| cure of helicobacter pylori infection by omeprazole-clarithromycin-based therapy in non-human primates. | rhesus monkeys raised in colonies tend to become naturally infected by helicobacter pylori early in life. earlier attempts to cure h. pylori infection with a 10-day triple therapy (metronidazole, amoxicillin, and peptobismol) were only partially (60%) successful, probably because of preexisting metronidazole resistance. this study was carried out to determine the efficacy of an alternative clarithromycin-omeprazole-based therapy for curing h. pylori infection in rhesus monkeys (macaca mulatta), ... | 1998 | 9497216 |
| the lactosylceramide binding specificity of helicobacter pylori. | the possible role of glycosphingolipids as adhesion receptors for the human gastric pathogen helicobacter pylori was examined by use of radiolabeled bacteria, or protein extracts from the bacterial cell surface, in the thin-layer chromatogram binding assay. of several binding specificities found, the binding to lactosylceramide is described in detail here, the others being reported elsewhere. by autoradiography a preferential binding to lactosylceramide having sphingosine/phytosphingosine and 2- ... | 1998 | 9499377 |
| mucosa-associated lymphoid tissue lymphoma coexisting with epithelioid granulomas in the stomach of a patient with systemic sarcoidosis. | malignant lymphoma arising in the stomach of a 23-year-old japanese man with systemic sarcoidosis is presented. the patient was followed because of systemic sarcoidosis involving the lungs, eyes, and lymph nodes. biopsy specimens from the stomach were repeated because of recurrent epigastralgia and multiple ulcerations. some of the specimens revealed epithelioid granulomas with no caseous necrosis, which confirmed gastric involvement of sarcoidosis. three years after the initial diagnosis, biops ... | 1997 | 9503470 |
| strand compositional asymmetry in bacterial and large viral genomes. | several bacterial genomes exhibit preference for g over c on the dna leading strand extending from the origin of replication to the ter-region in the genomes of escherichia coli, mycoplasma genitalium, bacillus subtilis, and marginally in haemophilus influenzae, mycoplasma pneumoniae, and helicobacter pylori. strand compositional asymmetry is not observed in the cyanobacterium synechocystis sp. genome nor in the archaeal genomes of methanococcus jannaschii, methanobacterium thermoautotrophicum, ... | 1998 | 9520433 |
| clinicopathological significance of altered loci of replication error and microsatellite instability-associated mutations in gastric cancer. | replication errors (rers) judged by microsatellite instability and its associated mutations have been recognized as an important mechanism in tumorigenesis of gastric cancers (gcs). to gain a deeper insight into its significance, we examined the frequency of rers using nine microsatellite markers and screened mutations in the polydeoxyadenine tract of the transforming growth factor beta type ii receptor gene (tgf-betarii) and polydeoxyguanine tracts of insulin-like growth factor ii receptor and ... | 1998 | 9537253 |
| high proportion of granzyme b-positive (activated) intraepithelial and lamina propria lymphocytes in lymphocytic gastritis. | intraepithelial lymphocytes (iels) and lamina propria lymphocytes (lpls) have not been well studied in gastric mucosa, particularly in lymphocytic gastritis. therefore, they were immunohistologically characterized with antibodies recognizing cd3, cd8, cd57, t cell-restricted intracellular antigen (tia-1), and granzyme b (grb). the tia-1 labels cytotoxic granules of resting and activated t-cells, whereas grb decorates activated cytotoxic t cells. thirty patients with celiac disease, including 20 ... | 1998 | 9537473 |
| simultaneous expression of type 1 and type 2 lewis blood group antigens by helicobacter pylori lipopolysaccharides. molecular mimicry between h. pylori lipopolysaccharides and human gastric epithelial cell surface glycoforms. | previous structural investigations performed on the lipopolysaccharides (lpss) from the human gastric pathogen helicobacter pylori have revealed that these cell surface glycan molecules express type 2 partially fucosylated, glucosylated, or galactosylated n-acetyllactosamine o antigen chains (o-chains) of various lengths, which may or may not be terminated at the nonreducing end by lewis x (lex) and/or ley blood group epitopes in mimicry of human cell surface glycoconjugates and glycolipids. sub ... | 1998 | 9565568 |
| helicobacter pylori is killed by nitrite under acidic conditions. | due to the expression of urease, helicobacter pylori is able to establish itself in the human stomach under acidic conditions. a novel host defence mechanism was recently proposed, suggesting that the formation of salivary nitrite in symbiosis with facultative anaerobic bacteria in the oropharynx, is aimed at enhancing the antimicrobial activity of gastric juice. | 1998 | 9577337 |
| screening system for urease inhibitors using 13c-nmr. | urease inhibitors are candidate drugs to treat infection with the human pathogen, helicobacter pylori, which produces a potent urease [urea amidohydrolase; ec 3.5.1.5]. we developed a screening system based on 13c-nmr measurement of the time course of decrease in the signal of 13c-urea in the presence of urease. the effect on urease activity of known inhibitors, hydroxamic acids, l-ascorbic acid, 2,2'-dipyridyl disulfide and ninhydrin, was speedily and conveniently measured by this method. | 1998 | 9579048 |
| comparison of enhanced elimination of bismuth in humans after treatment with meso-2,3-dimercaptosuccinic acid and d,l-2,3-dimercaptopropane-1-sulfonic acid. | two groups of 12 human volunteers, who had been treated with colloidal bismuth subcitrate, because of helicobacter pylori-associated gastritis, participated in the study. the patients received a single dose of meso-2,3-dimercaptosuccinic acid (dmsa) or d,l-2,3-dimercaptopropane-1-sulfonic acid (dmps) at a dose of 30 mg kg-1 in a randomized single blind study. in contrast to dmps, increasing concentrations of bismuth in blood were observed during the first 4 h after intake of dmsa. in urine, both ... | 1998 | 9581027 |
| human antibody response to helicobacter pylori lipopolysaccharide: presence of an immunodominant epitope in the polysaccharide chain of lipopolysaccharide. | we have examined the antibody response to helicobacter pylori lipopolysaccharides (lps) in humans. we used sera from patients with gastroduodenal diseases and healthy adults infected or not infected with h. pylori. data from the experiments for antibody binding to lps suggested that the polysaccharide chains from many h. pylori strains showed high immunogenicity in humans. sera from most (above 70%) h. pylori-infected individuals contained immunoglobulin g (igg) antibodies against the polysaccha ... | 1998 | 9596783 |
| loss of ps2 protein expression is an early event of intestinal-type gastric cancer. | to investigate the prevalence of ps2 expression in gastric cancer with respect to tumor histopathology, intestinal metaplasia and helicobacter pylori (h. pylori) infection, pathologic specimens of 91 patients with gastric cancer were immunostained for ps2. such immunoreactivity was correlated with the status of h. pylori infection, tumor staging, histology, subtyping, and associated intestinal metaplasia. positive ps2 staining was seen throughout all non-neoplastic epithelia, and in all 9 patien ... | 1998 | 9600121 |
