Publications

TitleAbstractYear
Filter
PMID
Filter
the effect of the ovine host parasitaemia on the development of babesia ovis (babes, 1892) in the tick rhipicephalus bursa (canestrini and fanzago, 1877).batches of rhipicephalus bursa adult ticks were fed on two lambs with 10.0% (batch 1) and 0.3% (batch 2) babesia ovis parasitaemia, respectively. haemolymph and eggs were checked for parasites daily after detachment, before and after appearance of b. ovis in the lamb's blood.b. ovis kinetes were found in the haemolymph and eggs earlier in the engorged ticks detached before appearance of the parasite in the host blood. rates of haemolymph and egg infection with b. ovis as well as the percentage o ...200111240093
clinical, clinico-pathological and serological studies of babesia ovis in experimentally infected sheep.clinical, clinico-pathological and serological studies were performed in sheep experimentally infected with babesia ovis. acute babesiosis occurred in all the lambs infested with adult rhipicephalus bursa ticks and in one lamb infested with the larvae. the rate of parasitaemia and the degree of anaemia were not correlated. decrease in the packed-cell volume ranged from 30 to 40%. parasitized erythrocytes were not observed to block capillaries in the brain, which explained the absence of nervous ...19989780825
[ultrastructure and cytochemistry of the pellicle and apical complexes of the kinete of babesia bigemina and babesia ovis in the hemolymph and oavry of the tick].the term kinete is used in this paper for the cigar-shaped, motile development stages (vermicule") of babesia occurring intra- and extracellularly in hemolymph and overy (including oocytes) of vectors, hard ticks (ixodoidea). the structure of, and cytochemical activities of hydrolases (acid phosphatase, nonspecific esterase) in the pellicle and the apical complex was studied at the fine-structural level in kinetes of babesia bigemina smith & kilborne, in hemolympho of female boophilus microplus ...19807373571
ultrastructural study of the development of babesia ovis (piroplasmia) in the ovary of the vector tick rhipicephalus bursa.the reproduction of babesia ovis inside the ovary of the vector tick rhipicephalus bursa was studied by electron microscopy. the kinetics of b. ovis invade the ovary of the tick via hemolymph. the kinete, when situated intracellularly, is transformed into a polymorphous stage that loses all features of the invasive form. the nucleus elongates enormously and forms many folds which extend throughout the cell. subsequently, the lobulated nucleus and the cytoplasm are divided into numerous uninuclea ...19827086711
ultrastructural demonstration of succinic dehydrogenase and cytochrome oxidase activity in sporozoites of babesia ovis and theileria annulata (apicomplexa: piroplasmea) in salivary glands of tick vectors (rhipicephalus bursa, hyalomma anatolicum excavatum).salivary gland stages ("sporozoites") of babesia ovis and theileria annulata (apicomplexa: piroplasmea) in female ixodid ticks were studied for ultracytochemical activity of the respiratory enzymes, succinic dehydrogenase (sdh), and cytochrome oxidase. both sdh and cytochrome oxidase were demonstrated in the sporozoites and the mitochondria in these stages. identified in this way the final reaction product of sdh was located mainly at the inner side of the mitochondrial boundary, though it was a ...19806260921
electron microscopic study on the development of babesia ovis (piroplasmia) in the salivary glands of the vector tick rhipicephalus bursa.the formation of babesia ovis sporozoites in salivary gland cells of the vector tick rhipicephalus bursa was studied by electron microscopy. the kinetics of b. ovis were found lying intracellularly on the second day after infestation (a.i.) of the ticks. the parasites enlarged rapidly losing all features of the motile form. invaginations of the cell membrane initiated a fragmentation of this developmental stage. on the third day a.i the parasite (measuring 40 x 25 microns) was divided into numer ...19826122360
general review of tick species present in portugal.at present, 24 species are known to occur in portugal: argas vespertilionis, ornithodoros maritimus and ornithodoros erraticus in argasidae; ixodes acuminatus, ixodes bivari, ixodes canisuga, ixodes frontalis, ixodes hexagonus, ixodes ricinus, ixodes simplex simplex, ixodes ventalloi, ixodes vespertilionis, dermacentor marginatus, dermacentor pictus', haemaphysalis hispanica, haemaphysalis inermis, haemaphysalis punctata, rhipicephalus bursa, rhipicephalus pusillus, rhipicephalus sanguineus, rhi ...199911071535
quantitative description of the development of babesia ovis in rhipicephalus bursa (hemolymph, ovary, eggs).the development and infection dynamics of babesia ovis in the hemolymph, ovaries, and eggs of rhipicephalus bursa are described quantitatively, based mainly on examination of giemsa-stained smears. after alimentary infection of female ticks, their hemolymph became infected 5 days after repletion (p.repl.). the prevalence and mean intensity of infection increased during the course of infection studied, up to 17 days p.repl. after vertical infection of female ticks, their hemolymph was infected on ...19883387406
