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carrier rate of pasteurella multocida in a cattle herd associated with an outbreak of haemorrhagic septicaemia in the sudan. 197898205
biochemical and serological studies on pasteurella multocida isolated from cattle in the sudan.of 42 strains of pasteurella meltocida isolated from different outbreaks of hemorrhagic septicaemia and from healthy cattle in various parts of the suden, 38 belonged to carters' type b and four to type e. the strain used for vaccine production was type e. with respect to somatic antigen determination, there was some correlation between the results of the gel diffusion and the agglutination tests as most strains reacted with 0 groups 6 antisera in both tests. however, some antigens cross-reacted ...1979110516
[effect of aspirin and indomethacin on changes caused by pateurella multocida toxins in calves].acetylsalicyclic acid acid (50 mg/kg die) and indomethacin (1.5 mg/kg die) were administered to calves three days prior to intravenous application of endotoxin. such treatment resulted in delayed occurence of clinical symptoms and higher survival rates, as compared to untreated controls. parameters relating to coagulation physiology, changed by endotoxin, were not controllable with statistical security by administration of acetylsalicyclic acid indomethacin.1978666518
[use of attenuated mutants of pasteurella multocida strain, pathogenic to calves, in experimental mouse model studies. 4. immunogenicity of selected revertants of temperature-sensitive, streptomycin-dependent mutants as potential vaccine strains].temperature-resistant or streptomycin-independent revertants were isolated from temperature-sensitive or streptomycin-dependent mutants of a pasteurella multocida strain with pathogenicity to calf. the revertants were tested for virulence in mice and for their immunogenicity against wild-strain infection, using 100 times and more of the lethal dose. the following conclusions may be derived from the results thus obtained: 1. most of the fully attenuated streptomycin-independent revertants failed ...1978749742
[influence of pasteurella multocida and pasteurella-multocida endotoxin on the blood-coagulation-analytic parameter after experimental administration in calves].experimental administration to calves of pasteurella multocida or the application of an endotoxin preparation of the strain produced continuous decline of thrombocytes, disorders in thrombocytic functions, and the development of hypofibrinogenaemia. the changes recorded as well as the clinical symptoms and pathologico-anatomic findings are interpreted as endotoxin shock.1977901129
phycomycosis associated with encephalitis caused by haemophilus somnus in a heifer.numerous fungal hyphae resembling those of a phycomycete were found in thrombi, vessel walls, and areas of inflammatory cell infiltration within a large focus of necrosis in the brainstem of a 2-year-old heifer. haemophilus somnus and pasteurella multocida were isolated from the same lesion. antemortem hyperglycemia was also demonstrated.1977903285
physiologic characteristics of 1,268 cultures of pasteurella multocida.results of 29 physiologic tests are reported for 1,268 cultures of pasteurella multocida from various hosts over a 10-year period. of the cultures, 97 to 100% fermented galactose, glucose, mannitol, mannose, fructose, and sucrose, produced hydrogen sulfide and indole, and reduced nitrate; 6 to 91% fermented arabinose, glycerol, sorbitol, trehalose and xylose. fermentation of dextrin, dulcitol, inositol, inulin, lactose, maltose, raffinose, rhamnose, and salicin, growth on macconkey agar, change ...1976937797
bronchiectasis in yearling feedlot cattle.thoughout all of 1974, we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle. from the 3,943 dead cattle, 1,988 necropsies were made; of the cattle necropsied, 32 (1.6%) had bronchiectasis. in this disease, the permanently dilated small bronchi and bronchioles, located in ventral parts of the lungs, were filled with accumulations of exudate and microorganisms, including pasteurella hemolytica, pasteurella multocida, corynebacterium pyogenes, escherichia coli, salmonella an ...1976956033
[murine model for experimental studies on attenuated mutants of a pasteurella multocida strain with pathogenicity for the calf. 1. isolation and biological characterization of streptomycin-dependent mutants (sm-d) and their examination for immunogenicity in comparison with thermally inactivated pathogens].an experimental study was conducted into the immunisation of mice to a strain of pasteurella multocida with pathogenicity for calf. here are the results: (a) stable sm-d mutants were isolated. (b) the genetic stability of this mutant type was established by unsubstantiable reversion up to the magnitude of 10(-8) or - in cases in which 20 sm-id revertants maximum occurred in 10(-8) sm-d germs--by prolongation of the generation period of these suppressor mutants which can be defined also as minus ...19761035491
properties of free endotoxin from pasteurella multocida.free endotoxin (fet) from virulent encapsulated pasteurella multocida or from an avirulent nonencapsulated mutant is capable of inducing active immunity, but the lipopolysaccharide (lps) moiety of the endotoxin is not. these results suggest that a protein of p multocida is involved in the stimulation of active immunity. the serologic specificity of the fet is associated with the lps moiety, which is related to a heat extracted antigen that is used for serotyping p multocida. the fet is capable o ...19751092224
diverse effects of bursectomy on humoral immune responses in the chicken.chickens bursectomized hormonally or surgically at various ages in embryonic to postembryonic lives were immunized with a mixture of antigens, and the antibodies produced titrated. immune responses to salmonella pullorum (sp), brucella abortus (ba). staphylococcus aureus (staph) and ovalbumin (oa) were more markedly suppressed by the bursal deprivation than those to heterogenous erythrocytes, viruses and such bacteria as pasteurella multocida (pm) and escherichia coli b. the immune response to b ...19751187514
isolation of a protective, non-toxic capsular antigen from pasteurella multocida, types b and e.separation of the capsular antigen and endotoxin from saline extracts of pasteurella multocida type b was achieved by fractional precipitation from aqueous solution by addition of polar organic solvents. biological tests for the presence of endotoxin showed that it was absent from capsular antigen preparations so obtained. properties of the capsular antigen suggested that it was a high molecular weight acidic polysaccharide. the solvent fractionation method was found to be equally applicable to ...19761257631
ovine pulmonary surfactant induces killing of pasteurella haemolytica, escherichia coli, and klebsiella pneumoniae by normal serum.pulmonary surfactant has been shown to play an increasingly important role in bacterial clearance at the alveolar surface in the lung. this study describes a bactericidal mechanism in which ovine pulmonary surfactant induces killing of pasteurella haemolytica by normal serum. to demonstrate killing, six bacterial species were incubated first with pulmonary surfactant for 60 min at 37 degrees c and then with serum for an additional 60 min at 37 degrees c. p. haemolytica type a1 strains 82-25 and ...19921452351