| non-gastrointestinal consequences of helicobacter pylori infection. | evidence relating h. pylori to non-gastrointestinal disease is sparse and inconclusive. suggested mechanisms whereby infection might increase cardiovascular risk include release of acute-phase reactants including fibrinogen, reduction of hdl cholesterol, elevation of homocysteine levels and immunological cross-reactivity between bacterial and human heat shock proteins. six published studies relating h. pylori seropositivity to various measures of ischaemic heart disease (ihd)-angiography, acute ... | 1998 | 9604434 |
| helicobacter pylori eradication cost-benefit: the case against. | helicobacter pylori is an important human pathogen, implicated in the pathogenesis of peptic ulcer disease, gastric cancer, and perhaps also in other non-gastrointestinal disease. there is little doubt that in peptic ulcer disease at least, there is a strong argument for its eradication on both clinical and economic terms. the majority of subjects infected with helicobacter pylori never develop clinically overt disease, and it is this group that the clinical and financial benefit of eradication ... | 1998 | 9604448 |
| helicobacter pylori culture supernatant interferes with epidermal growth factor-activated signal transduction in human gastric kato iii cells. | the mechanisms by which helicobacter pylori infection leads to gastroduodenal ulceration remain poorly understood. previous studies have shown that h. pylori vacuolating cytotoxin (vaca) inhibits proliferation of gastric epithelial cells, which suggests that h pylori may interfere with gastric mucosal repair mechanisms. in this study, we investigated the effects of h. pylori broth culture supernatants on epidermal growth factor (egf)-mediated signal transduction pathways in a gastric carcinoma c ... | 1998 | 9626065 |
| antigen-dependent progression of mucosa-associated lymphoid tissue (malt)-type lymphoma in the stomach. effects of antimicrobial therapy on gastric malt lymphoma in mice. | in humans, low-grade b-cell mucosa-associated lymphoid tissue (malt) lymphomas of the stomach regress when helicobacter pylori infection is cured by antimicrobial therapy. using an animal model of human gastric malt lymphoma, we observed the effects of helicobacter felis eradication and the relationship between infection and disease progression. antimicrobial therapy was given to one-half of the balb/c mice infected with h. felis for 20 months. groups of antibiotic-treated and untreated mice wer ... | 1998 | 9626066 |
| circulating antibodies to the 60-kd heat shock protein (hsp) family in patients with helicobacter pylori infection. | whilst the mechanism by which helicobacter pylori causes different gastroduodenal diseases is uncertain, strains producing the cytotoxin-associated protein (caga) have greater pathogenicity. hsps are immunogenic molecules induced by inflammatory mediators. the aim of this study was to assess pathogenicity of hsp antibodies in h. pylori-infected patients. elisa techniques were used to assay sera of h. pylori-positive patients with gastritis, gastric atrophy, duodenal or gastric ulcer, and h. pylo ... | 1998 | 9649220 |
| induction of b cell responses in the stomach of helicobacter pylori- infected subjects after oral cholera vaccination. | we have evaluated the possibility of inducing antibody responses locally in the human stomach as a prerequisite for the development of a vaccine against helicobacter pylori. both h. pylori-infected and noninfected subjects were immunized with an oral b subunit whole cell (bs-wc) cholera vaccine, and total and vaccine-specific antibody-secreting cells (asc) were determined by the enzyme-linked immunospot (elispot) technique in cells isolated from the antrum and duodenum, respectively, before and ... | 1998 | 9649556 |
| the efficacy of antimicrobial mouth rinses in oral health care. | there is growing public recognition of the importance of oral health, as symbolized by the theme. "oral health for a healthy life" proposed for the 1994 world health day. in this report, the efficacy of antimicrobial mouth rinses, mainly listerine, was reviewed by three investigators who are working as a microbiologist, a microbiologist, a dentist, and a dental hygienist participating in oral health care. listerine, an antimicrobial mouth rinse, completely killed microorganisms in 10 to 30 secon ... | 1998 | 9663026 |
| gastric diseases related to helicobacter pylori and epstein-barr virus infection. | | 1998 | 9688075 |
| protective function of proteins and lipids in human milk. | human milk provides the infant with protection against infectious diseases. this protection is conferred through several mechanisms: specific antibody targeted protection against pathogens in the infant's environment (through milk iga, igg, and igm) and broad-spectrum, nonspecific protection provided through several distinct mechanisms. these are: bactericidal effects (lactoferrin), bacteriostatic action (lactoferrin, lysozyme), lysis of microorganisms (lysozyme), antiviral effects (lactoferrin, ... | 1998 | 9691157 |
| relapsed duodenal ulcer after cure of helicobacter pylori infection. | we report a patient--a 42-year-old man--who had suffered from recurrent duodenal ulcer for about 20 years. successful curative therapy for helicobacter pylori infection was performed for 2 weeks with new triple omeprazole, anoxicillin, clarithromycin (oac) treatment in october 1995, and cure of the infection was repeatedly confirmed by histology, culture, and the 13c urea breath test. one month after the curative therapy, recurrence of a small duodenal ulcer was observed and in february another ... | 1998 | 9719242 |
| localization of inducible nitric oxide synthase mrna in inflamed gastrointestinal mucosa by in situ reverse transcriptase-polymerase chain reaction. | immunohistochemistry has been critical in determining the tissue localization of inducible nitric oxide synthase (inos). however, this technique suffers from nonspecific staining which may lead to false-positive results and the failure of antisera to recognize inos from different species. we developed a technique to determine the localization of inos mrna, as opposed to protein, in tissue sections using an in situ rt-pcr (is rt-pcr) technique. sections of inflamed gastrointestinal mucosa were us ... | 1998 | 9731636 |
| experimental infection of mongolian gerbils with wild-type and mutant helicobacter pylori strains. | experimental helicobacter pylori infection was studied in mongolian gerbils with fresh human isolates that carry or do not carry caga (caga-positive or caga-negative, respectively), multiply passaged laboratory strains, wild-type strain g1.1, or isogenic urea, caga, or vaca mutants of g1.1. animals were sacrificed 1 to 32 weeks after challenge, the stomach was removed from each animal for quantitative culture, urease test, and histologic testing, and blood was collected for antibody determinatio ... | 1998 | 9746590 |
