| comparative population structure analysis of campylobacter jejuni from human and poultry origin in bangladesh. | campylobacter jejuni is the most important cause of antecedent infections leading to guillain-barré syndrome (gbs) and miller fisher syndrome (mfs). the objective of the present study was to define the genetic diversity, population structure, and potential role of poultry in the transmission of campylobacter to humans in bangladesh. we determined the population structure of c. jejuni isolated from poultry (n = 66) and patients with enteritis (n = 39) or gbs (n = 10). lipooligosaccharide (los) ty ... | 2014 | 24962195 |
| the effects of temperature and innate immunity on transmission of campylobacter jejuni (campylobacterales: campylobacteraceae) between life stages of musca domestica (diptera: muscidae). | the house fly (musca domestica l.) is a well-established vector of human pathogens, including campylobacter spp., which can cause infection of broiler chicken flocks, and through contaminated broiler meat can cause outbreaks of campylobacteriosis in humans. we investigated whether campylobacter jejuni (jones) could be transferred between life stages of m. domestica (larvae-pupae-adults) and determined bacterial counts of c. jejuni at different time points after bacterial exposure. c. jejuni was ... | 2014 | 24897861 |
| campylobacter jejuni influences the expression of nutrient transporter genes in the intestine of chickens. | the gastrointestinal tract represents the first barrier against pathogens. however, the interaction of campylobacter with intestinal epithelial cells and its effects on the intestinal function of chickens are poorly studied. therefore, the goal of the present study was to characterize the effects of c. jejuni oral infection on the mrna expression of nutrient transporters in the intestine. newly hatched specific pathogen-free (spf) chickens were orally infected with c. jejuni (nctc 12744; 1 × 10( ... | 2014 | 24834798 |
| campylobacter infection has different outcomes in fast- and slow-growing broiler chickens. | campylobacter spp. are frequently carried by poultry, but they are not believed to cause significant disease in these animals. modern poultry breeds have been selected to grow rapidly under intensive conditions, but recently, consumers have moved toward purchasing birds produced in higher welfare, free-range or organic systems. birds reared in these systems tend to be a slower growing breed and are fed a different diet. birds reared in such systems are stocked at a lower density compared with th ... | 2013 | 24689180 |
| genetic diversity and antibiotic resistance profiles of campylobacter jejuni isolates from poultry and humans in turkey. | in this study, the investigation of clonal relations between human and poultry campylobacter jejuni isolates and the determination of susceptibilities of isolates to various antibiotics were aimed. a total of 200 c. jejuni isolates concurrently obtained from 100 chicken carcasses and 100 humans were genotyped by the pulsed-field gel electrophoresis (pfge) and automated repetitive extragenic palindromic pcr (rep-pcr, diversilab system) methods and were tested for their susceptibility to six antib ... | 2014 | 24667316 |
| characterization and reactivity of broiler chicken sera to selected recombinant campylobacter jejuni chemotactic proteins. | campylobacter jejuni, a gram-negative rod bacterium, is the leading causative agent of human acute bacterial gastroenteritis worldwide. consumption and handling of raw or undercooked poultry are regarded as a major source for human infection. because bacterial chemotaxis guides microorganisms to colonization and invasion in the host cells, proteins involved in chemotactic processes can be novel targets for vaccine development. in this communication, we report amplification, cloning and expressio ... | 2014 | 24609189 |
| effects of commercial marinade seasoning and a natural blend of cultured sugar and vinegar on campylobacter jejuni and salmonella typhimurium and the texture of chicken breasts. | marination using various ingredients has been widely used to improve microbial safety and quality of chicken products at retail markets. the objective of this study was to investigate the effects of commercial marinade seasoning and cultured sugar/vinegar blend on campylobacter jejuni and salmonella typhimurium populations during refrigerated storage. in addition, their effects on the texture of precooked chicken breasts during frozen and refrigerated storage was investigated. chicken breasts in ... | 2014 | 24604867 |
| campylobacter infection in chickens modulates the intestinal epithelial barrier function. | asymptomatic carriage of campylobacter jejuni is highly prevalent in chicken flocks. thus, we investigated whether chronic campylobacter carriage affects chicken intestinal functions despite the absence of clinical symptoms. an experiment was carried out in which commercial chickens were orally infected with c. jejuni (1 × 10(8) cfu/bird) at 14 days of life. changes in ion transport and barrier function were assessed by short-circuit current (i(sc)) and transepithelial ion conductance (g(t)) in ... | 2015 | 24553586 |
| the evidence for clonal spreading of quinolone resistance with a particular clonal complex of campylobacter jejuni. | campylobacter is the most prevalent cause of bacterial gastroenteritis worldwide and it represents a significant public health risk of increasing severity due to its escalating resistance to clinically important quinolone and macrolide antibiotics. as a zoonotic pathogen campylobacter is transmitted along the food chain and naturally cycles from environmental waters, feedstuff, animals and food to humans. we determined antibiotic resistance profiles, as well as multilocus sequence types and flaa ... | 2014 | 24534165 |
| reduction of microbiological risk in minced meat by a combination of natural antimicrobials. | responsibility for food safety must be taken through the entire food-production chain, to avoid consumer cross-contamination. the antimicrobial activities of an alpinia katsumadai seed extract and epigallocatechin gallate (egcg), and their combination, were evaluated against individual food-borne pathogenic strains of listeria monocytogenes, escherichia coli and campylobacter jejuni, individually and as a cocktail, in chicken-meat juice and sterile minced meat as food models, and in minced meat ... | 2014 | 24532379 |
| characterization and antigenicity of recombinant campylobacter jejuni flagellar capping protein flid. | campylobacter jejuni, a flagellated, spiral-rod, gram-negative bacterium, is the leading pathogen of human acute bacterial gastroenteritis worldwide, and chickens are regarded as a major reservoir of this micro-organism. bacterial flagella, composed of more than 35 proteins, play important roles in colonization and adhesion to the mucosal surface of chicken caeca. in this study, the flagellar capping protein, flid, encoded by the flid gene, from the campylobacter jenuni d1-39 isolate was express ... | 2014 | 24445509 |
| double-locus sequence typing using pora and peb1a for epidemiological studies of campylobacter jejuni. | campylobacter jejuni is the leading cause of foodborne bacterial gastroenteritis worldwide. bacterial typing schemes play an important role in epidemiological investigations to trace the source and route of transmission of the infectious agent by identifying outbreak and differentiating among sporadic infections. in this study, a double-locus sequence typing (dlst) scheme for c. jejuni based on concatenated partial sequences of pora and peb1a genes is proposed. the dlst scheme was validated usin ... | 2014 | 24404778 |
