| isolation of a spermatozoa motility inhibiting factor from chicken seminal plasma with antibacterial property. | a 78-kda spermatozoa motility inhibiting factor (smif) was purified from chicken (gallus domesticus) seminal plasma by anion exchange (de-53) followed by affinity chromatography on concanavalin a-sepharose. the factor is thermostable and inhibited the spermatozoa motility in a dose dependent manner. in addition, smif inhibited the growth of gram negative bacteria, pasteurella multocida but not gram positive streptococcus equi. the factor lost its spermatozoa immobilizing property after treatment ... | 1995 | 8541320 |
| molecular studies on avian strains of pasteurella multocida in australia. | a collection of 45 strains of pasteurella multocida was assembled. the strains had been isolated from cases of fowl cholera in eastern australia over 8 years, and included mainly type a strains. all the strains were examined for plasmids and resistance to 10 antimicrobial agents and most of the strains were examined for restriction fragment length polymorphism. nine strains were assayed for pathogenicity for mice. twenty strains yielded no plasmid. seven contained a single plasmid of 1.3 kbp and ... | 1995 | 8545974 |
| stimulation of avian respiratory phagocytes by pasteurella multocida: effects of the route of exposure, bacterial dosage and strain, and the age of chickens. | the effects of the route of exposure (intratracheal [it], drinking water [dw] and aerosolization [as]), the age of chickens, and the dose of two vaccine strains of pasteurella multocida (cu and m-9) on the number, phagocytic proportion and capacity of macrophages, granulocytes and lymphocytes (collectively avian respiratory phagocytes [arps]) were analyzed. administration of p. multocida via the dw even at very high dose failed to stimulate arps. in contrast, administration of both strains of p. ... | 1995 | 8560737 |
| identification of pasteurella multocida tryptophan synthase beta-subunit by antisera against strain p1059. | pasteurella multocida strain p1059 is a highly virulent bacterium which causes fowl cholera in turkeys and chickens. a genomic library of p. multocida p1059 dna was constructed using puc19, expressed in escherichia coli dh5 alpha, and screened with chicken antisera generated against p. multocida p1059. twelve out of the 4100 clones screened were immunoreactive. plasmids isolated from these twelve clones were transformed into e. coli csr603 for maxicell analysis. five proteins, with molecular mas ... | 1996 | 8581158 |
| pasteurella multocida toxin is a mitogen for bone cells in primary culture. | the effect of recombinant pasteurella multocida toxin (pmt) on primary cultures of embryonic chick bone-derived osteoblastic cells was investigated. it was found that pmt was a potent mitogen for primary derived chicken osteoblasts. the toxin stimulated dna synthesis and cell proliferation in quiescent osteoblasts at the first passage and accelerated cell growth in subconfluent cultures. cell viability was not affected by pmt, even at relatively high concentrations. osteoblast numbers increased ... | 1996 | 8641807 |
| phenotypic characterisation of pasteurella multocida isolates from australian poultry. | a phenotypic characterisation of 110 isolates of bacteria previously identified as pasteurella multocida was performed. reference strains of many of the currently recognised species within the genus pasteurella were included in the study. all the isolates had been obtained from australian poultry-67 from chickens, 42 from turkeys and one from a duck. ten different biochemical biovars were recognised amongst the isolates. four of these biovars, representing 91 isolates, were identified as p. mult ... | 1995 | 8748543 |
| influence of chondroitinase on indirect hemagglutination titers and phagocytosis of pasteurella multocida serogroups a, d and f. | capsules of pasteurella multocida serogroups a, d and f contain mucopolysaccharides which block antigenic determinants and prevent phagocytosis. in this study, capsules of serogroup a, d and f strains of p. multocida were depolymerized by enzyme treatment. capsule depolymerization of serogroup d and f strains with chondroitinase increased indirect hemagglutination (iha) test titers and enhanced phagocytosis by swine neutrophils. capsule depolymerization of serogroup a strains with hyaluronidase ... | 1995 | 8748544 |
| antimicrobial activity of chicken and turkey heterophil peptides chp1, chp2, thp1, and thp3. | four avian heterophil antimicrobial cationic peptides (chicken heterophil peptides 1 and 2, and turkey heterophil peptides 1 and 3) were evaluated for in vitro microbicidal activity against selected avian pathogens and human pathogens which are harbored by birds. at concentrations of 16-2 micrograms/ml, all four avian peptides effected a greater than 90% reduction in the survival of candida albicans, salmonella enteriditis, and campylobacter jejuni. none of the peptides, including the known anti ... | 1995 | 8748545 |
| application of a nonlinear regression function to evaluate the kinetics of antibody response to vaccines in chicken lines divergently selected for multitrait immune response. | to evaluate the kinetics of immune response to vaccines in chickens, antibody response curves were approximated to the observed antibody ratios by using a nonlinear regression function. new parameters, the curve maximum (ymax) and the time of the maximum (tmax), were calculated. the method was applied to analyze the kinetics of the serum antibody response to mycoplasma gallisepticum (mg) and pasteurella multocida (pm) vaccines in white leghorn lines selected, in replicate, for 10 generations for ... | 1997 | 9276887 |
| cloning and characterization of the major outer membrane protein gene (omph) of pasteurella multocida x-73. | the major outer membrane protein (omph) of pasteurella multocida x-73 was purified by selective extraction with detergents, followed by size exclusion chromatography. the planar lipid bilayer assay showed that omph has pore-forming function. the average single channel conductance in 1.0 m kcl was 0.62 ns. the gene (omph) encoding omph has been isolated and sequenced by construction of a genomic library and pcr techniques. the coding region of this gene is 1,059 bp long. the predicted primary pro ... | 1997 | 9401047 |
| serologic and parasitologic survey of the endangered attwater's prairie chicken. | because conservation biologists have postulated that infectious diseases may have potentiated the endangerment of the attwater's prairie chicken (tympanuchus cupido attwateri), free-living prairie chickens were surveyed from all remaining populations for helminthic endoparasites and antibody against the etiological agents of nine infectious diseases. samples from 4 of 27 adult males were positive for anti-pasteurella multocida antibody. all other serologic tests were negative (n = 19). we identi ... | 1998 | 9476235 |
| serum and egg yolk igg antibody titers from laying chickens vaccinated with pasteurella multocida. | through determining the serum and egg yolk antibody titers in immunized laying hens to pasteurella multocida regularly, the growth-decline trend of the egg yolk antibody levels was found to be similar to that of the serum antibody levels (r = 0.94), but the growth and decline of the egg yolk antibody seemed to be delayed 3-6 days compared with that of the serum antibody, and the egg yolk antibody titers were generally lower than those of the serum antibody (p < 0.01). serum and egg yolk antibody ... | 1998 | 9533099 |
