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specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction.development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ...19921400961
occurrence, removal and seasonal variation of "thermophilic" campylobacters in a sewage treatment plant in italy.monitoring of "thermophilic" campylobacters in a sewage treatment plant in bologna (italy) has shown that incoming sewage contained a most probable number of 1630 campylobacters/100 ml. the secondary treatment in activated sludge tanks reduced 98.61% of campylobacters, 95.32% of fecal coliforms, 96.46% of fecal streptococci, 93.36% of salmonellas and 93.01% and 88.29% of bod5 and cod respectively. subsequent tertiary treatment with 3 ppm of chlorine dioxide for 15 min reduced 100% of campylobact ...19921457031
in vitro binding of campylobacter jejuni/coli outer membrane preparations to int 407 cell membranes.outer membrane fractions (oms) of nine campylobacter (c.) jejuni and two c. coli strains belonging to different serovars, from human and various animal origins, were extracted by treatment with sodium n-lauryl sarcosinate. using n-octyl-beta-d-glucopyranoside a 42-kda protein and a flagella-enriched fraction were obtained. the capacity of the crude bacterial om preparations, the purified 42-kda protein and the flagella to bind to membranes of the human embryonic intestinal cell line int 407 was ...19921549070
[an immunoenzyme test system for the detection of antibodies to campylobacter].an enzyme immunoassay system for the detection of antibodies to bacteria of the genus campylobacter in human blood serum has been developed. the system is based on the use of ethanol-treated c. jejuni and c. coli whole cells as antigen. the study of sera obtained from healthy donors in this assay has made it possible to establish the value of the tentative diagnostic titer: 320.19911801490
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in austria to erythromycin and ciprofloxacin.more than 200 strains of campylobacter (c.) jejuni/coli isolated in 1985 and 1987/88 from human fecal specimens were tested for their susceptibility to erythromycin and ciprofloxacin. their mic90 as assessed by agar dilution tests was 2.0 and 0.5 mg/l, respectively. thus, all strains were regarded as susceptible to ciprofloxacin. with 2 out of 55 strains of c. coli the mic of erythromycin was 8.0 mg/l. therefore, only 3.6% of the c. coli strains were resistant to erythromycin. all 209 strains of ...19902360966
diversity of serotypes of campylobacter jejuni and campylobacter coli isolated in laboratory animals.one hundred nineteen isolates of campylobacter jejuni and campylobacter coli from nine laboratory animal species were serotyped using antisera to 20 penner serotypes commonly isolated from cases of human enteric infections. although c. jejuni and c. coli were isolated from laboratory animals with diarrhea, the majority were cultured from asymptomatic animals (81%). seven of twenty-two isolates from animals with diarrhea were serotype 4 (32%) and three were serotype 1 (14%). sixty-one of the 119 ...19892724920
nucleotide sequence analysis of tetracycline resistance gene teto from streptococcus mutans dl5.streptococcus mutans dl5, isolated from the dental plaque of a pig, was resistant to high levels of streptomycin (sm, 20 mg/ml), erythromycin (em, 1 mg/ml), and tetracycline (tc, greater than 100 micrograms/ml), but contained no detectable plasmid dna. the smr and emr determinants were cloned from cellular dna on the self-replicating 5-kilobase-pair (kbp) ecori fragment of pam beta 1 and the 4.2-kbp cryptic plasmid pva380-1, respectively, by transformation of streptococcus sanguis challis. helpe ...19882841293
restriction endonuclease digest patterns of chromosomal dna from nitrate-negative campylobacter jejuni-like organisms.high molecular weight chromosomal dna was isolated from ten nitrate-negative campylobacters of human origin (gastric biopsies and faecal specimens). the dna was digested with various restriction endonucleases and the patterns obtained were compared with those of campylobacter coli, c. fetus subsp. fetus, c. jejuni and c. laridis reference strains. electrophoresis of hae iii digests of dna in 0.5% agarose gave excellent patterns which comprised at least 25 well-resolved bands with fragment sizes ...19852876913
small rodents and other mammals associated with mountain meadows as reservoirs of giardia spp. and campylobacter spp.sixty-five percent (469 of 722) of the fecal samples collected from small rodents in the central washington cascade mountains were positive for giardia spp. trapping studies showed that microtines of the genus microtus were heavily infected with the parasite. morphologically the cysts and trophozoites were of the giardia duodenalis type. small-rodent populations appear to maintain their infection throughout the year. our data suggest that there is no difference in the percentage of positive anim ...19873310881
