Publications

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intrauterine and intrafetal inoculations with equine abortion virus in pregnant mares. 195313010086
cultivation of equine abortion virus in fetal horse tissue in vitro. 195313016787
cultivation of the equine influenza virus in suckling syrian hamsters. its similarity to the equine abortion virus. 195413127395
susceptibility in vitro of adult horse tissue to equine abortion virus; previously undescribed lesions. 195513268617
propagation of equine abortion virus in the embryonated chicken egg. 195613277328
changes in protein and nucleic acid content on hela cells infected with equine abortion virus. 195813614503
isolation of equine abortion virus from natural cases of equine abortion in horse kidney cell culture. 195914446011
quantitation and morphology of equine abortion virus in hamsters. 196014445594
the nucleic acid content of equine abortion virus. 196314025123
some physicochemical characteristics of equine abortion virus nucleic acid. 196514319712
[research into the inactivation effect of surface-active substances on the rhinopneumonitis virus in horses (equine abortion virus)]. 19685683868
on immunity to disease caused by equine herpesvirus 1. 19694307796
comparison of four horse herpesviruses.four equine herpesviruses (equine abortion virus, equine herpesvirus types 2 and 3, and equine cytomegalovirus) were compared. the equine abortion virus did not cross-neutralize with any of the other viruses, but the other three did show varying degrees of cross-neutralization among themselves. equine abortion virus grew more quickly in tissue cultures than did the others, and attained higher titers of infectivity in the culture fluid; it also formed plaques in a wider range of tissue culture sp ...19694982831
mycoplasmal deoxyribonuclease activity in virus-infected l-cell cultures.cell-free extracts of mycoplasma hominis and medium from 72-hr broth cultures had deoxyribonuclease activity like that of deoxyribonuclease i. mg(++) stimulated activity, and the ph optimum was between 8.0 and 9.0. double-stranded or heatdenatured deoxyribonucleic acid (dna) served as a substrate, and oligonucleotides were produced. cell-free extracts of l cells infected with m. hominis or m. hominis plus equine abortion virus (equine herpes virus, eav) had greatly increased activity over that o ...19695782042
evidence for a relationship between equine abortion (herpes) virus deoxyribonucleic acid synthesis and the s phase of the kb cell mitotic cycle.autoradiographic analyses of deoxyribonucleic acid (dna) synthesis in randomly growing kb cell cultures infected with equine abortion virus (eav) suggested that viral dna synthesis was initiated only at times that coincided with the entry of noninfected control cells into the s phase of the cell cycle. synchronized cultures of kb cells were infected at different stages of the cell cycle, and rates of synthesis of cellular and viral dna were measured. when cells were infected at different times w ...19714254680
effect of antiviral agents in equine abortion virus-infected hamsters.equine abortion virus, a member of the herpesvirus group, produces a lethal infection in hamsters. with this system, the protective effect of certain inhibitors of deoxyribonucleic acid viruses, inducers of interferon and exogenous interferon, was evaluated. of the various agents studied, 9-beta-d-arabinofuranosyladenine markedly suppressed mortality, and 5-iodo-2'-deoxyuridine, distamycin a, and n-ethylisatin beta-thiosemicarbazone were inactive. of the inducers tested, statolon, ultraviolet-ir ...19724376907
iododeoxyuridine and herpesviral encephalitis: lack of inhibitory action against low-grade viral replication.equine herpesvirus 1 replicated in the brains of 2-week-old mice but did not produce fatal encephalitis; it thus simulated the majority of cases of herpes simplex encephalitis in man. this replication was not inhibited by iododeoxyuridine, although in tissue cultures the equine and human viruses were equally susceptible. the continued use of iododeoxyuridine for human encephalitis should be seriously questioned.197415825425
virus-induced abortion. studies of equine herpesvirus 1 (abortion virus) in hamsters.a hamster-adapted strain of equine herpesvirus-1 (equine abortion virus) caused severe hepatic degeneration in both pregnant and nonpregnant hamsters and, in addition, regularly induced abortion in pregnant hamsters inoculated at midgestation. in nonpregnant hamsters, the only consistently affected organ was the liver despite a prolonged viremia. newborn animals usually died 1 to 2 days after inoculation; adults died 5 to 9 days after inoculation. in pregnant hamsters, the virus had a tropism fo ...1975171478
