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[evaluation of the usefulness of the agglutination test with mangifera indica extract for the identification of pathogenic yersinia enterocolitica strains].the study was performed on 137 y. enterocolitica strains belonging to various serological groups, including 75 03 group strains isolated form human clinical material. the agglutination test on slides was carried out on this strains using mangifera indica extract of own production. agglutinating preparation obtained from the seeds of m. indica agglutinated y. enterocolitica organisms possessing the pvy plasmid and crmox+ phenotype in dilutions to 1.56 micrograms/ml. in identification tests conduc ...19979554150
first isolation in puglia of human yersinia enterocolitica: two cases report. 19807459027
hla-b27 does not affect invasion of arthritogenic bacteria into human cells.to investigate the effect of hla-b27 expression on entry of salmonella typhimurium and yersinia enterocolitica into human cells.19989733458
a complex composed of sycn and yscb functions as a specific chaperone for yopn in yersinia pestis.human pathogenic yersinia resist host defences, in part through the expression and delivery of a set of plasmid-encoded virulence proteins termed yops. a number of these yops are exported from the bacteria directly into the cytoplasm of their eukaryotic host's cells upon contact with these cells. the secreted yopn protein (also known as lcre) is required to block yop secretion in the presence of calcium in vitro or before contact with a eukaryotic cell in vivo. in this study, we characterize the ...199810094626
susceptibility to beta-lactam agents of yersinia enterocolitica biotype 4, serotype o3 isolated in various parts of the world.forty-eight human isolates of yersinia enterocolitica of biotype 4, serotype o3 from various parts of the world were examined for susceptibility to 13 beta-lactam agents. the intracellular beta-lactamases of each of the 48 strains were examined. isolates from europe, asia and brazil (phage type viii) or south africa and hungary (phage type ixa) produced both enzyme a and enzyme b, whereas isolates from new zealand and australia (phage type ixb) lacked the cephalosporinase enzyme b. among the sev ...19957608962
temperature-inducible surface fibrillae associated with the virulence plasmid of yersinia enterocolitica and yersinia pseudotuberculosis.when cultivated at 37 degrees c in static broth, human clinical isolates of yersinia enterocolitica (serogroups o:3, o:8, and o:9) and yersinia pseudotuberculosis (serogroup o:iii) produced numerous nonflagellar surface appendages, which appeared as a lawn of fine fibrillae, each having a diameter of 1.5 to 2.0 nm and a length of 50 to 70 nm. cultivation at 22 degrees c resulted in complete disappearance of the fibrillae. the phenotypic expression of these appendages was correlated with the pres ...19853881355
experimental yersinia enterocolitica infection in rodents: a model for human yersiniosis.yersinia enterocolitica infection in humans causes a broad spectrum of diseases ranging from acute bowel disease to extraintestinal manifestations such as reactive arthritis, erythema nodosum and uveitis. during the last decade a fascinating part of the molecular biology of the pathogenicity of human pathogenic yersinia species has been unraveled. pathogenicity factors such as protein tyrosine phosphatase, protein kinase, thrombin- and collagen-binding factors have been identified and characteri ...19938363822
population genetics of human, animal, and environmental yersinia strains.multilocus enzyme electrophoresis was used to analyze 244 strains of nine yersinia species isolated from the environment, animals, and humans at 18 genes encoding metabolic enzymes. all 18 enzymes were polymorphic. among the 137 electrophoretic types (ets) distinguished, the mean allelic diversity per locus was 0.531. yersinia frederiksenii ets were divided into three major clusters that were separated by a large genetic distance, and one et was more closely related to yersinia enterocolitica. t ...19938434911
outer membrane protein yada of enteropathogenic yersiniae mediates specific binding to cellular but not plasma fibronectin.the binding of bacteria or bacterial products to host proteins of tissue extracellular matrix may be a mechanism of tissue adherence. we investigated interactions of the plasmid-encoded outer membrane protein yada, which confers pathogenic functions on enteropathogenic yersiniae, with fibronectin. attachment of yada-positive and yada-negative recombinant yersinia enterocolitica strains to cartilage-derived human cellular fibronectin and human plasma fibronectin in the solid phase revealed that y ...19938500887
expression of mhc class ii molecules on human monocytes is regulated independently from each other after phagocytosis of bacteria.reactive arthritis is usually self-limiting polyarthritis which develops in hla-b27 positive individuals after certain gastrointestinal or urogenital infections. the pathogenesis of reactive arthritis is unknown but t cells seem to have a crucial role. most of the antigen-specific t cells isolated from the synovial fluid have been mhc class ii restricted. the role of antigen presentation in the pathogenesis of reactive arthritis has been studied relatively little. in this work the authors studie ...19968560195
penetration of m cells and destruction of peyer's patches by yersinia enterocolitica: an ultrastructural and histological study.yersinia enterocolitica is enteropathogenic for man and rodents. previous studies provided evidence that y. enterocolitica invades the lymphoid follicles of the peyer's patches (pp) of the small intestine. in this study y. enterocolitica-induced tissue alterations of the follicle-associated epithelium (fae) and the underlying pp tissue were analysed by scanning (sem) and transmission electron microscopy (tem) as well as by conventional histological examination. for this purpose, an experimental ...19968606357
survey for psychrotrophic bacterial pathogens in minimally processed lettuce.a total of 120 minimally processed, cut and packaged lettuce samples were purchased from retail supermarkets or provided by a salad production facility over an 8-month period. the samples were tested for total aerobic plate counts and for the presence of potentially pathogenic species belonging to the genera of listeria, aeromonas and yersinia. the aerobic plate counts ranged from 103 to 109 colony forming units (cfu) g-1. most samples (76%) contained between 105 and 107 cfu g-1 total aerobic ba ...200010849276
in-vitro antimicrobial susceptibility of yersinia enterocolitica isolates from stools of patients in the netherlands from 1982-1991.the mics of 24 antimicrobial agents were determined for 335 strains of yersinia enterocolitica isolated from faeces in the netherlands during 1982-1991. the isolates belonged to biotypes 1a, 1b, 2, 3, 4 and to serotypes o3, o5.27, o6.3, o7.8, o8, o9. almost all strains were susceptible to piperacillin, piperacillin/tazobactam, imipenem, all cephalosporins except cefazolin, the aminoglycosides, quinolones, co-trimoxazole, doxycycline and chloramphenicol but resistant to amoxycillin, co-amoxiclav ...19958626266
