| no correlation of baba2 with vaca and caga genotypes of helicobacter pylori and grading of gastritis from peptic ulcer disease patients in brazil. | the baba2 gene, which encodes a blood-group antigen-binding adhesin that mediates attachment of helicobacter pylori to human lewis(b) antigens on gastric epithelial cells, has been associated with a higher risk of peptic ulcer and gastric cancer. the purpose of this study was to ascertain the frequency of baba2 genotype in h. pylori strains of patients with peptic ulcer and to correlate with other virulence factors. | 2005 | 16302986 |
| role of surfactant protein d (sp-d) in innate immunity in the gastric mucosa: evidence of interaction with helicobacter pylori lipopolysaccharide. | surfactant protein d (sp-d) is a collagenous glycoprotein, a collectin, which functions as a pathogen-associated molecular pattern (pamp) recognition receptor in the innate immune response. although originally identified in the lung as a component of surfactant, sp-d also occurs in the gastric mucosa at the luminal surface and within gastric pits of mucus-secreting cells. infection with the gastroduodenal pathogen helicobacter pylori up-regulates expression of sp-d in human patients with gastrit ... | 2005 | 16303091 |
| helicobacter felis and helicobacter bizzozeronii induce gastric parietal cell loss in mongolian gerbils. | non-pylori helicobacter infections are associated with gastritis, gastric ulcers and malt lymphomas in man. approximately 50% of these are caused by helicobacters commonly found in dogs and cats, including helicobacter felis, helicobacter bizzozeronii and h. salomonis. in contrast to helicobacter pylori, the virulence mechanisms of these species are unknown. in this study the virulence of h. felis, h. bizzozeronii and h. salomonis was investigated in mongolian gerbils. female spf gerbils were in ... | 2006 | 16311055 |
| helicobacter pylori but not high salt induces gastric intraepithelial neoplasia in b6129 mice. | helicobacter pylori is responsible for most human stomach cancers. gastric cancer also is overrepresented in populations consuming high-salt diets. attempts to test the hypothesis that high salt promotes h. pylori carcinogenesis have been hindered by the lack of a wild-type mouse model. based on pilot observations of unexpectedly early gastric adenocarcinoma in c57bl/6 x 129s6/svev (b6129) mice infected with helicobacter felis, we conducted a study to characterize h. pylori infection in these mi ... | 2005 | 16322215 |
| interleukin-8, cyclo-oxygenase-2, and trefoil factor family 1 gene expression and their association with helicobacter pylori infection in the remnant stomach. | the risk factors for secondary stomach carcinogenesis after distal gastrectomy have not been evaluated in detail. | 2005 | 16341482 |
| association between herpes simplex virus type 1 and helicobacter pylori in us adolescents. | to understand how helicobacter pylori infection is acquired and the role that herpes simplex virus type 1 (hsv-1) may have, we determined whether an association between hsv and h pylori exists at the individual level and for what reason. | 2006 | 16360609 |
| [relationship between helicobacter pylori caga-shp-2 interaction and gastric cancer]. | | 2005 | 16363503 |
| [immune and inflammatory reaction to helicobacter pylori in the stomach]. | | 2005 | 16363522 |
| [transmission route of h. pylori]. | | 2005 | 16363526 |
| [long-term follow-up of h. pylori infection--from gastritis to gastric cancer]. | | 2005 | 16363529 |
| [recent topics on important drugs for h. pylori eradication: omeprazole]. | | 2005 | 16363556 |
| [concomitant dosing of a h2 receptor antagonist (h2ra) with a triple therapy increases the cure rate of helicobacter pylori infection]. | | 2005 | 16363566 |
| [long-term outcome in patients with gastric malt lymphoma after h. pylori eradication]. | | 2005 | 16363592 |
| [influence of cytochrome p450 2c19 genetic polymorphism on h. pylori eradication therapy]. | | 2005 | 16363603 |
| the cell-specific phenotype of the polymorphic vaca midregion is independent of the appearance of the cell surface receptor protein tyrosine phosphatase beta. | there are two alleles, m1 and m2, of the midregion of the vacuolating cytotoxin gene (vaca) of helicobacter pylori which code for toxins with different cell specificities. here we describe the construction of five chimeric strains in which regions of vaca were exchanged between the two genotypes. by analyzing the toxicity of these strains for hela and rk13 cells we have confirmed that a 148-amino-acid region determines the phenotypic differences between the two forms of the protein and that this ... | 2006 | 16368956 |
| bovine lactoferrin is effective to suppress helicobacter pylori colonization in the human stomach: a randomized, double-blind, placebo-controlled study. | bovine lactoferrin (blf) has antibacterial activity against helicobacter pylori in vitro and is effective to suppress bacterial colonization in mice. the aim of our study was to evaluate the efficacy of orally administered blf on h. pylori colonization in humans by a randomized, double-blind, placebo-controlled study. fifty-nine healthy subjects positive for h. pylori infection were recruited. subjects were randomized into two groups. the blf group received blf tablets at a dosage of 200 mg b.i. ... | 2005 | 16369731 |
| the epidemiology of non-hodgkin lymphoma. | non-hodgkin lymphoma (nhl) includes a group of more than 20 different malignant lymphoproliferative diseases that originate from lymphocytes. rates of nhl have increased dramatically over the past few decades, although the rate of increase has recently slowed. it is now the sixth most common cancer in australia. globally, it is somewhat more common in men than in women, and rates are highest in north america and australia. the causes of the increase in nhl rates are largely unknown. the best des ... | 2005 | 16373224 |
| preferential expression of cyclooxygenase-2 in colonic-phenotype of gastric intestinal metaplasia: association with helicobacter pylori and gastric carcinoma. | gastric intestinal metaplasia (gim) associated with h. pylori (hp) has been considered a premalignant lesion. however, gim phenotype associated with hp infection and gastric cancer is unclear. the expression of cox-2 in relation to gim phenotype is also unknown. | 2006 | 16394872 |
| the global health burden of infection-associated cancers in the year 2002. | several infectious agents are considered to be causes of cancer in humans. the fraction of the different types of cancer, and of all cancers worldwide and in different regions, has been estimated using several methods; primarily by reviewing the evidence for the strength of the association (relative risk) and the prevalence of infection in different world areas. the estimated total of infection-attributable cancer in the year 2002 is 1.9 million cases, or 17.8% of the global cancer burden. the p ... | 2006 | 16404738 |
