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actinobacillus rossii sp. nov., actinobacillus seminis sp. nov., nom. rev., pasteurella bettii sp. nov., pasteurella lymphangitidis sp. nov., pasteurella mairi sp. nov., and pasteurella trehalosi sp. nov.evidence from numerical taxonomic analysis and dna-dna hybridization supports the proposal of new species in the genera actinobacillus and pasteurella. the following new species are proposed: actinobacillus rossii sp. nov., from the vaginas of postparturient sows; actinobacillus seminis sp. nov., nom. rev., associated with epididymitis of sheep; pasteurella bettii sp. nov., associated with human bartholin gland abscess and finger infections; pasteurella lymphangitidis sp. nov. (the blg group), w ...19902223608
divergent activity and function of superoxide dismutases in pasteurella haemolytica serotypes a1 and a2 and pasteurella trehalosi serotype t10.representative strains of pasteurella haemolytica serotypes a1 and a2 and pasteurella trehalosi serotype t10 were examined for the presence of superoxide dismutase. visualisation of superoxide dismutase enzyme activity on polyacrylamide gels, and specific inhibition with potassium cyanide verified a copper/zinc (cu/zn) superoxide dismutase only in serotype a2 whereas serotypes a1 and t10 showed other superoxide dismutase activity. using a simple freeze-thaw method the cellular location of supero ...19979170262
genetic relationships among pasteurella trehalosi isolates based on multilocus enzyme electrophoresis.genetic diversity among 60 british pasteurella trehalosi isolates representing the four recognized capsular serotypes, t3, t4, t10 and t15, and recovered predominantly from sheep suffering from systemic pasteurellosis, was estimated by analysing electrophoretically demonstrable allelic variation at structural genes encoding 19 enzymes. thirteen of the locl were polymorphic and 20 distinctive multilocus genotypes (electrophoretic types, ets) were identified. the population structure of p. trehalo ...19979274038
polymerase chain reaction techniques for differentiating cytotoxic and noncytotoxic pasteurella trehalosi from rocky mountain bighorn sheep.to evaluate 2 polymerase chain reaction (pcr)-based methods for differentiating cytotoxic and noncytotoxic pasteurella trehalosi from rocky mountain bighorn sheep (ovis canadensis canadensis).199910328428
serotypes of pasteurella haemolytica and pasteurella trehalosi isolated from farm animals in hungary.the biochemical and serological characteristics of 486 p. haemolytica and 31 p. trehalosi strains (517 in total) isolated from different lesions of cattle, sheep, goats, pigs and poultry were examined. a total of 476 p. haemolytica strains (97.9%) showed the characteristics typical of the former biotype a of p. haemolytica, while 10 isolates (2.1%), all from poultry, could not be biotyped. a total of 481 strains (93.0%) could be assigned to one of the 17 serotypes of p. haemolytica-p. trehalosi ...199910379234
conservation of expression and n-terminal sequences of the pasteurella haemolytica 31-kilodalton and pasteurella trehalosi 29-kilodalton periplasmic iron-regulated proteins.this study examined the conservation of expression of a 31-kda iron-regulated protein by serotypes of pasteurella haemolytica and pasteurella trehalosi associated with pasteurellosis of cattle and sheep. a polyclonal antibody prepared against the purified 31-kda periplasmic iron-regulated protein from p. haemolytica serotype a1 showed that all p. haemolytica serotypes expressed similar 31-kda proteins with identical n-terminal sequences, whereas p. trehalosi serotypes expressed immunologically d ...199910391874
sequence diversity and molecular evolution of the leukotoxin (lkta) gene in bovine and ovine strains of mannheimia (pasteurella) haemolytica.the molecular evolution of the leukotoxin structural gene (lkta) of mannheimia (pasteurella) haemolytica was investigated by nucleotide sequence comparison of lkta in 31 bovine and ovine strains representing the various evolutionary lineages and serotypes of the species. eight major allelic variants (1.4 to 15.7% nucleotide divergence) were identified; these have mosaic structures of varying degrees of complexity reflecting a history of horizontal gene transfer and extensive intragenic recombina ...200111157953
