Publications

TitleAbstractYear
Filter
PMID
Filter
caprine pneumonia iv: pathogenicity of mycoplasma mycoides subspecies capri and caprine strains of mycoplasma mycoides subsp. mycoides for goats. 1976791976
mycoplasma mycoides subspecies mycoides as the cause of a subauricular abscess and mastitis in a goat.a 6-year-old female goat was admitted with right-sided subauricular swelling and facial nerve paralysis. mastitis developed subsequently. the subauricular swelling localized to a mass and was excised. mycoplasma mycoides subsp mycoides was cultured from samples obtained from the mass and mammary gland. after clinical improvement, the goat was discharged to the owner with instructions to isolate the goat and to submit milk samples from the rest of the herd for microbial culturing. mycoplasma spp ...19921429189
genomic maps of some strains within the mycoplasma mycoides cluster.genomic restriction maps for the small colony (sc) strains (pg1, kh3j, gladysdale, and v5) of mycoplasma mycoides subsp. mycoides (the agent of contagious bovine pleuropneumonia) and for mycoplasma strain pg50 (classified as bovine serogroup 7), with respective sizes of 1,280, 1,280, 1,260, 1,230, and 1,040 kbp, were compared with the map (1,200 kbp) for a large colony strain (y goat) of m. mycoides subsp. mycoides. the number and order of all mapped restriction sites were fully conserved in the ...19902254284
[experimental reproduction and the course of contagious bovine pleuropneumonia in a group of cattle and goats: anatomoclinical aspects].five cows were inoculated with 3.10(9) cfu mycoplasma mycoides subsp. mycoides. five other cows and five goats were placed in close contact with inoculated cows. clinical observations, serological survey and microbial excretion by culture for mycoplasma from tracheobronchial washes liquid were carried out during 4 months. mycoplasmas excretion is detected before antibodies responses on all 10 infected cows. the cows never presented clinical respiratory signs and showed no or light lesions but on ...19892485540
the occurrence of infectious diseases in mixed farming of domesticated wild herbivores and livestock in kenya. ii. bacterial diseases.the prevalence of antibodies to brucella spp., mycobacterium paratuberculosis and the mycoplasma spp. causing contagious bovine pleuropneumonia and contagious caprine pleuropneumonia was determined in various species of ruminants on a ranch in the semi-arid zone of southeastern kenya. antibody titers to brucella spp. were found in eland (taurotragus oryx), oryx (oryx beisa) and camels (camelus dromedarius). reactors were not found in buffalo (syncerus caffer), sheep (ovis aries) and goats (capra ...19883373635
comparison of caprine mycoplasmosis caused by mycoplasma capricolum, mycoplasma mycoides subsp. mycoides, and mycoplasma putrefaciens.we reviewed the disease process in goats caused by mycoplasma capricolum, m. mycoides subsp. mycoides, and m. putrefaciens and compared mycoplasma-infected goats (particularly young kids) for microanatomic changes. m. capricolum and m. mycoides subsp. mycoides cause a nearly identical disease: septicemia leading to pyrexia, high morbidity and mortality, interstitial pneumonia, arthritis (including polyarthritis), and mastitis. until recently, m. putrefaciens was known only as a cause of mastitis ...19873667229
mycoplasma mycoides subsp mycoides in the ears of goats associated with an outbreak of systemic mycoplasmosis. 19853833199
caprine mycoplasmosis: widespread infection in goats with mycoplasma mycoides subsp mycoides (large-colony type).a mycoplasma survey of goats with a history of mastitis, polyarthritis, and pneumonia revealed a high incidence of mycoplasma mycoides subsp mycoides (large-colony type). during the early winter kidding season of 1979-1980, kid morbidity in a large commercial dairy herd, evidenced by hyperthermia, polyarthritis, and pneumonia, exceeded 90%. in excess of 200 kids died. colostral cultures of the parent does yielded 157 isolations from 605 goats (26%). additional isolations were made from goats wit ...19836338774
milkborne outbreak of mycoplasma mycoides subspecies mycoides infection in a commercial goat dairy.a commercial goat dairy comprised of about 300 lactating does suddenly began to have a problem with polyarthritis in the kids and clinical mastitis in the does. the problem began 3 to 4 weeks following the introduction of 27 lactating does from a herd with a history of mastitis caused by mycoplasma mycoides subsp mycoides (mmm). over 2 kidding seasons (about 12 months), approximately 170 kids and 70 does died or were euthanatized. affected kids were 2 to 8 weeks old, had multiple warm swollen jo ...19836348004
