TitleAbstractYear(sorted ascending)
cultural and biochemical criteria for the identification of haemophilus spp from swine.twenty-two haemophilus cultures of types prevalent in swine and of different geographic origins were subjected to biochemical and cultural examinations. three subgroups were identified: one was unrease-positive, produced porphyrin from delta-aminolevulinic acid, and grew on infusion mediums supplemented only with v factor; the 2nd was unrease-negative, porphyrin-positive, and grew only on serum-enriched mediums with added v factor; and the 3rd was unrease-negative, porphyrin-negative, and grew o ...1977189648
haemophilus parasuis infection in swine.septicemic disease occurred in 49 of 126 pigs several days after being transported 80 km. all affected pigs died. the main changes in acutely affected pigs were skin discoloration, pulmonary edema, arthritis, meningitis, and renal glomerular thrombosis. in peracute cases, gross findings were minimal. haemophilus parasuis was isolated from multiple organ sites in most affected pigs. haemophilus parasuis was isolated from nasal swab specimens from 17 of 20 clinically normal pigs on the farm of ori ...1977914694
dilution technique for isolation of haemophilus from swine lungs collected at slaughter.a total of 307 lungs obtained from a slaughterhouse were cultured by a dilution technique for the isolation of haemophilus spp. the technique consisted of performing serial (10-fold) dilutions of the tissue samples to a dilution of 10(-5). two selective media were used. l broth consisted of a basal brain heart infusion broth containing 5% horse serum, 5% yeast extract, and 100 micrograms of nad and 0.5 microgram of lincomycin per ml. l-b broth was identical to l broth, except 1.5 microgram of ba ...19836350343
defining the metabolic and growth responses of porcine haemophili to exogenous pyridine nucleotides and precursors.a variety of biologically important pyridine nucleotides and precursors were examined for their capacities to satisfy the v-factor requirement of 30 strains of porcine haemophili. of the compounds tested, only nad, nmn and nicotinamide riboside (nr) supported the growth of all strains; nadp supported the growth of only the type strain of haemophilus parasuis. further studies with the h. parasuis type strain and the neotype strain of h. pleuropneumoniae demonstrated that, during growth, these org ...19862942635
some antigenic properties of haemophilus parasuis and a proposal for serological classification.we propose a serological classification of haemophilus parasuis into at least five serovars, using an agar-gel-precipitation test with extracts from autoclaved cells. thirty-two strains were examined, and it was possible to classify 26 of them. the specific antigens were thermostable and soluble and were not affected by pronase treatment but could be extracted by phenol, suggesting a polysaccharide. this polysaccharide seemed to be identical with the capsular substance of serovars 1, 2, and 3. i ...19863086374
preliminary assessment of a haemophilus parasuis bacterin for use in specific pathogen free swine.a whole cell formalin killed trivalent haemophilus parasuis bacterin was tested for efficacy in four week old, weaned specific pathogen free pigs challenged under laboratory conditions. the vaccine contained three field strains of h. parasuis selected from confirmed cases of glasser's disease. two different formulations were evaluated in separate trials. in trial 1, ten pigs received 5 ml of bacterin subcutaneously in the neck, followed by a second 5 ml dose two weeks later. another ten pigs ser ...19892531627
characterization of haemophilus parasuis isolated in japan. 19902143547
vaccination of gnotobiotic primary specific pathogen-free pigs against haemophilus parasuis.three trials were conducted to establish if young primary specific pathogen free (spf) pigs could be protected from glasser's disease by vaccination. three age groups of cesarean-derived isolator-reared gnotobiotic pigs were vaccinated twice at 4 and 6, 3 and 5, and 2 and 4 wk of age respectively with a formalin killed aluminum hydroxide adsorbed bacterin prepared from three strains of haemophilus parasuis isolated from ontario pigs affected with glasser's disease. when challenged two weeks late ...19911832078
[occurrence and drug resistance of bacteria pathogenic to the lungs from autopsy material of swine].eight hundred and fifty-four piglets which died or were euthanized due to pneumonia or rhinitis atrophicans, were investigated during the period of 1986-1990. of the animals, 569 showed bronchopneumonia, 218 had pleuritis, pericarditis and peritonitis, 165 had rhinitis atrophicans, 58 pleuropneumonia, and 9 animals had fibrinous pneumonia. pasteurella multocida, haemophilus parasuis, bordetella bronchiseptica, actinobacillus pleuropneumoniae and pasteurella haemolytica were isolated in 59.1%, 29 ...19921481214
