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reduced formation of granulomata in gamma(delta) t cell knockout balb/c mice inoculated with mycobacterium avium subsp. paratuberculosis.the role of gamma(delta) t cells in the bovine immune response to mycobacterium avium subsp. paratuberculosis (m. paratuberculosis) infection is poorly understood. accordingly, using balb/c mice that are innately susceptible to m. paratuberculosis, we compared wild-type and gamma(delta) t cell knockout balb/c mice to study the protective roles of gamma(delta) t cells in m. paratuberculosis infection. ten-week-old mice were inoculated intraperitoneally with either a low dose (4 x 10(6) colony-for ...200011055864
temporal mycobacterium paratuberculosis infection in t-cell receptor (tcr)-alpha and tcr-delta-deficient mice.mycobacterium paratuberculosis is the causative agent of paratuberculosis (johne's disease), a chronic inflammation of the terminal portion of the ileum in ruminants. the predominance of cell-mediated immunity in early stages of the disease suggests that t lymphocytes are essential to protect the host from infection with m. paratuberculosis. in this study, we investigated the role of alphabeta and gammadelta t cells in resistance to m. paratuberculosis infection using a t-cell receptor (tcr) kno ...200212383644
cellular immune responses to 35 kda recombinant antigen of mycobacterium avium paratuberculosis.mycobacterium avium paratuberculosis is the causative agent of johne disease, a chronic ulcerative intestinal condition in ruminant animals. owing to the predominance of cellular response in subclinical forms of the infection, identification of m. a. paratuberculosis antigens eliciting host cell-mediated immune (cmi) reaction is crucial for early control of the disease. a 35 kda protein of m. a. paratuberculosis was studied for its ability to elicit cmi responses using a mouse model. lymphoproli ...200616502104
isolation of mycobacterium avium subspecies paratuberculosis from non-ruminant wildlife living in the sheds and on the pastures of greek sheep and goats.this study aimed to: (1) investigate whether non-ruminant wildlife interfacing with dairy sheep and goats of four greek flocks endemically infected with mycobacterium avium subspecies paratuberculosis (map) harboured map and (2) genetically compare the strains isolated from the wildlife to those isolated from the small ruminants of these flocks. we cultured and screened, by polymerase chain reaction (pcr), pooled-tissue samples from 327 wild animals of 11 species for the map-specific is900 inser ...200817578601
genetic resistance of mice to mycobacterium paratuberculosis is influenced by slc11a1 at the early but not at the late stage of infection.we have recently described the development of a luminescent mycobacterium paratuberculosis strain of bovine origin expressing the luxab genes of vibrio harveyi. with this luminescent isolate, fastidious and costly enumeration of cfu by plating them on agar can be replaced by easy and rapid luminometry. here, we have reevaluated the effect of slc11a1 (formerly nramp1) polymorphism on susceptibility to m. paratuberculosis, using this luminometric method. a series of inbred mouse strains were infec ...200818285491
immunogenicity and protective efficacy of the mycobacterium avium subsp. paratuberculosis attenuated mutants against challenge in a mouse model.johne's disease (jd), caused by mycobacterium avium subsp. paratuberculosis (map), results in serious economic losses worldwide especially in cattle, sheep and goats. to control the impact of jd on the animal industry, an effective vaccine with minimal adverse effects is urgently required. in order to develop an effective vaccine, we used allelic exchange to construct three mutant map strains, leud, mpt64 and seca2. the mutants were attenuated in a murine model and induced cytokine responses in ...201122107851
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