growth characteristics of large- and small-colony types of mycoplasma mycoides subsp. mycoides on 5% sheep blood agar.mycoplasma mycoides subsp. mycoides of the large-colony (lc) type was isolated in pure culture on 5% sheep blood agar plates inoculated with lung specimens from a 4-month-old toggenburg goat. the growth characteristics of this isolate, of four known lc types, and of five known small-colony (sc) types of m. mycoides subsp. mycoides were compared on 5% sheep blood agar at 2, 5, and 7 days. the sc types were not visible at 2 days and did not grow larger than 0.1 mm, whereas the lc types were visibl ...19836355169
susceptibility of goats and calves after experimental inoculation or contact exposure to a canadian strain of mycoplasma mycoides subsp. mycoides isolated from a goat.transmissibility of mycoplasma mycoides subsp. mycoides infection from experimentally inoculated goats to other goats and calves was studied. eight goats and six calves were housed in an 18 m2 room. six of the goats were inoculated endobronchially with strain d44 isolated from a natural case of polyarthritis in ontario. these six goats died within a week of mycoplasma septicemia. the two contact goats or the six calves never showed signs of disease and m. mycoides subsp. mycoides was not recover ...19836365296
electrophoretic analysis of proteins from mycoplasma capricolum and related serotypes using extracts from intact cells and from minicells containing cloned mycoplasma dna.the acidic proteins of six different mycoplasma serotypes causing bovine or caprine pleuropneumonia were compared by two-dimensional gel electrophoresis of extracts of 35s-labelled cells. the organisms investigated were mycoplasma mycoides subsp. mycoides (pg1), m. mycoides subsp. mycoides (y-goat), m. mycoides subsp. capri (pg3), m. capricolum (california kid), the unclassified bovine serogroup 7 of leach (pg50) and the f38-like group (f38). the results suggested a close relationship between m. ...19846384426
mycoplasma mycoides infections in ruminants: the current situation.recent reports of naturally occurring infections with the large colony variant of mycoplasma mycoides subsp. mycoides are collated and considered together with the results of experimental pathogenicity studies. the information is assessed to indicate where further investigation would add to knowledge of m. mycoides subsp. mycoides as a ruminant pathogen.19846392187
effect of the caprine variant of mycoplasma mycoides subsp mycoides on endothelium, monocytes, and complement of guinea pig, calf, sheep, and goat serum.the caprine variant of mycoplasma mycoides subsp mycoides causes septicemia with coagulopathy in goats. pathogenetic mechanisms that might explain the coagulopathy, the ability of the mycoplasma to persist in the blood, and its specificity for goats were studied. severe endothelial damage was seen by electron microscopy of goat aorta tissue exposed in vitro to 10(7) colony-forming units of mycoplasmas. the mycoplasma did not damage 51cr-labeled adherent cells from peripheral blood of goats. the ...19846395728
pathogenetic mechanisms of mycoplasma mycoides subsp. mycoides septicemia in goats.the variant best known as the large-colony type of m. mycoides subsp. mycoides is responsible for severe outbreaks of septicemia with coagulopathy in goats. our objective was to study aspects of the pathogenesis that might explain the coagulopathy, the persistence of mycoplasmas in the blood of septicemic goats, and the host specificity. the endothelial cells of caprine aorta tissue cultured in vitro and exposed to the m. mycoides underwent severe ultrastructural damage. there was no evidence of ...19846511321
vaccination trials against mycoplasma mycoides subsp. mycoides (large-colony-type) infection in goats.vaccination trials against mycoplasma mycoides subsp. mycoides in suckling and weaned kids are described. a formalin-killed vaccine with adjuvant was used. challenge to the vaccinated suckling kids was by ingestion of dam's infected milk and by subcutaneous inoculation of 10(6) organisms, and to the vaccinated weaned kids, by subcutaneous inoculation alone. the weaned 6-week-old kids were vaccinated, given a booster 3 weeks later and challenged 1 month after that. serological monitoring was by e ...19846511322