an investigation of enzootic glasser's disease in a specific-pathogen-free grower-finisher facility using restriction endonuclease analysis.enzootic glassers's disease was investigated to study the epidemiology of the disease strains on a farm where it presented a problem. restriction endonuclease fingerprinting (ref) analysis technique was used, as all strains of haemophilus parasuis are biochemically similar and many strains are biochemically untypable. after young weaned pigs were moved from farm a to farm b, glasser's disease routinely occurred despite the use of antibiotics and a commercial bacterin. isolates were taken from th ...199317424269
optimalization of the detection of nad dependent pasteurellaceae from the respiratory tract of slaughterhouse pigs.nad dependent members of the family pasteurellaceae were cultured from the nasal cavity, surface and cut surface of the tonsils, and from the apical and caudal lobes of the lungs of 303 slaughterhouse pigs from 5 different herds in order to obtain information on the ecology of these bacteria. the specimens were plated on two different selective agar media using a special dilution technique that resulted in a good separation of individual colonies. bacteriological results were compared with serol ...19938273273
[the lipopolysaccharide structure of haemophilus parasuis strains in sds-page].the lps patterns of 231 h.p. strains were studied by using sds-page. the strains were isolated from the nasal mucous membrane of clinical healthy animals, from animals with gk and from animals with pneumonia without any symptoms of gk. the lps patterns of h.p. strains consists of 2 to 4 bands of high electrophoretical mobility. in all it was possible to distinguish seven different lps electrophoretic profiles. the distribution of the h.p. isolates from clinically healthy animals and animals with ...19947993342
characterisation of porcine haemophili isolated from australian pigs between 1988 and 1992.a total of 362 haemophili, isolated pigs throughout australia, were characterised by phenotypic properties. most were identified as actinobacillus pleuro-pneumoniae (296 isolates) or haemophilus parasuis (52 isolates). the remaining isolates were identified as haemophilus taxon 'minor group' (12 isolates) and haemophilus taxon d (two isolates). all 296 a pleuropneumoniae isolates were serotyped by slide agglutination and/or gel diffusion, using rabbit antisera against all 12 recognised serovars. ...19958787520
isolation of the major outer-membrane protein of actinobacillus pleuropneumoniae and haemophilus parasuis.a polyclonal antibody against the 35 kda major outer-membrane protein of pasteurella multocida cross-reacted with the 40 kda major outer-membrane protein of actinobacillus pleuropneumoniae and the 42 kda major outer-membrane protein of haemophilus parasuis. the n-terminal amino-acid sequences of these proteins revealed a strong homology with the putative 35 kda porin protein of pasteurella multocida (66.7 and 76.2%, respectively). significant homologies were also evident between the 40 kda and t ...19957483902
pathogenicity of haemophilus parasuis serovars 4 and 5 in contact-exposed pigs.the pathogenicities of haemophilus parasuis strains sw124 (serovar 4) and nagasaki (serovar 5) were examined by contact-exposure of specific pathogen-free (spf) pigs. ten pigs were divided into three groups. two of four pigs in the first group were inoculated intranasally (in) with 2 x 10(8) cfu of strain sw124, and the other two pigs were mingled with these in-exposed ones. all the four pigs were subclinically infected in this group. the four pigs of the second group were likewise exposed to st ...19968811627
evaluation of a pcr for detection of actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils.a pcr for the detection of actinobacillus pleuropneumoniae was evaluated. all of 102 field, isolates of a, pleuropneumoniae reacted in the pcr by amplification of a 985 bp product. no pcr amplification product was observed when examining strains of a. ureae, a. capsulatus, a. hominis, a. equuli, a, rossii, a. suis, escherichia coli, bordetella bronchiseptica. streptococcus suis, pasteurella haemolytica, pasteurella multocida, haemophilus parasuis, haemophilus taxon minor group, haemophilus taxon ...19968828126
porcine reproductive and respiratory syndrome virus (prrsv) interaction with haemophilus parasuis.the interaction of bacteria and virus has been well demonstrated in the pathogenesis of respiratory disease in swine. the interaction between porcine respiratory and reproductive syndrome virus (prrsv) and haemophilus parasuis has not been studied. we initiated studies to evaluate a possible effect of the prrsv on the pathogenesis of polyserositis caused by h. parasuis. a group of 30 three week old piglets were distributed in 4 groups. group i (10 pigs) was inoculated with prrsv and h. parasuis. ...19979220620
analysis of haemophilus parasuis by multilocus enzyme electrophoresis.the diversity among 40 australian isolates and eight reference strains of haemophilus parasuis was examined using multilocus enzyme electrophoresis. thirty-four electrophoretic types (ets) were recognized with a mean diversity per locus of 0.405. one australian isolate was located in an et separated by a considerable distance (> 0.8) from the rest of the isolates, suggesting that it may represent a different species or subspecies. the remaining 33 ets formed two distinct divisions (a and b), sep ...19979228688