serovars and biovars of campylobacter strains isolated from humans and slaughterhouse animals in northern germany.a total of 318 campylobacter strains from sporadic cases of human enteritis (109 strains) and healthy slaughterhouse animals in northern germany (209 strains) were bio- and serotyped according to the lior typing schemes. three hundred strains were typable (94.3%) and 38 serovars were identified. among human strains 28 serovars were identified with 30% of them belonging to serovar 4. strains from pigs were associated with 25 serovars, the most frequent being serovar 20 (21.2%). fourteen serovars ...19883372398
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in denmark to fourteen antimicrobial agents.the in vitro susceptibility of 95 recent danish human clinical isolates of campylobacter jejuni and campylobacter coli to 14 antimicrobial agents was determined by an agar dilution technique. c. jejuni and c. coli were considered together. doxycycline, ciprofloxacin, gentamicin, clindamycin, nitrofurantoin and erythromycin were the most active drugs. 2% of the strains tested were resistant to erythromycin (mic greater than 3.1 micrograms/ml). therefore susceptibility testing is suggested if anti ...19873630712
naturally occurring auxotrophs of campylobacter jejuni and campylobacter coli.the nutritional requirements for 439 campylobacter jejuni isolates and 46 campylobacter coli isolates were determined by using a previously described chemically defined medium, campylobacter defined medium. with this medium, 45% of both human and nonhuman c. jejuni isolates demonstrated auxotrophic requirements. none of the 46 c. coli isolates studied demonstrated requirements for amino acids on campylobacter defined medium. the most common auxotrophic requirement among c. jejuni isolates was fo ...19873654939
an acid extract as a common antigen in campylobacter coli and campylobacter jejuni strains.an acid extract prepared from a single strain of campylobacter jejuni reacted with rabbit antisera against 21 strains of c. jejuni and campylobacter coli. the immunogen is resistant to formalin and to exposure to 100 degrees c for 30 min. the antigenic extract may have practical applications in human serology.19836343426
pathogenicity of campylobacter jejuni and campylobacter coli strains in the pregnant guinea pig model.pathogenicity of 17 campylobacter isolates for pregnant guinea pigs was investigated. of 14 isolates, 12 (86%) produced rates of abortion ranging from 13% to 87%. two isolates did not produce abortion. reference strains of c fetus subsp venerealis produced abortion in 60% to 87% and c fetus subsp fetus produced abortion in 60% of the guinea pigs. inoculated organisms were recovered from uterus, blood, liver, kidney, spleen, and gallbladder of the guinea pigs at rates as high as 83% for 2 ovine i ...19836650962
diffusion of thermophilic campylobacter in the pesaro-urbino area (italy) from 1985 to 1992.the results of research on the spreading of campylobacter in the pesaro-urbino area carried out from 1985 to 1992 are presented. materials of different origin were examined: 822 samples of human faeces, 533 animal rectal swabs, 192 samples of domestic sewage, 48 of river water, 96 of sea water and 632 of various types of food. two hundred and nine strains of campylobacter were isolated (9%), most of which were campylobacter jejuni (80%), with particular frequency in food products (chicken carcas ...19957489778
differentiation of campylobacter jejuni and campylobacter coli strains by using restriction endonuclease dna profiles and dna fragment polymorphisms.the chromosomal dna fragment patterns from a total of 169 campylobacter jejuni and campylobacter coli isolates from poultry and humans were analyzed by using dna restriction endonucleases clai and ecorv. the dna restriction patterns produced by clai and ecorv consisted of unique dna fragments of 9 to 9.5 kb and 3.5 kb generated with clai and a single unique fragment of 3.0 kb produced by ecorv. these patterns were obtained with all strains of c. jejuni tested. the dna restriction patterns were f ...19957615717
a survey of campylobacter bacteremia in three danish counties, 1989 to 1994.over a 5-year period (april 1989 to march 1994) bacteremia caused by campylobacter species was diagnosed in 15 patients in a population of 1.3 million in three counties in jutland province, denmark. the incidence was 0.2/100,000/year and the ratio of blood to fecal isolates, was 0.008. campylobacter coli and campylobacter jejuni accounted for 6 cases each, campylobacter fetus for 2, and in 1 case the species diagnosis was uncertain. seven patients were males and 8 females; median age was 42 (ran ...19957660078
prevalence of campylobacter in pigs during fattening; an epidemiological study.numerous epidemiological reports implicate foods of animal origin as vehicles of human campylobacteriosis. pigs are probably an important reservoir of campylobacter and a potential source of human infection. in order to improve our knowledge of the epidemiology of campylobacter in pigs, the prevalence of campylobacter and its contamination of feed were monitored in eight pig farms. faeces samples of pigs aged 11 and 22 weeks, and samples of rectal, ileal and gastric content at a slaughterhouse w ...19937907195
