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campylobacter enteritis associated with canine infection.campylobacter fetus ss. jejuni has recently been recognised as a human enteric pathogen. five cases of campylobacter enteritis are described. all five patients had abdominal pain and diarrhoea. two of the five patients had bloody diarrhoea and relapses. all the patients had been in contact with young dogs which had had diarrhoea. campylobacter fetus ss. jejuni was isolated from these dogs or from their litter-mates. canine infection with campylobacter fetus ss. jejuni may be an important source ...197882001
campylobacter fetus infections in children.there has been a gradual accumulation of reported campylobacter fetus human infections since the first description in 1947. the taxonomy of these fastidious and morphologically confusing organisms has been recently revised and, in the past few years, through the use of selective culture medium for stool isolation c fetus has been implicated as a frequent paghogen in children. there are three relatively distinct patterns of human c fetus infection. the first, most frequent, pattern of disease is ...1979390487
campylobacter enteritis associated with unpasteurized milk.campylobacter fetus ss. jejuni has recently been recognized as a human enteric pathogen. laboratory isolation has been hindered by its fastidious nature. methods for recovery of this organism from stool culture and a specific serologic test are described. an outbreak is reported in which three members of the same family became simultaneously ill with fever, severe abdominal cramps and diarrhea. c. fetus ss. jejuni was recovered from stool specimens from all three. a fourfold increase in serum im ...1979573969
improved media for growth and aerotolerance of campylobacter fetus.the microaerophilic nature of campylobacter fetus has complicated its recovery from human and animal sources. in this study, modifications of brucella agar and broth were tested for enhancement of growth and aerotolerance of 64 strains of c. fetus, representing each subspecies. brucella agar supplemented with 0.025% each feso4 7h2o, sodium metabisulfite, and sodium pyruvate, supported growth of 98, 77, and 63% of the strains at 6% o2, 17% o2, and 21% o2, respectively. unsupplemented brucella aga ...1978670386
campylobacteriosis in man: pathogenic mechanisms and review of 91 bloodstream infections.five patients with campylobacter fetus(previously called "vibrio fetus") bacteremia are presented with enteric symptoms in four patients, a self-limited course in three, and with possible nosocomial infection in one patient who had disseminated malignancy. the clinical syndromes of 91 bacteremic patients with campylobacteriosis and c. fetus taxonomy and pathogenicity are reviewed. studies of potential pathogenic mechanisms in enteric infections failed to reveal the production of either heat-stab ...1978707518
campylobacter fetus associated with pulmonary abscess and empyema.an unusual case of community-acquired necrotizing aspiration pneumonia with pulmonary abscess and empyema associated with campylobacter fetus (formerly vibrio fetus) in mixed culture is reported. this microaerophilic gram-negative curved bacillus has been increasingly associated with human disease. unlike most pathogens associated with community-acquired aspiration pneumonia, this organism is usually resistant to penicillin. infection associated with this organism is characteristically relapsing ...1977830489
campylobacter enteritis: a "new" disease.by selective culture campylobacters (c jejuni and c coli) were isolated from the faeces of 57 (7-1%) out of 803 unselected patients with diarrhoea; none were isolated from 194 people who had not got diarrhoea. specific agglutinins were found in the sera of 31 out of 38 patients with campylobacter enteritis and 10 of them had a rising titre. half the patients were aged 15 to 44 years, but the incidence was highest in young children. all the patients with campylobacters had a distinctive clinical ...1977871765
campylobacter fetus septicemia with concurrent salpingitis.campylobacter fetus was isolated from the blood of a nonpregnant young woman who had a clinical diagnosis of salpingitis. a description of the isolate of c. fetus is given. an increasing awareness of human infections and capability of growing this microaerophilic organism should contribute to our understanding of the incidence and virulence of this bacterium.1977886009
specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction.development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ...19921400961
pyogenic arthritis of the hip due to campylobacter fetus--a case report.septic arthritis of the hip caused by campylobacter fetus subsp. fetusis very rare. the authoris isolated c. fetus subsp. fetus from a specimen of the left hip. the patient was a 53-year old man with a history of heavy drinking, diabetes, and chronic hepatitis, and had been suffering from avascular necrosis of both femoral heads. it was considered that the organism invaded already damaged tissue of the joint. the patient was treated with intravenous antibiotics and later received successful tota ...19921502834
[campylobacter fetus spondylodiscitis].the case of a sixty-two year old man with campylobacter fetus spondylodiscitis is reported. despite of adequate antibiotherapy, the outcome was bad and death occurred. three other cases of campylobacter fetus spondylodiscitis are reported in the literature. tomodensitometry disclosed a paravertebral abscess with gas, that make our observation original.19921579850
campylobacter fetus infection of abdominal aortic aneurysm.a 61-year-old man with campylobacter fetus infection of an abdominal aortic aneurysm treated surgically is presented herein, the fifth survival case reported in the literature. fever and back pain preceded the enlargement of atherosclerotic abdominal aortic aneurysm. the patient tolerated satisfactorily total excision of the aneurysm followed by axillo-femoral prosthetic bypass. antibiotic therapy consisted of intravenous infusion of fosfomycin and gentamicin and oral administration of minocycli ...19902262502