experimental studies on equine herpesvirus type 1 infections.the ehv-1 viruses of fetal origin grew better and had a wider tissue culture host range than those isolated from horses with respiratory diseases. comparisons of a fetal isolate (f/304) and a respiratory disease isolate (r/nm-3) in partly immune horses showed that the f/304 virus infected horses more readily, grew better in the nasopharynx, was more likely to cause abortion, and was excreted to a greater extent into the environment.1975173849
mitochondrial and herpesvirus-specific deoxypyrimidine kinases.to characterize and compare the thymidine (tdr) and deoxycytidine (cdr) kinase isozymes of uninfected and herpesvirus-infected cells: (i) the subcellular distribution of the isozymes has been studied; (ii) a specific assay for cdr kinase has been devised; (iii) the tdr kinase isozymes have been partially purified; and (iv) the purified enzymes have been analyzed by disc polyacrylamide gel electrophoresis, isoelectric focusing, and glycerol gradient centrifugation and by substrate competition and ...1975169387
characterization of three species of nucleocapsids of equine herpesvirus type-1 (ehv-1). 19751167720
failure to demonstrate equine rhinopneumonitis virus as a cause of abortion in mares in new zealand. 1976190568
[combined vaccination plan against influenza, equine rhinopneumonitis and tetanus for thoroughbred horses]. 1976183944
disseminated necrotizing myeloencephalitis: a herpes-associated neurological disease of horses.equine viral rhinopneumonitis type i virus was isolated from spinal cord and brain of a paraparetic horse with disseminated necrotizing myeloencephalitis. necrotic arteriolitis,nonsuppurative necrotizing myeloencephalitis and gasserian ganglioneuritis were present. on record were 12 more cases of horses with similar lesions. the horses had been ataxic or paretic for up to several weeks. a field survey indicated that 14 of 24 horses with acute myelitic signs developed them after recent exposure t ...1976180652
immunity to equine herpesvirus type 1 (rhinopneumonitis): in vitro lymphocyte response.twenty-two ponies were examined for serum-neutralizing (sn) antibody to equine herpesvirus type 1 and for in vitro lymphocyte transformation in the presence of viral antigen. six ponies had undetectable levels of neutralizing antibody (titer less than 1:2) and had lymphocytes which did not respond in culture with viral antigen (stimulation index less than 2.0). four ponies which had sn antibody to equine herpesvirus type 1 did not manifest lymphocyte transformation in vitro. the 12 remaining ser ...1976179451
equine herpesvirus in vivo: cyclic production of a dna density variant with repetitive sequences. 1976176793
abortion associated with mixed leptospira/equid herpesvirus 1 infection. 1976178087
effects of crude extracts of various plants on infectious bovine rhinotracheitis virus-plaque production.extracts of 28 plants were tested without demonstable antiviral activity in an agar-overlay plaque-reduction antiviral assay system, using infectious bovine rhinotracheitis virus and bovine endocardial cell cultures. ethanolic extract of narcissus tazetta l bulb elicited antiviral activity by inhibition of viral plaque formation. antiviral activity was demonstrated against infectious bovine rhinotracheitis and equine rhinopneumonitis viruses. narcissus tazetta l bulb did not directly inactivate ...1976176907
effect of age and pregnancy on the antibody and cell-mediated immune responses of horses to equine herpesvirus 1.the cell-mediated immune response and antibody response of horses of varying ages and of pregnant horses to equine herpesvirus 1 antigen were examined. six to eight month old horses showed either no increase or slight increases in anti-equine herpesvirus 1 serum neutralizing antibody following vaccination and revaccination with a modified live equine herpesvirus 1 vaccine. however, these same horses showed a marked increase in the cell-mediated immune response to equine herpesvirus 1 as measured ...1977200320
[several years of diagnostic studies on the ehv 1 abortion in thoroughbred studs following the introduction of vaccination]. 1977195571
respiratory disease in horses. 1977196170
equine herpesvirus 1 infection of horses: studies on the experimentally induced neurologic disease.experimental infection with equine herpesvirus 1 (rhinopneumonitis) resulted in neurologic disease in 8 of 15 inoculated horses. nonpregnant animals did not develop clinical disease, and microscopic examination of tissues revealed no changes. in all mares between 3 and 9 months of gestation, a neurologic syndrome appeared 6 to 8 days after inoculation. mares inoculated when 10 months pregnant did not develop neurologic disorders, but several aborted. the histopathologic change common to both seq ...1977195494
