Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| association of bola drb3 and dqa1 alleles with susceptibility to neospora caninum and reproductive outcome in quebec holstein cattle. | the bola drb3 and dqa1 genes are part of the major histocompatibility complex (mhc) class ii in cattle. these genes are highly polymorphic and have been associated with resistance to several diseases, such as mastitis, bovine leukemia virus (blv) and dermatophilis. sequenced based typing of these genes has been carried out extensively from blood samples; however it is often impractical or expensive to obtain such samples. repositories of well-characterized serum from cattle are readily available ... | 2009 | 19646818 |
| microsatellite markers for the molecular characterization of neospora caninum: application to clinical samples. | we report here on the development of a nested pcr procedure for the application of neospora caninum microsatellite markers to clinical samples. genotyping technology by fluorescently labelled dna fragment analysis was used in combination with dna sequencing for those markers which show additional snps or complex repetitive sequences. twenty-nine dna samples from naturally infected bovine aborted foetuses from two regions of spain where n. caninum had been detected by nested pcr of the its1 rrna ... | 2009 | 19720464 |
| dynamics of heat shock protein 70 concentrations in peripheral blood lymphocyte lysates during pregnancy in lactating holstein-friesian cows. | the aim of this study was to characterize the dynamics of the concentrations of heat shock protein 70 kda (hsp70) in peripheral blood lymphocytes of lactating holstein-friesian dairy cows (bos taurus) during pregnancy. the detection of pregnancy was carried out and blood samples collected on days 40, 90, 120, 150, 180, and 210 of gestation from 46 cows (11 primiparous and 35 pluriparous, 34 seropositive and 12 seronegative to neospora caninum). peripheral blood lymphocytes were isolated by densi ... | 2009 | 19729195 |
| [preliminary application of pcr-based assay for the detection of neospora caninum in bovine aborted fetus]. | to establish a pcr diagnostic method based on nc-5 gene of neospora caninum, for being used to detect neospora in brain tissues of bovine aborted fetus. | 2009 | 19856504 |
| diagnosis of neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-pcr. | neospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-pcr, using primers from the nc5 region of the genomic dna (pcr plus) and primers from the its1 region of the ribosomal dna (pcr jb). a total of 105 fetal samples sent to the centro de pesquisa e desenvolvimento de sanidade animal do instituto biológico from january 2006 to may 2008 were examined for evidence of n. caninum. histological examination revealed 71.4% with non-sup ... | 2009 | 20040203 |
| comparison between immunohistochemistry and two pcr methods for detection of neospora caninum in formalin-fixed and paraffin-embedded brain tissue of bovine fetuses. | the objective of this study was to identify the presence of the parasite by comparing immunohistochemistry (ihc) with two polymerase chain reaction (pcr) methods for the detection of the pnc5 gene and the internal transcribed spacer 1 (its1) of n. caninum in brain tissue of bovine fetuses that had previously been fixed in formalin and paraffin-embedded. in 29 out of 48 brains (60.4%), microscopic lesions consistent with neospora infection were observed, and 21 of the 29 cases (72.41%) were posit ... | 2009 | 19515495 |
| genetic diversity amongst isolates of neospora caninum, and the development of a multiplex assay for the detection of distinct strains. | infection with neospora caninum is regarded as a significant cause of abortion in cattle. despite the economic impact of this infection, relatively little is known about the biology of this parasite. in this study, mini and microsatellite dnas were detected in the genome of n. caninum and eight loci were identified that each contained repetitive dna which was polymorphic among different isolates of this parasite. a multiplex pcr assay was developed for the detection of genetic variation within n ... | 2009 | 19496247 |
| neospora caninum--how close are we to development of an efficacious vaccine that prevents abortion in cattle? | neospora caninum is a protozoan parasite that causes abortion in cattle around the world. although the clinical signs of disease in both dogs and cattle have now been recognised for over 20years, treatment and control options are still limited, despite the availability of a commercial vaccine in some countries of the world. the case for an efficacious vaccine has not been convincingly waged by farmers, veterinarians and other members of the agricultural and rural communities. in recent times, ho ... | 2009 | 19497326 |
| [use of the real time rt-pcr for immune related gene expression quantitation in experimentally infected neospora caninum bovine calves]. | neospora caninum is one of the main causes of abortion and natimortality in cattle. host immune defense is capable to inhibit tachyzoite activity during acute infection, but there is no action against bradyzoites in tissue cysts. activation and modulation of this response is controlled by cell mediators. the real-time rt-pcr technique was employed to detect some of those mediators during n. caninum infection. holstein and nelore calves intramuscularly infected with tachyzoites and uninfected con ... | 2009 | 19500454 |
| stage-specific expression of nc sag4 as a marker of chronic neospora caninum infection in a mouse model. | neospora caninum infection persists throughout the life of its intermediate host due to the conversion of tachyzoites to slowly dividing bradyzoites that encyst in the brain. this event results in persistent n. caninum infection in bovine herds and partially explains the poor efficacy of many chemotherapeutic agents and vaccine formulations. thus, there is a need for greater understanding of the tachyzoite-to-bradyzoite conversion mechanisms. here we studied for the first time the transcription ... | 2009 | 19402941 |
| effects of crossbreed pregnancies on the abortion risk of neospora caninum-infected dairy cows. | previous studies have shown that the use of beef bull semen significantly reduces the rate of abortions due to neospora caninum in artificially inseminated (ai) seropositive dairy cows. in addition, certain beef breeds could be more resistant to n. caninum infection and abortion than others. the aim of the present study was to determine whether different crossbreed pregnancies, those derived from limousin, charolais, piedmontese or belgian blue semen, carry different risks of abortion in neospor ... | 2009 | 19464119 |
| neospora caninum and complex vertebral malformation as possible causes of bovine fetal mummification. | bovine neosporosis, caused by neospora caninum is a leading cause of abortion in cattle. we postulated that neosporosis could lead to fetal death and mummification. fifteen mummified fetuses were tested by polymerase chain reaction (pcr) for the mutation in the bovine slc35a3 gene that causes complex vertebral malformation (cvm) and the pnc-5 gene which identifies n. caninum infection. dna was extracted from the mummified fetuses and the sex of the mummies was determined by pcr. the cvm mutation ... | 2009 | 19436446 |
| short report: prevalence of neospora caninum and toxoplasma gondii antibodies in northern egypt. | in view of the worldwide importance of toxoplasma gondii and neospora caninum and the limited data on the seroprevalence of these parasites in egypt, this study aimed to estimate the prevalence of anti-t. gondii and anti-n. caninum antibodies in rabbits, cattle, and humans. we used elisa methods based on surface antigen 2 of t. gondii (tgsag2t) and surface antigen 1 of n. caninum (ncsag1t). high seroprevalence of t. gondii (51.49%) was detected in pregnant women, and antibodies to n. caninum wer ... | 2009 | 19190225 |
