| [human infections by fasciola hepatica]. | | 1972 | 4554516 |
| [immunodiagnosis of human clonorchiasis by a micro-enzyme-linked immunosorbent assay]. | a micro-enzyme-linked immunosorbent assay (elisa) utilizing antigen from lyophilized clonorchis sinensis adult worms extracted with triethanolamine buffered saline (tbs), veronal buffered saline (vbs) or phosphate buffered saline (pbs) were used to test 66, 31 and 9 sera from clonorchiasis patients, uninfected normal humen and patients with other parasitic infections, respectively. four different combinations of incubation time between serum with antigen and serum with conjugate at either 37 deg ... | 1984 | 6532654 |
| [report on the first nationwide survey of the distribution of human parasites in china. 1. regional distribution of parasite species]. | a nationwide (taiwan province not included) survey of the distribution of human parasites in china during 1988-1992 was conducted under the auspices of the ministry of public health, with stratified masses randomly sampling. a total of 2,848 pilot sites in 726 counties with a population of 1,477,742 were surveyed, according to unified standard, unified diagnostic method and control quality. the overall infection rate of parasites was 62. 632%. among them, the infection rate was over 50% in 17 pr ... | 1994 | 7720194 |
| a clonorchis sinensis-specific antigen that detects active human clonorchiasis. | a clonorchis sinensis-specific antigen in excretory-secretory product of c. sinensis (cse) was assessed in human clonorchiasis by immunoblot. thirty and 7 kda antigens of cse2, one of four different batches of cses reacted strongly with infection sera from clonorchiasis patients; however, the antigens reacted weakly with 6-month post-treatment sera from praziquantel-cured cases, but were still highly detected by the sera from praziquantel-failed patients, indicating that the 30 and 7 kda antigen ... | 1998 | 9529862 |
| current status of human parasitic infections in taiwan. | the eradication of the 2 mosquito-borne parasitic diseases, malaria and lymphatic filariasis, is one of the greatest achievements of the parasite control campaigns in taiwan. most of the soil-transmitted nematode infections, with the exception of pinworm infection, are currently well controlled and limited to some aboriginal areas. food-borne parasitic zoonosis such as infections with angiostrongylus cantonensis, clonorchis sinensis, and taenia saginata asiatica are not rare, but the former is s ... | 2001 | 11605804 |
| characterization and large-scale expression of the recombinant cysteine proteinase from adult clonorchis sinensis. | cysteine proteinases play important roles in the pathogenesis of several parasitic infections and have been proposed as targets for the structure-based approach of drug design. as the first step toward applying this strategy to design inhibitors as antiparasitic agents for clonorchis sinensis, we overexpressed and characterized the 24-kda cysteine proteinase from adult worms. first, the partial cysteine proteinase gene from c. sinensis was cloned by performing reverse transcription polymerase ch ... | 2001 | 11780837 |
| studies on intestinal trematodes in korea: viii. a human case of echinostoma hortense infection. | echinostomatid eggs were found from a 21-year old man, whole native village is munkyong-gun, kyongsangnam-do during fecal examination in october 1983. the eggs were detected again one week later concommitantly with the eggs of clonorchis sinensis. he had no subjective symptoms related with these fluke infections. he was treated with praziquantel(distocide(r)) 20 mg/kg in single dose and purgated with magnesium salt. one echinostomatid fluke and 4 adults of c. sinensis were collected from the dia ... | 1983 | 12902652 |
| clonorchis sinensis infestation in man in hong kong. | | 1964 | 14137684 |
| parasitic infections in hiv-infected patients who visited seoul national university hospital during the period 1995-2003. | the prevalence of parasitic infections was investigated in human immunodeficiency virus (hiv)-infected patients (n = 105) who visited seoul national university hospital, seoul, korea, during the period from 1995 to 2003. fecal samples were collected from 67 patients for intestinal parasite examinations, and sputum or bronchoalveolar lavage samples from 60 patients for examination of pneumocystis carinii. both samples were obtained from 22 patients. thirty-three (31.4%) of the 105 were found to h ... | 2005 | 15793352 |