| helicobacter pylori infection, oxidative dna damage, gastric carcinogenesis, and reversibility by rebamipide. | several epidemiological studies have demonstrated a close association between helicobacter pylori infection and carcinoma of the mid- or distal stomach. if this can be shown to be a causal association, eradication of the organism may prevent later development of cancer. several mechanisms have been proposed by which h. pylori infection might lead to predisposition for gastric cancer. although many potential pathogenic mechanisms, such as increased proliferative gastric epithelial response to h. ... | 1998 | 9753230 |
| characterization of a helicobacter pylori vaccine candidate by proteome techniques. | in a previous two-dimensional (2d) gel electrophoretic study of protein antigens of the gastric pathogen, helicobacter pylori recognized by human sera, one of the highly and consistently reactive antigens, a protein with mr of approximately 30,000 (spot 15) seemed to be of special interest because of low yields on n-terminal protein sequencing. this suggested possible n-terminal modification, as the n-terminal sequence analysis of this 30,000 protein (spot 15) did not provide a definitive match ... | 1998 | 9766873 |
| helicobacter pylori up-regulates cyclooxygenase-2 mrna expression and prostaglandin e2 synthesis in mkn 28 gastric mucosal cells in vitro. | helicobacter pylori has been suggested to play a role in the development of gastric carcinoma in humans. also, mounting evidence indicates that cyclooxygenase-2 overexpression is associated with gastrointestinal carcinogenesis. we studied the effect of h. pylori on the expression and activity of cyclooxygenase-1 and cyclooxygenase-2 in mkn 28 gastric mucosal cells. h. pylori did not affect cyclooxygenase-1 expression, whereas cyclooxygenase-2 mrna levels increased by 5-fold at 24 h after incubat ... | 1998 | 9786845 |
| involvement of the cd95 (apo-1/fas) receptor and ligand system in helicobacter pylori-induced gastric epithelial apoptosis. | helicobacter pylori infection is associated with chronic gastritis, peptic ulceration, and gastric carcinoma. the potential role of cd95-mediated apoptosis was investigated in a panel of gastric biopsies obtained from patients with h. pylori-associated chronic gastritis (n = 29) and with noninfected normal mucosa (n = 10). immunohistochemistry revealed increased cd95 receptor expression in epithelial and lamina propria cells in chronic gastritis. by in situ hybridization, cd95 ligand mrna was ab ... | 1998 | 9788963 |
| previous infection with helicobacter pylori is the primary determinant of spontaneous gastric hypoacidity in human immunodeficiency virus-infected outpatients. | to investigate the incidence and demographics of gastric hypoacidity among persons infected with human immunodeficiency virus (hiv), 146 asymptomatic subjects were evaluated with use of a radiotelemetry device (heidelberg capsule). gastric hypoacidity (minimum gastric ph of > or = 3) occurred in 24 subjects (17%). demographic characteristics, cd4 cell counts, and helicobacter pylori serological status were evaluated for an association with gastric ph. subjects with hypoacidity were more likely t ... | 1998 | 9798026 |
| [using polymerase chain reaction to detect helicobacter heilmannii in gastric biopsy materials]. | helicobacter heilmannii is one of the species of helicobacter other than helicobacter pylori noticed in human gastric mucosa. in our study, a polymerase chain reaction (pcr) was designed for the detection of helicobacter heilmannii in gastric biopsy specimens. two broad-range bacterial 16s rdna primers and one helicobacter genus-specific 16s rdna primer were matched for detecting helicobacter genus bacteria. a hp-specific primer pair was used simultaneously to exclude the presence of hp. the pcr ... | 1997 | 9812529 |
| identification and functional importance of il-1 receptors on rat parietal cells. | we studied the expression of interleukin-1 (il-1) receptors and the effect of il-1beta on the function of highly enriched (>97%) rat parietal cells. rt-pcr of parietal cell poly(a)+ rna with primers specific for the rat il-1 receptor revealed a single 547-kb pcr product highly homologous to the published sequence of the il-1 receptor. northern blot analysis of poly(a)+ rna of rat parietal cells and brain revealed a single rna species of 5.7 kb. cytochemistry of parietal cell il-1 receptor was pe ... | 1998 | 9815040 |
| cyp2c19 genotype and phenotype determined with omeprazole in patients with acid-related disorders with and without helicobacter pylori infection. | omeprazole is to a major extent metabolized by cytochrome p450 isozyme cyp2c19. the aims of this study were to compare the phenotype of cyp2c19 determined by omeprazole with the genotype and to determine the effect of helicobacter pylori infection on the metabolism of omeprazole. | 1998 | 9829356 |
| [the infection theory in atherosclerosis]. | atherosclerosis displays all the features of a chronic inflammatory process. aggressions that ignite and fuel atherosclerotic inflammation warrant keen attention. infection is a potential clue, implying microbes with certain discrete characteristics: a wide epidemiologic distribution, a tropism for the arterial wall, and an aptitude for persistence, latency and recurrence. the infectious theory has built up from the pioneering observations of fabricant et al. (1978) on the arterial lesions provo ... | 1998 | 9833076 |
| relationship between expression of hsp60, urease activity, production of vacuolating toxin, and adherence activity of helicobacter pylori. | the relationship between the expression of heat shock protein 60 (hsp60) of helicobacterpylori on the bacterial surface and its pathogenic factors of urease activity, production of vacuolating toxin, and adherence to human gastric carcinoma cells was examined. there was no correlation between urease activity, production of vacuolating toxin, and the expression of hsp60. however, there was a significant correlation between the adherence activity to human gastric carcinoma mkn45 cells and the expr ... | 1998 | 9840008 |
| toxigenicity of helicobacter pylori isolates possessing caga gene and vacuolating cytotoxin. | inflammatory cells in the gastric epithelium and direct cell damage are important effects of h. pylori infection in causing gastroduodenal diseases. the aim of this study was to determine whether h. pylori possessing the caga gene and vacuolating cytotoxin activity (caga+/tox+) has these toxigenicities. the detection of caga was performed by the polymerase chain reaction method. culture supernatants of h. pylori were tested on rabbit gastric epithelial cell culture for intracellular vacuolation. ... | 1998 | 9840010 |
| utility of endoscopic biopsy samples to quantitate human duodenal ion transport. | duodenal mucosal bicarbonate secretion (dmbs) prevents acid-peptic damage and facilitates nutrient absorption. dmbs is diminished in patients with duodenal ulcers and is normalized after helicobacter pylori eradication. the measurement of dmbs in human patients in vivo requires intubation with a multi-lumen balloon tube and permits limited testing with putative agonists and antagonists. our purpose was to develop a means to investigate transport events in human duodenal biopsy samples in vitro. ... | 1998 | 9851742 |