| northern bobwhites as disease indicators for the endangered attwater's prairie chicken. | because of limited access to the endangered attwater's prairie chicken (tympanuchus cupido attwateri), we used a related species, the northern bobwhite (colinus virginianus), as a surrogate for disease evaluation. free-living northern bobwhites (n = 62) on the attwater prairie chicken national wildlife refuge (near eagle lake, texas, usa) were examined during spring and fall 1993 for helminthic endoparasites and specific antibodies against the infectious agents responsible for nine infectious di ... | 1998 | 9577783 |
| pasteurella multocida infection involving cranial air spaces in white leghorn chickens. | seven 18-wk-old pullets from a commercial layer flock experiencing increased mortality associated with neurologic and respiratory symptoms were submitted to the california veterinary diagnostic laboratory system at the turlock branch for necropsy. clinical signs included depression, torticollis, swollen eyelids, conjunctivitis, and sinusitis. meningoencephalitis and suppurative inflammation of the cranial air spaces were found on histopathology. the brain, sinuses, and air spaces of the cranium ... | 1998 | 9645337 |
| pasteurella multocida infection in the chicken embryo. | pasteurella multocida infection in embryonated chicken eggs was studied by chorio-allantoic membrane inoculation. strain differences were demonstrated in terms of lesion severity and time to death, especially during the first 24 h post-inoculation. a strain of low virulence gave a clear dose response but more virulent strains did not. comparable results were obtained by infecting 6-week-old chickens. the main lesions in inoculated embryos appeared as severe vascular involvement of the entire emb ... | 1998 | 9651806 |
| therapeutic efficacy of doxycycline against experimental pasteurella multocida infection in broiler chickens. | the efficacy of doxycycline was investigated in two sets of experiments. in the first experiment 40, in the second experiment 60, hence altogether 100 five-week-old ross broilers of both sexes were used. the birds were randomly allocated into groups (a and b in experiment 1; a, b and c in experiment 2) of 20 birds in each. all birds were infected intramuscularly with approx. 2 x 10(3) colony forming units of pasteurella multocida strain x-73 (serotype a:1). birds in groups a were non-medicated c ... | 1998 | 9704513 |
| intracellular accumulation, subcellular distribution, and efflux of tilmicosin in chicken phagocytes. | tilmicosin is a semi-synthetic macrolide antibiotic, currently approved for veterinary use in cattle and swine respiratory disease, and is in development for use in poultry mycoplasma air sacculitis. in order to provide an understanding of clinical efficacy, the in vitro interaction of tilmicosin with three types of chicken phagocytes (mq-ncsu macrophages, monocyte-macrophages, and heterophils) was evaluated. after incubation with radiolabeled tilmicosin, uptake was determined and expressed as t ... | 1998 | 9776059 |
| postmortem detection of acute septicemia in broilers. | septicemia is an unwholesome condition diagnosed during postmortem inspection in poultry slaughter establishments on the basis of macroscopic lesions. early identification of septicemia has important public health implications. in this study, pasteurella multocida, escherichia coli, and staphylococcus aureus septicemia were induced in broilers in order to determine if lesions of acute septicemia can be grossly detected in the visceral organs of broiler carcasses prior to the development of chang ... | 1998 | 9777145 |
| secretion of proteases from pasteurella multocida isolates. | the capability of pasteurella multocida to secrete proteases to the culture medium and their characterization were studied in five animal isolates (bovine, chicken, sheep, and two from pig). all the isolates produced proteases in a wide range of molecular mass. it is suggested that they are neutral metalloproteases, since they were optimally active between ph 6 and 7, inhibited by chelating agents but not by other protease inhibitors, and reactivated by calcium. proteases from isolates were able ... | 1999 | 9841786 |
| sequence analysis of pasteurella multocida major outer membrane protein (omph) and application of synthetic peptides in vaccination of chickens against homologous strain challenge. | pasteurella multocida major outer membrane protein (omph) has been previously characterized as a porin. the native omph from strain x-73 (serotype 1) but not recombinant protein from escherichia coli induced homologous protection in chickens. in this study omph sequences from 15 p. multocida serotypes as well as the cu vaccine strain were compared by sequence alignment and revealed high homology, with major variations confined to two discrete regions which were correspondingly predicted as two l ... | 1999 | 10067687 |
| safety and efficacy of two live pasteurella multocida aro-a mutant vaccines in chickens. | two auxotrophic aro-a mutants of pasteurella multocida designated pmp1 (serotype 1) and pmp3 (serotype 3) were tested as vaccine candidates to protect chickens against fowl cholera. a reliable intratracheal challenge method was established that resulted in > or = 75% mortality in both specific-pathogen-free chickens and commercial broiler breeders 24 hr after challenge. dose protection studies indicated that at least 10(6) colony-forming units (cfu) of pmp1 and 10(8) cfu of pmp3 were required to ... | 1999 | 10216763 |
| effects of four antigenic fractions of pasteurella multocida serotype a on phagocytosis of chicken peripheral blood leukocytes. | the effects of four antigenic fractions of pasteurella multocida serotype a isolated from a duck in the philippines on the phagocytic activity of chicken peripheral blood leukocytes were studied by a flow cytometer. these fractions were the lipopolysaccharide-protein complex (lps), crude capsular antigen (cca), ribosomal fraction (rs) and outer cell layer (ocl). among these four antigens, only cca but not lps rs and ocl, significantly increased the phagocytic activities of mononuclear cells (mnc ... | 1999 | 10331204 |
| effects of the lipopolysaccharide-protein complex and crude capsular antigens of pasteurella multocida serotype a on antibody responses and delayed type hypersensitivity responses in the chicken. | the effects of the lipopolysaccharide-protein complex (lps) and crude capsular antigen (cca) prepared from pasteurella multocida serotype a isolated from a duck in the philippines, on antibody responses to sheep red blood cells (srbc) and brucella abortus (ba) and delayed type hypersensitivity (dth) responses to bovine serum albumin (bsa) in the chickens were studied. chickens injected subcutaneously with lps and cca at 1 and 2 weeks of age and immunized intravenously with the mixed antigens of ... | 1999 | 10379953 |
| virulence of raptor-origin pasteurella multocida in domestic chickens. | pasteurella multocida belonging to somatic serotype 1 and capsular type a has been known to cause avian cholera in domestic poultry. pasteurella multocida serotype 1 has also been isolated from raptorial birds. however, the capsular type for these raptorial isolates remains unknown. moreover, the virulence of these raptorial isolates for domestic poultry has not been determined. the objectives of this study were to determine the capsular type of raptorial p. multocida serotype 1 isolates and to ... | 1999 | 10396641 |
