production of heat-stable, methanol-soluble enterotoxin by yersinia isolates of yersinia enterocolitica serotype 0:8, recovered during an outbreak of gastrointestinal illness, were examined for enterotoxin production. all seven strains were enterotoxigenic in the suckling mouse model, and three of five isolates tested produced keratoconjunctivitis in the guinea pig eye model (sereny test). enterotoxin was detected in broth supernatant fluid after 12 h of incubation at 25 degrees c. the toxin was not inactivated by exposure to 121 degrees c for 30 min or by ...197939891
antigenicity of protein and lipopolysaccharide from yersinia enterocolitica.the rabbits infected intravenously with viable yersinia enterocolitica (y.e.) produced the precipitating antibodies against a protein component as well as lipopolysaccharide of the organism. the production of the precipitins against protein antigen persisted for a long period of time even after the o-agglutinins had disappeared. the rabbits infected orally with the organism produced only the precipitins against protein antigen, and the amount of the antibody was equal to or greater than that of ...1979119613
assessment of typhoid vaccines by using the intraperitoneal route of challenge.present laboratory tests for human typhoid vaccines use an intraperitoneal route of challenge given 7 days after injection of increasing doses of standard and test vaccines by the same route. in studies reported here, groups of b6d2 mice were vaccinated intraperitoneally with 2 x 10(8) acetone-killed salmonella typhi ty2, with the vi antigen-free variant o-901, or with yersinia enterocolitica and serratia marcescens suspensions. other groups of mice received 200 mug of purified s. typhi or s. ma ...1977332627
efficacy of cold enrichment techniques for recovery of yersinia enterocolitica from human stools.stool specimens from children with gastroenteritis and their household contacts were cultured for yersinia enterocolitica by direct plating onto routine laboratory media. these stools were also inoculated into phosphate-buffered saline and subcultured to the same media after 1 day or 3 weeks of incubation at 4 degrees c. y. enterocolitica was isolated from 174 index cases and 34 household contacts. one hundred eighty-one isolates were of serotype o:3, and the remaining 21 belonged to other serot ...1979387818
human gastrointestinal infections by yersinia an average-sized peripheral hospital in belgium, y. enterocolitica has been isolated from 1.1% of stool cultures, using an unmodified routine technique. in a group of unselected patients undergoing appendicectomy, 1.3% of patients had a positive culture, either from the mesenteric lymph nodes or the appendix, or from both sources. in the same group of patients, 1.7% of cultures grew a salmonella. in more than half of the patients, the diagnosis of mesenteric adenitis was made during the surgi ...1979394918
comments on the ecology and epidemiology of yersinia enterocolitica in czechoslovakia.on the basis of the information disseminated by the reference laboratory in 1968, the microbiological diagnosis of yersinia enterocolitica (ye) was gradually mastered by all czechoslovak microbiological laboratories. the most frequent serotype isolated in man was ye 03. isolation predominated from cases of diarrhoea, less often from appendectomies, and only one case of septicaemia was described. usually only sporadic cases occur and outbreaks are rare. a few strains of ye 05 were found in diarrh ...1979535370
epidemiological and clinical aspects of human yersinia enterocolitica infections in the period from 1963 to 1975 data were collected on 1,781 isolates of y. enterocolitica from human infections in belgium. there was an uneven spread of the cases over the country, but this could simply reflect the distribution and activity of medical laboratories. serotype 3 dominated (89.2%) followed by serotype 9 (8.6%). less than 1% of all isolates were from nonenteral sources. gastroenteritis was the diagnosis in 86.8% of patients, while 10.6% were suffering from an appendicitis-like synd ...1979535373
prevalence of unique yersinia enterocolitica in the area of the mount sinai hospital, new york, n.y.the incidence of infection due to yersinia enterocolitica is unknown in the united states as well as in a given geographic locale such as new york city. comparison of 10 y. enterocolitica strains recovered at nine hospitals in the new york city area to 22 isolated at the mount sinai hospital revealed striking differences in clinical presentation of disease, biochemical behavior of the isolates and their serological profiles. the 10 strains were from patients with 'classical syndromes', were bioc ...1979535396
atypical yersinia enterocolitica: clinical and epidemiological parameters.infections due to biochemically typical yersinia enterocolitica usually present as gastroenteritis, mesenteric lymphadenitis, terminal ileitis, and septicemia often with visceral abscesses. in these instances, the isolates have been biochemically typical and of well-established serotypes, namely 0:3 or 0:9 and, in the united states, 0:5 or 0:8. the recovery, recognition, and significance of biochemically and serologically atypical y. enterocolitica in human infections has proceeded more slowly. ...1978670380
yersinia enterocolitica: serotypes and biotypes isolated from humans and the environment in quebec, canada.thirty-one strains of yersinia enterocolitica were isolated from food and surface water. during the period of january 1975 to june 1977, 157 strains from 143 human cases were also isolated. among the different serotypes from nonhuman sources, serotypes 6,30 and 4,32 were the most common. serotype 4,32 was present only in food and not in water. serotype 3 was isolated only from humans. among the different serotypes from human cases, 73.9% belonged to serotype 3. only serotype 3 was isolated from ...1978670388
isolation of yersinia enterocolitica from surface and well waters in ontario.yersinia enterocolitica was isolated from forty-three surface- and well-water samples and one municipal supply. in many cases these samples were negative for coliform bacteria. after incubation of membrane filters on m-endo agar les at 35 degrees c for total coliform analysis, filters were held an additional 24 h at room temperature. this method was more productive for isolation of waterborne types of y. enterocolitica than was cold enrichment in buffer alone or in combination with modified rapp ...1978709433