| the relation between gastric vitamin c concentrations, mucosal histology, and caga seropositivity in the human stomach. | vitamin c may be protective against gastric cancer though infection with helicobacter pylori is associated with a reduction in intragastric concentrations of vitamin c. | 1998 | 9863475 |
| genes from nine genomes are separated into their organisms in the dinucleotide composition space. | a set of 16 kinds of dinucleotide compositions was used to analyze the protein-encoding nucleotide sequences in nine complete genomes: escherichia coli, haemophilus influenzae, helicobacter pylori, mycoplasma genitalium, mycoplasma pneumoniae, synechocystis sp., methanococcus jannaschii, archaeoglobus fulgidus, and saccharomyces cerevisiae. the dinucleotide composition was significantly different between the organisms. the distribution of genes from an organism was clustered around its center in ... | 1998 | 9872449 |
| analysis of 3- and 6-linked sialic acids in mixtures of gangliosides using blotting to polyvinylidene difluoride membranes, binding assays, and various mass spectrometry techniques with application to recognition by helicobacter pylori. | a convenient approach to analyze 3- and 6-linked sialic acids in mixtures of biologically active gangliosides was developed. the procedure was adapted to work on small amounts of material and included parallel tests, which allowed direct analysis of structure and activity. the initial step in the procedure was separation of a mixture of gangliosides by thin-layer chromatography (tlc) and blotting to a polyvinylidene difluoride membrane. the gangliosides were then analyzed (a) by direct desorptio ... | 1998 | 9882401 |
| [fas/fas ligand mrna are expressed in helicobacter pylori infected mucosa]. | helicobacter pylori (hp) seems to possess an inflammatory as well as immun-modulatory potential. the investigation revealed that both fas and fasligand are expressed in the gastric mucosa independently of the hp infection status and with no significant difference to the expression profile in pbmc. in conclusion, fas-fasligand interaction could play a role in immune regulation of hp gastritis and induction or mediation of epithelial injury. | 1998 | 9931819 |
| adenocarcinoid of ileum and appendix, incidentally discovered during exploratory laparotomy for gastric malt lymphoma, with subsequent diffuse prostatic metastases: report of a case with light, immunohistochemical, and electron microscopic studies. | the diagnosis of adenocarcinoid (mucinous/goblet cell carcinoid) is usually unexpected by both clinicians and pathologists. we report here the case of a 74-year-old man with gastric lymphoma (b-cell maltoma) diagnosed by endoscopy, who was found on exploratory laparotomy also to have extensive intraabdominal involvement by adenocarcinoid, arising from the ileum and/or appendix. the patient died two years after diagnosis with bladder outlet and small bowel obstruction due to diffuse metastases. i ... | 1999 | 9952228 |
| emergence of recombinant strains of helicobacter pylori during human infection. | genetic recombination can be important evolutionarily in speeding the adaptation of organisms to new environments and in purging deleterious mutations. here, we describe polymerase chain reaction (pcr), hybridization and dna sequence-based evidence of six such exchanges between two strains of helicobacter pylori during natural mixed infection of a patient in lithuania. one parent strain contained the 37 kb long, virulence-associated cag pathogenicity island (pai), and the other strain lacked thi ... | 1999 | 9987107 |
| biological activities of 1,1,6-trisubstituted indanes: beyond magainin 2. | msi-78 is a peptide analog of naturally occurring magainin 2 isolated from the skin of xenopus laevis. the peptide is known to have one of the strongest antibacterial activities in magainin 2 analogs against methicillin-resistant staphylococcus aureus (mrsa). to find novel compounds superior to msi-78, we have further designed, synthesizing 1,1-di(4-aminobutyl)-6-benzylindane (pm4) and 1,1-dibenzyl-6-(4-aminobutyl) indane (pm5), and tested their inhibitory ability of the growth of s. aureus. in ... | 1999 | 9989665 |
| omeprazole attenuates neutrophil-endothelial cell adhesive interaction induced by extracts of helicobacter pylori. | in this study, the influence of omeprazole on the adhesive activity of neutrophils, provided by an extract of helicobacter pylori, was determined. human neutrophils were collected from peripheral blood and labelled with a fluorochrome. helicobacter pylori (nctc 11637) was cultured and its water extract was obtained by centrifugation of the bacterial suspension. neutrophils were incubated with the extract in a plastic plate. percentage adherence was calculated by measuring the fluorescence of flo ... | 1999 | 10029274 |
| changes in the evolution of the antigenic profiles and morphology during coccoid conversion of helicobacter pylori. | the significance of the coccoid forms of h. pylori is still controversial and the questions of whether these forms are viable and infective or degenerative are still open. we induced conversion from rod to coccoid forms and studied morphological changes and antigenic evolutions during this conversion and, thereby, elucidated the viability of coccoid forms. | 1999 | 10063308 |
| [resuscitation from the viable but nonculturable state of helicobacter pylori]. | it is well known that the change of state from the culturable to not culturable on pathogenic organisms could easily occurred. these states of bacteria were so called viable but nonculturable (vnc). the coccoid form of h. pylori is seems to be in this state. the possibility of resuscitation of h. pylori was examined in vitro. the coccoid form bacteria was treated with ammonium sulfate and heat shock before culture, and cultured in brucella broth containing, sodium pyruvate, laked human erythrocy ... | 1999 | 10077897 |
| the tricarboxylic acid cycle of helicobacter pylori. | the composition and properties of the tricarboxylic acid cycle of the microaerophilic human pathogen helicobacter pylori were investigated in situ and in cell extracts using [1h]- and [13c]-nmr spectroscopy and spectrophotometry. nmr spectroscopy assays enabled highly specific measurements of some enzyme activities, previously not possible using spectrophotometry, in in situ studies with h. pylori, thus providing the first accurate picture of the complete tricarboxylic acid cycle of the bacteriu ... | 1999 | 10091606 |
| improving the success of culturing helicobacter pylori from gastric biopsies. | factors influencing the successful isolation of helicobacter pylori from human gastric biopsies were studied. within 24 h, each of the gastric biopsies was inoculated onto chocolate blood agar media and incubated for up to 2 weeks. among 63 (70%) culture positive cases in 90 patients, 58 (64%) cases were culture positive for both specimens, while five (6%) cases were culture positive in only one biopsy. of the 63 positive cultures, 51 h. pylori strains (81%) grew on both media with and without a ... | 1998 | 10093230 |
