molecular characterization of a chemotaxis operon in the oral spirochete, treponema denticola.a chemotaxis gene cluster from treponema denticola (td), a pathogenic spirochete associated with human periodontal diseases, was cloned, sequenced, and analyzed. the gene cluster contained three chemotaxis (che) genes (chea, chew, and chey) and an open reading frame (chex) that is homologous with treponema pallidum (tp) and borrelia burgdorferi (bb) chex. the td che genes have the same transcriptional orientation with a sigma 70-like promoter located upstream of chea and a stem-loop structure ch ...199910333522
genetic characterization of an oral treponeme isolated from human subgingival plaque.we examined the genetic characteristics of a human oral treponeme isolated from subgingival plaque of a patient with periodontal disease that could not be assigned to any of the previously described oral treponema species. the guanine-plus-cytosine content of its dna was 51 mole%. the levels of dna-dna relatedness of the organism to other treponema species, including treponema denticola, treponema vincentii, treponema socranskii, treponema pallidum and treponema phagedenis, were less than 30%. t ...19979467407
release and activation of human neutrophil matrix metallo- and serine proteinases during phagocytosis of fusobacterium nucleatum, porphyromonas gingivalis and treponema denticola.the phagocytic ingestion of reference strains and clinical isolates of fusobacterium nucleatum, porphyromonas gingivalis, and treponema denticola by polymorphonuclear leukocytes (pmns) and the concomitant release of pmn granule proteinases were studied by specific functional and immunological assays. pmns were incubated with the microorganisms anaerobically at 37 degrees c for indicated time periods. the suspensions and pellets were used for phagocytic ingestion assay and electron microscopic st ...19979144046
herpesviruses in human periodontal disease.recent studies have identified various herpesviruses in human periodontal disease. epstein-barr virus type 1 (ebv-1) infects periodontal b-lymphocytes and human cytomegalovirus (hcmv) infects periodontal monocytes/ macrophages and t-lymphocytes. ebv-1, hcmv and other herpesviruses are present more frequently in periodontitis lesions and acute necrotizing ulcerative gingivitis-lesions than in gingivitis or periodontally healthy sites. reactivation of hcmv in periodontitis lesions tends to be asso ...200010791704
treponema maltophilum sp. nov., a small oral spirochete isolated from human periodontal lesions.a novel culture medium for cultivation of fastidious oral anaerobes is described. this medium, omiz-pat, consists of a rich chemically defined basal medium supplemented with asialofetuin, as well as yeast extract and neopeptone fractions. addition of 1 mg of rifampin per liter and 100 mg of fosfomycin per liter allowed routine isolation of spirochetes by a limit dilution method in 96-well plates containing liquid omiz-pat. in addition to members of the four previously recognized species of oral ...19968782684
trypsin-like activity levels of treponema denticola and porphyromonas gingivalis in adults with periodontitis.treponema denticola (td) and porphyromonas gingivalis (pg) are associated with human moderate and severe adult periodontal diseases. this study quantifies these two anaerobes and their trypsin-like (tl) activities in subgingival plaque collected from both clinically healthy and periodontally diseased sites of human periodontitis patients. antigen levels of the microorganisms were determined by monoclonal antibodies and tl activities were measured by the fluorescent substrate z-gly-gly-arg-afc in ...19947989615
purification and general properties of an oligopeptidase from treponema denticola atcc 35405--a human oral endo-acting oligopeptidase (opase) was purified to homogeneity from the cells of treponema denticola atcc 35405--a human oral spirochete--by a procedure that comprised a mild triton x-100 extraction (which disintegrates the outer membrane but leaves the cells morphologically intact) and four successive fast protein liquid chromatographic steps of the extract. the activity of this oligopeptidase (formerly named "trypsin-like" enzyme and "bana-peptidase") together with the proteinase activities ...19957864624
cellular source, activation and inhibition of dental plaque plaque is the major aetiological factor in periodontal diseases and contains several proteolytic enzymes. the origin of these proteinases is, however, poorly studied. this study was undertaken to characterize collagenase present in dental plaque of adult periodontitis patients. vertebrate-type rather than bacterial-derived collagenase activity was detected in extracts of both supra- and subgingival dental plaque extracts of adult periodontitis patients. dental plaque collagenase was found ...19957593702