enzyme-linked immunosorbent assay (elisa) for titration of antibodies against brucella abortus and yersinia enzyme linked immunosorbent assay (elisa) using phenolwater extracted lipopolysaccarides as antigen was used for detection and quantitation of antibodies against brucella and yersinia bacteria in rabbit antisera. elisa was found to be from ten to hundred-fold more sensitive than the commonly used tube agglutination assay (widal). in addition, both direct and inhibition assays using elisa revealed antigenic differences between brucella abortus and yersinia enterocolitica o-group v, previously ...1976826112
yersinia enteritis and enterocolitis: gastroenterological aspects.the clinical, radiological, and endoscopic aspects of yersinia enterocolitica infections in man were studied in a group of 37 adult patients observed in a 4-year period in a single gastrointestinal unit. the diagnosis was based on isolation of the bacterium in all but 1 patient. abdominal pain and diarrhea were the most prominent symptoms, occurring in 80% of the patients. a syndrome simulating appendicitis was observed in 40%. the duration of symptoms before diagnosis varied from 1 or 2 weeks i ...1977830572
isolation of yersinia enterocolitica from danish swine and dogs.seventeen strains of yersinia enterocolitica were isolated on examination of the caecal contents from 100 bacon pigs at slaughter. in another material consisting of 222 pigs with various diseases, the bacterium was found in 12 cases. three out of 40 dogs were positive for the bacterium. seven of the porcine and one of the canine strains belonged to serotype o 3, which is a human pathogen.1976970137
human serum antibodies against heat-stable antigens from yersinia enterocolitica.human sera, 200 from blood donors and five from patients with infection caused by yersinia enterocolitica serotype 3 (y.e. 3), were tested by indirect haemagglutination for antibodies against antigens from y.e. serotypes 3 and 9. erythrocytes were sensitized using extracts prepared by heating of the bacteria at 100 degrees c for 60 min. all sera tested showed antibody activity against antigen from both y.e. serotypes. in blood donors, the titres ranged from 4 to 512, and in patients from 512 to ...19751180060
animal model of human disease. yersinia enteritis. animal model: oral yersinia enterocolitica infection of mice. 19751211427
[experimental pathogenicity of "yersinia enterocolitica" for athymic nude mice (author's transl)].amongst the 4,500 strains of our collection of yersinia enterocolitica usually non-pathogenic for laboratory animals, 5 or 6 strains appeared to be naturally pathogenic for mice. using these strains and non-pathogenic strains representing more than 90 per cent of human isolates in the world (biotype 4, serotype 0:3, phagocyte viii; biotype 2, serotype 0:9, phagotype x3), the pathogenicity for cyclophosphamid treated mice and athymic nude mice has been tested. highly pathogenic strains killed con ...19751217786
biotypes and sensitivity screening yersinia enterocolitica as an infective agent in man and swine in nigeria.diarrhoeic faecal samples from 210 humans and 192 swine were screened for yersinia enterocolitica in 1990. ten and 8 y. enterocolitica strains were isolated from pig and man, respectively. the isolates were found to belong to wauter's biotypes 1, 2, 3 and 4. biotype 2 was isolated mainly from human stool samples. biotype 3 was found only in swine while biotypes 1 and 4 were isolated from both man and swine. all the 18 strains showed varying degrees of sensitivity to antibiotics used in this inve ...19921301624
stimulation of synovial fluid mononuclear cells with the human 65-kd heat shock protein or with live enterobacteria leads to preferential expansion of tcr-gamma delta+ lymphocytes.t lymphocyte responses to heterologous or self 65-kd heat shock protein (hsp) have been implicated in the pathogenesis of various forms of arthritis. to delineate the relationship of 65-kd hsp to different synovial fluid (sf) t cell subsets, we stimulated synovial fluid (sfmc) and peripheral blood mononuclear cells (pbmc) from patients with different inflammatory rheumatic diseases and from healthy controls with human or mycobacterial 65-kd hsp, tetanus toxoid (tt), heat-killed or live yersinia ...19921387595
detection of pathogenic yersinia enterocolitica by polymerase chain reaction and digoxigenin-labeled polynucleotide probes.yersinia enterocolitica is widespread in nature, but only a few bioserotypes are involved in human infections. pigs are considered to be the major reservoirs of pathogenic strains. it is essential to have an accurate and rapid method for the detection of pathogenic yersiniae. to achieve this objective, 19-base synthetic oligonucleotide primers were used in a polymerase chain reaction (pcr) to detect the ail gene (which is conserved only in pathogenic strains) in strains of y. enterocolitica and ...19921400967
laboratory investigation of virulence among strains of yersinia enterocolitica and related species isolated from pigs and pork products.eighty strains of yersinia enterocolitica and related species isolated from slaughtered pigs and pork products were tested for possession of virulence-associated phenotypes by employing 12 in vivo and in vitro assays. the isolates could be broadly divided into two groups: (i) strains belonging to pathogenic bioserotypes of y. enterocolitica that displayed virulence-associated characteristics in most or all assays and (ii) strains belonging to y. enterocolitica biotype 1a and to related species t ...19921521193
lcrf is the temperature-regulated activator of the yada gene of yersinia enterocolitica and yersinia pseudotuberculosis.the virulence plasmid of human pathogenic yersinia species, pyv, encodes secreted proteins, yop proteins, and an outer membrane protein, yada. yada has been associated with binding to a variety of substrates and with interference with host defense. yada is regulated by temperature and is expressed only at 37 degrees c. unlike the yop regulon, the yada gene is not under ca2+ regulation. here, we show that lcrf (virf), the temperature-regulated activator of the yop regulon, also acts as an activat ...19921548243