pharmacokinetic evaluation of ceftiofur in serum, tissue chamber fluid and bronchial secretions from healthy beef-bred calves.ceftiofur is a new broad spectrum cephalosporin marketed for the treatment of acute bovine respiratory disease. in this investigation ceftiofur was administered by intramuscular injection, at 24 h intervals, to healthy beef-bred calves for four days at dosages of 2.2 and 4.4 mg/kg of body weight, with 4 wk intervals between dosing regimens. serum, tissue chamber fluid (tcf), and bronchial secretion (bs) concentrations of ceftiofur were measured by microbiological assay after the first and fourth ...19921477795
[the importance of dermonecrotoxin and dermonecrotoxoid of pasteurella multocida for the calf].the i. m. application of dermonecrotoxin of p. multocida var. d led to an atrophy of nasal conchae, liver-swelling and to induration of liver and spleen. the intratracheal application caused a lobular pneumonia. the vaccination with toxoid resulted in an immunity against a challenge with toxin but not with p. multocida var. d. correlations between antitoxic serum-titers and immunity have to be investigated in future.19921497586
chronic frontal sinusitis in dairy cattle: 12 cases (1978-1989).chronic frontal sinusitis in 12 dairy cattle most often was associated with a history of dehorning, in which the sinus was entered (67%), or with respiratory tract disease (25%). the most common organisms isolated were actinomyces pyogenes and pasteurella multocida. signs of infection did not develop for months in some cattle and were often intermittent. the most common clinical signs included anorexia, lethargy, fever, frontal bone distortion, exophthalmos, abnormal posture, nasal discharge, an ...19921500335
characterization and comparison of antimicrobial susceptibilities and outer membrane protein and plasmid dna profiles of pasteurella haemolytica and certain other members of the genus pasteurella.the outer membrane protein (omp), plasmid, and antimicrobial resistance profiles of pasteurella haemolytica serotypes 1 through 12, a bovine isolate of p multocida, a chicken isolate of p multocida, and an unidentified pasteurella species of bovine origin were examined. isolates of p haemolytica serotypes belonging to the same biotype possessed similar omp profiles. biotype a isolates contained 2 prominent omp of 43 kilodaltons (kd) and 29 kd, whereas biotype-t serotypes contained 3 major omp of ...19911789515
in vitro binding of pasteurella multocida cell wall preparations to tracheal mucus of cattle and swine and to a tracheal epithel cell wall preparation of cattle.outer membrane preparations of various pasteurella isolates (pasteurella multocida and some other pasteurella species) from cattle and swine were extracted by n-lauryl-sarcosine sodium salt. capsular extracts were prepared by heat treatment. both preparations bound to epithel cell wall preparations (ecw) of trachea from cattle and to tracheal mucus of cattle and swine. binding was demonstrated by an enzyme-linked immunosorbent assay (elisa). distinct high adherence values were shown by the great ...19911796684
evidence for non-siderophore-mediated acquisition of transferrin-bound iron by pasteurella multocida.two clinical isolates of pasteurella multocida associated with bovine pneumonia were examined for iron acquisition. both isolates were capable of obtaining iron for growth from bovine but not from human, avian, equine or porcine transferrin. this correlated with specific binding of bovine transferrin by iron-limited cells or isolated membranes. no siderophore was detected in the strains by a general screening assay. in response to iron-limited conditions, a number of high molecular mass iron-reg ...19911839048
interactions of haemophilus-actinobacillus-pasteurella bacteria with phagocytic cells.the haemophilus-actinobacillus-pasteurella (hap) group of bacteria contains a number of important veterinary and human pathogens. although each species has specific characteristics and host range, most share the general property of being resistant to cellular defense mechanisms. in some cases (e.g. pasteurella multocida, pasteurella haemolytica and actinobacillus pleuropneumoniae) resistance results in part from the presence of an antiphagocytic capsule that protects the bacilli against ingestio ...19902193702
our understanding of the pasteurellaceae.with the exception of a few consistent pathogens--pasteurella multocida strains of bovine hemorrhagic septicemia and fowl cholera, actinobacillus (haemophilus) pleuropneumoniae, haemophilus aegyptius and haemophilus paragallinarum--members of the family pasteurellaceae are commensal parasites on mucous membranes of vertebrate animals. many have pathogenic potential, which becomes manifest under conditions of immunodeficiency and stress. pathogenesis (except in porcine atrophic rhinitis) depends ...19902193710
specificity of dna probes for the detection of toxigenic pasteurella multocida subsp. multocida strains.five dna probes directed against different regions of the gene that encodes the dermonecrotic toxin of pasteurella multocida subsp. multocida were examined for their ability to identify toxigenic p. multocida subsp. multocida strains. the specificities of the probes were studied with 96 strains of p. multocida subsp. multocida and 22 strains of 11 other bacterial species. results of colony hybridization assays using these probes indicated that two of the five probes have potential diagnostic val ...19902203816
bovine recombinant granulocyte-macrophage colony-stimulating factor enhancement of bovine neutrophil functions in vitro.neutrophils were purified from blood of dexamethasone-treated (0.04 mg/kg of body weight) and untreated calves. cells were untreated (controls) or cultured in media containing 5 or 10 ng of bovine recombinant granulocyte-macrophage colony-stimulating factor (rbgm-csf)/ml for 10 to 12 hours before being tested for various functions. dexamethasone treatment of calves decreased luminol-dependent chemiluminescence, decreased phagocytosis of pasteurella multocida and several staphylococcus spp by var ...19902204299
bactericidal activity in the sera of mice vaccinated with pasteurella multocida type a.the susceptibility of pasteurella multocida to killing by serum and the ability of protective vaccines to stimulate this mechanism of immunity in mice were investigated. p. multocida type of bovine origin was used to prepare a vaccine incorporating heat killed organisms and for homologous infection of mice. bactericidal capacity and elisa antibody titres were determined for individual mouse sera. protection was clearly associated with bactericidal antibodies raised by vaccination. the bactericid ...19902219665