neuraminidase (sialidase) activity of haemophilus parasuis.neuraminidase (sialidase), a potential virulence factor in bacteria, was demonstrated in haemophilus parasuis, an invasive swine pathogen, but not in four other pathogens of the pasteurellaceae family: h. influenzae, h. somnus, h. paragallinarum, or actinobacillus pleuropneumoniae. h. parasuis neuraminidase had an acidic ph optimum and a specificity for several substrates also cleaved by other bacterial neuraminidases. similar to the neuraminidase of pasteurella multocida, h. parasuis neuraminid ...19979231420
association of tumour necrosis factor and acute phase reactant changes with post arrival disease in swine.severe weight loss in the absence of respiratory, enteric or systemic clinical disease or gross pathologic lesions is often observed when immunologically naive boars are placed in conventional health swine facilities. affected animals develop this weight loss in spite of receiving pre-entry vaccinations against common swine pathogens, such as haemophilus parasuis or mycoplasma hyopneumoniae. in many cases, the weight loss is non-responsive to long term antibiotic therapy. in order to determine t ...19979323928
[bronchoalveolar lavage on pig breeding farms].in 182 pigs lung lavage was performed using a endotracheal tube and a catheter. the collected bronchoalveolar lavage fluid (balf) was examined microbiologically. with decreasing numbers alpha-hämolytic streptococci, bordetella bronchiseptica, haemophilus parasuis, pasteurella multocida were cultured. actinobacillus pleuropneumoniae was isolated from 3 balfs. in one farm piglets were lavaged routinely for monitoring of the lung health status.19979410726
[occurrence of bacterial infectious agents in pathologically/anatomically altered lungs of pigs and compilation of resistance spectra].the goal of this work was to investigate the type and etiology of bacterial porcine pneumonia by analyses of autopsy findings made in the tierärztlichen ambulanz schwarzenbek, aussenstelle der freien universität berlin, and in the institut für tiergesundheit, milchhygiene und lebensmittelqualität der landwirtschaftskammer westfalen-lippe in münster in the years from 1991 to 1993. the evaluation of the results of the total 6560 autopsies (n = 6560) of pigs shows: 1. pneumonia as the main diagnosi ...19989639952
effect of porcine reproductive and respiratory syndrome virus infection on the clearance of haemophilus parasuis by porcine alveolar macrophages.porcine reproductive and respiratory syndrome virus (prrsv) infection in young piglets is frequently associated with secondary infection due to various pathogens, especially those of the respiratory tract. one of the most important mechanisms in respiratory diseases is related to the alteration of function of porcine alveolar macrophages (pams). the objective of this study was to determine how prrs virus infection affects the capabilities of pams in the phagocytosis and destruction of haemophilu ...19989798089
porcine reproductive and respiratory syndrome virus and haemophilus parasuis antigen distribution in dually infected pigs.immunohistochemical, viral and bacterial isolation techniques were used to study the distribution and localization of porcine reproductive and respiratory syndrome virus (prrsv) and haemophilus (h.) parasuis in experimentally infected pigs. thirty pigs seronegative to prrsv and h. parasuis were divided into four groups. group a pigs (10 animals) were inoculated with both virus and bacteria; group b pigs (10 animals) were inoculated with bacteria, group c pigs (five animals) were inoculated with ...199910063534
biological activities of lipopolysaccharides extracted from porcine vaccine strains.lipopolysaccharides (lpss) were purified from actinobacillus pleuropneumoniae serotype 2, bordetella bronchiseptica and haemophilus parasuis serotype 5, which were used for vaccine production in japan, by the phenol-water procedure. in sds-page analysis, a. pleuropneumoniae lps, as well as escherichia coli lps, demonstrated a typical ladder profile of a smooth-type lps. on the other hand, b. bronchiseptica and h. parasuis lpss lacked the ladder profiles. it was found that the biological activity ...199910651044
establishment, validation and use of the kielstein-rapp-gabrielson serotyping scheme for haemophilus produce antisera to the 15 recognised reference strains of the kielstein-rapp-gabrielson (krg) serotyping scheme for haemophilus parasuis, validate those sera and use them to serotype 46 australian field isolates of h parasuis.200010860155
in vitro susceptibility of porcine respiratory pathogens to tilmicosin.bacterial isolates obtained from swine with various clinical diseases were tested for susceptibility to tilmicosin by minimum inhibitory concentration (mic) and kirby-bauer disk diffusion tests using national committee on clinical laboratory standards methodology. the tilmicosin mic90 was < or =0.125 microg/ml for erysiopelothrix rhusiopathiae, < or = 1 microg/ml for haemophilus parasuis isolates, 8 microg/ml for actinobacillus suis and pasteurella multocida type a, 16 microg/ml for toxigenic an ...200011108454