protection of breast-fed infants against campylobacter diarrhea by antibodies in human milk.to investigate the role of breast-feeding in preventing diarrhea caused by campylobacter jejuni, we followed 98 mexican children prospectively for 2 years beginning at their birth. attack rates of diarrhea in children less than 6 months of age who were not fed human milk were 2.3 times greater than those in children of the same age who were fed human milk. breast-fed children remained free of diarrhea for a longer time than non-breast-fed children (p less than 0.0005). the diarrhea attack rate c ...19902329419
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in austria to erythromycin and ciprofloxacin.more than 200 strains of campylobacter (c.) jejuni/coli isolated in 1985 and 1987/88 from human fecal specimens were tested for their susceptibility to erythromycin and ciprofloxacin. their mic90 as assessed by agar dilution tests was 2.0 and 0.5 mg/l, respectively. thus, all strains were regarded as susceptible to ciprofloxacin. with 2 out of 55 strains of c. coli the mic of erythromycin was 8.0 mg/l. therefore, only 3.6% of the c. coli strains were resistant to erythromycin. all 209 strains of ...19902360966
campylobacter fetus subspecies fetus infection of an abdominal aneurysm.we report a case of aneurysm of the abdominal aorta with spontaneous rupture in a 64-year-old man with emphysema and chronic alcoholism. campylobacter fetus subspecies fetus was repeatedly recovered from the blood and from specimens of the excised aneurysm. the patient died in spite of early diagnosis, intravenous erythromycin and surgical intervention. the bacteriology and epidemiology of campylobacteriosis in man is briefly discussed and eight other published cases of aneurysm, infected with t ...19892678860
diversity of serotypes of campylobacter jejuni and campylobacter coli isolated in laboratory animals.one hundred nineteen isolates of campylobacter jejuni and campylobacter coli from nine laboratory animal species were serotyped using antisera to 20 penner serotypes commonly isolated from cases of human enteric infections. although c. jejuni and c. coli were isolated from laboratory animals with diarrhea, the majority were cultured from asymptomatic animals (81%). seven of twenty-two isolates from animals with diarrhea were serotype 4 (32%) and three were serotype 1 (14%). sixty-one of the 119 ...19892724920
identification and characterization of two campylobacter jejuni adhesins for cellular and mucous substrates.campylobacter jejuni is able to colonize the human intestinal mucosa and cause disease. for this reason, it was important to investigate mechanisms by which c. jejuni adheres to epithelial cells and intestinal mucus gel. all strains of c. jejuni used were able to adhere to int 407 epithelial cells and mucus, but high adherence to one substrate did not necessarily indicate comparable adherence to the other. the adherence of c. jejuni to cells was inhibited partially by treating the bacterial cell ...19862873103
restriction endonuclease digest patterns of chromosomal dna from nitrate-negative campylobacter jejuni-like organisms.high molecular weight chromosomal dna was isolated from ten nitrate-negative campylobacters of human origin (gastric biopsies and faecal specimens). the dna was digested with various restriction endonucleases and the patterns obtained were compared with those of campylobacter coli, c. fetus subsp. fetus, c. jejuni and c. laridis reference strains. electrophoresis of hae iii digests of dna in 0.5% agarose gave excellent patterns which comprised at least 25 well-resolved bands with fragment sizes ...19852876913
in vitro susceptibility of campylobacter jejuni to 27 antimicrobial agents and various combinations of beta-lactams with clavulanic acid or sulbactam.the in vitro susceptibility of human isolates of campylobacter jejuni was investigated with 27 antibiotics and 8 combinations of beta-lactams with clavulanic acid or sulbactam. ansamycin, the new quinolines, erythromycin, and cefpirome were the most active drugs against c. jejuni; amoxicillin, ampicillin, cefotaxime, and ceftazidime 90% of the isolates, greater than or equal to 50 mg/liter). the activity of various beta-lactams was unchanged by the addition of clavulanic acid or sulbactam.19852994557
campylobacters in man and the environment in hull and east yorkshire.campylobacter organisms isolated from water samples taken weekly from ponds and land-drains in the city of hull were compared with isolates from humans. of 314 campylobacter organisms isolated from patients, 237 (75.5%) of the strains were identified as typical campylobacter jejuni, whilst of 125 identified strains isolated from the water samples, 85 (68%) resembled c. jejuni in most respects but were hippurate hydrolysis negative by the hwang and ederer method. the ponds and land drains in the ...19883181312
incidence of campylobacter jejuni in processed egg products.egg samples were obtained from two commercial egg-processing facilities to determine if campylobacter jejuni could be isolated from the raw product or from further-processed egg products intended for human consumption. no c. jejuni were detected in any of the egg or egg-product samples or from water samples collected from the overflow of the egg washer. campylobacter jejuni was recovered from all control samples, which had been inoculated at the plants. these findings suggest that properly proce ...19883194336
campylobacter jejuni infections in gnotobiotic pigs.at 3 days of age, 12 gnotobiotic pigs were inoculated orally with broth cultures of campylobacter jejuni. one pig was euthanatized and evaluated each day for 12 days. in the cecum and colon, there was diffuse edema, neutrophilic infiltration, and sloughing of epithelial cells from the mucosa on postinoculation days (pid) 2 through 5. dysplastic colonic crypt epithelial cells were observed in the submucosa of the colon on pid 5 through 12. curved, rod-shaped bacteria were detected on the surface ...19883377304