[atrophic rhinitis in swine and other animal species].evidence that cattle, goats and rabbits may suffer from natural diseases equivalent to porcine atrophic rhinitis is presented. etiology and course of the progressive (enzootic) and non-progressive (sporadic) forms of atrophic rhinitis in pigs are discussed and compared with known data about similar diseases in other animals. it seems that atrophic rhinitis caused by toxigenic strains of pasteurella multocida may be a disease of different animal species.19902242733
outer membrane proteins of bovine strains of pasteurella multocida type a and their doubtful role as protective antigens.outer membranes were prepared by the sarkosyl method from 30 strains of pasteurella multocida and the closely related taxon 13, which had been isolated from cattle. the patterns of the outer membrane proteins (omps) on sds-page were generally similar to one another, though the four major proteins (a-d) varied somewhat in molecular mass; these patterns allowed the strains to be arranged into 12 groups. taxon 13 strains and typical p. multocida strains were indistinguishable, both types being foun ...19902342087
protection against progressive atrophic rhinitis by vaccination with pasteurella multocida toxin purified by monoclonal antibodies.pasteurella multocida toxin was purified by affinity chromatography and inactivated by treatment with formaldehyde before use as a single component vaccine against progressive atrophic rhinitis in pigs. twenty pregnant gilts which were vaccinated twice before farrowing with either low or high doses of the purified toxoid, developed dose-dependent positive serum and colostrum titres to the toxin and, unlike the progeny of 10 untreated control gilts, the offspring of the vaccinated gilts also had ...19892528856
pharmacokinetics of ceftazidime given alone and combination with probenecid to unweaned calves.ceftazidime pharmacokinetic values were studied in unweaned calves given the antibiotic alone or in combination with probenecid. ceftazidime was administered iv to 9 calves at a dosage of 10 mg/kg of body weight and im (10 mg/kg) to 8 calves, to 7 calves (10 mg/kg plus probenecid [40 mg/kg]), and to 9 calves (10 mg/kg plus probenecid [80 mg/kg]). serum concentration-vs-time data were analyzed, using noncompartmental methods based on statistical moment theory. the data for iv ceftazidime administ ...19892679252
cloning and expression of the pasteurella multocida toxin gene, toxa, in escherichia coli.a chromosomal dna library of a toxigenic type d strain of pasteurella multocida subsp. multocida was established in escherichia coli. from this library two clones, spe308 and spe312, were identified by using a monoclonal antibody against the osteoclast-stimulating p. multocida toxin (pmt). extracts of these clones showed cytopathic activity identical to that of extracts of toxigenic p. multocida. the recombinant plasmids, pspe308 and pspe312, directed the synthesis of a protein with an apparent ...19892680987
agarose soy casein digest medium for replacement of blood agar for potency determinations of live pasteurella vaccines.blood agar, prepared with trypticase (bbl microbiology systems, cockeysville, md.) soy agar and 5% defibrinated bovine blood, is used for testing the potency of live pasteurella multocida and pasteurella haemolytica vaccines, but its potential for variation makes it undesirable to use in a standard assay method. tests done with rpmi 1640 and trypticase soy medium indicated that the benefits obtained by adding defibrinated blood to the trypticase soy agar medium were more likely due to neutraliza ...19892705764
experimental pasteurella multocida pneumonia in calves.pasteurella multocida was isolated from the lungs of calves that died on a farm in the south of england. this organism was inoculated experimentally into 13 calves by the intratracheal route: in all but two of the calves mild clinical disease resulted and at necropsy, three or four days later, pneumonic consolidation involving up to 22 per cent of the lung was observed. p multocida was isolated from all but two of the lungs. of two calves inoculated intravenously with p multocida, one showed mil ...19892799074
pharmacokinetics of single doses of cefoxitin given by the intravenous and intramuscular routes to unweaned calves.cefoxitin pharmacokinetics and bioavailability were studied in unweaned calves. the antibiotic was administered to nine calves intravenously (i.v.), to seven calves intramuscularly (i.m.) at 20 mg/kg and to eight calves i.m. at 20 mg/kg together with probenecid at 40 mg/kg. serum concentration versus time data were analysed using statistical moment theory (smt). the i.v. data were also fitted by a linear, open two-compartment model. the elimination half-life (t1/2) was 66.9 +/- 6.9 min (mean +/- ...19883054149
prevention of experimental haemorrhagic septicaemia with a live vaccine.pasteurella multocida serotype b:3,4 isolated from a fallow deer in england was used as a vaccine to prevent haemorrhagic septicaemia. the deer strain was less virulent for calves than typical serotype b:2 of haemorrhagic septicaemia strains. it elicited antibodies in cattle that protected mice against serotype b:2 infection. the live deer vaccine containing 2 x 10(7) viable organisms per dose was used to immunise calves. six months after vaccination, five of six calves were protected against se ...19873111071
rabbit pasteurellosis: induced disease and vaccination.pasteurellosis was induced in rabbits by conjunctival inoculation with 2 strains of pasteurella multocida. the ld50 of strain p1062 (a bovine isolate) was 10(5.1) colony-forming units and that of strain p1059 (a turkey isolate) was 10(5.5) colony-forming units. pasteurella-free rabbits were vaccinated iv or mucosally with boiled cells of p multocida or a cross-reactive uridine diphosphogalactose epimerase-deficient mutant of escherichia coli j5. in rabbits challenge exposed with p multocida stra ...19883282457
clinical pharmacokinetics of five oral cephalosporins in calves.the minimal inhibitory concentrations (mic) of cephalexin, cephradine, cefaclor, cefatrizine and cefadroxil for salmonella species, escherichia coli and pasteurella multocida isolated previously from young calves were determined. the mic90 values for cephalexin, cephradine and cefadroxil ranged between 3.12 micrograms ml-1 and 12.5 micrograms ml-1, whereas those of cefatrizine and cefaclor were 3.12 micrograms ml-1 and 0.78 microgram ml-1, respectively. each drug was administered intravenously a ...19873317582
clinical pharmacokinetics of flumequine in calves.the minimal inhibitory concentration (mic) of flumequine for 249 salmonella, 126 escherichia coli, and 22 pasteurella multocida isolates recovered from clinical cases of neonatal calf diarrhoea, pneumonia and sudden death was less than or equal to 0.78 microgram/ml. the pharmacokinetics of flumequine in calves was investigated after intravenous (i.v.), intramuscular (i.m.) and oral administration. the two-compartment open model was used for the analysis of serum drug concentrations measured afte ...19863522937
