8530069 |
| seroepidemiology of herpes virus infections among dental personnel. | to examine the possible occupational hazard of infection with human herpes viruses among dental personnel. | 1995 | 8530723 |
| liposome-encapsulated (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine for long-acting therapy of viral retinitis. | the effect of liposome-encapsulated (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (hpmpc; cidofovir) was evaluated as prophylaxis in a rabbit model of experimentally induced retinitis caused by preretinal inoculation of herpes simplex virus type 1 (hsv-1). cidofovir (100 micrograms) in liposomes (0.1 ml) was injected intravitreally 10-120 days before retinal inoculation with hsv-1. twenty-two of 26 eyes pretreated with liposome-encapsulated cidofovir 10-60 days before hsv-1 inoculation wer ... | 1996 | 8537656 |
| herpes simplex virus protein targets for cd4 and cd8 lymphocyte cytotoxicity in cultured epidermal keratinocytes treated with interferon-gamma. | in early recurrent herpetic lesions, cd4 t lymphocytes are the predominant infiltrating cells, and keratinocytes expressing major histocompatibility complex (mhc) class ii antigens, induced by interferon-gamma (ifn-gamma), are the major site of herpes simplex virus (hsv) replication. ifn-gamma pretreatment of human keratinocytes in vitro reduced mhc class i antigen down-regulation by hsv-1 infection and induced expression of hla-dr that was unaltered by subsequent hsv-1 infection. incubation of ... | 1996 | 8537685 |
| post-transcriptional transactivation of human retroviral envelope glycoprotein expression by herpes simplex virus us11 protein. | herpes simplex virus type 1 (hsv-1) us11 protein, a true late gene product packaged within the virion, is delivered into cells after infection, exhibits a nucleocytoplasmic localization at early times, and later accumulates in the nucleoli. this rna-binding basic phosphoprotein, capable of oligomerization, is supposed to be involved in post-transcriptional regulation of gene expression after hsv-1 infection. expression of human t-cell leukaemia/lymphoma virus type-i (htlv-i) and of human immunod ... | 1996 | 8538795 |
| synthesis, antiviral activity and enzymatic phosphorylation of 9-phosphonopentenyl derivatives of guanine. | (e)-9-(5-phosphonopent-4-enyl)guanine and (e)-9-[3-(hydroxymethyl)-5- phosphonopent-4-enyl]guanine which bear a vinyl phosphonate moiety as a mimic of the phosphate group were synthesized. their activities against human immunodeficiency virus type-1 (hiv-1), herpes simplex virus type-1 (hsv-1) and human cytomegalovirus (hcmv) were evaluated in vitro in parallel with those of 9-(5-phosphonopentyl)guanine and 9-(5,5-difluoro-5- phosphonopentyl)guanine. both vinyl phosphonates exhibited anti-hiv-1 ... | 1995 | 8540751 |
| the ribonucleotide reductase inhibitor (e)-2'-fluoromethylene-2'-deoxycytidine (mdl 101,731): a potential topical therapy for herpes simplex virus infection. | the ribonucleotide reductase inhibitor mdl 101,731 was examined for antiviral activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in vitro and in combination with acyclovir in the murine zosteriform model of hsv-1 infection. the in vitro antiviral activity (ic50) for both serotypes of hsv was similar and in the range 23-98 nm for vero cells. comparable activities were obtained against acyclovir-resistant viruses. in the zosteriform model, topical combination therapy of mdl 10 ... | 1995 | 8540753 |
| herpes simplex virus-1 latency and its implications for gene therapy of the nervous system. | | 1994 | 8542397 |
| stimulation of neurite growth rat adrenal chromaffin cells infected with a defective herpes simplex virus carrying an ngf minigene. | | 1994 | 8542416 |
| varicella-zoster virus gene regulation. | the varicella-zoster virus genome contains 71 open reading frames (orfs), five of which (orf62, orf4, orf63, orf61, and orf10) encode regulatory proteins. orf62 codes for the major immediate early protein of the virus exhibiting dna-binding and regulatory functions. this protein, localized in the cell nucleus, is a functional homologue to icp4 of herpes simplex virus type 1 (hsv-1). it trans-activates several varicella-zoster virus promoters of the various gene classes and autoregulates its own ... | 1995 | 8545012 |
| transcriptional mapping of varicella-zoster virus regulatory proteins. | varicella-zoster virus (vzv) expresses four proteins that influence viral transcriptional events and that also are homologous to herpes simplex virus type 1 (hsv-1) immediate-early proteins. however, their transcription and the mechanisms by which it is regulated are not yet resolved. to identify the promoter regions, a precise knowledge of the initiation and termination of the encoded rnas is first required. in this report, we summarize the complete and precise mapping of the rna transcripts of ... | 1995 | 8545015 |