laboratory and clinical evaluation of isolation media for campylobacter jejuni.six selective isolation media were evaluated for their ability to support the growth of campylobacter jejuni. colony counts of 70 isolated strains of c. jejuni and recovery studies on these strains in simulated positive feces samples demonstrated that bolton and hutchinson' charcoal, cefoperazone, deoxycholate agar and karmali's charcoal-based selective medium produced the highest recovery rates with the greatest suppression of other fecal flora. c. jejuni colonies were more easily recognized on ...19873429621
variations in the virulence, for pregnant guinea pigs, of campylobacters isolated from man.pregnant guinea pigs were used to compare the virulence of four human isolates of campylobacter fetus ss. fetus and four of c. jejuni on the basis of their ability to cause abortion and bacteraemia. of the four strains of c. fetus ss. fetus two produced abortion readily after intramuscular injection. the four c. jejuni isolates were, however, of comparatively low virulence and no differences between them were demonstrated. some of the isolates differed in their ability to survive in vitro in hum ...19873560189
a blood-free medium for isolation of thermophilic campylobacter species.a blood-free medium for the recovery of thermophilic campylobacter was compared with butzler medium virion during a one-year study using 2,893 human feces samples. ninety campylobacter strains (3.1%) were isolated after incubation for 48 h at 42 degrees c in a candle jar atmosphere. three strains of campylobacter jejuni were isolated on the blood-free medium only and one on butzler medium virion only. fecal flora was equally well inhibited on both media except for gram-positive organisms, which ...19873595583
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in denmark to fourteen antimicrobial agents.the in vitro susceptibility of 95 recent danish human clinical isolates of campylobacter jejuni and campylobacter coli to 14 antimicrobial agents was determined by an agar dilution technique. c. jejuni and c. coli were considered together. doxycycline, ciprofloxacin, gentamicin, clindamycin, nitrofurantoin and erythromycin were the most active drugs. 2% of the strains tested were resistant to erythromycin (mic greater than 3.1 micrograms/ml). therefore susceptibility testing is suggested if anti ...19873630712
naturally occurring auxotrophs of campylobacter jejuni and campylobacter coli.the nutritional requirements for 439 campylobacter jejuni isolates and 46 campylobacter coli isolates were determined by using a previously described chemically defined medium, campylobacter defined medium. with this medium, 45% of both human and nonhuman c. jejuni isolates demonstrated auxotrophic requirements. none of the 46 c. coli isolates studied demonstrated requirements for amino acids on campylobacter defined medium. the most common auxotrophic requirement among c. jejuni isolates was fo ...19873654939
counterimmunoelectrophoresis compared with complement fixation and passive haemagglutination tests in the evaluation of the immune response in campylobacter infections.the immune response was studied in 238 human patients with campylobacter jejuni/coli (cjc)-infections in rotterdam by the counterimmunoelectrophoresis (cie) test, a commercial complement fixation test (cft) and the passive haemagglutination test (ha). antibodies became detectable in the three tests around 4 days after the onset of complaints. between the 7th and the 20th days after onset of illness 79%, 80% and 53% of the patients demonstrated antibodies by the cie, the cft and the ha, respectiv ...19873662488
colonization and infection of athymic and euthymic germfree mice by campylobacter jejuni and campylobacter fetus subsp. fetus.human clinical strains of campylobacter jejuni and campylobacter fetus subsp. fetus colonized the gastrointestinal tracts of both athymic (nu/nu) and euthymic (+/nu) germfree mice (balb/c). viable campylobacter spp. (10(9) to 10(10) cfu/g [dry weight] of cecum and colon contents) were isolated on day 3 after oral challenge, and similar large numbers of viable cells were evident at several intervals during a 10-month experiment. the stomachs and upper small intestines of nu/nu and +/nu mice that ...19863733222
serotyping of campylobacter fetus subsp. fetus and c. fetus subsp. venerealis by passive hemagglutination technique based on soluble autoclaved antigens.antigens for sensitization of sheep erythrocytes were released from c. fetus subsp. fetus and c. fetus subsp. venerealis suspensions by autoclaving in alkaline and neutral saline and by boiling in alkaline but not in neutral saline. serotyping with rabbit antisera showed the same two serotypes of o-antigens, a and b, as shown by tube agglutination of heated bacteria. all c. fetus subsp. venerealis strains had the a antigen. the c. fetus subsp. fetus strains tested had either the a or b antigen. ...19863751579
phagocytosis of campylobacter jejuni and its intracellular survival in mononuclear phagocytes.in vitro phagocytosis and intracellular survival of campylobacter jejuni strain 2964 in mononuclear phagocytes were studied. the following three types of mononuclear phagocytes were used: a j774g8 peritoneal macrophage line derived from balb/c mice, resident balb/c peritoneal macrophages, and human peripheral blood monocytes. when c. jejuni and mononuclear phagocytes were combined at a ratio of 75:1, light microscopy, fluorescent microscopy, and electron microscopy all indicated that c. jejuni c ...19853988342
infected aortic aneurysms.salmonella organisms have been responsible for half of the reported cases of infected aortic aneurysms, although other bacteria have been incriminated. this is the second reported case in which campylobacter fetus subspecies fetus apparently infected an inflammatory aortic aneurysm. in this 70-year-old man the organism was isolated and treated prior to resection of the aneurysm and inline grafting. specific antibiotic therapy was continued for 8 weeks postoperatively. examination and ct scans ob ...19854009843
