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[the black-headed gull (larus ridibundus l.), a new host for cryptosporidium baileyi (apicomplexa: cryptosporidiidae)].black-headed full (l. ridibundus l.) represents a new host of c. baileyi current, upton and haynes, 1986. in 1991-1992 during the months of may-july six localities in three regions of the czech republic were studied by examining in total 264 not flying gull chicks (4-30 days of age), applying the methods after pavlásek (1987a, b, 1991). cryptosporidia were found in gulls in all localities and spontaneous infection during the period under study occurred in 27.5-100% of birds (tab. ii, fig. 1). th ...19938259642
molecular characterization of cryptosporidium spp. from fecal samples of birds kept in captivity in brazil.the aim of this study was to determine the prevalence of cryptosporidium species and genotypes in birds kept in captivity in brazil. a total of 966 samples from 18 families of birds was collected and stored in 5% potassium dichromate solution at 4 degrees c until processing. oocysts were purified in sheather sugar solution following extraction of genomic dna. molecular analyses were performed using nested-pcr for amplification of fragments of the 18s subunit of rrna gene and of the actin gene. a ...200919683397
[evaluation of different diagnostic techniques for oocysts of cryptosporidium baileyi in broiler chickens (gallus gallus domesticus) fecal samples].the objective of this work is to evaluate different diagnostic techniques for cryptosporidium baileyi oocysts in broiler chickens fecal samples infected naturally. so 90 samples were collected and conditioned in bottles with 10% formaline and submitted to sheater s technique in bright field. besides it, duplicate smears of the same samples were stained by safranin-methylene blue and modified ziehl-neelsen. among the staining techniques, modified ziehl-neelsen got better result in the detention o ...200820059878
biological studies and molecular characterization of a cryptosporidium isolate from ostriches (struthio camelus).there are many reports of cryptosporidial infection in ostriches, but none with molecular characterization of the isolates. a study was undertaken for the characterization of a brazilian cryptosporidium sp. ostrich isolate by using molecular phylogenetic analysis of fragments of the 18s ribosomal dna, heat-shock protein (hsp) 70 coding gene, and actin coding gene. biological studies were accomplished by the experimental inoculation of chickens via oral or intratracheal routes with fresh ostrich ...200616884009
rapid detection and quantification of cryptosporidium baileyi oocysts in feces and organs of chickens using a microscopic slide flotation method.a simple semiquantitative microscopic slide flotation (msf) method using modified sheather's sugar solution (msss) is presented for the rapid detection and quantification of cryptosporidium baileyi oocysts in the feces and mucosal and/or organ scrapings of chickens. oocyst shedding was evaluated by examination of the surface of coverslips, and the average quantitative score (0-5) recorded for 10 microscopic fields (magnification x250) is reported. the equivalence between these scores and the act ...200010726987
identification of cryptosporidium felis in a cow by morphologic and molecular methods.apicomplexan cryptosporidium parasites infect a wide range of vertebrate hosts. while some species are limited to a single host group, such as cryptosporidium baileyi, which infects chickens, other species of this genus, such as c. parvum, infect a wide range of mammalian species from mice to humans. during an investigation of cryptosporidium infection in cattle on a farm in northern poland, we identified an infection caused by c. felis, in addition to known infections with c. muris and c. parvu ...199910103236
assessment of maternal immunity to cryptosporidium baileyi in chickens.the degree of protection to cryptosporidium baileyi in the progeny of infected chickens was studied. hens at the beginning of their laying period were given orally three consecutive, large doses of c. baileyi oocysts at weekly intervals. the infection became patent after 6 days and lasted for another 6 days. increasing serum igg, and serum, bile, lachrymal and salivary iga were demonstrated from their samples. these immunoglobulins were transferred to the eggs, since high levels of maternally de ...19989823060
oocyst production and immunogenicity of cryptosporidium baileyi in chickens and mallards.two-day-old chickens and mallards were orally inoculated with one of 5 doses varying from 2 x 10(2) to 2 x 10(6) of c. baileyi oocysts per individual. generally, the more oocysts inoculated were, the longer the patent periods were, and the more oocysts shedding were. meanwhile increasing the inoculative dose, the prepatent periods were shortened except that mallards inoculated with 2 x 10(2) and 2 x 10(3) oocysts failed to produce the oocysts. the more parasites involving oocysts appeared from t ...19957735785
renal cryptosporidiosis (cryptosporidium baileyi) in specific-pathogen-free chickens experimentally coinfected with marek's disease virus.renal cryptosporidiosis was experimentally induced during a study to investigate the pathogenicity of cryptosporidium baileyi in specific-pathogen-free (spf) chickens coinfected with marek's disease virus (mdv). cryptosporidium baileyi was administered orally at 4 days of age to chickens previously infected at hatching (day 0) with the hprs 16 strain of oncogenic mdv. three control groups received mdv at hatching, c. baileyi on day 4, or placebo consisting of distilled water. renal cryptosporidi ...199910611989
