an evaluation of the effect of various concentrations of the clemson university pasteurella multocida drinking water vaccine on the immune response against fowl cholera disease in turkey. 1977605013
acquired resistance in turkey poults to pasteurella multocida (p-1059 strain) during aflatoxin consumption.the potential for impairment by aflatoxin of the development of acquired immunity to fowl cholera in 8 week old turkey poults from a commercial flock was investigated by challenge of vaccinated poults at 10, 12, and 16 weeks of age. sixty poults were allotted to 4 groups: a) aflatoxin fed, nonvaccinated; b) aflatoxin fed, vaccinated; c) control fed, nonvaccinated; and d) control fed, vaccinated. aflatoxin b-1 (.5 ppm) was added to the control ration of groups a and b from 49 to 70 days of age. t ...1978724596
effect of furazolidone in feed on immune response of turkeys vaccinated with live pasteurella multocida in drinking 2 trials, turkey poults were fed diets containing 0.011% furazolidone: in one trial until 2, 3, 4, 5, 6, or 7 days before vaccination with live pasteurella multocida (cu) in the drinking water; and in a second trial with the furazolidone added to the diet 4 days before the vaccination or 1, 3 or 5 days after. this 0.011% level of medication did not appear to reduce the immune response.1976962760
transmission of pasteurella multocida on california turkey premises in 1988-89.restriction endonuclease analysis (rea) of whole-cell dna was used to determine possible sources of pasteurella multocida for each outbreak of fowl cholera occurring in turkey flocks in eight commercial poultry companies in california from october 1988 to september 1989. over this period, 179 isolates of p. multocida were obtained from dead turkeys in 80 meat and breeder flocks on 43 premises. p. multocida was isolated from wildlife on five premises. isolates were characterized by subspecies, se ...19921627098
serologic associations between fowl cholera and other part of a case-control study designed to identify fowl cholera risk factors, 2087 blood samples were collected from 71 california meat-turkey flocks. samples were tested for antibodies to three mycoplasmas and four viruses pathogenic for turkeys. flocks that had antibodies to newcastle disease virus and/or mycoplasma meleagridis had an increased risk of having an outbreak of fowl cholera. this information should prove useful for fowl cholera control programs in meat turkeys.19911786004
a survey of potential virulence markers from avian strains of pasteurella multocida.twenty-four isolates of pasteurella multocida from clinical cases of fowl cholera and the clemson university vaccine strain were surveyed for the presence of potential virulence markers. membrane proteins, enzymatic activity of the membrane proteins, and carbohydrate fermentation patterns were also determined to demonstrate phenotypic relationships within the groups. few differences were found in these phenotypic characteristics among the isolates. almost all the organisms produced siderophore a ...19912024443
serological surveillance for antibodies against different avian infectious agents on turkey flocks naturally infected with turkey rhinotracheitis.turkey rhinotracheitis (trt) in the state of baden-württemberg, germany appears to have become endemic affecting all farms and every new restocking. in 5 meat turkey flocks, serological surveillance for antibodies to trt-, ib- and ibd-viruses, as well as to pasteurella multocida were carried out using elisa tests. furthermore, sera were examined for the presence of antibodies to avian adenovirus (celo virus), reo- and paramyxo-viruses 1 and 3. the birds were bled at 4 week intervals starting at ...19902168612
a survey of infectious diseases in wild turkeys (meleagridis gallopavo silvestris) from arkansas.wild turkeys (meleagridis gallopavo silvestris) trapped as part of a relocation program by the arkansas game and fish commission were tested for selected infectious diseases and parasites. the 45 birds were trapped at four locations in pope, scott, and montgomery counties (arkansas, usa). forty-four blood samples for serology, 27 blood smears and 12 fecal samples were collected. of the serum samples tested, 20 of 44 (45%) were positive for pasteurella multocida by enzyme-linked immunosorbent ass ...19902250323
effects of bursectomy, irradiation, and cyclophosphamide on turkeys vaccinated with cu cholera strain.turkeys surgically bursectomized, irradiated, and/or injected with cyclophosphamide at 1 day were vaccinated with the live clemson university (cu) strain of pasteurella multocida. bursectomized turkeys vaccinated via drinking water or wing-web puncture at 7 weeks and challenged at 11 weeks had a significantly (p less than 0.05) lower survival rate after challenge than unbursectomized controls. bursectomized and unbursectomized turkeys vaccinated via drinking water at 7 weeks, revaccinated via th ...19873107538
rabbit pasteurellosis: induced disease and vaccination.pasteurellosis was induced in rabbits by conjunctival inoculation with 2 strains of pasteurella multocida. the ld50 of strain p1062 (a bovine isolate) was 10(5.1) colony-forming units and that of strain p1059 (a turkey isolate) was 10(5.5) colony-forming units. pasteurella-free rabbits were vaccinated iv or mucosally with boiled cells of p multocida or a cross-reactive uridine diphosphogalactose epimerase-deficient mutant of escherichia coli j5. in rabbits challenge exposed with p multocida stra ...19883282457