| epstein-barr virus (ebv) infection and p53 protein expression in gastric carcinoma. | in the presented studies p53 protein expression was evaluated in samples of gastric carcinoma originating from 32 selected adult patients (with documented diagnosis of adenocarcinoma of the stomach and without the presence of helicobacter pylori infection). among the patients 14 individuals carried ebv-positive gastric carcinoma (group 1) while the 18 remaining patients carried ebv-negative gastric carcinoma (group 2). ebv infection was detected testing the tissue material for the presence of eb ... | 2006 | 16413625 |
| [cytochrome p450 2c19 genotype -based second line therapy with amoxicillin and lansoprazole after failure of initial helicobacter pylori eradication therapy]. | | 2005 | 16416832 |
| helicobacter pylori has stimulatory effects on naive t cells. | despite an apparently active host response, helicobacter pylori infection can persist for life. unexpectedly, t cells from apparently uninfected individuals respond to h. pylori antigen by proliferating. also, the t-cell proliferative response appears to be less in infected compared with uninfected individuals. | 2006 | 16423086 |
| herpes simplex virus type 1 in peptic ulcer disease: an inverse association with helicobacter pylori. | to assess the frequency of herpes simplex virus type i in upper gastrointestinal tract ulcers and normal mucosa with the modern and better assays and also with a larger number of well characterized patients and controls and its relationship to helicobacter pylori(h pylori). | 2005 | 16425358 |
| urokinase system expression in gastric carcinoma: prognostic impact in an independent patient series and first evidence of predictive value in preoperative biopsy and intestinal metaplasia specimens. | the prognostic relevance of urokinase-type plasminogen activator (u-pa), u-pa receptor (u-par), and plasminogen activator inhibitor 1 (pai-1) in gastric carcinoma was demonstrated in an independent patient series. to the authors' knowledge,the roles of these activators as predictors of aggressive phenotypes in preoperative biopsies, helicobacter pylori infection, and intestinal metaplasia have to date not been investigated simultaneously in resected tumors. the objectives of the current study we ... | 2006 | 16435385 |
| nickel represses the synthesis of the nickel permease nixa of helicobacter pylori. | nickel acquisition is necessary for urease activity, a major virulence factor of the human gastric pathogen helicobacter pylori. nixa was identified as a specific nickel uptake system in this organism. addition of small amounts of nickel to media strongly stimulates urea hydrolysis. on the other hand, high nickel concentrations are deleterious to cell growth. as a possible protective reaction, nickel uptake seems to be reduced in h. pylori grown in nickel-rich media. these observations led to in ... | 2006 | 16452405 |
| carbonic anhydrase inhibitors: cloning and sulfonamide inhibition studies of a carboxyterminal truncated alpha-carbonic anhydrase from helicobacter pylori. | a library of sulfonamides/sulfamates has been investigated for the inhibition of the carboxyterminal truncated form of the alpha-carbonic anhydrase (ca, ec 4.2.1.1) isolated from the gastric pathogen helicobacter pylori (hpca). this enzyme, incorporating 202 amino acid residues, showed a catalytic activity similar to that of the full length hpca, with k(cat) of 2.35 x 10(5)s(-1) and k(cat)/k(m) of 1.56 x 10(7)m(-1)s(-1) at 25 degrees c and ph of 8.9, for the co(2) hydration reaction. all types o ... | 2006 | 16459077 |
| secretory leukocyte protease inhibitor expression in various types of gastritis: a specific role of helicobacter pylori infection. | secretory leukocyte protease inhibitor (slpi) represents a multifunctional protein of the gastrointestinal mucosa exerting antimicrobial and anti-inflammatory effects. slpi expression is generally induced during inflammation; however, helicobacter pylori-mediated gastritis is associated with significantly decreased antral slpi levels. the aim of the study was to investigate whether slpi downregulation of gastric mucosa represents a specific phenomenon of h. pylori infection or is generally linke ... | 2006 | 16462541 |
| helicobacter pylori regulates the expression of inhibitors of dna binding (id) proteins by gastric epithelial cells. | id transcription factors control proliferation, differentiation and apoptosis by inhibiting the dna binding of basic helix-loop-helix transcription factors. increased expression of id proteins promotes proliferation, inhibits differentiation, and is associated with intestinal tumorigenesis. we aimed to determine how helicobacter pylori may alter the expression of id proteins by gastric epithelial cells: it was hypothesised that h. pylori, a known carcinogen, would result in increased expression ... | 2006 | 16473539 |
| mannan-binding lectin in children with chronic gastritis. | the involvement of mannan-binding lectin (mbl) insufficiency in the pathogenesis of chronic gastritis (cg) in children was investigated. blood samples were collected from 78 paediatric patients suffering from cg associated with helicobacter pylori infection (group hp(+)) and from 41 with the disease not associated with such an infection (group hp(-)). control group consisted of 77 children. the frequency of mbl-2 gene mutations and serum protein concentrations did not differ significantly in bot ... | 2006 | 16476012 |
| expression of b7-h1 on gastric epithelial cells: its potential role in regulating t cells during helicobacter pylori infection. | helicobacter pylori infection is associated with gastritis, ulcers, and gastric cancer. the infection becomes chronic as the host response is unable to clear it. gastric epithelial cells (gec) play an important role during the host response, and their expression of class ii mhc and costimulatory molecules such as cd80 and cd86 suggests their role in local ag presentation. although t cells are recruited to the infected gastric mucosa, they have been reported to be hyporesponsive. in this study, w ... | 2006 | 16493058 |