| restriction endonuclease analysis using hhal and hpall to discriminate among group b pasteurella multocida associated with haemorrhagic septicaemia. | the purpose of this study was to improve and standardise restriction endonuclease analysis (rea) for discriminating isolates of serogroup b pasteurella multocida associated with haemorrhagic septicaemia in wild and domestic animals and to create a reference database that can be used for epidemiological studies. two techniques for extraction and isolation of chromosomal dna were compared, a dnazol method and an enzymic lysis followed by a two-phase partition method. no differences were observed b ... | 2000 | 10628829 |
| the immunogenicity and pathogenicity of pasteurella multocida isolated from poultry in indonesia. | of 13 field isolates of pasteurella from chickens and ducks in indonesia, 10 were confirmed as p. multocida subspecies multocida, one as p. multocida subspecies gallicida and one as p. multocida subspecies septica. nine were capsular type a four were serotype 1, one was serotype 4, one was serotype 11, one was serotypes 4,12, and the remaining six were untypable. five isolates were pathogenic for mice and two were pathogenic for chickens. both a trivalent vaccine which included local field isola ... | 2000 | 10699500 |
| characteristics of a haemolytic extract from avian pasteurella multocida. | in experimental fowl cholera, the intramuscular inoculation of pasteurella multocida induces tissue damage that implies proteolytic or cytolytic activity of the bacteria. such activity could not be demonstrated by conventional in vitro tests. the treatment of p. multocida strain vp21 with tween-80 yielded an extract that lysed washed chicken red cells. extracts were active to a maximum titre of 64. haemolytic activity of the extract was neither affected by boiling nor by extremes of ph, indicati ... | 2000 | 10699501 |
| the demonstration of pasteurella multocida in the alimentary tract of chickens after experimental oral infection. | a selective medium containing polymyxin b, crystal violet, thallous acetate, bacitracin and cycloheximide in 10% sheep blood dextrose starch agar, and a modified pasteurella multocida-specific polymerase chain reaction (pcr) assay were developed for the respective isolation and detection of p. multocida from chicken alimentary tract. the selective medium and the pcr assay were highly sensitive, detecting 100 cfu from colon contents. these techniques were used to follow the localisation of an ora ... | 2000 | 10699502 |
| the virulence and protective efficacy for chickens of pasteurella multocida administered by different routes. | the relative virulence for chickens of five strains of pasteurella multocida was evaluated. twenty groups, each of ten chickens, were inoculated with a standard dose of 10(5) of each of five strains by the intramuscular (i.m.), intravenous (i.v.), intratracheal (i.tr.) or conjunctival (co) routes. the highest mortality occurred in the groups dosed i.m. and i.v., followed by i.tr. inoculation. the relative virulence of each strain did not change when inoculated by the different routes. the most v ... | 2000 | 10699503 |
| overexpression and immunogenicity of the oma87 outer membrane protein of pasteurella multocida. | the outer membrane protein of oma87 from pasteurella multocida a:1 has significant similarity to the d15 protective antigen of haemophilus influenzae (ruffolo and adler, 1996). four fragments of oma87 from a p. multocida serotype d strain were cloned into a pgex expression vector and transformed into e. coli jm105. western blot analysis revealed that convalescent chicken sera reacted with only gst-f1 fusion protein which contained amino acids 18 through to 130 of oma87 fused to the gst protein. ... | 2000 | 10699506 |
| survival of avian strains of pasteurella multocida in chicken serum. | the ability of bacteria to survive in serum is considered a likely virulence determinant in diseases where the infective bacteria become septicaemic. optimal conditions were established to test the survival of pasteurella multocida in chicken serum. serum was used at 90%, the inoculum was 10(3)-10(4)cfu in phosphate buffered saline ph 7.4. survival was measured after incubation for 2-4 h; if survival was <50% the strain was considered serum susceptible. susceptible strains were either killed or ... | 2000 | 10699511 |
| phagocytic uptake and killing of virulent and avirulent strains of pasteurella multocida of capsular serotype a by chicken macrophages. | the susceptibility of two highly virulent (vp161 and vp138) and two less virulent (vp17 and vp21) strains of pasteurella multocida to phagocytic uptake and killing by chicken macrophages was compared using in vitro phagocytosis and bactericidal assays. when compared with vp17 and vp21, particularly after they were preopsonised with specific immune serum, vp161 and vp138 were more resistant to phagocytosis by chicken macrophages. the uptake of these bacteria increased following the removal of the ... | 2000 | 10699512 |
| serum immunoglobulin g and m concentrations did not appear to be associated with resistance to pasteurella multocida in a large-bodied turkey line and a randombred control population. | serum igg and igm concentrations in a randombred control line (rbc2) of turkeys and its subline (f), selected long term for increased 16-wk bw were compared. six-week-old poults were challenged with virulent pasteurella multocida (1.2 x 10(7) bacteria per bird of serogroup a and serotype 3,4). sera were collected prior to challenge, and concentrations of igg and igm in turkey sera were quantitatively estimated with a sandwich elisa using the cross-reactive polyclonal antibodies specific for the ... | 2000 | 10735742 |
| dna fingerprinting of plasmid-containing serotype a: 3,4 pasteurella multocida isolated from cases of fowl cholera in chickens and turkeys. | the live, attenuated vaccine strains of pasteurella multocida have been hypothesized to be responsible for homologous serotype outbreaks of fowl cholera on farms that use the commercial vaccines. we have further hypothesized that the naturally occurring clemson university (cu) vaccine strain may be transformed to virulence by the acquisition of plasmid dna. to test this hypothesis, we obtained seven homologous serotype (a:3,4) p. multocida isolates, all plasmid bearing, that were cultured from f ... | 2000 | 10737663 |
| ostrich diseases. | scientific knowledge of ostrich diseases is incomplete and very fragmented, with specific details on technical aspects of diagnostic and/or screening tests completely absent in most cases. salmonella typhimurium is common in multispecies collections and causes mortality in chicks younger than three months on commercial farms, but is rarely found in chicks older than six months, or slaughter birds of twelve to fourteen months in southern africa. campylobacter jejuni and chlamydia psittaci are occ ... | 2000 | 10935285 |
| cross-protection and antigen expression by chicken embryo-grown pasteurella multocida in chickens. | the growth of pasteurella multocida strain x-73 (serotype 1) and p-1059 (serotype 3) in vivo instead of in vitro resulted in the expression of additional antigens, as revealed by sds-page profile and western blotting of the outer membrane detergent-insoluble fraction (dif-om). minor protein bands ranging from 58-138 kda were expressed by both strains; major bands ranging from 35-64 and 35-43 kda were expressed by x-73 and p-1059, respectively. antigens at 35-39 kda were dense and dominant. treat ... | 2000 | 11041997 |