| molecular evolution of infectious laryngotracheitis virus (iltv; gallid herpesvirus 1): an ancient example of the alphaherpesviridae? | an analysis of two essential genes of infectious laryngotracheitis virus (iltv), glycoprotein d (gd) and the immediate early gene, herpes simplex virus homologue icp27, was performed with the equivalent gene homologues from several alphaherpesviruses. amino acid (aa) sequence analysis revealed that these iltv genes shared limited homology to other alphaherpesvirus equivalents and were distinct from the two other avian herpesviruses, marek's disease virus (mdv) and herpesvirus of turkeys (hvt). s ... | 1995 | 8545960 |
| acyclovir-resistant chronic cutaneous herpes simplex in wiskott-aldrich syndrome. | a 28-year-old man with wiskott-aldrich syndrome presented with ulcerative-proliferative lesions on his face from which herpes simplex type 1 (hsv-1) was isolated. he was initially treated with 10 mg/kg of acyclovir (zovirax) intravenously every 8 h, but his skin lesions worsened. clinical resistance to acyclovir was suspected, and therapy with this drug was intensified. the dosage of acyclovir was increased to 45 mg/kg, administered by continuous infusion, and the lesions subsequently resolved. ... | 1995 | 8547009 |
| rolling circle dna replication by extracts of herpes simplex virus type 1-infected human cells. | whole-cell extracts of herpes simplex virus type 1-infected human cells (293 cells) can promote the rolling circle replication of circular duplex dna molecules. the products of the reaction are longer than monomer unit length and are the result of semiconservative dna replication by the following criteria: (i) resistance to dpni and susceptibility to mboi restriction enzymes, (ii) shift in density on a cscl gradient of the products synthesized in the presence of bromo-dutp to a position on the g ... | 1996 | 8551573 |
| resistance of herpes simplex virus type 1 to peptidomimetic ribonucleotide reductase inhibitors: selection and characterization of mutant isolates. | herpes simplex virus (hsv) encodes its own ribonucleotide reductase (rr), which provides the high levels of deoxynucleoside triphosphates required for viral dna replication in infected cells. hsv rr is composed of two distinct subunits, r1 and r2, whose association is required for enzymatic activity. peptidomimetic inhibitors that mimic the c-terminal amino acids of r2 inhibit hsv rr by preventing the association of r1 and r2. these compounds are candidate antiviral therapeutic agents. here we d ... | 1996 | 8551616 |
| a screening system for antiviral compounds against herpes simplex virus type 1 using the mtt method with l929 cells. | we developed the improved mtt assay system for in vitro evaluation of anti-hsv-1 agents using l929 cells derived from mouse connective tissue. this assay system provides results in 4 days using a 96-well microplate. the ec50 values of several anti-hsv agents (acv, bvarau, and others) were found to be similar to those obtained by the plaque reduction method using mrc-5 cells. the present mtt assay is rapid, accurate, and may be useful as an automatic screening system for evaluation of anti-hsv-1 ... | 1995 | 8553354 |
| herpes simplex virus type 1-mediated up-regulation of il-12 (p40) mrna expression. implications in immunopathogenesis and protection. | in this report we investigated whether induction of il-12 occurs in response to herpes simplex virus (hsv) infection of the mouse eye, which may serve to regulate the nature of the subsequent immune response. the data show early induction and continued maintenance of il-12 (p40) mrna in the cornea and draining lymph node upon ocular infection with hsv. using a very sensitive radioimmunoassay technique, il-12 (p40) protein also was detected in the cornea upon ocular hsv infection. unfractionated ... | 1996 | 8557986 |
| inactivation of viruses by chemically and photochemically generated singlet molecular oxygen. | inactivation of viruses in blood plasma can be achieved by photodynamic procedures using methylene blue (mb) or other photoactive dyes. singlet molecular oxygen (1o2) probably contributes to the virucidal effects of photosensitization. we report the inactivation of herpes simplex virus type 1 (hsv-1) and suid herpes virus type 1 (shv-1) by chemically generated singlet oxygen, produced by thermal decomposition of the endoperoxide of 3,3'-(1,4-naphthylidene)dipropionate (ndpo2). we demonstrate tha ... | 1995 | 8558363 |
| transcription factors interacting with herpes simplex virus alpha gene promoters in sensory neurons. | interference with vp16-mediated activation of herpes virus immediate-early (or alpha) genes is thought to be the major cause of establishing viral latency in sensory neurons. this could be brought about by lack of a key activating transcription factor(s) or active repression. in this study we find that sensory neurons express all important components for vp16-mediated alpha gene induction, such as the pou transcription factor oct-1, host cell factor (hcf) and gabp alpha/beta. however, oct-1 and ... | 1995 | 8559654 |
| the product of a 1.9-kb mrna which overlaps the hsv-1 alkaline nuclease gene (ul12) cannot relieve the growth defects of a null mutant. | alkaline nuclease, a relatively abundant viral phosphoprotein in herpes simplex virus type 1 (hsv-1)- or hsv-2-infected cells, is encoded by a 2.3-kb mrna (r. h. costa, k. g. draper, l. banks, k. l. powell, g. cohen, r. eisenberg, and e. k. wagner, 1983. j. virol. 48, 591-603). this mrna is a member of a family of five unspliced 3'-coterminal messages. costa et al. proposed that another member of this family of mrnas (1.9-kb) may encode an n-terminally truncated protein which shares its carboxy- ... | 1996 | 8560762 |