| signalling pathways and molecular interactions of nod1 and nod2. | the nod (nucleotide-binding oligomerization domain) proteins nod1 and nod2 have important roles in innate immunity as sensors of microbial components derived from bacterial peptidoglycan. the importance of these molecules is underscored by the fact that mutations in the gene that encodes nod2 occur in a subpopulation of patients with crohn's disease, and nod1 has also been shown to participate in host defence against infection with helicobacter pylori. here, we focus on the molecular interaction ... | 2006 | 16493424 |
| the pak1 autoregulatory domain is required for interaction with nik in helicobacter pylori-induced nf-kappab activation. | helicobacter pylori, the etiological agent of various human gastric diseases, induces the transcription factor nuclear factor kappab (nf-kappab) and proinflammatory cytokines/chemokines. we have characterised the direct interaction between p21-activated kinase 1 (pak1) and nf-kappab-inducing kinase (nik) in h. pylori-infected epithelial cells. the dimerisation (di) motif, which is part of the nh2-terminal autoregulatory domain of pak1, is critical for this interaction, whereas nik forms complexe ... | 2006 | 16497167 |
| hla-dqb1 locus and gastric cancer in helicobacter pylori infection. | it has been suggested that the incidence of digestive diseases associated with helicobacter pylori is influenced by the strain diversity of h. pylori, factors involving the host or environment, and the duration of infection. the authors have previously reported that human leukocyte antigen (hla)-dqb1*0401 plays an important role in the development of atrophic gastritis in h. pylori infected patients. the aim of the present study was to investigate the relationship between hla-dqb1 genotype and c ... | 2006 | 16509868 |
| effects of helicobacter pylori infection on mucin expression in gastric carcinoma and pericancerous tissues. | helicobacter pylori infection is one of the major causes of human gastric carcinoma and can disturb the gastric mucosa barrier. mucins have not only lubricating and protecting functions, but are also related to signal transduction, turnover of gastric epithelium and carcinogenesis of gastric mucosa. the aim of this study was to investigate the relationship between h. pylori infection and aberrant mucin expression in patients with gastric carcinoma. | 2006 | 16509869 |
| nucleotide-binding oligomerization domain-1 and epidermal growth factor receptor: critical regulators of beta-defensins during helicobacter pylori infection. | host-pathogen interactions that allow helicobacter pylori to survive and persist in the stomach of susceptible individuals remain unclear. human beta-defensins (hbds), epithelial-derived antimicrobial peptides are critical components of host-defense at mucosal surfaces. the role of h. pylori-mediated nf-kappab and epidermal growth factor receptor (egfr) activation on beta-defensin expression was investigated. transient transfection studies utilizing beta-defensin promoter constructs were conduct ... | 2006 | 16513653 |
| [development and clinical application of invader assay--detection of resistant mutation to clarithromycin in helicobacter pylori]. | invader assay is a novel technology used for the direct analysis of genomic dna without pcr amplification, including the detection of single nucleotide polymorphisms (snps) in human genome. it has high accuracy and reproducibility. we have previously developed snps assays of cytochrome p450 (cyp) 2c19 and many other drug metabolized enzymes by invader technology to realize personalized medicine. here, we developed invader assays to detect mutations with resistance to clarithromycin (cam) in h. p ... | 2006 | 16548239 |
| theodore e. woodward award: global warming and the human stomach: microecology follows macroecology. | just as there have been 20th century changes in our "macroecology," including global warming, there have been alterations in our "microecology," involving the microbial populations that colonize the human body. helicobacterpylori, an ancient inhabitant of the human stomach, has been disappearing over the course of the 20th century. as such, by comparing h. pylori+ and h. pylori- persons, the consequences of its colonization can be determined. the presence of h. pylori is associated with increase ... | 2005 | 16555606 |
| bacteria and cancer: cause, coincidence or cure? a review. | research has found that certain bacteria are associated with human cancers. their role, however, is still unclear. convincing evidence links some species to carcinogenesis while others appear promising in the diagnosis, prevention or treatment of cancers. the complex relationship between bacteria and humans is demonstrated by helicobacter pylori and salmonella typhi infections. research has shown that h. pylori can cause gastric cancer or malt lymphoma in some individuals. in contrast, exposure ... | 2006 | 16566840 |
| cytotoxic 5-aryl-1-(4-nitrophenyl)-3-oxo-1,4-pentadienes mounted on alicyclic scaffolds. | the 5-aryl-1-(4-nitrophenyl)-3-oxo-1,4-pentadienyl pharmacophore was incorporated into four series of compounds 1-4. compounds 1a-g comprised a cluster of 3-arylidene-1-(4-nitrophenylmethylene)-2-oxo-3,4-dihydro-1h-naphthalenes while the analogues 2a-g consisted of a group of 6-arylidene-2-(4-nitrophenylmethylene)cyclohexanones. three other compounds prepared in this study were 1-(4-nitrophenylmethylene)-3-(3,4,5-trimethoxyphenylmethylene)-2-oxo-2,3-dihydro-1h-indene 3a as well as two 5-aryliden ... | 2006 | 16581158 |
| environment as a critical factor for the pathogenesis and outcome of gastrointestinal disease: experimental and human inflammatory bowel disease and helicobacter-induced gastritis. | environmental factors play an important role in the manifestation, course, and prognosis of diseases of the gastrointestinal tract such as inflammatory bowel disease (ibd) and helicobacter pylori-induced gastritis. these two disease complexes were chosen for a discussion of the contribution of environmental factors to the disease outcome in humans and animal models. dissecting complex diseases like ibd and helicobacter-induced gastritis has shown that the outcome of disease depends on the alleli ... | 2005 | 16582581 |
| correlation of epstein-barr virus and its encoded proteins with helicobacter pylori and expression of c-met and c-myc in gastric carcinoma. | to investigate the interrelationship of epstein-barr virus (ebv) and ebv- encoded proteins with helicobacter pylori (h pylori) infection and the expression of c-met and c-myc oncogene proteins in gastric carcinoma, and to explore their role in gastric carcinogenesis. | 2006 | 16609989 |
