Publications

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non-specific alkaline phosphomonoesterases of eight species of digenetic trematodes.alkaline phosphatases from different trematodes occupying the same habitat have identical ph otima but different levels of enzyme activities. isoparorchis hypselobagri, from the fish wallago attu, shows four to six times more enzyme activity than fasciolopsis buski, gastrodiscoides hominis and echinostoma malayanum, from the pig sus scrofa, and fasciola gigantica, gigantocotyle explanatum, cotylophoron cotylophorum and gastrothylax crumenifer, from the buffalo bubalus bubalis. at least two peaks ...19751442
quantitative studies on acetylcholinesterase in seven species of digenetic trematodes.quantitative estimation of absolute levels and in vitro release of acetylcholinesterase (ache) in seven species of digenetic trematodes: isoparorchis hypselobagri from the swim bladder of catfish, wallago attu; srivastavaia indica and gastrothylax crumenifer from the rumen, and gigantocotyle explanatum from the liver of the water buffalo, bubalus bubalis; fasciolopsis buski, echinostoma malayanum from the small intestine and gastrodiscoides hominis from the caecum of the pig, sus scrofa reveale ...1977562036
alkali denaturation of oxyhaemoglobins of some digenetic trematodes and their hosts.oxyhaemoglobins of six digenetic trematodes,--srivastavaia indica, gastrothylax crumenifer, gigantocotyle explanatum, fasciolopsis buski, gastrodiscoides hominis, isoparorchis hypselobagri, and of their 3 vertebrate hosts, bubalus bubalis, sus scrofa, wallago attu, were subjected to alkali denaturation at a ph of 12.4. all oxyhaemoglobins from trematodes and their hosts differ from each other in the rate and extent of alkali denaturation which may be explained due to variations in the amino acid ...1977599278
spectrophotometric analysis of haemoglobins of some digenetic trematodes and their hosts.the haemoglobins of six different species of trematodes: gastrothylax crumenifer, srivastavaia indica, gigantocotyle explanatum, fasciolopsis buski, gastrodiscoides hominis, isoparorchis hypselobagri and their three different hosts: bubalus bubalis, sus scrofa, and wallago attu were spectrophotometrically investigated, and were found to contain porphyrin ix as the common prosthetic group. oxyhaemoglobin, carbonmonoxy-haemoglobin and reduced haemoglobin of all 6 species of trematodes and their 3 ...19761010924
comparison of the hemoglobins of the platyhelminths gastrothylax crumenifer and paramphistomum epiclitum (trematoda: paramphistomatidae).1. gastrothylax crumenifer and paramphistomum epiclitum parasitize the water buffalo bubalus bubalis. 2. gastrothylas hemoglobin consisted of two fractions of ca 30,000 and ca 18,000 by gel filtration. sds-electrophoresis showed both to be single, ca 15,000 chains. 3. paramphistomum hemoglobin was ca 16,000 by both gel filtration and sds-electrophoresis. 4. reversed-phase chromatography of carboxymethylated trematode and buffalo globins gave single peaks and two peaks, respectively. although par ...19921611885
lactate dehydrogenase in two digenetic trematodes and their host.polyacrylamide gel electrophoresis of the two digenetic trematodes, gigantocotyle explanatum from the liver and gastrothylax crumenifer from the rumen of the water buffalo, bubalus bubalis revealed the presence of at least six and seven isoenzymes of lactate dehydrogenase (ldh), respectively in a partially purified enzyme preparation. the respective host tissues showed five isoenzymes of ldh, which are characteristic to the vertebrates. both parachloromercuribenzoate and iodoacetate affected the ...19902074142
studies on the ultrastructure and histochemistry of the lymph system in three species of amphistome (trematoda: digenea) gigantocotyle explanatum, gastrothylax crumenifer and srivastavaia indica from the indian water buffalo bubalus bubalis.the lymph system of three amphistome parasites from buffaloes, gigantocotyle explanatum, gastrothylax crumenifer and srivastavaia indica was studied using light microscope histochemistry and electron microscopy. in each case the system comprised a single pair of main longitudinal vessels which gave rise to numerous sub-dividing lateral branches. although the finer lymph channels associated with most internal systems, they did not penetrate the basement membrane of any organ. the lymph vessels we ...19853989258