| synthesis and antiviral effects of 2-heteroaryl substituted adenosine and 8-heteroaryl substituted guanosine derivatives. | 2-(2"- and 3"-thienyl)adenosine and the corresponding furyl derivatives were prepared though pd(0)-catalyzed coupling of 2',3',5'-tri-o-(t-butyldimethylsilyl)-2-iodoadenosine with the appropriate tributyltin derivatives followed by deprotection. preparation of the 8-(2"- and 3"-thienyl)guanosines and 8-(2"- and 3"-furyl)guanosines followed a similar route. antiviral properties of these compounds and the related 2,6-diaminopurine ribofuranosides were of no pharmacological interest. | 1995 | 8564404 |
| structural analysis of antiviral sulfated alpha-d-(1-->3)-linked mannans. | the structures of two alpha-(1-->3)-alpha-d-xylo-mannans were determined. the different antiviral activity of the xylo-mannans from nothogenia fastigiata was explained on the basis of a flexible backbone, molecular size, content and distribution of sulfate groups and of the single stubs of beta-(1-->2)-linked d-xylose. | 1995 | 8565005 |
| detection and genotyping of herpes simplex virus types 1 and 2 by polymerase chain reaction. | a simple and rapid polymerase chain reaction (pcr) procedure was developed for simultaneous detection and typing of herpes simplex virus (hsv) types 1 and 2. it was possible to detect and type hsv using two primers pairs in a simultaneous double pcr reaction, where the type of hsv present was determined on the basis of an ethidium-bromide-stained band after agarose gel electrophoresis. this pcr assay was tested on about 500 clinical specimens. | 1995 | 8569766 |
| qsar of n2-phenylguanidines as inhibitors of herpes simplex virus-1 thymidine kinase. | quantitative structure-activity relationships were developed to account for inhibition of herpes simplex virus type 1 by n2-phenylguanines. it was found that the hydrophobic and especially the steric properties of the substituents determine the relative potencies of the derivatives. | 1995 | 8584555 |
| vaccination of mice with herpes simplex virus type 1 glycoprotein d dna produces low levels of protection against lethal hsv-1 challenge. | the herpes simplex virus type 1 (hsv-1) glycoprotein d (gd) gene was inserted into vectors psvl or prc/cmv under control of the sv40 late promoter or the human cytomegalovirus major immediate-early promoter, respectively. intramuscular injection of mice with these gd-containing plasmids appeared to induce low levels of serum anti-gd antibody, as judged by the appearance of low levels of anti-hsv-1-neutralizing antibody and anti-gd elisa responses in the serum of gd-dna-vaccinated mice. as previo ... | 1995 | 8585768 |
| severity of experimentally reactivated herpetic eye disease is related to the neurovirulence of the latent virus. | the authors examined the eye diseases produced during acute and experimentally reactivated infections of rabbits intranasally inoculated with high and low neurovirulent strains of herpes simplex virus, type-1 (hsv-1). experimental reactivation of latent trigeminal ganglionic infection was accomplished by an injection of cyclophosphamide followed by one injection of dexamethasone the next day. neither drug, when given as a single injection, reactivated latent hsv-1 infection. during acute and rea ... | 1987 | 8591901 |
| mutational analysis of dna polymerase substrate recognition and subunit interactions using herpes simplex virus as prototype. | | 1995 | 8594357 |
| isolation and characterization of herpes simplex virus type 1 mutants defective in the ul6 gene. | previous studies have shown that the protein encoded by herpes simplex virus type 1 (hsv-1) gene ul6 is required for processing and packaging of replicated viral dna and is a minor component of virions and capsids. in this report, we describe the construction of ul6- hsv-1 mutants with a disrupted ul6 gene using complementing cells and show that they fail to synthesize the ul6 protein or produce infectious virus in noncomplementing cells. the mutants synthesized but failed to process and encapsi ... | 1996 | 8599195 |
| the effect of uv-b irradiation on secondary epidermal infection of mice with herpes simplex virus type 1. | previous studies have indicated that suberythemal ultraviolet b (uv-b) irradiation of c3h mice before primary infection with herpes simplex virus (hsv) type 1 does not result in increased morbidity or mortality, but a suppressed delayed type hypersensitivity (dh) to the virus can be demonstrated. any effect of uv radiation on pathogenesis during secondary epidermal hsv infection has not been previously examined. mice were immunized by subcutaneous injection of inactivated hsv and, 5 days later, ... | 1996 | 8601786 |