| specific anti-neospora caninum igg1 and igg2 antibody responses during gestation in naturally infected cattle and their relationship with gamma interferon production. | gamma interferon (ifn-gamma) production has been attributed a protective role against abortion in neospora-infected dairy cows. this study investigate possible relationships between plasma levels of the n. caninum-specific immunoglobulin (ig) isotypes igg1 and igg2 and ifn-gamma production throughout gestation in dairy cows naturally infected with the parasite. data were obtained from 31 pregnant neospora-seropositive animals. blood samples were collected on gestation days 40, 90, 120, 150, 180 ... | 2009 | 19201036 |
| risk factors associated with neospora caninum infections in cattle in argentina. | from 2003 through 2007, serum samples from 5594 dairy and beef heifers and cows in argentina were assessed to quantify the association between presence of neospora caninum antibodies and history of abortion, type of exploitation, and age category of animals. animals with a history of abortion were 85% more likely (p<0.01) to be positive to n. caninum than animals without a record of abortion. for a given category (age) of animals, being in a dairy operation increased the odds of being n. caninum ... | 2009 | 19216028 |
| neospora caninum in dairy herds in schleswig-holstein, germany. | the aim of a cross-sectional survey performed between november 2005 and april 2006 was to obtain actual information on the occurrence of neospora (n.) caninum infection in dairy cows of schleswig-holstein. it was planned to examine tank-milk of about 35% of the approximately 6,000 dairy herds in schleswig-holstein for n. caninum-specific antibodies using a p38-tank-milk elisa. this test is able to detect a within-herd n. caninum-seroprevalence of > 10%. only 20 (1.0%) out of 1,950 tank-milk samp ... | 2009 | 19226934 |
| [inquiry of antibodies anti-neospora caninum in dairy cattle, dogs and rural workers of the south-west region of mato grosso state]. | considering the importance of neosporosis in the animal health and production, the frequency of antibodies to neospora caninum was evaluated in dairy cattle of the southwestern region of mato grosso state, brazil, in addition to serum samples obtained from dogs and humans living in the farms. a total of 1036 serum samples were analyzed, from which 932 were from dairy bovine females, 37 from dogs and 67 from humans, from 24 farms and examined by the indirect fluorescent antibody test (ifat). reac ... | 2009 | 20040187 |
| detection of neospora caninum in aborted bovine fetuses and dam blood samples by nested pcr and elisa and seroprevalence in beijing and tianjin, china. | neospora caninum infection is a significant cause of abortion in cattle. we investigated the tissue distribution of n. caninum in aborted bovine fetuses and dam blood samples by a nested pcr assay, and compared the nested pcr with elisa in the diagnosis of n. caninum infection. in total, 26 aborted fetuses and 813 blood samples were collected from 8 dairy herds in beijing (n=212) and tianjin (n=601), china. fifteen fetuses (57.7%) were tested n. caninum-positive by the nested pcr. n. caninum dna ... | 2009 | 19660160 |
| seroprevalence of neospora caninum and coexistence with toxoplasma gondii in dogs. | neospora caninum is a protozoal agent causing abortion and infertility problems in dairy cattle. the objective of the present study was to detect the seroprevalence of n.caninum in dogs and its co-existence with toxoplasma gondii in some rural and urban regions of kirikka-le province. a total of 121 blood samples were examined with indirect immunoflorescent antibody test to detect the presence of n.caninum igg antibodies. the seropositivity of n.caninum was 28.9% in dogs. the seropositivity rate ... | 2009 | 19598086 |
| indoleamine 2,3-dioxygenase is involved in defense against neospora caninum in human and bovine cells. | neospora caninum is an apicomplexan parasite closely related to toxoplasma gondii. in nature this parasite is found especially in dogs and cattle, but it may also infect other livestock. the growth of n. caninum, which is an obligate intracellular parasite, is controlled mainly by the cell-mediated immune response. during infection the cytokine gamma interferon (ifn-gamma) plays a prominent role in regulating the growth of n. caninum in natural and experimental disease. the present study showed ... | 2009 | 19620347 |
| myeloid differentiation factor 88 is required for resistance to neospora caninum infection. | neospora caninum is an intracellular parasite that causes major economic impact on cattle raising farms, and infects a wide range of warm-blooded hosts worldwide. innate immune mechanisms that lead to protection against this parasite are still unknown. in order to investigate whether myeloid differentiation factor 88 (myd88) is required for resistance against n. caninum, genetically deficient mice (myd88(-/-)) and wild type littermates were infected with live tachyzoites and the resistance to in ... | 2009 | 19341611 |
| the first finding of neospora caninum and the occurrence of other abortifacient agents in sheep in slovakia. | neosporosis is an infection of animals caused by an intracellular coccidian parasite, neospora caninum, closely related to toxoplasma gondii. the parasite is one of important abortifacient agents of bovine abortions worldwide. the aim of the study was to detect the prevalence of anti-neospora antibodies in dairy aborting sheep from two eastern slovak regions and to compare it with the occurrence of other potential abortifacient agents. sera of 382 sheep, mainly the improved valachian and merino ... | 2009 | 19540672 |
| seroprevalence of neospora caninum in dairy cattle ranches with high abortion rate: special emphasis to serologic co-existence with toxoplasma gondii, brucella abortus and listeria monocytogenes. | the aim of this study was to determine seroprevalence of neospora caninum in dairy cattle ranches with higher abortion rates and co-existence of neospora caninum, toxoplasma gondii, brucella abortus, and listeria monocytogenes antibodies. the blood samples were collected from dairy cows with history of abortion (n=234) as well as from pregnant cows that were (n=323) kept in the same ranches. n. caninum seroprevalence was 10.77% (60/557). the co-existence rate of n. caninum seropositivity with t. ... | 2009 | 19592171 |
| isolation and characterization of a bovine isolate of neospora caninum with low virulence. | neospora caninum tachyzoites were isolated from the brain of an asymptomatic naturally infected calf with precolostral-specific antibodies. the new isolate, named nc-spain 1h, was identified as a member of the n. caninum species based on its internal transcribed spacer 1 (its-1) sequence and was genetically characterized using microsatellite markers. multilocus analysis showed that nc-spain 1h was genetically different from other n. caninum isolates. we compared the in vitro tachyzoite yield and ... | 2009 | 19027235 |
| factors affecting plasma prolactin concentrations throughout gestation in high producing dairy cows. | the aim of the present study was to investigate possible relationships between plasma concentrations of prolactin and the following factors throughout gestation in lactating dairy cows: photoperiod, season, milk production, neospora caninum-seropositivity, twin pregnancy, and plasma concentrations of pregnancy associated glycoproteins-1 (pag-1), progesterone and cortisol. pregnancy was diagnosed by trans-rectal ultrasonography on day 40 post-insemination and by palpation per rectum on days 90, 1 ... | 2009 | 19036554 |