| reduction of mortality in specific-pathogen-free layer chickens by a caprine serum fraction after infection with pasteurella multocida. | caprine serum was fractionated by size, and its proteinaceous material <8,000 da [caprine serum fraction immunomodulator 2 (csf-i2)] was evaluated for its ability to impart immunoresistance to specific-pathogen-free (spf) layer chickens. the spf layers were challenged with 18 to 30 cfu of pasteurella multocida x-73 (serotype 1) at 5 wk of age. a high degree of mortality was apparent 24 and 48 h later (62+/-14% and 88+/-7%, respectively). mortality observed after 48 h was minimal. noting the rapi ... | 2000 | 11055848 |
| relationships among pasteurellaceae isolated from free ranging chickens and their animal contacts as determined by quantitative phenotyping, ribotyping and rea-typing. | one hundred and forty-three pasteurella spp. strains and 10 unclassified strains obtained from free ranging poultry, dogs and cats were investigated by extended phenotypic characterization. one hundred and forty-nine of these strains were selected for further studies using ribotyping and rea-typing to evaluate the role of dogs and cats in pasteurella multocida transmission. seven and six type strains were included for comparison in phenotyping and genotyping, respectively. eleven clusters and si ... | 2001 | 11163703 |
| occurrence of pasteurella multocida and related species in village free ranging chickens and their animal contacts in tanzania. | investigation was done to determine the presence of pasteurella multocida and related species in free ranging chickens and ducks, dogs, cats and pigs in three climatic zones (cool, warm and hot) of rural morogoro, tanzania. a total of 153 isolates of p. multocida ssp. multocida and related species were obtained by direct culture on blood agar, selective medium and mouse inoculation. p. multocida ssp. multocida was isolated from 0.7% of chickens and 7% of ducks. in dogs and cats, p. multocida ssp ... | 2001 | 11163704 |
| role of capsule in the pathogenesis of fowl cholera caused by pasteurella multocida serogroup a. | we have constructed a defined acapsular mutant in pasteurella multocida x-73 (serogroup a:1) by disrupting the hexa gene through the insertion of a tetracycline resistance cassette. the genotype of the hexa::tet(m) strain was confirmed by pcr and southern hybridization, and the acapsular phenotype of this strain was confirmed by electron microscopy. the hexa::tet(m) strain was attenuated in both mice and chickens. complementation of the mutant with an intact hexab fragment restored lethality in ... | 2001 | 11254611 |
| characterization of nine pasteurella multocida isolates from avian cholera outbreaks in indonesia. | avian cholera outbreaks have been identified in indonesia in recent years. despite vaccination programs, outbreaks continue to occur. to date, there has been a lack of information on the characteristics of pasteurella multocida isolates involved in these outbreaks. hence, the objective of this study was to characterize indonesian p. multocida isolates in poultry. during 1998-99, 20 field outbreaks were reported in indonesia. nine isolates of p. multocida were recovered from these field outbreaks ... | 2001 | 11332497 |
| specific detection of pasteurella multocida in chickens with fowl cholera and in pig lung tissues using fluorescent rrna in situ hybridization. | a pasteurella multocida species-specific oligonucleotide probe, pmhyb449, targeting 16s rrna was designed and evaluated by whole-cell hybridization against 22 selected reference strains in animal tissues. it differentiated p. multocida from other bacterial species of the families pasteurellaceae and enterobacteriaceae and also from divergent species of the order cytophagales (except biovar 2 strains of pasteurella avium and pasteurella canis, which have high 16s rrna similarity to p. multocida). ... | 2001 | 11427580 |
| pathogenicity and drug susceptibility of the pasteurella anatis isolated in chickens in taiwan. | a strain of pasteurella anatis (pa) was isolated from the sinus of an adult leghorn laying chicken with sinusitis, nasal discharge, drop in egg production, and low mortality, symptoms initially thought to indicate infectious coryza. the tiny, smooth, whitish colonies were identified as pa. to compare its pathogenicity with that of commercial broilers, nine groups, 10 birds per group, of 10-day-old broilers were individually inoculated with the strain of pa, pasteurella multocida (pm), or escheri ... | 2001 | 11569739 |
| genetic diversity of pasteurella multocida fowl cholera isolates as demonstrated by ribotyping and 16s rrna and partial atpd sequence comparisons. | the genetic diversity of pasteurella multocida, the aetiological agent of fowl cholera, was investigated. the strain collection comprised 69 clinical isolates representing a wide spectrum of hosts and geographic origin. the three type strains for the subspecies of p. multocida were also included. avian isolates of p. multocida subsp. multocida and p. multocida subsp. septica did not represent separate lines by hpaii ribotyping and the two type strains of mammalian origin (porcine and cat bite) s ... | 2001 | 11577153 |
| isolation of pasteurella multocida during an outbreak of infectious septicemia in japanese quail (coturnix coturnix japonica). | in may 1994, about fifty japanese quails out of ninety being bred for experimental purposes at miyazaki university died of acute septicemia within a few days. at autopsy, there were no gross pathological lesions, however, severe bacteremia was observed in all cases. bacterial examination revealed the presence of pasteurella multocida in blood and several organs in pure culture and they were of carter's capsular type a, heddleston's type 3-4 and namioka's type o-8-9. the ld50 of bacteria in quail ... | 2001 | 11642279 |
| the effect of concurrent infections with pasteurella multocida and ascaridia galli on free range chickens. | pasteurella multocida and ascaridia galli are observed with high prevalences in free range chickens in denmark, but the impact is unknown. a study was carried out to examine the interaction between a. galli and p. multocida in chickens and the impact on production. five groups, each with 20 18-week-old lohmann brown chickens were infected. group 1 was orally infected with 1000+/-50 embryonated a. galli eggs. group 2 received 10(4) cfu p. multocida intratracheally. group 3 was infected with a. ga ... | 2002 | 11955781 |
| biofilm bacteria: formation and comparative susceptibility to antibiotics. | the calgary biofilm device (cbd) was used to form bacterial biofilms of selected veterinary gram-negative and gram-positive pathogenic bacteria from cattle, sheep, pigs, chicken, and turkeys. the minimum inhibitory concentration (mic) and minimum biofilm eradication concentration (mbec) of ampicillin, ceftiofur, cloxacillin, oxytetracycline, penicillin g, streptomycin, tetracycline, enrofloxacin, erythromycin, gentamicin, tilmicosin, and trimethoprim-sulfadoxine for gram-positive and -negative b ... | 2002 | 11989739 |
| treatment of experimentally induced pasteurella multocida infections in broilers and turkeys: comparative studies of different oral treatment regimens. | experimental fowl cholera was induced in 60 healthy 10-week-old broiler chickens and 8-week-old turkeys by intramuscular inoculation with approximately 80 colony-forming units (cfu) of pasteurella multocida x-73 strain and with approximately 70 cfu of p. multocida p-1059 strain, respectively. this method of infection proved to be useful for evaluating the efficacy of anti-microbial medication, by measuring mortality, weight gain, pathological responses and frequency of re-isolation of p. multoci ... | 2002 | 12019943 |