| screening of genes expressed in vivo during interaction between chicken and campylobacter jejuni. | chicken are considered as the most important source of human infection by campylobacter jejuni, which primarily arises from contaminated poultry meats. however, the genes expressed in vivo of the interaction between chicken and c. jejuni have not been screened. in this regard, in vivo-induced antigen technology (iviat) was applied to identify expressed genes in vivo during interaction between chicken and c. jejuni, a prevalent foodborne pathogen worldwide. chicken sera were obtained by inoculati ... | 2014 | 24225374 |
| molecular epidemiology of campylobacter jejuni human and chicken isolates from two health units. | a study was conducted over a 2-year period in the perth district and wellington-dufferin-guelph health units in ontario, with an objective of using comparative genomic fingerprinting (cgf) with a 40-gene assay (cgf40) to investigate the association between human cases of campylobacteriosis and spatially and temporally related campylobacter isolates from retail chicken. cgf results were available for isolates from 115 human cases and 718 retail chicken samples. these data were combined with cgf r ... | 2014 | 24219173 |
| multistate outbreak of campylobacter jejuni infections associated with undercooked chicken livers--northeastern united states, 2012. | in october 2012 the vermont department of health (vdh) identified three cases of laboratory-confirmed campylobacter jejuni infection in vermont residents; the isolates had indistinguishable pulsed-field gel electrophoresis (pfge) patterns. a query of pulsenet, the national molecular subtyping network for foodborne disease surveillance, led to the identification of one additional case each from new hampshire, new york, and vermont that had been reported in the preceding 6 months. an investigation ... | 2013 | 24196663 |
| campylobacteriosis in returning travellers and potential secondary transmission of exotic strains. | multilocus sequence types (sts) were determined for 232 and 737 campylobacter jejuni/coli isolates from dutch travellers and domestically acquired cases, respectively. putative risk factors for travel-related campylobacteriosis, and for domestically acquired campylobacteriosis caused by exotic sts (putatively carried by returning travellers), were investigated. travelling to asia, africa, latin america and the caribbean, and southern europe significantly increased the risk of acquiring campyloba ... | 2014 | 23962634 |
| tetrazolium reduction allows assessment of biofilm formation by campylobacter jejuni in a food matrix model. | to develop a staining method for specific detection of metabolically active (viable) cells in biofilms of the foodborne pathogen campylobacter jejuni. | 2013 | 23910098 |
| quantum dot-based immunochromatography test strip for rapid detection of campylobacter jejuni. | campylobacter jejuni is a recognized human foodborne pathogen which is one of the leading causes of human gastrointestinal enteritis worldwide. in stress conditions, c. jejuni is able to enter into a viable but non-culturable state that is difficult to diagnose. hence a rapid, sensitive, and specific method is required to monitor food and water for natural or intentional contamination by this pathogen. we report a quantum dot (qd)-based immunochromatography test strip (qdits) for rapid detection ... | 2013 | 23901473 |
| phage receptor binding protein-based magnetic enrichment method as an aid for real time pcr detection of foodborne bacteria. | we present a novel phage receptor binding protein-based magnetic separation and pre-enrichment method as an alternative to the immunomagnetic separation methods by replacing antibodies with bacteriophage receptor binding proteins (rbps). we couple the proposed rbp-based magnetic separation with real time pcr for rapid, sensitive and specific detection of campylobacter jejuni cells in artificially contaminated skim milk, milk with 2% fat and chicken broth. recovery rates, assessed by real time pc ... | 2013 | 23897488 |
| evaluation of nanoparticle-encapsulated outer membrane proteins for the control of campylobacter jejuni colonization in chickens. | numerous vaccination strategies have been evaluated to develop effective vaccines against campylobacter jejuni colonization in poultry but with limited success. the following experiments were conducted to investigate the effect of biodegradable and biocompatible poly (lactide-co-glycolide) nanoparticle (np) encapsulated outer membrane proteins (omp) of c. jejuni. chickens were vaccinated with different routes [subcutaneous (s/c) or oral] and doses (25, 125, or 250 µg) of candidate nanoparticle v ... | 2013 | 23873570 |
| a novel immunoproteomics method for identifying in vivo-induced campylobacter jejuni antigens using pre-adsorbed sera from infected patients. | campylobacter jejuni is an important food-borne and zoonotic pathogen with a worldwide distribution. humans and chickens are hosts of this pathogen. at present, there is no ideal vaccine for controlling human campylobacteriosis or the carriage of c. jejuni by chickens. bacterial in vivo-induced antigens are useful as potential vaccine candidates and biomarkers of virulence. | 2013 | 23850640 |
| distribution of nine virulence-associated genes in campylobacter jejuni and c. coli isolated from broiler feces in shiraz, southern iran. | to investigate the prevalence of nine virulence and toxin genes of campylobacter, a total of 90 campylobacter strains including 48 c. jejuni and 42 c. coli were recovered from chicken feces by cultivation methods. the isolates were identified on the basis of polymerase chain reaction (pcr) detection of 16srrna and multiplex pcr for determining two species. for confirmed strains, pcr was carried out for the presence of virulence genes using specific primers. data were analyzed by spss software, v ... | 2013 | 23789768 |
| production and evaluation of chicken egg-yolk-derived antibodies against campylobacter jejuni colonization-associated proteins. | campylobacter jejuni is one of the most important causes of foodborne gastroenteritis. chickens are considered a reservoir host of c. jejuni, and epidemiological studies have shown that contaminated chicken meat is a primary source of human infection. the objective of this study was to produce chicken egg-yolk-derived antibody (igy) against the five c. jejuni colonization-associated proteins or caps (cadf, flaa, momp, flpa, and cmec). recombinant c. jejuni caps were expressed in escherichia coli ... | 2013 | 23742296 |
| role of motab in adherence and internalization in polarized caco-2 cells and in cecal colonization of campylobacter jejuni. | campylobacter jejuni, a gram-negative motile bacterium commonly found in the chicken gastrointestinal tract, is one of the leading causes of bacterial gastroenteritis in humans worldwide. an intact and functional flagellum is important for c. jejuni virulence and colonization. to understand the role of c. jejuni motility in adherence and internalization in polarized caco-2 cells and in cecal colonization of chickens we constructed a c. jejuni nctc11168 v1 deltamotab mutant. the motab genes code ... | 2013 | 23678739 |
| [molecular analysis of fluoroquinolones and macrolides resistance in campylobacter jejuni isolates from humans, bovine and chicken meat]. | campylobacter sp.- one of the leading causes of bacterial food-borne gastrointestinal illness worldwide- is increasingly resistant to fluoroquinolone and macrolide antimicrobials, which has become a major concern for public health. | 2013 | 23677151 |