[comparison of yersinia enterocolitica strains isolated from swine and humans in guipuzcoa].yersinia enterocolitica biotype 4, serotype 3 is a pathogenic bacteria for men. its most likely reservoir are pigs. we have compared 48 y. enterocolitica serotype 0:3 strains isolated from pig's tonsils samples as well as porcine-derived food with 301 y. enterocolitica serotype 0:3 isolate from human stool's samples. all strains are positive for virulence tests. yersinia enterocolitica serotype 0:3, biotype 4, phage-type viii was nearly the only single pathogenic strain isolated from both origin ...19921606219
interactions between yersinia enterocolitica and the host with special reference to virulence plasmid encoded adhesion and humoral immunity.this thesis is based on 8 publications and a review of the literature. the aim was to summarize present knowledge on molecular components of yersinia enterocolitica involved in (i) adhesion, with special reference to plasmid encoded factors and (ii) induction of antibodies in the host, and the quantitation of these two phenomena. adhesion of y. enterocolitica was quantitatively studied by methods that measured binding to cultured epithelial cells, mucosal constituents immobilized on polystyrene, ...19921611921
immunoreactivity to yersinia enterocolitica antigens in patients with autoimmune thyroid disease.recent reports have suggested that yersinia enterocolitica proteins encoded by a 72-kilobase virulence plasmid (known as release proteins and now identified as yop2-5) are antigens recognized specifically by patients with graves' disease and of potential etiological importance in this disorder. to examine this hypothesis, we evaluated immune responses to yop in patients with autoimmune thyroid disease and in normal controls. humoral responses to yersinia were assessed using western blots of crud ...19921619022
[studies on the serogroup of yersinia enterocolitica. ii. selection and establishment on serogroup reference strains of yersinia enterocolitica].since 1980, we have collected 1120 strains of yersinia enterocolitica, from the different parts of china. these strains have been obtained from various sources in man, animals and natural environment accompanied by their clinical or ecological information of yersinia enterocolitica. the results of our tests have shown that the 747 strains have exhibited the clinical morphological and biochemical characteristics of yersinia enterocolitica. through comparing under the same conditions, out of the 7 ...19911709767
serum bactericidal activity for yersinia enterocolitica in hemodialysis patients: effects of iron overload and deferoxamine.human serum has been shown to be bactericidal for most strains of yersinia enterocolitica. systemic y enterocolitica infections have been reported in iron-overloaded hemodialysis patients treated with deferoxamine. both iron and deferoxamine are known to enhance the growth of y enterocolitica. we inoculated sera from 12 hemodialysis patients whose serum ferritin levels ranged from 26 to 6,855 micrograms/ml (ng/ml), as well as three controls, with yersinia organisms. after latencies of 0 to 24 ho ...19921739096
virulence of yersinia spp. strains isolated from non-human sources.1. strains of yersinia enterocolitica, y. intermedia, y. frederiksenii and y. kristensenii from non-human sources were examined for virulence factors. four of these strains were positive for autoagglutination, three were calcium dependent at 37 degrees c, three produced lipase, five had the ability to bind congo red, six had pyrazinamidase activity, and two had a 42 mda plasmid. 2. one strain (y. enterocolitica 0:5) was lethal for mice and had the ability to invade guinea pig eyes and hela cells ...19911797265
[functional state of thrombocytes in yersinia infection].as many as 94 patients with yersiniosis were examined for the morphofunctional status of platelets (aggregation, disaggregation, endo-exocytosis and platelet forms). the data indicate that platelet from patients with yersiniosis possess a high ability for aggregation and a low ability for disaggregation. according to the experimental and clinical data, emoxipine can restore disturbed function of human platelets.19911801465
[an immunoenzyme test system for the detection of antibodies to campylobacter].an enzyme immunoassay system for the detection of antibodies to bacteria of the genus campylobacter in human blood serum has been developed. the system is based on the use of ethanol-treated c. jejuni and c. coli whole cells as antigen. the study of sera obtained from healthy donors in this assay has made it possible to establish the value of the tentative diagnostic titer: 320.19911801490
first isolation of yersinia enterocolitica serotype o:8 in japan.a 4-year-old japanese boy was infected with yersinia enterocolitica serotype o:8, h:befiv, biotype 1b, phage type xz, restriction endonuclease analysis of plasmid dna type b, restriction endonuclease analysis of chromosomal dna type 8. he presented with acute gastroenteritis with elevated body temperature (40 degrees c), rigor, and shivers. the diagnosis was septicemia. this is apparently the first report of this serotype from a human infection outside north america.19911812854
yersinia enterocolitica biotype 2 serotype o9 septicaemia in a previously fit man, raw goats' milk having been the apparent vehicle of infection: a cautionary tale.a previously fit 66-year-old man presented with a 2 weeks' history of malaise, fever and vomiting which led to a septicaemic illness. yersinia enterocolitica biotype 2 serotype o9 was isolated from the patient's blood and from raw goats' milk remaining in a bottle after the patient had consumed some of the contents. he also produced antibodies to this serotype. careful history taking, however, revealed that the bottle of milk had been purchased after the patient became ill. milk from the same bo ...19911885916
aspects of the epidemiology of yersinia enterocolitica: a review.a review of works concerning different aspects of the epidemiology of human pathogenic yersinia enterocolitica biogroup iv/serogroup o:3 (y. enterocolitica o:3) is given. to investigate the epidemiology of y. enterocolitica o:3 in danish herds of pigs, tonsil swabs from 2218 freshly slaughtered pigs originating from 99 herds were examined. the organism was isolated from 25% of the pigs and from 82% of the herds. no herd management factor could be associated with the presence of the organism. the ...19911892741