| mitogenic autoantibodies in helicobacter pylori-associated stomach cancerogenesis. | colonization of the bacterium helicobacter pylori of gastric mucosa plays an important role in stomach carcinogenesis, while the gastric mucosa and nearby lymphoid tissue are active sites of humoral immunity against both bacteria and tumor. in a broad study on the humoral immunity of stomach-cancer patients (5 patients with diffuse- and intestinal-type stomach carcinoma), we immortalized spleen cells by using human hybridoma technology and isolated 11 hybrid clones (9 igm, 1 igg and 1 iga) which ... | 1999 | 10188724 |
| the attenuated salmonella vaccine approach for the control of helicobacter pylori-related diseases. | the gram-negative bacterium helicobacter pylori is a widespread human pathogen that colonizes the gastric mucosa and is associated with gastro-intestinal illnesses such as gastritis, peptic ulcer, gastric lymphoma and gastric cancer. current pharmacological therapies are becoming less reliable for the control of h. pylori due to the elevated costs and to the increasing number of antibiotic resistant strains. new vaccination strategies utilizing h. pylori antigens combined with adjuvants or deliv ... | 1999 | 10194821 |
| expression of bcl-2 in autoimmune and helicobacter pylori-associated atrophic gastritis. | chronic atrophic gastritis can be induced either by h. pylori or by an autoimmune process. the protein product of bcl-2, which is a protooncogene, blocks apoptosis. aberrant bcl-2 expression has been found in 68% of atrophic gastritis patients. the aim of this study was to compare bcl-2 expression in 20 autoimmune atrophic gastritis patients to that in 20 h. pylori-associated atrophic gastritis patients. twenty patients with h. pylori antral gastritis but without atrophy served as controls. the ... | 1999 | 10219822 |
| can nsaid/asa-induced erosions of the gastric mucosa be identified at histology? | studies in animals have shown that nsaid/asa-induced erosions have an ischaemic pathogenesis. we therefore studied the question of whether such erosions in human gastric biopsy material can be identified on the basis of the ischaemic necrosis. histological sections prepared from forceps biopsy material obtained from 122 patients with erosions (at least three biopsy specimens from the erosion and two from antrum and corpus each) were classified by a pathologist blinded to the endoscopic findings ... | 1999 | 10220792 |
| hypothesis: the changing relationships of helicobacter pylori and humans: implications for health and disease. | helicobacter pylori has apparently colonized the human stomach since time immemorial and is superbly adapted for persistence. several genotypes, including cag+, are associated with increased risk of gastric and duodenal diseases. with modern life, for probably the first time in human history, there are large numbers of noncolonized persons. duodenal ulceration has been present essentially for only 200 years; that its incidence rose just as h. pylori was waning is best explained by changes in gas ... | 1999 | 10228075 |
| immunomodulation of helicobacter infection. | helicobacter pylori leads to a chronic infection in humans that is associated with gastric inflammation and a vigorous immune response. despite the humoral and cellular responses that can be detected in both human and animal models of helicobacter infection, the immune response fails to eliminate the organism. eradication failure may be due to the niche in which h pylori confines itself, well away from direct contact with elements of the immune system. alternatively, the general tendency of the ... | 1999 | 10331935 |
| glycosaminoglycan-binding microbial proteins in tissue adhesion and invasion: key events in microbial pathogenicity. | glycosaminoglycans such as heparin, heparan sulphate and dermatan sulphate, are distributed widely in the human body. several glycosaminoglycans form part of the extracellular matrix and heparan sulphate is expressed on all eukaryotic surfaces. the identification of specific binding to different glycosaminoglycan molecules by bacteria (e.g., helicobacter pylori, bordetella pertussis and chlamydia trachomatis), viruses (e.g., herpes simplex and dengue virus), and protozoa (e.g., plasmodium and le ... | 1999 | 10334589 |
| phenotypic changes of helicobacter pylori components during an experimental infection in mice. | the bacterial pathogen helicobacter pylori is highly adapted to the human stomach and the clinical isolates show a high diversity which could be due to adaptative changes of the strains passing from one host to another. in order to study these variations, experimental infection of mice was developed and provided three out of the eleven tested strains able to infect c57bl/6 mice: the sydney strain which is known to be well adapted to mice and two freshly isolated strains from infected patients. m ... | 1999 | 10340709 |
| cyp2c19 genotype status and effect of omeprazole on intragastric ph in humans. | omeprazole is metabolized by genetically determined s-mephenytoin 4'-hydroxylase (cyp2c19) in the liver. this study aimed to determine whether the effect of omeprazole on intragastric ph depends on cyp2c19 genotype status. | 1999 | 10340921 |
| a bacteriophage encoding a pathogenicity island, a type-iv pilus and a phage receptor in cholera bacteria. | the virulence properties of many pathogenic bacteria are due to proteins encoded by large gene clusters called pathogenicity islands, which are found in a variety of human pathogens including escherichia coli, salmonella, shigella, yersinia, helicobacter pylori, vibrio cholerae, and animal and plant pathogens such as dichelobacter nodosus and pseudomonas syringae. although the presence of pathogenicity islands is a prerequisite for many bacterial diseases, little is known about their origins or ... | 1999 | 10360577 |
| effects of helicobacter pylori on proliferation of gastric epithelial cells in vitro. | h. pylori infection of the gastric mucosa has been associated with an increase in gastric epithelial cell proliferation. however, in vitro adherence of h. pylori to gastric epithelial cells is associated with reduced cell proliferation. reduction of epithelial cell proliferation may contribute to ulcer formation and delay ulcer healing. the following study was undertaken to elucidate the ability of caga-positive and -negative strains to impede gastric epithelial cell proliferation. | 1999 | 10364015 |
| fingerprinting of helicobacter pylori strains by matrix-assisted laser desorption/ionization mass spectrometric analysis. | helicobacter pylori is an important human gastrointestinal pathogenic bacterium which is believed to colonize approximately one-half of the world's population. different strains of h. pylori possess virulence proteins for tissue colonization, host evasion and tissue damage. the bacteria display genomic instabilities that include gene rearrangements and gene exchange. recently, methods for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms) have been establi ... | 1999 | 10368980 |
| [the concept of the relationship between the human body and helicobacter pylori]. | | 1999 | 10380463 |
| presence of antibodies against helicobacter pylori and its heat-shock protein 60 in the serum of patients with sjögren's syndrome. | helicobacter pylori infection elicits a local and systemic immune response against bacterial antigens, including a heat-shock protein of 60 kda (hsp60). the homology between microbial and human hsp suggests that the immune response to bacterial hsp may play a role in the pathogenesis of autoimmune disorders. since gastric involvement and h. pylori have been reported in sjögren's syndrome (ss), we investigated the prevalence of antibodies against h. pylori and its specific hsp60 in sera from pati ... | 1999 | 10381048 |