replication of equine herpesvirus type 1 and type 3: resistance to hydroxyurea and thymidine.the replication of equine herpesvirus type 1 (ehv-1) and type 3 (ehv-3) was unimpeded in three different cell types-equine epithelial cells, equine fibroblasts, and mouse fibroblasts-which had been blocked in their capacity to synthesize host dna by 2.5 mm hydroxyurea (hu) or 2 mm thymidine (tdr). the rate of dna synthesis in uninfected or equine herpesvirus-infected cells in the presence of hu or tdr was measured by pulse-labeling cell samples with a labeled dna precursor at different times aft ...1978563855
the protective antigens of equine herpesvirus type 1.equine herpesvirus type 1 was cultivated in swine testis cell cultures and partially purified by differential centrifugation and centrifugation in a linear sucrose density gradient. the viral envelope was separated from the nucleocapsid by treatment with rexol 25j followed by sucrose gradient centrifugation. the envelope and nucleocapsid preparations were then electrophoresed in polyacrylamide gel after solubilization with sodium dodecyl sulphate. hamsters were immunized with various preparation ...1978208736
equine rhinopneumonitis vaccine: immunogenicity and safety in foals. 1978215062
vaccination against diseases associated with herpesvirus infections in animals: a review.an account is presented of the development and use of herpesvirus vaccines in domestic animals, with particular reference to those viruses causing cytolytic rather than oncogenic infections. the chief infections covered are aujeszky's disease (ad or pseudorabies), infectious bovine rhinotracheitis (ibr) and equine rhinopneumonitis (equine abortion; ehv-1). others mentioned are feline viral rhinotracheitis and malignant catarrhal fever of cattle. both live-modified and inactivated vaccines are wi ...1978221416
oncogenic transformation of non-permissive murine cells by viable equine herpesvirus type 1 (ehv-1) and ehv-1 dna.primary cultures of balb/c mouse embryo fibroblasts infected with as much as 100 pfu per cell of ehv-1 do not exhibit cytopathology or synthesize detectable amounts of ehv-1 specific rna, dna, or infectious virus. addition of 1--2 microgram of non-fragmented ehv-1 dna as a co-precipitate with calcium phosphate to monolayers of such non-permissive mouse cells resulted in the appearance, after 4--6 weeks, of foci of piled-up, morphologically altered cells. cell lines established from such transfor ...1978221355
an outbreak of foal perinatal mortality due to equid herpesvirus type 1: pathological observations.twenty-nine cases of ehv1 infection occurred on a property, mainly in full term foals. some foals were stillborn, some were born alive but weak and soon died and others were healthy at birth, became ill and died within 3 days of birth. apart from voluminous, oedematous and atelectic lungs there were no gross lesions. microscopically the lungs showed oedema, pneumonitis and bronchiolitis with intranuclear inclusions and, in many of the foals that survived over 6 hours, there was also hyaline memb ...1979232044
differentiation of field strains and a vaccine strain of equine herpesvirus 1, using plaque characteristics. 1979223480
prevalence of antibodies to equine viruses in the netherlands.the prevalence of antibodies to various viruses was investigated in a series of serum samples collected from horses in the netherlands between 1963 and 1966 and from 1972 onwards. neutralizing antibodies to equine rhinopneumonitis virus, equine arteritis virus and to equine rhinovirus types 1 and 2 were detected in respectively 76%, 14%, 66% and 59% of the equine serum samples tested. the observed incidence of serum samples positive to equine adenovirus in the complement fixation test was 39%. p ...1979219560
equine rhinopneumonitis: more deaths--warning issued. 1979224565
oncogenic transformation by equine herpesviruses (ehv). i. properties of hamster embryo cells transformed by ultraviolet-irradiated ehv-1. 19806244694
vaccination of pregnant ponies against equine rhinopneumonitis.bovine herpesvirus 1247 (one dose) was given subcutaneously to five pregnant pony mares between 227 and 319 days of their gestations. there were no adverse clinical reactions, and the virus was not recovered from nasal swabs collected during a 2-week period after vaccination. four ponies foaled full-term, live, healthy foals. the foal of the fifth mare (no. 1) was found dead, but on the basis of the pathologic and virologic examinations, the virus was not considered to be the cause of the death. ...19806254412
herpesviral diseases affecting reproduction in the horse. 19806262988