urease-negative strains of yersinia enterocolitica and related species isolated from human and nonhuman sources. 19911935112
[viral infections associated with intestinal invagination].intestinal invagination is rather frequently associated with local tumors, adenovirus infections, and occasionally with yersinia enterocolitica. however, in many cases the etiology is not known. we have studied 88 children with intestinal invagination in whom cellular cultures of hep 2 and pulmonary fibroblasts of human embryo have been inoculated with a fecal sample. the clinical reports have been reviewed retrospectively. in 19 patients (21.59%) we obtained an adenovirus and in 12 patients (13 ...19901967100
[liver abscesses due to yersinia enterocolitica].a 41-year-old man with type ii diabetes for the past five years had for three weeks been suffering from high fever (up to 40 degrees), feeling of pressure in the upper abdomen, loss of weight, lack of appetite and increasing weakness. ultrasound examination as an out-patient was suspicious of diffuse liver metastases from an unknown primary tumour. ultrasonography and computed tomography after hospitalization suggested multiple liver abscesses. fine-needle biopsy grew yersinia enterocolitica. in ...19911997307
inhibition of plasmid-encoded adhesion of yersinia enterocolitica by non-immunoglobulin fraction of human milk.the inhibitory effect of human milk on plasmid-encoded adhesion of yersinia enterocolitica to rabbit ileal brush border membrane vesicles immobilized on polystyrene was evaluated. adhesion was reduced to about 50% after incubation of equal volumes of bacteria and undiluted human milk, but a significant reduction was also detectable after incubation with milk samples at a final 1:10 dilution. removal of immunoglobulins from human milk by means of immunoadsorption did not change the inhibitory eff ...19912069808
[enteropathogenic yersinias: pathogenicity factors and new diagnostic methods].a limited number of serotypes of yersinia enterocolitica and y. pseudotuberculosis is enteropathogenic for humans. during the last decade molecular biology contributed significantly to the understanding of the pathogenicity of these enteric agents. all human pathogenic yersiniae harbour a 70-kilobase plasmid which is essential for virulence expression. presently, thirteen plasmid-encoded polypeptides have been identified. two of them have been identified as true outer membrane proteins and at le ...19902076901
a permanent endoparasite of man. 1. the silent zoogleal/symplasm/l-form phase. 19902084495
recombinant erythropoietin reverses polymorphonuclear granulocyte dysfunction in iron-overloaded dialysis patients.iron overload increases the risk of bacterial infection in dialysis patients, partly by impairing functions of the polymorphonuclear granulocytes (pmns). pmn defence was studied sequentially in haemodialysis patients with transfusional haemosiderosis, treated for 6 +/- 1.5 months (n = 8) to 13 +/- 1.7 months (n = 4) with recombinant human erythropoietin (rhuepo). over this period, signs of iron overload (increased serum ferritin and serum iron) improved, and stainable iron disappeared in pmns. s ...19902130296
effect of acute yersinia enterocolitica infection on small intestinal ultrastructure.the purpose of this study was to assess the jejunal and ileal brush border injury caused by yersinia enterocolitica and to correlate these alterations with functional abnormalities. weanling rabbits infected with 10(10) organisms of a human pathogenic y. enterocolitica strain were compared with control and pair-fed, sham-treated animals. on day 6, infection resulted in a diffuse decrease in brush border enzyme activities in the small intestine and villus atrophy and crypt hyperplasia in the ileu ...19902186949
precipitation of struvite in urine medium by urease-positive and urease-negative yersinia strains.yersinia strains either urease-positive or urease-negative were examined for precipitation of struvite in human urine at 25 and 37 degrees c. all urease-positive strains and 8 out of 10 urease-negative strains showed the ability to produce these crystals. incubation time required for precipitation was longer for urease-negative strains and quantity of struvite formed was higher in urease-positive ones. regarding incubation temperature, no significant influence has been observed.19902219568
the influence of properties encoded by the yersinia virulence plasmid on adhesion of yersinia enterocolitica to ileal brush border membrane vesicles.the influence of plasmid-associated cell surface structures on the ability of yersinia enterocolitica to bind to ileal brush border membrane vesicles (bbvs) was investigated. rabbit or human bbvs were immobilized on polystyrene microtiter plates and adhesion of radiolabeled cells of y. enterocolitica was determined. strains of pathogenic serotypes carrying the yersinia virulence plasmid (pyv+), as well as their isogenic plasmid cured derivatives (pyv-), adhered to immobilized bbvs, but adhesion ...19902245012
monoclonal antibodies against escherichia coli heat-stable toxin (sta) and their use in a diagnostic st ganglioside gm1-enzyme-linked immunosorbent monoclonal antibodies (mabs) against heat-stable enterotoxin (st) from a human escherichia coli isolate were prepared and evaluated for their usefulness in an st immunodetection assay, the st ganglioside gm1-enzyme-linked immunosorbent assay (elisa). this assay is based on the ability of sta, as present in, for example, culture filtrates from st-producing e. coli, to inhibit specific anti-st antibody from binding to solid-phase-bound st ganglioside (gm1-bound st-cholera b subunit). four of ...19862429984
immunoblot analysis of human igm, igg and iga responses to plasmid-encoded antigens of yersinia enterocolitica serovar o3.human igm, igg and iga responses after infection with yersinia enterocolitica serovar o3 were studied by immunoblotting sera against whole-cell homogenates of a plasmid-containing strain of y. enterocolitica o3 and a plasmid-free strain derived from it; each strain was grown in conditions expressive for the plasmid. the antibodies observed were directed against several plasmid-encoded polypeptides. the response against different bacterial components decreased uniformly with time and the persisti ...19872443704