properties of dermonecrotic toxin prepared from sonic extracts bordetella bronchiseptica.a toxin with dermonecrotic activity (dnt) was purified from sonic extracts of bordetella bronchiseptica l3 of pig origin at phase i by chromatographic and electrophoretic methods. the purification procedure was one developed for obtaining the pasteurella multocida dnt from sonic extracts with some modifications. dermonecrotizing activity of b. bronchiseptica-purified dnt was increased by 600-fold compared with that of the crude extract, and the average yield was about 3%. the toxin was homogeneo ...19863699886
characterization and identification of bovine and ovine pasteurellaceae isolated from the oral cavity and rumen of apparently normal cattle and sheep.reinvestigations of 121 strains previously described as actinobacillus lignieresii showed that only fifteen strains (12%) had phenotypic characters compatible with a. lignieresii sensu stricto. four strains were diagnosed as pasteurella multocida and nine strains as p. haemolytica biogroup 1. the remaining organisms had phenotypic characters compatible with pasteurellaceae, but could not be classified with accepted species. fifty-one of the strains investigated had phenotypic characters allowing ...19863728024
partial purification of an osteolytic toxin from pasteurella multocida.a protein toxin apparently composed of one polypeptide with an estimated mr of 155,000 was purified from sonicated cells of a type d strain of pasteurella multocida (lfb3) by preparative polyacrylamide gel electrophoresis (page) and deae-sephadex a50 chromatography. its specific activity was 150-fold greater than that of the crude extract. the partially purified protein was cytotoxic for embryonic bovine lung cells, lethal for mice and caused turbinate atrophy in gnotobiotic pigs; a single intra ...19863760819
preliminary studies with a live streptomycin-dependent pasteurella multocida and pasteurella haemolytica vaccine for the prevention of bovine pneumonic pasteurellosis.twelve pasteurella-free holstein-friesian calves were used in a study to test the efficacy of a live streptomycin-dependent pasteurella multocida a:3 and streptomycin-dependent pasteurella haemolytica a1 vaccine. the calves were inoculated intramuscularly twice at 14-day intervals with either the streptomycin-dependent vaccine, containing 1 x 10(6) colony forming units/ml p. multocida and 4 x 10(8) colony forming units/ml p. haemolytica, commercial bacterin, or phosphate buffered saline. two wee ...19853907804
clinical pharmacology of apramycin in calves.the minimal inhibitory concentrations (mic) of apramycin, a unique aminocyclitol antibiotic, were compared with the mic of dihydrostreptomycin and neomycin for 323 salmonella, 178 escherichia coli and twenty-six pasteurella multocida isolates recovered from newborn calves. apramycin exhibited better in vitro anti-bacterial activity than dihydrostreptomycin and neomycin; isolates of salmonella group b and e. coli resistant to the latter were sensitive to apramycin. the two-compartment open model ...19853989905
experimental hemorrhagic septicemia: gross and microscopic lesions resulting from acute infections and from endotoxin administration.gross and microscopic lesions observed in bovine and porcine hemorrhagic septicemia, and in bovine pasteurella multocida endotoxemia are described. widely distributed hemorrhages, edema, and general hyperemia were the most obvious tissue changes observed in the infected calves. pneumonia was a constant lesion. aerosol exposure produced a multiple focal fibrinosuppurative pneumonia, while intranasal and intramuscular inoculation resulted in generalized interstitial pneumonia. a slight lymphadenit ...19674227916
isolation from pasteurella multocida of a lipopolysaccharide antigen with immunizing and toxic properties.a heat-stable, particulate, lipopolysaccharide-protein antigenic complex has been isolated from a virulent, encapsulated strain of pasteurella multocida by extraction with cold, formalinized saline, and centrifugation at 105,000 x g. the original bacterial culture had been obtained from a bison that died of hemorrhagic septicemia, an infectious disease of cattle and buffalo. injection of fractional milligram amounts of the antigen into mice, rabbits, and calves produced toxic reactions which fre ...19674960160
studies on the occurrence of pasteurella multocida in the nasopharynx of healthy cattle. 19685690240
[serological typing of the somatic antigens of pasteurella multocida strains isolated from poultry and mammals].somatic antigens were used for the serotyping of a total of 80 pasteurella multocida strains isolated from birds with acute and chronic form of cholera as well as from mammals with bronchopneumonia. the investigations made use of the agglutination reaction between factorial sera (0:1, 0:2, 0:3, 0:5, 0:6, 0:8, 0:9, and 0:11) and antigens treated with 1 n hcl. the o-factorial sera behaved specifically after absorption with the respective reference antigens. they showed titers of 1:640 or higher th ...19846204437
protection of chickens by ribosomal vaccines from pasteurella multocida: dependence on homologous lipopolysaccharide.chickens were protected against fowl cholera by ribosomal vaccines prepared from noncapsulated pasteurella multocida. passive hemagglutination (pha) titers to lipopolysaccharide (lps) and the degree of protection conferred by ribosomal vaccines were diminished or abolished when ribosomes were chromatographed on an immunoadsorbent column. addition of subimmunogenic amounts of serotype 1 (homologous) lps to highly purified ribosomes resulted in vaccines that protected against challenge exposure an ...19846208828
nonspecific suppressive effect of bovine herpesvirus type 1 on bovine leukocyte functions.the effect of bovine herpesvirus type 1 on the specific and nonspecific immune response of calves was examined. animals with or without prior aerosol exposure to pasteurella haemolytica serotype a1 were aerosol challenged with 10(8) pfu of virus. blood and serum samples were taken before and after virus challenge for determining cell-mediated, humoral, and neutrophil responses. a significant depression of the blastogenic responses to phytohemagglutinin, p. haemolytica, and pasteurella multocida ...19836311742
effect of aztreonam on the growth of pasteurella multocida in the lung.specific-pathogen-free icr mice were infected aerogenically with pasteurella multocida and, beginning 1 hour later, were treated with aztreonam (50 mg/kg of body weight). the number of viable bacilli in the lungs, liver, and spleen were determined at intervals for up to 36 hours. aztreonam was bactericidal for growing bacilli in vitro and, when injected 1 and 5 hours after aerogenic exposure, provided greater than 80% protection after dosage at the level of 12.5 mg/kg. below this dosage level, v ...19846375488
[biochemical tests for identifying pasteurella multocida].studied was the biochemical activity of a total of 168 strains of pasteurella--73 isolated from birds (48 from cases of acute fowl cholera, and 25--of chronic cholera), and 95 isolated from mammals (3 from lambs, 24 from pigs, 36 from cattle, and 32 from rabbits) with regard to the tests determining the hemolytic activity, production of indol, reduction of nitrates, breakdown of urea, beta galactosidase activity, production of hydrogen sulfide, ornitin-, arginine-, lysine-decarboxylase-, and pho ...19846528479