| serum resistance of pasteurella multocida in avian and porcine sera, and comparative virulence investigations of selected serum-sensitive and resistant strains in chickens. | growth in serum of pasteurella multocida and related species in chicken, turkey, duck and pig sera were compared, and selected serum-resistant and serum-sensitive strains were inoculated into 18-week-old layers. eighty-seven field strains of pasteurella spp. and nine reference strains representing different clones defined by restriction endonuclease analysis (rea) profiles were used in the study. serum activity was measured by changes in the optical density (od) of the serum after inoculation an ... | 2002 | 12396364 |
| genomic scale analysis of pasteurella multocida gene expression during growth within the natural chicken host. | little is known about the genomic-scale transcriptional responses of bacteria during natural infections. we used whole-genome microarray analysis to assess the transcriptional state of the gram-negative pathogen pasteurella multocida, the causative agent of fowl cholera, during infection in the natural chicken host. we compared the expression profiles of bacteria harvested from the blood of septicemic chickens experiencing late-stage fowl cholera with those from bacteria grown in rich medium. in ... | 2002 | 12438364 |
| pcr-detection of hemophilus paragallinarum, hemophilus somnus, mannheimia (pasteurella) hemolytica, mannheimia spp., pasteurella trehalosi, and pasteurella multocida. | | 2003 | 12512370 |
| evaluation of the effect of heating an oil-emulsion pasteurella multocida bacterin on tissue reaction and immunity. | killed vaccines in oil emulsions are critical components of breeder and layer vaccination programs. current vaccination sites are limited, and each has inherent problems. oil emulsion vaccines are associated with increased condemnations of spent fowl when vaccines are injected intramuscularly into the breast. in an attempt to reduce tissue reaction when injected into the breast muscle, a commercially available pasteurella multocida bacterin was heated to 41 c (100 f) for 5 hr prior to administra ... | 2003 | 12713158 |
| signature-tagged mutagenesis of pasteurella multocida identifies mutants displaying differential virulence characteristics in mice and chickens. | pasteurella multocida is the causative agent of fowl cholera in birds. signature-tagged mutagenesis (stm) was used to identify potential virulence factors in a mouse septicemia disease model and a chicken fowl cholera model. a library of p. multocida mutants was constructed with a modified tn916 and screened for attenuation in both animal models. mutants identified by the stm screening were confirmed as attenuated by competitive growth assays in both chickens and mice. of the 15 mutants identifi ... | 2003 | 12933901 |
| a comparison of virulence for mice and chickens of different colonial variants of the three serological types of pasteurella multocida. | | 1956 | 13283245 |
| studies on the pathogenicity of pasteurella multocida for mice and chickens on the basis of o-groups. | | 1964 | 14140882 |
| capsule thickness and amounts of a 39 kda capsular protein of avian pasteurella multocida type a strains correlate with their pathogenicity for chickens. | capsule thickness of avian pasteurella multocida type a strains was determined by transmission electron microscopy after labeling with polycationic ferritin and compared with their pathogenicity for chickens. the capsule thickness of p. multocida strains pm-18 and x-73 was 81.4 and 50.1 nm on average, respectively. these strains were highly virulent for chicken, whereas the less virulent strains pm-1 and pm-3 had a thin and irregular capsule, 21.0 and 29.8 nm on average, respectively. however, t ... | 2003 | 14654292 |
| a 39 kda protein mediates adhesion of avian pasteurella multocida to chicken embryo fibroblast cells. | to clarify the role of avian pasteurella multocida capsule in pathogenesis, adhesion of capsulated strains p-1059, x-73 and pm-18, and noncapsulated strains p-1059b, pm-1 and pm-3 to chicken embryo fibroblast (cef) cells was compared. number of adherent organisms of the capsulated strains to cef cells were approximately three times as much as noncapsulated strains indicating that adhesive properties were enhanced by the presence of bacterial capsule. pretreatments of the bacterial cells with hea ... | 2003 | 14654293 |
| characterization of two lipoproteins in pasteurella multocida. | an in vivo expression technology (ivet) system was previously developed and used to identify pasteurella multocida genes, which are upregulated during infection of the host. of the many genes identified, two encoded products which showed similarity to the haemophilus influenzae lipoproteins, protein d and pcp, which have been shown to stimulate heterologous immunity against infection with h. influenzae. therefore, the lipoprotein homologues in p. multocida, designated glpq and pcp, were investig ... | 2004 | 14738894 |
| studies on a pasteurella multocida chicken embryo vaccine. ii. type-specific nature of immunity elicited by a monovalent pasteurella multocida vaccine. | | 1951 | 14878125 |
| genomic-scale analysis of pasteurella multocida gene expression during growth within liver tissue of chickens with fowl cholera. | we have recently reported the gene expression profile of pasteurella multocida during growth in the blood of chickens with fowl cholera. here we report the gene expression profile of p. multocida during growth in the livers of similarly infected chickens. we compared expression profiles of bacteria harvested from the livers of infected chickens with late-stage fowl cholera with those of bacteria grown in rich medium. independent analysis of bacterial expression profiles from three individual chi ... | 2004 | 15026016 |
| characterization of a 39kda capsular protein of avian pasteurella multocida using monoclonal antibodies. | the role of a 39kda protein of avian pasteurella multocida in pathogenesis of fowl cholera was investigated using monoclonal antibodies (mabs). mabs were prepared by immunization of balb/c mice with a crude capsular extract (cce) of p. multocida strain p-1059 (serovar a:3). totally eight hybridomas producing mab were obtained. immunoblot analysis of the hybridomas revealed that all the mabs recognized a 39kda protein of cce. treatment of cce antigen with proteinase k or periodic acid indicated t ... | 2004 | 15135512 |
| a heptosyltransferase mutant of pasteurella multocida produces a truncated lipopolysaccharide structure and is attenuated in virulence. | pasteurella multocida is the causative agent of fowl cholera in birds. in a previous study using signature-tagged mutagenesis, we identified a mutant, al251, which was attenuated for virulence in mice and in the natural chicken host. sequence analysis indicated that al251 had an insertional inactivation of the gene waaq(pm), encoding a putative heptosyl transferase, required for the addition of heptose to lipopolysaccharide (lps) (m. harper, j. d. boyce, i. w. wilkie, and b. adler, infect. immun ... | 2004 | 15155650 |
| studies on a pasteurella multocida chicken embryo vaccine. i. the comparative immunizing value of broth bacterins and a chicken embryo vaccine in mice. | | 1950 | 15425748 |