potentiation of ionophorous anticoccidials with duokvin: battery trials against cryptosporidium baileyi in chickens.three battery tests, employing 444 arbor acres chickens, were conducted to study the anticryptosporidial efficacy of the polyether ionophorous anticoccidials alone and in combination with a new dihydroquinoline antioxidant, duokvin. the following efficacy percentages for the ionophores at the recommended level were established on the basis of oocyst output: lasalocid 45, monensin 37, semduramicin 29, narasin 23, and maduramicin and salinomycin 21. the efficacy of the ionophores, with the excepti ...19957472873
subclinical cryptosporidiosis of turkeys in iowa.cryptosporidium oocysts were found in the feces of 16 of 20 (80%) 17-day-old and 38 of 100 (38%) 24-day-old turkeys in a healthy commercial flock in central iowa. mean dimensions of 50 oocysts were 4.5 x 5.3 micron. in experimentally inoculated turkeys and chickens, the parasites frequently infected the ceca, colon, and cloaca, but rarely infected the small intestine or bursa of fabricius. these data differ from published descriptions of cryptosporidium baileyi in chickens and turkeys. the resul ...19883418465
[isolation and identification of cryptosporidium from various animals in korea. iii. identification of cryptosporidium baileyi from korean chicken].each of spf chicken (hi-line strain, 2-day-old males) was inoculated with 2.5 or 5 x 10(4) oocysts by stomach tube. the oocyst was the medium type of cryptosporidium previously isolated from korean chicken origin, and passed in 2-day-old spf chicken. the patterns of oocyst discharge were monitored daily, and in order to observe the ultrastructure of the developmental stages, the bursa of fabricius of the chicken was examined by transmission electron microscopy (tem) on the 12th day postinoculati ...19911804296
effect of cryptosporidium baileyi in specific pathogen free chickens vaccinated (cvi988/rispens) and challenged with hprs-16 strain of marek's.this study was performed to examine the effect of marek's disease virus (mdv) serotype 1 vaccine (cvi988/rispens) on the pathogenicity of cryptosporidium baileyi , and to determine whether c. baileyi infection could prevent the development of vaccinal marek's disease (md) immunity in specific pathogen free (spf) chickens. sixty-eight spf homozygous b13 white leghorn chickens were divided into seven groups. c. baileyi was orally administered at 5 days of age (day 4) in chickens infected with risp ...200019184860
the life cycle of cryptosporidium baileyi n. sp. (apicomplexa, cryptosporidiidae) infecting chickens.the life cycle and morphology of a previously undescribed species of cryptosporidium isolated from commercial broiler chickens is described. the prepatent period for cryptosporidium baileyi n. sp. was three days post oral inoculation (pi) of oocysts, and the patent period was days 4-24 pi for chickens inoculated at two days of age and days 4-14 for chickens inoculated at one and six months of age. during the first three days pi, most developmental stages of c. baileyi were found in the microvill ...19863735157
clinical and pathological findings in young georgia broiler chickens with oculofacial respiratory disease ("so-called swollen heads").chickens with swollen heads generally are said to have swollen head syndrome. in this retrospective study, 16 accessions of young chickens during 1992 had "swollen heads." clinical signs and lesions were accompanied by infection with multiple viruses, bacteria, and cryptosporidium baileyi. the fact that almost half of the cases of chickens with swollen heads occurred in one broiler-producing company and predictably within 14 days post-vaccination suggests that management factors might be instrum ...19947980292
a redescription of cryptosporidium galli pavlasek, 1999 (apicomplexa: cryptosporidiidae) from birds.cryptosporidium galli pavlasek, 1999, described from the feces of birds, is redescribed with additional molecular and biological data. oocysts are ellipsoidal, are passed fully sporulated, lack sporocysts, and measure 8.25 x 6.3 microm (range 8.0-8.5 x 6.2-6.4 microm) with a length-width ratio of 1.30 (n = 50). oocysts are structurally similar to those of cryptosporidium baileyi described from chickens, but in addition to being considerably larger than oocysts of c. baileyi, these oocysts infect ...200314533694
effect of broiler chicken age on susceptibility to experimentally induced cryptosporidium baileyi infection.clinical signs of respiratory tract disease were observed in chickens that were inoculated intratracheally with 1 x 10(6) oocysts of cryptosporidium baileyi at 2 or 14 days of age (10 chickens/group), but not in chickens inoculated at 28 or 42 days of age (10 chickens/group). orally inoculated chickens in all age groups (10 chickens/group) did not develop clinical signs of disease. orally and intratracheally inoculated chickens in all age groups were infected, as determined by the finding of cry ...19883178034
variations in oocyst output associated with cryptosporidium baileyi infections in chickens.groups of birds, aged 2, 16 and 30 days, were experimentally infected with 400, 20,000 or 1,000,000 oocysts of cryptosporidium baileyi. the faecal oocyst output for each group was determined by collection of faeces on days 0, 5, 7 and 13-21 post-infection (p.i.). the duration of patency of infection, as determined by oocyst excretion, declined more rapidly in the older age groups but persisted longer in birds challenged with the lower oocyst numbers.19948091620
immunosuppressive effect of cryptosporidium baileyi infection on vaccination against avian infectious bronchitis in chicks.two-day-old commercial chicks were inoculated orally with 2 x 10(6) oocysts of cryptosporidium baileyi and vaccinated with 10(3.5) eid50/head of a commercially available avian infectious bronchitis (ib) live virus vaccine at 4 and 14 days following inoculation. chicks infected with c. baileyi were shown to have an immunosuppressive effect on ib virus. it is concluded that infection with the protozoon in early life may increase their susceptibility to ib.19989755592
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