bacteraemia caused by campylobacter spp.the genus campylobacter has become increasingly recognised as the cause of various infections. campylobacter jejuni and c coli cause acute gastroenteritis in man all over the world. c jejuni enteritis can lead to bacteraemia, but its actual incidence remains unknown. seven cases of bacteraemia caused by c jejuni or c coli are reported, from the blood of seven patients: five immune deficient adults; a newborn baby; and a patient who had had abdominal surgery. patients who develop diarrhoea as a r ...19948132835
rgfgigs is an amino acid sequence required for acetyl coenzyme a binding and activity of human spermidine/spermine n1acetyltransferase.polyamine catabolism is rate limited by spermidine/spermine n1-acetyltransferase (ssat). although the amino acid sequence of ssat is known, the substrate binding and catalytic sites are not. the goal of this study was to define the region responsible for acetyl coenzyme a binding. human ssat contains a region of 20 amino acids homologous to several microbial antibiotic n-acetyltransferases. the highest homology is represented in the campylobacter coli streptothricin acetyltransferase sat4 gene, ...19968702554
in vitro studies of campylobacter jejuni/coli strains from hens and humans regarding adherence, invasiveness, and toxigenicity.campylobacter jejuni/coli strains from hens and humans were compared for their ability to adhere to and invade hep-2-cells and for toxigenicity to cho-cells. in both hen and human strains, invasiveness was higher among non-toxigenic strains than among toxigenic ones. the frequency of adherence, invasiveness, and toxigenicity was the same in hen and human strains.19958719204
development of a rapid and specific colony-lift immunoassay for detection and enumeration of campylobacter jejuni, c. coli, and c. lari.contamination of retail poultry by campylobacter spp. is a significant source of human diarrheal disease. we have developed a colony-lift immunoassay (cli) for the detection of campylobacter jejuni, c. coli, and c. lari isolated from such sources and grown on selective agar medium or on filter membranes. this technique has been successfully utilized to quantify campylobacter colonies within 18 to 28 h after sampling. hydrophobic, high-protein-binding membranes were prewet with methanol and used ...19968914757
comparison of a novel microaerobic system with three other gas-generating systems for the recovery of campylobacter species from human faecal samples.three commercial gas-generating systems--campygen (oxoid, uk), oxoid br56 (oxoid, uk), and campypak plus (becton dickinson, usa)--and the evacuation replacement technique were compared for the recovery of campylobacter spp. from 500 human faecal samples collected from patients with gastroenteritis. four hundred fifty faecal samples were tested upon receipt in the laboratory. fifty faecal samples that had been previously found to be positive for campylobacter spp. were tested retrospectively; the ...19979447907
antimicrobial susceptibilities of campylobacter jejuni and coli by using e-test in taiwan.to report the in vitro antibiotic susceptibility of campylobacter species, we determined the mics of 6 antibiotics by e-test for 93 human clinical strains and 35 chicken strains. the 6 antimicrobial agents tested were gentamicin, erythromycin, clindamycin, tetracycline, ciprofloxacin, and nalidixic acid. isolates from humans were significantly more susceptible than chicken strains to erythromycin, clindamycin and ciprofloxacin. nearly all of the human and chicken strains were susceptible to gent ...19989670357
detection of cytolethal distending toxin activity and cdt genes in campylobacter spp. isolated from chicken carcasses.this study was designed to determine whether isolates from chicken carcasses, the primary source of campylobacter jejuni and campylobacter coli in human infections, commonly carry the cdt genes and also whether active cytolethal distending toxin (cdt) is produced by these isolates. campylobacter spp. were isolated from all 91 fresh chicken carcasses purchased from local supermarkets. campylobacter spp. were identified on the basis of both biochemical and pcr tests. of the 105 isolates, 70 (67%) ...199910103243
the absence of cecal colonization of chicks by a mutant of campylobacter jejuni not expressing bacterial fibronectin-binding protein.campylobacter jejuni is a common cause of human gastrointestinal illness throughout the world. infections with c. jejuni and campylobacter coli are frequently acquired by eating undercooked chicken. the ability of c. jejuni to become established in the gastrointestinal tract of chickens is believed to involve binding of the bacterium to the gastrointestinal surface. a 37-kd outer membrane protein, termed cadf, has been described that facilitates the binding of campylobacter to fibronectin. this ...199910494431
antimicrobial susceptibilities of campylobacter strains isolated from food animals in belgium.campylobacter spp. are a frequent cause of diarrhoea in man, originating mostly from poultry. it has been suggested that the veterinary use of antibiotics is largely responsible for resistance in human isolates, particularly to quinolones. during a 6 month period from june to december 1998, 677 campylobacter isolates were obtained from healthy poultry and pigs. samples were taken at belgian slaughterhouses. species identification was performed by biochemical tests, multiplex pcr and sds-page of ...200111481294
