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specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction.development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ...19921400961
associations between microbial species in dental root canal infections.the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ...19921494447
dental bacterial plaques. nature and role in periodontal disease.antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ...19911890226
plaque and chronic inflammatory periodontal disease. a question of ecology.the nature of the relationship between dental plaque and chronic inflammatory periodontal disease (cipd) remains unclear, although there is no doubt that plaque is the direct cause. non-specific, specific and exogenous hypotheses have been proposed to explain plaque-host relationships. current evidence indicates that plaque is part of the natural human microflora, one of many such in nature, and that disruption of oral microbial ecology, due primarily to diet texture changes, leads to gingivitis ...19902212083
effect of campylobacter rectus lps on plasminogen activator-plasmin system in human gingival fibroblast cells.the plasminogen activator (pa)-plasmin system is implicated in the degradation of the extracellular matrix in inflammation through activation of metalloproteases and prekallikrein. we examined the activation of the pa-plasmin system in human gingival fibroblast cells (gin-1 cells) following treatment with lipopolysaccharide (lps) from campylobacter rectus, which is frequently detected at sites of periodontal disease. the c. rectus lps stimulated the plasmin activity in the conditioned medium of ...19957776154
production of inflammatory mediators and cytokines by human gingival fibroblasts following bacterial challenge.bacteria can indirectly affect the course of periodontal diseases by activating host cells to produce and release inflammatory mediators and cytokines. these mediators and cytokines manifest potent proinflammatory and catabolic activity and may play key roles in local amplification of the immune response as well as in periodontal tissue breakdown. this study tested the effect of actinobacillus actinomycetemcomitans (aa) and campylobacter rectus (cr) challenge on pge2, il-1 beta, il-6 and il-8 pr ...19968708945
degradation of lactoferrin by periodontitis-associated bacteria.the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ...19968961558
role for the s-layer of campylobacter rectus atcc33238 in complement mediated killing and phagocytic killing by leukocytes from guinea pig and human peripheral blood.the role of the s-layer of campylobacter rectus atcc 33238 in complement-mediated killing and interaction with leukocytes of the intraperitoneal cavity from guinea pigs and human peripheral blood was studied in vitro.19979467352
the s-layer protein from campylobacter rectus: sequence determination and function of the recombinant protein.the gene encoding the crystalline surface layer (s-layer) protein from campylobacter rectus, designated slp, was sequenced and the recombinant gene product was expressed in escherichia coli. the gene consisted of 4086 nucleotides encoding a protein with 1361 amino acids. the n-terminal amino acid sequence revealed that slp did not contain a signal sequence, but that the initial methionine residue was processed. the deduced amino acid sequence displayed some common characteristic features of s-la ...19989770285
the groel-like protein from campylobacter rectus: immunological characterization and interleukin-6 and -8 induction in human gingival fibroblast.the native groel-like protein was purified from campylobacter rectus, a putative periodontal pathogen, by affinity chromatography on atp-agarose followed by high performance liquid chromatography on superose 6. the purified 64-kda protein (denatured form of groel-like protein) was immunoreactive by sds-page and western immunoblotting with the monoclonal antibody directed against heat shock protein 60 of human origin. the native groel-like protein stimulated both interleukin-6 (il-6) and il-8 sec ...19989785445
enhancement of lipopolysaccharide-stimulated pge2 and il-1beta production in gingival fibroblast cells from old rats.the effect of aging on gingival fibroblasts in response to bacterial infection was studied. rat gingival fibroblast (rgf) cells were cultured from gingival tissue removed from young (6 weeks old) and old (20 months old) rats. both types of rgf cells were challenged with lipopolysaccharide (lps) from the periodontal pathogen campylobacter rectus. the levels of prostaglandin e2 (pge2) and interleukin 1beta (il-1beta) released into the cultured medium were measured by a specific radioimmunoassay. l ...199910433392
human serum antibody responses to oral microorganisms. iv. correlation with homologous infection.recent microbiological studies of periodontal disease in humans have supported the concept of a specific bacterial etiology. while individual agents have not been unequivocally identified, numerous gram-negative members of the subgingival microflora have been implicated. in addition, elevations in systemic antibody responses have been consistent with certain oral microorganisms being involved in an infectious process associated with the disease. this report delineates the relationship between el ...198710870468
antimicrobial activity of silver nitrate against periodontal pathogens.metal ions were evaluated as potential antimicrobial agents suitable for local delivery in the oral cavity for the treatment of periodontitis. silver nitrate, copper chloride, and zinc chloride were tested for antimicrobial activity in in vitro killing assays conducted in phosphate buffered saline with a series of oral bacteria including gram-negative periodontal pathogens and gram-positive streptococci. copper and zinc salts failed to exhibit strong and consistent activity against periodontal p ...200111327077
microbiological, immunological and genetic factors in family members with periodontitis as a manifestation of systemic disease, associated with hematological disorders.the microflora, immunological profiles of host defence functions, and human leukocyte antigen (hla) findings are reported for a mother, son and daughter who were diagnosed as having 'periodontitis as a manifestation of systemic diseases, associated with hematological disorders'. examinations were made of the bacterial flora from the periodontal pocket, neutrophil chemotaxis, neutrophil phagocytosis, and the genotypes (dqb1) and serotypes (dr locus) of hla class ii antigens. phenotypic analyses o ...200212200976