| seroprevalence and spatial distribution of neospora caninum in a population of beef cattle. | a cross-sectional serological survey for neospora caninum was carried out in swedish beef cattle in order to estimate the seroprevalence and investigate any geographical patterns of the infection. blood samples were collected from 2754 animals in 2130 herds and analysed for presence of antibodies to n. caninum. the study included 18.5% of all swedish beef herds. spatial autocorrelation was assessed by calculating moran's i and cluster detection was performed using lisa- and spatial scan statisti ... | 2009 | 19709771 |
| seroprevalence of neospora caninum antibodies in beef cattle in three southern states of mexico. | the aims of the present study were to examine the seroprevalence of neosporosis in beef herds from three southern states of mexico and determine the association with several risk factors. a cross-sectional serological survey for neospora caninum was carried out by sampling a total of 596 animals from 31 herds in chiapas, veracruz, and yucatan states and tested using an elisa assay (idexx) to detect anti-n. caninum antibodies. the overall prevalence was 11.6%, (95% ci: 0.93-0.14), however, the pr ... | 2009 | 19016341 |
| frequency of horizontal and vertical transmission for sarcocystis cruzi and neospora caninum in dairy cattle. | sarcocystis cruzi and neospora caninum infections in cattle are common throughout the world, and cause important economical losses. n. caninum can be transmitted horizontally by ingestion of oocysts or vertically from the infected dam to the fetus via the placenta. vertical transmission for s. cruzi is infrequent and horizontal transmission is considered the most important route of infection. the objectives of this study were to evaluate the frequency of horizontal and vertical transmission for ... | 2009 | 19070964 |
| evaluation of a srs2 sandwich commercial enzyme-linked immunosorbent assay for the detection of anti-neospora caninum antibodies in bovine and canine sera. | neospora caninum is a parasite responsible for abortion in cows and neuromuscular disease in dogs. serology is the most widely used technique to evaluate the prevalence of n. caninum in different host populations. a sandwich enzyme-linked immunosorbent assay (elisa) based on the use of an anti-srs2 monoclonal antibody was evaluated against the indirect fluorescent antibody test for 100 canine sera and against a well-characterized elisa for 102 bovine sera. in cattle sera, the relative sensitivit ... | 2009 | 19139509 |
| investigation of neospora caninum, hammondia sp., and toxoplasma gondii in tissues from slaughtered beef cattle in bahia, brazil. | neospora caninum, hammondia sp., and toxoplasma gondii are parasites with morphological and genetic similarities. n. caninum and t. gondii are important abortive agents of cattle and sheep, respectively, and may infect numerous animal species. hammondia sp. is not known to induce disease in animals, but may cause confusion in the identification of closely related coccidia. the aim of this study was to investigate infection rates caused by n. caninum, hammondia sp., and t. gondii in beef cattle u ... | 2010 | 19943064 |
| seroprevalence of neospora caninum antibodies and its consequences for reproductive parameters in dairy cows from dakar-senegal, west africa. | the aim of this study was to determine the seroprevalence of neospora caninum antibodies and its effects on reproductive parameters in cows in intensive dairy herds in dakar. randomised blood samples were taken for serology from 196 cows in four herds with a history of sporadic abortion. all of the sera were assayed for antibodies against n. caninum, candida guillermondii, coxiella burnetii, and chlamydophila sp. the associations between serostatus and reproductive parameters (abortion, number o ... | 2010 | 19997972 |
| seroprevalence of antibodies to neospora caninum in bos javanicus ('bali cattle') from indonesia. | a cross-sectional survey was performed to obtain first information on the presence of neospora caninum infection in bos javanicus ('bali cattle'), the predominant beef cattle in the eastern islands of indonesia. serum samples were collected from 438 bali cattle of two age classes (<2 years, >2 years) and both genders at three slaughterhouses in the bali island, and examined for n. caninum-specific antibodies using native ncsrs2 (p38 antigen) as an elisa antigen. the estimated overall seroprevale ... | 2010 | 19533406 |
| the prevalence and spatial clustering of neospora caninum in dairy herds in norway. | the aims of this study were to estimate the national herd prevalence and the general spatial distribution of neospora caninum infection in norwegian dairy cattle. bulk milk samples from 1657 randomly selected dairy herds were analysed by iscom elisa for the presence of n. caninum specific antibodies. the national herd prevalence was estimated to be 0.7% (95% ci: 0.3-1.2%), which is the lowest national herd prevalence reported in europe. in addition, we included a subpopulation study in which all ... | 2010 | 20227188 |
| neospora caninum: early immune response of rat mixed glial cultures after tachyzoites infection. | neospora caninum causes neurologic disease in dogs and abortion in cattle. little is known about the immune response of the cns against this protozoan. the aim of this study was to evaluate production of il-6, il-10, tnf-alpha, ifn-gamma, and no in rat mixed glial cell cultures infected by n. caninum. ifn-gamma was not observed. the mean cytokine released after 24 and 72 h of infection were 3.8+/-0.6 and 3.7+/-0.6 pg tnf-alpha/mg protein and 2.7+/-0.69 and 4.1+/-0.64 pg il-10/mg protein, respect ... | 2010 | 20045699 |
| immune response to neospora caninum native antigens formulated with immune stimulating complexes in calves. | the aim of this study was to compare the immune responses to live neospora caninum tachyzoites and n. caninum native antigens formulated with immune stimulating complexes matrix (iscom-matrix) in calves. fifteen calves were used in this study: 3 were intravenously inoculated with 1 × 10(8) live tachyzoites (group a), 3 were inoculated twice with n. caninum native antigens formulated with iscoms (group b); 3 with n. caninum native antigens in phosphate-buffered saline (pbs) (group c); 3 received ... | 2010 | 21074325 |
| seroprevalence of neospora caninum in free living and farmed red deer (cervus elaphus) in poland. | serum samples from 47 free living and 106 farmed red deer (cervus elaphus) from the mazurian lake district in north-east poland were investigated for the presence of antibodies to neospora caninum. a modified neospora iscom-elisa was used for initial screening. all sera with optical density (od) values exceeding 0.400 absorbance units were further investigated by western blot analysis. eighteen sera were positive in both tests. six of these were from free living and 12 from farmed animals giving ... | 2010 | 21077439 |
| prevalence of antibodies against neospora caninum in dogs from urban areas in central poland. | neospora caninum is a protozoan parasite which causes abortion in cattle as well as reproduction problems and neurological disorders in dogs. to assess the prevalence of the parasite in urban dogs in the mazovian voivodeship, central poland, serum samples from 257 dogs were analyzed for the presence of specific igg antibodies. the examined dogs visited three private veterinary clinics located in warsaw due to control tests, vaccinations, or other reasons not directly connected with neosporosis. ... | 2010 | 21079995 |
| detection of specific antibodies anti-neospora caninum in the fallow deer (dama dama). | sera from 335 farmed fallow deer (dama dama) at the breeding station in kosewo górne in the mazurian lake district, north-east poland, were investigated for the presence of antibodies against neospora caninum. the distribution of age groups was as follow: >4years - 154 animals; 2years - 76 animals; 1year - 105 animals. ten sera with the optical density exceeding 0.159 absorbance units (i.e., cut-off value) in elisa test were also analyzed by western blot. western blot analysis revealed seroreact ... | 2010 | 21087781 |