characterization of an avian cholera epizootic in wild birds in western nebraska.avian cholera killed an estimated 2500 birds in western nebraska and eastern wyoming from 28 november 1985 to late january 1986. wild mallards (anas platyrhynchos) suffered the most losses. other wild waterfowl, wild turkeys (meleagris gallopavo), a few domestic fowl, and a bald eagle (haliaeetus leucocephalus) also died. pasteurella multocida serotype 1 was the predominant isolate from these carcasses. cold, wet weather persisted throughout the outbreak, but daily losses in the flock of 50,000 ...19883382368
avian cholera in a gyrfalcon (falco rusticolus).a 2-year-old female gyrfalcon (falco rusticolus) was being used to hunt ducks in southeastern wyoming during an outbreak of avian cholera in waterfowl and wild turkeys. while out overnight, the falcon consumed a bird. within 24 hours the falcon was anorectic, and it was found dead approximately 48 hours following ingestion of wild prey. gross and microscopic lesions were typical of avian cholera, and pasteurella multocida serotype 1 was isolated from tissues of the falcon.19873619832
fowl cholera bacterins: host-specific cross-immunity induced in turkeys with pasteurella multocida propagated in embryonating turkey eggs. 19744133381
lysates of turkey-grown pasteurella multocida: protection against homologous and heterologous serotype challenge exposures.pastereulla multocida organisms were separated from the blood of experimentally infected turkeys by differential centrifugation. an average of 92% of the residual host-cell contamination was removed from the pasteurellas by density gradient centrifugation in sucrose. sucrose suspensions of the turkey-grown pasteurellas partially lysed after freezing and thawing. treatment of freeze-thawed suspensions with dnase, hyaluronidase, lysozyme, edta, and triton x-100 did not influence their ability to i ...19816803619
accidental penetration of hands with virulent and avirulent pasteurella multocida of turkey origin. 19807271639
characterisation of pasteurella multocida isolated from fowl cholera outbreaks on turkey farms.biochemical profiles, restriction endonuclease analysis (rea) and ribotyping were used to investigate pasteurella multocida isolates from outbreaks of fowl cholera on 7 turkey farms in new south wales. while only a single isolate was available from 5 of the farms, multiple isolates, 4 and 12 respectively, were available from the other 2 farms. the available field evidence suggested that 8 outbreaks had occurred with one farm suffering 2 outbreaks. the isolates obtained were all confirmed as past ...19957544114
expression of iron-regulated outer membrane proteins by porcine strains of pasteurella multocida.the outer membrane protein (omp) profiles of two strains of capsular type a pasteurella multocida isolated from the lungs of pigs with enzootic pneumonia were studied. sarkosyl extracted omps from p. multocida grown under iron-restricted and iron-replete conditions were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot analysis. results showed that the iron-regulated outer membrane proteins (iromps) with molecular masses of 74 kda, 94 kda, 99 kda and 109 kda ...19957704842
identification of common antigens of serotype 1 and serotype 3 pasteurella multocida in poultry expressed in vivo.eight groups of chicken immune sera and one group of turkey immune sera were used to identify unique in vivo-expressed antigens of pasteurella multocida by western immunoblotting. when antisera were prepared against the detergent-insoluble fraction of in vivo-grown p-1059, live x-73, live p-1059, live 86-1913, live p-1662, and live p-1702, they recognized four major antigens on immunoblots of the detergent-insoluble fraction of in vivo-grown serotype 1 and in vivo-grown serotype 3. these four an ...19947980285
detection of pasteurella multocida-specific dna in turkey flocks by use of the polymerase chain reaction.a polymerase chain reaction (pcr)-based assay using primers constructed to amplify the gene (psl) encoding the p6-like protein (psl) of pasteurella multocida was developed. after southern blotting and hybridization with psl, the assay (pcr-h) was found to be specific (it did not detect a variety of other avian bacterial pathogens) and sensitive (detected > or = 10 p. multocida organisms or > or = 24 femtograms of extracted p. multocida dna). samples were collected from the oropharynx of randomly ...19979356715
ultrastructural location of the cross-protection factor on in vivo and in vitro grown pasteurella multocida.pasteurella multocida from infected turkey tissues expresses a unique immunogen called cross-protection factor (cpf) that induces immunity to challenge by both homologous and heterologous serotypes. in this study, we used a monoclonal antibody (amp mab) to cpf and protein a-colloidal gold (pacg) to locate cpf on p. multocida. after incubation with amp mab and pacg, cpf was detected at the bacterial surface and cell periphery of p. multocida in infected turkey liver and p. multocida isolated from ...200111785898
occurrence of conjunctivitis, sinusitis and upper region tracheitis in japanese quail (coturnix coturnix japonica), possibly caused by mycoplasma gallisepticum accompanied by cryptosporidium sp. infection.on a farm raising approximately 75,000 japanese quail (coturnix coturnix japonica) for egg production, the diseased quail showed clinical signs of swelling of the head, nasal discharge, increased lacrimation, and decreased egg production. the flock experienced a mortality rate of 5.7% per day. macroscopic observation revealed large, gelatinous masses of caseous exudate in the sinuses, egg peritonitis, and airsacculitis. microscopically, non-purulent or purulent inflammation accompanied by lympho ...200212396337