| immunity to bacterial infection in the chicken. | bacterial infections remain important to the poultry industry both in terms of animal and public health, the latter due to the importance of poultry as a source of foodborne bacterial zoonoses such as salmonella and campylobacter. as such, much focus of research to the immune response to bacterial infection has been to salmonella. in this review we will focus on how research on avian salmonellosis has developed our understanding of immunity to bacteria in the chicken from understanding the role ... | 2013 | 23648643 |
| antimicrobial resistance of campylobacter jejuni isolated from chicken carcasses in the federal district, brazil. | the aim of the present study was to perform microbiological isolation of campylobacter jejuni from chilled chicken carcasses marketed in the federal district of brazil and to subject the strains to an antibiogram. a total of 92 samples from chilled chicken carcasses were acquired, 18 of which (19.56%) tested positive for c. jejuni. a total of 16 strains were tested for susceptibility to eight antimicrobial drugs. all 16 strains were resistant to ciprofloxacin, 15 strains to nalidixic acid, strep ... | 2013 | 23575135 |
| is allicin able to reduce campylobacter jejuni colonization in broilers when added to drinking water? | reducing campylobacter shedding on the farm could result in a reduction of the number of human campylobacteriosis cases. in this study, we first investigated if allicin, allyl disulfide, and garlic oil extract were able to either prevent c. jejuni growth or kill c. jejuni in vitro. allyl disulfide and garlic oil extract reduced c. jejuni numbers in vitro below a detectable level at a concentration of 50 mg/kg (no lower concentrations were tested), whereas allicin reduced c. jejuni numbers below ... | 2013 | 23571353 |
| prevalence and risk assessment of campylobacter jejuni in chicken in china. | to understand the occurrence and distribution of campylobacter jejuni in chicken in china, assess its health risk to the chinese population, and provide recommendations for effective risk control. | 2013 | 23534464 |
| distribution of colonization and antimicrobial resistance genes in campylobacter jejuni isolated from chicken. | campylobacter jejuni is an important worldwide foodborne pathogen commonly found as a commensal organism in poultry that can reach high numbers within the gut after colonization. although information regarding some genes involved in colonization is available, little is known about their distribution in strains isolated specifically from chickens and whether there is a linkage between antimicrobial resistance (amr) and colonization genes. to assess the distribution and relevance of genes associat ... | 2013 | 23510494 |
| induction of chicken cytokine responses in vivo and in vitro by lipooligosaccharide of campylobacter jejuni hs:10. | campylobacter jejuni is a pathogen of the gastrointestinal tract of humans, but colonizes chickens for prolonged periods without causing disease. it is unclear what host and bacterial mechanisms maintain a non-inflammatory state in chickens. the present work was undertaken to characterize cytokine responses of chickens to purified lipooligosaccharide (los) of c. jejuni hs:10. chickens were injected with purified los, and expression of interleukin (il)-1β (pro-inflammatory cytokine), il-8 (pro-in ... | 2013 | 23473646 |
| effect of steam and lactic acid treatments on the survival of salmonella enteritidis and campylobacter jejuni inoculated on chicken skin. | campylobacteriosis and salmonellosis are the most frequently reported zoonotic infectious diseases. the present work evaluated the effectiveness of steam treatment at 100 °c for 8s, a 5% lactic acid treatment for 1 min and their combination for inactivating salmonella enteritidis and campylobacter jejuni inoculated on chicken skin. the impact of each treatment on the total aerobic mesophilic bacteria and the effect of rinsing after contact with lactic acid were also evaluated. residual bacteria ... | 2013 | 23454819 |
| phenotypic and genetic analyses of campylobacter jejuni lior serotype 76 isolated from chicken meat and clinical specimens. | the aim of this study was to examine the link between campylobacter jejuni isolates obtained from chicken meat (n = 7) and gastroenteritis patients (n = 744). in total, 751 isolates were subjected to lior serotyping. all the isolates from chicken meats were serotyped as lior serotype 76 (lio76). among 23 of the identified lio76 strains, 13 strains (6 from chicken meat and 7 from clinical specimens) were indistinguishable by penner serotyping, antimicrobial susceptibility testing, and pulsed-fiel ... | 2013 | 23429091 |
| continued widespread dissemination and increased poultry host fitness of campylobacter jejuni st-4526 and st-4253 in japan. | campylobacter jejuni is a major cause of foodborne gastroenteritis. we previously reported the widespread camp. jejuni sequence type (st)-4526 in japan from 2005 to 2006. this study assesses the potential for this genotype to thrive thereafter. | 2013 | 23351059 |
| use of plant-derived antimicrobials for improving the safety of poultry products. | salmonella enteritidis and campylobacter jejuni are the 2 major foodborne pathogens transmitted through poultry products. chickens are the reservoir hosts of these pathogens, with their intestinal colonization being the most significant factor causing contamination of meat and eggs. effective preslaughter strategies for reducing the colonization of birds with these pathogens are critical to improve the microbiological safety of poultry products. an antimicrobial treatment that can be applied thr ... | 2013 | 23300319 |
| colonization of chicken flocks by campylobacter jejuni in multiple farms in japan. | campylobacter jejuni was monitored in 4 chicken farms during the period 2003 to 2006 to elucidate the mechanisms of transmission. three farms (1 to 3), located at least 14 km from each other, belonged to an integrated poultry company, which also provided the farms with day-old chicks from several hatcheries as well as chicken feed. another farm (4), which belonged to a different company, was located 270 m from farm 1. a total of 206 c. jejuni isolates obtained from the 4 farms were classified in ... | 2013 | 23300304 |
| zoonotic fecal pathogens and antimicrobial resistance in county fair animals. | livestock fairs present a unique opportunity for the public to experience close contact with animals, but may also expose people to zoonotic pathogens through contact with animal feces. the goal of this study was to screen cattle, sheep, goat, chicken, rabbit and horse feces from a livestock fair in california for the potentially zoonotic pathogens escherichia coli o157:h7, salmonella, campylobacter, vibrio, cryptosporidium and giardia spp., as well as determining the level of antimicrobial resi ... | 2013 | 23260373 |
| in vivo broiler experiments to assess anti-campylobacter jejuni activity of a live enterococcus faecalis strain. | bacterial gastroenteritis caused by thermotolerant campylobacter species, mainly campylobacter jejuni, has been the most reported zoonotic disease in many developed countries in recent years. reducing campylobacter shedding on the farm could result in a reduction of the number of campylobacteriosis cases. in 2 independent broiler seeder experiments, in which broiler chickens were orally inoculated with 2 amounts of enterococcus faecalis mb 5259, we established whether a live e. faecalis strain w ... | 2013 | 23243257 |
