tn5-mediated integration of the delta-endotoxin gene from bacillus thuringiensis into the chromosome of root-colonizing pseudomonads.gene replacement mediated by tn5 sequences was used to integrate the bacillus thuringiensis subsp. kurstaki hd-1 delta-endotoxin gene (tox) into the chromosome of two corn root-colonizing strains of pseudomonas fluorescens. a tn5 transposase deletion element containing the tox gene (delta tn5-tox) was substituted for a tn5 element previously present in the p. fluorescens chromosome. two classes of delta tn5-tox elements were made. the first class encodes kanamycin resistance in addition to the t ...19863023294
integration of the delta-endotoxin gene of bacillus thuringiensis into the chromosome of root-colonizing strains of pseudomonads using tn5.the delta-endotoxin gene (tox) from bacillus thuringiensis subsp. kurstaki hd-1 was cloned into tn5 and the resulting tn5-tox element transposed from a vector plasmid into the chromosome of six corn-root-colonizing strains of pseudomonas fluorescens and agrobacterium radiobacter. chromosomal integration of the tox gene maximized stability and minimized the potential for horizontal transfer of the tox gene to other bacterial species. expression of the tox gene was demonstrated by western blot ana ...19863026918
taxonomy of phytopathogenic pseudomonads.phytopathogenic pseudomonads were placed into four major groups on the basis of nutritional and physiological characteristics. group i consists of 86 strains of phytopathogens distinguishable from other fluorescent pseudomonads by low growth rates, ability to induce hypersensitivity on tobacco, absence of arginine dihydrolase, and relatively limited ranges of carbon sources. most of these strains cannot utilize benzoate, 2-ketogluconate, spermine, beta-alanine, l-isoleucine, l-valine, and l-lysi ...19705411761
rerouting the plant phenylpropanoid pathway by expression of a novel bacterial enoyl-coa hydratase/lyase enzyme function.the gene for a bacterial enoyl-coa hydratase (crotonase) homolog (hchl) previously shown to convert 4-coumaroyl-coa, caffeoyl-coa, and feruloyl-coa to the corresponding hydroxybenzaldehydes in vitro provided an opportunity to subvert the plant phenylpropanoid pathway and channel carbon flux through 4-hydroxybenzaldehyde and the important flavor compound 4-hydroxy-3-methoxybenzaldehyde (vanillin). expression of the pseudomonas fluorescens an103 hchl gene in two generations of tobacco plants cause ...200111449058
identification and deletion analysis of the promoter of the pepper sar8.2 gene activated by bacterial infection and abiotic stresses.the pepper sar8.2 gene, casar82a, was locally and systemically induced in pepper plants which had been infected by xanthomonas campestris pv. vesicatoria or by pseudomonas fluorescens. the dna 1,283 bp sequence upstream of the casar82a gene was assessed with regard to the activity of the casar82a promoter fused to the beta-glucuronidase (gus) reporter gene, via an agrobacterium-mediated transient expression assay. in tobacco leaves which transiently expressed the -831 bp casar82a promoter, gus a ...200616395580
pseudomonads associated with midrib rot and soft rot of butterhead lettuce and endive.during the past ten years, bacterial soft rot and midrib rot of glasshouse-grown butterhead lettuce (lactuca sativa l. var. capitata) and field-grown endive (cichorium endivia l.) has become increasingly common in the region of flanders, belgium. severe losses and reduced market quality caused by bacterial rot represent an important economical threat for the production sector. symptoms of midrib rot are a brownish rot along the midrib of one or more inner leaves, often accompanied by soft rot of ...200516637164
metabolic diversion of the phenylpropanoid pathway causes cell wall and morphological changes in transgenic tobacco stems.studies involving transgenic plants with modifications in the lignin pathway reported to date, have received a relatively preliminary characterisation in relation to the impact on vascular integrity, biomechanical properties of tissues and carbon allocation to phenolic pools. therefore, in this study transgenic tobacco plants (nicotiana tabacum cv xhfd 8) expressing various levels of a bacterial 4-hydroxycinnamoyl-coa hydratase/lyase (hchl) gene have been characterised for cell wall and related ...200717120022
pseudomonas syringae lytic transglycosylases coregulated with the type iii secretion system contribute to the translocation of effector proteins into plant cells.pseudomonas syringae translocates virulence effector proteins into plant cells via a type iii secretion system (t3ss) encoded by hrp (for hypersensitive response and pathogenicity) genes. three genes coregulated with the hrp t3ss system in p. syringae pv. tomato dc3000 have predicted lytic transglycosylase domains: pspto1378 (here designated hrph), pspto2678 (hopp1), and pspto852 (hopaj1). hrph is located between hrpr and avre1 in the hrp pathogenicity island and is carried in the functional clu ...200717827286
salicylic acid biosynthetic genes expressed in pseudomonas fluorescens strain p3 improve the induction of systemic resistance in tobacco against tobacco necrosis virus.abstract application of salicylic acid induces systemic acquired resistance in tobacco. pcha and pchb, which encode for the biosynthesis of salicylic acid in pseudomonas aeruginosa, were cloned into two expression vectors, and these constructs were introduced into two root-colonizing strains of p. fluorescens. introduction of pchba into strain p3, which does not produce salicylic acid, rendered this strain capable of salicylic acid production in vitro and significantly improved its ability to in ...199818944940
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