role of mycoplasma strain f38 in contagious caprine pleuropneumonia.the role of the f38 group of mycoplasma in contagious caprine pleuropneumonia is assessed with reference to certain criteria whereby a causative relationship between a particular agent and the disease in question is established. the evidence that mycoplasma mycoides subsp. mycoides (large colony) or m. mycoides subsp. capri may be involved in the disease is similarly considered.19846511324
mycoplasma mycoides subsp mycoides, lc type, from goats in italy.a mycoplasma identified as mycoplasma mycoides subsp mycoides lc type was isolated from an outbreak of caprine pleuropneumonia and serofibrinous peritonitis. this isolate was the first to be reported in italy. experimentally, a rapidly fatal condition followed its inoculation into goats and sheep but inoculation of calves did not lead to clinically apparent infection. although the organism was recovered for up to 45 days following experimental inoculation of calves, pathological changes were lim ...19826760527
experimental infection of goats, sheep and calves with the large colony type of mycoplasma mycoides subsp. mycoides.goats, sheep and calves were inoculated intravenously with strain y3343 of the large colony type of mycoplasma mycoides subsp. mycoides isolated from a goat with polyarthritis. the goats and sheep died of septicemia (one was killed in extremis) within eight days. the goats had leukopenia and granulocytopenia. coagulopathy was indicated in some goats; the fibrinogen titer, prothrombin and partial thromboplastin times increased with the progress of disease and the number of platelets decreased dra ...19817008331
observations on mycoplasma mycoides subsp. mycoides infection in saanen goats.an epizootic in white saanen goats, caused by mycoplasma mycoides subsp. mycoides is described. twenty-five flocks totalling approximately 4,500 animals were involved. the disease was characterized by a high, transient temperature, general malaise and mastitis in the lactating does, and a keratoconjunctivitis, arthritis, mycoplasmaemia and death among the kids. in one goat flock there was a precipitous change in the character of the disease, from a predominantly mastitis syndrome to a fulminatin ...19817026494
the ability of mycoplasma mycoides subspecies mycoides and closely related strains from goats and sheep to immunize mice against subspecies capri.small colony (sc) strains of mycoplasma mycoides subsp. mycoides from contagious bovine pleuropneumonia (cbpp) and from goats were compared with large colony (lc) strains of so-called m. mycoides subsp. mycoides from goats and sheep by means of a cross-protection test in which mice were challenged with m. mycoides subsp. capri. of 13 lc strains, all gave partial cross-protection, and 11 were shown to be more closely related than four sc strains to subspecies capri. in a further experiment, six s ...19817026674
identification and characterization of is1296 in mycoplasma mycoides subsp. mycoides sc and presence in related mycoplasmas.is1296, a new insertion sequence belonging to the is3 family of insertion elements has been identified in mycoplasma mycoides subsp. mycoides (mmm) biotype small colony (sc), the agent of contagious bovine pleuropneumonia (cbpp). is1296 is 1485-bp long and has 30-bp inverted repeats. it contains two open reading frames, orfa and orfb, which show significant similarities to the orfs which encode the transposase function of is elements of the is3 family, in particular is150 of escherichia coli. is ...19957628725
mycoplasma infection in a commercial goat dairy caused by mycoplasma agalactiae and mycoplasma mycoides subsp. mycoides (caprine biotype).a commercial dairy goat herd of 600 animals experienced sudden onset of arthritis/polyarthritis, clinical mastitis, and sudden death in does. the offending infectious agents were mycoplasma agalactiae and m. mycoides subsp. mycoides (caprine biotype). the disease syndrome began approximately 4 weeks following the 1) introduction into the herd of a lactating doe with no apparent clinical signs and 2) a breakdown of proper hygienic conditions in the milking parlor. over a period of 3 weeks, 90 doe ...19947858021
insertion element is1296 in mycoplasma mycoides subsp. mycoides small colony identifies a european clonal line distinct from african and australian strains.strains of mycoplasma mycoides subsp. mycoides small colony (sc) type, the agent of contagious bovine pleuropneumonia (cbpp), were analysed with respect to the polymorphism of distribution of a newly discovered insertion element, is1296, on the chromosome. analysis of 64 strains isolated from europe, africa and australia, including four vaccine strains and the type strain pg1, revealed ten different is patterns, forming two main clusters. the european strains originated from outbreaks of cbpp in ...19958574413