| role of toll-like receptors in health and diseases of gastrointestinal tract. | the human gastrointestinal (gi) tract is colonized by non-pathogenic commensal microflora and frequently exposed to many pathogenic organisms. for the maintenance of gi homeostasis, the host must discriminate between pathogenic and non-pathogenic organisms and initiate effective and appropriate immune and inflammatory responses. mammalian toll-like receptors (tlrs) are members of the pattern-recognition receptor (prr) family that plays a central role in the initiation of innate cellular immune r ... | 2006 | 16610014 |
| disposition of exogenous urea and effects of diet in rats. | although breath test using 13c-labeled urea (cas 57-13-6, ubt) is becoming popular for the diagnosis of helicobacter pylori (h. pylori) infection, disposition of exogenously given urea is not fully understood. the purpose of the present study is to elucidate the disposition of exogenous urea and to consider its relation with the ubt safety and biobehavior of endogenous urea. with 14c-labeled urea ([14c]urea), the absorption, distribution, metabolism and excretion including that into breathed air ... | 2006 | 16618019 |
| [expression and immunogenicity analysis of a recombinant fusion protein of v. cholera ctb and h. pylori ure i]. | to express fusion protein of the cholera toxin b subunit (ctb) and the urea membrane channel gene (ure i) of h. pylori in e. coli, and analyze its immunogenicity. | 2006 | 16643777 |
| the infection connection. helicobacter pylori is more than just the cause of gastric ulcers--it offers an unprecedented opportunity to study changes in human microecology and the nature of chronic disease. | | 2006 | 16670677 |
| antibacterial and antioxidant activities in sideritis italica (miller) greuter et burdet essential oils. | sideritis italica (miller) greuter et burdet is a widespread lamiacea in the mediterranean region used in traditional medicine. essential oils were antibacterial against nine atcc and as many clinically isolated gram-positive and gram-negative bacterial strains. antibacterial activity was also found against helicobacter pylori: a dose-dependant inhibition was shown between 5 and 25 microg/ml. the antibacterial activity of the oils was expressed as mics (minimum inhibitory concentrations) and mbc ... | 2006 | 16690235 |
| differential caspase-3 expression in noncancerous, premalignant and cancer tissues of stomach and its clinical implication. | caspase-3 is a critical apoptosis-promoting element but its status during stepwise gastrocarcinogenesis needs to be further clarified. | 2006 | 16697119 |
| erythrocyte lewis (a+b-) host phenotype is a factor with familial clustering for increased risk of helicobacter pylori-related non-cardiac gastric cancer. | the purpose of the present study was to test whether host erythrocyte lewis phenotypes correlated with the risk of gastric cancers. because of the association of gastric cancer with familial clustering, cancer relatives were investigated as to whether they had unique distribution of lewis phenotypes. | 2006 | 16724994 |
| oxidative phosphorylation and its coupling to mitochondrial creatine and adenylate kinases in human gastric mucosa. | energy metabolism in gastrobiopsy specimens of the antral and corpus mucosa, treated with saponin to permeabilize the cells, was studied in patients with gastric diseases. the results show twice lower oxidative capacity in the antral mucosa than in the corpus mucosa and the relative deficiency of antral mitochondria in complex i. the mucosal cells expressed mitochondrial and cytosolic isoforms of creatine kinase and adenylate kinase (ak). creatine (20 mm) and amp (2 mm) markedly stimulated mitoc ... | 2006 | 16741143 |
| helicobacter pylori inhibits expression of heat shock protein 70 (hsp70) in human epithelial cell line. importance of cag a protein. | heat shock proteins (hsp) are crucial for the maintenance of cell integrity under normal cell growth and at pathophysiological conditions such as colonization of gastric mucosa by helicobacter pylori (hp). the effect of hp on mrna expression for hsp70 in the gastric epithelial cells in vitro has been little studied and remains inconclusive. in this study we attempted to determine the alterations in gene expression for hsp70 induced by two live strains of hp in the epithelial mkn7 cells. the foll ... | 2006 | 16845230 |
| gene-expression profiles in gastric epithelial cells stimulated with spiral and coccoid helicobacter pylori. | human gastric epithelial immortalized ges-1 cells were infected with spiral and coccoid helicobacter pylori. scanning electron microscopy was used to determine the ability of the two forms of h. pylori to adhere to ges-1 cells. ges-1 cell apoptosis induced by coccoid and spiral h. pylori was analysed using flow cytometry. a cdna microarray for 22,000 human genes was used to identify the gene-expression differences in ges-1 cells infected with the two forms of h. pylori, and the gene expression i ... | 2006 | 16849720 |
| the stringent response is required for helicobacter pylori survival of stationary phase, exposure to acid, and aerobic shock. | the gastric pathogen helicobacter pylori must adapt to fluctuating conditions in the harsh environment of the human stomach with the use of a minimal number of transcriptional regulators. we investigated whether h. pylori utilizes the stringent response, involving signaling through the alarmone (p)ppgpp, as a survival strategy during environmental stresses. we show that the h. pylori homologue of the bifunctional (p)ppgpp synthetase and hydrolase spot is responsible for all cellular (p)ppgpp pro ... | 2006 | 16855239 |
| should the presence of polymorphisms of cyp2c19 enzymes influence the choice of the proton pump inhibitor for treatment of helicobacter pylori infection? | there are variations in the cyp2c19 genotypes, that are important for the metabolism of ppis. patients who are heterozygotes for the mutation, but especially homozygotes, have a much slower metabolism, which will result in more profound acid suppression. studies have been published, that suggest that the success rate of anti-helicobacter therapy is in part related to the cyp2c19 genotype of the patient. however, it is important to keep in mind that most studies that have evaluated this have been ... | 2006 | 16863548 |
| helicobacter pylori diagnosis and management. | h pylori is a global human pathogen and is the major cause of gastritis and the gastritis-associated diseases: gastric ulcer, duodenal ulcer, gastric cancer, and primary gastric b-cell lymphoma (maltoma). although several reliable diagnostic tests are widely available, the ideal regimen for treating the infection re-mains to be established. the current first-line or legacy triple therapy regimens fail in 20% to 40% of patients. causes of treatment failure include antibiotic resistance, poor comp ... | 2006 | 16880064 |