| determination of the minmum effective dosages of praziquantel, albendazole, and mebendazole against clonorchis sinensis infection in rats. | in order to determine the minimum effective dosages of praziquantel, albendazole, and mebendazole against clonorchis sinensis infection in sprague-dawley rats, each rat was infected with 30 metacercariae and treated with one of three drugs. the rats were killed and examined 25 days after praziquantel treatment or 11 days after albendazole or mebendazole treatment. the minimum effective dosages were a single dose of praziquantel 375 mg/kg, albendazole 150 mg/kg, and mebendazole 150 mg/kg. trials ... | 2005 | 16302447 |
| [studies on the mineral contents of some trematodes] | the mineral contents of the adult clonorchis sinensis from rabbits and human were measured, and its qualitative and semi-quantitative analyses were studied with 3.4 meter ebert-grating spectrograph, and its quantitative analyses were studied with beckman du spectrophotometer. the adult clonorchis sinensis used in this study were divided into two groups, the first group was collected from the bile passage of the man (c. sinensis from man), the second group was collected from the bile passage of t ... | 1965 | 12913579 |
| survival of adult clonorchis sinensis in the biliary passages of dead human bodies. | adult clonorchis sinensis were collected from the biliary passage of nine cases of medico-legal autopsies. the worms were washed repeatedly with tap water and rinsed twice with physiological saline. incubation took place in tyrode's medium in order to recover live worms. the number of worms surviving in the medium was determined. the rate of surviving worms showed an unexpectedly high percentage (22.8%) in those cases autopsied 2 days after death. the surviving worm burden dropped sharply to 6.3 ... | 1972 | 12913501 |
| [an epidemiological study on zoonoses in korea] | helminthic zoonoses are of significant importance to the public health and to the socioeconomic consequences especially in lost-meat, animal products and animal labor. for past several decades in korea, it has been recognized that endoparasitic infections among domestic animals are extremely common and many of the helminth parasites of man have been acquired from, or are shared with domestic animals. however, there was no survey of a nationwide scale on the prevalence of animal helminthiasis; an ... | 1981 | 12902720 |
| [a study on the intestinal helminths of the patients in a leprosarium in korea] | a total of 2,026 leprosy patients of the national sorokdo hospital was examined their intestinal parasites by cellophane thick smear method in january 1983. the egg positive cases of taenia spp. were treated with bithionol and the segments of taenia were collected for species identification. the results were as follows: 1. total egg positive rate of any kind helminth was 78.2 % and cumulative total was 85.2 %. the egg positive rate for each helminth was as follow; taenia spp. 3.4 %, ascaris lumb ... | 1983 | 12902675 |
| paleoparasitological report on the stool from a medieval child mummy in yangju, korea. | previous studies have successfully shown evidence for parasitic infections in human remains from various archaeological sites. however, in the case of korea, since there have been very few paleoparasitological reports published, pre-20th century parasitic infection patterns remain obscure. therefore, in order to partly fill this gap, we are reporting on a case of paleoparasitic infection from the feces of a 15th century child mummy from yangju, korea. in the course of the present study, we found ... | 2007 | 17626351 |
| studies on intestinal trematodes in korea xi. two cases of human infection by heterophyes heterophyes nocens. | two human cases of heterophyes heterophyes nocens infection were proved by identifying adult worms after treatment with bithionol or praziquantel in 1983 in korea. they are 37 (case 1) and 24-year old (case 2) males whose residence or native village is a southern coastal area in kohung-gun, chollanam-do. the case 1 had the gastrointestinal symptoms such as epigastric pain and indigestion, and the case 2 heart problems such as arrhythmia and ventricular premature beat in ekg and digestive symptom ... | 1984 | 12891029 |