high mortality in goats associated with the isolation of a strain of mycoplasma mycoides subsp. mycoides (large colony type).a goat pleuropneumonia outbreak occurring in a herd of 800 animals in the province of ciudad real, spain, is described in this paper. severe respiratory signs and high mortality were the most significant clinical observations. the adult goats presented mainly respiratory symptoms and/or mastitis, whereas the young animals died showing arthritis and/or keratoconjunctivitis. the most significant lesions were found in the thoracic cavity. a focal extensive fibrinonecrotic pleuropneumonia was macros ...19958594844
a pathological and immunohistochemical study of caprine pleuropneumonia induced by subspecies of mycoplasma mycoides.in a population of 700 goats, 150 died; of these, 29 were necropsied. ten of the 29 goats had pleuropneumonia. mycoplasma mycoides subsp. mycoides (large colony) (mmmlc), mycoplasma mycoides subsp. capri (mmc), and pasteurella multocida were isolated from five of the pleuropneumonic goats. gross and microscopical lesions were typical of caprine pleuropneumonia (cpp), with bronchopneumonia, fibrinopurulent or fibrinonecrotic pleuropneumonia and dilatation of the interlobular septa and pleura. imm ...19968814532
characterization of the gene for an immunodominant 72 kda lipoprotein of mycoplasma mycoides subsp. mycoides small colony type.with the aim of characterizing specific immunogenic proteins of mycoplasma mycoides subsp. mycoides small colony (sc) type, the aetiological agent of contagious bovine pleuropneumonia, a gene encoding a major immunogenic protein of 72 kda named p72 was cloned and expressed in escherichia coli. the expressed protein was of the same apparent molecular mass as that produced by the parent strain. the predicted molecular mass of p72, based on the dna-deduced amino acid sequence, was 61.118 kda, signi ...19969004514
polymerase chain reaction and restriction endonuclease digestion for selected members of the "mycoplasma mycoides cluster" and mycoplasma putrefaciens.a specific diagnostic method using the polymerase chain reaction, together with restriction endonuclease digestion patterns, was developed for members of the "mycoplasma mycoides cluster" that normally occur in the united states (i.e., mycoplasma mycoides subsp. mycoides large colony and mycoplasma capricolum subsp. capricolum in addition to "cluster" mycoplasma, bovine serogroup 7, and mycoplasma putrefaciens. the digestion of "cluster" polymerase chain reaction dna (1,225 bp) amplification pro ...19979211239
a pathological and immunohistochemical study of goat kids undergoing septicaemic disease caused by mycoplasma capricolum subsp. capricolum, mycoplasma mycoides subsp. capri and mycoplasma mycoides subsp. mycoides (large colony type).this paper studies the pathological and immunohistochemical findings in 12 kids experimentally infected with mycoplasma capricolum subsp. capricolum (mcc), m. mycoides subsp. capri (mmc) and m. mycoides subsp. mycoides (large colony type), (mmmlc). for the demonstration of mcc, mmc and mmmlc antigens an immunoperoxidase technique based on the labelled streptavidin biotin method was used in the 12 kids inoculated with mycoplasmas and the three control kids inoculated with mycoplasma medium. all 1 ...19989588108
genetic and serological analysis of the immunogenic 67-kda lipoprotein of mycoplasma sp. bovine group 7.the gene encoding a lipoprotein of 67 kda, named p67, was cloned from mycoplasma sp. bovine group 7 strain pg50 and expressed in escherichia coli k12. analysis of the amino acid sequence derived from the dna sequence of the p67 gene revealed a typical prokaryotic signal peptidase ii membrane lipoprotein lipid attachment site and a transmembrane structure domain in the leader sequence at the amino-terminal end of the protein. protein p67 showed 91% identical amino acid residues to the lipoprotein ...19989766210
antigen heterogeneity among mycoplasma mycoides subsp. mycoides sc isolates: discrimination of major surface proteins.the protein and antigen profiles of 60 isolates, strains and the type strain pg1 of mycoplasma mycoides subsp. mycoides s.c. were compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblot analysis. analysis using contagious bovine pleuropneumonia antisera and hyperimmune rabbit sera against several representative strains revealed some differences in protein profiles and variability in antigens among strains from different geographic regions. the most common antigenic ...19989810618
