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dna characterization of the spirochete that causes lyme disease.lyme disease, a tick-borne disease long recognized in europe but only recently recognized in the united states, was shown in 1982-1983 to be caused by a spirochete, the lyme disease spirochete. whether one or more species of the spirochete exists is unknown, as is its taxonomic status. to answer these questions, we determined (i) the dna base (guanidine-plus-cytosine) content for five strains; (ii) the dna relatedness of 10 strains from europe or the united states (isolated from ticks, humans, a ...19846490812
adherence of borrelia burgdorferi and borrelia hermsii to mammalian cells in vitro.this study investigated the ability of borrelia burgdorferi and borrelia hermsii to attach the surface of several types of in vitro-cultured mammalian cells. borreliae showed different adhesion efficiencies depending on cell type and temperature. temperatures both lower and higher than 33 degrees and 37 degrees c respectively, decreased the adhesion of borreliae which preferentially adhere to human fibroblast-like cells. the adhesion process, mediated by structures exposed onto the surface of th ...19938469170
first isolation and cultivation of borrelia burgdorferi sensu lato from missouri.five borrelia burgdorferi sensu lato isolates from missouri are described. this represents the first report and characterization of such isolates from that state. the isolates were obtained from either ixodes dentatus or amblyomma americanum ticks that had been feeding on cottontail rabbits (sylvilagus floridanus) from a farm in bollinger county, mo., where a human case of lyme disease had been reported. all isolates were screened immunologically by indirect immunofluorescence by using monoclona ...19989431909
a novel splice variant of human gene gdpd1 is mainly expressed in human ovary and small intestine.glycerophosphodiester phosphodiesterase (gdpd) is a glycerol metabolizing enzyme that has been previously identified in escherichia coli, haemophilus influenzae, bacillus subtilis and borrelia hermsii. it has been also reported that there is putative protein containing gdpd domain in mus musculus, but not in homo sapiens. smart analysis showed that our predicted protein and three others of the same gene also comprise this domain. because the gene has not been named, we termed it gdpd1 and accord ...200314612981
comparative cryo-electron tomography of pathogenic lyme disease spirochetes.spirochetes of the borrelia burgdorferi sensu lato group, the causative agents of lyme borreliosis, exhibit a complex biology evolved in its zoonotic cycle. cryo-electron tomography was used to investigate structural features of three species, b. burgdorferi, b. garinii and b. afzelii, known to cause different clinical manifestations in humans. all three organisms revealed an overall similar architecture and showed different numbers of periplasmic flagellar filaments, polar periplasmic void regi ...200919210619
identification of an antiparallel coiled-coil/loop domain required for ligand binding by the borrelia hermsii fhba protein: additional evidence for the role of fhba in the host-pathogen interaction.borrelia hermsii, an etiological agent of tick-borne relapsing fever in north america, binds host-derived serum proteins including factor h (fh), plasminogen, and an unidentified 60-kda protein via its fhba protein. two distinct phylogenetic types of fhba have been delineated (fhba1 and fhba2). these orthologs share a conserved c-terminal domain that contains two alpha helices with a high predictive probability of coiled-coil formation that are separated by a 14-amino-acid loop domain. through s ...200818299341
spirochete-platelet attachment and thrombocytopenia in murine relapsing fever borreliosis.thrombocytopenia is common in persons infected with relapsing fever borreliae. we previously showed that the relapsing fever spirochete borrelia hermsii binds to and activates human platelets in vitro and that, after platelet activation, high-level spirochete-platelet attachment is mediated by integrin alpha iib beta 3, a receptor that requires platelet activation for full function. here we established that b hermsii infection of the mouse results in severe thrombocytopenia and a functional defe ...200312855586
opsonic requirements for phagocytosis of borrelia hermsii by human polymorphonuclear leukocytes.opsonic requirements for phagocytosis by human polymorphonuclear leukocytes (pmnls) of a laboratory strain of borrelia hermsii were examined. intracellular localization of spirochetes was confirmed by electron microscopy and cinephotomicroscopy. phagocytosis of 3h-labeled spirochetes was increased with higher concentrations of pooled human serum or greater ratios of spirochetes to pmnls and was unaffected by diminished classical complement pathway activity. immune rabbit serum markedly increased ...19827061882
tick-borne relapsing fever in british columbia, canada: first isolation of borrelia hermsii.the spirochete that causes tick-borne relapsing fever, borrelia hermsii, was isolated in pure culture during 1995 and 1996 from three acutely ill human patients infected in southern british columbia, canada. the geographic area of exposure is a known focus of this disease dating back to 1930 when the first case was recognized in a human. analyses of plasmid dna, protein profiles, and reactivity with a species-specific monoclonal antibody identified the new isolates of spirochetes as b. hermsii, ...19989817862
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