| helicobacter pylori eradication therapy on histologic change in the distal esophagus. | although cases of reflux esophagitis (re) developing after treatment to eradicate helicobacter pylori have been discussed in some detail, no reports are available concerning the histologic examination of re both before and after eradication therapy. | 2006 | 16882323 |
| demonstration and characterization of mutations induced by helicobacter pylori organisms in gastric epithelial cells. | helicobacter pylori gastritis increases gastric cancer risk. microsatellite instability-type mutations are secondary to deficient dna mismatch repair. h. pylori gastritis is more frequent in patients with microsatellite instability-positive gastric cancers, and h. pylori organisms independently of inflammation can reduce dna mismatch repair protein levels, raising the hypothesis that h. pylori organisms might lead to mutagenesis during infection. | 2006 | 16882331 |
| bioinformatic analysis of helicobacter pylori xgprtase: a potential therapeutic target. | xanthine-guanine phosphoribosyltransferase (xgprtase) is an enzyme of purine nucleotide salvage synthesis. the gpt gene of helicobacter pylori has been annotated as encoding an xgprtase and proposed as essential for survival of the bacterium in vitro. the aims of this work were to investigate the structure of h. pylori xgprtase and to compare the key features of the enzyme to other phosphoribosyltransferases employing computational, modelling, and bioinformatic tools. | 2006 | 16882332 |
| polymorphisms in th1-type cell-mediated response genes and risk of gastric cancer. | helicobacter pylori infection, the dominant risk factor for gastric cancers, has been shown to elicit t helper type 1 (th1) polarized immunological responses. we conducted a population-based study of 305 gastric cancer cases and 427 age- and gender-matched controls in warsaw, poland, to evaluate the association with several variants in genes responsible for th1-cell-mediated response. genotyping was performed on genomic dna by taqman(tm) assays to determine tnfa (-308 g>a, -417 g>a, -555 g>a, -1 ... | 2007 | 16885196 |
| a case of cutaneous t cell pseudolymphoma in a patient with helicobacter pylori infection. | cutaneous pseudolymphomas (cpl) are benign cutaneous lymphoproliferative infiltrations of various origin, including among others bacterial infections, viral infections and drugs . helicobacter pylori has been frequently founded in the stomach of patients with malt lymphoma. in january 2001, a 43-year-old man was referred to our department because of a 1-month history of itchy erythematous patches, plaques and flat tumors on his body. histological examination revealed nodular infiltrations compos ... | 2006 | 16902296 |
| function and recruitment of mucosal regulatory t cells in human chronic helicobacter pylori infection and gastric adenocarcinoma. | cd4(+)cd25(high) foxp3-expressing regulatory t cells (treg) can suppress immune responses to infections and tumors, thereby promoting microbial persistence and tumor progression. however, little is known about the phenotype and function of human mucosal treg. therefore, we analyzed the suppressive activity and homing phenotype of treg in gastric mucosa of helicobacter pylori-infected gastric adenocarcinoma patients. we found increased numbers of cd4(+)foxp3(+) treg in the tumor compared to tumor ... | 2006 | 16934529 |
| h pylori receptor mhc class ii contributes to the dynamic gastric epithelial apoptotic response. | to investigate the role of mhc class ii in the modulation of gastric epithelial cell apoptosis induced by h pylori infection. | 2006 | 16937440 |
| helicobacter pylori induces mono-(adenosine 5'-diphosphate)-ribosylation in human gastric adenocarcinoma. | mono-(adenosine 5'-diphosphate) (adp)-ribosylation, which transfers an adp-ribose from nicotinamide adenine dinucleotide (nad) to an acceptor protein, is an important post-translational modification of cellular proteins. several bacterial toxins are known to possess the mono-adp-ribosyltransferase activity to catalyze this reaction as a possible pathogenic factor. therefore, the aim of this study was to examine whether h. pylori may also induce mono-adp-ribosylation in a human gastric mucosal pr ... | 2006 | 16964392 |
| neuroimmune link in the mucosa of chronic gastritis with helicobacter pylori infection. | it is suggested that different neuropeptides regulate gastric mucosal integrity and participate in the development of chronic gastritis. the aim of this study was to examine the roles and changes of immunoreactive (ir) nerves and immunocompetent cells in human gastritis. immunohistochemical, immunocytochemical, and confocal laser microscopic methods were used. all investigated nerve fibers were found in different quantities in the mucosa of both control and gastritis samples. the number of sp, n ... | 2006 | 16983504 |
| human milk vacuolating cytotoxin a immunoglobulin a antibodies modify helicobacter pylori infection in gambian children. | we collected data, including the weights, urea breath test results, and presence of maternal milk cytotoxin-associated gene-specific and vacuolating cytotoxin a-specific immunoglobulin a monthly from 48 mothers and infants (to 44 weeks of age) in the gambia. in all, 11 children (23%) had negative urea breath test results, and 37 (77%) had positive results. weight loss associated with helicobacter pylori colonization was restricted to children whose mothers did not produce anti-vacuolating cytoto ... | 2006 | 16983617 |
| role of a muty dna glycosylase in combating oxidative dna damage in helicobacter pylori. | muty is an adenine glycosylase that has the ability to efficiently remove adenines from adenine/7,8-dihydro-8-oxoguanine (8-oxo-g) or adenine/guanine mismatches, and plays an important role in oxidative dna damage repair. the human gastric pathogen helicobacter pylori has a homolog of the muty enzyme. to investigate the physiological roles of muty in h. pylori, we constructed and characterized a muty mutant. h. pylori muty mutants incubated at 5% o2 have a 325-fold higher spontaneous mutation ra ... | 2007 | 16996809 |
| expression, purification and characterization of the membrane-associated hrca repressor protein of helicobacter pylori. | helicobacter pylori, a microaerophilic, gram-negative bacterium is a human pathogen that colonizes the gastric niche and is associated with several acute and chronic stomach diseases. in order to survive in the gastric environment and become pathogenic, the bacterium relies on a plethora of virulence factors, which also include heat shock proteins. we previously showed that two out of the three operons encoding the major cellular chaperone machineries are transcriptionally repressed by two regul ... | 2007 | 16997572 |