| immune cell infiltration in corneas of mice with recurrent herpes simplex virus disease. | reactivation of latent herpes simplex virus type 1 (hsv-1) infection was induced by uv irradiation of the corneas of latently infected mice. on days 1-4 after stimulation, infectious virus was sought in nervous and ocular tissue. on days 4, 7 and 10, eyes with either recurrent epithelial or stromal disease and appropriate controls were stained to identify immune cells and hsv-1 antigens. the maximum incidence of infectious virus was on day 2 when 5/10 ophthalmic parts of the trigeminal ganglion ... | 1996 | 8609495 |
| the novel protein kinase of the rr1 subunit of herpes simplex virus has autophosphorylation and transphosphorylation activity that differs in its atp requirements for hsv-1 and hsv-2. | the large subunit of herpes simplex virus (hsv) ribonucleotide reductase (rr1) designated icp6 and icp10 for hsv-1 and hsv-2, respectively, has a novel protein kinase (pk) enzymatic activity. icp10 is localized on the cell surface, a localization that depends on an intact transmembrane (tm) segment. we used immunocomplex pk assays to examine the pk activity of icp10 in stably transfected eukaryotic cells. activity was distinct from that of casein kinase ii (ckii) in that it did not require monov ... | 1996 | 8614985 |
| regulation of neighboring gene expression by the herpes simplex virus type 1 thymidine kinase gene. | the herpes simplex virus type 1 thymidine kinase (tk) gene (ul23) lies upstream of the gh (ul22) gene with its 3' end overlapping the gh promoter, and it overlaps the ul24 gene's regulatory and coding sequences at its 5' end in a head-to-head orientation. thus, tk expression could affect gh expression by promoter occlusion and ul24 expression by transcriptional or posttranscriptional mechanisms. to investigate these possibilities, we analyzed the effects of tk promoter mutations that reduce tk e ... | 1996 | 8615022 |
| characterization of nuclear structures in cells infected with herpes simplex virus type 1 in the absence of viral dna replication. | herpes simplex virus type 1 dna replication occurs in nuclear domains termed replication compartments, which are areas of viral single-stranded dna-binding protein (ul29) localization (m.p. quinlan, l. b. chen, and d. m. knipe, cell 36:857-868). in the presence of herpesvirus-specific polymerase inhibitors, ul29 localizes to punctate nuclear foci called prereplicative sites. using versions of the helicase-primase complex proteins containing short peptide epitopes which can be detected in an immu ... | 1996 | 8627697 |
| high-dose ocular infection with a herpes simplex virus type 1 icp34.5 deletion mutant produces no corneal disease or neurovirulence yet results in wild-type levels of spontaneous reactivation. | we report here that in the rabbit ocular model of herpes simplex virus type 1 (hsv-1) latency, spontaneous reactivation of the hsv-1 icp34.5 deletion mutant d34.5 increased significantly in response to increasing infectious doses. at the highest infectious dose of d34.5, the spontaneous reactivation rate was indistinguishable from that of wild-type virus (average spontaneous reactivation rates for d34.5, 0.3 to 1.4% at 2 x 10(5) pfu per eye, 3.4% at 2 x 10(6) pfu per eye, and 6.3 to 11.5% at 1 x ... | 1996 | 8627763 |
| structure-activity relationships among 2-substituted 5,6-dichloro-, 4,6-dichloro-, and 4,5-dichloro-1-[(2-hydroxyethoxy) methyl]- and -1-[(1,3-dihydroxy-2-propoxy) methyl]benzimidazoles. | the sodium salt of 2,5,6-trichlorobenzimidazole (8a) was condensed with [2-(benzyloxy)ethoxy]-methyl chloride (9) and [1,3-bis(benzyloxy)-2-propoxy]methyl chloride (18) to provide the corresponding protected acyclic nucleosides 10a and 19a, which on debenzylation afforded 2,5,6-trichloro-1-[(2-hydroxyethoxy)methyl]benzimidazole (11a) and 2,5,6-trichloro-1-[(1,3-dihydroxy-2-propoxy)methyl] benzimidazole (20a), respectively. a similar condensation of 2,4,6-trichlorobenzimidazole (2a) and 2,4,5-tri ... | 1996 | 8632412 |
| acute myeloblastic leukaemia presenting with herpes simplex type-1 viraemia and pneumonia. | we report a patient with acute myeloblastic leukaemia who presented with a pneumonia and herpes simplex viraemia associated with primary herpes simplex virus-1 infection. the importance of detecting and treating viral infections in haematology patients is discussed. | 1996 | 8639437 |
| comparison of the effects of three different toxin genes and their levels of expression on cell growth and bystander effect in lung adenocarcinoma. | transduction of malignant cells with toxin genes provides a novel means to promote tumor cell destruction. the efficacy of a toxin gene is dependent on the cell type targeted, the quantity of exogenous protein synthesized, and the mechanisms of growth inhibition and bystander killing. to develop gene therapy for targeting metastatic lung adenocarcinoma, the toxic activity of herpes simplex virus type 1-thymidine kinase, escherichia coli cytosine deaminase, and human deoxycytidine kinase were inv ... | 1996 | 8640820 |
| structure-function analysis of soluble forms of herpes simplex virus glycoprotein d. | glycoprotein d (gd) of herpes simplex virus (hsv) is essential for virus entry. truncated forms of gd lacking the transmembrane and cytoplasmic tail regions have been shown to bind to cells and block plaque formation. using complementation analysis and a panel of gd mutants, we previously identified four regions of gd (regions i to iv) which are important for virus entry. here, we used baculovirus vectors to overexpress truncated forms of wild-type gd from hsv type 1 (hsv-1) [gd-1(306t)] and hsv ... | 1996 | 8648717 |