| experimental infection of dogs (canis familiaris) with sporulated oocysts of neospora caninum. | neospora caninum is widely distributed in the world and this parasite is one of the major causes of abortion in cattle. dogs and coyotes are definitive hosts of n. caninum and several species of domestic and wild animals are intermediate hosts. dogs can become infected by the ingestion of tissues containing cysts and then excrete oocysts. it is not yet known whether sporulated oocysts are able to induce a patent infection in dogs, i.e. a shedding of n. caninum oocysts in feces. the objective of ... | 2010 | 21094584 |
| monensin use against neospora caninum challenge in dairy cattle. | using a randomized controlled trial design, a randomly allocated intervention group of 15 cows received a slow-release bolus that delivered 100 days of monensin. the negative control group of 15 cows received a placebo bolus that was identical to the monensin bolus, except without the monensin. two weeks after bolus administration, all cows were challenged with a 2 ml subcutaneous injection of a live tachyzoite suspension. whole blood and serum samples were collected from each cow every week for ... | 2010 | 21106293 |
| neospora caninum seroprevalence in dairy and beef cattle from the northwest region of spain, galicia. | herd and individual animal seroprevalence for neospora caninum (n. caninum) in dairy, beef and mixed cattle were obtained in all populations within the galician farmer sanitary defence associations (adsg) in 2004. all animals ≥1 year of age were examined serologically by indirect elisa. 1147 dairy herds (37,090 animals), 1464 beef herds (20,206 animals) and 141 mixed herds (2292 animals) were surveyed. true herd seroprevalence was estimated to be 80.6% (87.7% dairy, 76.7% beef and 78.4% mixed he ... | 2010 | 21145605 |
| development of an indirect elisa-ncsrs2 for detection of neospora caninum antibodies in cattle. | neosporosis is of alarming economic concern in the cattle industry. the effectiveness of diagnostic tests for detecting specific antibodies against neospora caninum is hampered by potential cross-reaction with other coccidia. use of a single specific antigen might improve test specificity. an indirect enzyme-linked immunosorbent assay (elisa) was developed using the truncated protein ncsrs2 expressed in escherichia coli. the elisa results were compared with those of the indirect fluorescence ant ... | 2010 | 21168278 |
| tunicamycins, a class of nucleoside antibiotics similar to corynetoxins of the rathayibacter toxicus, increase susceptibility of mice to neospora caninum. | neosporosis is the leading cause of abortion in cattle. neospora caninum-associated abortion may exhibit both endemic and epidemic patterns. it was reported that the epidemic outbreaks took place in the form of "abortion storms" and were not significantly correlated with seasonal changes or consumption of any particular feeds; and thus, the mechanisms by which the epidemic "abortion storms" are triggered remain unclear. annual ryegrass toxicity (argt) is a severe or fatal neurological disorder o ... | 2010 | 21168974 |
| influence of culture medium ph on internalization, growth and phenotypic plasticity of neospora caninum. | neospora caninum, a strictly intracellular protozoan, is a major leading cause of parasite-induced abortion in cattle. a widely held view of n. caninum infection is that both cellular proliferation and stage interconversion (tachyzoite-bradyzoite transformation) are triggered, perhaps even modulated by, changes in cultural conditions. this study tested the hypothesis that exposure of n. caninum tachyzoites to different ph culture media affects the parasite's entry, proliferation and cyst formati ... | 2010 | 21185654 |
| seroprevalence of neospora caninum infection in dairy cattle in northeastern china. | the seroprevalence of neospora caninum infection in china's northeastern heilongjiang province was surveyed between 2007 and 2008. in total, 540 serum samples of dairy cattle from 9 counties were examined for antibodies to n. caninum by enzyme-linked immunosorbent assay (elisa). the overall seroprevalence of n. caninum in dairy cattle was 13.3% (72/540). the seroprevalence of n. caninum in aborting cows (14.9%) was slightly higher than that in nonaborting cows (10.3%). dairy cattle with 5 pregna ... | 2010 | 19895158 |
| first identification of neospora caninum by pcr in aborted bovine foetuses in romania. | the first identification of neospora caninum infection in aborted bovine foetuses in romania is reported. nine aborted foetuses were collected from a dairy farm. the foetal age of foetuses was between 3 and 7 months. a 7-month-old foetus was mummified. n. caninum dna nc-5 region was amplified from samples extracted from brain tissues of three (33.3%) aborted foetuses. the foetal ages of pcr positive foetuses were 3, 4 and 7 months (mummified). no specific lesions or tissue cysts were found to th ... | 2010 | 19953273 |
| risk factors associated with neospora caninum seropositivity in randomly sampled canadian dairy cows and herds. | our objective was to determine cow- and herd-level risk factors associated with seropositivity for neospora caninum in a large number of randomly selected canadian dairy herds, controlling for important confounding variables and co-infections with bovine leukemia virus (blv), bovine viral diarrhea virus (bvdv) and mycobacterium avium subspecies paratuberculosis (map). serum samples were obtained from 30 randomly selected cows, where available, in 240 herds using monthly milk testing, within 6 of ... | 2010 | 20018395 |
| prevalence of neospora caninum antibodies in sheep flocks of uberlândia county, mg. | neosporosis is a parasitic disease that occurs in sheep and is associated with reproductive failure. the aim of this study was to verify seroprevalence of neospora caninum antibodies in 12 sheep flocks in uberlândia county, minas gerais, brazil, using indirect immunofluorescence assay (ifa). a total of 334 sheep blood samples were analyzed and an epidemiologic questionnaire was applied for each farm in order to correlate with risk factors of neosporosis: gender, age, breed, abortion problems, co ... | 2010 | 20943017 |
| neospora caninum excreted/secreted antigens trigger cc-chemokine receptor 5-dependent cell migration. | neospora caninum, the causative agent of neosporosis, is an obligate intracellular parasite considered to be a major cause of abortion in cattle throughout the world. most studies concerning n. caninum have focused on life cycle, seroepidemiology, pathology and vaccination, while data on host-parasite interaction, such as host cell migration, mechanisms of evasion and dissemination of this parasite during the early phase of infection are still poorly understood. here we show the ability of excre ... | 2010 | 20060395 |
| prevalence of antibodies against toxoplasma gondii and neospora caninum in moose (alces alces) and roe deer (capreolus capreolus) in sweden. | toxoplasma gondii and neospora caninum are two coccidian parasites with a worldwide distribution. t. gondii is one of the more common parasitic zoonoses in the world and in young children and immunocompromised persons, infection can lead to severe disease and death. n. caninum is an important cause of abortions in cattle. wildlife have been identified as reservoirs and transmitters for both parasites. the purpose of this study was to investigate the seroprevalences of t. gondii, and n. caninum i ... | 2010 | 21232869 |