suppression of human lymphocyte responses by oral spirochetes: a monocyte-dependent phenomenon.spirochetes have been implicated in the pathogenesis of several human infections including syphilis, yaws, lyme disease, and periodontal diseases. we examined soluble sonic extracts of oral spirochetes (treponema denticola and t. vincentii) for their ability to alter human lymphocyte function. these organisms were isolated from subgingival plaque of patients with periodontitis. we found that sonicates of several but not all strains of t. denticola caused a dose-dependent inhibition of human lymp ...19846699406
morphological and biochemical differentiation of three types of small oral spirochetes.thirty strains of human oral anaerobic spirochetes were isolated in three different media: veal heart infusion-ascitic fluid, spirolate-brain heart infusion-rabbit serum, and supplemented pplo broth. the morphological and biochemical characteristics of the isolates permitted their differentiation into three distinct species: treponema denticola, t. macrodentium, and t. oralis (proposed new species). these species could be differentiated as follows. organisms of the t. denticola type had a "2-4-2 ...19695788714
herpesviral-bacterial interactions in aggressive periodontitis.human cytomegalovirus, epstein-barr virus and herpesvirus co-infections occur with significantly higher frequency in actively progressing than in stable periodontitis sites of adolescents and young adults. also, periodontal presence of cytomegalovirus and epstein-barr virus is associated with increased occurrence of subgingival porphyromonas gingivalis, bacteroides forsythus, dialister pneumosintes, prevotella intermedia, prevotella nigrescens, treponema denticola and actinobacillus actinomycete ...200312716334
quantitative relationship of treponema denticola to severity of periodontal disease.the treponema denticola content of plaque was quantitatively estimated for samples taken from periodontitis patients as well as periodontally healthy subjects among two separate human populations. the populations studied included military volunteers and civilians at a university dental clinic. the plaque samples from each population were grouped according to pocket depth measurements at the collection site. a biotin-avidin enzyme-linked immunosorbent assay procedure was developed with a monoclon ...19883346072
characterization of monoclonal antibodies to two treponema denticola serotypes by the indirect fluorescent-antibody assay.members of the genus treponema have been implicated as possible aetiologic agents of periodontal disease. previously developed murine monoclonal antibodies (mabs) to two treponema denticola strains were characterized by an indirect fluorescent-antibody (ifa) technique. fifteen t. denticola strains and 32 other bacteria commonly isolated from periodontal pockets were used in the screening process. two monoclonal antibodies to t. denticola atcc 33521, serotype b, reacted with five t. denticola iso ...19912030646
identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases.activation of latent human fibroblast-type and neutrophil interstitial procollagenases as well as degradation of native type i collagen by supra- and subgingival dental plaque extracts, an 80-kda trypsinlike protease from porphyromas gingivalis (atcc 33277), a 95-kda chymotrypsinlike protease from treponema denticola (atcc 29522), and selected bacterial species commonly isolated in periodontitis was studied. the bacteria included were prevotella intermedia (atcc 25261), prevotella buccae (es 57) ...19921398963
immune responses to soluble antigens of treponemes.sonicates from five cultivable treponemes were used as antigens in delayed hypersensitivity tests and macrophage inhibition assays. immunodiffusion analysis showed that the sonicate comprised two major antigenic components which were not distinguishable in the skin tests. the sonicate antigens elicited significant cell-mediated immunity in guinea-pigs. treponema refringens biotype nichols proved to induce the strongest delayed response. delayed skin hypersensitivity to the antigens of treponema ...1977402976
restriction fragment-length polymorphism analysis of 16s ribosomal rna genes amplified by polymerase chain reaction for rapid identification of cultivable oral treponemes.although oral treponemes are among the most frequently found bacteria in periodontal pockets, identification of these organisms can be difficult. in this study, restriction fragment-length polymorphism (rflp) analysis of polymerase chain reaction (pcr)-amplified 16s ribosomal rna genes (16s rrna gene pcr-rflp) was used to generate restriction profiles of reference strains of oral treponemes including treponema denticola, treponema socranskii, treponema vincentii. treponema pectinovorum and trepo ...199910219171
proline iminopeptidase from the outer cell envelope of the human oral spirochete treponema denticola atcc 35405.certain periodontopathic organisms have been shown to exhibit high activity of proline iminopeptidase (pipase). the human oral spirochete treponema denticola atcc 35405 was found to contain an easily extractable, novel pipase (ec, which was purified to a sodium dodecyl sulfate- polyacrylamide gel electrophoresis-pure form by means of fast protein liquid chromatographic procedures. the range of the minimum monomeric molecular mass (280 amino acid residues) of the pipase, based on amino ...19968641769