| oral inoculation with herpes simplex virus type 1 infects enteric neuron and mucosal nerve fibers within the gastrointestinal tract in mice. | herpes simplex virus type 1 (hsv-1) is commonly encountered first during childhood as an oral infection. after this initial infection resolves, the virus remains in a latent form within innervating sensory ganglia for the life of the host. we have previously shown, using a murine model, that hsv-1 placed within the lumen of the esophagus gains access to nerves within the gut wall and establishes a latent infection in sensory ganglia (nodose ganglia) of the tenth cranial nerve (r. m. gesser, t. v ... | 1996 | 8648749 |
| antisense strategies and therapeutic applications. | the concepts underlying the antisense approach to disease therapy are discussed, and potential applications are examined. antisense therapeutic agents bind to dna or rna sequences, blocking the synthesis of cellular proteins with unparalleled specificity. transcription and translation are the two processes with which the agents interfere. there are three major classes of antisense agents: antisense sequences, commonly called antisense oligonucleotides; antigene sequences; and ribozymes. antisens ... | 1996 | 8653480 |
| inheritance of abnormal expression of sos-like response in xeroderma pigmentosum and hereditary cancer-prone syndromes. | the time course of induction of sos-like stress responses such as enhanced reactivation (er) and enhanced mutagenesis (em) has been investigated in uv-c-irradiated skin fibroblasts from a xeroderma pigmentosum (xp) family, using herpes simplex virus type 1 as a probe. similar er studies were performed in a li-fraumeni syndrome (lfs) family and in a family with a high incidence of breast, ovarian, and colon cancer. in two xp (complementation group b) patients, with a striking absence of skin tumo ... | 1996 | 8653707 |
| [polymerase chain reaction assay of hsv in the rapid diagnosis of encephalitis]. | | 1995 | 8654268 |
| alzheimer's disease, herpes simplex virus type 1, cold sores and apolipoprotein e4. | | 1995 | 8654779 |
| growth and cytopathogenicity of herpes simplex virus in a macrophage cell line, raw264: a good indicator of intraperitoneal pathogenicity. | macrophages are known to play a critical role in host resistance to herpes simplex virus (hsv). in this study, we investigate the interaction between various hsv strains with different virulence and a murine macrophage cell line, raw264. highly attenuated strains replicated poorly in raw264 cells and were cleared from the cultures. for the eleven viruses tested, there was good correlation between intraperitoneal pathogenicity for adult mice and replication in raw264 cells. it was also shown that ... | 1995 | 8657019 |
| prevalence of antibodies to herpes simplex virus types 1 and 2, epstein-barr virus and cytomegalovirus in teenage girls. | seropositivity to herpes virus type 1 (hsv-1), herpes virus type 2 (hsv-2), cytomegalovirus (cmv) and epstein-barr virus (ebv) was estimated in a group of 98 16-year-old swedish girls. antibodies to hsv-1 were seen in 41% of the girls and to hsv-2 in 1%, and antibodies to cmv in 45 and to ebv in 82%. in girls with coitus experience, there was significantly higher prevalence of hsv-1 and ebv antibodies, compared with girls with no sexual contact. the age of coitarche or number of coitus partners ... | 1995 | 8658062 |
| mechanisms of immunization with a replication-defective mutant of herpes simplex virus 1. | we have investigated the mechanisms by which subcutaneous immunization of mice with a replication-defective mutant of herpes simplex virus 1 protects against infection of the eye and latent infection of the trigeminal ganglion following corneal challenge. first, we have shown that immunization reduces the number of trigeminal ganglion neurons in challenged animals that express the latency-associated transcript. this indicates that the reduction in the incidence of latent infection by challenge v ... | 1996 | 8661391 |
| the human histidine decarboxylase promoter is regulated by gastrin and phorbol 12-myristate 13-acetate through a downstream cis-acting element. | transcriptional regulation of the human histidine decarboxylase (hdc) gene by gastrin and the phorbol ester phorbol 12-myristate 13-acetate (pma) was studied using transient transfection of human hdc promoter-luciferase constructs in a human gastric carcinoma cell line (ags-b) that expresses the human cholecystokinin-b/gastrin receptor. the transcriptional activity of the human hdc promoter was stimulated 3-4-fold by gastrin and 13-fold by pma, effects that could be blocked by down-regulation or ... | 1996 | 8662934 |