| molecular epidemiology of two fowl cholera outbreaks on a free-range chicken layer farm. | two outbreaks of fowl cholera on a multiage free-range egg farm were investigated. the outbreaks occurred in 1994 and 2002. a total of 22 strains of pasteurella multocida were available for study, 11 from the 1994 outbreak and 11 from the 2002 outbreak. lesions typical of acute fowl cholera were seen in the 1994 outbreak, whereas both acute and chronic fowl cholera occurred in the 2002 outbreak. the isolates were examined in an extended phenotypic typing methodology, by a p. multocida-specific p ... | 2004 | 15460333 |
| pcr assay for rapid detection of pasteurella multocida serogroup a in morbid tissue materials from chickens with fowl cholera. | | 2004 | 15501155 |
| pasteurella multocida infection in heterophil-depleted chickens. | the present study was aimed at elucidating the role of heterophil granulocytes during the initial infection with pasteurella multocida subsp. multocida in chickens. chickens (17 and 19 wk old) were depleted of their heterophil granulocytes by 5-fluorouracil treatment. when the heterophil blood counts were significantly reduced, the birds were inoculated intratracheally with 1.8-4.3 x 10(4) colony-forming units of p. multocida. twelve, 24, or 48 hr postinoculation, the birds were euthanatized and ... | 2004 | 15529968 |
| invasion of chicken embryo fibroblast cells by avian pasteurella multocida. | invasion of chicken embryo fibroblast (cef) cells by the virulent encapsulated pasteurella multocida strains p-1059 (serovar a:3) and x-73 (serovar a:1) and an avirulent noncapsulated derivative p-1059b (serovar -:3) was investigated. the number of intracellular bacteria increased for all the strains after 2, 4 and 6 h post-inoculation to cef cells. by 6 h post-inoculation, the number of invaded bacteria of encapsulated strains was significantly higher than noncapsulated strain and reached 150- ... | 2004 | 15530739 |
| prevalent serotypes of pasteurella multocida isolated from different animal and avian species in india. | identification and estimation of the prevalence of pasteurella multocida organisms in different animal and avian species in india during november 2000 to july 2003 was carried out. out of 418 samples collected from different outbreaks suspected to be caused by p. multocida, a total of 206 bacterial cultures were identified as p. multocida on the basis of cultural, morphological and biochemical characteristics. all the 206 cultures were isolated from different domestic animal species (cattle, buf ... | 2004 | 15609866 |
| antibiotic sensitivity patterns among indian strains of avian pasteurella multocida. | an investigation was carried out to study the antibiotic sensitivity of avian strains of pasteurella multocida and to select an effective antimicrobial agent for control of avian pasteurellosis in india. a total of 123 strains of p. multocida recently isolated from different avian species (chicken, duck, turkey, quail, and goose), from different regions of india were subjected to antibiotic sensitivity tests using 20 different antibiotics. absolute resistance was observed against sulfadiazine. t ... | 2004 | 15643810 |
| vaccination against fowl cholera with acapsular pasteurella multocida a:1. | we have previously constructed an acapsular pasteurella multocida x-73 (serogroup a) mutant strain which was attenuated in virulence for chickens (chung jy, wilkie iw, boyce jd, townsend km, frost aj, ghodussi m, adler b. role of capsule in the pathogenesis of fowl cholera caused by pasteurella multocida serogroup a. infect. immun. 2001;69:2487-2492). in this study, we have assessed the ability of this acapsular strain (pba930) to induce protection against wild-type challenge in mice and the nat ... | 2005 | 15780722 |
| identification of avian strains of pasteurella multocida in india by conventional and pcr assays. | the prevalence of capsular and somatic serotypes were studied among 123 pasteurella multocida strains isolated from chickens (n=94), ducks (22), quails (4), turkeys (2) and geese (1) from different geographical regions of india. all strains exhibited similar cultural and morphological characteristics. ninety-two of the isolates belonged to serotype a:1, the most prevalent serotype, with serotypes a:3, a:1,3, d:3 and f:3 having two isolates each. only one isolate was positive for serotypes a:4 an ... | 2006 | 15994103 |
| the pnha gene of pasteurella multocida encodes a dinucleoside oligophosphate pyrophosphatase member of the nudix hydrolase superfamily. | the pnha gene of pasteurella multocida encodes pnha, which is a member of the nudix hydrolase subfamily of dinucleoside oligophosphate pyrophosphatases. pnha hydrolyzes diadenosine tetra-, penta-, and hexaphosphates with a preference for diadenosine pentaphosphate, from which it forms atp and adp. pnha requires a divalent metal cation, mg(2+) or mn(2+), and prefers an alkaline ph of 8 for optimal activity. a p. multocida strain that lacked a functional pnha gene, acp13, was constructed to furthe ... | 2005 | 16077129 |
| analysis of the pasteurella multocida outer membrane sub-proteome and its response to the in vivo environment of the natural host. | this study describes the identification of outer membrane proteins (omps) of the bacterial pathogen pasteurella multocida and an analysis of how the expression of these proteins changes during infection of the natural host. we analysed the sarcosine-insoluble membrane fractions, which are highly enriched for omps, from bacteria grown under a range of conditions. initially, the omp-containing fractions were resolved by 2-de and the proteins identified by maldi-tof ms. in addition, the omp-contain ... | 2006 | 16372271 |
| characterization of avian strains of pasteurella multocida by restriction endonuclease and amplified fragment length polymorphism. | avian strains of pasteurella multocida were typed by employing restriction endonuclease analysis (rea) and single enzyme-amplified fragment length polymorphism (aflp) to evaluate their applicability for epidemiological studies of fowl cholera outbreaks. a total of 72 strains isolated from different avian species (chicken, duck, turkey, quail and goose) belonging to various geographical regions of india were characterized. rea using two different enzymes hhai and hpaii produced 9 and 18 clusters ... | 2006 | 16427104 |
| detection of pasteurella multocida in experimentally infected embryonated chicken eggs by pcr assay. | applicability of polymerase chain reaction (pcr) assay to detect pasteurella multocida in experimentally infected embryonated chicken egg was assessed in the present study. pcr assay rapidly and specifically detected the genome of p. multocida in amniotic fluid, allantoic fluid and homogenates of infected embryo and its membranes. the sensitivity of detection was as low as 20 bacterial cells/ml of allantoic or amniotic fluids. detection of p. multocida in dead embryos by pcr was possible up to 6 ... | 2006 | 16629376 |
| fowl cholera outbreak in domestic poultry and epidemiological properties of pasteurella multocida isolate. | symptoms of fowl cholera including orofacial edema, swollen and edematous wattles and combs, and severe respiratory disorders were detected in domestic poultry in two broiler breeder farms: one located in gyeong-gi province (october, 2000) and the other in chung-cheong-nam province (march, 2001). gram-negative, bipolar staining bacillus was easily found in a direct smear. the biochemical properties of isolates were examined using a standard diagnosis method, proving that they were 99.7% similar ... | 2006 | 16820765 |