| prevalence of the main food-borne pathogens in retail food under the national food surveillance system in japan. | the national food surveillance system in japan was formed in 1998 to monitor the contamination of retail foods with bacterial pathogens. approximately 2000-3000 samples were tested annually, and the data from food categories that had more than 400 samples collected during 1998-2008 were analysed. with regard to meat, the frequency of positive samples for salmonella in chicken for raw consumption and ground chicken was 12.7% and 33.5%, respectively. moreover, shiga toxin-producing escherichia col ... | 2013 | 23199079 |
| design and data analysis of experimental trials to test vaccine candidates against zoonotic pathogens in animals: the case of a clinical trial against campylobacter in broilers. | the development of effective vaccines against zoonotic pathogens represents a priority in public health protection programs. the design of clinical trials and appropriate data analysis of the experiments results are crucial for the assessment of vaccine effectiveness. this manuscript reviews important issues related to the assessment of the effectiveness of vaccines designed to obtain a quantitative reduction of the pathogen in animals or animal products. an effective vaccine will reduce the ris ... | 2012 | 23176651 |
| application of ozonated dry ice (aligal™ blue ice) for packaging and transport in the food industry. | dry ice is used by meat and poultry processors for temperature reduction during processing and for temperature maintenance during transportation. aligal™ blue ice (abi), which combines the antimicrobial effect of ozone (o(3)) along with the high cooling capacity of dry ice, was investigated for its effect on bacterial reduction in air, in liquid, and on food and glass surfaces. through proprietary means, o(3) was introduced to produce dry ice pellets to a concentration of 20 parts per million (p ... | 2012 | 23163945 |
| in vitro evaluation of the probiotic potential of lactobacillus salivarius smxd51. | lactobacillus salivarius smxd51 was previously isolated from the cecum of a tunisian poultry and found to produce a bacteriocin-like substance highly active against the foodborne pathogen campylobacter jejuni. the aim of this study was to examine some probiotic properties of the strain: acid and bile tolerance, capacity of adhesion, stimulation of immune defences (il-6, il-8, il-10 and β-defensin 2), and modulation of the barrier integrity. the results showed that l. salivarius smxd51 can tolera ... | 2012 | 23122647 |
| development of a feed additive to reduce caecal campylobacter jejuni in broilers at slaughter age: from in vitro to in vivo, a proof of concept. | in vitro and in vivo challenge studies were undertaken to develop an in-feed additive of microencapsulated propionic, sorbic acids and pure botanicals to control campylobacter jejuni in broilers at slaughter age. | 2013 | 23110383 |
| control of campylobacter jejuni in chicken breast meat by irradiation combined with modified atmosphere packaging including carbon monoxide. | campylobacter is one of the leading causes of human foodborne illnesses originating from meat and poultry products. cross-contamination of this organism occurs in many poultry processing plants, and can occur in the kitchens and refrigerators of consumers. therefore, new intervention strategies are needed for meat and poultry products to better protect consumers from this pathogen. vacuum or modified atmosphere packaging is a common packaging technique used by the meat and poultry industry to ex ... | 2012 | 23043819 |
| the isolation and characterization of campylobacter jejuni bacteriophages from free range and indoor poultry. | six hundred and sixty one samples - primarily fresh chicken faeces - were processed to isolate wild type campylobacter jejuni bacteriophages, via overlay agar methods using c. jejuni nctc 12662. the aims of this study were to isolate and purify bacteriophages and then test for their ability to lyse field strains of c. jejuni in vitro. of all samples processed, 130 were positive for bacteriophages. a distinct difference was observed between samples from different poultry enterprises. no bacteriop ... | 2013 | 22980913 |
| sodium metasilicate affects growth of campylobacter jejuni in fresh, boneless, uncooked chicken breast fillets stored at 4 degrees celsius for 7 days. | the objectives of this study were to determine the antimicrobial effects of sodium metasilicate (sms) treatments against campylobacter jejuni in fresh, boneless, uncooked chicken breast fillets and to ascertain the effects of sms treatments on ph. the fillets were inoculated with c. jejuni, treated with 0% sms and no inoculum (negative control), 0% sms and inoculum (positive control), 1 and 2% sms solutions, and stored at 4 ± 1°c. all samples were analyzed after 0, 1, 3, 5, and 7 d storage for c ... | 2012 | 22912470 |
| garlic derivative propyl propane thiosulfonate is effective against broiler enteropathogens in vivo. | two experiments were carried out to study the effects of dietary supplementation with the garlic (allium sativum)-derived product propyl propane thiosulfonate (pts-o) on the intestinal log(10) number of copies of enteropathogens in broiler chickens, together with their intestinal morphology and growth performance. the additive had no significant effect on feed intake at any dose assayed. in experiment 1 (1 to 21 d of age), the bw of chickens fed on 45 mg of pts-o/kg of diet was higher (p < 0.01) ... | 2012 | 22912448 |
| effect of crust freezing applied alone and in combination with ultraviolet light on the survival of campylobacter on raw chicken. | the application of crust freezing (cf) applied as a stand-alone treatment or in combination with ultraviolet (uv) light for reducing the level of artificially inoculated campylobacter jejuni on raw chicken was investigated. cf air temperatures of -5, -15 and -27 °c (±3 °c) with freezing times of 70, 15 and 6 min, respectively, were used. the level of c. jejuni on chicken was also examined following subsequent refrigerated (0-4 °c) storage at 3 and 7 days. all cf treatments resulted in significan ... | 2012 | 22850386 |
| purification and characterization of a new bacteriocin active against campylobacter produced by lactobacillus salivarius smxd51. | strain smxd51, isolated from chicken ceca and identified as lactobacillus salivarius, produced a component that inhibits the growth of gram-positive and gram-negative bacteria and especially campylobacter jejuni. the active peptide from the cell-free supernatant of lb. salivarius smxd51 was purified in three steps: (i) precipitation with 80% saturated ammonium sulfate, (ii) elution on a reversed phase spe upti-clean cartridge using different concentrations of acetonitrile, (iii) final purificati ... | 2012 | 22850384 |
| tracing campylobacter jejuni strains along the poultry meat production chain from farm to retail by pulsed-field gel electrophoresis, and the antimicrobial resistance of isolates. | in this study campylobacter jejuni isolates were recovered from birds, carcasses and carcass portions from two broiler chicken flocks and from equipment used for carcass and meat processing along the production chain from farms to retail stores. isolates were subjected to pulsed-field gel electrophoresis (pfge) using smai and kpni restriction enzymes and their antimicrobial susceptibilities were determined. c. jejuni was recovered from product and equipment used with both flocks at each point in ... | 2012 | 22850383 |