[fine structure of babesia ovis (piroplasmidea) in rhipicephalus bursa (ixodoidea): transformation from spheroid to vermicule form]. 19684988934
determination of prevalence and risk factors of infection with babesia ovis in small ruminants from west azerbaijan province, iran by polymerase chain reaction.small ruminants' babesiosis caused by babesia ovis, is transmitted during blood feeding by infected ticks and is the most economically important tick-borne disease in tropical and subtropical areas. this study was carried out to to estimate the infection rate of b. ovis in sheep and goats by pcr. we have analysed risk factors that might influence infection of sheep and goats with b. ovis.201526623436
[microscopic studies on the development of babesia ovis (piroplasmidea) in rhipicephalus bursa (ixoidea). i. the development in female ticks following repletion]. 19694988937
molecular identification of theileria and babesia in ticks collected from sheep and goats in the black sea region of turkey.a molecular survey was undertaken in the black sea region of turkey to determine the presence of theileria and babesia species of medical and veterinary importance. the ticks were removed from sheep and goats, pooled according to species and locations, and analyzed by pcr-based reverse line blot (rlb) and sequencing. a total of 2241 ixodid ticks belonging to 5 genus and 12 species were collected and divided into 310 pools. infection rates were calculated as the maximum likelihood estimation (mle ...201525260692
pcr-based detection of babesia ovis in rhipicephalus bursa and small ruminants.this study aimed to assess the prevalence of babesia ovis infection in adult rhipicephalus bursa and small ruminants in west azerbaijan province, iran. blood samples were collected from 280 sheep and 122 goats of forty randomly selected flocks. specific b. ovis fragment was detected in 67 animals (16.7%), of which 52 animals (18.6%) were sheep and 15 animals (12.2%) goats (p < 0.05). of the 848 r. bursa collected from naturally infested small ruminants and farm dogs, babesia ovis was detected by ...201424876944
molecular detection of babesia ovis in sheep and ticks using the gene encoding b. ovis surface protein d (bospd).the gene encoding babesia ovis surface protein d (bospd) was cloned from b. ovis cdna library. this gene encodes a polypeptide chain of 155 amino acids, including a predicted 22 amino acid signal peptide. sequence analysis of the bospd suggested that it is a surface protein with no known domains. blast analysis followed by multiple alignments showed four orthologs from other apicomplexan species and suggested that bospd is specific for b. ovis. bospd-based pcr was then developed to specifically ...201526391822
quantitative analysis of babesia ovis infection in sheep and ticks.a quantitative pcr, based on the gene encoding babesia ovis surface protein d (bospd) was developed and applied to investigate the presence of babesia ovis (b. ovis) in its principal vector, the tick rhipicephalus bursa (r. bursa), and in the ovine host. quantification of b. ovis in experimentally-infected lambs showed a sharp increase in parasitemia 10-11 days in blood-inoculated and adult tick-infested lambs, and 24 days in a larvae-infested lamb. a gradual decrease of parasitemia was observed ...201627084469
transmission of babesia ovis by different rhipicephalus bursa developmental stages and infected blood injection.in this report, the transmission efficacy of babesia ovis, the principal causative agent of ovine babesiosis, was studied by infestation of lambs with different rhipicephalus bursa stages or by injection of infected blood. infected blood injection induced acute babesiosis in splenectomized lambs, while only mild clinical signs were observed in intact animals. both splenectomized and intact lambs developed high antibody titer, detectable for at least 180 days post infection. infestation of splene ...201626253782
tick-borne diseases in ruminants of central and southern italy: epidemiology and case reports.sera and blood from cattle and sheep were examined for the presence of babesia and theileria spp by microscopy and serology at the parasitology department of the istituto zooprofilattico sperimentale of abruzzo and molise (izsam). of the 47 bovine herds (323 animals) tested, 15 were found positive for babesia bigemina and 1 for babesia bovis. two outbreaks occurred, one caused by b. bigemina and one by b. bovis. the b. bigemina outbreak occurred in abruzzo and has been followed for two years. th ...199911071553
seroprevalence of babesia ovis in sheep in catalonia, northeastern spain.an indirect fluorescent antibody test (ifat) was developed in order to detect antibodies against babesia ovis in different sheep flocks from catalonia, northeastern spain. of the total 2174 sera included in the study, 133 (6.1%) were positive. considerable differences in seroprevalence were detected depending on the geographical areas from which the animals originated. this must have been a consequence of the presence or absence of the vector tick, rhipicephalus bursa, the distribution of which ...19989831951