herpesviral-bacterial interrelationships in aggressive periodontitis.recent findings have begun to provide a basis for a causal link between herpesviruses and aggressive periodontitis. one theory is that herpesviruses cooperate with specific bacteria in the etiopathogenesis of the disease. this study examined whether the presence of herpesviruses [human cytomegalovirus (hcmv), epstein-barr virus (ebv) type 1, herpes simplex virus (hsv) type 1 and 2] is associated with the presence of putative pathogenic bacteria (porphyromonas gingivalis, prevotella intermedia, t ...200415206912
campylobacter rectus mediates growth restriction in pregnant mice.recent studies have suggested that subclinical infection may be an important cause of low birth weight. campylobacters are important human pathogens, causing septicemia and occasionally abortion, premature labor, or severe perinatal infection. the potential role of oral species of campylobacter in mediating adverse pregnancy outcomes in animal models has not yet been determined. our objective was to determine the effects of campylobacter rectus (c. rectus) infection on pregnancy outcomes in a mo ...200515857095
campylobacter surface-layers (s-layers) and immune evasion.many pathogenic bacteria have evolved mechanisms for evading host immune systems. one evasion mechanism is manifest by the surface layer (s-layer), a paracrystalline protein structure composed of s-layer proteins (slps). the s-layer, possessed by 2 campylobacter species (c. fetus and c. rectus), is external to the bacterial outer membrane and can have multiple functions in immune avoidance. c. fetus is a pathogen of ungulates and immunocompromised humans, in whom it causes disseminated bloodstre ...200216013216
streptococci dominate the diverse flora within buccal cells.previously, we reported that intracellular actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. in this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify fusobacterium nucleatum, prevotella intermedia, oral campylobacter species, eikenella corrodens, treponema denticola, gemella haemo ...200516304448
a model of spontaneous periodontitis in the miniature goat.the pathogenesis and progression of periodontal disease have been extensively studied through the use of animal models. however, no animal model has yet been established that is precisely similar to periodontitis in humans. in the present study, we examined the use of shiba goats as a model for spontaneous periodontitis.200616671878
demographic, clinical, and microbial aspects of chronic and aggressive periodontitis in colombia: a multicenter study.the microbial profile of periodontal disease varies among different human populations. this study evaluated the demographic, clinical, and microbiologic aspects of periodontitis in a multigeographic sample in colombia.200717397309
herpesviral-bacterial synergy in the pathogenesis of human periodontitis.periodontitis is an infectious disease, but the specific mechanisms by which tooth-supportive tissues are lost remain obscure. this article proposes an infectious disease model for periodontitis in which herpesviral-bacterial interactions assume a major etiopathogenic role.200717471038
granulocyte chemotactic protein 2 (gcp-2/cxcl6) complements interleukin-8 in periodontal disease.mucosal inflammatory responses are orchestrated largely by pro-inflammatory chemokines. the chemokine granulocyte chemotactic protein 2 (cxcl6) is involved in neutrophil recruitment and migration. previous studies have shown that granulocyte chemotactic protein 2 is up-regulated during mucosal inflammation (e.g. in inflammatory bowel disease), similarly to the functionally and structurally related chemokine interleukin-8. nevertheless, unlike interleukin-8, a role of granulocyte chemotactic prot ...200918842116
analysis of matrix metalloproteinase (mmp-8 and mmp-2) activity in gingival crevicular fluid from children with down's syndrome.high levels of colonization by periodontopathic bacteria and a high prevalence of chronic inflammatory periodontal disease have been reported in children with down's syndrome. matrix metalloproteinases (mmps) are mediators of extracellular matrix degradation and remodelling, and are deeply involved in the course of periodontal disease. to clarify the relationship between down's syndrome and periodontitis, we investigated levels of mmp-2 and mmp-8 in gingival crevicular fluid (gcf) and detection ...201019778333
profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time pcr.qualitative and quantitative changes of the subgingival plaque biofilm microflora in periodontal pockets are thought to be associated with the development and progression of periodontitis. the aims of the present study were to quantify the proportions of nine periodontitis-associated bacterial species and four streptococcus species in subgingival plaque, and to evaluate their relationship with periodontitis quantitatively.201020337892
clinical periodontal and microbiologic parameters in patients with crohn's disease with consideration of the card15 genotype.crohn's disease (cd) was reported to have oral manifestations. however, data on periodontal parameters and oral microbiology in cd are rare. recent studies showed associations of variants in the caspase recruitment domain (card)15 gene with cd that are involved in the immune response toward bacterial products. our aim is to investigate the periodontal status and prevalence of periodontal pathogens in patients with cd under consideration of the card15 polymorphism.201020373538
molecular detection of human periodontal pathogens in oral swab specimens from dogs in japan.periodontal diseases are known to be major diseases in humans, and are also common in dogs. the purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. the distribution of an animal gingival organism porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including porphyromonas gingivalis, treponema denticola, tannerella forsythia, capnocytophaga ochracea, capnocyt ...201121916371
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