| enhanced expression of inducible nitric oxide synthase and nitrotyrosine in gastric mucosa of gastric cancer patients. | recent studies (k. komoto et al., am. j. gastroenterol., 93: 1271-1276, 1998) have shown that helicobacter pylori infection is associated with gastric cancer. however, the mechanism of h. pylori in carcinogenesis has not been clarified. h. pylori infection leads to a sustained production of reactive nitrogen species that may contribute to cause dna damage. in this study, we examined the expression of inducible nitric oxide synthase (inos) and nitrotyrosine in gastric mucosa. the expression of in ... | 1999 | 10389926 |
| [helicobacter heilmannii: anatomo-clinical study of 14 new cases]. | introduction: helicobacter pylori is one of the most common causes of human gastritis. recently, a new agent has been isolated, which also causes a gastritis. it has been initially named gastrospirillum hominis and renamed helicobacter heilmannii (hh). hh is extremely rare. in spite of the rarity it is important to recognize and diagnose it, as it requires a proper therapy, different from hp therapy. clinical presentation and serological results of hh are superimposable to those of hp. therefore ... | 1999 | 10396946 |
| helicobacter pylori in the canadian arctic: seroprevalence and detection in community water samples. | many north american arctic communities are characterized by risk markers associated with helicobacter pylori (h. pylori) infection, including overcrowded housing and inadequate water supply and sanitation systems. our aim was to determine the seroprevalence of h. pylori infection in two traditional inuit communities in the central canadian arctic and to test for the presence of h. pylori, by polymerase chain reaction (pcr), in local water supplies. | 1999 | 10406242 |
| diagnosis of helicobacter pylori infection with a new non-invasive antigen-based assay. hpsa european study group. | helicobacter pylori is a common human pathogen implicated in certain gastrointestinal diseases. in the search for new non-invasive techniques to diagnose h. pylori infection, we evaluated an eia for h. pylori antigen in stool (hpsa). | 1999 | 10406362 |
| lymphoid proliferations and lymphomas associated with gastric metaplasia, dysplasia, and carcinoma. | gastric carcinomas are invariably accompanied by lymphoid proliferations. we studied their features in 22 resected gastric carcinomas in which the lymphoid proliferations ranged from reactive lymphoid follicles to mucosa-associated lymphoid tissue (malt) lymphomas. in most cases, the collections of lymphocytes were abundant, which is remarkable considering the lack of lymphoid tissue in the normal stomach. they were not haphazardly located but in direct contact with the metaplastic, dysplastic, ... | 1999 | 10414503 |
| helicobacter pylori induces gastric epithelial cell apoptosis in association with increased fas receptor expression. | the mechanisms involved in mediating the enhanced gastric epithelial cell apoptosis observed during infection with helicobacter pylori in vivo are unknown. to determine whether h. pylori directly induces apoptosis of gastric epithelial cells in vitro and to define the role of the fas-fas ligand signal transduction cascade, human gastric epithelial cells were infected with h. pylori for up to 72 h under microaerophilic conditions. as assessed by both transmission electron microscopy and fluoresce ... | 1999 | 10417197 |
| analysis of p53 gene mutations in helicobacter pylori-associated gastritis mucosa in endoscopic biopsy specimens. | no previous report has shown the relationship between helicobacter pylori infection and a direct sequence analysis of p53 gene mutation in a biopsy sample of human gastric mucosa. | 1999 | 10423062 |
| from plaque biology to clinical setting. | coronary atherosclerosis may cause acute and chronic ischemic syndromes; the former are caused by "acute plaque events," mostly thrombosis complicating vulnerable ruptured plaques, namely severe lesions with large core, thin cap, and weak shoulder infiltrated by activated inflammatory cells. plaque rupture may also occur in nonischemic settings and is not obligatorily complicated by thrombosis. furthermore, plaque rupture is not the only thrombus substrate in acute ischemic syndromes: superficia ... | 1999 | 10426860 |
| k-ras mutations in gastric stump carcinomas and in carcinomas from the non-operated stomach. | partial gastrectomy is a well-established pre-malignant condition. it is postulated that in the gastric stump an accelerated neoplastic process takes place, similar to that of (intestinal type) adenocarcinoma from the non-operated stomach. k-ras codon 12 mutation is one of the most frequent oncogenic alterations in human solid neoplasms. it is rare in conventional gastric carcinoma and has not been studied in gastric stump carcinoma. the aim of this study was to compare the prevalence of k-ras c ... | 1999 | 10430398 |
| helicobacter pylori interactions with human gastric mucin studied with a resonant mirror biosensor. | helicobacter pylori, a human pathogen colonizing the gastrointestinal tract, is first interacting with mucus glycoproteins to penetrate the gastric mucus layer and then attach to specific epithelial cell targets. an optical biosensor technique based on the resonant mirror was used to study h. pylori interactions with human gastric mucin. the mucin preparation was immobilized on the sensor surface to set up the experimental model, close to the in vivo situation. both sialylated and sulphated olig ... | 1999 | 10445316 |
| naturally acquired human immune responses against helicobacter pylori and implications for vaccine development. | | 1999 | 10446121 |
| conservation, localization and expression of hopz, a protein involved in adhesion of helicobacter pylori. | from a sarkosyl-insoluble outer membrane fraction prepared from the helicobacter pylori strain atcc 43504, 19 proteins could be sequenced n-terminally by edman degradation. oligonucleotides were deduced and used for screening of a genomic library. from the isolated genes, five code for different members of a h.pylori outer membrane protein (hop) family. among these, the hopz gene was characterized in more detail. it encodes a protein which was shown to be located at the bacterial surface by immu ... | 1999 | 10454640 |
| sulfated polysaccharide-directed recruitment of mammalian host proteins: a novel strategy in microbial pathogenesis. | fundamental to the virulence of microbial pathogens is their capacity for adaptation and survival within variable, and often hostile, environments encountered in the host. we describe a novel, extragenomic mechanism of surface modulation which may amplify the adaptive and pathogenic potential of numerous bacterial species, including staphylococcus, yersinia, and pathogenic neisseria species, as well as helicobacter pylori and streptococcus pyogenes. the mechanism involves specific bacterial recr ... | 1999 | 10456887 |