cloning and expression of hemolysin genes from treponema denticola strains atcc 35404 (td-4) and human clinical isolate gm-1 in escherichia coli.the oral spirochete, treponema denticola is a putative etiologic agent in adult periodontitis, and acute necrotizing ulcerative gingivitis. in vitro, the oral treponeme produces several factors including proteases, hemolysins, hemin-binding proteins, which could potentially be involved in the virulence of this spirochete. our laboratory has been investigating the pathobiology of t. denticola, and has demonstrated the production of several hemolysins by t. denticola. in this report two hemolysin ...19947815917
homology among treponema denticola plasmids.three of 16 isolates of treponema denticola were found to contain small (2.0-2.7 kb) cryptic plasmids. these were ptd1 from t. denticola atcc 33520, ptd2 from strain t32a, and ptd3 from strain d3a1. these plasmids were characterized by restriction mapping and cloned into e. coli plasmid puc19. extensive homology between these plasmids was revealed by southern blot, and single-stranded dna regions were found by neutral southern blots and s1 nuclease mapping. these plasmids were not found in serov ...19947808774
comparison of host tissue and bacterial dipeptidyl peptidases in human gingival crevicular fluid by analytical isoelectric focusing.earlier work has shown that gingival crevicular fluid (gcf) contains dipeptidyl peptidase (dpp) activities that resemble those in host tissue. here, further comparisons were made with enzymes from suspected periodontal pathogens. gingival tissue and gcf were collected from patients with chronic periodontitis. dpp ii and dpp iv fractions with acid and alkaline ph optima, respectively, were separated from crude tissue extracts by gel-filtration chromatography. bacterial cell sonicates were prepare ...19957487574
herpesviral-bacterial synergy in the pathogenesis of human periodontitis.periodontitis is an infectious disease, but the specific mechanisms by which tooth-supportive tissues are lost remain obscure. this article proposes an infectious disease model for periodontitis in which herpesviral-bacterial interactions assume a major etiopathogenic role.200717471038
[nutritional requirements for oral treponema].oral treponema is still difficult to cultivate in vitro because of lacking of our knowledge about what they require for growth. the aim of this study was to find what were the nutritional requirements for them. treponema denticola, t. vincentii, and t. socranskii were cultivated in various broths to seek which fractions of tryptone and rabbit serum, or serum proteins were stimulatory. treponemal growth was assessed by turbidity readings. it was obvious that a weakly negative-charged peptide isol ...19892637426
treponema denticola outer membrane inhibits calcium flux in gingival fibroblasts.treponema denticola is a cultivable oral spirochete which perturbs the cytoskeleton in cultured cells of oral origin, but intracellular signalling pathways by which it affects actin assembly are largely unknown. as the outer membrane (om) of treponema denticola disrupts actin-dependent processes that normally require precise control of intracellular calcium, we studied the effects of an om extract on internal calcium release, ligand-gated and calcium release-activated calcium channels, and relat ...19989453630
scanning and transmission electron microscopy of the phagocytosis of treponema denticola and escherichia coli by human neutrophils in the present in vitro study, scanning and transmission electron microscopy demonstrated that human neutrophils are able to phagocytize treponema denticola cells. two major modes of particle engulfment were detected, both of which seemed unaffected by opsonization or atmosphere (aerobic or anaerobic). the occasional finding of rather intact treponemes as long as 2 h after onset of the phagocytosis experiment, suggested that digestion could be a relatively slow process. expulsion of digested mat ...19846382567
treponema denticola and porphyromonas gingivalis as prognostic markers following periodontal treatment.subgingival plaque samples were collected from individuals with advanced periodontitis before and 3 to 11 weeks after scaling and root planing periodontal treatment. the plaque levels of treponema denticola and porphyromonas gingivalis antigens were measured before and after treatment by a quantitative immunoassay procedure using monoclonal antibodies specific for these oral bacteria. a decrease in mean levels of t. denticola (p less than .05) and p. gingivalis antigens (p less than .09) were ob ...19921315388