| helicobacter pylori vaca toxin promotes bacterial intracellular survival in gastric epithelial cells. | helicobacter pylori colonizes the gastric epithelium of at least 50% of the world's human population, playing a causative role in the development of chronic gastritis, peptic ulcers, and gastric adenocarcinoma. current evidence indicates that h. pylori can invade epithelial cells in the gastric mucosa. however, relatively little is known about the biology of h. pylori invasion and survival in host cells. here, we analyze both the nature of and the mechanisms responsible for the formation of h. p ... | 2006 | 17000720 |
| clinical application of pharmacogenetics in gastrointestinal diseases. | as knowledge of the human genome grows, there will be a direct impact on the management of specific diseases. within gastroenterology and hepatology, there has been a change in the understanding of how variations or mutations in genes involved in drug metabolism or disease pathophysiology affect response to therapy. this review discusses the application of clinical pharmacogenetics to the following diseases and disorders: inflammatory bowel disease, helicobacter pylori infections, gastroesophage ... | 2006 | 17020413 |
| clustering of helicobacter pylori vaca in lipid rafts, mediated by its receptor, receptor-like protein tyrosine phosphatase beta, is required for intoxication in az-521 cells. | helicobacter pylori vacuolating cytotoxin, vaca, induces multiple effects on epithelial cells through different cellular events: one involves pore formation, leading to vacuolation, mitochondrial damage, and apoptosis, and the second involves cell signaling, resulting in stimulation of proinflammatory responses and cell detachment. our recent data demonstrated that vaca uses receptor-like protein tyrosine phosphatase beta (rptpbeta) as a receptor, of which five residues (qttqp) at positions 747 ... | 2006 | 17030583 |
| detection of lactobacillus gasseri oll2716 strain administered with yogurt drink in gastric mucus layer in humans. | in animal models and human trials, lactobacillus gasseri oll2716 (lg21) strain suppressed helicobacter pylori colonization in the stomach. the aim of the present study was to clarify whether orally administered lg21 strain can enter the gastric mucus layer. biopsy samples were taken from the gastric antrum and corpus of two healthy volunteers (h. pylori infected and non-infected) who drank yogurt supplemented with lg21 strains. dna of lg21 and h. pylori in the mucus layer was detected using the ... | 2006 | 17032235 |
| effect of oral administration of bismuth compounds on campylobacter colonization in broilers. | bismuth compounds have been used since the 18th century to treat gastrointestinal ailments in man. colloidal bismuth subcitrate (de-nol) is currently used in combination with antibiotics to reduce enteric helicobacter pylori colonization as a treatment of stomach ulcers. we investigated whether bismuth citrate or its parent compound, colloidal bismuth subcitrate, would reduce colonization of the closely related foodborne pathogen, campylobacter jejuni in chickens. in 2 studies, birds were either ... | 2006 | 17032837 |
| interplay between helicobacter pylori and the immune system. clinical implications. | helicobacter pylori (h. pylori) is a gram-negative bacteria infecting more than 50% of human population. h. pylori selectively colonizes gastric mucosa and represents the major cause of gastroduodenal pathologies, such as gastric ulcer, autoimmune gastritis, gastric cancer and b cell lymphoma of mucosa associated lymphoid tissue (malt). in this review interplay between h. pylori and both innate and adaptive immune responses is discussed. the second part of this article presents current knowledge ... | 2006 | 17033103 |
| a population genetics pedigree perspective on the transmission of helicobacter pylori. | the inference of transmission pathways for medicinally important bacteria is important to our understanding of pathogens. here we report analyses of transmission in helicobacter pylori, a major carcinogen. our study is novel in that the focal community comprises detailed family pedigrees and has a high prevalence of h. pylori. to infer transmission, we performed high-resolution analyses of nucleotide sequences for three genes and accounted for the occurrence of mutation and recombination through ... | 2006 | 17057244 |
| gastric stump lymphoma after distal gastrectomy for benign peptic ulcer: report of a case. | we report a case of primary non-hodgkin gastric stump lymphoma, found in a 78-year-old man 30 years after a distal gastrectomy for a benign peptic ulcer. the development of lymphoma in the gastric stump is rare. in fact, to our knowledge only 37 cases, including this one, have been documented. although helicobacter pylori is thought to be a predisposing factor, we found no histological evidence of this infection in our patient. conversely, bile reflux and nitrite and n-nitrous compounds caused b ... | 2006 | 17072720 |
| resistance of primary murine cd4+ t cells to helicobacter pylori vacuolating cytotoxin. | persistent colonization of the human stomach by helicobacter pylori is a risk factor for the development of gastric cancer and peptic ulcer disease. h. pylori secretes a toxin, vaca, that targets human gastric epithelial cells and t lymphocytes and enhances the ability of h. pylori to colonize the stomach in a mouse model. to examine how vaca contributes to h. pylori colonization of the mouse stomach, we investigated whether murine t lymphocytes were susceptible to vaca activity. vaca inhibited ... | 2007 | 17074854 |
| age-dependent seroprevalence of antibodies against a helicobacter pylori-like organism and helicobacter pylori in commercially reared swine. | to determine the prevalence of antibodies against a swine-origin helicobacter pylori-like organism (hplo) and h pylori in conventionally reared swine. | 2006 | 17078751 |
| o-glycosylation of serum iga1 antibodies against mucosal and systemic antigens in iga nephropathy. | in iga nephropathy (igan), serum iga1 with abnormal o-glycosylation deposits in the glomerular mesangium. the underlying mechanism of this iga1 o-glycosylation abnormality is poorly understood, but recent evidence argues against a generic defect in b cell glycosyltransferases, suggesting that only a subpopulation of iga1-committed b cells are affected. for investigation of whether the site of antigen encounter influences iga1 o-glycosylation, the o-glycosylation of serum iga1 antibodies against ... | 2006 | 17093066 |