| the herpes simplex virus type 1 transactivator icpo mediates aberrant intracellular localization of the viral helicase/primase complex subunits. | the infected cell polypeptide 0 (icp0) protein of herpes simplex virus type 1 (hsv-1) is a promiscuous transactivator. when expressed by transfection, icp0 forms spherical structures in the nucleus. using a double-label immunofluorescence assay, we have found that the hsv-1 helicase/primase complex subunits accumulate within icp0 structures in cotransfected cells. this phenomenon was also observed in cells coexpressing icp0 and ul6, a protein thought to be involved in the cleavage and/or packagi ... | 1996 | 8669135 |
| evidence that the nuclease activities associated with the herpes simplex type 1 dna polymerase are due to the 3'-5' exonuclease. | we investigated nuclease activities associated with the catalytic subunit of herpes simplex virus type 1 dna polymerase. we confirm that a 3'-5' exonuclease copurifies with this enzyme. previous reports suggested that a 5' dnase was intrinsic to the polymerase. our preparation lacks such activity. | 1996 | 8676514 |
| [treatment of experimental herpesvirus infection with nonsteroidal anti-inflammatory agents]. | the therapeutic effect of nonsteroid antiinflammatory drugs (pyrazolone and salicylate derivatives) and their combinations with acyclovir was assessed in experimental herpesvirus infection in cell culture and in mice. a combination of 4-iodoantipyrine and acyclovir protected mice from herpesvirus encephalitis. this effect was associated with a decrease of the level of the virus in mouse brain and an increase of the titers of serum interferon and virus neutralizing antibodies. | 1996 | 8686276 |
| therapeutic potential of cidofovir (hpmpc, vistide) for the treatment of dna virus (i.e. herpes-, papova-, pox- and adenovirus) infections. | (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (hpmpc, cidofovir, vistide) is an acyclic nucleoside phosphonate with broad-spectrum activity against a wide variety of dna viruses including herpesviruses [herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), varicella-zoster virus (vzv), cytomegalovirus (cmv), epstein-barr virus (ebv), human herpesvirus type 6 (hhv-6) and equine and bovine herpesviruses], papovaviruses [human polyoma virus and human papilloma virus (hpv)], adeno-, irido-, ... | 1996 | 8701600 |
| a comparison of referral patterns and characteristics of patients with first episode symptomatic genital hsv-1 and hsv-2 infections in sheffield. | to ascertain factors associated with hsv-1 and hsv-2 isolates in patients attending a genitourinary medicine clinic with symptomatic first episode genital herpes (fegh). | 1996 | 8707325 |
| activity of penciclovir in antiviral assays against herpes simplex virus. | the effect of penciclovir and acyclovir on the replication of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) strains was determined in mrc-5 cells infected with 0.01 pfu/cell and exposed to the drugs for 72 h to allow multiple cycles of replication. penciclovir was significantly more active than acyclovir against three strains of hsv-1 and three strains of hsv-2 at 1 mg/l (p = 0.009), 3 mg/l (p < 0.001) and 10 mg/l (p = 0.001). further comparisons between the compounds were made in mrc-5 ... | 1996 | 8707740 |
| highly virulent strains of herpes simplex virus fail to kill mice following infection via gingival route. | virulence of herpes simplex virus (hsv) in mice has been demonstrated to be dependent on the site of infection. in this experiment, pathogenesis of hsv was studied in 2 different routes of infection in a mouse model system. when balb/c mice were infected with 5 x 10(3) plaque-forming units (pfu) of virulent hsv type 1 miyama gc+ strain (hsv-1-gc+) intraperitoneally, all mice were dead in 6 to 9 days. hsv-1-gc+ was recovered from organs such as the cerebrum, cerebellum, brainstem, and spleen 2 to ... | 1996 | 8708138 |
| structure--activity relationships of the didemnins. | bioactivities of 42 didemnin congeners, either isolated from the marine tunicates trididemnun solidum and aplidium albicans or prepared synthetically and semisynthetically, have been compared. the growth inhibition of various murine and human tumor cells and plaque reduction of hsv-1 and vsv grown on cultured mammalian cells were used to assess cytotoxicity and antiviral activity. biochemical assays for macromolecular synthesis (protein, dna, and rna) and enzyme inhibition (dihydrofolate reducta ... | 1996 | 8709112 |
| evidence that two latency-associated transcripts of herpes simplex virus type 1 are nonlinear. | the latency-associated transcripts (lats) of herpes simplex virus type 1 (hsv-1) are the only viral gene products that accumulate to abundant levels in latently infected cells. others have reported species of 2.0, 1.50, and 1.45 kb; only the 2.0-kb species is seen in productively infected cells, and there is evidence that it behaves as an intron. we examined the lats both in trigeminal ganglia of latently infected mice and in productively infected cultures of monkey cv-1 cells. after glyoxalatio ... | 1996 | 8709218 |