genetic and serological analysis of lipoprotein lppa in mycoplasma mycoides subsp. mycoides lc and mycoplasma mycoides subsp. capri.the genes encoding the 62-kda lipoproteins from the mycoplasma mycoides subsp. mycoides large-colony type (lc) strain y-goat and the m. mycoides subsp. capri strain pg3 were cloned and analyzed by sequencing. these two lipoproteins have been named lppa[mmymylc] and lppa[mmyca], and their corresponding genes have been named lppa[mmymylc] and lppa[mmyca], respectively. the nucleotide and deduced amino acid sequences of these two lipoproteins showed a very high degree of similarity between these tw ...199910066658
genomic and antigenic differences between the european and african/australian clusters of mycoplasma mycoides subsp. mycoides sc.mycoplasma mycoides subsp. mycoides small-colony type (sc), the aetiological agent of contagious bovine pleuropneumonia (cbpp), can be grouped into two major, epidemiologically distinct, clusters. one cluster contains strains isolated from different european countries since 1980 and a second cluster contains african and australian strains collected over the last 50 years. genetic analysis of representative strains from the two clusters revealed a genomic segment of 8.84 kb, located close to a co ...200010708386
phylogeny of the mycoplasma mycoides cluster as shown by sequencing of a putative membrane protein gene.the mycoplasma mycoides cluster is made of six species that are closely related both genetically and phenotypically. two are of particular importance, m. mycoides subsp. mycoides sc causing contagious bovine pleuropneumonia and m. capricolum subsp. capripneumoniae causing contagious caprine pleuropneumonia. the sequences of a putative membrane protein gene and partial flanking open reading frames have been obtained from various strains in this cluster, including all reference strains. sequence a ...200010727835
mycoplasmas isolated from the respiratory tract of cattle and goats in tanzania.a microbiological study of the mycoplasma flora in the respiratory tracts of cattle and goats in selected regions of tanzania is described. in the examination of cattle, mycoplasmas were isolated from 60 (17.8%) of the 338 examined lung samples, 8 (47.1%) of the 17 lymph nodes, 4 (13.3%) of the 30 pleural fluid samples and 4 (3.9%) of the 103 nasal swabs examined. all the isolates were identified as mycoplasma mycoides subsp. mycoides, small colony type except for one isolate from pleural fluid ...200011126579
demonstration of mycoplasma capricolum subsp. capripneumoniae and mycoplasma mycoides subsp. mycoides, small colony type in outbreaks of caprine pleuropneumonia in eastern tanzania.an outbreak of caprine pleuropneumonia involving about 1200 goats in the coast and morogoro regions of eastern tanzania is reported. the major clinical findings were severe respiratory distress, fever, mucopurulent nasal discharge and high mortality involving all age groups and both sexes of goats. the morbidity and mortality rates were 45%-90% and 14%-50%, respectively. the principal pathological lesions were confined to the thoracic cavity and comprised hydrothorax and serofibrinous pleuropneu ...200011126580
caprine mycoplasmal mastitis in nigeria.in a study to investigate the current status of intramammary mycoplasmosis in caprine udders in nigeria, a total of 57 and 24 milk samples were collected from udders of goats affected by mastitis and from apparently normal goats' udders, respectively. acute and chronic mastitis were more commonly observed in goats between 1 and 3 years old. mycoplasma agalactiae and mycoplasma capricolum occurred at a significantly higher rate (p<0.05) in udders affected by mastitis than in normal healthy udders ...200111163719
disease caused by mycoplasma mycoides subspecies mycoides lc in hungarian goat herds.the occurrence of a goat disease caused by mycoplasma mycoides subsp. mycoides lc in hungary is reported. the disease occurred in two goat herds in the spring of 1999. in one herd 25% of the 4-12 weeks old kids (10 animals) while in the other herd 33% of the 6-12 weeks old kids (20 animals) became affected. the goat kids developed polyarthritis. the most severe lesions developed in the carpal joints. all animals died after 3-8 days of disease. four dead kids were necropsied. all of them had sero ...200011402711