biotypes and serotypes of thermophilic campylobacters isolated from cattle, sheep and pig offal and other red meats.in this study we examined 730 faecal samples of offal (mainly liver), mince-meat and sausage meat collected from abattoirs and retail butchers' shops for campylobacters. campylobacter jejuni or c. coli were isolated from 30.6, 10.5 and 6% of sheep, cattle and pig offal samples respectively. specimens collected from abattoirs were, in general, more often contaminated than material obtained from retail butchers' shops. only 1.4% of minced meats and sausage meats contained campylobacters. most isol ...19854020105
human infection with vibrio fetus.vibrio fetus, a slowly growing, microaerophilic, gram-negative rod which causes infectious abortion in cattle, occasionally induces disease in man. since persons receiving immunosuppressive agents are among those most susceptible to this infection, recognition of the disease may become increasingly important as transplantation operations increase. vibrio infections may produce a spectrum of acute and chronic illnesses or may be asymptomatic. vibriosis may develop at any age but it seems most dev ...19744816399
raw liver as a possible source of vibrio fetus septicemia in man. 19715104711
vibrio fetus infection in man. i. ten new cases and some epidemiologic observations. 19705421893
human infection with vibrio fetus. 19675596135
[2 human cases of vibrio fetus infection]. 19675599984
antral mucosal diaphragm. clinical and roentgen characteristics, with first reported case of vibrio fetus in human bile. 19665930786
respiratory physiology and energy conservation efficiency of campylobacter jejuni.a study of the electron transport chain of the human intestinal pathogen campylobacter jejuni revealed a rich complement of b- and c-type cytochromes. two c-type cytochromes were partially purified: one, possibly an oxidase, bound carbon monoxide whereas the other, of high potential was unreactive with carbon monoxide. respiratory activities determined with membrane vesicles were 50- to 100-fold higher with formate and hydrogen than with succinate, lactate, malate, or nadh as substrates. evidenc ...19826277867
investigation of a campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis.fifty campylobacter jejuni isolates, including 29 from humans associated with an outbreak of enteritis, 20 from cattle, and 1 from a milk source, were serotyped on the basis of extractable thermostable antigens and examined by bacterial chromosomal restriction endonuclease digest analysis. serotyping showed specific differences between the human isolates and the milk isolates, but each of these generally, although not consistently, reacted with 4 of the 42 c. jejuni typing antisera. restriction ...19846325487
an acid extract as a common antigen in campylobacter coli and campylobacter jejuni strains.an acid extract prepared from a single strain of campylobacter jejuni reacted with rabbit antisera against 21 strains of c. jejuni and campylobacter coli. the immunogen is resistant to formalin and to exposure to 100 degrees c for 30 min. the antigenic extract may have practical applications in human serology.19836343426
campylobacter jejuni outer membrane proteins are antigenic for humans.all campylobacter jejuni strains have a major outer membrane protein (omp) that migrates between a molecular weight of 41,000 (41k) and 45k and represents more than 50% of protein present, plus several more minor bands. using 125i-radiolabeled c. jejuni cells in a radioimmunoprecipitation procedure to assess whether the omps were antigenic, we studied serum from rabbits immunized with c. jejuni cells, from humans convalescent after c. jejuni infection, and from appropriate controls. in this assa ...19846365789
intestinal carriage of campylobacter jejuni and salmonella by chicken flocks at slaughter.campylobacter jejuni were isolated in large numbers from the majority of birds sampled in colonic swabs from 28 of 60 flocks at slaughter. by contrast only small numbers of birds from 11 of the same 60 flocks yielded salmonella enteritidis serotypes. three c. jejuni isolates from each flock were serotyped on the basis of their heat-stable antigens, using antisera prepared against 16 serotypes common in campylobacter diarrhea in man. the majority (72 of 83) of the chicken isolates could be seroty ...19846383579
[campylobacter fetus subsp. jejuni: etiological, epidemiological and preventive aspects].the authors, after a brief introduction, examine on the basis of the most recent literature, the taxonomy and the microbiological features of campylobacter fetus subsp. jejuni, giving a detailed description of the chemical composition, of the biochemical, physiological and nutritional characters, of the antigenic composition and of the cultural methods. later on, they state the problems arising from the epidemiology of the infections caused by this germ either in man or in animals, examining the ...19846536547
the effect of storage in liquid nitrogen on the recovery of human dental plaque bacteria.centrifuged deposits of various individual bacteria from type-culture collections and dental plaque samples were immersed in liquid nitrogen for periods of up to 2 years. all the organisms were recovered after storage in liquid nitrogen but fusobacterium nucleatum (atcc 10953) and campylobacter fetus (nctc 10354) consistently showed a reduction in the number of colony-forming units after freezing. the main effect on bacterial proportions in plaque samples was a reduction in aerobic gram-negative ...19846596040
enhanced isolation of campylobacter jejuni by cold enrichment in campy-thio broth.isolation of campylobacter jejuni from human feces by direct inoculation to campy-bap (scott laboratories, inc., fiskeville, r.i.) was compared with isolation after overnight enrichment at 4 degrees c in campy-thio broth followed by subculture to campy-bap. of 54 positive specimens, 19 were positive only after enrichment, and 5 were positive only on the direct plate. among 36 positive patients, 10 were detected by enrichment only and 2 by direct plating only. laboratories using campy-bap should ...19836630456
