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comamonas testosteroni bacteremia in a patient with perforated acute appendicitis. short communication.comamonas testosteroni is an uncommon isolate in the clinical laboratory as a human pathogen. c. testosteroni most commonly emerges in abdominal pathologies especially in perforated appendicitis. in turkey we report first time a case of bacteremia due to this organism, in a 22-year-old man with perforated acute appendicitis. the organism was shown to be susceptible to routine antibiotics so it was easily eliminated even after having caused a bacteremia.200717896478
use of bioconversion for the preparation of [4-14c]-labeled 7 alpha- and 7 beta-hydroxylated derivatives of dehydroepiandrosterone and epiandrosterone.the 7 alpha- and 7 beta-hydroxylated derivatives of [4-14c]-dehydroepiandrosterone were prepared with use of the yeast-expressed human cytochrome p4507b1. epiandrosterone (epia), 5 alpha-androstane-3beta,17 beta-diol, and 5 alpha-androstane-3,17-dione were obtained after incubation of [4-14c]-5 alpha-dihydrotestosterone with escherichia coli-expressed (3beta,17 beta)-hydroxysteroid dehydrogenase from pseudomonas testosteroni. the 7 alpha- and 7 beta-hydroxylated derivatives of [4-14c]-epia produ ...200212441198
carbonyl reduction of metyrapone in human liver.carbonyl reduction was investigated in cytosolic and microsomal fractions of human liver using the ketone metyrapone as a substrate. the cytosolic enzyme has a stronger preference for nadph over nadh than the microsomal enzyme: the former shows only 14% of the nadph-supported activity while the latter exhibits 36% activity with nadh. barbitone and quercitrin, the classic inhibitors of carbonyl reductases, do not affect metyrapone reduction in either fraction. dicumarol and indomethacin, the spec ...19911722672
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