differential secretion of interleukin-8 by human epithelial cell lines upon entry of virulent or nonvirulent yersinia enterocolitica.epithelial cells of the intestinal mucosa are among the first cells encountered by invasive pathogens. bacterial invasion of the mucosa gives rise to an inflammatory response, characterized by the influx of polymorphonuclear leukocytes. the chemotactic stimulus responsible for this accumulation is unknown, but several in vitro studies have demonstrated that epithelial cells secrete the chemokine interleukin-8 (il-8), a potent chemoattractant of polymorphonuclear leukocytes, upon bacterial entry. ...19968675314
yersinia-hsp60-reactive t cells are efficiently stimulated by peptides of 12 and 13 amino acid residues in a mhc class ii (i-ab)-restricted manner.heat shock proteins (hsp) are immunodominant antigens in microbial infections. previous work from this laboratory demonstrated that yersinia-hsp60 (y-hsp60)-reactive cd4+ alpha beta t cells play an important role for resolution of y. enterocolitica infections in mice. in the present study we identified two epitopes of y-hsp60 recognized by cd4+ th1 cell clones. the epitopes comprise 12 (214-225) and 13 (74-86) amino acid (aa) residues of y-hsp60, and are the first described for mhc class ii (i-a ...19968706327
note: isolation, characterization and epidemiology of yersinia enterocolitica from humans and animals.during an 11-year period (1983 to 1994), 51 strains of yersinia enterocolitica were isolated from humans and animals. specimens were collected from a total of 3601 sources consisting of 956 patients with enteritis, 300 patients with urinary tract infection, 1564 healthy humans, 510 swine, 38 guinea-pigs, 118 rats and 115 rabbits. five strains of y. enterocolitica, bio/serogroups 2/o:9 and 4/o:3, virulence positive, were recovered from patients. forty-two variants of y. enterocolitica belonging t ...19968972095
the influence of hla b27 and interferon-gamma on the invasion and persistence of yersinia in primary human fibroblasts.to examine the role of the human hla class i molecule b27 in the direct interaction of yersinia and human cells we performed experimental infections of constitutive hla b27-positive and -negative human fibroblast monolayers with yersinia enterocolitica o.3. the numbers of yersiniae invading hla b27-positive cells were within the range of hla b27-negative cells. treatment of fibroblasts with interferon-gamma (ifn-gamma) to enhance hla b27 expression did not alter invasion. ifn-gamma reduced titer ...19969007822
[isolation and characterization of yersinia enterocolitica from pigs and bovines in chile].the occurrence of yersinia enterocolitica in tonsils and rectal swabs from 100 healthy pigs and the rectal swabs of 100 healthy cattle slaughtered at santiago-chile were analysed. yersinia enterocolitica was isolated from 48 (48%) pigs but not from cattle. 98.2% of strains were of 4/o3 bioserogroup, considered to be pathogenic for humans. all of the strains were resistant to penicillin producing beta-lactamase. most of them were resistant to neomicin and tetracycline. the pyv marker was used to ...19979312970
emerging foodborne diseases: an evolving public health challenge.the epidemiology of foodborne disease is changing. new pathogens have emerged, and some have spread worldwide. many, including salmonella, escherichia coli o157:h7, campylobacter, and yersinia enterocolitica, have reservoirs in healthy food animals, from which they spread to an increasing variety of foods. these pathogens cause millions of cases of sporadic illness and chronic complications, as well as large and challenging outbreaks over many states and nations. improved surveillance that combi ...19979366593
interaction of yersinia enterocolitica and y. pseudotuberculosis with platelets.yersinia enterocolitica is a bacterium capable of growth at 4 degrees c in donated blood and has been responsible for many deaths following transfusion. interaction of y. enterocolitica with blood cells is of interest in understanding the mechanisms of survival and growth in blood. the closely related organism y. pseudotuberculosis is known to invade platelets and cause platelet aggregation by a mechanism that involves expression of the chromosomal inv gene. yersinia isolates were made to expres ...200111761186
a role for rad23 proteins in 26s proteasome-dependent protein degradation?treatment of cells with genotoxic agents affects protein degradation in both positive and negative ways. exposure of s. cerevisiae to the alkylating agent mms resulted in activation of genes that are involved in ubiquitin- and 26s proteasome-dependent protein degradation. this process partially overlaps with the activation of the er-associated protein degradation pathway. the dna repair protein rad23p and its mammalian homologues have been shown to inhibit degradation of specific substrates in r ...200211804604
an analysis of human pathogens found in horse/mule manure along the john muir trail in kings canyon and sequoia and yosemite national parks.to determine the prevalence of microorganisms that are potentially pathogenic for humans in horse/mule manure along the john muir trail (jmt).200212092962
single-tube, nested pcr for the diagnosis of human brucellosis in kuwait.the polymerase chain reaction (pcr) offers a sensitive and specific way of detecting microbial dna in clinical samples. the aims of the present study were to develop an assay, based on a single-tube, nested pcr, for identifying brucella in samples of human blood and then to explore the use of this test in diagnosis. the primers chosen were derived from is711, the insertion sequence gene found in all species of brucella. the assay amplified a 52-bp final product which was detected colorimetricall ...200212171621
yersinia enterocolitica-induced interleukin-8 secretion by human intestinal epithelial cells depends on cell differentiation.in response to bacterial entry epithelial cells up-regulate expression and secretion of various proinflammatory cytokines, including interleukin-8 (il-8). we studied yersinia enterocolitica o:8-induced il-8 secretion by intestinal epithelial cells as a function of cell differentiation. for this purpose, human t84 intestinal epithelial cells were grown on permeable supports, which led to the formation of tight monolayers of polarized intestinal epithelial cells. to analyze il-8 secretion as a fun ...19989488416
[yersinia enterocolitica iha-test (author's transl)].612 serum specimens were examined for antibodies against yersinia enterocolitica (y.e.) with the widal technique, and an indirect (passive) hemagglutination test. the widal test was carried out with antigens of y.e. serovar 0:3 and 0:9. human erythrocytes coated with antigens of both serovarieties were used for the y.e.-iha-test. this test system showed higher agglutinin titres than the widal reaction. both methods were equivalent concerning their specificity. the y.e. iha-test is suitable for s ...19817275163