simple adult rabbit model for campylobacter jejuni enteritis.we tested the usefulness of the removable intestinal tie adult rabbit diarrhea model to establish campylobacter jejuni infection in rabbits. the procedure involved ligation of the cecum, placement of a slip knot at the terminal ileum, and injection of the test inoculum into the mid-small bowel. the ends of the slip knot were externalized, and the tie was released 4 h later. fifty-five rabbits received c. jejuni, and 16 received uninoculated medium as controls. daily rectal swabs were positive fo ...19836642664
pathogenicity of campylobacter jejuni and campylobacter coli strains in the pregnant guinea pig model.pathogenicity of 17 campylobacter isolates for pregnant guinea pigs was investigated. of 14 isolates, 12 (86%) produced rates of abortion ranging from 13% to 87%. two isolates did not produce abortion. reference strains of c fetus subsp venerealis produced abortion in 60% to 87% and c fetus subsp fetus produced abortion in 60% of the guinea pigs. inoculated organisms were recovered from uterus, blood, liver, kidney, spleen, and gallbladder of the guinea pigs at rates as high as 83% for 2 ovine i ...19836650962
in vitro susceptibilities of 40 campylobacter fetus subsp. jejuni strains to niridazole and metronidazole.the activities of niridazole and metronidazole were compared by an agar dilution method against 40 strains of campylobacter fetus subsp. jejuni of human origin. niridazole had a markedly higher activity than metronidazole.19846703680
[campylobacter jejuni gastroenteritis].acute gastroenteritis is a major cause of hospital admission with attendant morbidity and occasional mortality. campylobacter jejuni recently has been recognized as a common cause of acute bacterial gastroenteritis in children and adults. its frequency of isolation is comparable to and in many studies exceeds that of salmonella isolations from diarrheal stools of hospitalized patients. medical workers in many parts of the world confirmed that campylobacter jejuni was found more commonly in feces ...19846748977
passive hemagglutination technique for serotyping campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens.antigenic materials were extracted from campylobacter fetus subsp. jejuni strains by heating bacterial suspensions in saline at 100 degrees c and by exposure to ethylenediaminetetraacetic acid. the antigens were heat stable at 100 degrees c, capable of sensitizing sheep erythrocytes for agglutination in antisera, and able to elicit production of specific antibody in rabbits; they occurred with different immunological specificities in 23 strains. antisera against the 23 strains could be used for ...19806796598
survival of campylobacter fetus subsp. jejuni in biological milieus.to provide new information on the epidemiology and pathophysiology of human infection with campylobacter fetus subsp. jejuni, we studied its survival in several milieus. standard inocula of organisms were placed in hydrochloric acid, human bile and urine, bovine milk, or stream water and kept at 4, 25, or 37 degrees c; viable organisms were then counted. stools from humans infected with campylobacter were similarly tested. survival in acid was ph and time dependent, with 7-log kill within 5 min ...19806892819
isolation of campylobacter fetus subsp. jejuni from human fecal specimens by incubation at 35 and 42 degrees c.campylobacter strains were isolated from human stool specimens that were cultured on modified skirrow agar and incubated at 35 and 42 degrees c. incubation at 42 degrees c resulted in more isolates, increased colony numbers, and earlier isolation than was found at 35 degrees c.19827119106
hippurate hydrolysis by and triphenyltetrazolium tolerance of campylobacter fetus.a rapid test of hippurate hydrolysis and a test of tolerance to triphenyltetrazolium chloride (ttc) were studied in 315 strains of campylobacter fetus subsp. jejuni to determine their usefulness for biotyping this organism and for distinguishing it from c. fetus subsp. intestinalis. of the 315 strains tested, 84% hydrolyzed hippurate and 97% were resistant to ttc. ability to hydrolyze hippurate was seen in 99% of 155 human isolates, 75% of 60 avian isolates, 100% of 41 cattle and dog isolates, 8 ...19827186902
intestinal colonization of neonatal animals by campylobacter fetus subsp. jejuni.neonatal mice (2.3 to 2.8 g) were inoculated intragastrically with different human isolates of campylobacter fetus subsp. jejuni. at weekly intervals thereafter, mice were sacrificed and dilution plate counts were performed on segments of the gastrointestinal tract. mice were uniformly colonized by some strains for 2 weeks, whereas other strains were being cleared at that time. one strain (bo216) persisted in some mice for 3 weeks. the greatest number of organisms (10(7)) was recovered from the ...19817287188
[campylobacter fetus subsp. jejuni as a surface contaminant of fresh and chilled pig carcasses (author's transl)].an investigation was carried out into the occurrence of campylobacter fetus subsp. jejuni on the surfaces of fresh and chilled pig carcasses. swabs were taken of the water in the scald-tank, from cut surfaces, and from cut surfaces of carcasses that had been stored at 5 degrees c for 4 days. a total of 310 swabs was investigated, of which 10 were from the scald-tank, 100 from livers, 100 from cut surfaces, and 100 from cut surfaces of carcasses stored at refrigeration temperature. a specific enr ...19817335483
acute colitis caused by campylobacter fetus ss. jejuni.campylobacter fetus ss. jejuni has been recently recognized as a common human enteric pathogen; however, the pathology and pathophysiology of the enteritis caused by this agent are still largely obscure. we report 4 patients who presented with acute abdominal pain and bloody diarrhea and who had c. fetus ss. jejuni isolated from fecal cultures. each patient showed a fourfold rise in serum igg titer to the organism isolated. in all 4 patients colonic involvement was noted on sigmoidoscopic examin ...19807351284