bacterial genetic fingerprint: a reliable factor in the study of the epidemiology of human campylobacter enteritis?the rate of human intestinal infections with more than a single campylobacter strain was determined and the genetic variabilities of campylobacter strains throughout an infection episode were investigated by means of pulsed-field gel electrophoresis (pfge) and enterobacterial repetitive intergenic consensus sequence pcr (eric-pcr). for 48 and 49 of 50 patients, all isolates from one sample showed identical patterns by pfge and eric-pcr, respectively. throughout an infection episode in 47 of 52 p ...200111682547
evaluation of a pcr/dna probe colorimetric membrane assay for identification of campylobacter spp. in human stool specimens.dna was extracted from 50 human stool specimens using the qiaamp dna stool minikit. pcr amplification was followed by post-pcr hybridization to dna probes specific for the campylobacter genus, campylobacter jejuni, and campylobacter coli in a colorimetric membrane assay. thirty-two of 38 culture-positive specimens were pcr/dna probe positive for c. jejuni. the assay is rapid and simple and can be applied to stool specimens for the detection of campylobacter.200111682549
use of a multiplex pcr-based assay to differentiate campylobacter jejuni and campylobacter coli strains isolated from human and animal sources. 200211749144
development of a combined filtration-enrichment culture followed by a one-step duplex pcr technique for the rapid detection of campylobacter jejuni and c. coli in human faecal samples.a new combined filtration-enrichment culture followed by a pcr technique for the rapid detection of campylobacterjejuni and c. coli in human faeces has been developed. only bacteria that passed through the membrane could multiply in the enrichment culture; target bacteria were detected by a one-step duplex pcr technique with combinations of primers that are specific for different campylobacter spp., which should allow for the detection of a mixed infection in a single patient. a falcon cell-cult ...200211800478
antimicrobial resistance and plasmid profiles of campylobacter jejuni and campylobacter coli from human and animal sources.the purpose of this study was to determine the susceptibility of campylobacter jejuni and campylobacter coli isolates to antimicrobial agents and to investigate the presence of plasmid dna.200211849513
human campylobacteriosis in developing countries.campylobacteriosis is a collective description for infectious diseases caused by members of the bacterial genus campylobacter. the only form of campylobacteriosis of major public health importance is campylobacter enteritis due to c. jejuni and c. coli. research and control efforts on the disease have been conducted more often in developed countries than developing countries. however, because of the increasing incidence, expanding spectrum of infections, potential of hiv-related deaths due to ca ...200211927019
speciating campylobacter jejuni and campylobacter coli isolates from poultry and humans using six pcr-based assays.six previously published polymerase chain reaction (pcr) assays each targeting different genes were used to speciate 116 isolates previously identified as campylobacter jejuni using routine microbiological techniques. of the 116 isolates, 84 were of poultry origin and 32 of human origin. the six pcr assays confirmed the species identities of 31 of 32 (97%) human isolates and 56 of 84 (67%) poultry isolates as c. jejuni. twenty eight of 84 (33%) poultry isolates were identified as campylobacter c ...200212435503
antimicrobial resistance of campylobacter jejuni and campylobacter coli isolates from broiler chickens isolated at an irish poultry processing plant.the antibiotic susceptibility of campylobacter jejuni and campylobacter coli isolates from broiler chickens were determined in order to evaluate the level of antibiotic resistance of campylobacter species in the irish poultry industry.200312680938
detection of conserved n-linked glycans and phase-variable lipooligosaccharides and capsules from campylobacter cells by mass spectrometry and high resolution magic angle spinning nmr spectroscopy.glycomics, the study of microbial polysaccharides and genes responsible for their formation, requires the continuous development of rapid and sensitive methods for the identification of glycan structures. in this study, methods for the direct analysis of sugars from 108 to 1010 cells are outlined using the human gastrointestinal pathogen, campylobacter jejuni. using capillary-electrophoresis coupled with sensitive electrospray mass spectrometry, we demonstrate variability in the lipid a componen ...200312716884
prevalence of multiple antibiotic resistance in 443 campylobacter spp. isolated from humans and animals.in view of recent findings that a multidrug efflux pump cmeabc exists in campylobacter jejuni, 391 c. jejuni and 52 campylobacter coli of human and animal origin were examined for a multidrug resistance phenotype.200312917241
comparison of human and porcine strains of campylobacter coli. 200312930063