| oral administration of a bacillus subtilis spore-based vaccine expressing clonorchis sinensis tegumental protein 22.3 kda confers protection against clonorchis sinensis. | human clonorchiasis, caused by the infection of clonorchis sinensis, is endemic in china. in this study, a full-length cdna sequence encoding c. sinensis tegumental protein 22.3 kda (cstp22.3) was identified from adult cdna library. recombinant cstp22.3 was expressed and purified from escherichia coli bl21. cstp22.3 was immunolocalized at the tegument of adult c. sinensis by using anti-recombinant cstp22.3 sera. elisa was performed using rcstp22.3 protein to investigate the igg response to cstp2 ... | 2008 | 18329763 |
| chinese liver flukes in latrine sediments from wong nim's property, san bernardino, california: archaeoparasitology of the caltrans district headquarters. | parasitological analysis of 5 sediment samples from san bernardino, california latrine deposits spanning the time period from about 1880 to the 1930s are presented. two sediment samples are from a latrine used by european-americans. three sediment samples are from latrines used by chinese-americans on the property of wong nim, an important member of the chinese community. two of the chinese latrines were positive for human parasites. the human parasites encountered include the human whipworm (tr ... | 2008 | 18372657 |
| update on hepatobiliary flukes: fascioliasis, opisthorchiasis and clonorchiasis. | hepatobiliary flukes--fasciola, opisthorchis, clonorchis- are a major public health problem in east asia, east europe, africa and latin america. the present review focuses on current knowledge of clinical, diagnostic and treatment aspects caused by hepatobiliary flukes that can be applied to current protocols in endemic areas. | 2008 | 18725803 |
| application of signaling protein 14-3-3 and 26 kda glutathione-s-transferase to serological diagnosis of schistosomiasis japonica. | schistosomiasis japonica is currently one of the most serious parasitic diseases and over 670000 people are infected in china by the end of 2006. in order to establish an effective diagnostic method, the gene coding for sj14-3-3 and sj26kda gst were cloned and expressed separately in escherichia coli as fusion protein with his-tag. the rsj14-3-3 and 26kda rsjgst were combinedly used as antigens for enzyme-linked immunosorbent assays (elisa) to diagnose acute and chronic s. japonica. our results ... | 2009 | 19560432 |
| a histidine substitution confers metal binding affinity to a schistosoma japonicum glutathione s-transferase. | glutathione s-transferases (gsts) are multifunctional enzymes that are used as fusion tags on recombinant proteins in mammalian and escherichia coli expression systems. we recently found that the schistosoma japonicum gst (sjgst) displays weak ni(2+) ion binding affinity. glu26 and his79 were assumed to be its ni(2+) binding sites based on the structure of the 26-kda clonorchis sinensis gst. to enhance sjgst ni(2+) binding affinity, glu26 was mutated to his. sjgst-e26h was expressed and purified ... | 2010 | 20347989 |
| liver fluke-associated cholangiocarcinoma. | infection with liver flukes has been reported to be associated with bile duct malignancy. | 2002 | 12153620 |
| purification and characterization of a 7-kda protein from clonorchis sinensis adult worms. | a 7-kda protein was purified from extracts of adult clonorchis sinensis by a combination of ammonium sulfate precipitation, anion exchange chromatography, cation exchange chromatography, gel-filtration chromatography, and reversed-phase fplc. the 7-kda protein exists in the excretory-secretory products of adult c. sinensis, but not in extracts of adult paragonimus westermani. also, the 7-kda protein reacted with the sera of patients with clonorchiasis but not with paragonimiasis or normal human ... | 2002 | 12099418 |
| influencing factors for cure of clonorchiasis by praziquantel therapy: infection burden and cyp3a5 gene polymorphism. | chemotherapy of clonorchiasis with praziquantel (pzq) is effective but about 15% of treated cases have been reported uncured. the present study investigated correlation of single nucleotide polymorphisms (snps) of the cytochrome p450 gene, cyp3a5 and cure of clonorchiasis. a total of 346 egg passing residents were subjected and treated by 3 doses of 25 mg/kg pzq. reexamination recognized 33 (9.5%) uncured and 313 cured. numbers of eggs per gram of feces (epgs) before treatment were significantly ... | 2011 | 21461268 |