mannheimia haemolytica serotype 1 and pasteurella trehalosi serotype 10 culture supernatants contain fibrinogen-binding proteins.fibrinogen-binding proteins were found in the culture supernatants of mannheimia haemolytica serotype 1 (atcc 43270) and pasteurella trehalosi serotype 10 (eco-100). sheep fibrinogen was biotinylated and shown to bind to proteins in the culture supernatants by modified western blot. fibrinogen-binding proteins in the culture supernatant may be important virulence factors leading to the characteristic fibrinous pneumonia caused by these organisms and may be critical antigenic targets for immune p ...200212406660
pcr-detection of hemophilus paragallinarum, hemophilus somnus, mannheimia (pasteurella) hemolytica, mannheimia spp., pasteurella trehalosi, and pasteurella multocida. 200312512370
diversity of mannheimia haemolytica and pasteurella trehalosi serotypes from apparently healthy sheep and abattoir specimens in the highlands of wollo, north east ethiopia.the prevalence and serotypic diversity of mannheimia [pasteurella] haemolytica and pasteurella trehalosi from nasal swabs, sera and abattoir specimens from sheep in the highlands of wollo, north east ethiopia was investigated. prevalence rates of 83% and 75% of these microorganisms were found in the serum samples and nasal swabs, respectively, from apparently healthy sheep. in a local abattoir, 205 lungs were investigated, 34% of which showed pneumonia, from which samples were collected from 51 ...200312625399
novel protease produced by a pasteurella trehalosi serotype 10 isolate from a pneumonic bighorn sheep: characteristics and potential relevance to protection.a strain of pasteurella trehalosi serotype 10, e(co)-100, isolated from a bighorn sheep that had succumbed to pneumonic pasteurellosis during an epizootic, was compared to well-characterized strains of p. trehalosi serotype 10 and mannheimia haemolytica serotype 1. the gene for leukotoxin a (lkta) from e(co)-100 was sequenced and found to be identical on an amino acid basis to a published sequence for lkta from p. trehalosi serotype 10. however, the toxic activity in culture supernatant measured ...200312637002
sequence diversity and molecular evolution of the heat-modifiable outer membrane protein gene (ompa) of mannheimia(pasteurella) haemolytica, mannheimia glucosida, and pasteurella trehalosi.the ompa (or heat-modifiable) protein is a major structural component of the outer membranes of gram-negative bacteria. the protein contains eight membrane-traversing beta-strands and four surface-exposed loops. the genetic diversity and molecular evolution of ompa were investigated in 31 mannheimia (pasteurella) haemolytica, 6 mannheimia glucosida, and 4 pasteurella trehalosi strains by comparative nucleotide sequence analysis. the ompa proteins of m. haemolytica and m. glucosida contain four h ...200415317779
phylogenetic diversity of pasteurellaceae and horizontal gene transfer of leukotoxin in wild and domestic sheep.wild and domestic animal populations are known to be sources and reservoirs of emerging diseases. there is also a growing recognition that horizontal genetic transfer (hgt) plays an important role in bacterial pathogenesis. we used molecular phylogenetic methods to assess diversity and cross-transmission rates of pasteurellaceae bacteria in populations of bighorn sheep, dall's sheep, domestic sheep and domestic goats. members of the pasteurellaceae cause an array of deadly illnesses including ba ...200716635591
diversity of temperate bacteriophages induced in bovine and ovine mannheimia haemolytica isolates and identification of a new p2-like phage.the diversity of temperate bacteriophages was examined in 32 mannheimia haemolytica, six mannheimia glucosida and four pasteurella trehalosi isolates. phage particles were induced and identified by electron microscopy in 24 (75%) m. haemolytica isolates, but in only one (17%) m. glucosida and one (25%) p. trehalosi isolate. the m. haemolytica phages were relatively diverse as seven siphoviridae, 15 myoviridae and two podoviridae-like phages were identified; the myoviridae-type phages also exhibi ...200616842340