a cross-protection experiment in pigs vaccinated with haemophilus parasuis serovars 2 and 5 bacterins, and evaluation of a bivalent vaccine under laboratory and field conditions.cross-protection between haemophilus parasuis serovars 2 and 5 was examined in pigs using a bacterin based vaccine, and subsequently the safety and efficacy of a bivalent vaccine were evaluated. upon intratracheal challenge of a serovar 2 or 5 strain, pigs immunized with a monovalent vaccine were protected against challenge with a homologous serovar strain, but not with a heterologous serovar strain. immunization with a bivalent vaccine containing both serovars 2 and 5 bacterins conferred protec ...200111411491
the effect of a homologous bacterin given to sows prefarrowing on the development of glässer's disease in postweaning pigs after i.v. challenge with haemophilus parasuis serotype 5.the trial was carried out to evaluate the impact of maternal antibodies on the development of glässer's disease after i.v. exposure of weaned pigs with a homologous serovar of haemophilus parasuis (hps). two groups of weaned pigs were formed. group one vi (n = 10): born to vaccinated sows, weaners i.v. challenged one week postweaning and euthanatized 14 days postweaning. group two nvi (n = 10 wearners): born to non vaccinated sows, i.v. challenged one week postweaning euthanatized 14 days postwe ...200212125173
antibodies to selected viral and bacterial pathogens in european wild boars from southcentral spain.serum samples from 78 european wild boars (sus scrofa) harvested during the 1999-2000 hunting season were tested for antibodies to brucella spp., classical swine fever virus, erysipelothrix rhusiopathiae, haemophilus parasuis, leptospira interrogans serovar pomona, mycoplasma hyopneumoniae, pseudorabies virus (prv), porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus, salmonella serogroups b, c, and d, streptococcus suis, and swine influenza virus (siv) serotypes h1n1 a ...200212238391
the effect of season and vaccination for glässer's disease and post-weaning colibacillosis in an outdoor pig unit endemically infected with virulent strain of haemophilus parasuis serotype 5 and pathogenic escherichia coli.the objective of this field trial was to determine if vaccination against haemophilus parasuis serovar 5 (hps 5) and pathogenic serotypes of escherichia coli would improve nursery pig performance in an outdoor unit in different seasons. the unit was concurrently infected with hps 5 and with different serotypes of e. coli. all piglets were born to hps 5 vaccinated sows. the trial was carried out in four (two summer and two winter) groups. group 1 (e. coli and hps vaccinated, summer season) (n = 3 ...200212485355
typing of haemophilus parasuis strains by pcr-rflp analysis of the tbpa gene.on the basis of a species-specific pcr assay, a rflp analysis for typing of haemophilus parasuis strains was developed and evaluated. amplification was based on the gene tbpa, encoding a transferrin-binding protein. rflp analysis of the 1.9-kb tbpa-amplicon using taqi, avai and rsai endonucleases produced 12 different patterns for the reference strains of the 15 known h. parasuis serovars, and showed a high heterogeneity (33 rflp groups) for 101 h. parasuis clinical isolates tested. the sensitiv ...200312523987
purification and renaturation of membrane neuraminidase from haemophilus parasuis.haemophilus parasuis, which causes polyserositis, polysynovitis, meningitis, septicemia, and pneumonia in pigs, has emerged as an increasing problem in modern swine production systems. co-factors for and the pathogenesis of h. parasuis disease are not defined. one of the potential virulence factors of h. parasuis is its neuraminidase (sialidase). while purifying the h. parasuis neuraminidase from the membrane fraction, we developed a protocol to renature enzymatic activity after enzyme preparati ...200312591209
characterization of the diversity of haemophilus parasuis field isolates by use of serotyping and characterize the genetic diversity of haemophilus parasuis field isolates with regard to serovar, herd of origin, and site of isolation.200312693533
levels of nicotinamide adenine dinucleotide in extracellular body fluids of pigs may be growth-limiting for actinobacillus pleuropneumoniae and haemophilus parasuis.during infection, nutrient deprivation can alter bacterial phenotype. this, in turn, may have implications for pathogenesis and prophylaxis. actinobacillus pleuropneumoniae (biotype 1) and haemophilus parasuis, respiratory tract pathogens of swine, are both v-factor-dependent. the concentrations of v factor in the extracellular fluids of pigs are unknown and may limit the growth of these bacteria in vivo. the aim of this study was to determine the concentrations of nicotinamide adenine dinucleot ...200312889731