| molecular cloning and characterization of an antigenic protein with a repeating region from clonorchis sinensis. | in the course of immunoscreening of clonorchis sinensis cdna library, a cdna csrp12 containing a tandem repeat was isolated. the cdna csrp12 encodes two putative peptides of open reading frames (orfs) 1 and 2 (csrp12-1 and -2). the repetitive region is composed of 15 repeats of 10 amino acids. of the two putative peptides, csrp12-1 was proline-rich and found to have homologues in several organisms. recombinant proteins of the putative peptides were bacterially produced and purified by an affinit ... | 2001 | 11301591 |
| isolation of liver oval cells from hamsters treated with diethylnitrosamine and 2-acetyl aminofluorene. | recently, cholangiocellular carcinoma (ccc) was successfully induced in the hamster by infecting with clonorchis sinensis following hepatocarcinogen treatment and has been proposed as a suitable model to study the pathogenesis of human ccc. in this hamster model, oval cells are suggested to be cells of origin of ccc. more direct analysis of histogenesis of ccc would become possible if large numbers of highly purified oval cells of hamster origin are obtained. in this study, we describe successfu ... | 2000 | 10770596 |
| the infestation states and changing patterns of human infecting metacercariae in freshwater fish in kyongsang-do and kyonggi-do, korea. | the infestation rates and abundances of human infecting metacercariae (clonorchis sinensis, metagonismus spp., centrocestus armatus, echinostoma hortense, echinochasmus japonicus, clinostomum complanatum) in freshwater fish were investigated at the three river areas-taewhagang (river), hyongsangang (river), nakdonggang (river) -in kyongsang-do and at four streams-yonpungchon, munsanchon, kyonganchon, and konjiamchon-in kyonggi-do, korea in 1994-1995. the fish caught at taewhagang were heavily in ... | 1996 | 8925250 |
| Molecular expression and characterization of a novel protein phosphatase 2A gene from Clonorchis sinensis. | Reversible phosphorylation of proteins is a critical mechanism involved in physiological function of organisms, including Clonorchis sinensis. In the present study, One cDNA clone encoding protein phosphatase 2A (CsPP2A) was isolated from a C. sinensis adult cDNA plasmid library. The open reading frame of the novel gene contains 924 bp and encoded a putative protein of 307 amino acids. A similarity analysis showed high homology with Schistosoma japonicum (76.3%) and Homo sapiens (84.4%), respect ... | 2011 | 22167368 |
| antigenic protein fractions of metagonimus yokogawai reacting with patient sera. | antibody test is sometimes necessary for the diagnosis of acute human metagonimiasis because eggs may not be detected in stool. the antibody test (elisa) was evaluated for its significance by reacting human sera from clinically diagnosed metagonimiasis, fascioliasis, clonorchiasis and paragonimiasis with 4 crude extracts of metagonimus yokogawai (metacercariae), adults of fasciola hepatica, clonorchis sinensis and paragonimus westermani. by elisa, 10 of 11 metagonimiasis sera showed the highest ... | 1993 | 8512898 |
| intestinal parasites of cats purchased in seoul. | fecal samples of cats purchased in seoul were examined for helminth ova or protozoan oocysts from december 1987 to march 1988. out of the 41 samples, 31 (75.6%) were positive and 60 (146.3%) were cumulative positive for parasites. the followings were identified in the samples: eggs of toxocara cati, clonorchis sinensis, metagonimus sp., pharyngostomum cordatum, spirometra erinacei, taenia taeniaeformis and oocysts of isospora sp. from nine autopsied cats, larvae of anisakis simplex, adults of c. ... | 1993 | 8297895 |
| promoting role of clonorchis sinensis infection on induction of cholangiocarcinoma during two-step carcinogenesis. | chronic clonorchis sinensis (cs) infection is etiologically related to cholangiocarcinoma (chca) in human and animals. this study was carried out to clarify the role of cs infection on dimethylnitrosamine (dmn)-induced cholangiocarcinogenesis. fifteen hamsters were administered with 15 ppm dmn for 4 weeks and one week later, the hamsters were infected with 15 metacercariae of cs (dmn-->cs group). the other 15 hamsters were infected with cs and after 5 weeks they were treated with the drug, prazi ... | 1994 | 8167103 |