molecular analysis of field strains of mycoplasma capricolum subspecies capripneumoniae and mycoplasma mycoides subspecies mycoides, small colony type isolated from goats in tanzania.a molecular analysis of strains of mycoplasma capricolum subsp. capripneumoniae (m. capripneumoniae) and mycoplasma mycoides subsp. mycoides, small colony type (m. mycoides sc) isolated from goats was performed using the amplified fragment length polymorphism (aflp) and pulsed-field gel electrophoresis (pfge) fingerprinting techniques. among the 11 field strains of m. capripneumoniae from tanzanian goats, two aflp patterns were demonstrated, with 10 of the strains showing indistinguishable patte ...200111423192
rapid detection of contagious bovine pleuropneumonia by a mycoplasma mycoides subsp. mycoides sc capsular polysaccharide-specific antigen detection latex agglutination test.a latex agglutination test (lat) has been developed for the diagnosis of contagious bovine pleuropneumonia (cbpp). the latex microspheres were coated with mmmsc polyclonal immunoglobulin g antiserum and detected mmmsc antigen in the serum of cattle infected with cbpp and in growth medium containing mmmsc. the specific antigen recognized by this test appeared to be the capsular polysaccharide (cps). the lat recognized all 23 strains of mmmsc examined in this study, with a sensitivity level of 2 n ...200312626448
inactivation of mycoplasma species involved in contagious agalactia.the suitability of 5 agents for the inactivation of different field strains of the four mycoplasma species associated with contagious agalactia syndrome in goats, i.e. mycoplasma agalactiae, mycoplasma mycoides subsp. mycoides lc, mycoplasma capricolum subsp. capricolum and mycoplasma putrefaciens, was investigated. immunoprophylaxis of this syndrome is still based on inactivated vaccines, which traditionally use formalin as the inactivating agent. moreover, the limited information existing abou ...200414964115
isolation and characterisation of an indian strain of mycoplasma mycoides subsp. mycoides type lc from a case of caprine arthritis.isolation and characterisation of an indian strain of mycoplasma mycoides subsp. mycoides lc from a case of caprine arthritis is reported in the study. the isolate was identified based on biochemical, digitonin sensitivity and growth inhibition tests. the virulence of the organism was studied by pathogenicity test in mice and goat. the antigenic and genomic profile of the isolate was compared with that of the standard strain (y-goat). using different sets of primers, polymerase chain reaction wa ...200415178001
variability of a glucose phosphotransferase system permease in mycoplasma mycoides subsp. mycoides small colony.intraclonal antigenic variation in pathogenic mycoplasma species is considered an important feature of host-pathogen interaction. such intraclonal protein variation was observed for the interaction of mycoplasma mycoides subsp. mycoides small colony, the agent of contagious bovine pleuropneumonia, with mab 3f3. colony immunostaining allows the definition of 3f3 on- and 3f3 off-type variants, which revert at low frequency. targets of mab 3f3 were shown to be surface located, and resided on multip ...200415583154
isolation of mycoplasma mycoides subsp. mycoides (lc variant, y-goat) from naturally aborted bovine fetuses.seven strains of mycoplasma mycoides subsp. mycoides (lc, y-goat) were isolated from 4 of 85 (4.7%) cases of bovine abortion. the biochemical characters of all the isolates were homogenous (except for one sucrose negative isolate), but variation in sensitivity to neomycin, kanamycin and streptomycin were noted. the only gross lesions of internal organs in aborted fetuses were congestion of liver and lungs, and hemorrhagic patches on the heart surface. significant microscopic lesions were encount ...198916726715
isolation of mycoplasma mycoides, mycoides (lc variant), from two naturally aborted caprine fetuses.described in this study are 2 cases of caprine abortion associated with the lc type of mycoplasma mycoides , subsp. mycoides . this mycoplasma and mycoplasma mycoides , subsp. capri had been previously reported in adult goats in this herd. the abortion took place in the latter part of gestation. samples from heart blood, lung and pleural exudate were collected for the isolation of mycoplasmas and other bacterials in both fetuses. two strains of mycoplasma mycoides , subsp. mycoides (lc variant) ...199516727795