functional expression of adhesive peptides as fusions to escherichia coli flagellin.an expression system for studying epitopes of adhesion proteins based on fusion of gene fragments into flic(h7) of escherichia coli is described. we constructed the system by an in-frame insertion of dna fragments encoding one, two or three of the fibronectin-binding d repeats present in the fibronectin-binding protein a (fnbpa) of staphylococcus aureus, into the flic(h7) gene region encoding the variable domain of the h7 flagellin. the constructs were expressed by in trans complementation in th ...19979514121
surveillance of human yersinia enterocolitica infections in belgium: 1967-1996.between 1967 and 1996, > 18,700 strains of yersinia species, excluding yersinia pestis, were recovered in belgium from a variety of gastrointestinal and extraintestinal sites in patients. full identification and serotyping were performed by the two belgian reference laboratories. yersinia enterocolitica serogroup o:3 predominated (79.4% of strains), followed by serogroup o:9 (11.1%). the remaining 9.5% of isolates belonged to serogroups and related species generally considered nonpathogenic. acu ...19989675452
genomic fingerprinting of yersinia enterocolitica species by degenerate oligonucleotide-primed polymerase chain reaction.a novel version of the polymerase chain reaction (pcr) termed degenerate oligonucleotide-primed pcr (dop-pcr) was used to fingerprint the bacterial genome of yersinia enterocolitica species. eight well-characterized reference strains of y. enterocolitica (ribotype, serotype, biotype and zymotype) were examined. optimal experimental conditions for reproducibility, sensitivity and specificity were obtained using a single dop-primer. all the strains gave distinct dop-pcr profiles composed of 12 to ...19947925230
bacterial infection of human hematopoietic stem cells induces monocytic differentiation.differentiation of hematopoietic stem cells (hscs) can be influenced by different stimuli, including cytotoxic agents, certain cytokines, and contact with pathogens. infection may result in dysregulation of these important progenitor cells and therefore interfere with the availability of blood cells. in this study we analyzed the effect of bacterial infection on hscs concerning surface marker expression and cytokine release. listeria monocytogenes and yersinia enterocolitica accelerated maturati ...200414987733
the probiotic escherichia coli strain nissle 1917 interferes with invasion of human intestinal epithelial cells by different enteroinvasive bacterial pathogens.the probiotic escherichia coli strain nissle 1917 (mutaflor) of serotype o6:k5:h1 was reported to protect gnotobiotic piglets from infection with salmonella enterica serovar typhimurium. an important virulence property of salmonella is invasion of host epithelial cells. therefore, we tested for interference of e. coli strain nissle 1917 with salmonella invasion of int407 cells. simultaneous administration of e. coli strain nissle 1917 and salmonella resulted in up to 70% reduction of salmonella ...200415039098
typing of yersinia enterocolitica isolates by its profiling, rep- and eric-pcr.thirty-five yersinia enterocolitica strains isolated from humans, pigs and foxes were analysed by genotyping including intergenic transcribed sequence (its) profiling, rep- and eric-pcr. eric-pcr revealed the presence of seven different genotypes. amplification of the 16s-23s rdna spacer region by its profiling gave similar results with nine different genotypes. rep-pcr was found to be more discriminatory for typing of y. enterocolitica than eric-pcr and its profiling. fifteen different dna patt ...200415330984
[detection of salmonella, listeria spp., vibrio spp., and yersinia enterocolitica in frozen seafood and comparison with enumeration for faecal indicators: implication for public health].infections transmitted through consumption of contaminated seafood is a significant source of human morbidity. the aim of this study was to compare the detection of salmonella, listeria, vibrio, and yersinia enterocolitica in frozen seafood with results from enumeration of conventional faecal indicators. a total of 213 crustaceans or molluscs were purchased from local vendors in italy: 74% were harvested in italy, 25% from other european countries and 1% from outside europe. listeria spp. was is ...200415366511
duration of carriage and transmission of yersinia enterocolitica biotype 4, serotype 0:3 in dogs.human infections with pathogenic strains of yersinia enterocolitica have been linked to contact with dogs excreting these microorganisms. this study examines the carriage and transmission of y. enterocolitica biotype 4, serotype 03 in dogs. fourteen 6-month-old cross-bred dogs were separated into 5 groups, 2 containing 4 dogs (i and ii) and the others 2 dogs (iii-v). each of the 4 dogs in group i and 2 of the dogs in group ii were inoculated orally with the test strain. bacteriological examinati ...19947995357
behavior of yersinia enterocolitica strains inoculated in model cheese treated with high hydrostatic pressure.the effects of high hydrostatic pressure treatment and the ability for survival, repair, and growth of three human pathogenic serotypes (o:1, o:3, o:8) of yersinia enterocolitica were investigated in washed-curd model cheese made with pasteurized bovine milk. samples were treated at 300, 400, and 500 mpa for 10 min at 20 degrees c and analyzed at 0, 1, 7, and 15 days to assess the viability of the yersinia population. a long-term study (up to 60 days of ripening after high hydrostatic pressure t ...200515771177
electron microscopy and immunolabeling of yersinia antigens in human synovial fluid cells.recent findings have emphasized the involvement of bacterial antigens in synovitis in reactive arthritis. it is still unclear, however, in what form the microbial material exists in the joint. both antigen-containing cells and intact bacteria have been proposed as candidates on the basis of immunohistochemical studies of the synovial membrane. this study addresses that question by electron microscopy and peroxidase immunolabeling of synovial fluid cells from three patients with reactive arthriti ...19948070157
comparison of siderophore production and utilization in pathogenic and environmental isolates of yersinia enterocolitica.yersinia enterocolitica strains of serotypes lethal to mice have been reported previously to produce an endogenous siderophore. in this study, an ethyl acetate-extractable siderophore was characterized and given the name yersiniophore. yersiniophore was produced by 16 of 16 human isolates of serotypes o:4, o:4,32, o:8, o:21, and one nonhuman isolate of serotype o:21. it was not produced by isolates of serotype o:3, o:5, or o:9. one strain of yersinia pseudotuberculosis produced yersiniophore, bu ...19948126201
yada mediates specific binding of enteropathogenic yersinia enterocolitica to human intestinal submucosa.the binding of live yersinia enterocolitica to frozen sections of human intestine was investigated qualitatively by monitoring the binding of bacteria by using gram or immunoperoxidase staining as well as quantitatively by a new enzyme immunoassay-on-slide method. we have demonstrated that the binding of various y. enterocolitica serotypes and escherichia coli clones to frozen sections of human intestine is mediated by the yersinia adhesin, yada. the yada-mediated binding occurs mainly at the su ...19948132332