[current problems in the microbiological control of food].the paper deals with a number of aspects of current interest and significance on the subject of the microbiological testing of food with particular reference to the role of research institutes. the main objectives of microbiological testing of food are essentially two: (i) to establish the absence of a human health hazard due to microbial contamination of food (food safety); and (ii) to define the quality standard of food (food quality). as far as the first of these objectives is concerned, the ...19892483651
thyrotrophin (tsh)-binding proteins in bacteria and their cross-reaction with autoantibodies against the human tsh receptor.a screen of a range of bacteria normally found in gut flora identified eight with the ability to bind tsh specifically. these included the previously reported yersinia enterocolitica, gram-positive, gram-negative, pathogenic and commensal organisms. eleven preparations of tsh-receptor autoantibodies strongly able to displace 125i-labelled tsh from the mammalian tsh receptor differed in their ability to displace the tracer from binding to bacterial extracts. none could displace the tracer from e. ...19892754380
serotype-related hep-2 cell interaction of yersinia enterocolitica.the ability of human clinical isolates of yersinia enterocolitica to interact with hep-2 cells was found to vary considerably between the serotypes o:3, o:8, and o:9. although all three serotypes adhered initially to the cell surface, regardless of incubation temperature of the bacterial inoculum or presence of the 40- to 48-megadalton virulence plasmid, the ability to localize intracellularly was not uniformly expressed. by using a combined light optical method based on differential interferenc ...19862870030
potent antagonism of escherichia coli, bacteroides ovatus, fusobacterium varium, and enterococcus faecalis, alone or in combination, for enteropathogens in anaerobic continuous flow cultures.interactions between representative strains of four predominant resident bacteria of the human colon, escherichia coli, enterococcus faecalis, bacteroides ovatus, and fusobacterium varium, and strains of seven enteropathogens, yersinia enterocolitica, shigella flexneri, salmonella typhimurium, vibrio parahaemolyticus, v. cholerae serogroup non o1, staphylococcus aureus, and clostridium perfringens, were examined in studies with an anaerobic continuous flow culture system and medium resembling th ...19862875188
[occurrence of human pathogenic yersinia enterocolitica strains in drinking water]. 19862940493
[in vitro activity of an extract of yersinia enterocolitica 0:3 biotype 4 on lymphoblastic transformation].effects of an extract from y. enterocolitica 0:3 biotype 4 on mouse splenocytes and human lymphocytes cultures were examined. low doses had a mitogenic action on splenocytes, while high doses inhibited tritiated thymidine incorporation into the cells. with human lymphocytes only inhibitory effect was observed.19853160439
lack of hybridization between yersinia enterocolitica and hla-b27 homology was observed between yersinia enterocolitica o:3 and hla-b27 at dna level when yersinia enterocolitica chromosomal probes were hybridized with human hla-b27 positive leukocyte dna or in the hybridization of yersinia dna with hla-b27 specific probe. our results do not exclude the existence of molecular mimicry between yersinia proteins and hla-b27 antigen, since the crossreactive epitope might be a conformational determinant not detected with hybridization.19883262758
isolation of campylobacter spp. and yersinia enterocolitica from domestic animals and human patients in kenya.rectal swabs or faecal samples from 992 domestic animals and 97 human patients in the nairobi region were cultured for thermophilic campylobacter species and yersinia enterocolitica. the highest isolation rate of campylobacters was obtained from diarrhoeic pigs (55.1%), followed by healthy chicken (51.5%), diarrhoeic dogs (47.2%), healthy pigs (44.0%), healthy ducks (29.4%), healthy goats (6.3%), healthy cattle (5.8%), diarrhoeic humans (3.1%), and healthy sheep (2.0%). only one strain of y. ent ...19883345259
[initial isolation and characterization of yersinia enterocolitica in human feces in argentina].the isolation of yersinia enterocolitica from a child's feces with an acute diarrhea is reported for the first time in argentina. the strain was classified as belonging to the biotype 4 of wauters, serotype o:3, phage type viii. the virulence-associated test of autoagglutination, calcium dependency and production of heat-stable enterotoxin were positive, whereas neither lethality for the adult mouse by oral route, nor invasiveness by using the serény test in the mouse, were demonstrated. a plasm ...19873503312
incidence of human infection with yersinia enterocolitica serotypes o3, o8, and o9 and the use of indirect immunofluorescence in diagnosis. 19863510258
detection of circulating yersinia-immunoglobulin complexes by enzyme immunoassay (eia).an enzyme immunoassay (eia) was developed for the detection of yersinia-immunoglobulin complexes of known ig class. immune complexes (ics) were attached to polystyrene microtiter plates by rabbit anti-human immunoglobulins, and the existence of yersinia enterocolitica o:3 antigens was demonstrated using fab fragments of alkaline phosphatase (ap)-conjugated antibody against the same serotype. simultaneous binding of yersinia antigens and immunoglobulins was a prerequisite for the detection of ics ...19863517176
[antibodies to yersinia enterocolitica in immunogenic thyroid diseases].in 1976 shenkman et al. revealed that in patients with thyroid disorders antibodies against yersinia enterocolitica could be demonstrated in increased frequency. in 1983 ingbar et al. first established that the gram-negative bacterium yersinia enterocolitica shows on its surface saturable binding sites for thyrotropin (tsh). if such binding sites resemble immunologically human tsh receptors this would indicate that tsh receptor antibodies could be produced in selected individuals having been inf ...19873618088
prevalence of antibodies to human serogroups of yersinia enterocolitica in blood donors in nigeria. 19863652191