in vitro activities of new fluoroquinolones against campylobacter jejuni and campylobacter coli isolates obtained from humans in 1980 to 1982 and 1997 to 2001.the antibacterial activities of three newly developed fluoroquinolones (gatifloxacin, levofloxacin, and moxifloxacin) against a total of 307 gastrointestinal human isolates of campylobacter jejuni and campylobacter coli collected during 1980 to 1982 and 1997 to 2001 were examined and compared to those of ciprofloxacin and the unrelated antibacterial agents, clarithromycin, erythromycin, and tetracycline by using the agar plate dilution method. all of the fluoroquinolones exhibited a good activit ...200312936999
characterization of erythromycin resistance in campylobacter coli and campylobacter jejuni isolated from pig offal in new zealand.to determine the level and mechanism(s) of antimicrobial resistance in campylobacter isolates obtained from human and environmental sources from south canterbury, new zealand.200415186451
a real-time multiplexed pcr assay for rapid detection and differentiation of campylobacter jejuni and campylobacter coli.campylobacter species are the leading agents of bacterial gastroenteritis worldwide. c. jejuni and c. coli together are responsible for more than 95% of all cases of campylobacter-induced diarrheal disease in the united states. detection of campylobacteria in clinical samples by conventional culture is problematic and slow due to their complex taxonomy, fastidious growth requirements, and biochemical inertness. the current study describes a rapid, sensitive, and specific real-time polymerase cha ...200415271389
characterization of lactobacillus coryniformis dsm 20001t surface protein cpf mediating coaggregation with and aggregation among pathogens.phenotypic characterization of aggregation phenotypes of lactobacillus coryniformis revealed that strain dsm 20001t coaggregated with escherichia coli k88, campylobacter coli, and campylobacter jejuni but not with other human pathogens. in addition, cells of these pathogens aggregated in the presence of the spent culture supernatant (scs) of strain dsm 20001t. cells of e. coli k88 remained viable in the coaggregates and aggregates for up to 24 h. both coaggregation and aggregation (co/aggregatio ...200415574903
molecular characterization of campylobacter isolated from chickens and humans in northern thailand.a study was conducted in northern thailand to characterize campylobacter spp isolated from chickens at farms, slaughterhouse, and chicken meat at fresh markets, and stools from chicken farmers as well as neighboring crop farmers who served as controls. the campylobacter isolates were collected during the rainy months of the years 2000, 2001, and 2002. standard methods were used for primary isolation and identification of camplobacter and the resulting isolates were frozen and stored in 30% glyce ...200415689085
[antimicrobial susceptibility of campylobacter jejuni and campylobacter coli isolated from human diarrheic samples].one hundred forty-seven campylobacter were isolated using 3,204 samples taken from sporadic diarrheic patients from january 2001 to december 2003. the detection rate of campylobacter in 16 to 30 year old patients (12.9%, 83/641) was significantly higher than that in patients less than 16 years of age, 5%, (29/1,155) (p < 0.001) and more than 30 years of age, 2.5% (35/1,408) (p < 0.001), respectively. the highest detection rate was obtained from the stool of males in the 16 to 30 year old range d ...200515977557
occurrence of campylobacter species in poultry forms in lagos area of nigeria.samples of poultry droppings were collected from fifty poultry farms in lagos area of nigeria. campylobacter species were isolated from serially diluted samples using bolton selective enrichment medium under microaerophilic condition at 42 degrees c. samples of water, both treated and untreated used in the farms were also analysed in the same manner. fifteen campylobacter strains were isolated from the droppings. all the strains were identified using biochemical characteristics differentiating c ...200516334275
genotyping of campylobacter coli and c. jejuni from retail chicken meat and humans with campylobacteriosis in slovenia and bosnia and herzegovina.thermotolerant campylobacter jejuni and c. coli are one of the major causes of bacterial foodborne enteric infection. consuming and/or handling poultry meat is the most consistent risk factor, linked to the high prevalence of campylobacters in retail poultry meat. the aim of the present study was to ascertain the genetic diversity and/or possible specificity of thermotolerant campylobacter isolates according to species (c. coli, c. jejuni), isolation source (retail chicken meat and human clinica ...200616712997
study on the prevalence of campylobacter spp. from chicken meat in hanoi, vietnam.campylobacter spp. is considered to be the most common bacterial cause of human gastroenteritis worldwide. in developing countries, campylobacter spp. diarrhea is an important cause of childhood morbidity. chicken meat is known to be a major source of campylobacteriosis infection in the world. the purpose of this study was to investigate the prevalence of campylobacter spp. in chicken meat. a total of 100 samples from breast part of chicken carcass were collected from retail market in hanoi. the ...200617135525
prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from raw chicken meat and human stools in korea.prevalence of campylobacter in raw chicken meat and human stools and subsequent antibiotic resistance profiles of the pathogenic isolates obtained from 2000 through 2002 were investigated. campylobacter jejuni and campylobacter coli were isolated from 570 of the 923 raw chicken meat samples collected from traditional markets, large retail stores, or department stores in korea, resulting in the isolation rate of 61.8%. a total of 579 campylobacter isolates were obtained from raw chicken (36.3% fo ...200617186659
evaluation of genetic markers and molecular typing methods for prediction of sources of campylobacter jejuni and c. coli infections.campylobacter jejuni and c. coli isolates from poultry, cattle, and humans were studied using pulsed-field gel electrophoresis (pfge) and pcr of candidate livestock-associated marker genes. human isolates showed 5.7 and 61% overlap with cattle and poultry isolates, respectively, by use of pfge. no unambiguous association was found between marker genes (the cj1321 and cj1324 genes) and livestock-associated isolates.200717220252
identification of genomic differences between campylobacter jejuni subsp. jejuni and c. jejuni subsp. doylei at the nap locus leads to the development of a c. jejuni subspeciation multiplex pcr method.the human bacterial pathogen campylobacter jejuni contains two subspecies: c. jejuni subsp. jejuni (cjj) and c. jejuni subsp. doylei (cjd). although cjd strains are isolated infrequently in many parts of the world, they are obtained primarily from human clinical samples and result in an unusual clinical symptomatology in that, in addition to gastroenteritis, they are associated often with bacteremia. in this study, we describe a novel multiplex pcr method, based on the nitrate reductase (nap) lo ...200717328805
relapsing campylobacter coli bacteremia with reactive arthritis in a patient with x-linked agammaglobulinemia.a patient genetically diagnosed with x-linked agammaglobulinemia repeatedly developed bacteremia due to campylobacter coli (c. coli) for one year and seven months in spite of immunoglobulin replacement therapy. throughout the clinical course, c. coli with identical genetic patterns was repeatedly isolated from both blood and stool cultures, thus indicating that the patient had latent intestinal infection. the bacteremia was always accompanied by reactive arthritis. since the immunoglobulin level ...200717473499
prevalence and antimicrobial resistance of campylobacter in us dairy cattle.to obtain an overview of the prevalence and antimicrobial resistance of campylobacter in faeces of us dairy cows in 2002.200717578422
antibiotic resistance and resistance mechanisms in campylobacter jejuni and campylobacter coli.campylobacter jejuni and campylobacter coli are recognized as the most common causative agents of bacterial gastroenteritis in the world and infections with these organisms occur more frequently than do infections due to salmonella species, shigella species, or escherichia coli 0157:h7. the incidence of human campylobacter infections has increased markedly in both developed and developing countries worldwide and, more significantly, so has the rapid emergence of antibiotic-resistant campylobacte ...200718031331
development of a novel triplex pcr assay for the detection and differentiation of thermophilic species of campylobacter using 16s-23s rdna internal transcribed spacer (its) region.campylobacter species are significantly implicated in human gastrointestinal infections. of 20 species of campylobacter, c. jejuni, c. coli and c. lari have been considered as the most important causative agents of human infections. in order to better understand the occurrence and epidemiology of these thermophilic campylobacter species, an improved and rapid detection method is warranted. a novel triplex polymerase chain reaction (pcr) assay was developed based on the variable 16s-23s rdna inte ...200718045440
role of poultry meat in sporadic campylobacter infections in bosnia and herzegovina: laboratory-based study.to investigate genetic diversity and specificity of campylobacter jejuni and campylobacter coli strains isolated from humans, retail poultry meat, and live farm chickens in zenica-doboj canton, bosnia and herzegovina, and identify the role of poultry meat in sporadic campylobacter infections.200718074419
development and evaluation of immunochromatographic assay for simple and rapid detection of campylobacter jejuni and campylobacter coli in human stool specimens.an immunochromatographic assay (campy-ica) using a newly generated single monoclonal antibody against a 15-kda cell surface protein of campylobacter jejuni was developed. when cell suspensions of 86 c. jejuni strains and 27 campylobacter coli strains were treated with a commercially available bacterial protein extraction reagent and the resulting extracts were tested with the campy-ica, they all yielded positive results. the minimum detectable limits for the c. jejuni strains ranged from 1.8 x 1 ...200818256225