complement requirement for virus neutralization by antibody and reduced serum complement levels associated with experimental equine herpesvirus 1 infection.pony foals, negative for detectable serum-neutralizing antibody to equine herpesvirus 1 by the standard tube-culture virus neutralization test, were experimentally infected with equine herpesvirus 1. complement-requiring (cr) and non-complement-requiring (ncr) serum-neutralizing antibodies were evaluated in preinfection and postinfection sera by means of a complement-enhanced plaque reduction assay. low levels of cr antibodies were found in the preinfection sera of only group ii ponies. upon inf ...19816260672
infection of the central nervous system of horses with equine herpesvirus serotype 1.during the last 2 years different equine herpesviruses serotype 1 strains have been isolated from cases of paretic or paralytic disease among horses in the federal republic of germany. in this paper the available information is collated and briefly reviewed. a short description of the symptoms and the possible mechanism of the pathogenesis are given.19816273556
the prospects for antiviral chemotherapy in veterinary medicine. 19816167058
antigenic and structural conservation of herpesvirus dna-binding proteins.previously, we have shown a common antigen of several herpesviruses (pseudorabies virus, equine abortion virus and bovine mammillitis virus) to be antigenically related to the major dna-binding proteins of herpes simplex virus types 1 and 2. in this study we have purified the cross-reacting polypeptide from cells infected with pseudorabies virus, equine abortion virus and bovine mammillitis virus and shown the cross-reacting protein to be a major dna-binding protein for each virus. tryptic pepti ...19816171613
[comparative studies of the efficacy of paramunity inducer pind-avi, mitogen pha-p and rhinopneumonitis virus on peripheral leukocytes in the horse]. 19816275637
serologic and molecular comparisons of several equine herpesvirus type 1 strains.the molecular and serologic relatedness of 2 recent respiratory tract isolates of equine herpesvirus type 1, designated t1 and t2, were compared with the army 183, kentucky-a hamster-adapted (kya-ha), and l-m cell-adapted (kya-lm) strains. electrophoresis in polyacrylamide gels revealed differences in virion structural proteins among 4 purified strains. seven envelope glycoproteins (molecular weight of 93,000, 65,000, 62,000, 60,000, 36,000, 20,000, and 18,000) corresponding to virion proteins 1 ...19816280520
mechanisms of infection in the respiratory tract.related to its potential vulnerability the respiratory tract has a very complex and effective defence apparatus. the interaction between these defence mechanisms and certain characteristics of aetiological agents results in a pattern in which initial infections by these agents tend to occur at specific sites in the tract. infections in which the primary portal of entry is in the upper respiratory tract include bordetella bronchiseptica and haemophilus spp in pigs; pasteurella spp in cattle, shee ...198116030806
immunogenicity of equine herpesvirus type 1 (ehv1) and equine rhinovirus type 1 (erhv1) following inactivation by betapropiolactone (bpl) and ultraviolet (uv) light.some kinetic data on the inactivation of equine herpesvirus type 1 (ehv1) and equine rhinovirus type 1 (erhv1) by betapropiolactone (bpl) and ultraviolet (uv) irradiation are reported. 0.25% bpl at 37 degrees c for 1 h reduced the titre of ehv1 by greater than 10(3 . 4) and of erhv1 by greater than 10(4 . 1) tcid50/ml. uv irradiation (334 microw/cm2) produced similar reductions in titre after 2 min. these data were used as a basis for inactivating ehv1 and erhv1 by the combined action of bpl and ...19826301141
equine influenza infections in great britain, 1979. 19826287694
variation in cellular tropism between isolates of equine herpesvirus-1 in foals.subtype-1 isolates of equine herpesvirus-1 (ehv-1) from a quadriplegic horse and from an aborted foetus were compared with each other and with a subtype-2 respiratory isolate. all 3 isolates were detected in the epithelium and macrophages of the respiratory tract. both the paresis and foetal subtype-1 isolates replicated in the epithelium of the ileum and this correlated with the recovery of virus from faeces in vivo. the paresis subtype-1 isolate also had a predelection for vascular endothelial ...19826297429
application of a chemically inactivated, adjuvanted vaccine to control abortigenic infection of mares by equine herpesvirus i.a chemically inactivated, adjuvanted vaccine prepared from a virulent strain of equine herpesvirus i (ehv-i) was used to immunize pregnant thoroughbred broodmares during a five-year field test designed to determine its safety and efficacy. each mare in the vaccinated groups received 3 intramuscular injections of vaccine beginning immediately prior to and during the last half of pregnancy. vaccine was injected at approximately 60-day intervals. the accumulated incidence of ehv-i abortions among v ...19826299858
