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molecular comparison of the dense granule proteins gra6 and gra7 of neospora hughesi and neospora caninum.neospora hughesi is a recently described apicomplexan parasite that has been associated with several cases of equine protozoal myeloencephalitis. the biology of this new parasite is just beginning to be defined. towards this understanding, we report important differences between the nucleotide and deduced amino acid sequences of the dense granule proteins gra6 and gra7 of n. hughesi and neospora caninum. this information can be used to differentiate the two species and contribute to further unde ...200111226451
seroprevalence of neospora, toxoplasma gondii and sarcocystis neurona antibodies in horses from jeju island, south korea.parasite-specific antibody responses to neospora spp. and toxoplasma gondii, antigens were detected using the indirect fluorescent antibody test (ifat) and immunoblot analysis in a korean equine population located on jeju island, south korea (126 degrees 12' e and 33 degrees 34' n). for comparison, a naturally infected neospora hughesi horse and an experimentally inoculated t. gondii equid (pony) were used. in addition, all samples were tested for antibodies to sarcocystis neurona by immunoblot ...200212062508
risk of transplacental transmission of sarcocystis neurona and neospora hughesi in california horses.the study objective was to assess the risk of transplacental transmission of sarcocystis neurona and neospora hughesi in foals from 4 california farms during 3 foaling seasons. serum of presuckle foals and serum and colostrum of periparturient mares were tested using indirect fluorescent antibody tests for s. neurona and n. hughesi. serum antibody titers were < or =10 in 366 presuckle foals tested. there was no serologic or histologic evidence of either parasite in aborted fetuses or placentas e ...200415715226
risk of postnatal exposure to sarcocystis neurona and neospora hughesi in horses.to estimate risk of exposure and age at first exposure to sarcocystis neurona and neospora hughesi and time to maternal antibody decay in foals.200415334837
description of a new neospora species (protozoa: apicomplexa: sarcocystidae).neospora hughesi n. sp. was isolated from the central nervous system tissue of an adult equine (equus caballus) from california. the tachyzoites are crescent-shaped, approximately 2 x 5 microm (1.8-3.0 x 4.0-7.0 microm), with characteristic apical complex structures consisting of an anterior polar ring, conoid, numerous rhoptries filled with a uniform electron-dense material, and 22 microtubules extending posteriorly from the polar ring. comparison of n. hughesi to canine and bovine neospora can ...19989794642
enzyme-linked immunosorbent assays for detection of equine antibodies specific to sarcocystis neurona surface antigens.sarcocystis neurona is the primary causative agent of equine protozoal myeloencephalitis (epm), a common neurologic disease of horses in the americas. we have developed a set of enzyme-linked immunosorbent assays (elisas) based on the four major surface antigens of s. neurona (snsags) to analyze the equine antibody response to s. neurona. the snsag elisas were optimized and standardized with a sample set of 36 equine sera that had been characterized by western blotting against total s. neurona p ...200516148170
a random amplified polymorphic dna polymerase chain reaction technique that differentiates between neospora species.neospora caninum is a recently described coccidial parasite that was first isolated from a dog in 1988 and has subsequently been shown to infect a wide range of mammals. neospora hughesi, a new species of this genus, has recently been isolated from the spinal cord of horses showing clinical signs of equine protozoal myeloencephalitis. the random amplified polymorphic dna polymerase chain reaction technique is capable of differentiating between n. caninum and n. hughesi.200011191920
prevalence of sarcocystis neurona and neospora spp. infection in horses from brazil based on presence of serum antibodies to parasite surface antigen.sera from 961 horses from brazil were tested for antibodies against the major surface antigens snsag4 and nhsag1 to determine the seroprevalence of sarcocystis neurona and neospora hughesi, respectively. antibodies against snsag4 were detected in 669 (69.6%) of the horses, while antibodies against nhsag1 were detected in only 24 (2.5%) of the horses. these serologic results suggest that there is a high concentration of s. neurona in the environment of brazil, which results in marked exposure of ...200616310955
endogenous transplacental transmission of neospora hughesi in naturally infected horses.abstract over a 2-yr study period, we investigated possible endogenous transplacental transmission of neospora hughesi in 74 mare and foal pairs following the diagnosis of neuronal neosporosis in a weanling foal. presuckle and postsuckle serum of each foal, serum and colostrum of each periparturient mare, and serum of each mare and foal pair, collected at 3-mo intervals thereafter, were tested for n. hughesi using an indirect fluorescent antibody test (ifat). furthermore, whole blood and colostr ...201121506870
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