cellular fatty acid composition of campylobacter fetus.the cellular fatty acid composition of 29 human and animal isolates of campylobacter fetus was determined with gas-liquid chromatography. all strains contained small amounts of 3-hydroxytetradecanoic acid, suggesting the presence of lipid a. each of 13 different blood or stool isolates of c. fetus subsp. jejuni obtained from humans or fowl contained a 19-carbon cyclopropane acid which was not present in c. fetus subsp. fetus (6 strains from cattle) or c. fetus subsp. intestinalis (10 strains fro ...19807381008
campylobacter proctitis in a homosexual man. 19807436170
a survey of campylobacter bacteremia in three danish counties, 1989 to 1994.over a 5-year period (april 1989 to march 1994) bacteremia caused by campylobacter species was diagnosed in 15 patients in a population of 1.3 million in three counties in jutland province, denmark. the incidence was 0.2/100,000/year and the ratio of blood to fecal isolates, was 0.008. campylobacter coli and campylobacter jejuni accounted for 6 cases each, campylobacter fetus for 2, and in 1 case the species diagnosis was uncertain. seven patients were males and 8 females; median age was 42 (ran ...19957660078
protein shift and antigenic variation in the s-layer of campylobacter fetus subsp. venerealis during bovine infection accompanied by genomic rearrangement of sapa homologs.campylobacter fetus subsp. venerealis isolated from a case of human vaginosis was inoculated into the uterus of a c. fetus-negative heifer. isolates obtained weekly from the vaginal mucus exhibited variations in high-molecular-mass-protein profiles from that of the original inoculum, which had a dominant 110-kda s-layer protein. immunoblots of the weekly isolates with monoclonal antibody probes against the 110-kda s-layer protein and other c. fetus s-layer proteins demonstrated antigenic shifts. ...19957721688
interleukin-8 response of gastric epithelial cell lines to helicobacter pylori stimulation in vitro.gastric infection with helicobacter pylori activates a mucosal inflammatory response by mononuclear cells and neutrophils that includes expression of cytokines interleukin-1 beta (il-1 beta), il-6, tumor necrosis factor alpha, and il-8. in this study, we analyzed the il-8 response of human gastric cancer cell lines (kato iii, ags, and mkn28) to h. pylori infection in vitro. il-8 mrna expression was detected by reverse transcription-pcr amplification of rna extracted from epithelial cells after i ...19957729872
the s-layer protein from campylobacter rectus: sequence determination and function of the recombinant protein.the gene encoding the crystalline surface layer (s-layer) protein from campylobacter rectus, designated slp, was sequenced and the recombinant gene product was expressed in escherichia coli. the gene consisted of 4086 nucleotides encoding a protein with 1361 amino acids. the n-terminal amino acid sequence revealed that slp did not contain a signal sequence, but that the initial methionine residue was processed. the deduced amino acid sequence displayed some common characteristic features of s-la ...19989770285
vibrio fetus infection in man. 196014408413
broilers do not play a dominant role in the campylobacter fetus contamination of humans.campylobacter fetus causes severe infections in humans and can be isolated from various mammals and reptiles. however, although poultry are considered to be the main reservoir of campylobacter jejuni, little is known about the presence of c. fetus in poultry. thus, specific pathogen-free chickens were experimentally inoculated with a mixture of either three non-thermotolerant or four thermotolerant human strains of c. fetus. faecal samples were regularly sampled after inoculation and caeca and i ...200616914660
campylobacter fetus-associated epidural abscess and bacteremia.campylobacter fetus has been isolated as an infrequent cause of abscesses. we report a case of campylobacter fetus epidural abscess and bacteremia in a debilitated elderly man who had a fatal outcome.200919144805
campylobacter fetus translocation across caco-2 cell monolayers.campylobacter fetus is a recognized pathogen of cattle and sheep, though human infection has also been reported. ingestion of contaminated food or water is a proposed route of transmission for both humans and animals. the subsequent detection of the organism from extra-intestinal and systemic locations implies an ability to translocate across epithelial barriers. to determine how c. fetus disseminates from the intestine, caco-2 cells cultured on porous membrane supports, were used as model intes ...201020600794
new molecular microbiology approaches in the study of campylobacter fetus.campylobacter fetus infection is a substantial problem in herds of domestic cattle worldwide and a rising threat in human disease. application of comparative and functional genomics approaches will be essential to understand the molecular basis of this pathogen's interactions with various hosts. here we report recent progress in genome analyses of c. fetus ssp. fetus and c. fetus ssp. venerealis, and the development of molecular tools to determine the genetic basis of niche-specific adaptations. ...201121255368
eleutherine americana: a candidate for the control of campylobacter species.the antibacterial activity of ethanolic extracts of selected thai medicinal plants (rhodomyrtus tomentosa (aiton) hassk., quercus infectoria g. olivier, and eleutherine americana merr.) against campylobacter spp. was investigated. sixty-five campylobacter, including 39 isolates from humans and 26 isolates from chicken samples, were tested. reference campylobacter spp. that are commonly encountered in gastroenteritis were included. the ethanolic extract of e. americana demonstrated good antibacte ...201121406364