| cross-linking of glycoprotein oligomers during herpes simplex virus type 1 entry. | herpes simplex virus (hsv) has 10 glycoproteins in its envelope. glycoprotein b (gb), gc, gd, gh, and gl have been implicated in virus entry. we previously used chemical cross-linking to show that these five glycoproteins were close enough to each other to be cross-linked into homodimeric and hetero-oligomeric forms; hetero-oligomers of gb-gc, gc-gd, gd-gb, gh-gl, gc-gl and gd-gl were found in purified virions. to better understand the roles of these glycoproteins in viral entry, we have modifie ... | 1996 | 8709231 |
| inhibition of herpes simplex virus type 1 immediate-early gene expression by alpha interferon is not vp16 specific. | pretreatment of tissue culture cells with alpha interferon (ifn-alpha) inhibits the transcription of herpes simplex virus type 1 (hsv-1) immediate-early (ie) genes, an effect which has been attributed to reduced transactivation of ie promoters by the virion protein vp16. our previous demonstration that ifn-alpha inhibited the replication of the hsv-1 mutant in1814, which has a mutated vp16 unable to activate ie transcription, appeared to be incompatible with ifn-alpha having an effect on vp16 ac ... | 1996 | 8709261 |
| prolonged gene expression and cell survival after infection by a herpes simplex virus mutant defective in the immediate-early genes encoding icp4, icp27, and icp22. | very early in infection, herpes simplex virus (hsv) expresses four immediate-early (ie) regulatory proteins, icp4, icp0, icp22, and icp27. the systematic inactivation of sets of the ie proteins in cis, and the subsequent phenotypic analysis of the resulting mutants, should provide insights into how these proteins function in the hsv life cycle and also into the specific macromolecular events that are altered or perturbed in cells infected with virus strains blocked very early in infection. this ... | 1996 | 8709264 |
| in vitro establishment of lytic and nonproductive infection by herpes simplex virus type 1 in three-dimensional keratinocyte culture. | the f strain of herpes simplex virus type 1 (hsv-1) was tested for its ability to produce lytic or nonproductive infection in squamous epithelial cells cultured in a three-dimensional organotypic tissue culture. for the tissue culture, we used hacat cells (immortalized skin keratinocytes) and normal fibroblasts derived from the skin. the cultures were infected with hsv-1 (5 pfu) either when the epithelial cells had grown as a monolayer with a confluence of 80% on the collagen fibroblast gel or 3 ... | 1996 | 8709294 |
| [herpetic esophagitis in 5 immunocompetent patients]. | in five immunocompetent patients, 3 of whom were moderately ill, herpes simplex virus (hsv) oesophagitis was diagnosed. hsv oesophagitis is a frequent infection in immunocompromised hosts. nevertheless the english medical literature (medline) contains at least 33 cases of hsv oesophagitis in immunocompetent persons over the period 1983 to 1993. odynophagia, dysphagia and chest pain are common symptoms. endoscopy is necessary for diagnosis: brush cytology, biopsy for histology and viral culture a ... | 1996 | 8710027 |
| gene therapy for brain tumours. | | 1995 | 8719825 |
| herpesvirus infections of the vulva. | | 1996 | 8723819 |
| adrenocortical activation by herpes virus: involvement of il-1 beta and central noradrenergic system. | we investigated the involvement of brain cytokines and central monoamines in mediating the effect of the neurotropic herpes simplex virus-1 (hsv-1) on adrenocortical activity in rats. corneal inoculation with a neurovirulent hsv-1 strain, but not with an avirulent strain, induced interleukin-1 beta (il-1 beta) gene expression mainly in the pons and hypothalamus, and caused an elevation in serum corticosterone levels. infectious virus was isolated in low titres only from the trigeminal ganglia an ... | 1996 | 8724676 |
| restitution of the ul56 gene expression of hsv-1 hfem led to restoration of virulent phenotype; deletion of the amino acids 217 to 234 of the ul56 protein abrogates the virulent phenotype. | recently it was shown that the avirulent phenotype of hsv-1 strain hfem is correlated to the lack of dna sequences of the promoter region of the ul56 gene. in order to investigate the role of the ul56 gene of hsv-1 in the process of viral pathogenicity in more detail, a complete copy of the ul56 gene of the virulent hsv-1 strain 17 was inserted within the dna sequences of the incomplete ul56 gene of the genome of hsv-1 strain hfem. the ul56 gene of hsv-1 strain 17 comprises 1428 bp corresponding ... | 1996 | 8725118 |
| contrast-enhanced magnetization transfer saturation imaging improves mr detection of herpes simplex encephalitis. | cranial mr imaging was performed in three patients in whom herpes simplex encephalitis was subsequently proved. in all cases, the postcontrast t1 weighted mr images obtained with magnetization transfer saturation showed greater central nervous system involvement than was apparent on the conventional mr images. specifically, the postcontrast magnetization transfer images were superior at delineating generalized meningeal enhancement as well as focal areas of brain involvement not seen on noncontr ... | 1996 | 8730199 |