inability of intact cells of treponema denticola to degrade human serum proteins iga, igg and albumin.treponema denticola has been shown to be associated with periodontitis in man and animals. the organism ferments amino acids and thrives on the proteins in the periodontal pocket. accordingly, t. denticola possesses various proteolytic enzymes, including a chymotrypsin-like protease, capable of hydrolyzing a whole range of proteins, including immunoglobulins. yet, it is not clear whether the intact cells of t. denticola can degrade immunoglobulins and albumin. the purpose of this study was to cl ...199910412854
the diversity of periodontal spirochetes by 16s rrna analysis.the purpose of this study was to examine the diversity of spirochetes in the subgingival pocket of multiple subjects with a range of periodontal conditions, including two healthy, one adult periodontitis, three acute necrotizing ulcerative gingivitis, eight refractory periodontitis, and one human immunodeficiency virus (hiv) periodontitis. the 16s rrna genes of spirochetes in plaque were amplified by polymerase chain reaction using spirochete selective primers. over 500 clones were sequenced and ...200011154403
porphyromonas gingivalis vesicles enhance attachment, and the leucine-rich repeat bspa protein is required for invasion of epithelial cells by "tannerella forsythia".the human oral cavity harbors more than 500 species of bacteria. periodontitis, a bacterially induced inflammatory disease that leads to tooth loss, is believed to result from infection by a select group of gram-negative periodontopathogens that includes porphyromonas gingivalis, treponema denticola, and "tannerella forsythia" (opinion on name change from tannerella forsythensis pending; formerly bacteroides forsythus). epithelial cell invasion by periodontopathogens is considered to be an impor ...200616926393
interaction of treponema denticola td-4, gm-1, and ms25 with human gingival fibroblasts.the adherence of treponema denticola gm-1, td-4, and ms25 to human gingival fibroblasts (hgfs) was studied to serve as an introduction to investigations into the interactions of these oral bacteria with human host cells. under both aerobic (5% co2) and anaerobic (85% n2 plus 10% h2 plus 5% co2) environments, the interactions with the hgfs were such that strains gm-1 and ms25 were consistently more adherent than strain td-4. polyclonal antibodies to gm-1 inhibited gm-1 adherence by 70%, while ms2 ...19902160430
humoral immune response in early-onset periodontitis: influence of smoking.sixty-five patients with generalised early-onset periodontitis (g-eop) (age range 16-42 years, 32 smokers and 33 non-smokers) were assessed for antibody titres and avidity to a panel of five suspected periodontal pathogens (porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia, treponema denticola and bacteroides forsythus). thirty-four of these patients were untreated (17 smokers and 17 non-smokers), and thirty-one were in the maintenance phase of periodontal the ...200111519695
identification of lactoferrin-binding proteins from treponema pallidum subspecies pallidum and treponema denticola.lactoferrin-binding or -associated proteins were identified in treponema pallidum subspecies pallidum and treponema denticola by affinity column chromatography using human lactoferrin and detergent-solubilized, radiolabelled spirochaetes. two discrete polypeptides of t. pallidum with masses of 45 and 40 kda and a broad band from 29-34 kda exhibited association with human apo- and partially ferrated lactoferrin. t. denticola produced two proteins that associated with a lactoferrin affinity matrix ...19947934885
association of human t lymphotropic virus 1 amplification of periodontitis severity with altered cytokine expression in response to a standard periodontopathogen infection.periodontal diseases (pds) are infectious diseases in which periodontopathogens trigger chronic inflammatory and immune responses that lead to tissue destruction. recently, viruses have been implicated in the pathogenesis of pds. individuals infected with human t lymphotropic virus 1 (htlv-1) present with abnormal oral health and a marked increased prevalence of periodontal disease.201020038241
involvement of periodontopathic anaerobes in aspiration pneumonia.increasing evidence has linked the anaerobic bacteria forming periodontopathic biofilms with aspiration pneumonia in elderly persons. in experiments designed to eliminate the potent respiratory pathogens forming biofilms in the oral cavity, we have shown that the mechanical and chemical oral cleansing using povidone-iodine effectively reduced the detection rates and numbers of methicillin-sensitive staphylococcus species, streptococcus pneumoniae, and haemophilus influenzae in patients scheduled ...200516277588
[polymorphism of il1α and il1β genes and bacterial invasion in patients with chronic generalized periodontitis].researchers studied polymorphism of il-1α and il-1β genes among patients of different ages with severe chronic generalized periodontitis and people with healthy periodontium. the study demonstrated a correlation between high frequency of genetic polymorphism in il-1α -899 and il-1β +3953 loci and development of severe periodontitis among patients less than 35 years of age, as well as markers of p. intermedia, t. denticola, hsv1 and ebv in periodontal pocket.201021311438