antimicrobial susceptibility of haemophilus parasuis and histophilus somni from pigs and cattle in denmark.a total of 52 haemophilus parasuis and 80 histophilus somni isolates were tested for antimicrobial susceptibility by mic-determinations. none of the isolates were resistant to ampicillin, ceftiofur, ciprofloxacin, erythromycin, florphenicol, penicillin, spectinomycin, tetracycline, tiamulin, or tilmicosin. two h. parasuis isolates were resistant to trimethoprim + sulfamethoxazole. six h. parasuis isolates had reduced susceptibility (0.06-0.5 microg/ml) to ciprofloxacin and 10 reduced susceptibil ...200415172697
[well attended symposium about prrs and haemophilus infection]. 200415622898
microarray analysis of haemophilus parasuis gene expression under in vitro growth conditions mimicking the in vivo environment.haemophilus parasuis is the causative agent of polyserositis in pigs, a mostly fatal disease on the rise especially in early-weaned pigs and in pig herds with a high-health status. the mechanisms by which h. parasuis propagates through the body and colonizes the serous membranes are unknown. we have used an h. parasuis microarray to identify virulence genes involved in host adaptation. h. parasuis gene expression was analysed under in vitro growth conditions mimicking the environmental condition ...200516144750
a serological survey of selected pathogens in wild boar in slovenia.serum samples collected from 178 shot wild boars (sus scrofa) were tested for the presence of antibodies against classical swine fever virus, aujeszky's disease virus (adv), porcine reproductive and respiratory syndrome virus, porcine respiratory coronavirus (prcv), transmissible gastroenteritis virus, swine influenza virus, porcine parvovirus (ppv), swine vesicular disease virus, actinobacillus pleuropneumoniae (app), mycoplasma hyopneumoniae, salmonella spp., brucella spp. and haemophilus para ...200616460352
porcine mannan-binding lectin a binds to actinobacillus suis and haemophilus parasuis.various collagenous lectins involved in innate immunity bind to surface oligosaccharides of bacteria and other microorganisms. we have been characterizing porcine plasma lectins that bind in a carbohydrate-dependent manner to surfaces of important bacterial pig pathogens including actinobacillus suis (as), a. pleuropneumoniae (app), and haemophilus parasuis (hp). a plasma protein with 32kda subunits (pi 5.4 and 5.75) bound most isolates of hp, as, and some app. partial amino acid sequences of th ...200616480769
genotypic diversity of haemophilus parasuis field strains.haemophilus parasuis is the cause of glässer's disease and other clinical disorders in pigs. it can also be isolated from the upper respiratory tracts of healthy pigs, and isolates can have significant differences in virulence. in this work, a partial sequence from the 60-kda heat shock protein (hsp60) gene was assessed as an epidemiological marker. we analyzed partial sequences of hsp60 and 16s rrna genes from 103 strains of h. parasuis and other related species to obtain a better classificatio ...200616751506
isolation and characterization of the p5 adhesin protein of haemophilus parasuis serotype 5.haemophilus parasuis is a gram-negative respiratory pathogen of young pigs that colonizes the upper respiratory tract and produces a number of symptoms collectiviely described as glässer's disease. recently, an h. parasuis p5-like outer membrane adhesin protein homologous to h. influenzae p5 was identified. the p5 adhesin was partially purified by anion exchange and size-exclusion chromatography. final purification for functional studies was performed by elution of the protein from a polyacrylam ...200616971306
muco-cutaneous candidiasis in two pigs with postweaning multisystemic wasting two distinct commercial swine herds, poor weight gain and an increased number of animals showing wasting were observed among nursery and growing pigs. cases of postweaning multisystemic wasting syndrome (pmws) and infection with haemophilus parasuis had been previously diagnosed in these herds. one growing wasted pig from each herd was necropsied and showed enlarged lymph nodes. pseudomembranous material adhered to the dorsum of the tongue, soft and hard palate in case 1, and in case 2, fibri ...200615955715
first isolation of haemophilus parasuis and other nad-dependent pasteurellaceae of swine from european wild boars.haemophilus parasuis is a colonizer of the upper respiratory tract of pigs and the etiological agent of glässer's disease, which is characterized by a fibrinous polyserositis, meningitis and arthritis. glässer's disease has never been reported in wild boar (sus scrofa), although antibodies against h. parasuis have been detected. the goal of this study was to confirm the presence of this bacterium in wild boar by bacterial isolation and to compare the strains to h. parasuis from domesticated pigs ...200717574352