| papillomavirus in cervicovaginal smears of women infected with human immunodeficiency virus. | it has been described that women infected with the human immunodeficiency virus (hiv) present more frequent cytological abnormalities in cervicovaginal smears, generally related to infection by human papillomavirus (hpv). the present work is a study of cervicovaginal smears of 147 hiv-seropositive women submitted to routine gynecological examinations. the smears were stained by the papanicolaou method. cytopathic effects of hpv were found in 38 (25.8%) cases. nuclear atypias of cervical intraepi ... | 1995 | 8731285 |
| pathology of chronic herpes infection associated with seizure disorder: a report of two cases with tissue detection of herpes simplex virus 1 by the polymerase chain reaction. | although uncommon, the association of chronic encephalitis with epilepsy is well recognized. while a viral etiology has been suspected based on the morphology, to date no virus has been successfully cultured from the brain in patients with rasmussen's encephalitis. we describe the pathologic findings and report the detection of herpes simplex virus 1 (hsv1) in the brain in two patients who presented primarily with intractable seizures. in the first patient, an intrauterine infection was suspecte ... | 1995 | 8736603 |
| prokaryotic expression of an immediate-early gene of human herpesvirus 6 and analysis of its viral antigen expression in human cells. | segments of an immediate-early (1e) protein (1e03; 958 amino acids (aa)), encoded by clone psty03, of human herpesvirus 6 (hhv-6) variant b strain hst were expressed as beta-galactosidase fusion proteins in escherichia coli. using western blot analysis, and the serum of a patient having high titer anti-hhv-6 antibodies, an antigenic region of the ie03 protein was mapped between residues 340 and 505 (pue03ie-m). the fusion protein expressed in e. coli harboring plasmid pue03ie-m was purified afte ... | 1996 | 8738179 |
| correlation of in vivo topical efficacies with in vitro predictions using acyclovir formulations in the treatment of cutaneous hsv-1 infections in hairless mice: an evaluation of the predictive value of the c* concept. | the purpose of this study was to carry out an extensive examination of the c* concept for prediction of the topical antiviral efficacies of acyclovir (acv) formulations in a hairless mouse model for the treatment of cutaneous herpes simplex virus type-1 (hsv-1) infections. this method is based on estimation of the free drug concentration at the target site (c*), which is presumed to be the basal cell layer of the epidermis. five different formulations (containing 5% acv) were examined in a finit ... | 1996 | 8739606 |
| [a case of neonatal herpes infection which proved to be a mixed infection of herpes simplex virus type 1 and type 2]. | we encountered a case which proved to be a mixed infection of herpes simplex virus (hsv) type 1 and type 2 in retrospective terms by in situ hybridization (ish) and polymerase chain reaction (pcr). the case was a male. the gestational age was 39 weeks and 2 days. the birth body weight was 3024 g. a fever developed from the age of 6 days and he was admitted to the neonatal intensive care unit at the age of eight days. ast was 1042 iu/l, and alt 206 iu/l. in spite of treatment, the patient died at ... | 1996 | 8741714 |
| antiviral activity of an oligo(nucleoside methylphosphonate) that targets hsv-1 immediate-early pre-mrna 4,5 is augmented by cotreatment with replication-defective adenovirus. | replication-defective adenovirus p259a caused a 400-fold increase in the sequence-specific antiherpetic activity of oligo(nucleoside methylphosphonate) (onmp) ie4,5sa. herpes simplex virus type 1 (hsv-1) growth was not inhibited in cells exposed to p259a in the absence of ie4,5sa or in cells cotreated with ie4,5sa and heated (10 minutes, 90 degrees c) p259a virus. fluorescent microscopy of vero cells treated with bodipy-conjugated ie4,5sa revealed intracellular localization within endocytic-like ... | 1995 | 8746773 |
| simultaneous pcr detection of haemophilus ducreyi, treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers. | a multiplex pcr (m-pcr) assay with colorimetric detection was devised for the simultaneous amplification of dna targets from haemophilus ducreyi, treponema pallidum, and herpes simplex virus (hsv) types 1 and 2. by using target-specific oligonucleotides in a microwell format, 298 genital ulcer swab specimens collected in new orleans during three intervals from 1992 through 1994 were evaluated. the results of the m-pcr assay were compared with the results of dark-field microscopy and h. ducreyi c ... | 1996 | 8748271 |
| foscarnet therapy for acyclovir-resistant herpes simplex virus 1 infection in allogeneic bone marrow transplant recipients. | | 1995 | 8749652 |
| [future directions of gene therapy for lung cancer]. | failure to achieve sustained therapeutic responses with conventional medical therapies has presented a challenge to gene therapy as a new approach to lung cancer. we used an allo-major histocompatibility complex class i gene (h-2kb), and a gene encoding the thymidine kinase of herpes simplex virus type (hsv1-tk) as a drug sensitivity gene. the h-2kb gene-transfected clones of mouse tumor cells were rejected completely by syngeneic dba/2 mice. furthermore, the mice that had rejected the h-2kb gen ... | 1995 | 8752480 |