the pathogenicity in mice of respiratory, abortion and paresis isolates of equine herpesvirus-1.eleven isolates of equine herpesvirus-1 (subtype 1) all infected the brain following intracerebral inoculation of 2 d.o. mice. most isolates were from cases of paresis, abortion or respiratory disease in the u.k., but established strains were also included. they divided into two subgroups. the 5 less pathogenic isolates were characterized by being restricted predominantly to the olfactory lobes. the 6 pathogenic isolates included the three known to cause equine paresis and were detected in neuro ...19836310851
a common code of practice for the control of contagious equine metritis and other equine reproductive diseases for the 1984 covering season in france, ireland and the united kingdom. 19836318420
ulcerative duodenitis in foals.seven foals aged 18 days to 3 1/2 months had either single or multiple full-circumference segments or long antimesenteric bands of necrotizing duodenitis, sharply delineated from adjacent viable duodenum. perforation of the necrotic wall had occurred in all foals, leading to acute fibrinous peritonitis. on the mucosal surface severe diffuse, acute inflammation and ulceration involved the anterior half of the duodenum. two further foals, aged 28 and 30 days, had lesions that are believed to be a ...19836649336
infectious center assay of intracellular virus and infective virus titer for equine mononuclear cells infected in vivo and in vitro with equine herpesviruses.a novel, simple method of infectious center assay was developed to detect and quantitate the intracellular existence of equine herpesvirus 1 and equine herpesvirus 2 in peripheral blood mononuclear cells infected in vivo and in vitro with the viruses by cocultivation of these cells with a permissive equine cell culture. the infectious center titers were correlated with the infectious virus titers. in vivo equine herpesvirus 1-infected mononuclear cells obtained from ponies experimentally infecte ...19836299486
vaccines for ehv1 abortion. 19836302971
in vivo harboring of equine herpesvirus-1 in leukocyte populations and subpopulations and their quantitation from experimentally infected ponies. 19836309042
equine immunology 4: vaccines and antisera.this paper attempts to relate the practicalities of vaccine development to the ideals which should be aimed for in a new vaccine. the type of immune response induced is dependent upon the nature of the antigen in the vaccine and the site and timing of its presentation to the immune system. in this respect the influence of age, maternal immunity and antigenic competition are discussed. the possible side effects associated with vaccination are defined and vaccines which are currently available for ...19836309511
immunologic relationships between equine herpesvirus type 1 (equine abortion virus) and type 4 (equine rhinopneumonitis virus).the specificity of selected immune responses to equine herpesvirus type 1 (ehv-1) and type 4 (ehv-4) was examined in 3 colostrum-deprived specific-pathogen-free foals. single foals were vaccinated with inactivated ehv-1, inactivated ehv-4, or control cell lysate plus adjuvant followed by successive intranasal challenge exposures with ehv-1 and ehv-4 or with ehv-4 and ehv-1. vaccination with inactivated virus preparations elicited cellular immune responses and antibody which were augmented by sub ...19846208822
trials of an inactivated equid herpesvirus 1 vaccine: challenge with a subtype 2 virus.serological responses following two and three doses of an inactivated equid herpesvirus 1 ( ehv -1) vaccine containing a subtype 1 strain were examined in yearling ponies. complement fixing antibody responses were significantly higher against the subtype 1 vaccine strain than against a subtype 2 virus. complement fixing antibody responses declined rapidly after the second dose of vaccine and had returned to almost pre-vaccination levels eight weeks after the second dose of vaccine. complement fi ...19846328729
growth kinetics of equine respiratory tract viruses in cell and organ cultures.growth kinetics of equine influenza virus-a1, equine herpesvirus-1, and equine rhinovirus-1 were determined in susceptible cell monolayers and in organ cultures of equine fetal tracheal and nasal turbinate epithelium. equine influenza virus-a1 was replicated in cell and organ cultures and was released more readily and for longer periods from nasal turbinate epithelium than from tracheal epithelium. equine herpesvirus-1 was also replicated in cell and organ cultures. during the first 24 hours aft ...19846093640