reclassification of [pasteurella] trehalosi as bibersteinia trehalosi gen. nov., comb. nov.[pasteurella] trehalosi is an important pathogen of sheep, being primarily associated with serious systemic infections in lambs but also having an association with pneumonia. the aim of the present investigation was to characterize a broad collection of strains tentatively identified as [p.] trehalosi in order to reclassify and rename this taxon to support improvements in our understanding of the pathogenesis and epidemiology of this important organism. the type strain for [p.] trehalosi, strain ...200717392184
effect of vaccinating lambs against pneumonic pasteurellosis under new zealand field conditions on their weight gain and pneumonic lung lesions at slaughter.in a field trial, 9174 lambs from seven commercial sheep flocks with a history of subclinical pneumonia were either vaccinated with ovipast plus (intervet) or given a placebo by systematic random allocation; they were vaccinated twice at an interval of four to six weeks and grazed on pasture in the same paddocks. they were weighed at the first vaccination, 11 and 23 weeks later, and one to three days before they were slaughtered. the extent of the pneumonic lesions in their lungs was scored visu ...200818178931
microorganisms associated with a pneumonic epizootic in rocky mountain bighorn sheep (ovis canadensis canadensis).a comprehensive study of a pneumonic epizootic was initiated when the first signs of disease were noted in a metapopulation of bighorn sheep inhabiting hells canyon, bordering idaho, oregon, and washington. a total of 92 bighorn sheep were tested for etiologic agents during the following 6-mo study period. the study population included bighorn sheep believed to be the subpopulation in which disease was first noted, and these sheep were translocated to a holding facility in an effort to contain t ...200718229860
variation in pasteurella (bibersteinia) and mannheimia spp. following transport and antibiotic treatment in free-ranging and captive rocky mountain bighorn sheep (ovis canadensis canadensis).morbidity and mortality associated with respiratory disease following capture and translocation of bighorn sheep (ovis canadensis canadensis) is a significant concern, particularly when establishing new or augmenting existing bighorn populations. administration of prophylactic antibiotics at the time of capture is often done to minimize the risk of respiratory disease, but the efficacy of this practice is unknown. the effects of oxytetracycline and florfenicol on the pasteurella (bibersteinia) a ...200919368250
an ecologic study comparing distribution of pasteurella trehalosi and mannheimia haemolytica between sierra nevada bighorn sheep, white mountain bighorn sheep, and domestic sheep.the prevalence and phenotypic variability of pasteurella and mannheimia isolates from sierra nevada bighorn sheep (ovis canadensis sierrae), white mountain bighorn sheep (ovis canadensis nelsoni), and domestic sheep (ovis aries) from california, usa, were compared. the white mountain bighorn sheep population had a recent history of pneumonia-associated mortality, whereas the sierra nevada bighorn sheep population had no recent history of pneumonia-associated mortality. the domestic sheep flocks ...200919901369
bibersteinia trehalosi inhibits the growth of mannheimia haemolytica by a proximity-dependent mechanism.mannheimia (pasteurella) haemolytica is the only pathogen that consistently causes severe bronchopneumonia and rapid death of bighorn sheep (bhs; ovis canadensis) under experimental conditions. paradoxically, bibersteinia (pasteurella) trehalosi and pasteurella multocida have been isolated from bhs pneumonic lungs much more frequently than m. haemolytica. these observations suggest that there may be an interaction between these bacteria, and we hypothesized that b. trehalosi overgrows or otherwi ...201020038698
a bighorn sheep die-off in southern colorado involving a pasteurellaceae strain that may have originated from syntopic cattle.we investigated a pasteurellosis epizootic in free-ranging bighorn sheep (ovis canadensis) wherein a pasteurellaceae strain carried by syntopic cattle (bos taurus) under severe winter conditions appeared to contribute to pneumonia in affected bighorns. twenty-one moribund or dead bighorn sheep were found on the "fossil ridge" herd's winter range, colorado, usa, between 13 december 2007 and 29 february 2008. eight carcasses examined showed gross or microscopic evidence of acute to subacute fibrin ...201020966277