comparative performance of different pcr assays for the identification of campylobacter jejuni and campylobacter coli.the sensitivity and specificity of 7 pcr assays described for the identification of campylobacter jejuni and campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of c. jejuni, c. coli, campylobacter lari and related campylobacter, helicobacter and arcobacter species. based on a preliminary evaluation, one multiplex test was excluded from further evaluation. the various assays differed considerably in sensitivity and specificit ...200818272350
genetic diversity of campylobacter on broiler carcasses collected preevisceration and postchill in 17 u.s. poultry processing plants.campylobacter jejuni and campylobacter coli are the most important human enteropathogens among the campylobacters. the objective of this study was to determine how diversity in campylobacter populations found on chicken carcasses collected from 17 broiler processing plants in the united states is impacted by processing. genetic diversity was determined for up to four isolates per carcass by sequencing the short variable region (svr) of the flaa locus. on 70% of campylobacter-positive carcasses, ...200919205463
campylobacter genotyping to determine the source of human infection.campylobacter species cause a high proportion of bacterial gastroenteritis cases and are a significant burden on health care systems and economies worldwide; however, the relative contributions of the various possible sources of infection in humans are unclear.200919275496
prevalence of thermotolerant campylobacter in partridges (perdix perdix).to estimate the prevalence of thermotolerant campylobacter spp. in commercially reared partridges (perdix perdix) in southern italy.200919552770
spatiotemporal homogeneity of campylobacter subtypes from cattle and sheep across northeastern and southwestern scotland.source attribution using molecular subtypes has implicated cattle and sheep as sources of human campylobacter infection. whether the campylobacter subtypes associated with cattle and sheep vary spatiotemporally remains poorly known, especially at national levels. here we describe spatiotemporal patterns of prevalence, bacterial enumeration, and subtype composition in campylobacter isolates from cattle and sheep feces from northeastern (63 farms, 414 samples) and southwestern (71 farms, 449 sampl ...200919700557
prevalence of campylobacter spp. in raw retail poultry on sale in northern ireland.a year-long survey of fresh, retail poultry products on sale in northern ireland was undertaken to define the prevalence of campylobacter spp. by using protocols based on iso (standard) 10272-1:2006. incubation at 37 and 42 degrees c was undertaken to increase the diversity of isolates obtained. overall, 652 isolates were identified as campylobacter spp. by using pcr and amplified fragment length polymorphic typing. phenotyping wrongly identified 21% of isolates. prevalences of campylobacter fou ...200919777882
[molecular investigation of relatedness of campylobacter coli isolated from child with campylobacteriosis and healthy, household dog].thermotolerant species of campylobacter (mainly c. jejuni and c. coli) are among the most frequently isolated bacterial agents of gastroenteritis in many developed countries. c. coli is less prevalent than c. jejuni. the main reservoir of c. coli is swine, however this pathogen may also be found in poultry, cattle and pets. genotypic methods have successfully been applied to the subtyping of campylobacter species in epidemiological investigation. in the present study we report a case of isolatio ...200920120926
generation of the pathogenic r5-tropic simian/human immunodeficiency virus shivad8 by serial passaging in rhesus macaques.a new pathogenic r5-tropic simian/human immunodeficiency virus (shiv) was generated following serial passaging in rhesus macaques. all 13 animals inoculated with shiv(ad8) passaged lineages experienced marked depletions of cd4(+) t cells. ten of these infected monkeys became normal progressors (nps) and had gradual losses of both memory and naïve cd4(+) t lymphocytes, generated antiviral cd4(+) and cd8(+) t cell responses, and sustained chronic immune activation while maintaining variable levels ...201020147396
prevalence and antimicrobial resistance of campylobacter species isolated from raw camel, beef, lamb, and goat meat in iran.campylobacter spp. are one of the most common causes of acute bacterial gastroenteritis in human beings which are transmitted mostly via food originating from animals. this study was conducted to determine the prevalence and antimicrobial resistance of campylobacter spp. isolated from retail raw meats in iran. from june 2008 to june 2009, a total of 722 raw meat samples from camel (n = 107), beef (n = 190), lamb (n = 225), and goat (n = 180) were purchased from randomly selected retail outlets i ...201020184450
prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from cattle between 2002 and 2006 in france.feces from 2,255 cattle (calves, young beef cattle, and culled cows) were collected at slaughter from nine departments across france. campylobacter was recovered from 16.5% of the 2,255 samples (c. jejuni from 12.8% and c. coli from 3.7%), predominantly from calves. antimicrobial resistance to six antibiotics of medical and/or veterinary interest was tested with the e-test. resistance to tetracycline was found in most isolates (52.8% of c. jejuni isolates and 88.1% of c. coli isolates) in contra ...201020501032