| behaviour of non-stressed and stressed listeria monocytogenes and campylobacter jejuni cells on fresh chicken burger meat packaged under modified atmosphere and inoculated with protective culture. | numerous investigations have provided evidence that chicken products are a source of listeria monocytogenes and campylobacter jejuni. different strategies applied in final products are needed to prevent consumers' contamination. in this work, the combination of modified atmosphere packaging (map) and protective culture to control the growth of freeze stressed and non-stressed l. monocytogenes and c. jejuni on fresh chicken meat burger was studied. meat burgers were inoculated with l. monocytogen ... | 2012 | 22831819 |
| reduced spread of campylobacter jejuni in broiler chickens by stimulating the bird's natural barriers. | we have tested the effect of feed structure and feeding regime to prevent the spread of the zoonotic pathogen campylobacter jejuni in broiler chicken flocks. | 2012 | 22817452 |
| distribution and genotypic characterization of campylobacter jejuni isolated from poultry in split and dalmatia county, croatia. | consumption of poultry contaminated with campylobacter jejuni has been recognized worldwide as the leading cause of campylobacteriosis. therefore, the aim of our study was to investigate the prevalence and genotype diversity of campylobacter jejuni in poultry meat intended for consumption in split and dalmatia county, which is the second biggest county in croatia. furthermore, we also wanted to discover possibly stable clones of c. jejuni appearing in different samples and periods of time, which ... | 2013 | 22805333 |
| evaluating the efficacy of an avian-specific probiotic to reduce the colonization of campylobacter jejuni in broiler chickens. | campylobacteriosis is the most frequent zoonotic disease in humans worldwide, and the contaminated poultry meat by campylobacter jejuni can be considered one of the important sources of enteric infections in humans. the use of probiotics, which can help to improve the natural defense of animals against pathogenic bacteria, is an alternative and effective approach to antibiotic administration for livestock to reduce bacterial contamination. in vitro experiments showed that enterococcus faecium, p ... | 2012 | 22802174 |
| carbohydrate binding and gene expression by in vitro and in vivo propagated campylobacter jejuni after immunomagnetic separation. | campylobacter jejuni is an important human food-borne intestinal pathogen, however relatively little is known about its mechanisms of pathogenesis or pathogen-host interactions. to monitor changes in gene expression and glycan binding of c. jejuni within a common avian host, an immunomagnetic separation technique (ims) was utilised to directly isolate infecting c. jejuni 81116 from a chicken host. an average of 10(5) cells/g was re-isolated from chicken caecal samples by ims technique. the in vi ... | 2013 | 22753110 |
| survival of campylobacter jejuni in chicken meat at frozen storage temperatures. | the aim of this study was to determine the survival of campylobacter jejuni in chicken meat samples at frozen temperatures and given length of incubation and to determine the impact of aerobic bacteria on the survival of c. jejuni. the chicken meat samples were inoculated with c. jejuni nctc 11351 suspensions and stored in bags at temperatures of -20°c and -70°c. the mean value of c. jejuni from meat samples decreased from 7.52 log10 cfu/g after 30 minutes of incubation at ambient temperature, t ... | 2012 | 22750779 |
| campylobacter jejuni is highly susceptible to killing by chicken host defense peptide cathelicidin-2 and suppresses intestinal cathelicidin-2 expression in young broilers. | little is known about the interactions of chicken host defense peptides (hdps) with campylobacter jejuni in young chicks. to examine the role of the chicken hdp, cathelicidin-2 (cath-2) in host-pathogen interactions we challenged 4-day-old ross 308 broilers with a chicken-derived c. jejuni isolate (ws356) and used the chicken pathogen salmonella enterica enteritidis phage type 4 (fgt1) as a reference. immunohistochemical staining was used to localize cath-2, c. jejuni and salmonella enteritidis. ... | 2012 | 22728124 |
| application of medium-chain fatty acids in drinking water increases campylobacter jejuni colonization threshold in broiler chicks. | campylobacteriosis is the most reported bacterial-mediated gastroenteritic disease in many developed countries. broiler chickens are a natural host for campylobacter spp., and contaminated poultry meat products are a major source for transmitting pathogenic campylobacter strains to humans. currently, no intervention measure efficiently and effectively controls this pathogen in poultry flocks. medium-chain fatty acids (caproic, caprylic, capric, and lauric acids) show a marked anti-campylobacter ... | 2012 | 22700521 |
| survival of campylobacter jejuni in chicken meat, chicken skin and chicken liver at low temperatures. | the aim of this study was to determine survival of campylobacter jejuni (c. jejuni) in chicken meat, chicken skin medallions and chicken liver contaminated with this bacterium at +4 °c and -20 °c, after 24 hours of incubation. the survival of c. jejuni at +4 °c, -20 °c in chicken meat, chicken skin medallions and chicken liver were examined after 24 hours of incubation with c. jejuni. all samples were previously tested for the presence of campylobacter species according to iso 10272-1:2006. afte ... | 2012 | 22693971 |
| anti-infective bovine colostrum oligosaccharides: campylobacter jejuni as a case study. | campylobacter jejuni is the leading cause of acute bacterial infectious diarrhea in humans. unlike in humans, c. jejuni is a commensal within the avian host. heavily colonized chickens often fail to display intestinal disease, and no cellular attachment or invasion has been demonstrated in-vivo. recently, researchers have shown that the reason for the attenuation of c. jejuni virulence may be attributed to the presence of chicken intestinal mucus and more specifically chicken mucin. since mucins ... | 2012 | 22647676 |
| a bifidobacterium-based synbiotic product to reduce the transmission of c. jejuni along the poultry food chain. | with the ban of dietary antimicrobial agents, the use of probiotics, prebiotics and synbiotics has attracted a great deal of attention in order to improve intestinal health and control food-borne pathogens, which is an important concern for the production of safe meat and meat products. recently, campylobacter jejuni has emerged as a leading bacterial cause of food-borne gastroenteritis in humans, and epidemiological evidences indicate poultry and poultry products as the main source of human inf ... | 2012 | 22608658 |
| an inactivation kinetics model for campylobacter jejuni on chicken meat under low-temperature storage. | campylobacter jejuni is susceptible to low temperatures. freezing and chilling are effective interventions for reducing the occurrence of c. jejuni on poultry meat. the survival rates of three c. jejuni strains (atcc33560, jr0706-2, and alm-80) inoculated onto chicken meat samples were measured at -20°c and 4°c, and the survival curves of these three strains were determined. the results showed that the number of surviving cells decreased by 3.16, 2.87, and 3.14 log colony-forming unit (cfu)/g, r ... | 2012 | 22568749 |
| analysis of campylobacter jejuni whole-genome dna microarrays: significance of prophage and hypervariable regions for discriminating isolates. | campylobacter is a leading cause of foodborne illness in humans, and improving our understanding of the epidemiology of this organism is essential. the objective of this study was to identify the genes that discriminate isolates of c. jejuni by analysis with whole-genome dna microarrays. statistical analyses of whole-genome data from 95 geographically diverse cattle, chicken, and human c. jejuni isolates identified 142 most significant variable genes. of this total, 125 (88%) belonged to genomic ... | 2012 | 22506962 |
