Publications

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[production of pyoverdin by pseudomonas fluorescens in human blood at 4 degrees c: association with proteins and the cytotoxic effect of the pigment].pyoverdin was purified from pseudomonas fluorescens cultured in a synthetic medium. cytotoxic effect on human leukocytes was assayed. death and lysis was observed depending on concentration and time. sub-lytic dose decreased leukocyte phagocytosis. the pigment was produced by p. fluorescens in blood stored at 4 degrees c. saline precipitation of plasma showed that globulin and albumin fraction retained 46% and 37% of pyoverdin, respectively. by means of dialysis it was possible to determine that ...19902125740
[evaluation of culture media used in the differentiation of pseudomonas aeruginosa, pseudomonas putida, and pseudomonas fluorescens, isolated from clinical human material]. 19744549846
pollution of modern metalworking fluids containing biocides by pathogenic bacteria in france. reexamination of chemical treatments accuracy.pollution by pathogenic bacteria was examined in 150 french metalworking fluid samples. gram-negative micro-organisms such as salmonella spp., shigella spp., and vibrio spp. as well as gram-positive cocci were never isolated. nevertheless opportunistic pathogens such as pseudomonas aeruginosa and klebsiella pneumoniae still contaminated these fluids with an isolation frequency of 17% of samples for each. these two micro-organisms failed to grow or even survive in vitro in sterile cutting fluids ...19957489767
determination of gamma-aminobutyric acid levels in human cerebrospinal fluid using pseudomonas.the principle objective was to demonstrate the efficacy of a bacterial, radioligand, competitive binding method in determining gamma-aminobutyric acid (gaba) levels in human cerebrospinal fluid (csf). in a double blind study, csf gaba concentrations were measured by the bacterial method using a mutant of pseudomonas fluorescens and by a standard radioligand competitive binding assay using rat brain membranes. linear regression analysis demonstrated a highly significant correlation (r = 0.84; p < ...19957762793
[pyoverdin from pseudomonas fluorescens: binding to the globulin fraction of serum and its effect on serological reaction titers].pseudomonas fluorescens grows and produces pigment in refrigerated human blood at 4 degrees c. saline precipitation of plasma showed that globulin and albumin fractions retained pyoverdin at different concentrations. by dialysis it was possible to determine that the pigment attached or aggregated to the protein in total plasma as well as in the fraction obtained by saline precipitation. a greater binding was observed at the globulin fraction. gamma-globulin immunological activity was reduced due ...19947973183
an efficient conversion of (3r,3'r,6'r)-lutein to (3r,3's,6'r)-lutein (3'-epilutein) and (3r,3'r)-zeaxanthin.two dietary carotenoids, (3r,3'r,6'r)-lutein (1) and (3r,3'r)-zeaxanthin (2), and their metabolite (3r,3's,6'r)-lutein (3'-epilutein) (3) accumulate in human serum, milk, and ocular tissues. there is increasing evidence that compounds 1 and 2 play an important role in the prevention of age-related macular degeneration. therefore, the availability of these carotenoids for metabolic studies and clinical trials is essential. compound 1 is isolated from extracts of marigold flowers (tagete erecta) a ...200312542348
survivability and long-term preservation of bacteria in water and in phosphate-buffered saline.to evaluate the suitability of using sterile water and phosphate-buffered saline (pbs) for preservation of bacteria pathogenic to plants or humans.200312803555
the use of broth culture filtrates of pseudomonas fluorescens for the destruction of non-specific thermostabile (at 56 degrees c) influenza virus inhibitors it human and animal sera. 195813594717
effects of pseudomonas fluorescens m3/6 bacterial protease on plasmin system and plasminogen activation.heat-stable proteases produced by the psychrotroph pseudomonas fluorescens m3/6 have been shown to affect the plasmin system in milk, which in turn will affect the quality of processed milk. the m3/6 proteases cause dissociation of plasmin from casein in minimally processed milk. the objective of this work was to study the effect of m3/6 protease on the plasmin system, as well as its role in plasminogen activation, under commonly applied cheese-making conditions. isolated m3/6 protease was added ...200516162512
crystal structure of homo sapiens kynureninase.kynureninase is a member of a large family of catalytically diverse but structurally homologous pyridoxal 5'-phosphate (plp) dependent enzymes known as the aspartate aminotransferase superfamily or alpha-family. the homo sapiens and other eukaryotic constitutive kynureninases preferentially catalyze the hydrolytic cleavage of 3-hydroxy-l-kynurenine to produce 3-hydroxyanthranilate and l-alanine, while l-kynurenine is the substrate of many prokaryotic inducible kynureninases. the human enzyme was ...200717300176