transmission of pathogenic respiratory bacteria to specific pathogen free pigs at slaughter.the purpose of this study was to evaluate the transmission of pathogenic respiratory bacteria to thirteen 5-month-old specific pathogen free (spf) pigs, during the slaughtering process in a commercial slaughterhouse. before transportation, the spf pigs and the lorry were checked to confirm the absence of pathogenic respiratory bacteria. nine spf pigs (group 1) were in contact in a conventional slaughterhouse with finishing pigs, during 4h before slaughtering. four spf pigs (group 2) were slaught ...200818242013
improving pig health through genomics: a view from the is one of the most important contributors to animal welfare, productivity and profitability in pig production today. for the past 30 years, pig breeders have focused on genetic improvement of lean growth, feed efficiency, meat quality and reproduction. however, in recent years, selection objectives have been broadened to include livability, robustness and disease resistance. a dna marker for selection of resistance to f18+ e. coli has been available for several years. this marker decrease ...200818817284
studies on the interactions of haemophilus parasuis with porcine epithelial tracheal cells: limited role of los in apoptosis and pro-inflammatory cytokine release.haemophilus parasuis colonizes the upper respiratory tract of swine and causes glässer's disease. we recently demonstrated that h. parasuis can adhere to newborn pig tracheal (nptr) cells. however, the molecular mechanisms involved in upper respiratory tract colonization by h. parasuis are unknown. the aim of this work was to investigate the role of h. parasuis lipooligosaccharide (los) in bacterial adhesion to nptr cells, the ability of the bacteria and its los to induce nptr cells apoptosis, a ...200919013513
molecular cloning, sequencing and expression of the outer membrane protein a gene from haemophilus parasuis. 200919135811
cytokine expression in colostrum-deprived pigs immunized and challenged with haemophilus parasuis.the expression of several cytokines in spleen, pharyngeal lymph nodes, lung and brain after different immunization procedures and a challenge with 5 x 10(9) cfu of haemophilus parasuis was compared. five groups of colostrum-deprived pigs were used: vaccinated with (i) a bacterin, (ii) an outer-membrane-protein-vaccine, (iii) a recombinant transferring-binding protein b, (iv) exposed to a total dose of 10(5) cfu, and (v) not previously immunized. all pigs in groups iii and v died, while all anima ...200919181353
understanding haemophilus parasuis infection in porcine spleen through a transcriptomics approach.haemophilus parasuis (hps) is an important swine pathogen that causes glässer's disease, which is characterized by fibrinous polyserositis, meningitis and arthritis. the molecular mechanisms that underlie the pathogenesis of the disease remain poorly understood, particularly the resistance of porcine immune system to hps invasion. in this study, we investigated the global changes in gene expression in the spleen following hps infection using the affymetrix porcine genechip.200919196461
identification and characterization of novel immunogenic outer membrane proteins of haemophilus parasuis serovar 5.haemophilus parasuis is the aetiological agent of glässer's disease, which is responsible for cases of fibrinous polyserositis, polyarthritis and meningitis in young pigs. to develop more effective vaccines, an immunoproteome-based approach was used to analyze the outer membrane proteins of h. parasuis serovar 5. a total of 15 proteins with high immunogenicity were identified and all were showed to be immunogens for the first time in h. parasuis. further analyses of 8 selected proteins revealed ...200919576561
characterization and comparative analysis of the genes encoding haemophilus parasuis outer membrane proteins p2 and p5.haemophilus parasuis is a swine pathogen of significant industry concern, but little is known about how the organism causes disease. a related human pathogen, haemophilus influenzae, has been better studied, and many of its virulence factors have been identified. two of these, outer membrane proteins p2 and p5, are known to have important virulence properties. the goals of this study were to identify, analyze, and compare the genetic relatedness of orthologous genes encoding p2 and p5 proteins i ...200919633080
compatibility of a combined vaccination against haemophilus parasuis and porcine reproductive and respiratory syndrome virus.objective of this study was to assess the tissue compatibility of a vaccination, combining vaccines against haemophilus parasuis and porcine reproductive and respiratory syndrome virus (prrsv). clinical examinations were done with special respect to local tissue reactions after injection of 2 ml each of either porcilis glasser, or porcilis prrs (both intervet, unterschleissheim, germany), or a mixture of the two vaccines. animals were euthanized on day six post vaccination. clinical as well as g ...200919863006