identification of a protein-tyrosine phosphatase from human platelet membranes by an immobilon-based solid phase assay.the present investigations show that a 53-kda platelet-membrane protein is a protein tyrosine phosphatase. identification involved a novel methodology in which membrane proteins are resolved by polyacrylamide gel electrophoresis and then transblotted to a polyvinylidene difluoride membrane surface-labeled with [32p-tyr](glu4.tyr1)n. phosphatase activity appears as clear areas in the autoradiograph of the renatured 32p western blot. studies using this new solid-phase system indicate that a 53-kda ...19938238898
use of heme-protein complexes by the yersinia enterocolitica hemr receptor: histidine residues are essential for receptor function.the abilities of two bacterial active heme transporters, hmbr of neisseria meningitidis and hemr of yersinia enterocolitica, to use different heme sources were compared. while hmbr-expressing cells used only hemoglobin (hb) and heme, hemr-expressing bacteria were able to grow on hb, heme, myoglobin, hemopexin, catalase, human and bovine serum albumin-heme, and haptoglobin-hemoglobin complexes as sources of iron. expression of functional hemr allowed escherichia coli cells to respond to heme-cont ...199910498719
a mage-a4 peptide presented by hla-a2 is recognized by cytolytic t lymphocytes.the mage-encoded antigens that are recognized by cytolytic t lymphocytes (ctl) are shared by many tumors and are strictly tumor specific. clinical trials involving therapeutic vaccination of cancer patients with mage antigenic peptides or proteins are in progress. to increase the range of patients eligible for therapy with peptides, it is important to identify additional mage epitopes. we have used a method to identify ctl epitopes, which selects naturally processed peptides. cd8(+) t cells, obt ...199910540345
a case-control study of yersinia enterocolitica infections in auckland.to identify major risk factors for yersinia enterocolitica (ye) and identify measures to reduce ye infections.199910575769
yersinia enterocolitica: overview and epidemiologic correlates.yersinia enterocolitica comprises both pathogenic and nonpathogenic members. distinguished by biogrouping, serogrouping, and ecological distribution, commonly occurring pathogenic serobiogroups, e.g., o:3/4; o:5,27/2; o:8/1b; o:9/2, possess both chromosomal and plasmid-mediated virulence traits. studies have revealed several (oral, blood transfusion) modes of acquisition, elucidated the putative role of chromosomal and plasmid-encoded virulence factors in the pathogenesis of human infection, and ...199910602666
host recognition and target differentiation by factor h, a regulator of the alternative pathway of complement.factor h is responsible for recognition of host cells and tissues and mediates discrimination among microbial pathogens during activation of the alternative pathway of complement (ap). its unique structure of 20 scr domains arranged in a flexible chain permits a variety of functional sites to interact with complement proteins and surface markers in a biological example of single-molecule combinatorial chemistry. in addition to the complement regulatory site located in the n-terminal four scr dom ...200010904114
infection of human enterocyte-like cells with rotavirus enhances invasiveness of yersinia enterocolitica and y. pseudotuberculosis.mixed infection with rotavirus and either yersinia enterocolitica or y. pseudotuberculosis was analysed in caco-2 cells, an enterocyte-like cell line highly susceptible to these pathogens. results showed an increase of bacterial adhesion and internalisation in rotavirus-infected cells. increased internalisation was also seen with escherichia coli strain hb101 (pri203), harbouring the inv gene from y. pseudotuberculosis, which is involved in the invasion process of host cells. in contrast, the su ...200011023186
structure and sequence analysis of yersinia yada and moraxella uspas reveal a novel class of adhesins.the non-fimbrial adhesins, yada of enteropathogenic yersinia species, and uspa1 and uspa2 of moraxella catarrhalis, are established pathogenicity factors. in electron micrographs, both surface proteins appear as distinct 'lollipop'-shaped structures forming a novel type of surface projection on the outer membranes. these structures, amino acid sequence analysis of these molecules and yada gene manipulation suggest a tripartite organization: an n-terminal oval head domain is followed by a putativ ...200011080146
yersinia enterocolitica infection with multiple liver abscesses uncovering a primary hemochromatosis.a 60-year-old man, although treated with antibiotics, suffered from a severe pyrexial illness of unknown origin, weight loss and intermittent abdominal pain. there was no history of diarrhea or common infections. computed tomography and ultrasound imaging showed uncharacteristic multiple small lesions of the entire liver parenchyma. these lesions were histologically pyogenic abscesses. in addition, an unexpected, pronounced accumulation of iron pigment in hepatocytes and second degree fibrotic c ...200111252417
preferential recognition of a microbial metabolite by human vgamma2vdelta2 t cells.human vgamma2vdelta2 t cells are stimulated by prenyl pyrophosphates, such as isopentenyl pyrophosphate (ipp), and play important roles in mediating immunity against microbial pathogens and have potent anti-tumor activity. (e)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (hmbpp) has been identified as a metabolite in the 2-c-methyl-d-erythritol-4 phosphate (mep) pathway for isoprenoid biosynthesis that is used by many bacteria and protozoan parasites. we find that hmbpp is the major vgamma2vdelta ...200717446209
antibodies against listerial protein 60 act as an opsonin for phagocytosis of listeria monocytogenes by human dendritic cells.human-monocyte-derived dendritic cells (modc) are very efficient in the uptake of listeria monocytogenes, a gram-positive bacterium which is an important pathogen in humans and animals causing systemic infections with symptoms such as septicemia and meningitis. in this work, we analyzed the influence of blood plasma on the internalization of l. monocytogenes into human modc and compared the uptake of l. monocytogenes with that of salmonella enterica serovar typhimurium and yersinia enterocolitic ...200111292729
yope of yersinia, a gap for rho gtpases, selectively modulates rac-dependent actin structures in endothelial cells.yersinia spp. inject effector proteins (yersinia outer proteins, yops) into target cells via a type iii secretion apparatus. the effector yope was recently shown to possess gap activity towards the rho gtpases rhoa, rac and cdc42 in vitro. to investigate the intracellular, 'in vivo' targets of yope we generated a yersinia enterocolitica strain [wa(pylcr+e)] that injects 'life-like' amounts of yope as only effector. primary human umbilical vein endothelial cells (huvec) were infected with wa(pylc ...200111298653
