evaluation of 6-azauridine and 5-iododeoxyuridine in the treatment of experimental viral infections.the potential antiviral activity of 6-azauridine and 5-iododeoxyuridine was evaluated in a coordinated study at five institutions. experimental models in five species, the mouse, rabbit, swine, cat, and ferret, were established with use of 10 viruses: herpesvirus hominis types 1 and 2, murine cytomegalovirus, vaccinia virus, shope fibroma virus, transmissible gastroenteritis virus, swine influenza virus, feline viral rhinotracheitis virus, feline panleukopenia virus, and ferret distemper virus. ...1976180189
analysis of a 9.6 kb sequence from the 3' end of canine coronavirus genomic rna.we have analysed the organization of the 3' end of the genomic rna of canine coronavirus (ccv), a virus which has a close antigenic relationship to transmissible gastroenteritis virus (tgev), porcine respiratory coronavirus (prcv) and feline infectious peritonitis virus (fipv). genomic rna isolated from ccv strain insavc-1-infected a72 cells was used to generate a cdna library. overlapping clones, spanning approximately 9.6 kb [from the 3' end of the polymerase gene, 1b, to the poly(a) tail] wer ...19921431811
proteins specified by swine transmissible gastroenteritis virus: identification of non-structural proteins by two-dimensional electrophoresis.four virus-induced non-structural proteins with apparent molecular weights of 11-14 kilodaltons (kda) were identified by two-dimensional electrophoresis in cells infected by tgev. differences in the number of non-structural proteins were observed among virulent and attenuated tgev strains as well as with two antigenically related feline and canine coronaviruses.19911662051
lesions in the small intestine of newborn pigs inoculated with porcine, feline, and canine coronaviruses.the infectivity and pathogenicity to newborn pigs of antigenically related coronaviruses from pigs (transmissible gastroenteritis virus; tgev), cats (feline infectious peritonitis virus; fipv), and dogs (canine gastroenteritis virus; cgev) were studied by light, scanning electron, and immunofluorescence microscopy. hysterectomy-derived, 12-hour-old pigs were orally given tissue culture or frozen preparations of 6 coronavirus strains (3 porcine, 2 feline, and 1 canine). the pigs were killed at re ...19816168221
antigenic relationships among homologous structural polypeptides of porcine, feline, and canine coronaviruses.transmissible gastroenteritis virus of swine (tgev), feline infectious peritonitis virus (fipv), and canine coronavirus were studied with respect to their serological cross-reactivity in homologous and heterologous virus neutralization, immune precipitation of radiolabeled tgev, electroblotting, and enzyme-linked immunosorbent assay using individual virion polypeptides prepared by polyacrylamide gel electrophoresis. tgev was neutralized by feline anti-fipv serum, and the reaction was potentiated ...19826182101
studies of enteric coronaviruses in a feline cell line.development is reported of a feline cell line which can support the growth of coronaviruses from canine (ccv), feline (fipv) and porcine (tgev) species. the cell culture has been serially transferred over 100 times and has retained its initial growth requirements, proliferative capacity and morphologic features. each virus had specific growth characteristics in this cell culture although all produced a similar cpe and plaques under agar. cross neutralization studies demonstrated a two-way relati ...19826298992
further characterization of aminopeptidase-n as a receptor for coronaviruses.we recently reported that porcine aminopeptidase-n (papn) acts as a receptor for transmissible gastroenteritis virus (tgev). in the present work, we addressed the question of whether tgev tropism is determined only by the virus-receptor interaction. to this end, different non-permissive cell lines were transfected with the porcine apn cdna and tested for their susceptibility to tgev infection. the four transfected cell lines shown to express papn at their membrane became sensitive to infection. ...19937911642
development of a nested pcr assay for detection of feline infectious peritonitis virus in clinical specimens.a diagnostic test for feline infectious peritonitis virus (fipv) infection based on a nested pcr (npcr) assay was developed and tested with fipv, feline enteric coronavirus (fecv), canine coronavirus (ccv), and transmissible gastroenteritis virus (tgev) and clinical fluid samples from cats with effusive feline infectious peritonitis (fip). the target sequence for the assay is in the s1 region of the peplomer protein e2 gene. a vaccine strain of fipv and two wild-type fipv strains tested positive ...19979041410