equid herpesvirus 1 (ehv 1) latency: more questions than answers. 19852996877
equine herpesvirus type 1 abortion in an onager and suspected herpesvirus myelitis in a zebra. 19853001004
isolation of equine herpesvirus 1 from the brain of a horse affected with paresis. 19853002312
antigenic and biochemical analysis of gb of herpes simplex virus type 1 and type 2 and of cross-reacting glycoproteins induced by bovine mammillitis virus and equine herpesvirus type 1.an antiserum was produced to the oligomeric form of glycoprotein b (gb) induced by herpes simplex virus type 1 (hsv-1) strain 17. this antiserum gave a single common precipitin line in agar gel immunodiffusion with hsv-1, hsv-2, bovine mammillitis virus (bmv) and equine herpesvirus type 1 (ehv-1). it also neutralized hsv-1, hsv-2 and bmv but not ehv-1. absorption of the antiserum with excess hsv-2 or bmv antigen resulted in an hsv-1-specific neutralizing antiserum. in immunoprecipitation, two pr ...19852981966
neurologic diseases.the responses of apparently healthy newborn foals to neurologic testing differ significantly from those of adult horses. these responses and the diagnostic techniques pertinent to neurologic problems are reviewed as a basis for evaluation of the compromised neonatal foal. the more frequently encountered neurologic diseases are discussed in a problem-oriented format. these clinical problems include behavioral abnormalities, convulsions, changes in consciousness, blindness, ataxia without loss of ...19853000543
antibodies against equine herpesvirus 1 in the cerebrospinal fluid in the horse.neutralizing antibodies against equine herpesvirus 1 were measured in serum and cerebrospinal fluid of 16 horses and ponies from a closed herd both before and after vaccination with modified live equine herpesvirus 1. these titers were also measured in 22 neurologically normal and 15 neurologically abnormal horses at a teaching hospital. animals from the closed herd had prevaccination serum titers up to 1:8 and postvaccination serum titers up to 1:128. horses from the teaching hospital had serum ...198517422553
equine herpesvirus type 1 infection--a reply. 198616031308
abortion and perinatal foal mortality associated with equine herpesvirus type 1 in a herd of grevy's zebra. 19862851577
foetal and neonatal foal losses on equine herpesvirus type 1(ehv-1) infected farms before and after ehv-1 vaccination was introduced.on 6 farms with a history of ehv-1 abortions the overall prevalence of losses due to abortion, stillbirth and neonatal foal mortality during 5 foaling seasons preceding the introduction of vaccination against ehv-1 were compared with corresponding data for 8 subsequent seasons in which all horses were immunized with a modified live ehv-1 vaccine. although both sporadic and epizootic ehv-1 abortions occurred in immunized mares, the numbers of foetal and neonatal foal losses decreased significantl ...19862830601
innate immunity during equid herpesvirus 1 (ehv-1) infection.intrinsic phagocytosis and killing of c. albicans by equine monocytes and polymorphonuclear leucocytes (pmn) was examined during equid herpesvirus 1 (ehv-1) (subtypes 1 or 2) and adenovirus infections. monocyte function increased during ehv-1 subtype 2 and adenovirus infection. conversely, there was an impairment of monocyte ingestion during ehv-1 subtype 1 infection which was ascribed to virus replication in peripheral blood mononuclear cells. pmn phagocytosis was not decreased in any of the in ...19862431815
heat-inactivated frog virus 3 selectively inhibits equine herpesvirus type 1 translation in a temporal class-dependent manner.superinfection of equine herpesvirus type 1 (ehv-1)-infected rabbit kidney cells with heat-inactivated frog virus 3 (delta fv3) differentially blocked ehv-1 protein synthesis. the extent of inhibition varied with the specific ehv-1 message, but in general late protein synthesis was inhibited more than early and immediate early translation. since fv3 has been shown to block heterologous rna and protein synthesis, it was necessary to determine whether the observed reduction in herpesvirus protein ...19863727402
[application of the passive hemagglutination test to the study of equine rhinopneumonitis. ii. serologic study in horses]. 19863035677
