specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction.development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ...19921400961
illness associated with campylobacter laridis, a newly recognized campylobacter species.campylobacter laridis, a recently described thermophilic campylobacter species found principally in seagulls, has not previously been linked to illness in humans. six clinical isolates of this species were referred to the national campylobacter reference laboratory in 1982 and 1983. each isolate was confirmed by biochemical characterization and by dna relatedness studies. the six isolates were obtained during an illness: enteritis in four, severe crampy abdominal pain in one, and terminal bacter ...19853972989
a pcr-dgge method for detection and identification of campylobacter, helicobacter, arcobacter and related epsilobacteria and its application to saliva samples from humans and domestic develop a pcr-denaturing gradient gel electrophoresis (pcr-dgge) method for the detection and identification of campylobacter, helicobacter and arcobacter species (epsilobacteria) in clinical samples and evaluate its efficacy on saliva samples from humans and domestic pets.200717916160
development of a novel triplex pcr assay for the detection and differentiation of thermophilic species of campylobacter using 16s-23s rdna internal transcribed spacer (its) region.campylobacter species are significantly implicated in human gastrointestinal infections. of 20 species of campylobacter, c. jejuni, c. coli and c. lari have been considered as the most important causative agents of human infections. in order to better understand the occurrence and epidemiology of these thermophilic campylobacter species, an improved and rapid detection method is warranted. a novel triplex polymerase chain reaction (pcr) assay was developed based on the variable 16s-23s rdna inte ...200718045440
comparative performance of different pcr assays for the identification of campylobacter jejuni and campylobacter coli.the sensitivity and specificity of 7 pcr assays described for the identification of campylobacter jejuni and campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of c. jejuni, c. coli, campylobacter lari and related campylobacter, helicobacter and arcobacter species. based on a preliminary evaluation, one multiplex test was excluded from further evaluation. the various assays differed considerably in sensitivity and specificit ...200818272350
prevalence of thermotolerant campylobacter in partridges (perdix perdix).to estimate the prevalence of thermotolerant campylobacter spp. in commercially reared partridges (perdix perdix) in southern italy.200919552770
prevalence of campylobacter spp. in raw retail poultry on sale in northern ireland.a year-long survey of fresh, retail poultry products on sale in northern ireland was undertaken to define the prevalence of campylobacter spp. by using protocols based on iso (standard) 10272-1:2006. incubation at 37 and 42 degrees c was undertaken to increase the diversity of isolates obtained. overall, 652 isolates were identified as campylobacter spp. by using pcr and amplified fragment length polymorphic typing. phenotyping wrongly identified 21% of isolates. prevalences of campylobacter fou ...200919777882
eleutherine americana: a candidate for the control of campylobacter species.the antibacterial activity of ethanolic extracts of selected thai medicinal plants (rhodomyrtus tomentosa (aiton) hassk., quercus infectoria g. olivier, and eleutherine americana merr.) against campylobacter spp. was investigated. sixty-five campylobacter, including 39 isolates from humans and 26 isolates from chicken samples, were tested. reference campylobacter spp. that are commonly encountered in gastroenteritis were included. the ethanolic extract of e. americana demonstrated good antibacte ...201121406364
emerging dynamics of human campylobacteriosis in southern ireland.infections with campylobacter spp. pose a significant health burden worldwide. the significance of campylobacter jejuni/campylobacter coli infection is well appreciated but the contribution of non-c. jejuni/c. coli spp. to human gastroenteritis is largely unknown. in this study, we employed a two-tiered molecular study on 7194 patient faecal samples received by the microbiology department in cork university hospital during 2009. the first step, using entericbio(®) (serosep), a multiplex pcr syst ...201122077228
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