| the role of wlarg, wlatb and wlatc in lipooligosaccharide synthesis by campylobacter jejuni strain 81116. | campylobacter jejuni is a major bacterial cause of gastroenteritis world-wide. c. jejuni produces a range of glycans including lipooligosaccharide (los), an important virulence factor. the genetic content of the los synthesis locus varies between c. jejuni strains and 19 classes have been described. three los synthesis genes of c. jejuni strain 81116 (nctc 11828), wlarg, wlatb and wlatc were the focus of this study. wlarg and the remaining two proteins of interest share sequence similarity to am ... | 2012 | 22445818 |
| eimeria species parasites as novel vaccine delivery vectors: anti-campylobacter jejuni protective immunity induced by eimeria tenella-delivered cjaa. | vaccination of poultry against coccidiosis caused by the eimeria species is almost entirely based upon varied formulations of live parasites. the recent development of a series of protocols that support genetic complementation by transfection in eimeria now provides an opportunity to utilise live anticoccidial vaccines to deliver additional vaccinal antigens. the capacity of eimeria tenella to express an exogenous antigen and induce an immune response during in vivo infection which is protective ... | 2012 | 22342500 |
| differentiation of the virulence potential of campylobacter jejuni strains by use of gene transcription analysis and a caco-2 assay. | campylobacter jejuni is the leading cause of bacterial diarrheal disease in humans, and contaminated poultry and poultry products are recognized as the main vehicle of infection. despite the significance of c. jejuni as a foodborne pathogen, little is known about its response to stress, and, especially, how its virulence is modulated under such conditions. the aim of this study was to assess the effect of temperature shift in a broth model system on virulence expression and cell survival of thre ... | 2012 | 22336513 |
| campylobacter, salmonella, listeria monocytogenes, verotoxigenic escherichia coli, and escherichia coli prevalence, enumeration, and subtypes on retail chicken breasts with and without skin. | this study examined the prevalence, counts, and subtypes of campylobacter, salmonella, listeria monocytogenes, verotoxigenic escherichia coli (vtec), and e. coli on raw retail chicken breast with the skin on versus the skin off. from january to december 2007, 187 raw skin-on chicken breasts and 131 skin-off chicken breasts were collected from randomly selected retail grocery stores in the region of waterloo, ontario, canada. campylobacter isolates were recovered from a higher proportion of the s ... | 2012 | 22221353 |
| high prevalence and predominance of the aph(2″)-if gene conferring aminoglycoside resistance in campylobacter. | campylobacter is a major foodborne pathogen and previous studies revealed that campylobacter isolates from food-producing animals are increasingly resistant to gentamicin in china. the molecular epidemiology and genetic mechanisms responsible for gentamicin resistance in china have not been well understood. in this study, 607 campylobacter isolates of chicken and swine origins collected in the year of 2014 were analyzed, revealing that 15.6% (25/160) of the c. jejuni isolates and 79.9% (357/447) ... | 2017 | 28264854 |
| high rates of contamination of poultry meat products with drug resistant campylobacter in metro manila, philippines. | a total of 265 chicken parts were collected from 15 wet markets and 15 supermarkets in metro manila, philippines. campylobacter spp. was isolated on modified charcoal cefoperazone deoxycholate agar plates and identified through biochemical tests and pcr amplification of genus and species specific genes. antimicrobial resistance profiles were determined following clinical and laboratory standards institute protocols. two hundred seven (78%) campylobacter spp. were isolated. c. jejuni and c. coli ... | 2016 | 27795478 |
| development of an isothermal amplification-based assay for the rapid visual detection of salmonella bacteria. | the efficient and timely detection of pathogens is a major concern worldwide. the aim of this study was to establish a rapid detection method for salmonella bacteria in food samples to facilitate timely treatment. widely used detection methods currently include culture-based methods and pcr-based methods. the former are time consuming, requiring 2 to 3 d, whereas the latter have higher accuracy but are typically complicated, requiring expertise and expensive instruments. in this study, a sensiti ... | 2017 | 28711269 |
| a rapid salmonella detection method involving thermophilic helicase-dependent amplification and a lateral flow assay. | salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. since conventional culture methods to detect salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. in this study, we developed a rapid, simple and portable salmonella detection strategy that combines thermophilic helicase-dependent amplification (thda) ... | 2017 | 28526589 |
| probiotic characteristics of lactobacillus curvatus dn317, a strain isolated from chicken ceca. | the probiotic characteristics of lactobacillus curvatus dn317, a strain isolated from chicken ceca, were evaluated. this strain was selected for study from the isolated lactobacillus strains because it has specific anti-microbial activity against campylobacter jejuni atcc 33560, camp. jejuni nctc 11168, listeria monocytogenes atcc 7644, and bacillus subtilis atcc 8633. lact. curvatus dn317 showed an auto-aggregation percentage of 72% and presented the highest co-aggregation with lact. monocytoge ... | 2017 | 28741151 |
| prevalence and risk factors for thermotolerant species of campylobacter in poultry meat at retail in europe. | the thermotolerant species campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis are the causative agents of the human illness called campylobacteriosis. this infection represents a threat for the health of consumers in europe. it is well known that poultry meat is an important food vehicle of campylobacter infection. as emerged from the reported scientific literature published between 2006 and 2016, poultry meat sold at retail level in europe represents an i ... | 2017 | 28854745 |
| isolation and identification of campylobacter spp. from poultry and poultry by-products in tunisia by conventional culture method and multiplex real-time pcr. | thermophilic campylobacter spp. are one of the primary causes of bacterial human diarrhea. the consumption of poultry meats, by-products, or both is suspected to be a major cause of human campylobacteriosis. the aims of this study were to determine the prevalence of thermophilic campylobacter spp. in fresh poultry meat and poultry by-products by conventional culture methods and to confirm campylobacter jejuni and campylobacter coli isolates by using the multiplex pcr assay. two hundred fifty fre ... | 2017 | 28853632 |
| genome reduction for niche association in campylobacter hepaticus, a cause of spotty liver disease in poultry. | the term "spotty liver disease" (sld) has been used since the late 1990s for a condition seen in the uk and australia that primarily affects free range laying hens around peak lay, causing acute mortality and a fall in egg production. a novel thermophilic sld-associated campylobacter was reported in the united kingdom (uk) in 2015. subsequently, similar isolates occurring in australia were formally described as a new species, campylobacter hepaticus. we describe the comparative genomics of 10 c. ... | 2017 | 28848714 |