[yersinia enterolitica infections: 2. impact on human health].the clinical picture of yersiniosis in humans and its prevalence in the human population is described in detail. mass production of animals, development of meat factories based on sophisticated chains of cold storage units and international trade of meat products and animals are believed to be the reasons for the increasing prevalence of yersiniosis in humans. in germany, anti-yersinia antibodies are found in up to 40% of the average population. the financial losses for the national economy cann ...200111314588
yersinia enterocolitica serum resistance proteins yada and ail bind the complement regulator c4b-binding protein.many pathogens are equipped with factors providing resistance against the bactericidal action of complement. yersinia enterocolitica, a gram-negative enteric pathogen with invasive properties, efficiently resists the deleterious action of human complement. the major y. enterocolitica serum resistance determinants include outer membrane proteins yada and ail. lipopolysaccharide (lps) o-antigen (o-ag) and outer core (oc) do not contribute directly to complement resistance. the aim of this study wa ...200818769718
a regulatory network controls expression of the in vivo-expressed hrep protease of yersinia enterocolitica.the human enteropathogen yersinia enterocolitica survives and replicates in the lymphoid tissues of its host. previous in vivo analyses of gene expression revealed that various chromosomal genes are expressed at this stage of infection, but not in vitro. one of these, termed hrep, encodes a protease that is necessary for full virulence of y. enterocolitica. using transposon mutagenesis, we identified three genes, pypa, pypb, and pypc, as positive regulators of hrep transcription. pypa is an inne ...200919114497
[modulating effect of serotonin on the development of human leukocytes apoptosis induced by yersinia].effect of biogenic amine serotonin on the development of human blood leukocytes during interaction with species from yersinia genus (y. pestis ev, y. pseudotuberculosis serovars i and iv, y. enterocolitica serovars 09 and 03) was studied in model system in vitro using flow cytofluorometry. serotonin in concentration 10(-5) m had differently marked effects on yersinia spp.-induced apoptosis of leukocytes. pattern of the observed changes depended from species and serovar of yersinia. serotonin inh ...200819186545
serum concentration of hakata antigen, a member of the ficolins, is linked with inhibition of aerococcus viridans growth.hakata antigen (hakata) is a novel serum glycoprotein that consists of collagen- and fibrinogen-like domains, similar to ficolin/p35. our research suggested that serum hakata may be a target of a polysaccharide (psa) produced by aerococcus viridans.200212367778
pathogenic yersinia enterocolitica strains increase the outer membrane permeability in response to environmental stimuli by modulating lipopolysaccharide fluidity and lipid a structure.pathogenic biotypes of yersinia enterocolitica (serotypes o:3, o:8, o:9, and o:13), but not environmental biotypes (serotypes o:5, o:6, o:7,8, and o:7,8,13,19), increased their permeability to hydrophobic probes when they were grown at ph 5.5 or in egta-supplemented (ca(2+)-restricted) media at 37 degrees c. a similar observation was also made when representative strains of serotypes o:8 and o:5 were tested after brief contact with human monocytes. the increase in permeability was independent of ...200312654821
survivability and long-term preservation of bacteria in water and in phosphate-buffered saline.to evaluate the suitability of using sterile water and phosphate-buffered saline (pbs) for preservation of bacteria pathogenic to plants or humans.200312803555
enzyme-linked immunosorbent assay to differentiate the antibody responses of animals infected with brucella species from those of animals infected with yersinia enterocolitica o9.enzyme-linked immunosorbent assays using antigens extracted from brucella abortus with n-lauroylsarcosine differentiated natural brucella-infected animals from brucella-vaccinated or yersinia enterocolitica o9-infected animals. a field trial in mongolia showed cattle, sheep, goat, reindeer, camel, and human sera without infection could be distinguished from brucella-infected animals by conventional serological tests.200312853409
yersinia enterocolitica o:3 isolated from patients with or without reactive arthritis induces polyclonal activation of b cells and autoantibody production in vivo.the mechanisms by which arthritis-provoking pathogens such as yersinia enterocolitica interact with the human immune system to produce inflammatory synovitis are not well known. one of the immunomodulating mechanisms used against these pathogens is the polyclonal activation of lymphocytes. in this study, we investigated the extent of the b-lymphocyte activation induced in mice by a strain of y. enterocolitica o:3 (fcf 526) isolated from a patient with arthritis, and compared it with two other st ...200314567555
effect of cytokines on invasion and survival of yersinia in primary human fibroblasts.bacteria or bacterial antigen triggering reactive arthritis have been detected in inflamed joint tissue and fluid of patients. although live yersiniae have not yet been found in joints of patients with yersinia arthritis, early dissemination and propagation has been proposed. in this study, we investigated the influence of the proinflammatory cytokines interleukin- (il-) 1beta, tumor necrosis factor-(tnf-) alpha, the th1 lymphokine interferon- (ifn-) gamma, and the th2 lymphokine il-4 on the int ...199910206147
antilisterial activity of a broad-spectrum bacteriocin, enterocin lr/6 from enterococcus faecium lr/6.enterocin lr/6, a purified bacteriocin, exhibited broad inhibitory spectrum both against related as well as some food-borne pathogens such as listeria monocytogenes, yersinia enterocolitica, aeromonas sp., shigella sp., and bacillus licheniformis. in this investigation, we have focused on l. monocytogenes as the target organism, as it is not only an important pathogen but can also survive over a wide range of environmental conditions such as refrigeration temperature, low ph, and high-salt conce ...201019957209
genetic and biochemical characterization of an exopolygalacturonase and a pectate lyase from yersinia enterocolitica.yersinia enterocolitica, an invasive foodborne human pathogen, degrades polypectate by producing two depolymerizing enzymes, pectate lyase (pl) and polygalacturonase (pg). the gene encoding the pg activity, designated pehy, was located in a 3-kb genomic fragment of y. enterocolitica atcc 49397. the complete nucleotide sequence of this 3-kb fragment was determined and an open reading frame consisting of 1803 bp was predicted to encode a pg protein with an estimated m(r) of 66 kda and pi of 6.3. t ...199910446714
antibacterial activity of artemisia nilagirica leaf extracts against clinical and phytopathogenic bacteria.the six organic solvent extracts of artemisia nilagirica were screened for the potential antimicrobial activity against phytopathogens and clinically important standard reference bacterial strains.201020109237
