expression and secretion of interferon-alpha 1 by streptomyces lividans: use of staphylokinase signals and amplification of a neo gene.a gene coding for mature human interferon, ifn-alpha 1, fused to the expression and secretion signals of a staphylokinase gene (sak) derived from staphylococcus aureus phage 42d, was inserted into the streptomyces promoter probe vector pij487. streptomyces lividans transformed with the recombinant plasmid (pmg341) secreted biologically active ifn-alpha 1 into the culture medium. expression of the ifn-alpha 1 gene was at least on the translational level directed by the sak signals since numerous ...19882851494
purification and characterization of the bacterial plasminogen activator staphylokinase secreted by a recombinant bacillus subtilis.a gene coding for the bacterial plasminogen activator staphylokinase (sak) was cloned from staphylococcus aureus bacteriophage 42d into an exoprotease reduced mutant strain of bacillus subtilis (1). yields of up to 50 mg sak per litre of culture supernatant were obtained depending on the medium used. sak purified by ion exchange chromatography and gel filtration had a specific activity of 16,000 units/mg protein. isoelectric focusing of the purified sak revealed heterogeneity with respect to the ...19883146168
characteristics of staphylococcus aureus strains isolated from bovine mastitic milk: bacteriophage and antimicrobial agent susceptibility, and enterotoxigenicity.staphylococcus aureus strains isolated from bovine mastitic milk in trinidad were examined for their susceptibility to bacteriophages and antimicrobial agents and their ability to produce enterotoxins. phage 42d was used to screen for bovine strains of s. aureus in milk. of 250 strains tested, 224 (89.6%) were sensitive to phages in the international phage set (ips), 85 (34.0%) were resistant to antimicrobial agents and 134 (53.6%) were enterotoxigenic. strains lysed by phages in various groups ...19958553706
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