histochemical and electrophoretic studies on phosphatases of some indian trematodes.the isoenzymes of acid and alkaline phosphatases and their histochemical localization were studied by polyacrylamide disc gel electrophoresis in four species of trematodes: gigantocotyle explanatum from the liver and gastrothylax crumenifer from the rumen of water buffalo (bubalus bubalis) and echinostoma malayanum and fasciolopsis buski from the small intestine of the pig (sus scrofa). both acid and alkaline phosphatases were present in the tegument, gastrodermis, suckers, testes, ovary, eggs, ...19827096966
effects of some sugars, amino acids, glycerol and alpha-glycerophosphate on oxygen uptake of some digenetic trematodes.effect of various substrates on the oxygen uptake of some digenetic trematodes -- srivastavaia indica, gastrothylax crumenifer, gigantocotyle explanatum from the buffalo, bubalus bubalis and isoparorchis hypselobagri from the fish wallago attu was studied. in all the four species, the effect of various substrates except maltose was found to be stimulatory. the inhibitory or stimulatory effect of these substrates on oxygen uptake reflects the importance of these compounds in the energy metabolism ...19807450612
trematode hemoglobins show exceptionally high oxygen affinity.ligand binding studies were made with hemoglobin (hb) isolated from trematode species gastrothylax crumenifer (gc), paramphistomum epiclitum (pe), explanatum explanatum (ee), parasitic worms of water buffalo bubalus bubalis, and isoparorchis hypselobagri (ih) parasitic in the catfish wallago attu. the kinetics of oxygen and carbon monoxide binding show very fast association rates. whereas oxygen can be displaced on a millisecond time scale from human hb at 25 degrees c, the dissociation of oxyge ...19989675199
functional differentiation in trematode hemoglobin isoforms.the hbs and the major electrophoretic hb components (isohbs) were isolated from three species of the trematodes, explanatum explanatum (ee), gastrothylax crumenifer (gc) and paramphistomum epiclitum (pe), that parasitise the common indian water buffalo bubalus bubalis. the hbs are monomeric and resemble the so-called nonfunctional mutant hemoglobins that have tyr at b10 or e7 positions (replacing leu and the his residues, respectively). however, they are capable of binding with o2 and co. o2 equ ...199910103000
partial purification and characterization of gastrothylax crumenifer somatic antigens.the soluble extracts of gastrothylax crumenifer isolated from the rumen of buffalo (bubalus bubalis) were fractionated on a sephadex g-200 column. a total of eight major fractions (f1, f2, f3, f4, f5, f6, f7, and f8) were separated from the whole homogenate of g. crumenifer, and each of these fractions was tested for their antigenicity by elisa against rabbit hyperimmune sera. it was observed that f1, f2, f3 and f4 were highly antigenic, f6 and f7 were moderately antigenic and f5 and f8 were poo ...200010729642
immunodetection of coproantigens for the diagnosis of amphistomosis in naturally infected indian water buffalo, bubalus bubalis.the infection of gastrointestinal helminths in livestock is routinely diagnosed by microscopical examination of faecal samples for the presence of ova/eggs but this approach becomes ineffective for the seasonally egg producing trematodes. therefore, an alternative approach to detect the coproantigens of liver and rumen amphistomes, gigantocotyle explanatum and gastrothylax crumenifer respectively, infecting indian water buffalo bubalus bubalis, was undertaken using elisa, immunodot and countercu ...201323036808
characterization of the glutamate dehydrogenase activity of gigantocotyle explanatum and gastrothylax crumenifer (trematoda: digenea).glutamate dehydrogenase (gldh) (ec 1.4.1.3) is a ubiquitous enzyme, which is present at the protein and carbohydrate metabolism crossroads. the enzyme activity was investigated in biliary and rumen amphistomes, gigantocotyle explanatum and gastrothylax crumenifer, respectively, infecting the indian water buffalo bubalus bubalis. the enzyme activity was consistently higher in g. explanatum as compared to g. crumenifer, where nad(h) was utilized as coenzyme and the ph optima was recorded at 8. the ...200923129890
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