Publications

TitleAbstractYear
Filter
PMID
Filter
identification of isolate-specific sporozoite proteins of cryptosporidium parvum by two-dimensional gel electrophoresis.five isolates of cryptosporidium parvum collected from human, horse, and calf sources were compared for differences in sporozoite protein patterns by using two-dimensional gel electrophoresis. silver-stained two-dimensional gels contained over 300 protein spots from detergent-solubilized sporozoites. a distinguishing 106-kilodalton peptide that shifted in isoelectric point was detected in four of the five isolates. computerized two-dimensional gel analysis was performed to obtain objective quant ...19902365452
[spectrum of species and incidence of endoparasites in foals and their mother mares from breeding farms with and without anthelmintic prophylaxis in upper bavaria].in this epidemiological study 37 foals and mares were included, which have been coproscopically examined in regular intervals over a period lasting up to 13 months. the animals derived from nine breeding farms. in three of these farms no anthelmintics were used before this investigation, whereas in six farms at least three years before regular prophylactic treatments with anthelmintics have been carried out. this investigation revealed no differences in the species spectrum of endoparasites as w ...19968720956
detection of cryptosporidium parvum in horses: thresholds of acid-fast stain, immunofluorescence assay, and flow cytometry.feces collected from three asymptomatic horses and seeded with cryptosporidium parvum oocysts (10(1) to 10(6)/g of feces) were evaluated by acid-fast staining (af), an immunofluorescent antibody (ifa) technique, and flow cytometry. the thresholds of detection were 5 x 10(5) oocysts/g of feces for the ifa and af techniques and 5 x 10(4) oocysts/g for flow cytometry.19999889243
method for detection and enumeration of cryptosporidium parvum oocysts in feces, manures, and soils.eight concentration and purification methods were evaluated to determine percentages of recovery of cryptosporidium parvum oocysts from calf feces. the nacl flotation method generally resulted in the highest percentages of recovery. based on the percentages of recovery, the amounts of fecal debris in the final oocyst preparations, the relatively short processing time (<3 h), and the low expense, the nacl flotation method was chosen for further evaluation. extraction efficiency was evaluated by u ...199910388670
dna fingerprinting of cryptosporidium parvum isolates using amplified fragment length polymorphism (aflp).the genetic variability of 10 cryptosporidium parvum isolates of human and animal origin was investigated using amplified fragment length polymorphism (aflp). analysis of fluorescent dye-labeled amplified products was carried out using an abi prisms 377 dna sequencer and abi prisms genescan software. one-hundred and twelve primer combinations were evaluated using a single c. parvum isolate. the patterns generated were highly reproducible. for subsequent study, a subset of 9 primer pairs that yie ...200010958466
identification of cryptosporidium parvum genotype 2 in domestic horses. 200515675524
detection and species identification of cryptosporidium from taiwan feeding animals.in this study, 107 fecal specimens were collected from 40 sampling sites in taiwan livestock and avian farms to test for cryptosporidium spp. oocysts. ten of 107 samples analyzed by enzyme-linked immunosorbent assay showed the presence of cryptosporidium spp., among which 6 samples were simultaneously confirmed by immunofluorescence assay and polymerase chain reaction. nucleic acid sequencing of the 18s rrna gene identified 3 clusters of cryptosporidium spp. three cryptosporidium parvum isolates ...200818372647
Displaying items 1 - 7 of 7