effect of type a pasteurella multocida fractions on bovine polymorphonuclear leukocyte functions.the effect of various pasteurella multocida fractions on bovine polymorphonuclear leukocyte (pmn) functions was examined in vitro by using two encapsulated strains, p-2383 and p-1062 (both are carter capsular type a and of bovine origin). the ability of pmns to ingest staphylococcus aureus and iodinate protein was significantly inhibited in the presence of live cells, heat-killed whole cells, or saline-extracted capsules but not in the presence of the decapsulated heat-killed cells. none of the ...19846690417
biochemical and serological studies of pasteurella multocida isolated from cattle and buffaloes in malaysia. 19816793206
cross-reactivity of haemophilus somnus antibody in agglutination and complement fixation tests and in the enzyme-linked immunosorbent assay.the specificity and sensitivity of agglutination, complement fixation, and enzyme-linked immunosorbent assay (elisa) procedures in the detection of antibodies to haemophilus somnus was investigated. h. somnus rabbit immune sera were found to agglutinate pasteurella multocida, staphylococcus aureus, and haemophilus agni and, in some instances, also pasteurella haemolytica, salmonella dublin, streptococcus agalactiae, and corynebacterium pyogenes. in complement fixation tests with saline extracts ...19836841584
[intrauterine bacterial and mycotic infections in cows].bacteriologic, mycologic, and serologic investigations of cows and calves and three experiments with rabbits were carried out to shed light on the bacteriology and mycology of the intrauterine infections in cows. the following organisms were isolated from the investigated cows that exhibited disturbances in their reproduction, and had abortions, or gave birth to calves that were affected with diseases or died: escherichia coli (17.84%), association of bacteria (7.64%); vibrio genitalis (3.86%); ...19836880018
[further characterization of lipopolysaccharides from pasteurella multocida].the biological properties, sedimentation behaviour, and electron-optically visualised structures of lipopolysaccharides from pasteurella multocida were found to be identical with lipopolysaccharides from enterobacteriaceae. hard evidence to the effect that lipopolysaccharides from pasteurella multocida are r-lipopolysaccharides were produced by the composition of monosaccharides together with experiments on the basis of disc electrophoresis.19817247623
[identification of pasteurella serotypes by indirect hemagglutination].the indirect haemagglutination test was used for serological type identification of pasteurella strains. the pasteurella multocida strains were tested by parallel application of antigen production methods (common method plus hyaluronidase treatment). thirty-nine (72.2 per cent) of 54 pasteurella multocida strains, which had been obtained from cattle and swine, were serologically tested by the common technique of antigen production, their types being identified, while 42 (77.7 per cent) were test ...19817283648
[determination of the nucleotide composition of pasteurella multocida strains].studied was spectrophotometrically the nucleotide composition of dna of pasteurella multocida strains along with studies on the nucleotide base ratio, the molar relations between nucleotide bases, the guanine/cytosine ratio in percent, and the specificity index adenine-tinin/guanine cytosine. the strains used were isolated from birds with various forms of fowl cholera (acute, atypic, and swelling of the wattles), and from calves with bronchopneumonia. used were also mutants that were streptomyci ...19817340107
orally administered tylosin for the control of pneumonia in neonatal calves.the effectiveness of orally administered tylosin tartrate for the control of naturally occurring pneumonia was determined in 287 neonatal calves. tylosin tartrate was mixed with reconstituted milk replacer at the time of feeding. daily doses of 1.0 g (0.5 g bid), 2.0 g (1.0 g bid) and 4.0 g (2.0 g bid) were evaluated for periods ranging from seven to 28 days. tylosin at the optimum dose of 2.0 g daily reduced mortality to 12 out of 95 (12.6 per cent) compared to 38 out of 89 (42.7 per cent) in t ...19807445393
proposed quality control guidelines for antimicrobial susceptibility tests using tilmicosin.quality control guidelines for tilmicosin, a novel veterinary-use-only macrolide, were developed in a multi-laboratory study according to established national committee for clinical laboratory standards (nccls) procedures (m23-t2). tilmicosin was incorporated into sensititre plates for broth microdilution endpoint testing and into two lots of 15-micrograms disks for kirby-bauer agar disk diffusion testing. one common lot and five unique lots of mueller-hinton media were used. (broth was cation a ...19957714188
transferrin receptors on ruminant pathogens vary in their interaction with the c-lobe and n-lobe of ruminant transferrins.the interaction between ruminant transferrins and receptor proteins on the surface of the ruminant pathogens pasteurella haemolytica, haemophilus somnus, pasteurella multocida, haemophilus agnii, and moraxella bovis was evaluated by a combination of binding assays and affinity isolation procedures. membranes isolated from p. haemolytica, p. multocida, and h. agnii were capable of binding sheep, goat, and cattle transferrins whereas binding by membranes from h. somnus and m. bovis was specific fo ...19948076248
morbidity and mortality in a large irish feedlot; microbiological and serological findings in cattle with acute respiratory disease.a survey of morbidity and mortality was undertaken in a slatted unit containing 6399 beef cattle over a 6 month period. the mortality rate was 0.78% and the morbidity rate was 12.7%. the interval from arrival to morbidity was 25.5 days +/- 0.9. significantly more sick cattle were identified on either mondays or tuesdays than were seen on saturdays or sundays (p < 0.005). the mean interval to mortality (all diseases) was 48.5 days +/- 7.4. respiratory disease was the most frequently recorded dise ...19938111615
immunogens of pasteurella.the family pasteurellaceae pohl contains gram-negative, facultatively anaerobic and fermentative bacteria of the genera pasteurella, haemophilus, and actinobacillus. approximately 20 different species of the genus pasteurella have been identified using phenotypic and genetic analyses. of these species, p. multocida and p. haemolytica are the most prominent pathogens in domestic animals causing severe diseases and major economic losses in the cattle, swine, sheep, and poultry industries. mechanis ...19938116191