emerging dynamics of human campylobacteriosis in southern ireland.infections with campylobacter spp. pose a significant health burden worldwide. the significance of campylobacter jejuni/campylobacter coli infection is well appreciated but the contribution of non-c. jejuni/c. coli spp. to human gastroenteritis is largely unknown. in this study, we employed a two-tiered molecular study on 7194 patient faecal samples received by the microbiology department in cork university hospital during 2009. the first step, using entericbio(®) (serosep), a multiplex pcr syst ...201122077228
campylobacter fetus bacteremia in a young healthy adult transmitted by khat chewing.campylobacter fetus is a pathogen affecting almost exclusively patients with immunosuppression and chronic debilitating diseases. we report the case of a healthy young man with c. fetus bacteremia presenting with fever, hypotension and meningitis. the patient had no exposure to contaminated sources except from khat chewing, which we describe as a possible source of transmission for the first time.201122138599
raw milk and human gastrointestinal disease: problems resulting from legalized sale of "certified raw milk.". 19817028783
[human campylobacteriosis (vibriosis)]. 19807004034
effect of incubation atmosphere and temperature on isolation of campylobacter jejuni from human stools.to determine the optimal conditions for isolation of campylobacter jejuni from human fecal specimens, we compared incubation atmospheres that contained about 5, 10, and 15% oxygen with the 17% oxygen produced in candle jars and also compared incubation temperatures of 37 and 42 degrees c. at 42 degrees c, c. jejuni was isolated from all 16 specimens; however, colony sizes were larger when plates were incubated in 5 and 10% oxygen than in the other two atmospheres. at 37 degrees c some positive c ...19836850426
campylobacter-caused diarrhea. who is at risk? what to watch for.campylobacter jejuni is now recognized as one of the most common causes of human infectious diarrhea. avian species and mammals are the chief reservoirs, although the organism has been isolated from contaminated drinking water and unpasteurized milk. transmission usually occurs by the fecal-oral route through ingestion of contaminated food or water or by direct contact with feces of infected humans or animals. persons affected have abdominal pain, watery or bloody diarrhea, fever, and constituti ...19846709538
[sensitivity of campylobacter jejuni/coli to 11 antibiotics].the antimicrobial susceptibility of 106 strains of campylobacter jejuni/coli recovered from animals and humans was studied using broth microdilution panel. human and animal strains exhibit similar susceptibility, with the exception of the strains from pigs which are more resistant to erythromycin and clindamycin. among the hippurate negative strains (c. coli), these strains are also recognizable from those recovered from other hosts.19846611534
radiology in inflammatory disease of the colon: an area of increased interest for the modern clinician. 19806259078
comparison of the penner and lior methods for serotyping campylobacter spp.we compared two campylobacter serotyping systems by using 1,405 isolates of campylobacter collected from human, animal, and environmental sources during epidemiologic investigations and special studies. we found 96.1% of isolates to be typable by the penner method for heat-stable antigens, which involved the use of an indirect hemagglutination technique, and 92.1% of isolates to be typable by the lior method for heat-labile antigens, which involved the use of a slide agglutination technique and ...19854077967
campylobacter surface-layers (s-layers) and immune evasion.many pathogenic bacteria have evolved mechanisms for evading host immune systems. one evasion mechanism is manifest by the surface layer (s-layer), a paracrystalline protein structure composed of s-layer proteins (slps). the s-layer, possessed by 2 campylobacter species (c. fetus and c. rectus), is external to the bacterial outer membrane and can have multiple functions in immune avoidance. c. fetus is a pathogen of ungulates and immunocompromised humans, in whom it causes disseminated bloodstre ...200216013216
serotyping of and hippurate hydrolysis by campylobacter jejuni isolates from human patients, poultry and pigs in the netherlands.three hundred strains of campylobacter jejuni, isolated from human patients, poultry and pigs were serotyped according to the penner-lauwers system and furthermore tested for hippurate hydrolysis. serotyping showed a close relationship between human and chicken strains, whereas there was little relationship between human and pig strains. a similar conclusion was drawn from the results of the hippurate hydrolysis test: 86% of human and 94% of poultry strains were positive, whereas 91% of pig stra ...19854004203
[occurrence of campylobacter jejuni in animals and its significance for the human].the frequency of human infections caused by campylobacter (c.) jejuni is thought to be at present as significant as that of infections with salmonella. the epidemiology of human infections with c. jejuni is not well understood, although numerous species of animals are an important reservoir for this microorganism. in an overview the occurrence of c. jejuni at different domestic animals and pets is described. there is also given a report about possible direct and indirect ways for the infections ...19853895567
biochemical characteristics of vibrio sputorum and relationship to vibrio bubulus and vibrio fetus.loesche, walter j. (forsyth dental center and harvard school of dental medicine, boston, mass.), ronald j. gibbons, and sigmund s. socransky. biochemical characteristics of vibrio sputorum and relationship to vibrio bubulus and vibrio fetus. j. bacteriol. 89:1109-1116. 1965.-twenty-seven strains of vibrio sputorum were isolated from human gingival debris. all strains reduced nitrate beyond nitrite, and all produced h(2)s. none produced catalase, indole, or acetylmethylcarbinol. carbohydrates wer ...196514276104