virulence-plasmid is associated with the inhibition of opsonization in yersinia enterocolitica and yersinia pseudotuberculosis.plasmid-cured variants of virulent strains of yersinia enterocolitica and y. pseudotuberculosis were obtained by selection after growth in calcium-deficient medium. to obtain antigen preparations consisting of whole bacteria the original plasmid-containing strains and the plasmid-cured variants were grown in conditions favouring expression of the temperature-inducible outer membrane proteins of yersinia (yop) (37 degrees c, calcium-deficient culture medium). the presence or absence of the yop on ...19873652518
human antibody response to individual chromosome- and plasmid-encoded antigens of yersinia enterocolitica 0:3. 19873665508
attempt of yersinia enterocolitica isolation from human feces in stool samples, collected in a fishing village of senegal from 72 apparently healthy subjects and from 36 patients with gastrointestinal disorders, were examined for the presence of y. enterocolitica. after 1, 2, 3 weeks of cold enrichment with pbs 1/15m, ph 7.6, plating was performed on macconkey agar after use of the alkali method. no yersinia strains were isolated.19873691753
inhibition of yersinia enterocolitica serotype o3 by natural microflora of pork.yersinia enterocolitica serotype o3 did not grow but did survive in inoculated raw ground pork kept at 6 and 25 degrees c. the antagonistic effect of microbial flora, especially hafnia alvei and environmental yersinia organisms, on the growth of y. enterocolitica serotype o3 in raw ground pork was evident. these results were supported by evidence of the inhibition of growth of y. enterocolitica serotype o3 by enterobacteriaceae, especially h. alvei and environmental yersinia organisms, in mixed ...19863729395
temperature-inducible surface fibrillae associated with the virulence plasmid of yersinia enterocolitica and yersinia pseudotuberculosis.when cultivated at 37 degrees c in static broth, human clinical isolates of yersinia enterocolitica (serogroups o:3, o:8, and o:9) and yersinia pseudotuberculosis (serogroup o:iii) produced numerous nonflagellar surface appendages, which appeared as a lawn of fine fibrillae, each having a diameter of 1.5 to 2.0 nm and a length of 50 to 70 nm. cultivation at 22 degrees c resulted in complete disappearance of the fibrillae. the phenotypic expression of these appendages was correlated with the pres ...19853881355
pyrazinamidase activity in yersinia enterocolitica and related organisms.pyrazinamidase activity was tested in 381 yersinia strains from various ecological and geographical origins and belonging to the following species: y. enterocolitica (five biogroups), y. intermedia, y. frederiksenii, y. kristensenii, y. aldovae, y. pseudotuberculosis, and y. pestis. the pyrazinamidase test was negative (pyz-) in all bioserogroups of y. enterocolitica, in which is usually harbored the virulence plasmid, and was involved in human or animal diseases. y. pseudotuberculosis and y. pe ...19854008626
assessment of plasmid profile, exoenzyme activity, and virulence in recent human isolates of yersinia enterocolitica.we examined a group of 23 recent clinical isolates of yersinia enterocolitica recovered from symptomatic patients residing in the new york, n.y. area. these isolates were tested for the presence of plasmids, exoenzyme activity, mouse lethality, and phenotypic properties postulated to correlate with virulence. of the 23 isolates, 17 harbored a 60- to 65-kilobase (kb) plasmid. six isolates were lethal for white mice, showed the phenotypic markers of autoagglutination and calcium dependence for gro ...19854044802
a patient with hemosiderosis and multiple liver abscesses due to yersinia enterocolitica.a 72 yr-old man presented with clinical features suggestive of disseminated malignancy, and at autopsy was found to have hemosiderosis and multiple liver abscesses due to yersinia enterocolitica. the literature is reviewed, with emphasis on the role of abnormal iron metabolism in disseminated infection with this organism.19854069774
isolation of yersinia enterocolitica and related species from foods in france.the occurrence of yersinia enterocolitica and related species (y. intermedia, y. frederiksenii, y. kristensenii) in foods from france was investigated by using different enrichment procedures. initially, seven procedures were evaluated with pork products. these methods included a cold preenrichment in yeast extract-rose bengal broth or in phosphate-sorbitol-bile medium, followed by selective enrichment either in pastone-sucrose-tris-azide broth, in modified rappaport broth, or in bile-oxalate-so ...19854083878
studies on yersinia enterocolitica with special reference to bacterial diagnosis and occurrence in human acute enteric disease. 19694190521
vibriocidal antibodies induced by yersinia enterocolitica serotype ix.yersinia enterocolitica serotype ix has been found to stimulate the production of vibriocidal antibody against vibrio cholerae, particularly in inaba serotype, in experimental rabbits and infected man to a significant degree. this activity could be absorbed by both inaba and yersinia antigens from anti-yersinia sera, but yersinia antigen could not absorb vibriocidal activity from the anti-cholera sera, indicating a unilateral relation.vibrio agglutinating antibody, particularly against ogawa, in ...19724501837
[yersinia enterocolitica, isolated in human stool specimen]. 19734786018
[studies of human infection with yersinia enterocolitica]. 19704923764
[human infection with "yersinia enterocolitica"]. 19665339619
yersinia enterocolitica in human feces. 19695371646
[human infection caused by yersinia pseudotuberculosis (malassez and vignal bacillus) and yersinia enterocolitica]. 19695380346
yersinia enterocolitica (pasteurella x) in human enteric infections. 19665957419
spontaneous yersinia enterocolitica peritonitis in idiopathic hemochromatosis.we report a case of spontaneous yersinia enterocolitica peritonitis revealing idiopathic hemochromatosis in a 62-yr-old man with fever and diarrhea. blood and stool cultures were repeatedly negative, but yersinia enterocolitica serotype 9 was isolated from ascitic fluid and serum agglutinins against this organism were positive at a dilution of 1:2560. moreover, a serum brucella agglutination test was positive at a dilution of 1:320. antibiotic therapy with doxycycline led to a rapid clinical imp ...19846489703