pasteurella multocida: oropharyngeal carriage and antibody response in breeders.the presence of pasteurella multocida in the oropharynx of 58 pig, cattle or rabbit breeders whose livestock had suffered from pasteurellosis was investigated using a selective medium. blood samples for serological studies were collected at the same moment. nineteen breeders were found to host one p multocida subsp multocida strain. oropharyngeal carriage of p multocida was found to be more frequent in pig breeders (42% of individuals) than in cattle (10%) or rabbit (0%) breeders. genomic polymo ...19948142961
toxin production by pasteurella multocida isolated from rabbits with atrophic rhinitis.naturally acquired turbinate atrophy in rabbits was associated with pasteurella multocida infection. several in vitro and in vivo studies were conducted to document toxin production from p multocida isolates and to determine the relation of toxin to atrophic rhinitis in rabbits. ten isolates of p multocida serotype a:12 were obtained from adult new zealand white rabbits with noninduced atrophic rhinitis. specific-pathogen-free rabbits inoculated intranasally with isolates of p multocida develope ...19938214896
[the effect of toxins from pasteurella multocida type d in calves in vivo].by intratracheal injection of purified dermonecrotic toxin from a pasteurella multocida type d strain, pneumonitis in calves could be produced. this demonstrates the important role of this toxin in the pathogenesis of pneumonitis in calves.19938471015
evaluation of the rapid nh system for identification of haemophilus somnus, pasteurella multocida, pasteurella haemolytica, and actinobacillus pleuropneumoniae isolated from cattle and pigs with respiratory disease.haemophilus somnus, pasteurella haemolytica, pasteurella multocida, and actinobacillus pleuropneumoniae from cattle and pigs with respiratory disease were used to evaluate the rapid nh system (innovative diagnostics, atlanta, ga.). minor modifications of the rapid nh system to include animal source and growth requirements would permit the identification of all isolates tested.19938501242
isolation of a spermatozoa motility inhibiting factor from chicken seminal plasma with antibacterial property.a 78-kda spermatozoa motility inhibiting factor (smif) was purified from chicken (gallus domesticus) seminal plasma by anion exchange (de-53) followed by affinity chromatography on concanavalin a-sepharose. the factor is thermostable and inhibited the spermatozoa motility in a dose dependent manner. in addition, smif inhibited the growth of gram negative bacteria, pasteurella multocida but not gram positive streptococcus equi. the factor lost its spermatozoa immobilizing property after treatment ...19958541320
pasteurella multocida toxin stimulates mitogenesis and cytoskeleton reorganization in swiss 3t3 fibroblasts.pasteurella multocida toxin (pmt) causes cytoplasmic retraction in epithelial cells, activates osteoclast neoformation, and is a potent mitogen for swiss 3t3 fibroblasts. in the present study designed to further investigate the effects of pmt on cell shape and proliferation, we report that the mitogenic effect of affinitypurified pmt on quiescent 3t3 cells was even superior at 5 ng/ml to that of fetal bovine serum or bombesin. this positive effect was inhibited by heat denaturation and methylami ...19968647912
epidemiologic and pathologic characteristics of respiratory tract disease in dairy heifers during the first three months of life.to describe the incidence of respiratory tract disease in dairy calves and to compare antibody titers and microbial isolates from transtracheal wash samples between calves with and without respiratory tract disease (cases and controls, respectively).19968707680
influence of chondroitinase on indirect hemagglutination titers and phagocytosis of pasteurella multocida serogroups a, d and f.capsules of pasteurella multocida serogroups a, d and f contain mucopolysaccharides which block antigenic determinants and prevent phagocytosis. in this study, capsules of serogroup a, d and f strains of p. multocida were depolymerized by enzyme treatment. capsule depolymerization of serogroup d and f strains with chondroitinase increased indirect hemagglutination (iha) test titers and enhanced phagocytosis by swine neutrophils. capsule depolymerization of serogroup a strains with hyaluronidase ...19958748544
the fractional inhibitory concentration index of antimicrobial agents for bacteria and mycoplasma isolated from the nasal swabs of cattle with respiratory diseases.we investigated the effect of thiamphenicol plus lincomycin (tp + lcm) and thiamphenicol plus tylosin (tp + ts) combinations using checker board method on the growth of pasteurella (p.) multocida, p. haemolytica and mycoplasma (m.) bovis by calculating the fractional inhibitory concentration index (fic index). the results showed that the fic indexes of the tp + lcm combination for p. multocida, p. haemolytica and m. bovis were 0.36 +/- 0.10, 0.72 +/- 0.09 and 0.81 +/- 0.18, respectively. the fic ...19968877979
in-vitro activity of ceftiofur against australian isolates of the family pasteurellaceae associated with respiratory disease in cattle and pigs. 19968894010
antibody responses of cattle to outer membrane proteins of pasteurella multocida a:3.to quantify the serum antibody responses to pasteurella multocida a:3 outer membrane proteins (omp) for cattle vaccinated with the homologous serogroup and to correlate those responses with the extent of experimentally induced pneumonia.19968896683
validation of random amplified polymorphic dna assays by ribotyping as tools for epidemiological surveys of pasteurella from animals.two collections of strains of pasteurella were studied for epidemiological purposes by ribotyping and random amplified polymorphic dna (rapd) assays. these strains were isolated through two different structures of animal productions: cattle and rabbit. forty strains of p. haemolytica from cattle reared in independent breeding-herds belonged to only 3 ribotypes after digestion with hindiii and pvuii. no further discrimination of these strains was obtained by rapd assays. all these 40 strains show ...19968914254
interpretive criteria for antimicrobial susceptibility testing of ceftiofur against bacteria associated with swine respiratory disease.ceftiofur, an extended-spectrum cephalosporin, is active against a variety of animal pathogens, including organisms associated with swine respiratory disease. however, minimum inhibitory concentration (mic) breakpoint and disk diffusion interpretive criteria have not been established for swine pathogens. susceptibility tests were performed by broth microdilution mic and disk diffusion methods on 246 bacterial species that cause swine respiratory disease. ceftiofur was active against salmonella s ...19968953533
restricted b-cell responses to microbial challenge of the respiratory tract.the local b-cell response in the respiratory tract to infectious challenge has been analyzed in pigs and calves using two techniques: flow cytometry and antibody secreting cell (asc) probes. pneumonia in pigs caused by experimental infection with mycoplasma hyopneumoniae resulted in a 25-fold increase in the b-cell population in bal and lung parenchyma 28 days post infection. asc probes revealed that the b-cell response of immune pigs to a large challenge infection was localized to lung parenchy ...19968988866