genomic and antigenic comparison of an equine herpesvirus 1 (ehv 1) isolate from the 1983 lippizan abortion storm with ehv 1 reference strains.an ehv 1 isolate from the lippizan stud at piber, which caused the abortion and paresis outbreak in 1983, was investigated using 3 known subtype 1 and 2 subtype 2 strains for comparison. broad-scale restriction enzyme analysis as well as cross-neutralization with hyperimmune sera produced in rabbits were performed, and sds-page of infected cell proteins was conducted on a limited scale. the piber isolate was clearly classified as a subtype 1 strain of ehv 1, and showed closest resemblance in its ...19863012290
equine herpesvirus genomes: heterogeneity of naturally occurring type 4 isolates and of a type 1 isolate after heterologous cell passage.the restriction endonuclease dna fingerprints of 20 low passage, epidemiologically unrelated isolates of equine herpesvirus 4 (equine rhinopneumonitis virus) showed considerable heterogeneity in certain fragments, the positions of which were assigned to quite restricted positions on the 141 kilobase (kb) genome. we note that the heterogeneity observed in the restriction endonuclease dna fingerprints of ehv 1 (equine abortion virus) and of pseudorabies virus also tend to map to these same restric ...19863022687
segmental atresia of the transverse colon in a foal with concurrent equine herpes virus-1 infection.segmental atresia of the transverse colon was observed at necropsy in a neonatal foal. the dorsal and ventral components of the large colon were fused, and ended blindly. the small colon was collapsed and completely closed at its cranial end. the right and left dorsal and ventral colons were fused into one blind-ended tube. histologically, eosinophilic intranuclear inclusion bodies demonstrative of equine rhinopneumonitis were present in the thymus.19873032513
[infectious course of equine herpesvirus 1 infection in a riding stable]. 19873032556
use of lambda gt11 and monoclonal antibodies to map the genes for the six major glycoproteins of equine herpesvirus 1.to localize the genes for the major glycoproteins of equine herpesvirus 1 (ehv-1), a library of the ehv-1 genome was constructed in the lambda gt11 expression vector. recombinant bacteriophage expressing ehv-1 glycoprotein epitopes as fusion products with beta-galactosidase were detected by immunoscreening with monoclonal antibodies specific for each of six ehv-1 glycoproteins. seventy-four recombinant lambda gt11 clones reactive with ehv-1 monoclonal antibodies were detected among 4 x 10(5) pha ...19873037108
demonstration of equine herpes virus 1 (ehv-1) in histological sections and tissue cultures by the peroxidase-antiperoxidase (pap) technique. 19872830744
neuropathogenicity for suckling mice of equine herpesvirus 1 from the lipizzan outbreak 1983 and of selected other ehv 1 strains. 19872830747
[new observations in the diagnosis of ehv (equine herpes virus) abortions].over the last years the percentage of foals lost by ehv at term or close to term seems to be higher than in former years. furthermore, the pathological findings seem to shift from liver to lung. so far there has been no explanation for this phenomenon.19872831640
a suspected case of equine herpesvirus 1 encephalomyelitis. 19872833882
serological and virological investigations of an equid herpesvirus 1 (ehv-1) abortion storm on a stud farm in 1985.an extensive outbreak of ehv-1 abortions occurred on a stud farm in england in 1985. of the 67 pregnant mares present on the stud farm, 31 were challenged with ehv-1, resulting in the loss of 22 fetuses or foals. laboratory investigations revealed that the spread of the virus closely followed movement of apparently healthy mares (during the incubation period of the infection). during the outbreak mares were challenged 1-4 months before the expected foaling date. there was no relationship between ...19872824770
characterization of two equine herpesvirus (ehv) isolates associated with neurological disorders in horses. 19872829471
isolation and characterization of monoclonal antibodies against an attenuated vaccine strain of equine herpesvirus type 1 (ehv-1).the production and differentiation of monoclonal antibodies (mabs) against the rac-h strain of ehv-1 used as an attenuated live vaccine to prevent rhinopneumonitis and abortion is described. seven different antigenic sites were detected by the 15 mabs produced. ehv-1 specific mabs as well as ehv-1 and -4 common mabs could be established, allowing easy typing of ehv isolates. one mab recognized the vaccine strain only. this reaction was used to investigate a possible involvement of the vaccine st ...19882847404
agents of equine viral encephalomyelitis: correlation of serum and cerebrospinal fluid antibodies.a survey was conducted by testing 115 paired equine serum and cerebrospinal fluid samples by hemagglutination-inhibition for antibodies to powassan and snowshoe hare viruses, and by virus neutralization for antibodies to equine herpesvirus type 1. twenty-five samples were from horses with spontaneous neurological disease and the remainder from horses euthanized because of various nonneurological disorders. all sera and cerebrospinal fluids were negative for antibodies to powassan virus. fifty-on ...19882836046