molecular epidemiology investigation of outbreaks of fowl cholera in geographically related poultry flocks.the present work describes two cases of consecutive fowl cholera outbreak series occurring in goose and turkey flocks. most strains isolated from epidemiologically related outbreaks showed genetic relatedness, as revealed by enterobacterial repetitive intergenic consensus-pcr and pulsed-field gel electrophoresis, suggesting that the consecutive outbreaks were due to recurrences rather than reinfections.200515956431
characterization of avian strains of pasteurella multocida by restriction endonuclease and amplified fragment length polymorphism.avian strains of pasteurella multocida were typed by employing restriction endonuclease analysis (rea) and single enzyme-amplified fragment length polymorphism (aflp) to evaluate their applicability for epidemiological studies of fowl cholera outbreaks. a total of 72 strains isolated from different avian species (chicken, duck, turkey, quail and goose) belonging to various geographical regions of india were characterized. rea using two different enzymes hhai and hpaii produced 9 and 18 clusters ...200616427104
full genome sequence and some biological properties of reticuloendotheliosis virus strain apc-566 isolated from endangered attwater's prairie chickens.reticuloendotheliosis virus (rev) causes runting, high mortality, immunosuppression, and chronic neoplasia associated with t and/or b cell lymphomas in a variety of domestic and wild birds, including attwater's prairie chickens (apc) (tympanuchus cupido attwateri). the complete proviral sequence of a recent rev isolate from apc (rev apc-566) was determined. this virus was isolated from an apc maintained in captivity in a reproduction program intended to avoid its extinction. rev apc-566 was dete ...200717098316
quantitative resistance level (mic) of bacterial pathogens (escherichia coli, pasteurella multocida, pseudomonas aeruginosa, salmonella sp., staphylococcus aureus) isolated from chickens and turkeys: national resistance monitoring by the bvl 2004/ the study 2004/2005, the current quantitative resistance level of escherichia coli, pasteurella multocida, pseudomonas aeruginosa, salmonella sp. and staphylococcus aureus from chickens and turkeys was determined for the first time within the framework of the national resistance monitoring of the federal office of consumer protection and food safety (bvl). the objective was to implement a valid database on the basis of which the development and spread of resistance can be evaluated and monito ...200717939460
sialic acid uptake is necessary for virulence of pasteurella multocida in turkeys.many pathogenic bacteria employ systems to incorporate sialic acid into their membranes as a means of protection against host defense mechanisms. in pasteurella multocida, an opportunistic pathogen which causes diseases of economic importance in a wide range of animal species, sialic acid uptake plays a role in a mouse model of systemic pasteurellosis. to further investigate the importance of sialic acid uptake in pathogenesis, sialic acid uptake mutants of an avian strain of p. multocida p-1059 ...200919366625
mortality and disease in wild turkeys ( meleagris gallopavo silvestris) in ontario, canada, from 1992 to 2014: a retrospective review.wild turkeys ( meleagris gallopavo silvestris) were extirpated from ontario, canada, in the early 1900s due to unregulated over-hunting and habitat loss. despite a successful reintroduction program and strong population numbers, information regarding the health of wild turkeys in ontario is scarce. a 22-yr (1992-2014) retrospective study was performed to evaluate diagnostic data, including the cause(s) and contributors to death, in wild turkeys submitted to the ontario-nunavut node of the canadi ...201627610724
pasteurella multocida in wild mammals and birds in california: prevalence and virulence for turkeys.samples collected from the oropharynx of wild mammals and birds trapped on 36 turkey farms in california were evaluated for the presence of pasteurella multocida. a total of 966 animals were collected from 18 premises that had experienced an outbreak of fowl cholera within the past 2-8 months; samples were collected from 16 of these 18 premises within 2-8 weeks of outbreak notification and while the infected flock was still present. a total of 939 animals were trapped from an additional 18 premi ...19883382384
differentiating turkey postvaccination isolants of pasteurella multocida using arbitrarily primed polymerase chain reaction.the chromosomal dna of 29 field isolants of pasteurella multocida from commercial turkey farms in missouri and the avirulent clemson university (cu) and m-9 vaccine strains of p. multocida were tested using the arbitrarily primed polymerase chain reaction (ap-pcr) in combination with 32p-labeled deoxycytidine triphosphate (dctp) and high-resolution gel electrophoresis. the 29 field isolants of p. multocida were isolated from outbreaks of fowl cholera in turkey flocks in which vaccination with th ...19989645317
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