| seroprevalence of neospora caninum infection in dairy cattle of southern china. | a seroepidemiological survey of neospora caninum infection in dairy cattle was carried out in china's southern guangdong province between july 2009 and march 2010. a total of 370 serum samples of dairy cattle was collected from 5 farms and examined for antibodies to n. caninum by enzyme-linked immunosorbent assay. the overall seroprevalence of n. caninum in dairy cattle was 18.9% (70/370). the seroprevalence of n. caninum in aborting cows (22.7%) was higher than that in nonaborting cows (16.3%), ... | 2010 | 21348632 |
| seroprevalence of toxoplasma gondii and neospora caninum in wild and domestic ruminants sharing pastures in galicia (northwest spain). | the prevalence of antibodies to the protozoan parasites toxoplasma gondii and neospora caninum were investigated by the direct agglutination test (dat) and celisa, respectively, in 160 roe deer (capreolus capreolus), 177 sheep and 178 cattle sharing pastures in galicia (northwest spain). the seroprevalence for t. gondii was 13.7% in roe deer, 57% in sheep and 7.3% in cattle. the seroprevalence for n. canimum was 6.8%, 10.1% and 24.1% in roe deer, sheep and cattle, respectively. statistically sig ... | 2010 | 19482324 |
| some factors affecting the abortion rate in dairy herds with high incidence of neospora-associated abortions are different in cows and heifers. | the aim of this study was to determine if the factors affecting the abortion rate in dairy herds with high incidence of neospora-associated abortions are different in pregnancies of cows and heifers chronically infected with neospora caninum. in heifers (n = 229), an increase in the cumulative number of days with a mean relative humidity (rh) lower than 60% during the second trimester of gestation increases the risk of abortion. yet, the likelihood of abortion was 7.6 times lower for pregnant he ... | 2010 | 19210662 |
| different humoral mechanisms against neospora caninum infection in purebreed and crossbreed beef/dairy cattle pregnancies. | the antigen-specific igg subclass response may be a convenient indicator of the underlying nature of t helper cell regulation. the aim of the present study was to identify possible differences in neospora caninum-specific total plasma igg, igg1 and igg2 antibody levels in purebreed and crossbreed pregnancies throughout gestation in beef and dairy cattle chronically infected with n. caninum. comparisons were also made between aborting and non-aborting dams. the population examined comprised 96 pr ... | 2010 | 21216103 |
| microsatellite typing and avidity analysis suggest a common source of infection in herds with epidemic neospora caninum-associated bovine abortion. | neosporosis is an important cause of reproductive failure in cattle worldwide. two different abortion patterns associated with neospora caninum infection have been observed in cattle herds: endemic and epidemic abortion outbreaks. the endemic pattern is characterized by an abortion problem in a herd persisting for several months or years, and is assumed to be caused by reactivation of a chronic infection. in epidemic outbreaks, abortions concentrate within a short period of time, most likely due ... | 2010 | 20609521 |
| association between seropositivity for neospora caninum and reproductive performance of beef heifers in the pantanal of mato grosso do sul, brazil. | neospora caninum is an obligate intracellular parasite that can infect domestic and wild canids, ruminants, and horses. the purpose of this study was to evaluate the association between the presence of antibodies to n. caninum and reproductive loss in beef heifers in the south pantanal region of mato grosso, brazil. a total of 1098 heifers were evaluated from breeding to calving with regard to reproductive performance, and serology for neosporosis was assayed by indirect immunofluorescence react ... | 2010 | 20624350 |
| fetal death in cows experimentally infected with neospora caninum at 110 days of gestation. | neospora caninum is a major cause of abortion in cattle, but the reasons why some animals abort and not others remain unclear. most of the n. caninum experimental primary infections in cattle late in gestation, after 120 days of pregnancy, result in birth of full-term congenitally infected fetuses. in the present study, the distribution of parasites and pathogenesis of infection in both dams and fetuses after inoculation with 10(7) culture derived tachyzoites of n. caninum nc-illinois cattle str ... | 2010 | 20089361 |
| association between neospora caninum seropositivity and perinatal mortality in dairy heifers at first calving. | associations between neospora caninum infection and the reproductive performance of dairy heifers in their first and second pregnancy on 18 uk farms were examined. six-month-old heifer calves were tested for n caninum-specific antibodies with a commercial elisa, and were then monitored until their second calving. random-effects regression analyses were performed on the data, using serological status as the explanatory variable. of 460 heifers tested, 7.2 per cent were seropositive. seropositive ... | 2010 | 20643884 |
| serologic profiles for sarcocystis sp. and neospora caninum and productive performance in naturally infected beef calves. | sarcocystis sp. and neospora caninum infections affect cattle worldwide causing important economic losses. the objective of the present study was to trace serologic profiles for sarcocystis sp. and n. caninum in naturally infected beef calves and analyze their relationship with transmission routes and productive performance. samples were collected in two cow-calf operations located in buenos aires province, argentina. in farm 1, 43 calves were bled and weighed three times. in farm 2, 69 calves w ... | 2010 | 20101411 |
| pathogenic characterization in mice of neospora caninum isolates obtained from asymptomatic calves. | in this study, we characterized 8 new isolates obtained from healthy but congenitally infected calves using a balb/c mouse model. neospora caninum-infected mice survived without exhibiting any clinical signs of disease. nevertheless, differences among isolates in parasite organ distribution, parasite burden and the severity of histopathological lesions were determined. mice infected with the nc-spain 5h, nc-spain 7 and nc-spain 9 isolates showed higher parasite burdens and more severe brain lesi ... | 2010 | 20233488 |
| australian dingoes are definitive hosts of neospora caninum. | to provide objective data on the potential role of dingoes (canis lupus dingo) in the life cycle of neospora caninum in australia, the production of n. caninum oocysts by experimentally infected canids was investigated. three dingo pups raised in captivity and three domestic dogs were fed tissue from calves infected with an australian isolate of n. caninum, nc-nowra. oocysts of n. caninum, confirmed by species-specific pcr, were shed in low numbers by one dingo pup at 12-14 days p.i. the remaini ... | 2010 | 20149793 |
| a survey of neospora caninum-associated bovine abortion in large dairy farms of mashhad, iran. | neospora caninum is an apicomplexan protozoon causing abortion in cattle worldwide. the present study was designed to assess the importance of this parasite for causing abortion in dairy farms in the mashhad area of iran. of the aborted bovine fetuses, 151 were collected from dairy farms between 2006 and 2008. first, brain samples were examined by polymerase chain reaction (pcr) for the presence of n. caninum dna, diagnosis was complemented with immunohistochemistry (ihc) and fetal serology (eli ... | 2010 | 20352453 |
| comparative evaluation of immunofluorescent antibody and new immunoblot tests for the specific detection of antibodies against besnoitia besnoiti tachyzoites and bradyzoites in bovine sera. | besnoitia besnoiti, an apicomplexan parasite causes economically important disease in cattle in many countries of africa and asia is re-emerging in europe. serological identification of infected cattle is important because introduction of these animals into naive herds seems to play a major role in the transmission of the parasite. we report new, simplified immunoblot-based serological tests for the detection of b. besnoiti-specific antibodies. antigens were used under non-reducing conditions in ... | 2010 | 20378250 |