| identification of helicobacter pylori genes that contribute to stomach colonization. | chronic infection of the human stomach by helicobacter pylori leads to a variety of pathological sequelae, including peptic ulcer and gastric cancer, resulting in significant human morbidity and mortality. several genes have been implicated in disease related to h. pylori infection, including the vacuolating cytotoxin and the cag pathogenicity island. other factors important for the establishment and maintenance of infection include urease enzyme production, motility, iron uptake, and stress res ... | 2007 | 17101654 |
| initial 48-hour acid inhibition by intravenous infusion of omeprazole, famotidine, or both in relation to cytochrome p450 2c19 genotype status. | faster and stronger acid inhibition is required for the treatment of hemorrhage from peptic ulcers. we compared the effects of intravenous infusion regimens of a proton pump inhibitor (ppi) alone, a histamine 2 receptor antagonist (h2ra) alone, and the combination of a ppi with an h2ra on acid inhibition in the early postadministration phase in relation to cytochrome p450 (cyp) 2c19 genotype status. | 2006 | 17112810 |
| tlr4-dependent nf-kappab activation and mitogen- and stress-activated protein kinase 1-triggered phosphorylation events are central to helicobacter pylori peptidyl prolyl cis-, trans-isomerase (hp0175)-mediated induction of il-6 release from macrophages. | helicobacter pylori infection is associated with the local production of chemokines and cytokines, of which il-6 is overexpressed at the margin of gastric ulcer in h. pylori-positive gastritis. cells of the monocytic lineage are the major sources of il-6, and mononuclear cell infiltration in the lamina propria is characteristic of h. pylori-induced chronic infection. our study shows for the first time that a secreted peptidyl prolyl cis-, trans-isomerase, hp0175 elicits il-6 gene expression and ... | 2006 | 17114467 |
| interleukin-8 induction by helicobacter pylori in gastric epithelial cells is dependent on apurinic/apyrimidinic endonuclease-1/redox factor-1. | helicobacter pylori infection causes inflammation and increases the expression of il-8 in human gastric epithelial cells. h. pylori activates nf-kappab and ap-1, essential transcriptional factors in h. pylori-induced il-8 gene transcription. although colonization creates a local oxidative stress, the molecular basis for the transition from infection to the expression of redox-sensitive cytokine genes is unknown. we recently reported that the expression of apurinic/apyrimidinic endonuclease-1/red ... | 2006 | 17114472 |
| assay of human gastric mucin as a natural antibiotic against helicobacter pylori. | helicobacter pylori infects more than half of the world's population and is considered a leading cause of peptic ulcer and gastric carcinoma. although a large number of persons are infected with h. pylori, only a limited number of those infected (approximately 3%) develop peptic ulcers and gastric carcinoma. the progression of the disease is restricted by deeper portion of the gastric mucosa, and in many persons glandular atrophy appears to be prevented by mucins secreted in the deeper portion o ... | 2006 | 17116474 |
| trials of novel 13c-urea-containing capsule for more economic and sensitive diagnosis of helicobacter pylori infection in human subjects. | to develop a 13c-urea-containing capsule for more economic and sensitive diagnosis of helicobacter pylori infection, the 13c-urea-containing capsules were prepared with various additives such as polyethylene glycol, microcrystalline cellulose, sodium lauryl sulfate and citric acid. their dissolution test and 13c-urea breath test in human volunteers were then performed. polyethylene glycol increased the initial dissolution rates of urea and difference delta 13c values from 13c-urea, while microcr ... | 2006 | 17121183 |
| molecular mechanisms involved in anti-inflammatory effects of proton pump inhibitors. | interleukin (il)-8 has been reported to participate in neutrophil infiltration in helicobacter pylori (h. pylori)-induced gastritis in humans. in this study, we investigated the anti-inflammatory actions beyond the suppression of acid secretion by proton pump inhibitors (ppi), such as omeprazole and lansoprazole, on il-8 production by gastric epithelial cells (mkn45) and human umbilical vein endothelial cells (huvec) and on the transendothelial migration of polymorphonuclear neutrophils (pmn). | 2006 | 17122965 |
| interplay between helicobacter pylori and host gene polymorphisms in inducing oxidative dna damage in the gastric mucosa. | infection by helicobacter pylori is the most important risk factor for gastric cancer. however, only a small fraction of colonized individuals, representing at least half of the world's population, develop this malignancy. in order to shed light on host-microbial interactions, gastric mucosa biopsies were collected from 119 patients suffering from dyspeptic symptoms. 8-hydroxy-2'-deoxyguanosine (8-oxo-dg) levels in the gastric mucosa were increased in carriers of h.pylori, detected either by cul ... | 2007 | 17127715 |
| defensins and cathelicidins in gastrointestinal infections. | to review recently published studies presenting novel and relevant information on antimicrobial peptides in gastrointestinal infections. | 2007 | 17133082 |
| quantitative determination of gland mucous cells-type mucin using a monoclonal antibody, hik1083: its pathophysiological changes in human gastric juice. | pathological alteration in gastric mucosa is caused by helicobacter pylori infection and is detectable by histological analysis. in particular, the alteration of gland mucous cells (gmcs)-type mucin, which plays a protective role against h. pylori infection, is critical in the pathogenesis of h. pylori-related gastritis. we established an assay for gmcs-type mucin and quantitatively assessed the pathophysiological changes in its content in human gastric juice samples. | 2007 | 17134689 |
| the relationship between helicobacter pylori infection and promoter polymorphism of the nrf2 gene in chronic gastritis. | the transcription factor nrf2 regulates the expression of detoxifying and antioxidant genes. three polymorphisms of the nrf2 gene have been reported. we attempted to clarify the relationship between nrf2 gene polymorphism and chronic gastritis in a japanese population. the study was performed in 159 patients with no evidence of gastric malignancy on upper gastrointestinal endoscopy (mean age, 62.03 years; male:female ratio, 102:57; peptic ulcer diseases in 69 patients, and helicobacter pylori (h ... | 2007 | 17143558 |