o:13a,13b, a new pathogenic serotype of yersinia enterocolitica.forty strains of a new yersinia enterocolitica serotype isolated in the united states from nonhuman primates and humans were characterized as serotype o:13a,13b. most of the human strains were isolated from a large multistate outbreak of milkborne enteritis. a common antigenic factor between this new serotype and four previously described y. enterocolitica (o:7,13; o:18; o:44; and o:44,45) led to recharacterization of the latter as serotypes o:7,13a,13b; o:18,13b; o:44,13a; and o:44,13a,45.19846511868
[isolation of yersinia enterocolitica from drinking water in south bavaria].2429 samples of drinking water supplies in south bavaria were examined for yersinia between october 1982 and march 1983. yersinia enterocolitica was isolated from 82 of 1650 samples of central water supplies and from 42 of 779 samples of decentralized water supplies. only three of these strains were serotype o3 but indole-positive and different by that from the indole-negative human pathogenic serotype o3 which is most frequent in western europe. no strain was serotype o9. y.e. was more often is ...19846524147
bacterial shock due to transfusion with yersinia enterocolitica infected blood.a fatal case (a 55-yr-old man) of bacterial shock and sepsis following a transfusion with erythrocytes infected with yersinia enterocolitica serotype 03, is reported. the blood donor had slight diarrhea 6 days before the blood donation. a serum sample from the donor showed high titre of both igg and igm antibodies against y. enterocolitica 03, indicating a recent infection. y. enterocolitica 03 was isolated from blood cultures from the patient. the remaining portion of the transfused erythrocyte ...19846528227
bacteria associated with crabs from cold waters with emphasis on the occurrence of potential human pathogens.a diverse array of bacterial species, including several potential human pathogens, was isolated from edible crabs collected in cold waters. crabs collected near kodiak island, alaska, contained higher levels of bacteria than crabs collected away from regions of human habitation. the bacteria associated with the crabs collected near kodiak included yersinia enterocolitica, klebsiella pneumoniae, and coagulase-negative staphylococcus species; the pathogenicity of these isolates was demonstrated in ...19846742824
[first isolation of yersinia enterocolitica in several horticultural products intended usually to be consumed fresh].a survey has been carried out for the presence of yersinia enterocolitica in horticultural products. 60 samples of these vegetables have been examined among the families of compositae (lettuce, prickly lettuce, cabbage lettuce, common chicory), ombrelliferae (curly parsley, carrot, celery, fennel) and crociferae (garden cabbage). this is the first isolation of y.e. from horticultural products, being recorded 7 strains from carrots (serotype 0:6,30), 1 from curly parsley (serotype 0:4,32) and 1 f ...19826765383
[brucella titers in swine caused by yersinia enterocolitica, serotype 0:9].antibody to brucella abortus developed in two thirds of all gilts kept on a pig breeding station. systematic tests taken for the purpose of detecting clinical symptoms and of isolating brucella were negative. however, yersinia (y.) enterocolitica, serotype 0:9, was cultured from rectal swab samples which had been obtained from 31 to 78 gilts. the clinical, bacteriological, and serological tests gave rise to the assumption that the brucella titres have been caused by yersinia enterocolitica infec ...19806779771
[yersinia enterocolitica infections in southwest germany. a report on 580 bacteriologically confirmed cases (author's transl)].the bacteriological, epidemiological and clinical aspects of human yersinia enterocolitica infections are discussed with reference to 580 first isolations of yersinia enterocolitica made from 1972 to 1980 with routine procedures for salmonella/shigella diagnosis, predominantly from stool samples of patients suffering from gastrointestinal diseases.19816795458
[human infection by yersinia enterocolitica and pseudotuberculosis in spain. analysis of 80 recent cases]. 19817025130
[seasonal isolation of yersinia enterocolitica and yersinia pseudotuberculosis from tonsils of healthy slaughter pigs (author's transl)].45 strains of yersinia (y.) enterocolitica from which 36 respectively 9 belonged to o-group i or v and 28 strains of y. pseudotuberculosis from which 15 respectively 13 isolates belonged to serotype i or ii were isolated from 70 tonsils of 480 examined, clinically healthy slaughter pigs. in the tonsils of three animals were found both species together. y. enterocolitica was isolated during the whole year, but especially in the months of november (17.5%) and december (32.5%). y. pseudotuberculosi ...19817198363
characteristics of virulence in human isolates of yersinia enterocolitica.yersinia enterocolitica serotypes o:3, o:8, o:9, o:5,27, o:4,32, and o:21 were virulent as determined by autoagglutination and calcium dependency at 35 degrees c and ability to produce guinea pig conjunctivitis and mouse diarrhea.19817216491
prevalence of raised yersinia enterocolitica antibody titre in unselected, adult populations in denmark during 12 years.yersinia enterocolitica biotype 4, serotype 0:3 is by far the most common human pathogenic yersinia enterocolitica subtype in scandinavia. it is extraordinarily immunologically specific, and an elevated antibody titre greater than or equal 80-160 is known to indicate acute infection. this titre was measured in five population surveys conducted in 1967-78, including 3278 examined adult men and women. the prevalence of titre elevation greater than or equal to was 1.0% in 1967. in 1978 it was signi ...19817257868
[yersinia enterocolitica iha-test (author's transl)].612 serum specimens were examined for antibodies against yersinia enterocolitica (y.e.) with the widal technique, and an indirect (passive) hemagglutination test. the widal test was carried out with antigens of y.e. serovar 0:3 and 0:9. human erythrocytes coated with antigens of both serovarieties were used for the y.e.-iha-test. this test system showed higher agglutinin titres than the widal reaction. both methods were equivalent concerning their specificity. the y.e. iha-test is suitable for s ...19817275163