equine rhinopneumonitis. 19892543118
identification of the gb homologues of equine herpesvirus types 1 and 4 as disulphide-linked heterodimers and their characterization using monoclonal antibodies.equine herpesvirus types 1 and 4 (ehv-1 and ehv-4) labelled with [14c]glucosamine were purified from infected cell culture medium and profiles of their structural proteins were obtained that enabled identification of the major glycoproteins. nine glycosylated polypeptides were identified for each virus. preparations of the purified viruses each contained a glycoprotein which was linked by disulphide bonds, as determined by diagonal gel electrophoresis under reducing/non-reducing conditions. high ...19892543773
nucleocapsid mass and capsomer protein stoichiometry in equine herpesvirus 1: scanning transmission electron microscopic study.the brookhaven scanning transmission electron microscope was employed to measure the masses of two nucleocapsid species (of light and intermediate densities) of equine herpesvirus 1. these were found to be 196.7 +/- 9.2 and 229.0 +/- 9.5 megadaltons (mda), respectively. biochemical assays showed that neither nucleocapsid contained any significant amount of dna (less than 0.2% [wt/wt]). taking into account data on protein composition, we conclude that the difference between their masses is essent ...19892760983
equine herpesvirus. 19892546317
mapping the termini and intron of the spliced immediate-early transcript of equine herpesvirus 1.equine herpesvirus 1 (ehv-1) has been shown to synthesize a 6.0-kilobase (kb) species of immediate-early (ie) mrna in productively infected cells. this ie gene region maps within the outer portion (map units 0.79 to 0.83 and 0.96 to 1.00) of the two inverted repeat segments of the short genomic region, and elucidation of its dna sequence has revealed multiple potential open reading frames (orfs), including a major orf of 4,461 nucleotides (f. j. grundy, r. p. baumann, and d. j. o'callaghan, viro ...19892555546
expression in recombinant vaccinia virus of the equine herpesvirus 1 gene encoding glycoprotein gp13 and protection of immunized animals.the equine herpesvirus 1 (ehv-1) gene encoding glycoprotein 13 (gp13) was cloned into the hemagglutinin (ha) locus of vaccinia virus (copenhagen strain). expression of the gp13 gene was driven by the early/late vaccinia virus h6 promoter. metabolically radiolabeled polypeptides of approximately 47 and 44 kilodaltons and 90 kilodaltons (glycosylated form) were precipitated with both polyclonal and gp13-specific monoclonal antibodies. presentation of gp13 on the cytoplasmic membrane of cells infec ...19892550665
towards a vaccine against equine herpesvirus 1. 19892559691
evidence for allelic exclusion in chinese hamster ovary cells.earlier results suggested that the functional hemizygosity of genes in pseudodiploid chinese hamster ovary (cho) cells is due to the silencing of one allele by dna methylation. from this one could make a strong prediction that we have now been able to confirm by genetic experiments, using thymidine kinase (tk) alleles. tk- mutants induced by ethylmethane sulphonate (ems) were all revertible to tk+ at high frequency by the demethylating agent 5-azacytidine (5-aza-cr). this revertibility was due t ...19901704254
coexpression by vaccinia virus recombinants of equine herpesvirus 1 glycoproteins gp13 and gp14 results in potentiated immunity.the equine herpesvirus 1 glycoprotein 14 (ehv-1 gp14) gene was cloned, sequenced, and expressed by vaccinia virus recombinants. recombinant virus vp613 elicited the production of ehv-1-neutralizing antibodies in guinea pigs and was effective in protecting hamsters from subsequent lethal ehv-1 challenge. coexpression of ehv-1 gp14 in vaccinia virus recombinant vp634 along with ehv-1 gp13 (p. guo, s. goebel, s. davis, m. e. perkus, b. languet, p. desmettre, g. allen, and e. paoletti, j. virol. 63: ...19902157895
genomic termini of equine herpesvirus 1.after cell infection with the equine herpesvirus 1 (ehv-1), the termini of the linear double-stranded dna genome fuse to form circular forms. to investigate the mechanisms in the generation and cleavage of such replicative-form dnas, the genomic termini, the fusion of termini from replicative-form molecules, and the junction between the short and long genome segments have been analyzed by restriction mapping, blot hybridizations, cloning, and sequencing. the data suggest that the genome ends are ...19902153249
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