correlation between clinico-pathological outcome and typing of haemophilus parasuis field strains.haemophilus parasuis is the etiologic agent of glässer's disease in pigs, which is pathologically characterized by serofibrinous polyserositis and arthritis. h. parasuis include virulent and non-virulent strains and confirmation of virulence in h. parasuis is still dependent on experimental reproduction of the disease. since the variability in virulence is supported by serotyping and genotyping (particularly, multilocus sequence typing [mlst]), we examined the relationship between the classifica ...201019945233
virulence-associated trimeric autotransporters of haemophilus parasuis are antigenic proteins expressed in vivo.glässer's disease is a re-emerging swine disease characterized by a severe septicaemia. vaccination has been widely used to control the disease, although there is a lack of extended cross-protection. trimeric autotransporters, a family of surface exposed proteins implicated in host-pathogen interactions, are good vaccine candidates. members of this family have been described in haemophilus parasuis and designated as virulence-associated trimeric autotransporters (vtaa). in this work, we produced ...201019995512
identification of putative virulence-associated genes of haemophilus parasuis through suppression subtractive hybridization.haemophilus parasuis is the causative agent of glässer's disease. up to now 15 serovars of h. parasuis have been identified, with significant differences existing in virulence between serovars. in this study, suppression subtractive hybridization (ssh) was used to identify the genetic difference between nagasaki (h. parasuis serovar 5 reference strain, highly virulent) and sw114 (h. parasuis serovar 3 reference strain, non-virulent). a total of 191 clones were obtained from the ssh library. usin ...201020171024
antibiotic susceptibility of bacteria isolated from respiratory tract of pigs in poland between 2004 and 2008.antibiotic susceptibility of bacteria isolated from nasal swabs and lungs of pigs, to 16 commonly used antibiotics, was determined by disc diffusion test. beta-lactams showed the best activity against streptococcus suis (s. suis) (> 99% of susceptible strains). the lowest sensitivity of s. suis was evidenced to: tylosin, tetracycline and neomycin (50%, 40% and 25%, respectively). isolates of escherichia coli (e. coli) demonstrated the highest susceptibility to cephalosporin (85% strains), gentam ...201021077428
porcine s100a8 and s100a9: molecular characterizations and crucial functions in response to haemophilus parasuis infection.s100 calcium-binding protein a8 (s100a8) and s100 calcium-binding protein a9 (s100a9) are pivotal mediators of inflammatory and protective anti-infection responses for the mammalian host. in this study, we present the molecular cloning of porcine s100a8 (ps100a8) and porcine s100a9 (ps100a9). both genes comprise 3 exons and 2 introns and are located on pig chromosome 4q21-q23 (closely linked to sw512). homology comparison to other mammalian species affirmed that critical functional amino acids f ...201021185856
identification of potentially virulent strains of haemophilus parasuis using a multiplex pcr for virulence-associated autotransporters (vtaa).haemophilus parasuis is the aetiological agent of glässer's disease and is also a commensal of the upper respiratory tract of pigs. trimeric autotransporter (vtaa) genes have been identified in h. parasuis and divided into three groups on the basis of the translocator domain sequence. in this study, group 3 vtaa genes were demonstrated by pcr in all 157 h. parasuis isolates tested. group 1 vtaa genes were associated with virulent strains; 52/54 (96%) group 1 vtaa negative field isolates were iso ...201121247786
molecular characterization of caveolin-1 in pigs infected with haemophilus parasuis.caveolin-1 (cav1) plays a critical role in the invasion of pathogenic microbes into host cells, yet little is known about porcine cav1. in this study, we provide the molecular characterization of cav1 in pigs following stimulation with lps/polyinosinic-polycytidylic acid as well as during infection with haemophilus parasuis. the porcine cav1 gene is 35 kb long and is located at ssc18q21; two isoforms (cav1-a and cav1-ß) are produced by alternative splicing. three point mutations were identified ...201121282513
passive immunisation of post-weaned piglets using hyperimmune serum against experimental haemophilus parasuis infection.the protective role of hyperimmune serum in the prevention of haemophilus parasuis infections in post-weaned piglets was assessed by experimental challenge. the hyperimmune serum was obtained from a pig vaccinated with a commercial vaccine against glässer's disease. thirty-eight weaned piglets were divided into four groups: three groups were immunised intramuscularly with 10ml of hyperimmune serum and one group consisted of unimmunised control animals. all piglets were subsequently infected intr ...201121295806
characterization of haemophilus parasuis isolated from brazilian swine through serotyping, aflp and pfge.haemophilus parasuis infection in pigs is characterized by fibrinous polyserositis, arthritis and meningitis. despite the fact that traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, molecular-based methods are alternatives for species-specific tests and epidemiological studies. the aim of this study was to characterize h. parasuis field strains from different states of brazil, employing serotyping and genotyping methods. serotyping revealed that ...201121529864