sulfhemoglobin formation in human erythrocytes by cystalysin, an l-cysteine desulfhydrase from treponema denticola.cystalysin, isolated from the oral pathogen treponema denticola, is an l-cysteine desulfhydrase (producing pyruvate, ammonia and hydrogen sulfide from cysteine) that can modify hemoglobin and has hemolytic activity. here, we show that enzymatic activity of recombinant cystalysin depends upon stochiometric pyridoxal phosphate. the enzyme was not functional as an l-alanine transaminase, and had a strong preference for l-cysteine over d-cysteine. cystalysin preferred small alpha-l-amino acids as su ...199910495709
taxonomy and virulence of oral spirochetes.all oral spirochetes are classified in the genus treponema. this genus is in the family spirochaetaceae as in bergey's manual of systematic bacteriology. other generic members of the family include spirochaeta, cristispira and borrelia. this conventional classification is in accord with phylogenetic analysis of the spirochetes based on 16s rrna cataloguing. the oral spirochetes fall naturally within the grouping of treponema. only four species of treponema have been cultivated and maintained rel ...200011155157
[collagen-binding proteins of human oral spirochetes].to evaluate pathogenic effect of the collagen-binding proteins(cbp) of human oral spirochetes.199911776931
treponema denticola induces interleukin-8 and macrophage chemoattractant protein 1 production in human umbilical vein epithelial cells.treponema denticola, a major pathogen of periodontitis, has also been detected in the lesions of atherosclerosis. the aim of this study was to investigate induction of chemokine production in human umbilical vein endothelial cells (huvecs) by t. denticola and determine whether those chemokines were degraded by a protease, dentilisin. t. denticola atcc35405 or dentilisin-deficient mutant k1 were added to huvecs and levels of interleukin-8 (il-8) and monocyte chemoattractant protein-1 (mcp-1) in t ...200717533151
establishment of human indigenous bacteria in germ-free mice.gibbons, r. j. (forsyth dental center and harvard school of dental medicine, boston, mass.), s. s. socransky, and b. kapsimalis. establishment of human indigenous bacteria in germ-free mice. j. bacteriol. 88:1316-1323. 1964.-thirteen strains of bacteria indigenous to the gingival crevice area of man were tested for their ability to establish as monocontaminants in germ-free mice. three facultative organisms, streptococcus mitis, staphylococcus albus, and a "diphtheroid," established, as well as ...196414234787
an actin-stabilizing peptide conjugate deduced from the major outer sheath protein of the bacterium treponema denticola.a synthetic peptide conjugated to bovine serum albumin, p34(bsa), based on a 10-mer in the deduced amino acid sequence of the major outer sheath protein of treponema denticola, was found to stabilize actin filaments of fibroblasts. pretreatment of cells with p34(bsa) inhibited the actin disruption induced by cytochalasin d and latrunculin b. p34(bsa) was taken up by the cells and localized among actin filaments. p34(bsa) bound actin from fibroblast lysates, and cell exposure to p34(bsa) led to t ...200717565753
tannerella forsythia and the hla-dqb1 allele are associated with susceptibility to periodontal disease in japanese adolescents.periodontal disease is a multiple factor disease caused by genetic factors, environmental factors, and periodontal bacteria (periodontal pathogens). the present study aimed to elucidate the risk factors for periodontal disease in japanese adolescents. subjects (11-16 years old) were classified into three groups: localized aggressive periodontitis (lap), periodontal attachment loss (pal), and periodontally healthy (ph) groups. genomic dna isolated from the buccal mucosa was used for single-nucleo ...200919184295
treponema denticola immunoinhibitory protein induces irreversible g1 arrest in activated human lymphocytes.oral spirochetes may contribute to the pathogenesis of a number of disorders including periodontal and periradicular diseases; however, the mechanism (s) by which these organisms act to cause disease is unknown. we have previously shown that extracts of the oral spirochete, treponema denticola, contain an immunosuppressive protein (sip) which impairs human lymphocyte proliferation. the objective of this study was to determine the mechanism by which sip alters the proliferative response of lympho ...200415107064
modeling bacterial damage to pulpal cells in vitro.there is increasing evidence that access to patent dentinal tubules by bacteria and their products rather than trauma from restorative materials is responsible for subsequent pulpitides. the purpose of this study was to compare the relative cytotoxicity of centrifugal fractions of two bacteria, fusobacterium nucleatum and treponema denticola, on l929 cells in monolayer cultures and in the "in vitro pulp chamber." neutrophilic chemotaxis assays and limmulus assays were performed to verify biologi ...19911895035