common aspects of human and primate seronegative arthritis.a 27-year-old female lowland gorilla developed an asymmetric oligoarthritis 3 months post-partum. there was no evidence of an antecedent gastrointestinal or genitourinary infection. serum was negative for rheumatoid factor and antinuclear antibody. synovial fluid revealed 2000 white blood cells with negative cultures and polarized microscopy. studies on synoviocytes were the following: (1) facs analysis revealed surface expression of a b27-like epitope of the cells. (2) analysis of intracellular ...200415517649
flavoridin inhibits yersinia enterocolitica uptake into fibronectin-adherent hela cells.in this study, three structurally distinct disintegrins (flavoridin, echistatin, kistrin) were used as molecular probes to further characterize the molecular mechanisms underlying yersinia enterocolitica infection of host cells. the activity of the three disintegrins on y. enterocolitica uptake into fibronectin-adherent hela cells was evaluated at disintegrin doses which were non-cytotoxic and unable to induce cell detachment. flavoridin resulted to be the most effective in inhibiting bacterial ...200515927747
survival and growth of yersinia enterocolitica strains inoculated in skimmed milk treated with high hydrostatic pressure.four human pathogenic strains of yersinia enterocolitica (serotypes o:1, o:3, o:8, and o:9) were inoculated (7-8 log cfu/ml) in uht skimmed milk and treated at 300, 400, and 500 mpa for 10 min at 20 degrees c, and then kept at 8 degrees c to assess their evolution for 15 days. treatments at 400 and 500 mpa caused the highest lethality, generally reaching counts below detection level (1 cfu/ml) in the culture media. at 300 mpa, the most baroresistant serotypes were o:3 and o:8. after 15 days of s ...200516014300
adhesion, invasion, and agglutination mediated by two trimeric autotransporters in the human uropathogen proteus mirabilis.fimbriae of the human uropathogen proteus mirabilis are the only characterized surface proteins that contribute to its virulence by mediating adhesion and invasion of the uroepithelia. pmi2122 (aipa) and pmi2575 (taap) are annotated in the genome of strain hi4320 as trimeric autotransporters with "adhesin-like" and "agglutinating adhesin-like" properties, respectively. the c-terminal 62 amino acids (aa) in aipa and 76 aa in taap are homologous to the translocator domains of yada from yersinia en ...201020805336
identification of burkholderia mallei and burkholderia pseudomallei adhesins for human respiratory epithelial cells.burkholderia pseudomallei and burkholderia mallei cause the diseases melioidosis and glanders, respectively. a well-studied aspect of pathogenesis by these closely-related bacteria is their ability to invade and multiply within eukaryotic cells. in contrast, the means by which b. pseudomallei and b. mallei adhere to cells are poorly defined. the purpose of this study was to identify adherence factors expressed by these organisms.201020920184
rapid multiple-locus variable-number tandem-repeats analysis of shigella spp. using multicolour capillary electrophoresis.the multiple-locus variable-number tandem-repeats analysis (mlva) method for genotyping has proven to be a fast and reliable typing tool in several bacterial species. mlva is in our laboratory the routine typing method for salmonella enterica subsp. enterica serovar typhimurium, escherichia coli (two assays), listeria monocytogenes and yersinia enterocolitica. the gram-negative invasive enteropathogenic bacterium shigella is the most common cause of bacillary dysentery (shigellosis) worldwide, a ...201020951171
induction of cytokine synthesis by flagella from gram-negative bacteria may be dependent on the activation or differentiation state of human monocytes.we have previously demonstrated that salmonellae, but not escherichia coli or yersinia enterocolitica, stimulates tumor necrosis factor alpha (tnfalpha) production in the human promonocytic cell line u38. subsequent analysis revealed that the tnfalpha-inducing activity of salmonellae was associated with flagellin, a major component of flagella from gram-negative bacteria. in the present study, we have explored the basis for the apparent specificity of action of salmonella flagella on tnfalpha ex ...199910496893
yersinia enterocolitica bacteremia in a chronic, mildly iron-overloaded dialysis patient (february).objective: to report an unusual case of yersinia enterocolitica in a chronic, mildly iron-overloaded dialysis patient lacking other typical risk factors for bacteremia, who was treated successfully with a third-generation cephalosporin as monotherapy for a short duration of treatment. case summary: a 76-year-old dialysis-dependent man developed y. enterocolitica bacteremia after 8 days of hospitalization. one month prior to presentation, his ferritin level was mildly elevated at 571.5 ng/ml, whi ...201121266629
application of fluorescent amplified fragment length polymorphism for comparison of human and animal isolates of yersinia enterocolitica.an amplified fragment length polymorphism (aflp) method, developed to genotype yersinia enterocolitica, has been used to investigate 70 representative strains isolated from humans, pigs, sheep, and cattle in the united kingdom. aflp primarily distinguished y. enterocolitica strains according to their biotype, with strains dividing into two distinct clusters: cluster a, comprising largely the putatively pathogenic biotypes (bt2 to -4), and cluster b, comprising the putatively nonpathogenic biotyp ...200516151073
a hypervariable n-terminal region of yersinia lcrv determines toll-like receptor 2-mediated il-10 induction and mouse virulence.the virulence antigen lcrv of yersinia enterocolitica o:8 induces il-10 in macrophages via toll-like receptor 2 (tlr2). the tlr2-active region of lcrv is localized within its n-terminal amino acids (aa) 31-57. sequencing of codons 25-92 of the lcrv gene from 59 strains of the three pathogenic yersinia species revealed a hypervariable hotspot within aa 40-61. according to these sequence differences, seven lcrv groups were identified, with y. pestis and y. pseudotuberculosis represented in group i ...200516239347
phenotypic and transcriptional analysis of the osmotic regulator ompr in yersinia pestis.the osmotic regulator ompr in escherichia coli regulates differentially the expression of major porin proteins ompf and ompc. in yersinia enterocolitica and y. pseudotuberculosis, ompr is required for both virulence and survival within macrophages. however, the phenotypic and regulatory roles of ompr in y. pestis are not yet fully understood.201121345178
Yersinia enterocolitica: Pathogenesis, virulence and antimicrobial resistance.Yersinia enterocolitica is a heterogeneous group of strains, which are classified into 6 biogroups, and into more than 57 O serogroups. However, the human pathogenic strains most frequently isolated worldwide belong to serogroups O:3, O:5,27, O:8 and O:9. The major route of Y. enterocolitica infection is through contaminated foods or water. The primary pathogenic event is colonization of the intestinal tract where most of the pathologic effects and clinical manifestations occur. Temperature and ...201122019131