| abortions in bovines and neospora caninum transmission in an embryo transfer center. | neosporosis is considered to be one of the main causes of abortions in bovines. in this study we evaluated the congenital transmission and occurrence of abortions by neospora caninum in an embryo transfer center in neropolis, goiás. serological samples from 101 recipients, 61 donors, and 90 calves were analyzed. among these animals, 32.67% of the recipients, 22.22% of the donors, and 6.66% of the calves were positive for n. caninum. the rate of vertical transmission was 24%. there was a statisti ... | 2010 | 20655663 |
| identification of neospora caninum proteins regulated during the differentiation process from tachyzoite to bradyzoite stage by dige. | identification of differentially expressed proteins during neospora caninum tachyzoite-bradyzoite conversion processes may lead to a better knowledge of the pathogenic mechanisms developed by this important parasite of cattle. in the present work, a differential expression proteomic study of tachyzoite and bradyzoite stages was accomplished for the first time by applying dige technology coupled with ms analysis. up to 72 differentially expressed spots were visualized (1.5-fold in relative abunda ... | 2010 | 20162558 |
| kennel dogs as sentinels of leishmania infantum, toxoplasma gondii, and neospora caninum in majorca island, spain. | kennel dogs can serve as sentinels and/or reservoirs of diseases of veterinary and zoonotic interest because they have often roamed free and lived outdoors, thus being exposed to pathogens. we tested dogs from the kennel of inca (majorca/mallorca, balearic islands, spain) for evidence of infection with three protozoan parasites: leishmania infantum, toxoplasma gondii, and neospora caninum. exposure to l. infantum was found in 56.3% of 48 dogs (37.5% by western blot, 43.8% by pcr). only 30% of in ... | 2010 | 20689966 |
| recognition by toll-like receptor 2 induces antigen-presenting cell activation and th1 programming during infection by neospora caninum. | neospora caninum is an apicomplexan parasite responsible for major economic losses due to abortions in cattle. toll-like receptors (tlrs) sense specific microbial products and direct downstream signaling pathways in immune cells, linking innate, and adaptive immunity. here, we analyze the role of tlr2 on innate and adaptive immune responses during n. caninum infection. inflammatory peritoneal macrophages and bone marrow-derived dendritic cells exposed to n. caninum-soluble antigens presented an ... | 2010 | 20404835 |
| differential susceptibility of human trophoblastic (bewo) and uterine cervical (hela) cells to neospora caninum infection. | neospora caninum is an apicomplexan parasite, closely related to toxoplasma gondii, and causes abortion and congenital neosporosis in cattle worldwide. trophoblast cells act in mechanisms of innate immune defense at the fetal-maternal interface and no data are available about the interaction of neospora with human trophoblasts. thus, this study aimed to verify the susceptibility of human trophoblastic (bewo) compared with uterine cervical (hela) cell lines to n. caninum. bewo and hela cells were ... | 2010 | 20708622 |
| neospora caninum and coxiella burnetii seropositivity are related to endocrine pattern changes during gestation in lactating dairy cows. | q fever is a zoonotic infection caused by coxiella burnetii that is endemic worldwide. domestic ruminants are a source of infection for humans. given the suggestion that the bacterium recrudesces during pregnancy in cattle, this study was designed to determine whether c. burnetii infection affects hormonal patterns, such as progesterone, cortisol, pregnancy associated glycoproteins (pag), and prolactin during gestation in lactating cows. possible interactions with neospora caninum were also expl ... | 2010 | 20416940 |
| neospora caninum tachyzoite- and antigen-stimulated cytokine production by bone marrow-derived dendritic cells and spleen cells of naive balb/c mice. | neospora caninum is an intracellular protozoan pathogen that causes abortion in cattle. this parasite elicits a typical type 1 immune response in host animals, and it is widely believed that the strong type 1 immune response during pregnancy may result in fetal death. pro-inflammatory and/or inflammatory cytokines produced during either primary or secondary pathogen exposure are supposed to be the mediators of abortion. the present study defined cytokine production by murine naïve dendritic cell ... | 2010 | 20738198 |
| associations between reproductive performance and seropositivity for bovine leukemia virus, bovine viral-diarrhea virus, mycobacterium avium subspecies paratuberculosis, and neospora caninum in canadian dairy cows. | our objective was to investigate effects of seropositivity for bovine leukemia virus (blv), type 1 bovine viral-diarrhea virus (bvdv), mycobacterium avium subspecies paratuberculosis (map), and neospora caninum (nc), and their possible interactions, on reproductive efficiency (specifically, first-service conception [fsc], and calving interval [ci]) in dairy cows. the sample population included up to 30 randomly selected animals from 179 randomly selected farms in five provinces in canada, from w ... | 2010 | 20015556 |
| stress-driven stage transformation of neospora caninum. | neosporiosis, a serious disease caused by the apicomplexan intracellular parasite neospora caninum, is considered to be one of the most economically important diseases in cattle. it is associated with potentially serious complications such as abortion, stillbirth and maternal infertility. to survive in fluctuating physiological and immunological environments, n. caninum has evolved a diverse set of regulatory mechanisms that govern various adaptive responses. the most intriguing paradigm in n. c ... | 2010 | 20165871 |
| microarray analyses of mouse responses to infection by neospora caninum identifies disease associated cellular pathways in the host response. | neospora caninum is a coccidian cyst-forming parasite found in a wide range of host species such as mice, dogs and cattle. the development of methods such as vaccines to prevent abortion and fetal loss due to neosporosis would be greatly assisted by further knowledge on immunity and host responses to infection. in this study we used microarray technology to investigate the protective host responses occurring at 6h post infection in the spleen of mice infected with a prototype live n. caninum vac ... | 2010 | 20817048 |
| neospora caninum: evaluation of vertical transmission in slaughtered beef cows (bos indicus). | neospora caninum is a protozoan parasite that causes the most important reproductive problems in cattle worldwide. the objective of this study was to evaluate the possibility of vertical transmission of n. caninum in zebus breed beef cows (bos indicus) submitted for slaughter at an abattoir in the northern region of the state of paraná, southern brazil. one hundred and fifty-nine cows were evaluated: 83 pregnant (in different stages of gestation) and 76 non-pregnant. serum determination of n. ca ... | 2010 | 21063729 |
| a new thrombospondin-related anonymous protein homologue in neospora caninum (ncmic2-like1). | neospora caninum is an apicomplexan protozoan that has the dog as a definitive host and cattle (among other animals) as intermediate hosts. it causes encephalopathy in dogs and abortion in cows, with significant loss in worldwide livestock. as any apicomplexan, the parasite invades the cells using proteins contained in the phylum-specific organelles, like the micronemes, rhoptries and dense granules. the aim of this study was the characterization of a homologue (denominated ncmic2-like1) of n. c ... | 2010 | 20880420 |
