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incomplete replication of human parainfluenza virus type 4 in llc-mk2 cells and in l929 cells.human parainfluenza virus type 4a (hpiv-4a) and type 4b (hpiv-4b) were tested for their ability to replicate in the monkey kidney llc-mk2 cell line (mk2 cells) and the murine l929 cell line (l929 cells). these cells are normally non-permissive for replication of hpiv-4; however, treatment with acetylated trypsin led to virus replication in mk2 cells, but was less effective for l929 cells. endogenously produced interferon (ifn) played no role in virus replication in l929 cells. synthesis of virus ...200010917155
fusion properties of cells constitutively expressing human parainfluenza virus type 4a haemagglutinin-neuraminidase and fusion glycoproteins.we established hela cell lines that constitutively expressed the fusion (f) and/or haemagglutinin-neuraminidase (hn) glycoproteins of human parainfluenza virus type 4a (piv-4a) and used them to analyse the roles of these glycoproteins in virus-induced cell fusion. no syncytium formation occurred, even in hela cells expressing both the f and hn proteins (hela-4af+hn cells). also no syncytium was found in a mixed culture of cells expressing the f protein (hela-4af) and the hn protein (hela-4ahn). ...19947996143
molecular cloning and sequence analysis of human parainfluenza type 4a virus hn gene: its irregularities on structure and activities.we cloned the cdna of human parainfluenza type 4a virus (piv-4a) hn gene by reverse-transcription of virus-specific mrnas and genomic rna, and determined the complete nucleotide sequence of the hn gene. the predicted hn protein sequence of piv-4a showed significant relatedness with those of other paramyxoviruses, sv5, ndv, muv, piv-3, bpiv-3, indicating a common ancestor. the homologies between the viruses suggested that piv-4a is more closely related to ndv, sv5, and muv than to the parainfluen ...19902155512
virus-specific polypeptides of human parainfluenza virus type 4 and their synthesis in infected cells.we have studied the structural components of human parainfluenza virus type 4a (piv-4a) and identified some virus-specific polypeptides by immunoprecipitation with polyclonal and monoclonal antibodies followed by one- or two-dimensional sds-page. hn polypeptides existed as monomer, disulfide-linked dimer, and disulfide-linked larger oligomer in cells infected with piv-4a. interestingly, the nonreduced np, the nonreduced fusion, and the reduced f1 proteins migrated as doublets. two f1 polypeptide ...19892545032
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