identification of early microbial colonizers in human dental elucidate the first colonizers within in vivo dental biofilm and to establish potential population shifts that occur during the early phases of biofilm formation.200415546422
molecular analysis of human oral microbiota.the application of molecular, mainly 16s ribosomal rna (rrna)-based approaches enables researchers to bypass the cultivation step and has proven its usefulness in studying the microbial composition in a variety of ecosystems, including the human oral cavity. in this mini-review, we describe the impact of these culture-independent approaches on our knowledge of the ecology of the human oral cavity and provide directions for future studies that should emphasize the role of specific strains, specie ...200515853975
genetic relatedness and phenotypic characteristics of treponema associated with human periodontal tissues and ruminant foot disease.treponema have been implicated recently in the pathogenesis of digital dermatitis (dd) and contagious ovine digital dermatitis (codd) that are infectious diseases of bovine and ovine foot tissues, respectively. previous analyses of treponemal 16s rdna sequences, pcr-amplified directly from dd or codd lesions, have suggested relatedness of animal treponema to some human oral treponema species isolated from periodontal tissues. in this study a range of adhesion and virulence-related properties of ...200312724370
hydrogen sulfide induces apoptosis in human periodontium cells.the existence of hydrogen sulfide (h(2)s) at high concentrations in periodontal pockets is a characteristic feature of periodontitis. periodontal pathogens play a key role in the production of h(2)s under these etiology conditions. this study was designed to examine the cytotoxicity of h(2)s in periodontium cells, including human periodontal ligament (pdl) cells and human gingival fibroblasts (hgfs), as well as the role of h(2)s in apoptosis induction.201019602114
periodontitis may increase the risk of peripheral arterial disease.the aim of this case control study was to evaluate whether periodontitis was associated with peripheral arterial disease (pad).200817964192
treponema denticola lipooligosaccharide activates gingival fibroblasts and upregulates inflammatory mediator response to bacterial challenges, fibroblasts, a major constituent of gingival connective tissue, can produce immunoregulatory cytokines and proteolytic enzymes that may contribute to tissue destruction and the progression of periodontitis, a chronic inflammatory disease affecting tooth-supporting tissues, including alveolar bone. the spirochete treponema denticola is a major etiological agent of periodontitis and can invade oral tissues. the aim of the present study was to investigate the in ...200818366071
streptococci dominate the diverse flora within buccal cells.previously, we reported that intracellular actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. in this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify fusobacterium nucleatum, prevotella intermedia, oral campylobacter species, eikenella corrodens, treponema denticola, gemella haemo ...200516304448
evidence of periopathogenic microorganisms in placentas of women with preeclampsia.periodontal disease is a chronic inflammatory infectious disease that may act as a focus of infection. preeclampsia is a pregnancy-specific hypertensive disorder that often leads to maternal morbidity and mortality. acute atherosis, the placental lesion of preeclampsia, shares many histopathological features with atherosclerosis. recently, chronic infection was linked to the initiation of atherosclerosis. oral pathogens have been detected in atherosclerotic plaques, where they may play a role in ...200717397314
supernatants from oral epithelial cells and gingival fibroblasts modulate human immunodeficiency virus type 1 promoter activation induced by periodontopathogens in monocytes/macrophages.bacterial and host cell products during coinfections of human immunodeficiency virus type 1-positive (hiv-1(+)) patients regulate hiv-1 recrudescence in latently infected cells (e.g. t cells, monocytes/macrophages), impacting highly active antiretroviral therapy (haart) failure and progression of acquired immunodeficiency syndrome. a high frequency of oral opportunistic infections (e.g. periodontitis) in hiv-1(+) patients has been demonstrated; however, their potential to impact hiv-1 exacerbati ...201020331801
gingival tissue transcriptomes in experimental gingivitis.jönsson d, ramberg p, demmer rt, kebschull m, dahlén g, papapanou pn. gingival tissue transcriptomes in experimental gingivitis. j clin periodontol 2011; doi: 10.1111/j.1600-051x.2011.01719.x. abstract: aims: we investigated the sequential gene expression in the gingiva during the induction and resolution of experimental gingivitis. material and methods: twenty periodontally and systemically healthy non-smoking volunteers participated in a 3-week experimental gingivitis protocol, followed by deb ...201121501207