| [an epidemiological study on clonorchiasis sinensis in hubei province]. | an epidemiological study on clonorchiasis sinensis was conducted in 62 counties and cities in hubei province during 1987-1991, and clonorchis sinensis was identified in 52 counties and cities. human population studies showed there were patients with clonorchiasis sinensis in 29 of 31 counties and cities investigated, with infection rates of 0.1%-16.2%. there were five species in two families of freshwater spiral shells as first intermediate host of clonorchis sinensis, 31 species in seven famili ... | 1994 | 7842884 |
| parasitological examinations and immunodiagnostic advances in fluke infections. | a reliable diagnosis of fluke infections should be based on the demonstration of parasite eggs in either faeces, duodenal liquid or sputum, depending on the type of infection. such an examination permits the identification of the species, and counting eggs establishes the intensity of infection. unfortunately this examination is useful only when intensity is rather high. the diagnosis of slight infections is uncertain and can vary considerably from one sample to another. in this area of parasito ... | 1984 | 6391499 |
| therapy of fluke infections in the past. a review. | chemotherapy of trematode infections has been studied intensively in the past. the compounds so far used for the treatment of those parasitic infections were not satisfactory, however, because of their poor tolerability and/or their limited anthelmintic efficacy. since the introduction of tartar emetic in 1918, a number of antimonial as well as non-antimonial preparations have been used for the treatment of schistosomiasis. in the treatment of clonorchiasis with antimony preparations, gentian vi ... | 1984 | 6391498 |
| a clinical study of laotian refugees infected with clonorchis sinensis or opisthorchis viverrini. | twenty-six laotian refugees infected with clonorchis or opisthorchis liver flukes, and 15 uninfected controls, were assessed clinically by history, physical examination, serum 5'nucleotidase level, and serum immunoglobulin e level. ultrasound studies of the biliary tract were also done on a subset of six infected subjects. the results showed no differences between subjects and controls with respect to clinical symptoms, physical findings, 5'nucleotidase, or biliary ultrasound. mean serum ige lev ... | 1984 | 6391229 |
| ultrastructural investigations on the effects of praziquantel on human trematodes from asia: clonorchis sinensis, metagonimus yokogawai, opisthorchis viverrini, paragonimus westermani and schistosoma japonicum. | the effect of praziquantel (biltricide) on the ultrastructure of trematodes pathogenic to man in asia was investigated in: clonorchis sinensis, opisthorchis viverrini, schistosoma japonicum, metagonimus yokogawai, and paragonimus westermani. the different parasites were isolated from their respective experimental hosts and uniformly incubated for 5, 15, 30 or 60 min at 37 degrees c in medium tc 199 containing 0, 1, 10 or 100 micrograms praziquantel/ml. all parasites exposed to praziquantel were ... | 1983 | 6338885 |
| promotion of n-nitrosodimethylamine-initiated bile duct carcinogenesis in the hamster by the human liver fluke, opisthorchis viverrini. | four groups of male golden syrian hamsters were treated as follows: group i received 50 opisthorchis viverrini metacercariae followed 41 days later by a single oral dose of 1.6 mg n-nitrosodimethylamine (ndma). group ii received a single oral dose of 1.6 mg ndma followed 96 h later by 50 o. viverrini metacercariae, group iii received a single oral dose of 1.6 mg ndma only and group iv 50 o. viverrini metacercariae only. mortality was highest in group i and ii animals receiving both ndma and para ... | 1983 | 6307539 |
| schistosoma mansoni cationic egg antigens (cef6): immunoserology with oxamniquine-treated patients and involvement of cef6 in the circumoval precipitin reaction. | the serologic activity of a cationic fraction (denoted cef6) of schistosoma mansoni soluble egg antigen was compared in an elisa with that of the unpurified soluble egg antigen for the ability to detect human infections and for the prediction of chemotherapeutic success in patients followed up to 5 years post-treatment with oxamniquine. the cationic fraction correctly identified 100% of 20 patients as infected with s. mansoni; moreover, 50% (10 of 20) seroconverted to negative by 2 years post-tr ... | 1988 | 3152779 |