yersinia enterocolitica lung infection.a 66-year-old man had pneumonia, lung abscesses, and mediastinal adenopathy develop due to yersinia enterocolitica. there was no evidence of septicemia or acute gastrointestinal disease. diagnosis was confirmed by cultures of a transtracheal aspirate and sputum. treatment with cefamandole nafate, which had not been used previously in y enterocolitica lung disease, resulted in cure.19817283567
plasmids in yersinia enterocolitica serotypes o:3 and o:9: correlation with epithelial cell adherence in vitro.human isolates of yersinia enterocolitica serotypes o:3 (biotype 4) and o:9 (biotype 3) harbored plasmids sized approximately 47 and 44 megadaltons, respectively. no such plasmids were found in "apathogenic" strains of y. enterocolitica belonging to biotype 1. there was a positive correlation among the presence of plasmid, autoagglutination, and adherence to and toxicity for hep-2 cell cultures; all of these properties were lost by culturing at 37 degrees c in the absence of calcium. strains of ...19817287174
first isolation in puglia of human yersinia enterocolitica: two cases report. 19807459027
heat shock proteins in the human periodontal disease process.the production of hsp by periodontopathic gram-negative bacteria was examined by sds-page, two dimensional gel electrophoresis, and western blotting using monoclonal antibodies against hsps. strains of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, prevotella intermedia, prevotella nigrescens, prevotella melaninogenica, and treponema socranskii species produced hsp which reacted with anti-yersinia enterocolitica hsp 60 and/or mycobacterial 65-kda hsp monoclon ...19957565172
susceptibility to beta-lactam agents of yersinia enterocolitica biotype 4, serotype o3 isolated in various parts of the world.forty-eight human isolates of yersinia enterocolitica of biotype 4, serotype o3 from various parts of the world were examined for susceptibility to 13 beta-lactam agents. the intracellular beta-lactamases of each of the 48 strains were examined. isolates from europe, asia and brazil (phage type viii) or south africa and hungary (phage type ixa) produced both enzyme a and enzyme b, whereas isolates from new zealand and australia (phage type ixb) lacked the cephalosporinase enzyme b. among the sev ...19957608962
yersinia enterocolitica is a common cause of gastroenteritis in auckland.infections with yersinia enterocolitica are a significant cause of gastroenteritis in many countries, however, little information is available on the incidence of human disease in new zealand. a study was performed between january 1988 and december 1993 to investigate aspects of the epidemiology of yersiniosis in the auckland region including prevalence, age and sex distribution, strain definition and seasonal occurrence.19957637924
yersinia lipopolysaccharide is modified by human monocytes.reactive arthritis is usually self-limiting polyarthritis, which develops after certain gastrointestinal or urogenital tract infections, mostly in susceptible hla b27-positive individuals. in the pathogenesis of this arthritis, it is probably important that structures of the causative bacteria are found in the affected joints. the structure found in the synovial fluid phagocytes of the patients with reactive arthritis after yersinia, salmonella, and shigella infections has always been lipopolysa ...19937693597
role of yops in inhibition of phagocytosis and killing of opsonized yersinia enterocolitica by human granulocytes.the virulence plasmid of yersinia enterocolitica codes for the production of the outer membrane protein yada and the secretion of several proteins, called yops, which may play a role in the interaction between granulocytes and this bacterium. we investigated whether the expression of yada or the secretion of yops affected the phagocytosis and killing of opsonized y. enterocolitica by human granulocytes. the rates of phagocytosis and killing of y. enterocolitica by granulocytes in suspension in t ...19957790071
a distinct array of proinflammatory cytokines is expressed in human colon epithelial cells in response to bacterial invasion.pathogenic bacteria that penetrate the intestinal epithelial barrier stimulate an inflammatory response in the adjacent intestinal mucosa. the present studies asked whether colon epithelial cells can provide signals that are important for the initiation and amplification of an acute mucosal inflammatory response. infection of monolayers of human colon epithelial cell lines (t84, ht29, caco-2) with invasive strains of bacteria (salmonella dublin, shigella dysenteriae, yersinia enterocolitica, lis ...19957814646
genomic fingerprinting of yersinia enterocolitica species by degenerate oligonucleotide-primed polymerase chain reaction.a novel version of the polymerase chain reaction (pcr) termed degenerate oligonucleotide-primed pcr (dop-pcr) was used to fingerprint the bacterial genome of yersinia enterocolitica species. eight well-characterized reference strains of y. enterocolitica (ribotype, serotype, biotype and zymotype) were examined. optimal experimental conditions for reproducibility, sensitivity and specificity were obtained using a single dop-primer. all the strains gave distinct dop-pcr profiles composed of 12 to ...19947925230
a simple procedure to differentiate aila and ailna gene variants among human pathogenic yersinia enterocolitica strains.the identification and differentiation of the two variants of the ail gene, aila from the more virulent american serotypes (08, 013a, 13b, 018, 020, 021) and ailna from the less virulent non-american serotypes (03, 04, 05, 06, 09, 027 and 07, 8) was studied in a panel of 32 yersinia enterocolitica human pathogenic isolates. a 444 bp fragment corresponding to the ail gene was amplified using a pcr procedure in all tested strains. subsequent digestion of the pcr product by rsal and by haeiii endon ...19947969190
duration of carriage and transmission of yersinia enterocolitica biotype 4, serotype 0:3 in dogs.human infections with pathogenic strains of yersinia enterocolitica have been linked to contact with dogs excreting these microorganisms. this study examines the carriage and transmission of y. enterocolitica biotype 4, serotype 03 in dogs. fourteen 6-month-old cross-bred dogs were separated into 5 groups, 2 containing 4 dogs (i and ii) and the others 2 dogs (iii-v). each of the 4 dogs in group i and 2 of the dogs in group ii were inoculated orally with the test strain. bacteriological examinati ...19947995357
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