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[biological safety investigations of the production of human insulin by genetically-engineered e. coli k-12 cells. 3. plasmid transfer by natural transformation and transduction].the transfer of the expression plasmid of human insulin psw3 by natural transformation and by transduction with phage p1 was investigated in laboratory media and in surface water samples. whereas pseudomonas stutzeri could be transformed by shuttle vectors which could replicate in this microorganism, no transformants were found with the insulin expression vector. e. coli k-12 could be transduced by phage p1vir only with chromosomal markers, not with plasmid dna. as the production strain w3110iqm ...19938338615
meningitis due to pseudomonas stutzeri in a patient infected with human immunodeficiency virus. 19968852995
study of common aerobic flora of human cerumen.cerumen is the product of the secretion of the sebaceous, ceruminous or apocrine glands together with cells exfoliated from the cornified stratum of the epithelium of the external auditory canal (eac). in the present study we identified and quantified common flora of human cerumen. the mean count obtained was 10(6) microorganisms per ml of cerumen suspension. in 24 pools of cerumen (33.3 per cent) the isolates were monomicrobial, staphylococcus epidermidis (12), corynebacterium spp (10), staphyl ...19989775288
the isolation, purification and biological activity of a novel antibacterial compound produced by pseudomonas stutzeri.a novel compound designated zafrin [4beta-methyl-5, 6, 7, 8 tetrahydro-1 (4beta-h)-phenanthrenone] was isolated from a crude extract of a marine bacterium identified as pseudomonas stutzeri. zafrin showed strong antibacterial activity against both gram-positive and gram-negative bacteria. the compound was purified and its structure was elucidated by spectroscopic methods including 1h-nuclear magnetic resonance (nmr), 13c-nmr, 1d-nmr and 2d-nmr spectroscopy. it could be demonstrated that a purifi ...200818093138
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