[evaluation of the yersinia enterocolitica pathogenicity through some phenotypic and genotypic characters: crmox agar positivity and presence of the ail gene].sixty-nine strains of y. enterocolitica isolated from environmental and human matrices (waste water, food and faeces) were studied in order to evidence the presence of ail gene, calcium-dependency and congo red absorption for pathogenic strains identification. out of 24 clinical strains, the ail gene was present in 21 (87%), among which 79% were crmox-positive as well. on the contrary, none of the 45 environmental strains showed the ail gene although only one (isolated from cooked vegetables) wa ...200516523709
the serodiagnosis of human infections with yersinia enterocolitica and yersinia pseudotuberculosis.the techniques of sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and immunoblotting were evaluated for the serodiagnosis of human infections with yersinia enterocolitica and yersinia pseudotuberculosis. lipopolysaccharide (lps) was prepared from strains comprising four serogroups of y. enterocolitica and five serogroups of y. pseudotuberculosis, tested against 200 sera submitted to the laboratory of enteric pathogens for routine serodiagnosis, and shown to contain antibodie ...200616872375
escape from immune surveillance by capnocytophaga canimorsus.capnocytophaga canimorsus, a commensal bacterium from dogs' mouths, can cause septicemia or meningitis in humans through bites or scratches. here, we describe and characterize the inflammatory response of human and mouse macrophages on c. canimorsus infection. macrophages infected with 10 different strains failed to release tumor necrosis factor (tnf)- alpha and interleukin (il)-1 alpha . macrophages infected with live and heat-killed (hk) c. canimorsus 5 (cc5), a strain isolated from a patient ...200717205476
yersiniosis in zoo marmosets (callitrix jacchuss) caused by yersinia enterocolitica 4/o:3.the aim of this research was to describe two fatal cases of yersinia enterocolitica bioserotype 4/o:3 infection in non-human primates and to characterise the isolates by pcr and pfge. in july 2004, two marmosets (callitrix jacchuss) born in captivity in zagreb zoo, died following a few days of intermittent diarrhoea in intervals of 2 weeks. the pathomorphological diagnosis of the female (born in 1997) and the male (born in 1995) marmoset, was disseminated miliary necrosis of the liver. y. entero ...200717223285
collagenous duodeno-ileo-colitis with transient igg deficiency preceded by yersinia enterocolitica intestinal infection: case report and review of literature.a case-report of a man with chronic diarrhoea is presented. after an unsuccessful treatment of an intestinal yersioniosis, the diagnosis of collagenous intestinal disease affecting duodenum, ileum and colon was made. in addition, a igg transient deficiency was observed. the literature about gastrointestinal involvement, concomintant infection by yersinia and igg deficiency in collagenous colitis is reviewed.200717408550
prevalence of pathogenic yersinia enterocolitica in pigs slaughtered at a swiss abattoir.human yersiniosis is the third most common enteric disease after campylobacteriosis and salmonellosis in many european countries. however, epidemiological data on the prevalence of pathogenic yersinia enterocolitica in animals and humans is insufficient. pigs are assumed to be the main reservoir of pathogenic y. enterocolitica because pig is so far the only animal species from which pathogenic strains have frequently been isolated. this work was conducted to study the frequency of ail-positive y ...200717727997
occurrence of campylobacter, salmonella, yersinia enterocolitica and listeria monocytogenes in some retail food products in novi sad.the official reporting system in the province of vojvodina (pv) indicates that cases of human salmonellosis were partly covered by complete epidemiological investigation including laboratory analysis of the suspected food. intestinal campylobacteriosis and yersiniosis and four cases of septicemias caused by listeria monocytogenes were not fully epidemiologically investigated. actual country legislation on food safety does not include provisions for a routine control of the above mentioned pathog ...200718251233
the importance of the rcs phosphorelay in the survival and pathogenesis of the enteropathogenic yersiniae.the human-pathogenic yersiniae represent an ideal species group to study the evolution of highly virulent bacteria, with yersinia pestis having emerged from the enteropathogen y. pseudotuberculosis an estimated 20 000 years ago. sequence data reveal that the y. pestis genome is in the early stages of decay and contains hundreds of non-functioning pseudogenes, some of which may be important in the enteric lifestyle of y. pseudotuberculosis. bioinformatic analysis of pseudogenes from seven y. pest ...200818375804
hada is an atypical new multifunctional trimeric coiled-coil adhesin of haemophilus influenzae biogroup aegyptius, which promotes entry into host cells.the oca (oligomeric coiled-coil adhesin) family is a subgroup of the bacterial trimeric autotransporter adhesins, which includes structurally related proteins, such as yada of yersinia enterocolitica and nada of neisseria meningitidis. in this study, we searched in silico for novel members of this family in bacterial genomes and identified hada (haemophilus adhesin a), a trimeric autotransporter expressed only by haemophilus influenzae biogroup aegyptius causing brazilian purpuric fever (bpf), a ...200919290916
insights into the evolution of sialic acid catabolism among bacteria.sialic acids comprise a family of nine-carbon amino sugars that are prevalent in mucus rich environments. sialic acids from the human host are used by a number of pathogens as an energy source. here we explore the evolution of the genes involved in the catabolism of sialic acid.200919470179
controlled activation of the cpx system is essential for growth of yersinia enterocolitica.the cpx two-component signal transduction system regulates the expression of genes in response to stimuli associated with the maintenance of the bacterial cell envelope. additionally, the cpx system is important for virulence in several bacterial pathogens. in this study, we analyzed the cpx system of the human enteropathogen yersinia enterocolitica. we provide evidence that transcription of cpxr is autoregulated and that the response regulator cpxr negatively affects transcription of rpoe, codi ...200919486159
an evaluation of methods for the isolation of yersinia enterocolitica from surface waters in the grand river watershed.yersinia enterocolitica is a foodborne pathogen, but the importance of water as a route of exposure for human infection is not well known. y. enterocolitica isolation methods were developed primarily for food and clinical samples, and may not be effective for use with environmental samples. the objective of this study was to assess the recovery of y. enterocolitica from surface water used for drinking water treatment. four enrichment broths and an alkaline treatment protocol were compared for th ...200919491491