pseudomonas fluorescens sbw25 biofilm and planktonic cells have differentiable raman spectral profiles.biofilms, and other bacterial aggregations, are of significance in both environmental microbiology and in plant and human pathogenesis. comparative single-cell raman spectral analysis can differentiate between planktonic bacteria and those recovered from biofilms and appears to offer a new means by which to investigate bacterial cell physiology, metabolic status, and stress under different environmental conditions.200717345138
structure-function relationships in a bacterial ding protein.a recombinant ding protein from pseudomonas fluorescens has been previously shown to have a phosphate-binding site, and to be mitogenic for human cells. here we report the three-dimensional structure of the protein, confirming a close similarity to the "venus flytrap" structure seen in other human and bacterial phosphate-binding proteins. site-directed mutagenesis confirms the role of a key residue involved in phosphate binding, and that the mitogenic activity is not dependent on this property. ...200717612529
crystallization, diffraction data collection and preliminary crystallographic analysis of ding protein from pseudomonas fluorescens.pfluding is a phosphate-binding protein expressed in pseudomonas fluorescens. this protein is clearly distinct from the bacterial abc transporter soluble phosphate-binding protein psts and is more homologous to eukaryotic ding proteins. interestingly, bacterial ding proteins have only been detected in certain pseudomonas species. although ding proteins seem to be ubiquitous in eukaryotes, they are systematically absent from eukaryotic genomic databases and thus are still quite mysterious and poo ...200717620718
lysophospholipase from the human blood fluke, schistosoma japonicum.given the unusual nature of the schistosome surface (a highly unusual lipid bi-layer) and the central role of the schistosome tegument in host-parasite relations, an enhanced understanding of the lipid biochemistry of the schistosome surface can be expected to provide new insights into schistosome pathogenesis and lead to new interventions.200817709268
biochemical characterization of a flavin adenine dinucleotide-dependent monooxygenase, ornithine hydroxylase from pseudomonas aeruginosa, suggests a novel reaction mechanism.pyoverdin is the hydroxamate siderophore produced by the opportunistic pathogen pseudomonas aeruginosa under the iron-limiting conditions of the human host. this siderophore includes derivatives of ornithine in the peptide backbone that serve as iron chelators. pvda is the ornithine hydroxylase, which performs the first enzymatic step in preparation of these derivatives. pvda requires both flavin adenine dinucleotide (fad) and nicotinamide adenine dinucleotide phosphate (nadph) for activity; it ...200717900176
effect of bacterial antagonists on lettuce: active biocontrol of rhizoctonia solani and negligible, short-term effects on nontarget microorganisms.the aim of this study was to assess the biocontrol efficacy against rhizoctonia solani of three bacterial antagonists introduced into naturally rhizoctonia-infested lettuce fields and to analyse their impact on the indigenous plant-associated bacteria and fungi. lettuce seedlings were inoculated with bacterial suspensions of two endophytic strains, serratia plymuthica 3re4-18 and pseudomonas trivialis 3re2-7, and with the rhizobacterium pseudomonas fluorescens l13-6-12 7 days before and 5 days a ...200818248441
isolation and identification of rhizoxin analogs from pseudomonas fluorescens pf-5 by using a genomic mining strategy.the products synthesized from a hybrid polyketide synthase/nonribosomal peptide synthetase gene cluster in the genome of pseudomonas fluorescens pf-5 were identified using a genomics-guided strategy involving insertional mutagenesis and subsequent metabolite profiling. five analogs of rhizoxin, a 16-member macrolide with antifungal, phytotoxic, and antitumor activities, were produced by pf-5, but not by a mutant with an insertion in the gene cluster. the five rhizoxin analogs, one of which had n ...200818344330
involvement of a phospholipase c in the hemolytic activity of a clinical strain of pseudomonas fluorescens.pseudomonas fluorescens is a ubiquitous gram-negative bacterium frequently encountered in hospitals as a contaminant of injectable material and surfaces. this psychrotrophic bacterium, commonly described as unable to grow at temperatures above 32 degrees c, is now considered non pathogenic. we studied a recently identified clinical strain of p. fluorescens biovar i, mfn1032, which is considered to cause human lung infection and can grow at 37 degrees c in laboratory conditions.200818973676