some epizootiological and clinical aspects of ovine babesiosis caused by babesia ovis--a review.the study shows a close relationship between incidence of ovine babesiosis caused by babesia ovis and the activity period and distribution area of the vector tick rhipicephalus bursa. the most important factor limiting the distribution of this tick is a decrease in humidity. in general, it is absent from areas with an average annual rainfall of less than about 300 mm. the rate of parasitaemia and the degree of anaemia were not correlated. decrease of the packed-cell volume ranged from 30 to 40%. ...19989561703
detection of babesia ovis by pcr in rhipicephalus bursa collected from naturally infested sheep and goats.polymerase chain reaction (pcr) was used to assess the presence and the frequency of babesia ovis infection in the adult rhipicephalus bursa and their hosts in elazig province located in eastern turkey. tick and blood samples were collected from 32 sheep and 28 goats of four selected herds. a total of 226 r. bursa were randomly selected from the collected ticks and their salivary glands were dissected out in 0.85% saline under stereo microscope. dna amplification method revealed that the frequen ...200817881019
molecular detection of theileria, babesia, and hepatozoon spp. in ixodid ticks from palestine.ixodid ticks transmit various infectious agents that cause disease in humans and livestock worldwide. a cross-sectional survey on the presence of protozoan pathogens in ticks was carried out to assess the impact of tick-borne protozoa on domestic animals in palestine. ticks were collected from herds with sheep, goats and dogs in different geographic districts and their species were determined using morphological keys. the presence of piroplasms and hepatozoon spp. was determined by pcr amplifica ...201626969489
molecular detection of theileria spp. and babesia spp. in sheep and ixodid ticks from the northeast of iran.theilerioses and babesioses are important diseases in iranian sheep. the present study was undertaken to identify and classify/specify theileria spp. and babesia spp. in sheep and vector ticks. investigation was carried out from 2009 to 2011 in the khorasan razavi province, iran. in total, 302 sheep originating from 60 different flocks were clinically examined and their blood collected. in addition, from the same flocks, ixodid ticks were sampled. stained blood smears were microscopically examin ...201322924924
theileria annulata and babesia ovis: ultracytochemical lactic dehydrogenase activity of sporozoites in salivary glands of female ticks, hyalomma anatolicum excavatum and rhipicephalus bursa. 19826806115
a study of an enzootic focus of sheep babesiosis (babesia ovis, babes, 1892).morbidity and mortality due to babesia ovis in sheep flocks grazing in an enzootic area of israel occur yearly, about 2 weeks after detection of adult rhipicephalus bursa ticks on the animals. disease incidence peaks in may, but lasts throughout the active period of the adult ticks in the spring-summer months of april-july. no clinical cases of babesiosis have been registered during the active period of the preimaginal stages of r. bursa, from october to february. incidence of parasitaemia durin ...19958747918
[light microscopy studies on the development of babesia ovis (piroplasmidea) in rhipicephalus bursa (ixodoidea). ii. cytochemical studies of differentiated merozoites in the salivrry glands of female ticks]. 19714994823
molecular and parasitological survey of ovine piroplasmosis, including the first report of theileria annulata (apicomplexa: theileridae) in sheep and goats from turkey.blood and tick samples were collected from 333 apparently healthy sheep and 257 goats as well as 10 sheep exhibiting clinical signs of babesiosis in adana, gaziantep, and adiyaman provinces in southern turkey. fully engorged female ticks were selected and maintained in an incubator until they oviposited. the tick carcasses and their egg masses were examined. piroplasms compatible with babesia spp. and theileria spp. were observed in both symptomatic and asymptomatic small ruminants. genomic dna ...201728082649
molecular evidence of tick-borne hemoprotozoan-parasites (theileria ovis and babesia ovis) and bacteria in ticks and blood from small ruminants in northern algeria.using qpcr, standard pcr and/or sequencing, we investigated the presence of tick-associated microorganisms in ticks and blood from sheep and goats from souk ahras, algeria. borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. anaplasma ovis was screened in (38/73, 52%) rhipicephalus bursa and (5/22, 22.7%) r. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively. coxiella burnetii tested positive in r. bursa (4/73, 5.5%) a ...201728131376
a survey of ixodid tick species and molecular identification of tick-borne pathogens.this study was undertaken in two different climatic areas of turkey to determine the presence of tick-borne pathogens of medical and veterinary importance. the ticks were removed from humans, pooled according to species and developmental stages, and analyzed by pcr, reverse line blot (rlb) and sequencing. of the 2333 removed ticks from 10 species, 1238 (53.06%) were obtained from the arid cold zone, and the remaining 1095 (46.93%) were obtained from the humid zone. the removed ticks were identif ...201424424312
Displaying items 1 - 28 of 28