mycoplasma mycoides subsp. capri and mycoplasma mycoides subsp. mycoides lc can be grouped into a single subspecies.mycoplasma mycoides subsp. capri and mycoplasma mycoides subsp. mycoides lc can be combined into one taxon on the basis of several contributions on both dna sequence and protein analyses reported in the literature. moreover, for the differentiation and identification of mycoplasmas of the "mycoides cluster", we investigated the rpob gene, encoding the beta-subunit of the rna polymerase. a segment of 527 bp of the rpob gene was amplified from 31 strains of ruminant mycoplasmas by pcr. the nucleot ...200616973118
field trial of a combined vaccine against caprine contagious agalactia: humoral immune response in lactating goats.two vaccines against mycoplasma agalactiae and mycoplasma mycoides subsp. mycoides (large colony type) were developed using inactivated strains selected in previous characterization studies. formaldehyde and phenol were used as the inactivating agents for vaccines a and b, respectively. aluminium hydroxide plus purified saponin (quil-a) were added to both vaccines as adjuvant. the field trial was designed to evaluate the specific humoral immune response to the two mycoplasma species in lactating ...200717158077
field trial of two dual vaccines against mycoplasma agalactiae and mycoplasma mycoides subsp. mycoides (large colony type) in goats.two vaccines against mycoplasma agalactiae and mycoplasma mycoides subsp. mycoides (lc type) were developed using inactivated strains selected in previous characterization studies. the vaccines differed in terms of the adjuvants used: aluminium hydroxide (vaccine a) or aluminium hydroxide plus purified saponin (vaccine b). these vaccines were tested on 60 pregnant goats and 60 seronegative kids that were challenged by placing in a herd with a history of caprine contagious agalactia (cca). our fi ...200717239497
the in vitro effect of six antimicrobials against mycoplasma putrefaciens, mycoplasma mycoides subsp. mycoides lc and mycoplasma capricolum subsp. capricolum isolated from sheep and goats in jordan.respiratory disease in sheep and goats is a major problem in jordan and is often associated with mycoplasma species. without effective vaccines, control is mainly by chemotherapy, but the uncontrolled use of antimicrobials has led to concerns about the potential development of antimicrobial resistance. the in vitro effect of chloramphenicol, florfenicol, enrofloxacin, tylosin, erythromycin and oxytetracycline was determined against 32 isolates of mycoplasma species-m. mycoides subsp. mycoides lc ...200617405622
a pcr for the detection of mycoplasmas belonging to the mycoplasma mycoides cluster: application to the diagnosis of contagious agalactia.contagious agalactia is a mycoplasmal infection caused by mycoplasma agalactiae, mycoplasma mycoides subsp. mycoides lc, m. mycoides subsp. capri, mycoplasma capricolum subsp. capricolum and mycoplasma putrefaciens. identification of the causative organisms is usually performed by isolation and classical biochemical and serological tests, though this is a lengthy and cumbersome process for mycoplasmas. specific pcr assays have been developed for the identification of mycoplasma agalactiae and m. ...200717606362
strains of mycoplasma mycoides subsp. mycoides small colony type isolated in china between 1953 and 1960 show close similarity to strains of the africa/australia cluster.in this study, six chinese strains of mycoplasma mycoides subsp. mycoides small colony type (mmmsc) isolated between 1953-1960 were analysed and their molecular characteristics compared to those of the african pg1 and afade strains, the european c305 and 138/5 strains and the closely related caprine m. mycoides subsp.mycoides large colony type y-goat strain. pcr amplification of long dna fragments showed that the six chinese strains, the pg1 strain and the y-goat strain, just like afade, did not ...200917936044
in vitro antimycoplasmal activity of six jordanian medicinal plants against three mycoplasma species.the in vitro effect of six jordanian traditional medicine plant methanolic extracts were tested against 32 isolates of mycoplasma species; mycoplasma mycoides subsp. mycoides lc (6), mycoplasma capricolum subsp. capricolum (8) and m. putrefaciens (18), all isolated from either nasal swabs or milk, from sheep and goats in different regions in jordan. all mycoplasma species showed susceptibility to artemisia herba-alba and artemisia arborescens with mic ranges from 3.125-12.5 mg/ml. allium sativum ...200717969714