distribution of pathogenic yersinia enterocolitica in china.yersinia enterocolitica (1,295 strains) was isolated from diarrhea patients, livestock, poultry, wild animals, insect vectors, food, and the environment. they were studied for epidemiology distribution using bacterial biochemical metabolism tests, their virulence genes, and pulsed-field gel electrophoresis (pfge) sub-typing. the data showed that 416 of the 1,295 strains were pathogenic, where the pathogenic chinese isolates were of serotypes o:3 and o:9. these two serotypes were found in livesto ...200919575249
prevalence of enteropathogenic yersinia in estonian, latvian, and russian (leningrad region) pigs.tonsils of 457 fattening pigs from estonia (n = 151), latvia (n = 109), and the leningrad region of russia (n = 197) were collected between 2004 and 2007 to study the prevalence of enteropathogenic yersinia in slaughter pigs. yersinia enterocolitica and yersinia pseudotuberculosis were isolated by selective and cold enrichment. pathogenic y. enterocolitica and y. pseudotuberculosis were identified by pcr targeting the chromosomal genes ail and inv, respectively. the presence of the virulence pla ...200919580449
treatment of whole blood (wb) and red blood cells (rbc) with s-303 inactivates pathogens and retains in vitro quality of stored rbc.a pathogen inactivation (pi) process has been developed using the frangible anchor linker effector (frale) compound s-303. a series of experiments were performed in whole blood (wb) to measure the level of viral and bacterial inactivation. the results showed that 0.2mm s-303 and 2mm glutathione (gsh) inactivated >6.5 logs of hiv, >5.7 logs of bluetongue virus, >7.0 logs of yersinia enterocolitica, 4.2 logs of serratia marcescens, and 7.5 logs of staphylococcus epidermidis. recent development for ...201019995680
role of lipid a acylation in yersinia enterocolitica virulence.yersinia enterocolitica is an important human pathogen. y. enterocolitica must adapt to the host environment, and temperature is an important cue regulating the expression of most yersinia virulence factors. here, we report that y. enterocolitica 8081 serotype o:8 synthesized tetra-acylated lipid a at 37 degrees c but that hexa-acylated lipid a predominated at 21 degrees c. by mass spectrometry and genetic methods, we have shown that the y. enterocolitica msbb, htrb, and lpxp homologues encode t ...201020385763
first analysis of a bacterial collagen-binding protein with collagen toolkits: promiscuous binding of yada to collagens may explain how yada interferes with host processes.the yersinia adhesin yada mediates the adhesion of the human enteropathogen yersinia enterocolitica to collagens and other components of the extracellular matrix. though yada has been proposed to bind to a specific site in collagens, the exact binding determinants for yada in native collagen have not previously been elucidated. we investigated the binding of yada to collagen toolkits, which are libraries of triple-helical peptides spanning the sequences of type ii and iii human collagens. yada b ...201020439473
human complement factor h binds to outer membrane protein rck of salmonella.serum resistance, or resistance to complement-mediated killing, is a key virulence property of microbial pathogens. rck is a 17-kda outer membrane protein encoded on the virulence plasmid of salmonella enterica serovars typhimurium and enteritidis. when expressed in either escherichia coli or s. enterica typhimurium, rck confers serum resistance independent of lps length. recently, the rck homolog from yersinia enterocolitica, ail, has been shown to bind the complement regulatory protein factor ...201020622116
chronic yersiniosis due to defects in the tlr5 and nod2 recognition pathways.infection with yersinia enterocolitica leads to a self-limiting disease, but in a small number of cases a protracted course can develop. the host genetic factors contributing to the advancement of the disease to the chronic phase are not known. we describe a patient suffering from an abdominal inflammatory mass due to chronic yersiniosis. functional assays revealed defects in the recognition of flagellin by toll-like receptor 5 (tlr5) and of muramyl dipeptide by nod2, leading to a defective infl ...201021071776
a survey for listeria species, motile aeromonads and yersinia enterocolitica on bovine and ovine carcasses.one hundred ovine and 100 bovine carcasses in two abattoirs were sampled just after dressing for the presence of yersinia enterocolitica, listeria monocytogenes and motile aeromonads. yersinia enterocolitica was not isolated and only two samples were positive for listeria spp. in both cases, the listeria species were not normally pathogenic to man. in contrast, motile aeromonads were isolated from 81% of ovine and 35% of bovine carcasses.199416031739
non-specific seroreactions against brucella abortus in ruminants in new zealand and the presence of yersinia enterocolitica 0:9.the level of non-specific reactions found in the brucellosis serology of ruminants in new zealand was very low until july 1992. this changed when, in an export consignment of 1071 deer, 35% reacted in the brucella abortus tube agglutination test with titres varying from 50 to 200 iu. the reactors were also positive in the rose-bengal agglutination test and most of them reacted in the complement fixation test with titres varying from 10 to 80 iu. yersinia enterocolitica 0:9 was later isolated fro ...199516031844
impact of yersinia enterocolitica enteritis on disaccharidase activity and small intestinal morphology in colostrum-deprived newborn piglets.the effect of enteritis on the development of the small intestine was examined in newborn, colostrum-deprived piglets infected with a human isolate of y. enterocolitica (serotype 0:3, biotype 4) soon after birth. the piglets were killed 3 days (n = 6) or 5 days (n = 8) after infection, or antibiotic therapy was commenced on day 5 and the animals killed on day 14 (n = 5). compared with the non-infected controls, infected animals had reduced mucosal lactase and sucrase, but not maltase activity, w ...199716031944
complete genome sequence of a yersinia enterocolitica "old world" (3/o:9) strain and comparison with the "new world" (1b/o:8) strain.yersinia enterocolitica is a heterogeneous bacterial species with a wide range of animal reservoirs through which human intestinal illness can be facilitated. in contrast to the epidemiological pattern observed in the united states, infections in china present a pattern similar to those in european countries and japan, wherein "old world" strains (biotypes 2 to 5) are prevalent. to gain insights into the evolution of y. enterocolitica and pathogenic properties toward human hosts, we sequenced th ...201121325549
complete genome sequence of yersinia enterocolitica subsp. palearctica serogroup o:3.we report here the first finished and annotated genome sequence of a representative of the most epidemiologically successful yersinia group, y. enterocolitica subsp. palearctica strain y11, serotype o:3, biotype 4. this strain is a certified type strain of the german dsmz collection (dsm no. 13030; yersinia enterocolitica subsp. palearctica) that was isolated from the stool of a human patient (h. neubauer, s. aleksic, a. hensel, e. j. finke, and h. meyer. int. j. med. microbiol. 290:61-64, 2000) ...201121296963
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