repromicin derivatives with potent antibacterial activity against pasteurella multocida.reductive amination of repromicin with polyfunctional amines has led to new macrolide antibacterial agents, some of which are highly potent against the gram-negative pathogen pasteurella multocida both in vitro and in a mouse infection model. a key element in this discovery was the recognition that among certain known macrolides increasing lipophilicity results in diminished in vivo activity. one repromicin derivative, 20-[n-[3-(dimethylamino)-propyl]-n-l-alanylamino]-20-deoxorepro micin (35), w ...19979083494
protection against haemorrhagic septicaemia induced by vaccination of buffalo calves with an improved oil adjuvant vaccine.an experimental oil adjuvant vaccine was developed against haemorrhagic septicaemia, a disease of cattle and buffalo caused by pasteurella multocida serotype b and e. mineral oil, mercol 52, was used as adjuvant together with span 85 and tween 85 as emulsifiers. the vaccine was evaluated by single dose intramuscular immunisation of 1-2 year old buffalo calves. igg and igm class antibodies were determined by elisa. the group of animals immunised with the experimental oil adjuvant vaccine showed a ...19979351202
immunity provided by haemorrhagic septicaemia-subunit vaccine in ruminants.a subunit vaccine against haemorrhagic septicaemia (hs), the principal killer disease in ruminants, especially cattle and carabao, in the philippines, has been developed. using capsules of pasteurella multocida group b as an active component of the vaccine, it gave solid protection against challenge with live organism in mice. an active calf protection test showed that in 24 hours, animals vaccinated with plain saline died after challenge compared to those given the subunit vaccine which survive ...19989554285
tn5706, a transposon-like element from pasteurella multocida mediating tetracycline resistance.the 4,378-bp putative tetracycline resistance transposon tn5706 of pasteurella multocida consists of an internal tet(h)-tetr resistance gene region which is flanked by almost identical insertion elements, is1596 and is1597. two reading frames for proteins of 70 and 228 amino acids were detected in each of these insertion sequence elements. the 228-amino-acid protein revealed homology to transposase proteins of gram-positive bacteria.19989687418
occurrence of some viruses and bacteria involved in respiratory diseases of ruminants in hungary.serological surveys, virological and bacteriological investigations were carried out on large populations of lambs and calves kept in intensive fattening units on hungarian large-scale farms, in order to study the occurrence of viruses and bacteria involved in the respiratory disease complex. the investigations were carried out in stocks where 5-20% of the young animals succumbed to acute or chronic pneumonia every year. serological surveys revealed that seropositivity to bovine adenovirus type ...19989713141
comparison of radiographic and necropsy findings of lung lesions in calves after challenge exposure with pasteurella multocida.to test suitability of radiographic evaluation of lung lesions as a substitute for lung lesion scores derived by examination at necropsy in challenge-exposure models of bovine pneumonia.19989736385
intracellular accumulation, subcellular distribution, and efflux of tilmicosin in chicken phagocytes.tilmicosin is a semi-synthetic macrolide antibiotic, currently approved for veterinary use in cattle and swine respiratory disease, and is in development for use in poultry mycoplasma air sacculitis. in order to provide an understanding of clinical efficacy, the in vitro interaction of tilmicosin with three types of chicken phagocytes (mq-ncsu macrophages, monocyte-macrophages, and heterophils) was evaluated. after incubation with radiolabeled tilmicosin, uptake was determined and expressed as t ...19989776059
effects of the major pasteurella multocida porin on bovine neutrophils.to evaluate in vitro effect of the major fraction of outer membrane proteins of pasteurella multocida with porin-like activities on some biological functions of bovine neutrophils.19989781460
[capsule formation in pasteurella multocida, serovar a].in this work the process of capsule formation in p.multocida bovine strain, serovar a, has been studied. the cultures were grown on liquid and solid nutrient media prepared on the basis of hottinger hydrolysate and synthetic culture medium 199. extracellular material was detected by the method electron cytochemistry with ruthenium red and polycationic ferritin. as revealed by specific staining and labeling, p.multocida capsule of serovar a was found to contain material of the polysaccharide natu ...19989949492
[long-chain fatty acids of pasteurella multocida nad pasteurella haemolytica].the relative contents of long-chain fatty acids in p. multocida and p. haemolytica were investigated. a dependence on the composition of the broth was established. accordingly, comparative quantitative studies on fatty acid contents have to be conducted using bacteria grown with the same lot of broth medium. as for p. multocida, there were significant differences between the serovars (c14 in tdhm and c16, delta 2c18 in bpl). these differences are, however, not significant to replace serotyping. ...199710084940
effects of the lipopolysaccharide-protein complex and crude capsular antigens of pasteurella multocida serotype a on antibody responses and delayed type hypersensitivity responses in the chicken.the effects of the lipopolysaccharide-protein complex (lps) and crude capsular antigen (cca) prepared from pasteurella multocida serotype a isolated from a duck in the philippines, on antibody responses to sheep red blood cells (srbc) and brucella abortus (ba) and delayed type hypersensitivity (dth) responses to bovine serum albumin (bsa) in the chickens were studied. chickens injected subcutaneously with lps and cca at 1 and 2 weeks of age and immunized intravenously with the mixed antigens of ...199910379953
pharmacokinetics of enrofloxacin and danofloxacin in plasma, inflammatory exudate, and bronchial secretions of calves following subcutaneous administration.enrofloxacin (2.5 mg/kg of body weight) and danofloxacin (1.25 mg/kg) were administered subcutaneously to ruminating calves (n = 8) fitted with subcutaneous tissue cages. concentrations of enrofloxacin, its metabolite ciprofloxacin, and danofloxacin in blood (plasma), tissue cage exudate (following intracaveal injection of 0.3 ml of 1% [vol/wt] carrageenan), and bronchial secretions were measured by high-performance liquid chromatography (hplc) and microbiological assay (enrofloxacin plus ciprof ...199910428924
candidate vaccine antigens and genes in pasteurella multocida.pasteurella multocida is the causative agent of fowl cholera and other diseases of production animals. isolates are classified into five groups based on capsular antigens and into 16 serotypes based on lps antigens. strains causing fowl cholera are most frequently designated a:1, a:3 or a:4. whole cell bacterins can provide some degree of protection, but only against the homologous lps serotype. there is good evidence that cross-protective antigens are expressed only under in vivo conditions. em ...199910486918