detection of mycoplasmas in goat milk by flow cytometry.the detection of mycoplasma in milk can be performed by either culture techniques or polymerase chain reaction (pcr) based methods. although pcr can reduce the average diagnostic time to 5 h in comparison with the several days for the isolation of the agent, there is still a need to develop methods, which could give earlier results. for this purpose, we tested the ability of flow cytometry (fc) to detect mycoplasmas in milk samples. milk samples inoculated with four different mycoplasmas, mycopl ...200717972304
effects on goat milk quality of the presence of mycoplasma spp. in herds without symptoms of contagious agalactia.this study was designed to assess the possible effects of mycoplasmas on the quality of milk produced by goat herds in a contagious agalactia (ca) endemic area with absence of classical symptoms. several factors related to milk quality (percentages of fat, total protein, lactose and total solids, standard plate counts (spc) and presence of staphylococcus aureus) were compared in mycoplasma-infected and non-infected herds. to define the ca status of 26 herds on the island of lanzarote (spain), wh ...200918922196
mycoplasma leachii sp. nov. as a new species designation for mycoplasma sp. bovine group 7 of leach, and reclassification of mycoplasma mycoides subsp. mycoides lc as a serovar of mycoplasma mycoides subsp. capri.the mycoplasma mycoides cluster consists of six pathogenic mycoplasmas causing disease in ruminants, which share many genotypic and phenotypic traits. the m. mycoides cluster comprises five recognized taxa: mycoplasma mycoides subsp. mycoides small colony (mmmsc), m. mycoides subsp. mycoides large colony (mmmlc), m. mycoides subsp. capri (mmc), mycoplasma capricolum subsp. capricolum (mcc) and m. capricolum subsp. capripneumoniae (mccp). the group of strains known as mycoplasma sp. bovine group ...200919502315
extended surveillance for cbpp in a free country: challenges and solutions regarding the potential caprine reservoir.contagious bovine pleuropneumonia (cbpp) is a severe respiratory disease of cattle and buffalo caused by mycoplasma mycoides subsp. mycoides "small colony" (mmmsc). the agent of cbpp has been isolated from goats in different countries including cbpp-free areas. goats can therefore be regarded as a putative mmmsc reservoir. no diagnostic test for cbpp surveillance in goats has been proposed as yet. furthermore, serological tests could be seriously hampered by a widespread caprine infection due to ...201121620492
kinetics of substrate oxidation and hydrogen peroxide production by mycoplasma mycoides subsp. mycoides large colony (lc) type and mycoplasma mycoides subsp. capri.mycoplasma mycoides subsp. mycoides large colony (lc) type is a pathogen of goats causing contagious agalactia and respiratory disease, found on all continents where small ruminants are kept. it shares close genetic characteristics with m. mycoides subsp. capri. substrate oxidation by 22 strains of m. mycoides subsp. mycoides lc from nine countries was compared with that of eight strains of m. mycoides subsp. capri from five countries. there was considerable similarity in the substrates used, bu ...200919457526
experimental infection of goats with an unusual strain of mycoplasma mycoides subsp. capri isolated in jordan.goats were infected experimentally with a mycoplasma (the "irbid" strain) isolated previously from a goat with contagious agalactia in northern jordan. the strain was unusual in that, although it had been identified by molecular methods as mycoplasma mycoides subsp. mycoides lc/mycoplasma mycoides subsp. capri, it showed no inhibition of growth by any of the hyperimmune rabbit antisera conventionally used to speciate members of the mycoplasma mycoides cluster. animals were infected either intrat ...200919446837
mycoplasma mycoides subsp. mycoides biotype large colony isolates from healthy and diseased goats: prevalence and typing.most severe goat mycoplasmosis outbreaks in france are caused by mycoplasma mycoides subsp. mycoides biotype lc (mmmlc). however, mmmlc can also be recovered from ear canals of healthy goats or from bulk milk collected in herds showing no clinical signs of mycoplasmosis. to improve our understanding of how mmmlc strains are balanced between pathogenic ones and asymptomatically carried ones, descriptive epidemiological data were analysed, together with the genomic fingerprints of isolates generat ...200717224248
Displaying items 1 - 59 of 59