| effect of an aluminum hydroxide-magnesium hydroxide combination drug on adhesion, il-8 inducibility, and expression of hsp60 by helicobacter pylori. | co-magaldrox (maalox) is used world-wide as an antacid and as a cytoprotective agent for gastritis and peptic ulcer diseases. we examined the effects of co-magaldrox on helicobacter pylori. | 1999 | 10466876 |
| antigenic mimicry between helicobacter pylori and gastric mucosa: failure to implicate heat-shock protein hsp60 using immunoelectron microscopy. | helicobacter pylori infection induces autoantibodies that cross-react with human gastric mucosa from infected individuals. candidates for the antigens responsible for molecular mimicry causing autoreactivity include the heat-shock protein hspb (hsp60, sometimes called hsp54) or lewis x and lewis y carbohydrate antigens. | 1999 | 10469188 |
| host determinants of helicobacter pylori infection and its clinical outcome. | greater than one-half of the world's population harbors helicobacter pylori. the majority of infected individuals, however, remain asymptomatic, with only 10% to 20% developing diseases, including peptic ulcer disease, gastric cancer, and gastric mucosa-associated lymphoid tissue lymphoma. this article reviews host factors that may predispose an individual to both the acquisition of h. pylori infection and subsequent clinical outcome. individuals with specific blood group antigens and human leuk ... | 1999 | 10469193 |
| effects of mannitol and dimethylthiourea on helicobacter pylori-induced il-8 production in gastric epithelial cells. | the present study aims at investigating the effects of mannitol and dimethylthiourea, known hydroxyl radical scavengers, on lipid peroxidation as an indicative of oxidative damage, nf-kappa b activation and il-8 production by helicobacter pylori in gastric epithelial cells. a human gastric epithelial cell line, ags, treated with or without mannitol and dimethylthiourea, was incubated in the absence or the presence of h. pylori. as a result, h. pylori significantly stimulated the productions of l ... | 1999 | 10474080 |
| [are all h. pylori "bad"?]. | the infection by helicobacter pylori arises a question of great interest: should the infection be considered a disease in all cases? or, on the contrary, is the microorganism harmful in only some circumstances? the response to these questions is followed by therapeutic implications of great importance, as it is followed by the decision of administering eradication therapy to every patient or only to some individuals. in this article the arguments supporting each of the aforementioned options are ... | 1999 | 10477369 |
| [the expression of chemokines and the dynamics of inflammatory cell infiltration before and after h. pylori eradication]. | we attempted to evaluate the relationship between the expression of il-8 and rantes and the dynamics of their target cells in human gastric mucosa of h. pylori associated gastritis, including their changes after h. pylori eradication. we performed the measurement of the mucosal level of il-8 and rantes protein by elisa and immunohistochemistry. the neutrophil infiltration into the gastric mucosa was identified by the histological examination based on the updated sydney system and the measurement ... | 1999 | 10481481 |
| expression of cox-1, cox-2, and inducible nitric oxide synthase protein in human gastric antrum with helicobacter pylori infection. | for a better understanding of the regulation of prostaglandin and nitric oxide (no) synthesis in circumstances in which the gastric mucosa is inflamed, we have examined the ex vivo production of no and prostaglandin e2 and the protein expression of inducible nitric oxide synthase (inos) and 2 cyclo-oxygenase (cox) isoforms in gastric biopsies from nine helicobacter pylori-infected patients with active gastritis and six helicobacter pylori (hp)-negative patients. the results indicate a significan ... | 1999 | 10482283 |
| [helocobacter heilmannii (gastrospirillum hominis)-associated gastritis: first reported cases in hungary]. | we found a tightly coiled bacterium, helicobacter heilmannii that is distinct from helicobacter pylori in the gastric mucosal biopsy of a 55-year-old man and a 30-year-old woman, both of whom had previous history of intermittent epigastric pain. the organisms were distributed in the antral mucosa, which was endoscopically interpreted as demonstrating chronic gastritis in one case, and being normal in the other. histologically, the affected mucosa presented a mild chronic gastritis without activi ... | 1999 | 10489777 |
| review article: the pharmacology of rabeprazole. | rabeprazole sodium is a new substituted benzimidazole proton pump inhibitor with several differences compared with existing proton pump inhibitors. in vitro and animal studies have demonstrated that rabeprazole is a more potent inhibitor of h+,k(+)-atpase and acid secretion than omeprazole, and is a more rapid inhibitor of proton pumps than omeprazole, lansoprazole, or pantoprazole. this probably reflects rabeprazole's faster activation in the parietal cell canaliculus. in human studies, once-da ... | 1999 | 10491723 |
| effects of clarithromycin on the metabolism of omeprazole in relation to cyp2c19 genotype status in humans. | a triple therapy with omeprazole, amoxicillin (inn, amoxicilline), and clarithromycin is widely used for the eradication of helicobacter pylori. omeprazole and clarithromycin are metabolized by cyp2c19 and cyp3a4. this study aimed to elucidate whether clarithromycin affects the metabolism of omeprazole. | 1999 | 10511062 |
| pathobiology of helicobacter pylori infection in children. | in the pediatric population, the associations of helicobacter pylori with gastritis, gastric ulcer, duodenitis and duodenal ulcer, and with duodenal gastric surface metaplasia and disorders of the d cell- g cell axis resulting in hypergastrinemia, are well established and in many ways resemble their counterparts in adults. eradication of h pylori invariably results in the reversal of these diseases with time. there are also suggestions that gastric surface metaplasia is more extensive in childre ... | 1999 | 10519959 |
| analysis of helicobacter pylori binding site on hep-2 cells and three cell lines from human gastric carcinoma. | helicobacter pylori (h. pylori) is a pathogen responsible for chronic gastritis and peptic ulcer diseases. it colonises the gastric mucus layer and adheres to the gastric epithelial cell surface. as this adherence is the first step of infection, it is important to study the adherence mechanism. the aim of this study was to analyse the specific binding assay of h. pylori to hep-2 cells and three gastric phenotype cell lines, ags, mkn-45 and az-521. h. pylori nctc 11637 grown on agar plates was ha ... | 1999 | 10520728 |
| infection, immunity, and cancer. | a significant percentage of human cancers worldwide are associated with infections due to known viruses, including human papillomaviruses (cervical cancer and other skin cancers), human t-lymphotropic viruses (adult t-cell leukemias and lymphomas in endemic areas), hepatitis b virus (liver cancer), and epstein-barr virus (burkitt lymphoma and nasopharyngeal carcinoma). the fraction of human cancers attributable to infection may now need to be revised in light of the fact that new viral associati ... | 1999 | 10539899 |