| genetic diversity of thermotolerant campylobacter spp. isolates from different stages of the poultry meat supply chain in argentina. | the objective of this study was to investigate a clonal relationship among thermotolerant campylobacter spp. isolates from different stages of the poultry meat supply chain in argentina. a total of 128 thermotolerant campylobacter spp. (89 c. jejuni and 39 c. coli) isolates from six poultry meat chains were examined. these isolates were from: a) hens from breeder flocks, b) chickens on the farm (at ages 1 wk and 5 wk), c) chicken carcasses in the slaughterhouse, and d) chicken carcasses in the r ... | 2017 | 28712509 |
| a study on campylobacter jejuni and campylobacter coli through commercial broiler production chains in thailand: antimicrobial resistance, the characterization of dna gyrase subunit a mutation, and genetic diversity by flagellin a gene restriction fragment length polymorphism. | contaminated poultry meat is regarded as the main source of human campylobacteriosis. during september 2014 and february 2015, breeder flocks, hatcheries, and broiler farms from two chicken production chains were investigated chronologically. five commercial breeder flocks (breeder flocks 1-5), two hatcheries (hatcheries a and b), and five broiler flocks (broiler flocks 1-5) were sampled in this study. campylobacter colonization of both breeder and broiler flocks was determined from cloacal swab ... | 2017 | 28665716 |
| detection and prevalence of antimicrobial resistance genes in <i>campylobacter</i> spp. isolated from chickens and humans. | campylobacter spp. are common pathogenic bacteria in both veterinary and human medicine. infections caused by campylobacter spp. are usually treated using antibiotics. however, the injudicious use of antibiotics has been proven to spearhead the emergence of antibiotic resistance. the purpose of this study was to detect the prevalence of antibiotic resistance genes in campylobacter spp. isolated from chickens and human clinical cases in south africa. one hundred and sixty one isolates of campylob ... | 2017 | 28582978 |
| dissemination of erm(b) and its associated multidrug-resistance genomic islands in campylobacter from 2013 to 2015. | a total of 1372 campylobacter isolates (1107 campylobacter coli and 265 campylobacter jejuni) were obtained from 3462 samples collected from slaughterhouses and farms in three representative regions of china (shandong, guangdong, and shanghai) over three successive years (2013-2015). of these, 84 (84/1372, 6.1%) were erm(b)-positive, and all 84 positive isolates were identified as c. coli (83 chicken isolates and one swine isolate). the prevalence of erm(b) in campylobacter isolates was compared ... | 2017 | 28532801 |
| identification and differentiation of campylobacter species by high-resolution melting curve analysis. | campylobacter jejuni and campylobacter coli are the important food-born zoonotic pathogen, also are leading causes of human food borne illnesses worldwide. cadf gene is expressed in all c. jejuni and c. coli strains and mediates cell binding to the cell matrix protein, fibronectin. high-resolution melting (hrm) analysis is emerging as an efficient and robust method for discriminating dna sequence variants. the goal of this study was to apply hrm analysis to identification of c. jejuni and c. col ... | 2017 | 28478199 |
| the current state of macrolide resistance in campylobacter spp.: trends and impacts of resistance mechanisms. | campylobacter spp., especially campylobacter jejuni and c. coli, are leading bacterial foodborne pathogens worldwide. in the united states, an estimated 0.8 million cases of campylobacteriosis occur annually, mostly involving c. jejuni campylobacteriosis is generally self-limiting, but in severe cases, treatment with antibiotics may be mandated. the increasing incidence of fluoroquinolone resistance in campylobacter has rendered macrolides such as erythromycin and azithromycin the drugs of choic ... | 2017 | 28411226 |
| cleaning and disinfection programs against campylobacter jejuni for broiler chickens: productive performance, microbiological assessment and characterization. | detailed cleaning and disinfection programs aims to reduce infection pressure from microorganisms from one flock to the next. however, studies evaluating the benefits to poultry performance, the sanitary status of the facilities, and the sanitary quality of the meat are rarely found. thus, this study was designed to evaluate 2 cleaning and disinfecting programs regarding their influence on productive performance, elimination of campylobacter, and characterization of campylobacter jejuni strains ... | 2017 | 28854757 |
| the influence of the gut microbiota composition on campylobacter jejuni colonization in chickens. | campylobacter jejuni (c. jejuni)-host-interaction may be affected by the host's gut microbiota through competitive exclusion, metabolites or modification of the immune response. to understand this interaction c. jejuni colonization and local immune responses were compared in chickens with different gut microbiota composition. birds were treated with an antibiotic cocktail (at) (experiment 1 and 2) or raised under germ-free (gf) conditions (experiment 3). at 18 days post hatch (dph), they were ei ... | 2017 | 28808158 |
| animal-related factors associated with moderate-to-severe diarrhea in children younger than five years in western kenya: a matched case-control study. | diarrheal disease remains among the leading causes of global mortality in children younger than 5 years. exposure to domestic animals may be a risk factor for diarrheal disease. the objectives of this study were to identify animal-related exposures associated with cases of moderate-to-severe diarrhea (msd) in children in rural western kenya, and to identify the major zoonotic enteric pathogens present in domestic animals residing in the homesteads of case and control children. | 2017 | 28783751 |
| intrinsic and extrinsic aspects on campylobacter jejuni biofilms. | biofilm represents a way of life that allows greater survival of microorganisms in hostile habitats. campylobacter jejuni is able to form biofilms in vitro and on surfaces at several points in the poultry production chain. genetic determinants related to their formation are expressed differently between strains and external conditions are decisive in this respect. our approach combines phylogenetic analysis and the presence of seven specific genes linked to biofilm formation in association with ... | 2017 | 28769900 |
| population structure and attribution of human clinical campylobacter jejuni isolates from central europe to livestock and environmental sources. | campylobacter jejuni is among the most prevalent causes of human bacterial gastroenteritis worldwide. domesticated animals and, especially, chicken meat are considered to be the main sources of infections. however, the contribution of surface waters and wildlife in c. jejuni transmission to humans is not well understood. we have evaluated the source attribution potential of a six-gene multiplex pcr (mpcr) method coupled with structure analysis on a set of 410 c. jejuni strains isolated from envi ... | 2017 | 28755449 |
| in vitro characteristics of lactobacillus spp. strains isolated from the chicken digestive tract and their role in the inhibition of campylobacter colonization. | campylobacter jejuni/coli infections are the leading cause of bacterial diarrheal illnesses in humans. many epidemiological studies indicate that improperly prepared meat from chickens that carry a high load of campylobacter in their intestinal tracts is the key source of human infections. lab, mainly members of the lactococcus and lactobacillus genera, increasingly have been tested as vehicles for the delivery of heterologous bacterial or viral antigens to animal mucosal immune systems. thus, t ... | 2017 | 28736979 |