| cytokine expression due to helicobacter pylori in a tissue culture model. | helicobacter pylori, in recent years, has been recognized as the major causative agent in chronic gastritis and peptic ulcer disease in humans. h. pylori is a ubiquitous organism, with at least half of the world's population infected. of those individuals with peptic ulcer disease, it is estimated that 90% of cases are caused by h. pylori. currently, the efficacy of therapies is starting to decline due to increasing resistance rates, especially towards clarithromycin. due to this, new therapies ... | 2007 | 17160356 |
| helicobacter pylori caga induces ags cell elongation through a cell retraction defect that is independent of cdc42, rac1, and arp2/3. | helicobacter pylori, which infects over one-half the world's population, is a significant risk factor in a spectrum of gastric diseases, including peptic ulcers and gastric cancer. strains of h. pylori that deliver the effector molecule caga into host cells via a type iv secretion system are associated with more severe disease outcomes. in a tissue culture model of infection, caga delivery results in a dramatic cellular elongation referred to as the "hummingbird" phenotype, which is characterize ... | 2007 | 17194805 |
| participation of capsaicin-sensitive afferent nerves in the gastric mucosa of patients with helicobacter pylori-positive or-negative chronic gastritis. | capsaicin-sensitive afferent nerves (csans) are involved in the protection of gastric mucosa. to clarify the role of csans in human helicobacter pylori-negative or -positive chronic gastritis, after bacterium detection by rapid urease test, (14)c urea breath test, and specific histological staining, the immunodistribution of capsaicin receptor, calcitonin gene-related peptide (cgrp), and substance p (sp) was studied in 21 h. pylori-positive and 30 h. pylori-negative patients with chronic gastrit ... | 2007 | 17195120 |
| eradication of helicobacter pylori might halt the progress to oesophageal adenocarcinoma in patients with gastro-oesophageal reflux disease and barrett's oesophagus. | | 2007 | 17196756 |
| helicobacter pylori-induced atrophic gastritis progressing to gastric cancer exhibits sonic hedgehog loss and aberrant cdx2 expression. | the loss of sonic hedgehog is an early change that occurs in the mucosa prior to neoplastic transformation and correlates with the type of intestinal metaplasia. aberrant expression of cdx has also been shown to correlate with the development of intestinal metaplasia. | 2006 | 17209848 |
| which has superior acid-suppressive effect, 10 mg omeprazole once daily or 20 mg famotidine twice daily? effects of single or repeated administration in japanese helicobacter pylori-negative cyp2c19 extensive metabolizers. | low-dose omeprazole is superior to full-dose famotidine in maintenance therapy for gastroesophageal reflux disease, whereas "on-demand" famotidine is more effective for relief of episodes of heartburn. to explain this apparent discrepancy, intragastric ph was measured for 24-hr seven times in eight japanese helicobacter pylori-negative cytochrome p450 2c19 extensive metabolizers; on days 1, 8, and 15 of repeated administration of 10 mg of omeprazole once daily and of 20 mg of famotidine twice da ... | 2007 | 17211705 |
| mucin expression in reactive gastropathy: an immunohistochemical analysis. | reactive gastropathy is the second most common diagnosis made on gastric biopsies. increased epithelial proliferation and modifications of epithelial cytokeratin profile, distinct from those of helicobacter pylori gastritis, have been previously reported. however, the evaluation of mucins, important components of the protective mucosal mucous layer, has not been reported. | 2007 | 17227128 |
| variant size- and glycoforms of the scavenger receptor cysteine-rich protein gp-340 with differential bacterial aggregation. | glycoprotein gp-340 aggregates bacteria in saliva as part of innate defence at mucosal surfaces. we have detected size- and glycoforms of gp-340 between human saliva samples (n = 7) and lung gp-340 from a proteinosis patient using antibodies and lectins in western blots and elisa measurements. western blots of saliva samples, and of gp-340 purified, from the seven donors using a gp-340 specific antibody distinguished four gp-340 size variants, designated i to iv (n = 2,2,2 and 1). while saliva g ... | 2007 | 17243023 |
| serological assays for identification of human gastric colonization by helicobacter pylori strains expressing vaca m1 or m2. | the helicobacter pylori vaca gene encodes a secreted protein (vaca) that alters the function of gastric epithelial cells and t lymphocytes. h. pylori strains containing particular vaca alleles are associated with differential risk of disease. because the vaca midregion may exist as one of two major types, m1 or m2, serologic responses may potentially be used to differentiate between patients colonized with vaca m1- or vaca m2-positive h. pylori strains. in this study, we examined the utility of ... | 2007 | 17267587 |
| cytochrome p450 2c19 polymorphism influences the preventive effect of lansoprazole on the recurrence of erosive reflux esophagitis. | the efficacy of lansoprazole (lpz) at inhibiting gastric acid secretion is influenced by cytochrome p450 2c19 (cyp2c19) polymorphism. the purpose of the present study was to investigate whether cyp2c19 polymorphism had an influence on the remission of erosive reflux esophagitis (re) during maintenance therapy with lpz. | 2007 | 17295875 |
| allergic sensitization and microbial load--a comparison between finland and russian karelia. | epidemiological data have indicated that some infections are associated with a low risk of allergic diseases, thus supporting the idea (hygiene hypothesis) that the microbial load is an important environmental factor conferring protection against the development of allergies. we set out to test the hygiene hypothesis in a unique epidemiological setting in two socio-economically and culturally markedly different, although genetically related, populations living in geographically adjacent areas. t ... | 2007 | 17302731 |
| [gastric relapse of stage i ocular adnexal mucosa-associated lymphoid tissue lymphoma]. | a 57-year-old male with right ocular adnexal mucosa-associated lymphoid tissue lymphoma (malt lymphoma) was successfully treated with local radiation therapy. the gastroendoscopic examination revealed a slight inflammatory change of the gastric mucosa, and the urease test was positive. eradication therapy against helicobacter pylori was successfully done, however, the patient developed gastric malt lymphoma two years after the initial treatment. southern blot analysis of the immunoglobulin heavy ... | 2007 | 17313077 |