polyol-specific long-chain dehydrogenases/reductases of mannitol metabolism in aspergillus fumigatus: biochemical characterization and ph studies of mannitol 2-dehydrogenase and mannitol-1-phosphate 5-dehydrogenase.functional genomics data suggests that the metabolism of mannitol in the human pathogen aspergillus fumigatus involves the action of two polyol-specific long-chain dehydrogenases/reductases, mannitol-1-phosphate 5-dehydrogenase (m1pdh) and mannitol 2-dehydrogenase (m2dh). the gene encoding the putative m2dh was expressed in escherichia coli, and the purified recombinant protein was characterized biochemically. the predicted enzymatic function of a nad(+)-dependent m2dh was confirmed. the enzyme ...200918983992
functional recognition imaging using artificial neural networks: applications to rapid cellular identification via broadband electromechanical response.functional recognition imaging in scanning probe microscopy (spm) using artificial neural network identification is demonstrated. this approach utilizes statistical analysis of complex spm responses at a single spatial location to identify the target behavior, which is reminiscent of associative thinking in the human brain, obviating the need for analytical models. we demonstrate, as an example of recognition imaging, rapid identification of cellular organisms using the difference in electromech ...200919752493
immunoproteomic identification of polyvalent vaccine candidates from vibrio parahaemolyticus outer membrane proteins.bacterium is still a major cause of many infectious diseases and a global threat to human health, aquaculture, and animal feeding. prevention by vaccination is the most efficient and economical way of fighting bacterial diseases, but one of the persistent challenges to prevent bacterial infections and disease transmissions is the existence of multiple bacterial species, families, and genera and the lack of efficient polyvalent vaccines against them. the information on candidate immunogens for po ...201020148598
evolution of new enzymatic function by structural modulation of cysteine reactivity in pseudomonas fluorescens isocyanide hydratase.isocyanide (formerly isonitrile) hydratase (ec 4.2.1.103) is an enzyme of the dj-1 superfamily that hydrates isocyanides to yield the corresponding n-formamide. in order to understand the structural basis for isocyanide hydratase (ich) catalysis, we determined the crystal structures of wild-type and several site-directed mutants of pseudomonas fluorescens ich at resolutions ranging from 1.0 to 1.9 å. we also developed a simple uv-visible spectrophotometric assay for ich activity using 2-naphthyl ...201020630867
isolation of culturable microorganisms from free-ranging bottlenose dolphins (tursiops truncatus) from the southeastern united states.reports of diseases in marine mammals are increasing worldwide, however our understanding of the microorganisms associated with marine mammals is still limited. in this study, we cultured bacteria and fungi isolated from the upper respiratory tract (blowhole), gastric fluid and anus of 180 wild bottlenose dolphins (tursiops truncatus) from two estuarine locations along the southeastern atlantic coast of the united states. a total of 339 and 491 isolates from charleston, sc (chs) and indian river ...201020888150
structure, mechanism, and substrate specificity of kynureninase.the kynurenine pathway is the major route for tryptophan catabolism in animals and some fungi and bacteria. the procaryotic enzyme preferentially reacts with l-kynurenine, while eucaryotic kynureninases exhibit higher activity with 3-hydroxy-l-kynurenine. crystallography of kynureninases from pseudomonas fluorescens (pfkyn) and homo sapiens (hskyn) shows that the active sites are nearly identical, except that his-102, asn-333, and ser-332 in hskyn are replaced by trp-64, thr-282, and gly-281 in ...201021167323
soluble periplasmic production of human granulocyte colony-stimulating factor (g-csf) in pseudomonas fluorescens.cost-effective production of soluble recombinant protein in a bacterial system remains problematic with respect to expression levels and quality of the expressed target protein. these constraints have particular meaning today as "biosimilar" versions of innovator protein drugs are entering the clinic and the marketplace. a high throughput, parallel processing approach to expression strain engineering was used to evaluate soluble expression of human granulocyte colony-stimulating factor (g-csf) i ...201121396452
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