| [human clonorchiasis in new endemic areas of taiwan, an epidemiological survey]. | | 1988 | 3068371 |
| multi-dot enzyme-linked immunosorbent assay for serodiagnosis of trematodiasis. | a nitrocellulose membrane strip dotted with crude antigens of trematodes, such as paragonimus miyazakii, p. westermani, fasciola sp., clonorchis sinensis and schistosoma japonicum was applied for serodiagnosis of trematodiasis. with peroxidase conjugated anti-human igg and 4-chloro-1-naphthol as a substrate, antibodies in trematodiasis patient sera were clearly detected on the strip as bluish purple spots. densities of the spots correlated well with values obtained by standard micro-elisa. cross ... | 1990 | 2075490 |
| [a comparative trial of levo-praziquantel and praziquantel in the treatment of human clonorchis sinensis infection]. | seventy patients with clonorchis sinensis infection were divided randomly into two groups and treated with levo-praziquantel and praziquantel in the same dosage of 12.5 mg/kg twice a day for two consecutive days (total dosage of 75 mg/kg). follow-up stool examination three and six months after treatment showed that the egg negative conversion rates with levo-praziquantel group were 92.86% and 92.59%, while those of praziquantel group were 58.62% and 53.57% respectively. the results showed that l ... | 1992 | 1425004 |
| pcr diagnosis of infections with different species of opisthorchiidae using a rapid clean-up procedure for stool samples and specific primers. | infections with the opisthorchiid liver flukes clonorchis sinensis, opisthorchis viverrini, and opisthorchis felineus cause serious health problems in endemic areas of southeast asia and countries of the former soviet union. chronic infections--even with low worm burdens--may lead to the development of fatal cholangiocarcinoma and related symptoms. a more sensitive diagnosis is needed since the tiny eggs of the worms are often not seen in microscopic examinations of stool samples, especially in ... | 2007 | 17061114 |
| infection status of pond smelts, hypomesus olidus, and other freshwater fishes with trematode metacercariae in 6 large lakes. | in order to determine the current infection status of pond smelts, hypomesus olidus, and other freshwater fishes with trematode metacercariae, a total of 4,861 pond smelts and 18 other freshwater fishes, collected from the soyang and uiam lakes in gangwon-do, the unam lake in jeollabuk-do, the jangseong lake in jeollanam-do, the uirim-ji (lake) in chungcheongbuk-do, and the andong lake in gyeongsangbuk-do, were individually digested with 1% pepsin-hcl and examined under a dissecting microscope. ... | 2006 | 16969063 |
| evidence of a species complex within the food-borne trematode opisthorchis viverrini and possible co-evolution with their first intermediate hosts. | the food-borne trematodes, opisthorchis viverrini, o. felineus and clonorchis sinensis, have long been recognized as the cause of major human health problems, with an estimated 40 million infected persons. of the three species of liver fluke, only o. viverrini is classified as a type 1 carcinogen because of its role as an initiator of chronic inflammation and the subsequent development of cholangiocarcinoma. at present, there are no techniques for the early diagnosis of cholangiocarcinoma and it ... | 2007 | 17275001 |
| clonorchis sinensis: expression, characterization, immunolocalization and serological reactivity of one excretory/secretory antigen-lpap homologue. | from a clonorchis sinensis adult cdna plasmid library, a cdna clone encoding a novel lysophosphatidic acid phosphatase (lpap) homologue was isolated. the predicted molecular weight of putative protein was 48.8 kda and the deduced amino acid sequence had 45%, 32%, and 29% identity with lpap of schistosoma japonicum, danio rerio, and homo sapiens, respectively. prediction of signal peptide and western blot analysis indicated that the cslpap homologue was an excretory-secretory antigen (es antigen) ... | 2007 | 17507009 |
| gene discovery for the carcinogenic human liver fluke, opisthorchis viverrini. | cholangiocarcinoma (cca)--cancer of the bile ducts--is associated with chronic infection with the liver fluke, opisthorchis viverrini. despite being the only eukaryote that is designated as a 'class i carcinogen' by the international agency for research on cancer, little is known about its genome. | 2007 | 17587442 |