| β-resorcylic acid, a phytophenolic compound, reduces campylobacter jejuni in postharvest poultry. | human campylobacter infections, a leading foodborne illness globally, has been linked with the high prevalence of this bacterium on raw retail chicken products. reduction of campylobacter counts on poultry products would greatly reduce the risk of subsequent infections in humans. to this end, this study investigated the potential of the phytophenolic compound β-resorcylic acid (br) to reduce campylobacter counts on postharvest poultry (chicken skin or meat). four trials in total, two each on thi ... | 2017 | 28686495 |
| effects of dry whey powder and calcium butyrate supplementation of corn/soybean-based diets on productive performance, duodenal histological integrity, and campylobacter colonization in broilers. | campylobacter is the main cause of gastroenteritis in humans in industrialized countries, and poultry is its principal reservoir and source of human infections. dietary supplementation of broiler feed with additives could improve productive performance and elicit health benefits that might reduce campylobacter contamination during primary production. the aim of this study was to assess the effect of dietary supplementation with whey (a prebiotic) and calcium butyrate (a salt of a short-chain fat ... | 2017 | 28651615 |
| draft genome sequence of campylobacter jejuni cam970 and c. coli cam962, associated with a large outbreak of foodborne illness in fukuoka, japan, in 2016. | here, we report the draft genome sequences of campylobacter jejuni cam970 and c. coli cam962, which were associated with a large outbreak of foodborne illness originating from undercooked chicken sushi in fukuoka, japan, in may 2016. their genome sizes were 1,690,901 and 1,704,736 bp, with 22 and 23 rrnas, 9 and 9 trnas, and 411× and 419× coverage for c. jejuni cam970 and c. coli cam962, respectively. | 2017 | 28619796 |
| towards diagnostic metagenomics of campylobacter in fecal samples. | the development of diagnostic metagenomics is driven by the need for universal, culture-independent methods for detection and characterization of pathogens to substitute the time-consuming, organism-specific, and often culture-based laboratory procedures for epidemiological source-tracing. some of the challenges in diagnostic metagenomics are, that it requires a great next-generation sequencing depth and unautomated data analysis. | 2017 | 28595575 |
| the effect of microbial challenge on the intestinal proteome of broiler chickens. | in poultry production intestinal health and function is paramount to achieving efficient feed utilisation and growth. uncovering the localised molecular mechanisms that occur during the early and important periods of growth that allow birds to grow optimally is important for this species. the exposure of young chicks to used litter from older flocks, containing mixed microbial populations, is a widely utilised model in poultry research. it rarely causes mortality but effects an immunogenic stimu ... | 2016 | 28572745 |
| sourcer: classification and source attribution of infectious agents among heterogeneous populations. | zoonotic diseases are a major cause of morbidity, and productivity losses in both human and animal populations. identifying the source of food-borne zoonoses (e.g. an animal reservoir or food product) is crucial for the identification and prioritisation of food safety interventions. for many zoonotic diseases it is difficult to attribute human cases to sources of infection because there is little epidemiological information on the cases. however, microbial strain typing allows zoonotic pathogens ... | 2017 | 28558033 |
| comparing the genetic diversity and antimicrobial resistance profiles of campylobacter jejuni recovered from cattle and humans. | campylobacter jejuni, a leading cause of gastroenteritis in humans, is a foodborne pathogen that can reside in chickens, pigs, and cattle. because resistance to fluoroquinolones and macrolides, which are commonly used to treat human infections, has emerged in c. jejuni, it is imperative to continously monitor resistance patterns and examine the genetic variation in strains from human infections and animal reservoirs. our previous study of c. jejuni from human campylobacteriosis cases showed a si ... | 2017 | 28536568 |
| prevalence and characterization of campylobacter jejuni isolated from retail chicken in tianjin, china. | campylobacter jejuni is an important foodborne pathogen worldwide; however, there is a lack of information on the prevalence and antibiotic-resistant profile of c. jejuni in the people's republic of china. we determined the prevalence and characteristics of c. jejuni on the retail level in tianjin, one of the five national central cities in china. a total of 227 samples of chicken wings, legs, and breasts were collected from supermarkets and wet markets; 42 of these samples were confirmed to be ... | 2017 | 28504616 |
| cj0440c affects flagella formation and in vivo colonization of erythromycin-susceptible and -resistant campylobacter jejuni. | campylobacter jejuni is one of the most common foodborne pathogen worldwide. a putative transcriptional regulator, cj0440c, was up-regulated in the erythromycin-resistant c. jejuni, however, the precise role of cj0440c is yet to be determined. the aim of this study was to determine the biological functions of cj0440c. the cj0440c isogenic mutants were constructed from erythromycin-susceptible c. jejuni nctc 11168 (s) and -resistant c. jejuni 68-er (r), designating as sm and rm, respectively. the ... | 2017 | 28487689 |
| epidemiology of antimicrobial resistant campylobacter spp. isolated from retail meats in canada. | campylobacter is an important zoonotic pathogen found in livestock and can cause illness in humans following consumption of raw and undercooked meat products. the objectives of this study were to determine the prevalence of campylobacter spp. in retail meat (poultry, turkey, pork and beef) purchased in alberta, canada and to assess antimicrobial resistance and genetic relatedness of recovered campylobacter strains with previously isolated strains from clinical and environmental sources. a compar ... | 2017 | 28477522 |
| genome-wide fitness analyses of the foodborne pathogen campylobacter jejuni in in vitro and in vivo models. | campylobacter is the most common cause of foodborne bacterial illness worldwide. faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. there is a lack of insight into the mechanisms driving c. jejuni growth and survival within hosts and the environment. here, we report a detailed analysis of c. jejuni fitness across models reflecting stages in its life cycle. transposon (tn) gene-inactivation libraries were generated in three c. jej ... | 2017 | 28455506 |
| application of β-resorcylic acid as potential antimicrobial feed additive to reduce campylobacter colonization in broiler chickens. | campylobacter is one of the major foodborne pathogens that result in severe gastroenteritis in humans, primarily through consumption of contaminated poultry products. chickens are the reservoir host of campylobacter, where the pathogen colonizes the ceca, thereby leading to contamination of carcass during slaughter. a reduction in cecal colonization by campylobacter would directly translate into reduced product contamination and risk of human infections. with increasing consumer demand for antib ... | 2017 | 28428779 |