[use of deletion mutants of plasmid rp4::d3112 for the genetic analysis of pseudomonas aeruginosa bacteriophage d3112].the hybrid plasmid rp4::d3112 becomes unstable in escherichia coli k-12 cells under certain growth conditions. the deletion mutants of this plasmid are formed at a high frequency. all the deletions selected have a specific feature: they start in the left end, at the point of joining of plasmid and phage dna, and remove different portions of the phage genome. the deletion mutants have been used for genetic mapping of d3112. we have localized the repressor gene ci (0-1.3 kb), 3 early genes (1.3-14 ...19836418616
complementation analysis of the aliphatic amidase genes of pseudomonas aeruginosa.a plasmid, pcl34, capable of autonomous replication in escherichia coli and pseudomonas aeruginosa has been constructed which carries the promoter and structural gene (amie) for p. aeruginosa amidase, but not the regulator gene (amir). plasmid pcl34 has been mobilized from e. coli to p. aeruginosa using the broad host range plasmid rp4. complementation studies were performed in p. aeruginosa strains carrying various amidase mutations. measurements of amidase activity in the recipients under indu ...19846440948
[the effect of certain escherichia coli genes on the appearance of the tcs phenotype, conferred by plasmid rp4 with an integrated genome of the d3112 pseudomonas aeruginosa phage].the possibility of the sos system activation caused by introduction of a hybrid plasmid rp4::d3112 (where d3112 is a genome of the transposable phage of pseudomonas aeruginosa) into escherichia coli was examined. it has been shown previously that the presence of this plasmid confers to e. coli a so called tcs phenotype: e. coli (rp4::d3112) forms normal colonies and grows at 42 degrees c but does not divide and becomes filamentous at 30 degrees c, probably because of e. coli dna damages generate ...19938405972
metabolic deprivation: a lead to containment in bacterial releases.experiments were carried out in non-sterile soil, with or without several substrates (sucrose, citrate, lactose) as energy source, and with or without bacterial inoculates able or unable to utilize these substrates. the bacteria were enterobacter agglomerans, escherichia coli, pseudomonas aeruginosa or pseudomonas aureofaciens; they either did or did not contain plasmid rp4. it was found that sucrose is degraded by the indigenous microflora and/or free soil enzymes to give extracellular glucose ...19947921354
[oxidation of n-alkanes by pseudomonas aeruginosa strain carrying the plasmid pbs251].pseudomonas aeruginosa pao8 cannot use n-alkanes or their respective alcohols as a sole carbon source. however, it can grow on n-alkanes when plasmid pbs251 is transferred into its cells. the hybrid plasmid pbs251 is a plasmid rp4 containing genes which control the capability to grow on n-alkanes of the c6-c12 series. studies of n-alkane oxidation by p. aeruginosa pao8 carrying pbs251 have shown that this plasmid controls the inducible alkane and alcohol oxidizing activities; the subsequent step ...19853937960
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