| biliary parasitic diseases including clonorchiasis, opisthorchiasis and fascioliasis. | parasitic infection of the biliary tree is caused by liver flukes, namely clonorchis sinensis and opisthorchis viverrini. these flukes reside in the peripheral small bile ducts of the liver and produce chronic inflammation of the bile duct, bile duct dilatation, mechanical obstruction, and bile duct wall thickening. on imaging, peripheral small intrahepatic bile ducts are dilated, but the large bile ducts and extrahepatic bile ducts are not dilated or slightly dilated. there is no visible caused ... | 2008 | 17934771 |
| monoclonal antibodies against excretory/secretory antigens of angiostrongylus cantonensis. | in the present study, four murine monoclonal antibodies (mabs) were generated against the excretory/secretory (es) products of angiostrongylus cantonensis adult worms; two represented igg1 and two represented igm mabs, and they were designated 12d5, 15f8, 21b7 and 14g10, respectively. immunoblotting revealed that all of the mabs predominantly recognized a 98 kda antigen in the es products of a. cantonensis adult worms, and no cross reactions were found with the whole worm antigens of some other ... | 2010 | 21050048 |
| molecular cloning and characterization of a novel ras-related protein (rap2) from clonorchis sinensis. | ras are key components of diverse signal transduction pathways and play important roles in growth and development. to know about growth regulation in clonorchis sinensis, we have identified a full-length sequence encoding a ras-related protein (rap2) from our adult cdna library. the open reading frame contains 561 bp encoding 186 amino acids. the hypothetical amino acid sequence shared high identities with rap2 proteins from schistosoma japonicum and homo sapiens. conserved domains of small guan ... | 2011 | 21061020 |
| clonorchis sinensis infection is positively associated with atopy in endemic area. | epidemiologic studies have suggested that helminth infections play a protective role against allergy; this inverse association, however, has not been consistent. clonorchis sinensis, the liver fluke of human, is prevalent in the far east. the association between c. sinensis infection and allergy has not yet been reported. | 2011 | 21488996 |
| a specific pcr assay for the diagnosis of clonorchis sinensis infection in humans, cats and fishes. | clonorchiasis caused by clonorchis sinensis is a fish-borne parasitic disease which is endemic in a number of countries. using the sequences of the internal transcribed spacers (its-1 and its-2) of nuclear ribosomal dna (rdna) of c. sinensis as genetic markers, a pair of c. sinensis-specific primers was designed and used to establish a specific pcr assay for the diagnosis of c. sinensis infection in humans, cats and fish. this approach allowed the specific identification of c. sinensis after opt ... | 2011 | 21777693 |
| development of a taqman based real-time pcr assay for detection of clonorchis sinensis dna in human stool samples and fishes. | clonorchiasis caused by the oriental liver fluke clonorchis sinensis is a fish-borne zoonosis endemic in a number of countries. this article describes the development of a taqman based real-time pcr assay for detection of c. sinensis dna in human feces and in fishes. primers targeting the first internal transcribed spacer (its-1) sequence of the fluke were highly specific for c. sinensis, as evidenced by the negative amplification of closely related trematodes in the test with the exception of o ... | 2011 | 21729765 |
| Human infections of fish-borne trematodes in Vietnam: prevalence and molecular specific identification at an endemic commune in Nam Dinh province. | The prevalence of fish-borne trematodes in humans and their molecular identification was investigated in the Rang Dong commune of Nam Dinh province, Vietnam, between January 2009 and December 2010. A total of 405 people in this commune were interviewed on the habit of eating raw fish and all of their stool samples were collected using the Kato-Katz technique for examination of the presence of fish-borne trematodes. The worms (and eggs) were first morphologically examined, counted, described and ... | 2011 | 21959023 |