| evaluation of a commercial elisa for the specific detection of antibodies against besnoitia besnoiti. | bovine besnoitiosis is an economically important disease in cattle caused by the protozoan parasite besnoitia besnoiti, which occurs endemically in many countries of africa and asia and is spreading in europe. serological identification of subclinically infected cattle is important to avoid the introduction of infected animals into naive herds. here we determine the sensitivity and specificity of the priocheck(®) besnoitia ab, a serological test recently introduced into the european market. anal ... | 2010 | 21035269 |
| neosporosis in animals--the last five years. | neospora caninum is a protozoan parasite of animals. until 1988, it was misdiagnosed as toxoplasma gondii. since its first recognition in 1984 in dogs and the description of a new genus and species neospora caninum in 1988, neosporosis has emerged as a serious disease of cattle and dogs worldwide. abortions and neonatal mortality are a major problem in livestock operations and neosporosis is a major cause of abortion in cattle. this review is focused on current status of neosporosis in animals b ... | 2011 | 21704458 |
| epidemiology and impact of neospora caninum infection in three queensland tropical dairy herds. | this study investigated the epidemiology of neospora caninum in three tropical dairy herds in north queensland, australia. all animals in the herds were bled, and the sera were tested by elisa for n. caninum antibodies. herd records were examined, and the number of calves carried to term and the number of abortions which occurred over the lifetime of each animal were recorded to determine the abortion rate for each animal. pedigrees were constructed for two of the herds to investigate whether ve ... | 2011 | 21736635 |
| transgenic neospora caninum strains constitutively expressing the bradyzoite ncsag4 protein proved to be safe and conferred significant levels of protection against vertical transmission when used as live vaccines in mice. | at present, there is no effective treatment or vaccine to prevent vertical transmission or abortion associated with neospora caninum infection in cattle. different vaccine formulations have been assayed, and live vaccines have shown the most promising results in terms of protection. previously, transgenic n. caninum tachyzoites expressing the bradyzoite stage-specific ncsag4 antigen in a constitutive manner (nc-1 sag4(c)) were obtained and showed a reduced persistence of parasite in inoculated m ... | 2011 | 21816191 |
| survey for natural neospora caninum infection in wild and captive birds. | neospora caninum is a protozoan parasite that presents worldwide distribution and is mainly implicated as responsible for bovine abortion. although the presence of birds in cattle-raising properties is positively correlated to higher infection rates, very little has been described about the role of these animals in the parasite's life cycle. in that sense, this work aimed to investigate the serological and histological positivity of different avian species sampled in its natural habitat or in ca ... | 2011 | 21680099 |
| neospora caninum is associated with abortion in algerian cattle. | abstract neospora caninum is a major cause of abortion in cattle worldwide. however, little information is available for algeria. accordingly, 799 cattle from 87 farms in the north and northeast of algeria were enrolled in a seroepidemiological survey. an indirect fluorescence antibody test (ifat) revealed a seroprevalence of 19.6%. the animals were divided into 3 groups according to their breed: imported european cattle, local breeds, and crossed animals (european x local). seroprevalences were ... | 2011 | 21728878 |
| first report of neospora caninum infection in cattle in sudan. | a cross-sectional survey was conducted in sudan to determine sero-prevalence and risk factors associated with neospora caninum infection in non-vaccinated dairy herds and to assess importance of the disease. blood samples were collected from a total of 262 animals from 25 herds. sera were tested for antibodies against n. caninum using elisa test. the prevalence rates of n. caninum antibodies in cattle were high both at herd level (44%) and at individual animal level (10.7%). herd level infection ... | 2011 | 21898183 |
| seroprevalence of neospora caninum in dairy cattle from north-west and centre of romania. | neosporosis is a disease that mainly affects cattle in both dairy and beef herds. the main definitive host of this parasite is the dog. since 1984 and its first description a large number of data were published worldwide on this parasite. in romania, the research regarding this parasite is limited. the purpose of this study was to determine the seroprevalence of neospora caninum infection in dairy cattle from six regions in north-western romania and to evaluate the intensity of infection in diff ... | 2011 | 22091468 |
| an applied printing immunoassay with recombinant nc-sag1 for detection of antibodies to neospora caninum in cattle. | neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. neospora caninum surface antigen 1 (nc-sag1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. the current study describes the development and evaluation of an antigen print immunoassay (apia) with recombinant nc-sag1 for the detection of specific antibodies to n. caninum in cattle. the concordance between apia and a commercial enzyme-linked immunosorbent as ... | 2011 | 21908357 |
| Parasite limiting macrophages promote IL-17 secretion in naive bovine CD4? T-cells during Neospora caninum infection. | Neospora caninum infects bovine hosts giving rise to pro-inflammatory immune responses that can result in foetal death or spontaneous abortion, this appears to be mediated by the actions of IFN-? on cell activation and migration/trafficking. Yet successful vaccination or natural immunity is also strongly correlated with IFN-? production. We utilised in vitro infection of bovine macrophages to prime naive T-cell responses. Naive T-cells in contact with infected macrophages produce both IFN-? and ... | 2011 | 22005586 |
| Development of a set of multiplex standard polymerase chain reaction assays for the identification of infectious agents from aborted bovine clinical samples. | The current study describes the development of a set of 5 multiplex polymerase chain reaction (mPCR) assays for the simultaneous detection of abortive infection agents in bovine fetal tissues, including Brucella spp., Leptospira spp., and Campylobacter fetus (mPCR1); Hammondia heydorni, Neospora caninum, and Toxoplasma gondii (mPCR2); Coxiella burnetii and Chlamydophila psittaci (mPCR3); Mycoplasma bovis, Mycoplasma bovigenitalium, and Ureaplasma diversum (mPCR4); and Bovine viral diarrhea virus ... | 2011 | 21908306 |
| serological survey of neospora caninum in small ruminants from pernambuco state, brazil. | neospora caninum infection is an important cause of bovine abortion as well as neonatal mortality in goats and sheep. a serological survey for antibodies against n. caninum in goats and sheep was carried out in the municipality of ibimirim, pe, northeastern brazil. the imunnofluoresce antibody test showed that 26.6% (85/319) of the goats and 64.2% (52/81) of the sheep were positive. serologic reactivity was associated with age in goats (p < 0.01) and sheep (p > 0.05), with increasing rates in ol ... | 2011 | 21961757 |