campylobacter jejuni/coli in commercially reared beagles: prevalence and serotypes.a 5 year longitudinal study involving 187 commercially reared beagles from three suppliers was undertaken to determine prevalence and serotypes of campylobacter jejuni and c. coli. campylobacter jejuni or c. coli was isolated from the feces in 62 of 177 asymptomatic beagles and 8 of 10 dogs with diarrhea for an overall prevalence of 37%. a total of 36 isolates were serotyped on the basis of thermostable antigens with 20 antisera prepared against frequently occurring serotypes isolated from human ...19883411913
[the characteristics of thermophilic microorganisms of the genus campylobacter isolated from various sources. i. the role of the microorganisms of the genus campylobacter in human and animal pathology]. 19883387854
endotoxic activity and enterotoxigenicity of human strains of campylobacter jejuni isolated from patients in a nigerian hospital.limulus gelation assay and dermal schwartzman reaction provided a sensitive and reproducible means of testing the endotoxic activity of hospital strains of campylobacter jejuni in lagos, nigeria. all the 22 isolates of campylobacter jejuni tested for the limulus gelation assay were positive for the production of endotoxin. furthermore, the campylobacter suspensions caused a positive dermal schwartzman reaction in rabbits. the area of skin reaction was less extensive than that produced by escheri ...19892632005
efficacy of viral components of a nonabortigenic combination vaccine for prevention of respiratory and reproductive system diseases in cattle.efficacy and safety of components of an im-administered vaccine for prevention of infectious bovine rhinotracheitis virus (ibrv), parainfluenza type-3 (pi-3) virus, bovine viral diarrhea virus (bvdv), and respiratory syncytial virus (rsv) infections and campylobacteriosis and leptospirosis were evaluated in cattle, including calves and pregnant cows. challenge of immunity tests were conducted in calves for ibrv, pi-3 virus, or bvdv vaccinal components. all inoculated calves developed serum-neutr ...19892542202
serogroups of campylobacter jejuni from man and animals.a total of 186 campylobacter strains from aborted calf and sheep fetuses, from scouring dogs, rabbits and man, and from retailed poultry were isolated and examined biochemically and serologically for heat stable antigens. immune sera were produced in rabbits against penner reference strains from 1 to 60, and against two field isolates. out of 186 biochemically tested strains 179 (96.2%) proved c. jejuni and only 6 (3.2%) c. coli. one strain has been identified as c. laridis. in cattle and sheep ...19902402970
correlation between human and animal bio-serogroups of campylobacter isolates in nigeria.campylobacter enteritis is endemic in nigeria as in most other developing countries of the world. this paper examines the common biotypes and serogroups of campylobacter in human and animal isolates. the observed correlation suggests a possible animal to human route of infection in nigeria. implications on preventive and control measures are discussed.19902202840
campylobacter fetus endocarditis: two case reports and review.we report two cases of campylobacter fetus endocarditis. the first case involved a bicuspid native aortic valve in a 60-year-old woman, and the second involved a prosthetic aortic valve in a 76-year-old woman. no source of infection was identified in either case. despite antibiotic therapy, hemodynamic deterioration necessitated valve replacement; both patients recovered completely. c. fetus is an uncommon cause of human infection but may be responsible for severe illnesses such as endocarditis ...19948011832
the pet hamster as a potential reservoir of human campylobacteriosis. 19836842014
campylobacter jejuni myocarditis.a case of campylobacter jejuni (c. jejuni) myocarditis in a young man is described. c. jejuni was isolated from the patient's stools and he developed specific antibodies to this organism. tests for other etiological agents (salmonella, shigella, brucella abortus, beta-hemolytic streptococcus group a, treponema pallidum and coxsackievirus b) proved negative.19846488601
use of a selective enrichment broth for isolation of campylobacter species from human feces.isolation of campylobacter species from 1126 fecal specimens from patients with diarrhea was compared using direct plating and selective enrichment broth. the use of the enrichment broth did not increase the isolation rate, which was 4.2%. while a selective enrichment broth may have advantages where there is delay in transit to the laboratory, or where small numbers of organisms are sought, we do not recommend its use for clinical specimens from patients with diarrhea.19854094792
production and characterisation of campylobacter jejuni enterotoxin in a synthetic medium and its assay in rat ileal loops.a synthetic medium for production of campylobacter jejuni enterotoxin was developed for the purposes of its purification by modifying syncase medium, replacing sucrose with glucose, and supplementing with 0.025% sodium pyruvate, 0.25% sodium metabisulphite, 0.001% ferric chloride and 0.1% l-cysteine, adjusted to ph 6.7. culture filtrates of a human diarrhoeal and a chicken isolate, grown in this medium caused fluid accumulation ranging between 0.50-0.70 ml/cm of rat ileal loop. the kinetics of t ...19902323551
[bacteriological and serological evidence for the presence of campylobacter jejuni in poultry, animals and man (1982-1983)]. 19854070879
a case of premature labour due to campylobacter jejuni infection.a case history is described in which premature labour was thought to have been caused by chorioamnionitis secondary to infection with campylobacter jejuni. the pathogenicity of this organism in the perinatal period and in pregnancy is discussed. campylobacter species are known to cause abortion and stillbirth in cattle and sheep. the present case report supports previous evidence that campylobacter jejuni can cause infection in utero and perinatally in man, leading to abortion and preterm labour ...19826958250
unusual campylobacters in human faeces. 19863722843
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