restriction endonuclease analysis using hhal and hpall to discriminate among group b pasteurella multocida associated with haemorrhagic septicaemia.the purpose of this study was to improve and standardise restriction endonuclease analysis (rea) for discriminating isolates of serogroup b pasteurella multocida associated with haemorrhagic septicaemia in wild and domestic animals and to create a reference database that can be used for epidemiological studies. two techniques for extraction and isolation of chromosomal dna were compared, a dnazol method and an enzymic lysis followed by a two-phase partition method. no differences were observed b ...200010628829
pathological and microbiological studies on pneumonic lungs from danish calves.during 1 year, the association between microbiological and pathological findings in 72 lungs from calves submitted to the danish veterinary laboratory for diagnostic purposes was studied. all cases were evaluated pathologically and bacteriologically, whereas only 68 cases were examined for the presence of bovine respiratory syncytial virus (brsv), parainfluenza-3 virus (pi-3 virus) and bovine coronavirus, 62 cases for bovine viral diarrhoea virus (bvd), 45 cases for bovine adenovirus and 51 case ...199910676147
isolation of respiratory bovine coronavirus, other cytocidal viruses, and pasteurella spp from cattle involved in two natural outbreaks of shipping fever.to identify cytocidal viruses and pasteurella spp that could be isolated from cattle involved in 2 natural outbreaks of shipping fever.200010825949
induction of parturition with prostaglandin f2 alpha as a possible model to study impaired reproductive performance in the dairy cow.parturitions were induced in five cows, 2 weeks before term using prostaglandin (pg) f(2alpha). two i.m. injections were performed with an interval of 24 h. all cows calved within 5 days (average 2.7 days) after the first injection of pgf(2alpha). out of five cows, four had retained fetal membranes (rfm). each animal was sampled for bacteriological examination using uterine biopsies twice a week during 42 days postpartum (pp). jugular vein blood samples were withdrawn for pgf(2alpha)-metabolite ...200010837974
evaluation of the measurement of leukotriene b4 concentrations in exhaled condensate as a noninvasive method for assessing mediators of inflammation in the lungs of calves.to determine whether measurement of an inflammatory mediator in exhaled condensate could provide a noninvasive method for evaluating lungs of calves.200010895893
genetic organization of pasteurella multocida cap loci and development of a multiplex capsular pcr typing system.current serotyping methods classify pasteurella multocida into five capsular serogroups (serogroups a, b, d, e, and f) and 16 somatic serotypes (serotypes 1 to 16). in the present study, we have developed a multiplex pcr assay as a rapid alternative to the conventional capsular serotyping system. the serogroup-specific primers used in this assay were designed following identification, sequence determination, and analysis of the capsular biosynthetic loci of each capsular serogroup. the entire ca ...200111230405
interactions between bovine endothelial cells and pasteurella multocida: association and invasion.we investigated the association and the invasion of a bovine aortic endothelial cell (baec) line by pasteurella multocida to study the potential role of internalized bacteria and possible intracellular survival during pasteurella infections. our data indicate that p. multocida is able to adhere to and to invade baecs. the density of the bacterial population plays a defined role for an optimal mechanism of interaction between bacteria and cells, as does the incubation period of association and in ...200111281326
use of polymerase chain reaction to identify brucella abortus strain rb51 among brucella field isolates from cattle in italy.brucella abortus strain rb51, a rough mutant of the b. abortus 2308 virulent strain, was recently approved in the united states as the official vaccine for brucellosis in cattle. following recent evidence of unauthorized use of rb51 vaccine in italy, where the use of vaccines for brucellosis is no longer allowed, the suitability of an rb51-specific polymerase chain reaction assay for identifying the rb51 strain among brucella field isolates from cattle in italy was investigated. the oligonucleot ...200111315520
specific antibodies of pasteurella multocida in newborn calves of vaccinated dams.twenty-five newborn holstein friesian calves, from dams vaccinated against haemorrhagic septicaemia (hs), were tested repeatedly over the first 6 months of life to monitor the transferred antibody levels against hs. enzyme-linked immunosorbent assays were used to measure the specific hs antibodies with antigens from pasteurella multocida strains b:2 and e:2. there was a significant curvilinear relationship between the monitored igg response and the age of the calves. peak serum igg levels were o ...200111474861
random amplification of polymorphic dna and phenotypic typing of zimbabwean isolates of pasteurella multocida.eighty-one isolates presumptively identified as pasteurella multocida from a variety of diseases in animals in zimbabwe were subjected to biochemical characterization, capsular typing and rapd analysis. the majority of isolates (over 80%) were assigned into named taxa and were predominantly p. multocida subsp. multocida and p. multocida subsp. septica, whilst the remainder were unassigned. serogroup a was predominant among the three capsular types (a, b and d) of p. multocida detected. three mai ...200111506929
antimicrobial susceptibility of pasteurella multocida isolated from cattle and pigs.minimum inhibitory concentrations (mics) of 10 antimicrobial agents were determined for pasteurella multocida from cattle and pigs (72 and 68 isolates, respectively). higher mics were observed with oxytetracycline, doxycycline, tilmicosin and thiamphenicol for porcine isolates than for bovine isolates. enrofloxacin was the most active, with an mic for 90% of the isolates (mic90) of 0.05 microg/ml for both bovine and porcine isolates. aspoxicillin exhibited the same excellent activity against pen ...200111666039
biofilm bacteria: formation and comparative susceptibility to antibiotics.the calgary biofilm device (cbd) was used to form bacterial biofilms of selected veterinary gram-negative and gram-positive pathogenic bacteria from cattle, sheep, pigs, chicken, and turkeys. the minimum inhibitory concentration (mic) and minimum biofilm eradication concentration (mbec) of ampicillin, ceftiofur, cloxacillin, oxytetracycline, penicillin g, streptomycin, tetracycline, enrofloxacin, erythromycin, gentamicin, tilmicosin, and trimethoprim-sulfadoxine for gram-positive and -negative b ...200211989739
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