| full genome sequence and some biological properties of reticuloendotheliosis virus strain apc-566 isolated from endangered attwater's prairie chickens. | reticuloendotheliosis virus (rev) causes runting, high mortality, immunosuppression, and chronic neoplasia associated with t and/or b cell lymphomas in a variety of domestic and wild birds, including attwater's prairie chickens (apc) (tympanuchus cupido attwateri). the complete proviral sequence of a recent rev isolate from apc (rev apc-566) was determined. this virus was isolated from an apc maintained in captivity in a reproduction program intended to avoid its extinction. rev apc-566 was dete ... | 2007 | 17098316 |
| seppic vaccine adjuvants for poultry. | two inactivated antigens (newcastle and pasteurella multocida) were formulated with different adjuvants and tested in two separate experiments in poultry. oil formulations constituting water in oil (w/o) or water in oil in water (w/o/w) emulsions were assessed for antibody response, protection, local reactions, and vaccine physicochemical parameters. robust, efficacious, and safe formulations were obtained with w/o formulations whereas w/o/w was especially safe with maintained efficacy. results ... | 2006 | 17135512 |
| identification of novel immunogens in pasteurella multocida. | p. multocida is a gram-negative pathogen responsible for causing diseases in animals of economic significance to livestock industries throughout the world. current vaccines include bacterins, which provide only limited protection against homologous serotypes. therefore there is a need for more effective vaccines to control diseases caused by p. multocida. as a step towards developing vaccines against fowl cholera, a genomics based approach was applied for the identification of novel immunogens. ... | 2007 | 17233917 |
| protective immunity conferred by recombinant pasteurella multocida lipoprotein e (plpe). | the genes encoding pasteurella multocida lipoprotein e (plpe) and lipoprotein b (plpb) were cloned from p. multocida strain x-73 (serotype a:1) and expressed in escherichia coli. the protective immunity conferred by recombinant plpe (r-plpe) and plpb (r-plpb) on mice and chickens was evaluated. the results showed that mice immunized with 10microg of purified r-plpe were protected (80-100% survival rate) against challenge infection with 10 or 20 ld(50) of p. multocida strains x-73 (serotype a:1), ... | 2007 | 17449151 |
| pasteurella multocida expresses two lipopolysaccharide glycoforms simultaneously, but only a single form is required for virulence: identification of two acceptor-specific heptosyl i transferases. | lipopolysaccharide (lps) is a critical virulence determinant in pasteurella multocida and a major antigen responsible for host protective immunity. in other mucosal pathogens, variation in lps or lipooligosaccharide structure typically occurs in the outer core oligosaccharide regions due to phase variation. p. multocida elaborates a conserved oligosaccharide extension attached to two different, simultaneously expressed inner core structures, one containing a single phosphorylated 3-deoxy-d-manno ... | 2007 | 17517879 |
| decoration of pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence. | phosphocholine (pcho) is an important substituent of surface structures expressed by a number of bacterial pathogens. its role in virulence has been investigated in several species, in which it has been shown to play a role in bacterial adhesion to mucosal surfaces, in resistance to antimicrobial peptides, or in sensitivity to complement-mediated killing. the lipopolysaccharide (lps) structure of pasteurella multocida strain pm70, whose genome sequence is known, has recently been determined and ... | 2007 | 17704225 |
| quantitative resistance level (mic) of bacterial pathogens (escherichia coli, pasteurella multocida, pseudomonas aeruginosa, salmonella sp., staphylococcus aureus) isolated from chickens and turkeys: national resistance monitoring by the bvl 2004/2005. | in the study 2004/2005, the current quantitative resistance level of escherichia coli, pasteurella multocida, pseudomonas aeruginosa, salmonella sp. and staphylococcus aureus from chickens and turkeys was determined for the first time within the framework of the national resistance monitoring of the federal office of consumer protection and food safety (bvl). the objective was to implement a valid database on the basis of which the development and spread of resistance can be evaluated and monito ... | 2007 | 17939460 |
| pasteurella multocida in scavenging family chickens and ducks: carrier status, age susceptibility and transmission between species. | pasteurella multocida causes fowl cholera, a highly contagious and severe disease in chickens and water fowls. the disease is not well described in less intensive production systems, including scavenging family poultry production in developing countries. p. multocida was isolated from 25.9% of healthy-looking ducks and 6.2% of chickens from free-range family poultry farms and at slaughter slabs at market. on experimental infection with 1.2 to 2.0 x 10(8) organisms of the p. multocida type strain ... | 2008 | 18202950 |
| protection of chickens from fowl cholera by vaccination with recombinant adhesive protein of pasteurella multocida. | the recombinant adhesive protein (rcp39) of pasteurella multocida strain p-1059 (serovar a:3) was prepared and purified with a hybrid condition of affinity chromatography. the rcp39 was highly protective for chickens from fowl cholera by challenge-exposure with parental strain p-1059 or heterologous strain x-73 (serovar a:1) compared to various kind of vaccines. sixteen groups of ten chickens each were subcutaneously inoculated twice with 100, 200 or 400 microg proteins of rcp39, native cp39, na ... | 2008 | 18403068 |
| phenotypic and genotypic characters of isolates of pasteurella multocida obtained from back-yard poultry and from two outbreaks of avian cholera in avifauna in denmark. | two outbreaks of fowl cholera in the avifauna in denmark, affecting primarily eiders but also cormorants, gulls and oyster-catchers were shown to be caused by the same clone of pasteurella multocida ssp. multocida by restriction enzyme analysis (rea) and ribotyping, using the enzymes hpaii and hhai and phenotypic characterization. this observation indicated spread by migratory birds. it was shown that the outbreak clone was closely related to isolates of pasteurella multocida ssp. multocida obta ... | 1998 | 18484015 |
| studies on pasteurella gallinarum. | in two outbreaks of disease in chickens associated with pasteurella gallinarum infection swollen and inflamed wattles were symptoms. a bacteriological study was carried out of 42 p. gallinarum strains isolated from the two outbreaks and from sporadic cases in fowls. serotyping by the gel diffusion precipitin test indicated that all five strains from one outbreak and 82% of isolants from 22 sporadic cases belonged to one serotype, antigenically unrelated to pasteurella multocida. all four isolant ... | 1977 | 18770350 |
| mannan-binding lectin (mbl) in two chicken breeds and the correlation with experimental pasteurella multocida infection. | the present study is the first demonstration of an association of the genetic serum mannan-binding lectin (mbl) concentration with bacterial infections in chickens. the genetic serum mbl concentration was determined in two chicken breeds, and the association with the specific pasteurella multocida humoral immune response during an experimental infection was examined. furthermore, we examined the association of the genetic serum mbl concentration with systemic infection. the chickens with systemi ... | 2010 | 18922580 |