| comparison of iron uptake in different helicobacter species. | comparison of iron uptake of four helicobacter species (helicobacter pylori, helicobacter felis, helicobacter acinonyx, and helicobacter mustelae), associated with various degrees of gastritis in their respective host, with five other species which colonize the intestinal tract of various animals (helicobacter fennelliae, helicobacter cinaedi, helicobacter muridarum, helicobacter bilis, and helicobacter hepaticus), demonstrated that the iron acquisition system differed according to the ecologica ... | 1999 | 10540911 |
| the development of functional foods: lessons from the gut. | functional foods have resulted from the gradual recognition that healthy diets result from eating nutritious foods and from the identification of the mechanisms by which foods modulate metabolism and health. after initial successes with foods that reduce blood cholesterol level, probiotic bacteria and prebiotic carbohydrates have now also demonstrated added health benefits. as ingredients become more complex, the need to stabilize such ingredients in foods become increasingly important to the su ... | 1999 | 10557163 |
| helicobacter pylori and neutrophils: sialic acid-dependent binding to various isolated glycoconjugates. | helicobacter pylori has been shown to agglutinate erythrocytes in a sialic acid-dependent manner. however, very few studies have examined relevant target cells in the human stomach. neutrophils are required for the onset of gastritis, and the inflammatory reaction may be induced on contact between bacteria and neutrophils. in the present work, glycolipids and glycoproteins were isolated from neutrophils and were studied for binding by overlay with radiolabeled bacteria on thin-layer chromatogram ... | 1999 | 10569742 |
| treatment of helicobacter pylori infection in rhesus monkeys using a novel antiadhesion compound. | helicobacter pylori can be eradicated by administration of antimicrobials, but resistant strains have emerged, and there is a need for novel therapeutic approaches against this infection. this study aimed to determine the safety and efficacy of 3'-sialyllactose sodium salt (3'sl), an oligosaccharide that occurs naturally in human and bovine milk and that can inhibit the adhesion of h. pylori to human epithelial cells in vitro. | 1999 | 10579973 |
| imunosuppression by a corticosteroid fails to exacerbate helicobacter pylori infection in a mouse model of gastric colonization. | helicobacter pylori can colonize the human stomach for prolonged periods of time, and this colonization uniformly leads to the development of chronic active gastritis. in a small percentage of individuals, gastric pathology progresses to peptic ulceration or more rarely certain gastric cancers. in addition to non-specific inflammation, specific systemic and local immunity develops in response to gastric colonization by this pathogen. however, these responses combined appear inadequate for elimin ... | 1999 | 10588046 |
| helicobacter pylori in tumor tissues of patients with advanced gastric adenocarcinoma: high prevalence but failure to detect integration. | helicobacter pylori has been known to be associated with gastric adenocarcinoma by case control studies. however, significant portion of patients with gastric carcinoma are negative for h. pylori by serological test. to further detect the presence of h. pylori infection in serum and tissue of patients with gastric adenocarcinoma, paired tissues and serum samples from 32 patients with gastric adenocarcinoma were tested. antibodies to h. pylori were tested by an enzyme linked immunosorbent assay ( ... | 1996 | 10592795 |
| endogenous nitric oxide in the regulation of gastric secretory and motor activity in humans. | studies in animals have shown that nitric oxide (no) affects gastric secretory and motor functions. however, little information is available about the involvement of this substance in the control of gastric secretory and motor activity in man. | 1999 | 10594405 |
| regression of colonic low grade b cell lymphoma of the mucosa associated lymphoid tissue type after eradication of helicobacter pylori. | lymphoma of the mucosa associated lymphoid tissue (malt) arising in the stomach has been shown to be related to helicobacter pylori infection, and total regression of gastric lymphoma after successful eradication of h pylori has consistently been reported. malt-type lymphoma at other localisations, however, has to our knowledge not been linked to h pylori, and eradication of the bacteria has not been studied for management of such lymphomas. patient/method: a 67 year old man was diagnosed with m ... | 2000 | 10601069 |
| reactivity of monoclonal antibody to hsp60 homologue of helicobacter pylori with human gastric epithelial cells and induction of il-8 from these cells by purified h. pylori hsp60. | the monoclonal antibody (mab) designated h20, which recognizes heat shock protein 60 (hsp60) of helicobacter pylori, was previously established; and the epitope recognized by the mab was shown to be species-specific. using immunohistochemical staining of six gastric biopsy specimens with the h20 mab, gastric epithelial cells of four biopsy samples stained positively. flow cytometric analysis showed that h20 mab reacted with primary human gastric epithelial (phge) cells, though the reactivities o ... | 1999 | 10616757 |
| chemotaxis and motility of helicobacter pylori in a viscous environment. | the chemotactic activity of helicobacter pylori is important for its colonization. h. pylori exhibited chemotactic responses to urea and potassium bicarbonate, which can be supplied from human gastric epithelium. the chemotactic activities of h. pylori in a fluid environment were higher on the urease-positive strain than on the isogenic urease-negative strain. in a viscous solution containing 3% polyvinylpyrrolidone, the urease-positive strain showed stimulated chemotactic activity, whereas the ... | 1999 | 10616760 |
| potential relevance of agmatine as a virulence factor of helicobacter pylori. | the polyamine agmatine is able to increase gastric acid secretion. therefore, we investigated whether helicobacter pylori is able to form and release agmatine in vitro and in the human stomach in vivo, and if so, whether a relationship exists among agmatine concentration in gastric juice, h. pylori infection, and gastroduodenal lesions. agmatine was determined by means of hplc. in the supernatant of h. pylori cultures, agmatine concentrations up to 1500 ng/ml (approximately 12 microm) were deter ... | 1999 | 10630488 |
| inhibition by 16,16-dimethyl prostaglandin e2 of tumor necrosis factor-alpha and interleukin-1beta production and messenger rna expression in human monocytes stimulated by helicobacter pylori. | we investigated the effects of 16,16-dimethyl prostaglandin e2 on the production of tumor necrosis factor-alpha and interleukin-1beta in human monocytes stimulated with helicobacter pylori. monocytes isolated from human peripheral blood were incubated for 24 hr with the extract of h. pylori diluted 1:100 to 1:100,000 by volume, a combination of the extract and 16,16-dimethyl prostaglandin e2, or a vehicle alone. the extract stimulated the production of tumor necrosis factor-alpha and interleukin ... | 1999 | 10630489 |