effects of fut1 gene mutation on resistance to infectious disease.alpha-(1,2)-fucosyltransferase (fut1) gene has been identified as a candidate gene for regulating the expression of escherichia coli f18 receptor gene (ecf18r) which promotes adherence of enterotoxigenic (etec) and verotoxigenic (vtec) escherichia coli (e. coli) via f18 fimbriae. in order to illustrate the polymorphisms of fut1 and their effects on resistance to natural infection by porcine respiratory and reproductive symdrome virus (prrsv) and haemophilus parasuis, the distributions of differe ...201121695432
haemophilus parasuis exhibits iga protease activity but lacks homologs of the iga protease genes of haemophilus influenzae.haemophilus parasuis, the bacterium responsible for gl+ñsser's disease, is a pathogen of significant concern in modern high-health swine production systems but there is little information regarding the identity or function of its virulence factors. several important human mucosal pathogens, including the closely related bacterium haemophilus influenzae, utilize iga proteases to aid in defeating the host immune response and facilitate disease but it is unknown whether h. parasuis synthesizes any ...201121737216
haemophilus parasuis serovar 5 nagasaki strain adheres and invades pk-15 cells.haemophilus parasuis is the agent responsible for causing gl+ñsser's disease, which is characterized by fibrinous polyserositis, polyarthritis and meningitis in pigs. the purpose of this study was to investigate the in vitro ability of two h. parasuis serovars of different virulence (serovar 5, nagasaki strain, highly virulent, belonging to serovar 5, and sw114 strain, nonvirulent, belonging to serovar 3) to adhere to and invade porcine kidney epithelial cells (pk-15 line). nagasaki strain was a ...201121839589
Electrotransformation of Haemophilus parasuis with in vitro modified DNA based on a novel shuttle vector.The objective of the present study was to establish a valid transformation method of Haemophilus parasuis, the causative agent of Glässer's disease in pigs, using a novel H. parasuis-Escherichia coli shuttle vector. A 4.2kb endogenous plasmid pYC93 was extracted from an H. parasuis field isolate and completely sequenced. Analysis of pYC93 revealed a region approximately 800bp showing high homology with the defined replication origin oriV of pLS88, a native plasmid identified in Haemophilus ducre ...201121925811
molecular cloning, sequencing, and expression of the outer membrane protein p2 gene of haemophilus parasuis.haemophilus parasuis is the etiological agent of glässer's disease characterized by fibrinous polyserositis, polyarthritis, and meningitis in young pigs. but it is difficult to develop universal serological diagnostic tools and effective vaccines against this disease because of the serovar diversity of the isolates. in this study, enterobacterial repetitive intergenic consensus-polymerase chain reaction, were performed to investigate the gene profile of 111 isolates of h. parasuis from china. an ...201121945800
longitudinal study of respiratory infection patterns of breeding sows in five farrow-to-finish herds.a longitudinal study was carried out in five french farrow-to-finish herds differently affected by respiratory diseases to describe the carrying and infection patterns of batches of sows to various respiratory pathogens during gestation and lactation. an entire batch of sows was followed during two successive reproduction cycles. nasal, tonsillar and oro-pharyngeal swabs and blood samples were taken from each sow 9 and 4 weeks before farrowing and 1 and 4 weeks after farrowing. mycoplasma hyopne ...201120696539
fatal infection due to haemophilus parasuis in a young wild boar (sus scrofa).haemophilus parasuis is a recognized pathogen in domestic pigs; the pathogen has been also isolated from healthy wild boar (sus scrofa). in the current report, a case of fatal h. parasuis infection in a wild boar piglet from central spain is described. the affected animal presented severe pneumonic lesions, inflammation in tarsal joints with presence of fibrinous deposits, and epidural hemorrhage in the atlanto-occipital joint. pure growth of h. parasuis was obtained from lungs and tarsal joints ...201323512924
severity of bovine tuberculosis is associated with co-infection with common pathogens in wild with parasites or viruses drive tuberculosis dynamics in humans, but little is known about their effects in other non-human hosts. this work aims to investigate the relationship between mycobacterium bovis infection and other pathogens in wild boar (sus scrofa), a recognized reservoir of bovine tuberculosis (btb) in mediterranean ecosystems. for this purpose, it has been assessed whether contacts with common concomitant pathogens are associated with the development of severe btb le ...201425350002
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