a multivalent mannheimia/bibersteinia vaccine protects bighorn sheep against mannheimia haemolytica challenge.bighorn sheep (bhs) are more susceptible than domestic sheep (ds) to mannheimia haemolytica pneumonia. although both species carry m. haemolytica as a commensal bacterium in the nasopharynx, ds carry mostly leukotoxin (lkt)-positive strains while bhs carry lkt-negative strains. consequently, antibodies to surface antigens and lkt are present at much higher titers in ds, but lower in bhs. the objective of this study was to determine whether repeated immunization of bhs with multivalent mannheimia ...201121832104
domestic sheep (ovis aries) pasteurellaceae isolates from diagnostic submissions to the caine veterinary teaching center (1990-2004).a retrospective study of pasteurellaceae isolated from domestic sheep (ovis aries) was conducted. the aim was to identify pasteurellaceae present in animals that were clinically healthy and others with evidence of respiratory disease. the bacteria had been isolated from samples submitted to the university of idaho caine veterinary teaching center as part of disease diagnostic testing. the 844 isolates identified mainly three species of pasteurellaceae: mannheimia haemolytica, pasteurella multoci ...201121345625
evidence for a common gene pool and frequent recombinational exchange of the tbpba operon in mannheimia haemolytica, mannheimia glucosida and bibersteinia trehalosi.the tbpba operon was sequenced in 42 representative isolates of mannheimia haemolytica (32), mannheimia glucosida (6) and bibersteinia trehalosi (4). a total of 27 tbpb and 20 tbpa alleles were identified whilst the tbpba operon was represented by 28 unique alleles that could be assigned to seven classes. there were 1566 (34.8% variation) polymorphic nucleotide sites and 482 (32.1% variation) variable inferred amino acid positions among the 42 tbpba sequences. the tbpba operons of serotype a2 m. ...201020884693
mosaic structure and molecular evolution of the leukotoxin operon (lktcabd) in mannheimia (pasteurella) haemolytica, mannheimia glucosida, and pasteurella trehalosi.the mosaic structure and molecular evolution of the leukotoxin operon (lktcabd) was investigated by nucleotide sequence comparison of the lktc, lktb, and lktd genes in 23 mannheimia (pasteurella) haemolytica, 6 mannheimia glucosida, and 4 pasteurella trehalosi strains. sequence variation in the lkta gene has been described previously (r. l. davies et al., j. bacteriol. 183:1394-1404, 2001). the leukotoxin operon of m. haemolytica has a complex mosaic structure and has been derived by extensive i ...200211741868
evaluation of ewe vaccination as a tool for increasing bighorn lamb survival following pasteurellosis epizootics.we conducted field and laboratory experiments to evaluate whether treating pregnant bighorn ewes with a combination of an experimental pasteurella trehalosi and mannheimia haemolytica (formerly p. haemolytica) vaccine and a commercially-available bovine p. multocida and m. haemolytica vaccine would increase lamb survival following a pneumonia epidemic. three free-ranging bighorn herds affected by pasteurellosis outbreaks between november 1995 and june 1996 were included in the field experiment. ...200111272504
phylogenetic relationships and diversity within the pasteurella haemolytica complex based on 16s rrna sequence comparison and outer membrane protein and lipopolysaccharide analysis.the outer membrane protein (omp) and lipopolysaccharide (lps) profiles of 30 untypeable isolates of pasteurella haemolytica were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and compared with the profiles of typeable isolates. the phylogenetic relationships of 28 isolates representing each of the serotypes of p. haemolytica and pasteurella trehalosi, as well as untypeable isolates of p. haemolytica, were determined by comparing 16s rrna sequences. the analysis of the omp ...19968782683
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