development and evaluation of an elisa to measure antibody responses to both the nucleocapsid and spike proteins of canine coronavirus.a rapid and reproducible enzyme linked immunosorbent assay (elisa) was developed for detection of canine coronavirus (ccv) specific antibodies directed to both the nucleocapsid (nc) and the spike (s) proteins. the coating antigen, a methanol-treated, s-protein enriched preparation, was produced by subjecting infected cells to triton x-114 detergent followed by phase separation. the sensitivity of this assay was determined by following the course of infection in dogs experimentally infected with ...19989530608
feline aminopeptidase n is a receptor for all group i coronaviruses.human coronavirus hcv-229e and porcine transmissible gastroenteritis virus (tgev), both members of coronavirus group i, use aminopeptidase n (apn) as their cellular receptors. these viruses show marked species specificity in receptor utilization as they can only use apn of their respective species to initiate virus infection. feline and canine coronaviruses are also group i coronaviruses. to determine whether feline apn could serve as a receptor for feline coronaviruses (fcovs), we cloned the cd ...19989782266
development of a nested pcr assay for the detection of canine coronavirus.a diagnostic test for canine coronavirus (ccv) infection based on a nested polymerase chain reaction (n-pcr) assay was developed and tested using the following coronavirus strains: ccv (usda strain), ccv (45/93, field strain), feline infectious peritonitis virus (fipv, field strain), transmissible gastroenteritis virus (tgev, purdue strain), bovine coronavirus (bcv, 9wbl-77 strain), infectious bronchitis virus (ibv, m-41 strain) and fecal samples of dogs with ccv enteritis. a 230-bp segment of t ...199910403671
mutational analysis of aminopeptidase n, a receptor for several group 1 coronaviruses, identifies key determinants of viral host range.feline coronavirus (fcov), porcine transmissible gastroenteritis coronavirus (tgev), canine coronavirus (ccov), and human coronavirus hcov-229e, which belong to the group 1 coronavirus, use aminopeptidase n (apn) of their natural host and feline apn (fapn) as receptors. using mouse-feline apn chimeras, we identified three small, discontinuous regions, amino acids (aa) 288 to 290, aa 732 to 746 (called r1), and aa 764 to 788 (called r2) in fapn that determined the host ranges of these coronavirus ...200717093189
molecular characterisation of the virulent canine coronavirus cb/05 strain.this paper characterises a virulent strain (cb/05) of canine coronavirus (ccov) isolated from the internal organs of pups that had died of a systemic disease without evidence of other common canine pathogens. high viral rna titres were detected in the internal organs by a real-time rt-pcr assay specific for ccov type ii. sequence analysis of the 3' end (8.7kb) of the genomic rna of strain cb/05 revealed conserved structural as well as non-structural proteins, with the exception of a truncated fo ...200717275120
recombinant canine coronaviruses related to transmissible gastroenteritis virus of swine are circulating in dogs.four canine coronavirus type ii (ccov-ii) strains were identified in the guts and internal organs of pups which had died of acute gastroenteritis. the ccov-ii strains were strictly related to porcine transmissible gastroenteritis virus (tgev) in the n-terminal domain of the spike protein, whereas in the other parts of the genome, a higher genetic relatedness to recent ccov-ii isolates was observed. experimental infection of dogs with a tgev-like isolate induced mild gastroenteritis without any s ...200919036814
immunogenicity and protective efficacy in dogs of an mf59™-adjuvanted vaccine against recombinant canine/porcine coronavirus.recently, canine coronavirus (ccov) strains with putative recombinant origin with porcine transmissible gastroenteritis virus (tgev) were shown to be widespread in europe. in this study, a killed vaccine against tgev-like ccov strains, included in the new subtype ccov-iib, was developed through inactivation with betapropiolactone and emulsification with mf59™ adjuvant. safety, immunogenicity and efficacy of the developed vaccine were evaluated in vivo. five 10-week-old beagle pups were administe ...201121272607
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