| artinm, a d-mannose-binding lectin from artocarpus integrifolia, plays a potent adjuvant and immunostimulatory role in immunization against neospora caninum. | artinm and jacalin (jac) are lectins from the jackfruit (artocarpus integrifolia) that have important role in modulation of immune responses to pathogens. neospora caninum is an apicomplexa parasite that causes neuromuscular disease in dogs and reproductive disorders in cattle, with economic impact on the livestock industry. hence, we evaluated the adjuvant effect of artinm and jac in immunization of mice against neosporosis. six c57bl/6 mouse groups were subcutaneously immunized three times at ... | 2011 | 22001880 |
| serological survey of neospora caninum infection in cattle herds from western romania. | abstract serum samples from 376 randomly selected adult cattle, from 25 farms located in 3 counties (arad, bihor, and timiş) from western romania, were sampled for neospora caninum antibodies using a commercial elisa-kit (bio k 218, bio-x diagnostics, jemelle, belgium). seroprevalence values and risk factors for neosporosis (cow age, breed, herd size, farming system, previous abortion, and number of farm dogs) were examined using a generalized linear mixed model with a binomial distribution. o ... | 2011 | 22150344 |
| peripheral white blood cell counts throughout pregnancy in non-aborting neospora caninum-seronegative and seropositive high-producing dairy cows in a holstein friesian herd. | pregnancy is characterized by transient changes in the maternal immune system, also evident at peripheral level. the present study analyzes the kinetics and possible factors affecting peripheral white blood cell populations throughout pregnancy in a herd of high-producing dairy cows chronically infected or not with neospora caninum. we examined 54 pregnant parous cows: 29 neospora-seronegative and 25 neospora-seropositive cows. blood samples were collected on days 90, 120, 150, 180 and 210 of ge ... | 2011 | 20801467 |
| implications of wild dog ecology on the sylvatic and domestic life cycle of neospora caninum in australia. | neospora caninum is transmitted either transplacentally or horizontally by ingestion of tissue cysts present in tissues or oocysts shed by dogs. neosporosis is a significant disease, causing cattle abortion at 5-7 months of pregnancy. infected cows may remain infective for life transmitting the infection in several consecutive or non-consecutive pregnancies. a great deal is known about the epidemiology of neosporosis, although only limited information is available on the main routes of horizonta ... | 2011 | 20400345 |
| genetic manipulation of neospora caninum to express the bradyzoite-specific protein ncsag4 in tachyzoites. | neospora caninum is an apicomplexan parasite and the aetiological agent of bovine neosporosis, one of the main causes of reproductive failure worldwide. we have generated 2 independent transgenic knock-in clones, nc-1sag4c1.1 and nc-1sag4c2.1, that express the bradyzoite stage-specific protein ncsag4 in the tachyzoite stage. these clones have similar growth rates in vitro as the wild-type (wt) strain nc-1. studies in a cerebral mouse model of infection revealed a slightly lower rate of detection ... | 2011 | 21232176 |
| neospora caninum: comparative gene expression profiling of neospora caninum wild type and a temperature sensitive clone. | to understand the genetic basis of virulence, gene expression profiles of a temperature-sensitive clone (ncts-8, relatively avirulent) and its wild type (nc-1) of neospora caninum were characterized and compared using a high-density microarray with approximately 63,000 distinct oligonucleotides. this microarray consists of 5692 unique n. caninum sequences, including 1980 tentative consensus sequences and 3712 singleton ests from the tigr n. caninum gene index (ncgi, release 5.0). each sequence w ... | 2011 | 21963790 |
| Molecular pathology, taxonomy and epidemiology of Besnoitia species (Protozoa: Sarcocystidae). | Until recently, besnoitiosis has been a neglected disease of domestic animals. Now, a geographic expansion of the causing protozoan parasite Besnoitia besnoiti in livestock has been recognized and the disease in cattle is considered emerging in Europe. Bovine besnoitiosis leads to significant economic losses by a decline in milk production, sterility, transient or permanent infertility of bulls, skin lesions and increase of mortality in affected cattle population. Phylogenetically, the Besnoitia ... | 2011 | 21906696 |
| sero-prevalence of neospora caninum and besnoitia besnoiti in south australian beef and dairy cattle. | a serological survey for neospora caninum and besnoitia besnoiti was carried out in beef and dairy cattle in south australia. serum samples of dairy cattle (n=133) from 9 properties and tank milk samples from a further 122 dairy herds were tested. an additional 810 sera from beef cattle from 51 properties were also tested. testing at the individual animal level by idexx neospora x2 ab test elisa revealed a low prevalence of n. caninum antibodies of only 2.7% (95% ci; 1.6-3.7%) sera positive, as ... | 2011 | 22138306 |
| Potential involvement of Neospora caninum in naturally occurring ovine abortions in New Zealand. | Neospora caninum is an obligate intracellular parasite and is recognised as the leading cause of bovine abortion worldwide. Natural infection with N. caninum has been described in sheep but it has generally not been regarded as a significant cause of abortion. Recently, there have been several New Zealand cases of foetal abortions where N. caninum was detected which strongly suggested the involvement of Neospora in these abortions. However, there is minimal information about the prevalence of N. ... | 2011 | 22112976 |
| seroprevalence of neospora caninum infection on dairy cattle in farms from southern romania. | abstract neospora caninum, a coccidian parasite closely related to toxoplasma gondii, is one of the major causes of abortion in cattle worldwide. conventional serological techniques, such as indirect fluorescent antibody test and enzyme-linked immunosorbent assay (elisa), are routinely used in adult animals and aborted fetuses for the detection of anti-n. caninum antibodies. in romania, infection with n. caninum in cattle has been reported recently, but only in limited areas from the north and ... | 2011 | 21955278 |
| neospora caninum in bovine fetuses of minas gerais, brazil: genetic characteristics of rdna. | neosporosis is an important cause of abortion in cattle and information on their genetics and host parasite relationships are desirable. neospora caninum samples obtained from 24 bovine fetuses from minas gerais, were genetically analyzed in part of the rdna region, coding for rrnas. previously, brain, heart, liver, skeletal muscle, lung, kidney, spleen, thymus, lymph nodes, ovary or testis, uterus and skin of the ear were analyzed by conventional histopathology and immunohistochemistry. of thes ... | 2011 | 22166381 |
| neospora caninum: in vivo and in vitro treatment with artemisone. | neosporosis caused by neospora caninum has global economic, clinical, and epidemiological impacts, mainly in the cattle industry. currently, there is no useful drug for treatment of neosporosis. this publication is the first to describe the significant benefits that artemisone has on neospora infections both in vitro and in vivo. artemisone is a new semi-synthetic 10-alkylamino artemisinin that is superior to other artemisinin derivatives in terms of its significantly higher antimalarial activit ... | 2011 | 22260899 |
| experimental inoculation of neospora caninum in pregnant water buffalo. | the aim of this study was to characterize the pathogenesis of neospora caninum in experimentally inoculated pregnant water buffalo (bubalus bubalis). twelve mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to n. caninum by indirect fluorescent antibody test (ifat) were involved. ten females were intravenously inoculated with 10(8) tachyzoites of nc-1 strain at 70 (n=3) or 90 (n=7) days of pregnancy (dp). two control animals were inoculated with placebo ... | 2011 | 22244534 |