| characterisation and comparison of avian pasteurella multocida strains by conventional and eric-pcr assays. | sixty-one avian strains of pasteurella multocida were characterised and compared by biochemical tests, capsular pcr typing and eric-pcr. the strains were recovered from various avian species (goose, duck, muscovy duck, turkey, chicken and pheasant) and represented different geographic locations in hungary. forty-two strains (69%) were identified as p. multocida subsp. multocida and 19 strains (31%) as p. multocida subsp. septica. the strains were grouped into 7 different biovars (1, 2, 3, 4, 5, ... | 2008 | 19149098 |
| identification of novel glycosyltransferases required for assembly of the pasteurella multocida a:1 lipopolysaccharide and their involvement in virulence. | we previously determined the structure of the pasteurella multocida heddleston type 1 lipopolysaccharide (lps) molecule and characterized some of the transferases essential for lps biosynthesis. we also showed that p. multocida strains expressing truncated lps display reduced virulence. here, we have identified all of the remaining glycosyltransferases required for synthesis of the oligosaccharide extension of the p. multocida heddleston type 1 lps, including a novel alpha-1,6 glucosyltransferas ... | 2009 | 19168738 |
| investigations on the carrier rate of pasteurella multocida in healthy commercial poultry flocks and flocks affected by fowl cholera. | twenty flocks of web-footed birds (pekin and muscovy ducks and geese) and eight flocks of chickens raised under intensive management were examined for the presence of carriers of pasteurella multocida. five hundred and seventy-eight web-footed birds and 240 chickens from healthy flocks, as well as from flocks affected by fowl cholera, were investigated. a total of 135 isolates (80 from healthy flocks and 55 from flocks affected by fowl cholera) were obtained from the pharyngeal and cloacal mucos ... | 2000 | 19184799 |
| virulence of pasteurella multocida subsp. multocida isolated from outbreaks of fowl cholera in wild birds for domestic poultry and game birds. | chickens, turkeys, partridges and pheasants were experimentally infected with pasteurella multocida subsp. multocida to investigate whether outbreaks of fowl cholera in avifauna might represent a risk for organic, backyard and industrial poultry production. birds were infected intra-tracheally with a strain of p. multocida subsp. multocida (40605-1) isolated from outbreaks of fowl cholera in wild birds in denmark. p. multocida subsp. multocida strain p-1059 was included as a reference strain. th ... | 2001 | 19184870 |
| antimicrobial susceptibility and resistance of chicken escherichia coli, salmonella spp., and pasteurella multocida isolates. | escherichia coli, salmonella species, and pasteurella multocida are the major bacterial pathogens isolated from poultry. difference in susceptibility to antibiotics by microorganisms has become a major factor in drug choice and success of treatment. great concerns have been raised regarding emerging antimicrobial resistance among bacteria that may result in unpredictable antimicrobial susceptibility and failure of therapy. the primary objective of the present study was to determine the levels of ... | 2009 | 19432009 |
| mhc haplotype and susceptibility to experimental infections (salmonella enteritidis, pasteurella multocida or ascaridia galli) in a commercial and an indigenous chicken breed. | in three independent experimental infection studies, the susceptibility and course of infection of three pathogens considered of importance in most poultry production systems, ascaridia galli, salmonella enteritidis and pasteurella multocida were compared in two chicken breeds, the indigenous vietnamese ri and the commercial luong phuong. furthermore, the association of the major histocompatibility complex (mhc) with disease-related parameters was evaluated, using alleles of the lei0258 microsat ... | 2010 | 19945754 |
| experimental iron-inactivated pasteurella multocida a: 1 vaccine adjuvanted with bacterial dna is safe and protects chickens from fowl cholera. | fowl cholera is a serious problem in large and small scale poultry production. the present study describes the development and testing of an inactivated whole-cell, low-cost, safe, and effective vaccine for fowl cholera based on a previous work (vaccine 23:5590-5598). pasteurella multocida a: 1 grown in the presence of low fecl(3) concentrations, inactivated with higher concentrations of fecl(3), and adjuvanted with bacterial dna from p. multocida b: 2 containing immunostimulatory cpg motifs pro ... | 2010 | 20074684 |
| analysis of the biosynthesis genes and chemical components of the capsule of avibacterium paragallinarum. | the aim of this study was to investigate biosynthesis genes and chemical components of the capsule of avibacterium paragallinarum. the sequence of a 10-kb region containing the capsule biosynthetic locus of av. paragallinarum was determined. two reference strains, i.e., 221 (serovar a) and h18 (serovar c), together with four taiwanese field strains (all serovar c) were sequenced. the results showed that there are two genotypes (i and ii) of the capsule biosynthetic locus in av. paragallinarum, a ... | 2010 | 20362405 |
| natural selection in the chicken host identifies 3-deoxy-d-manno-octulosonic acid kinase residues essential for phosphorylation of pasteurella multocida lipopolysaccharide. | pasteurella multocida is the causative agent of a number of diseases in animals, including fowl cholera. p. multocida strains simultaneously express two lipopolysaccharide (lps) glycoforms (glycoforms a and b) that differ only in their inner core structure. glycoform a contains a single 3-deoxy-d-manno-octulosonic acid (kdo) residue that is phosphorylated by the kdo kinase, kdka, whereas glycoform b contains two unphosphorylated kdo residues. we have previously shown that p. multocida mutants la ... | 2010 | 20566690 |
| molecular epidemiology of japanese avian pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis. | molecular epidemiology analyses of the 36 clinical isolates of pasteurella multocida from various avian hosts in japan between 1976 to 2007 including 5 reference strains from the u.s.a., taiwan and indonesia were performed by employing the single-enzyme amplified fragment length polymorphism (se-aflp) comparison with the classical apai-based pulsed-field gel electrophoresis (pfge). as the results, se-aflp gave 21 profiles while pfge gave 20 profiles. the simpson's index of diversity analysis ind ... | 2010 | 20644339 |
| plasma and tissue pharmacokinetics of danofloxacin in healthy and in experimentally infected chickens with pasteurella multocida. | | 2010 | 21219355 |
| capsular serotyping of pasteurella multocida from various animal hosts - a comparison of phenotypic and genotypic methods. | one hundred and fourteen strains of pasteurella multocida were isolated from different domestic animals species (cattle, buffalo, sheep, goat, pig, rabbit, dog, cat), avian species (chicken, duck, turkey) and wild animals (deer, tiger, orang utan, marmoset). the serogroups of p. multocida were determined by both conventional capsular serotyping and a multiplex pcr assay targeting specific capsular genes. based on the conventional serotyping method, the 114 strains of p. multocida were subtyped i ... | 2011 | 21602769 |