molecular detection of human periodontal pathogens in oral swab specimens from dogs in japan.periodontal diseases are known to be major diseases in humans, and are also common in dogs. the purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. the distribution of an animal gingival organism porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including porphyromonas gingivalis, treponema denticola, tannerella forsythia, capnocytophaga ochracea, capnocyt ...201121916371
granulocyte chemotactic protein 2 (gcp-2/cxcl6) complements interleukin-8 in periodontal disease.mucosal inflammatory responses are orchestrated largely by pro-inflammatory chemokines. the chemokine granulocyte chemotactic protein 2 (cxcl6) is involved in neutrophil recruitment and migration. previous studies have shown that granulocyte chemotactic protein 2 is up-regulated during mucosal inflammation (e.g. in inflammatory bowel disease), similarly to the functionally and structurally related chemokine interleukin-8. nevertheless, unlike interleukin-8, a role of granulocyte chemotactic prot ...200918842116
treponema denticola suppresses expression of human {beta}-defensin-3 in gingival epithelial cells through inhibition of the toll-like receptor 2 axis.we reported previously that treponema denticola, one of the periodontal pathogens, suppresses the expression of human beta-defensins (hbds) in human gingival epithelial cells. to identify the mechanisms involved in this suppression, immortalized and normal human gingival epithelial cells were infected with live or heat-killed t. denticola for 24 h, and then the expression of hbds was examined by real-time rt-pcr. live t. denticola suppressed the expression of hbd-3 substantially and also suppres ...201019995893
inhibition of proteolytic, serpinolytic, and progelatinase-b activation activities of periodontopathogens by doxycycline and the non-antimicrobial chemically modified tetracycline derivatives.tetracyclines, particularly doxycycline (doxy), and their non-antimicrobial chemically-modified derivatives (cmts) inhibit the activities of human matrix metalloproteinases (mmps), and reduce the severity and progression of periodontal disease in animal models and humans. in this study, the effects of doxy and cmt-1, -3, and -5 on proteolytic, serpinolytic, and progelatinase-b activation activities of potent periodontopathogens were studied.200211846203
microbiological, immunological and genetic factors in family members with periodontitis as a manifestation of systemic disease, associated with hematological disorders.the microflora, immunological profiles of host defence functions, and human leukocyte antigen (hla) findings are reported for a mother, son and daughter who were diagnosed as having 'periodontitis as a manifestation of systemic diseases, associated with hematological disorders'. examinations were made of the bacterial flora from the periodontal pocket, neutrophil chemotaxis, neutrophil phagocytosis, and the genotypes (dqb1) and serotypes (dr locus) of hla class ii antigens. phenotypic analyses o ...200212200976
genetic manipulation of treponema denticola.the oral anaerobic spirochete, treponema denticola, has been implicated in the etiology of human periodontal diseases; however, the molecular basis for the virulence of these organisms is still unclear. potential pathogenic factors expressed by t. denticola have recently begun to be identified through the development of gene transfer approaches in this organism following electroporetic transformation. several antibiotic resistance markers have been developed for use in the construction of monosp ...200518770552
lesion formation and antibody response induced by papillomatous digital dermatitis-associated spirochetes in a murine abscess model.papillomatous digital dermatitis (pdd), also known as hairy heel wart, is a growing cause of lameness of cows in the u.s. dairy industry. farms with pdd-afflicted cows experience economic loss due to treatment costs, decreased milk production, lower reproductive efficiency, and premature culling. while the exact cause of pdd is unknown, lesion development is associated with the presence of anaerobic spirochetes. this study was undertaken to investigate the virulence and antigenic relatedness of ...200717591787
toll-like receptors 2 and 5 in human gingival epithelial cells co-operate with t-cell cytokine interleukin-17.periodontitis begins as the result of perturbation of the gingival epithelial cells caused by subgingival bacteria interacting with the epithelial cells via pattern recognition receptors. toll-like receptors (tlrs) have been shown to play an important role in the recognition of periodontal pathogens so we have studied the interaction of tlr ligands with tlr2 and tlr5 for cytokine production in the cultures of gingival epithelial cells.200919121068
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