direction of neutrophil movements by campylobacter-infected intestinal epithelium.campylobacter jejuni and campylobacter coli together represent the leading bacterial cause of human enteritis. however, the pathogenesis of this disease is poorly understood. infection results in the formation of crypt abscesses resulting from the migration of neutrophils across the intestinal epithelium and into the intestinal crypts. in this study, we model this process in vitro and show that campylobacter infection of epithelium results in a quantifiable increase in the directed movement of n ...201020934530
evolution of an agriculture-associated disease causing campylobacter coli clade: evidence from national surveillance data in scotland.the common zoonotic pathogen campylobacter coli is an important cause of bacterial gastroenteritis worldwide but its evolution is incompletely understood. using multilocus sequence type (mlst) data of 7 housekeeping genes from a national survey of campylobacter in scotland (2005/6), and a combined population genetic-phylogenetics approach, we investigated the evolutionary history of c. coli. genealogical reconstruction of isolates from clinical infection, farm animals and the environment, reveal ...201021179537
new methods for detection of campylobacters in stool samples in comparison to culture.campylobacter species, especially campylobacter jejuni and campylobacter coli, are a major cause of human bacterial enteritis. current detection in stools is done essentially by culture on selective and nonselective media with filtration. these methods were compared to 2 molecular biology methods, an in-house real-time pcr and a multiplex pcr named seeplex diarrhea ace detection, and 3 immunoenzymatic methods, premier campy, ridascreen campylobacter, and immunocard stat!campy. out of 242 stool s ...201121209172
pulsed field gel electrophoresis typing of human and retail foodstuff campylobacters: an irish perspective.campylobacter enteritis is a zoonosis, an infectious disease transmissible under normal conditions from vertebrate animals to man, presenting a major global public health burden. in this study, pulsed field gel electrophoresis (pfge) was employed to identify common genotypes in a collection of 600 campylobacter isolates in order to investigate if profiles obtained from retail samples of foodstuffs matched genotypes causing illness in the community in ireland. the campylobacters were isolated fro ...201021356447
eleutherine americana: a candidate for the control of campylobacter species.the antibacterial activity of ethanolic extracts of selected thai medicinal plants (rhodomyrtus tomentosa (aiton) hassk., quercus infectoria g. olivier, and eleutherine americana merr.) against campylobacter spp. was investigated. sixty-five campylobacter, including 39 isolates from humans and 26 isolates from chicken samples, were tested. reference campylobacter spp. that are commonly encountered in gastroenteritis were included. the ethanolic extract of e. americana demonstrated good antibacte ...201121406364
rapid identification and quantification of campylobacter coli and campylobacter jejuni by real-time pcr in pure cultures and in complex samples.abstract: background: campylobacter spp., especially campylobacter jejuni (c. jejuni) and campylobacter coli (c. coli), are recognized as the leading human foodborne pathogens in developed countries. livestock animals carrying campylobacter pose an important risk for human contamination. pigs are known to be frequently colonized with campylobacter, especially c. coli, and to excrete high numbers of this pathogen in their faeces. molecular tools, notably real-time pcr, provide an effective, rapid ...201121600037
the prevalence and genetic diversity of campylobacter spp. in domestic 'backyard' poultry in canterbury, new zealand.campylobacteriosis is the most commonly notified illness in new zealand. whilst the importance of commercial poultry in campylobacteriosis is well established, little is known about the possible role of chickens kept at home as a direct animal/faecal contact or consumption exposure pathway. the aim of this study was to determine the prevalence and genetic diversity of campylobacter spp. in domestic backyard chicken flocks in the canterbury region of new zealand. poultry faecal samples were colle ...201121824372
emerging dynamics of human campylobacteriosis in southern ireland.infections with campylobacter spp. pose a significant health burden worldwide. the significance of campylobacter jejuni/campylobacter coli infection is well appreciated but the contribution of non-c. jejuni/c. coli spp. to human gastroenteritis is largely unknown. in this study, we employed a two-tiered molecular study on 7194 patient faecal samples received by the microbiology department in cork university hospital during 2009. the first step, using entericbio(®) (serosep), a multiplex pcr syst ...201122077228
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